Search

Additional file 2: Supplementary Table 1. Compared with PL, High and low level expression for differentially abundant proteins in PA

Additional file 8: Supplementary Table 14. Primer of qPCR

Additional file 7: Supplementary Table 6. The differentially expressed proteins with the same name were merged among six groups. Supplementary Table 7. Extremely significant enrichment GO term both in PL-PA and PA-MET groups. Supplementary Table 8. Extremely significant enrichment GO term in PL-eSEN -einSEN groups. Supplementary Table 9. Extremely significant enrichment GO term in PL-MET -eMET groups. Supplementary Table 10. Extremely significant enrichment KEGG pathway of PL-MET group. Supplementary Table 11. Extremely significant enrichment pathway only in eSEN-eMET group against PL-eSEN group. Supplementary Table 12. Extremely significant enrichment pathway only in PL-einSEN group against PL-eSEN group. Supplementary Table 13. Protein abbreviations and corresponding full name

Additional file 6: Supplementary Table 5. The differentially expressed proteins among six groups with protein volumes calculated

Additional file 5: Supplementary Table 4. Compared with MET, High and low level expression for differentially abundant proteins in eMET

Additional file 4: Supplementary Table 3. Compared with PA, High and low level expression for differentially abundant proteins in e-SEN

Additional file 1: Supplementary Figure 1. The basic information of proteome identification. Supplementary Figure 2. The overall distribution of quantitative proteomics. A. Protein mass distribution; B. Peptide length distribution; C. Distribution of peptides’s sequence coverage; D. Distribution of unique peptide. Supplementary Figure 3. Gene ontology classification of oyster proteome. Supplementary Figure 4. KEGG function classification of oyster proteome. A-cellular processes, B-environmental information processing, C-genetic information processing, D-metabolism, E-organismal system. Supplementary Figure 5. Embryonic development of Fujian oyster Crassostrea angulate (A) Post eyespot larvae; (B) the collection box in Fujian oyster C. angulate; (C) the larvae after metamorphosis; (D) the larvae after metamorphosis induced by epinephrine.

(2R3S4S)-5-Fluoro-2,3,4-trihydroxypentanoic acid (5-FHPA) has been discovered as a new fluorometabolite in the soil bacterium Streptomyces sp. MA37., (2R3S4S)-5-Fluoro-2,3,4-trihydroxypentanoic acid (5-FHPA) has been discovered as a new fluorometabolite in the soil bacterium Streptomyces sp. MA37. Exogenous addition of 5-fluoro-5-deoxy-d-ribose (5-FDR) into the cell free extract of MA37 demonstrated that 5-FDR was an intermediate to a range of unidentified fluorometabolites, distinct from fluoroacetate (FAc) and 4-fluorothreonine (4-FT). Bioinformatics analysis allowed identification of a gene cluster (fdr), encoding a pathway to the biosynthesis of 5-FHPA. Over-expression and in vitro assay of FdrC indicated that FdrC is a NAD+ dependent dehydrogenase responsible for oxidation of 5-FDR into 5-fluoro-5-deoxy-lactone, followed by hydrolysis to 5-FHPA. The identity of 5-FHPA in the fermentation broth was confirmed by synthesis of a reference compound and then co-correlation by 19F-NMR and GC-MS analysis. The occurrence of 5-FHPA proves the existence of a new fluorometabolite pathway.