Search

Ionospheric oblique backscatter sounding is an effective means of monitoring the ionosphere which can be used as a frequency selection system to serve HF communication and ensure its quality and stability. But how to obtain effective information from the oblique backscatter ionogram is still a hot issue. Due to this situation, a frequency selecting method for HF communication based on ionospheric oblique backscatter sounding is proposed in this study. After obtaining the ionograms, pattern recognition is used to separate the vertical echoes and the oblique backscatter echoes. Next, the leading edge of the oblique backscatter echoes are extracted, and then a two-dimensional electron density profile can be reconstructed. Then, with the help of ray tracing, the usable frequency range can be estimated. Finally, according to the signal-to-noise ratio reflected by the ionograms, several optimal communication frequencies can be selected. In order to verify this method, oblique ionograms are obtained through oblique sounding experiments to evaluate its accuracy. The result indicates that the usable frequency range and the selected frequencies are in accordance with the echo of the oblique ionogram, so the practicability and accuracy of the method are validated. Eventually, the maximum usable frequencies (MUFs) obtained from oblique backscatter sounding are compared with the MUFs from the oblique sounding ionogram; its Mean Absolute Percentage Error (MAPE) is 7.8% and its root mean squared error (RMSE) is 1.34 MHz.

To investigate the effects of Pseudomonas monteilii SX001 on various parameters of cucumber plants under salt stress, the salt-sensitive cucumber variety “Jinyou No. 4” was used as the test material, and coconut bran was used to simulate salt stress by applying NaCl solution. The results indicated that salt stress significantly reduced the morphological structure, relative growth rate, root morphology, and photosynthetic parameters of the cucumber plants. Leaf starch, soluble sugar, and sucrose contents significantly increased, whereas their levels in roots decreased. Cell membrane damage leads to the accumulation of reactive oxygen species and malondialdehyde, with notable increases in the activities of major antioxidant enzymes such as SOD, CAT, and POD. Nitrogen metabolism was disrupted, as evidenced by a significant decrease in nitrate nitrogen content and an increase in ammonium nitrogen content, as well as a significant reduction in the activity of NR enzymes involved in nitrogen metabolism. The enzyme activity in the cucumber rhizosphere soil decreased. However, Pseudomonas monteilii SX001 significantly enhanced the growth of cucumber seedlings under salt stress, improved photosynthetic efficiency, and facilitated sugar transformation and transport via glucose metabolism. Additionally, Pseudomonas monteilii SX001 reduced the reactive oxygen content and increased antioxidant enzyme activity. It also increased the activity of substrate enzymes and decreased the diversity of rhizosphere soil microorganisms but also increased the abundance of Asticcacaulis, Acinetobacter, Brevundimonas, Pseudomonas, and Enterobacter. These findings demonstrate that Pseudomonas monteilii SX001 is a promising bioinoculant for alleviating salt stress in cucumber production and improving soil health.

Agricultural machinery is effective in improving food production and labor productivity, but it also raises environmental concerns. The impacts of agricultural machinery on the green total factor productivity (GTFP) of grain in China are still under debate in the scientific literature. This study proposed an integrated framework for confronting this issue. The findings suggest that both agricultural mechanization and the GTFP of grain demonstrate a consistent upward trend with moderate fluctuations between 2001 and 2019. By expanding the spatial pattern, there is a positive spatial correlation between them. In addition, we compared the results in three grain functional areas by using the spatial Durbin model (SDM). There were significantly positive spatial spillover effects in major grain-producing areas, which were attributed to the trans-regional operation of agricultural machinery and its carbon reduction effects on neighboring provinces. Notably, the direct effects in major grain-marketing areas and producing-marketing balance areas were significantly positive because agricultural machinery has played a critical role in filling the gap in local labor shortages in grain production. Accordingly, adaptive strategies including building the “Internet + agricultural machinery operation” platform, implementing the land consolidation suitable for machinery, and developing low-carbon agricultural machinery should be fully considered by Chinese policy-makers to promote mechanized agriculture and a low-carbon economy. The findings of this study can help us better understand the role of agricultural machinery in improving green grain productivity in China and thus have significance for the modern and green transformation of agricultural production systems.

Spread F (SF) in the ionosphere can be observed frequently in mid-latitude regions. It is suggested that atmospheric gravity waves play a significant role for the seeding of mid-latitude SF. Previous research suggested that the source of travelling ionospheric disturbances (TIDs) over China is in the southeastern and northeastern edge of the Qinghai-Tibet Plateau, however, until now there have been no ground-based observations of the ionosphere in this region. Recently, an advanced digital ionosonde was installed at Zhangye station (39.2°N, 100.54°E, Dip Lat 29.6°N) in the northeastern edge of the Qinghai-Tibet Plateau. It is an opportunity to verify the effect of gravity waves on the formation of mid-latitude SF by comparing it with observations in other regions of the Chinese sector. In this study, statistical analysis of SF recorded at Zhangye station during 2017–2022 was carried out. Results show that diurnal, seasonal and solar cycle characteristics of the occurrence rate of SF are similar with previous studies. At Zhangye station, the maximum occurrence rate of SF is during the post-midnight period in summer and winter. The occurrence rate of SF events have a negative relationship with solar activity. There is no obvious relationship between the occurrence rate of SF and geomagnetic activity. Comparing observations of other stations in the mid-latitude region, we found that the occurrence rates of SF (the annual maximum rates are from 33.83% to 53.29%) are much higher at Zhangye station. Further studies show that ionospheric disturbances can be observed frequently at Zhangye station, especially in autumn and winter. Gravity waves/TIDs in the northeast of the Qinghai-Tibet Plateau are suggested to explain the abnormal higher occurrence rate of SF at Zhangye station.

Soybean production is severely hampered by saline-alkaline stress caused by saline-alkalization. Plants have aldehydrogenase (ALDH) family members that convert reactive aldehydes to carboxylic acids to remove active aldehyde molecules. However, little is known about the increased saline-alkali tolerance caused by the ALDH function in soybean. Here, we introduced a previously identified ALDH coding gene AhALDH3H1 from Arachis hypogaea into the soybean genome to investigate its critical role in response to saline-alkali stress. Transgenic soybean with increased aldehyde dehydrogenase activity showed significant tolerance to saline-alkali stress. It reduced malondialdehyde (MDA) content compared to its receptor, suggesting that over-expression of AhALDH3H1 accelerated soybean tolerance to saline-alkali stress by increasing aldehyde dehydrogenase activity, which is responsible for scavenging toxic MDA. To further analyze the inner mechanisms that allow transgenic plants to tolerate saline-alkali stress, we sequenced the transcriptome and metabolome of P3 (wild type, WT) and transgenic lines which were separately treated with water and a saline-alkali solution. When subjected to saline-alkali stress, the integrated analysis of the transcriptome and metabolome suggested that several genes related to cell wall structure crucial for preserving cell wall extensibility and plasticity were largely responsible for restoring homeostasis within the transgenic cells compared to WT. Metabolites, including both necessary ingredients for cell wall genesis and harmful production produced during the saline-alkali stress response, could be transported efficiently with the help of the ABC transporter, reducing the negative effects of saline-alkali stress. These findings suggest that introducing AhALDH3H1 increases transgenic soybean tolerance to saline-alkali stress may through cell wall structure maintenance and metabolites transport.

Identifying available farmland suitable for agricultural machinery is the most promising way of optimizing agricultural production and increasing agricultural mechanization. Farmland consolidation suitable for agricultural machinery (FCAM) is implemented as an effective tool for increasing sustainable production and mechanized agriculture. By using the machine learning approach, this study assesses the suitability of farmland for agricultural machinery in land consolidation schemes based on four parameters, i.e., natural resource endowment, accessibility of agricultural machinery, socioeconomic level, and ecological limitations. And based on “suitability” and “potential improvement in farmland productivity”, we classified land into four zones: the priority consolidation zone, the moderate consolidation zone, the comprehensive consolidation zone, and the reserve consolidation zone. The results showed that most of the farmland (76.41%) was either basically or moderately suitable for FCAM. Although slope was often an indicator that land was suitable for agricultural machinery, other factors, such as the inferior accessibility of tractor roads, continuous depopulation, and ecological fragility, contributed greatly to reducing the overall suitability of land for FCAM. Moreover, it was estimated that the potential productivity of farmland would be increased by 720.8 kg/ha if FCAM were implemented. Four zones constituted a useful basis for determining the implementation sequence and differentiating strategies for FCAM schemes. Consequently, this zoning has been an effective solution for implementing FCAM schemes. However, the successful implementation of FCAM schemes, and the achievement a modern and sustainable agriculture system, will require some additional strategies, such as strengthening farmland ecosystem protection and promoting R&D into agricultural machinery suitable for hilly terrain, as well as more financial support.

ObjectiveTo explore the feasibility of predicting distant metastasis (DM) of nasopharyngeal carcinoma (NPC) patients based on MRI radiomics model.MethodsA total of 146 patients with NPC pathologically confirmed, who did not exhibit DM before treatment, were retrospectively reviewed and followed up for at least one year to analyze the DM risk of the disease. The MRI images of these patients including T2WI and CE-T1WI sequences were extracted. The cases were randomly divided into training group (n=116) and validation group (n=30). The images were filtered before radiomics feature extraction. The least absolute shrinkage and selection operator (LASSO) regression was used to develop the dimension of texture parameters and the logistic regression was used to construct the prediction model. The ROC curve and calibration curve were used to evaluate the predictive performance of the model, and the area under curve (AUC), accuracy, sensitivity, and specificity were calculated.Results72 patients had DM and 74 patients had no DM. The AUC, accuracy, sensitivity and specificity of the model were 0. 80 (95% CI: 0.72~0. 88), 75.0%, 76.8%, 73.3%. and0.70 (95% CI: 0.51~0.90), 66.7%, 72.7%, 63.2% in training group and validation group, respectively.ConclusionThe radiomics model based on logistic regression algorithm has application potential for evaluating the DM risk of patients with NPC.

BackgroundNeoadjuvant chemoradiotherapy (neo-CRT) plus surgery has greatly improved the prognosis of locally advanced esophageal cancer (EC) patients. But which factors may influence the pathological tumor response and long-term survival remains unclear. The purpose of this study was to identify the prognostic biomarkers of locally advanced EC patients receiving neo-CRT.MethodsWe reviewed the data of 72 patients with cT2-4N0-3M0 EC who underwent neo-CRT at our hospital. The patients received intensity-modulated radiation therapy with a total radiation dose of 41.4–60.0 Gy. Most patients received platinum + paclitaxel-based combination regimens every three weeks for 2–4 cycles. The recorded data included age, sex, smoking history, alcohol use, histology, tumor location, clinical TNM stage, tumor length, gross tumor volume (GTV), GTV of primary tumor (GTVp), GTV of lymph nodes (GTVn), radiation dose, and number of chemotherapy cycles. Overall survival (OS), progression-free survival (PFS), and pathological complete response (pCR) were analyzed.ResultsThe 3-year OS and PFS rates of these patients who underwent neo-CRT were 51.14% and 43.28%, respectively. In the univariate analyses, smoking history, clinical stage, GTV, GTVp, and GTVn were significantly associated with OS, whereas alcohol use, GTV, GTVp, and GTVn were significantly associated with PFS. Furthermore, in the multivariate analysis, GTV was an independent prognostic predictor of OS (hazard ratio (HR): 14.14, 95% confidence interval (CI): 3.747–53.33, P < 0.0001) and PFS (HR: 6.090, 95% CI: 2.398–15.47, P < 0.0001). In addition, GTV < 60.50 cm3 compared to > 60.50 cm3 was significantly associated with higher pCR rate (59.3% and 27.8%, respectively, P = 0.038). High dose (> 50 Gy) and increased number of chemotherapy cycles (≥ 3) didn’t improve the OS or PFS in patients with GTV > 60.50 cm3.ConclusionGTV was an independent prognostic factor of long-term survival in EC patients, which may be because GTV is associated with histological response to neo-CRT. Additionally, patients with GTV > 60.50 cm3 didn’t benefit from increased radiation dose or increased number of chemotherapy cycles.

Salt damage is an important abiotic stress affecting the agronomic traits of soybean. Soybeans rapidly sense and transmit adverse signals when salt-damaged, inducing a set of response mechanisms to resist salt stress. AtARA6 encodes a small GTPase, which plays an important role in Arabidopsis vesicle transport and salt tolerance. In this study, we transformed the Arabidopsis gene AtARA6 into the cultivated soybean Shen Nong 9 (SN9). To investigate the salt tolerance pathways affected by AtARA6 in soybean, we performed transcriptome sequencing using transgenic soybean and wild-type (SN9) under salt treatment and water treatment. Our results suggest that AtARA6 is involved in the regulation of soybean SNARE complexes in the vesicle transport pathway, which may directly strengthen salt tolerance. In addition, we comprehensively analyzed the RNA-seq data of transgenic soybean and SN9 under different treatments and obtained 935 DEGs. GO analysis showed that these DEGs were significantly enriched in transcription factor activity, sequence-specific DNA binding, and the inositol catabolic process. Three salt-responsive negative regulator transcription factors, namely MYC2, WRKY6, and WRKY86, were found to be significantly downregulated after salt treatment in transgenic soybeans. Moreover, four genes encoding inositol oxygenase were significantly enriched in the inositol catabolic process pathway, which could improve the salt tolerance of transgenic soybeans by reducing their reactive oxygen species content. These are unique salt tolerance effects produced by transgenic soybeans. Our results provide basic insights into the function of AtARA6 in soybeans and its role in abiotic stress processes in plants.

Abstract Background The incidence of nasopharyngeal carcinoma (NPC) is rare, but a certain amount of mortality remains in NPC patients. Our study aimed to identify candidate genes as biomarkers for NPC screening, diagnosis, and therapy. Methods We investigated two microarray profile datasets GSE64634 and GSE12452 to screen the potential differentially expressed genes (DEGs) in NPC. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the DEGs were also performed. A protein-protein interaction (PPI) network of DEGs was constructed by STRING and visualized by Cytoscape software. The associated transcriptional factor regulatory network of the DEGs was also constructed. Results A total of 152 DEGs were identified from the GSE64634 and GSE12452 datasets, including 10 upregulated and 142 downregulated genes. Gene functional enrichment analysis indicated that these DEGs were enriched in the cilium movement, antimicrobial humoral response, O-glycan processing, mucosal immune response, carbohydrate transmembrane transporter activity, hormone biosynthetic process, neurotransmitter biosynthetic process, and drug metabolism-cytochrome P450 pathway. Five hub genes (DNALI1, RSPH4A, RSPH9, DNAI2, and ALDH3A1) and one significant module (score = 5.6) were obtained from the PPI network. Key transcriptional factors, such as SPI1, SIN3B, and GATA2, were identified with close interactions with these five hub DEGs from the gene-transcriptional factor network. Conclusions With the integrated bioinformatic analysis, numerous DEGs related to NPC were screened, and the hub DEGs we identified may be potential biomarkers for NPC.

Ground-based oblique incidence sounding (OIS) is an important means to investigate the ionosphere. As the OIS ionogram is a visual representation of the OIS parameters, such as group distance and maximum usable frequency (MUF), it is of great significance for improving the quality and the range resolution. This will facilitate the automatic interpretation and inversion of OIS ionograms to obtain the fine structure and spatial–temporal evolutions of the ionosphere. In this paper, a novel OIS signal processing scheme is proposed based on the Eigenspace-based (ESB) beamforming technology and Capon high-resolution range profile (HRRP). First, by applying the ESB beamformer to a compact L-shaped antenna array, the energy of the OIS signals received by multiple antennas can be added, while the interference and noise will be suppressed. Subsequently, the Capon HRRP algorithm is used to improve the range resolution. This is achieved due to the slow variation in the characteristics of the ionosphere resulting in good short-term coherence of the narrowband signals. The experimental results show that the two-stage signal-processing method significantly improves the imaging quality of OIS ionograms. In particular, the structure inside the ionosphere and its temporal and spatial evolution can be observed more precisely after the range resolution of the OIS ionogram is improved; therefore, it has great application potential.

In this paper, we explored how to more accurately predict the quality of high-frequency links and how to better research and improve the capabilities of high-frequency communication, reconnaissance, and positioning systems. Based on the background electron density generated by the ionospheric assimilation model and 3D ray-tracing technology, more realistic and accurate high-frequency channel parameters with physical meanings were obtained. On this basis, a complete high-frequency channel model that can be used for simulation and prediction was constructed. First, the ionospheric assimilation model, the high-frequency channel model, and the method used for calculating the parameters of the high-frequency channel model based on the background electron density generated by the multi-source ionospheric assimilation model are introduced. Then, the HF oblique sounding experiment and experimental data processing are introduced. Finally, the modeling and simulation of the high-frequency channel are compared with the HF oblique sounding experimental results. The simulation results showed that the modeling results of the high-frequency channel based on the multi-source ionospheric assimilation model proposed in this paper were similar to the HF oblique sounding experimental results. The average deviation of the difference between the simulation results and the experimental ones of the group path, the group path broadening, and the Doppler frequency shift are 29.2200 km, 17.3456 km, and 0.2121 Hz, respectively. The group delay, Doppler frequency shift, and delay broadening results calculated by the high-frequency channel model simulation were relatively accurate and could be used in high-frequency channel quality reporting and prediction, high-frequency reconnaissance and geolocation, and high-frequency radar frequency selection and positioning, etc.

The lower atmosphere forcing plays an important role in forming the sporadic E (Es) layer in the ionosphere. In this study, a comparative study of the Es layer recorded by ionosondes at the middle latitude regions was carried out between the plateau and plain regions in China. The two ionosonde stations (Zhangye, 39.21°N, 100.54°E and Beijing, 40.25°N, 116.25°E) are located at the Qinghai–Tibet Plateau and North China Plain, respectively. The data during the year 2018 were used to reveal the characteristics of the Es layer. The occurrence probability, the critical frequency (foEs) and the base virtual height (h’Es) were considered in this study. Results show that: (1) The diurnal and seasonal variations of the occurrence probability between these two regions are similar. The maximum occurrence probability is at noontime and in the summer season. However, the Es at Zhangye occurred more frequently than Beijing at nighttime and in winter to early spring. (2) Similar to previous studies, the maximum value of foEs at Beijing mainly occurred in summer. Interestingly, the maximum value is in winter at Zhangye station. (3) The characteristics of the anomaly of the Es layer at Zhangye are mostly consistent with the characteristics of atmospheric gravity waves in the Qinghai–Tibet Plateau. Therefore, compared with observations at Beijing, the anomalies of the Es layer at Zhangye (at night and in winter to spring) might be attributed to gravity waves in the lower atmosphere over the Qinghai–Tibet Plateau.

The sporadic E (Es) layer is a thin layer of ion plasma enhancement in the E-region ionosphere, typically at altitudes of 90–120 km with vertical and horizontal extent of several or several tens of kilometers. As the transition region between the lower and upper atmosphere, this layer is of critical importance for ionospheric studies. The most economical but effective method to observe this layer is using ionosonde, which, however, is incapable of capturing the finer structure or the internal inhomogeneity of the Es layer as the range resolution is on the order of kilometers. To overcome this limitation, we employ the frequency domain interferometry (FDI) technique, a technique that has been successfully applied to the analysis of some radar and sonar measurements. Here, we use the Es layer measurements near Wuhan, China (114°22′E, 30°30′N) on 8 June 2021 as examples to showcase the capability of this technique. Our results show that the spatial resolution of ionosonde imaging is remarkably increased: the complexity of the internal fine structure in the Es layer can be well observed in the FDI-processed ionograms, whereas the intrinsic range resolution is several kilometers. Moreover, by comparing the ionograms obtained with and without the FDI technique, it is found that the FDI-processed ionogram is particularly suitable for the observation of evolutional processes in the Es layer, as well as the identification of different types of Es layer. With this level of spatial resolution, ionosonde, in combination with the FDI technique, opens the possibility for more refined observations of the Es layer.

In this study, a method is proposed to carry out automatic inversion of oblique ionograms to extract the parameters and electron density profile of the ionosphere. The proposed method adopts the quasi-parabolic segments (QPS) model to represent the ionosphere. Firstly, numerous candidate electron density profiles and corresponding vertical traces were, respectively, calculated and synthesized by adjusting the parameters of the QPS model. Then, the candidate vertical traces were transformed to oblique traces by the secant theorem and Martyn’s equivalent path theorem. On the other hand, image processing technology and characteristics of oblique echoes were adopted to automatically scale the key parameters (the maximum observable frequency and minimum group path, etc.) from oblique ionograms. The synthesized oblique traces, whose parameters were close to autoscaled parameters, were selected as the candidate traces to produce a correlation with measured oblique ionograms. Lastly, the proposed algorithm searched the best-fit synthesized oblique trace by comparing the synthesized traces with oblique ionograms. To test its feasibility, oblique ionograms were automatically scaled by the proposed method and these autoscaled parameters were compared with manual scaling results. The preliminary results show that the accuracy of autoscaled maximum observable frequency and minimum group path of the ordinary trace of the F2 layer is, respectively, about 91.98% and 86.41%, which might be accurate enough for space weather specifications. It inspires us to improve the proposed method in future studies.

OBJECTIVE: This study was conducted to assess the efficacy and safety of nimotuzumab combined with radiotherapy (RT) in elderly patients with nasopharyngeal carcinoma. MATERIALS AND METHODS: The clinical data of 75 nasopharyngeal carcinoma patients, who were initially treated with nimotuzumab combined with RT, were collected and retrospectively reviewed from December 2008 to April 2014. They were aged 60 to 81 years (median 64 years). The distribution of disease was stage II in 10 (13.3%), stage III in 33 (44.0%), and stage IV in 32 (42.7%). Among these patients, 59 cases received cisplatin-based chemotherapy. Survival outcomes and treatment toxicity were analyzed using IBM SPSS 19.0 software. RESULTS: With a median follow-up of 45 months (range, 13-78 months), the estimated 3-year local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS), distant metastasis-free survival (DMFS), progression failure-free survival (PFS), and overall survival (OS) rates were 95.6%, 95.5%, 98.6%, 89.7%, and 89.2%, respectively. In the subgroup, 3-year OS rate in the patients with concurrent chemotherapy was 90.5% and 77.4% in patients without concurrent chemotherapy (Log-Rank = 1.795, P = .180). Univariate analysis showed that T stage and clinical stage were correlated with OS. Multivariate analysis indicated that age, T stage and tumor response at the end of treatment were independent prognosticators. Nine patients experienced grade 3 to 4 acute mucositis and 26 patients experienced grade 3-4 leukocytopenia, with no cases of skin rash and infusion reaction. Twelve patients developed mild liver function damage. No serious gastrointestinal or renal toxicities were observed. CONCLUSION: The efficacy of combined nimotuzumab with RT in elderly NPC patients was encouraging and the toxicities were accepted. In addition, nimotuzumab provides a better option for elderly patients who cannot be tolerate chemotherapy.

OBJECTIVES: To report the long-term outcome and toxicity of locoregionally advanced nasopharyngeal carcinoma (LA NPC) treated with nimotuzumab (h-R3) plus intensity-modulated radiotherapy (IMRT) with or without chemotherapy. METHODS: From May 2008 to March 2014, 3022 newly histology-proven, nonmetastatic NPC patients were retrospectively reviewed; among them, 257 patients treated with h-R3 were enrolled in this study. The patients' age range was between 10 and 76 years. The distribution of patients by disease stage was 150 (58.4%) in stage III, 88 (34.2%) in stage IV A, and 19 (7.4%) in stage IV B. All the patients received the treatment of h-R3 plus IMRT, and from them, 239 cases were also treated with cisplatin-based chemotherapy. Acute and late radiation-related toxicities were graded according to the Acute and Late Radiation Morbidity Scoring Criteria of Radiation Therapy Oncology Group. The accumulated survival was calculated according to the Kaplan-Meier method. Log-rank test was used to compare the survival difference. Multivariate analysis was performed using Cox's proportional-hazard model. RESULTS: All 257 patients had completed combined treatment; 231 patients received h-R3 plus IMRT with induction chemotherapy (IC), while 26 patients received only h-R3 plus IMRT. With a median follow-up of 48 months (range, 13-75 months), the estimated 5-year local recurrence-free survival, regional recurrence-free survival, distant metastases-free survival, progression-free survival, and overall survival (OS) rates were 94.3%, 94.8%, 91.9%, 83.4%, and 86.2%, respectively. Univariate analysis showed that age, T stage, clinical stage, and IC were related with OS. Multivariate analysis indicated that T stage and IC were independent prognostic factors for OS. The incidence of grade 3 to 4 acute mucositis and leukocytopenia was 10.9% and 19.8%, respectively, with no cases of skin rash and infusion reaction. Xerostomia was the most common late complication, and the degree of dry mouth in most survivors was mild to moderate at the last follow-up time. CONCLUSION: h-R3 plus IMRT with or without chemotherapy showed promising outcomes in terms of locoregional control and survival without increasing the incidence of radiation-related toxicities for patients.

This paper describes a novel sounding system for which the functions of the medium frequency (MF) radar and the ionosonde are integrated on the same hardware platform and antenna structure, namely the middle atmosphere-ionosphere (MAI) system. Unlike the common MF radar, MAI system adopts the pseudo-random (PRN) phase-coded modulation technology, which breaks the limitation of the traditional monopulse mode. Through the pulse compression, only a small peak power is needed to achieve the signal-to-noise ratio (SNR) requirement. The excellent anti-jamming performance is also very suitable for the ionospheric sounding. One transmitting and six receiving modes are adopted for the MF sounding. While neglecting the structure of the T/R switches, the coupling interference between the transmitter and the receiver may also be avoided. Moreover, by employing a miniaturized antenna array composed of progressive-wave antennas for the MF receiving and ionospheric sounding, the MAI system takes account of the requirements of the inversion algorithms of MF radar and the large bandwidth need for the ionospheric sounding concurrently. Such an antenna structure can also greatly simplify the system structure and minimize the difficulty of deployment. The experiments verified the availability of the system scheme and its engineering application significance. Through further analysis of the sounding data, the wind field of the mesosphere, the electron density of D layer and electron density profile from layers E to F were obtained at the identical location. The capability of MAI system can play an important role in studying the interaction and coupling mechanism between the mesosphere and ionosphere.

In this paper, a new dedicated multi-channel ionospheric oblique backscatter sounder is described. A miniaturized L-shaped antenna array was employed for the receiving of the oblique backscatter echoes in the present system. Firstly, two typical adaptive beamforming algorithms were introduced to improve the anti-jamming ability. Then, simulations were carried out to verify the beamforming performance in azimuth and elevation simultaneously. Furthermore, the experimental results by the present sounding system were used to test the performance of the adaptive beamformers. Results show that the radio frequency interference and the interference of the vertical echoes can be effectively suppressed by the adaptive beamformers. In particular, the use value of the beamforming in the receiving of the ionospheric oblique backscatter sounding is described in detail through the analysis of the signal sequences in several typical frequencies. And after the constant false-alarm rate processing, the oblique backscatter ionograms processed by the adaptive beamforers have clearer and continuous leading edge compared with the original ionograms. As a result, the adaptive beamformers of great significance to improve the detection ability of the ionospheric oblique backscatter sounding.

In this paper, complete complementary code (CCC) sequences are applied to a High Frequency (HF) ionospheric sounding network. Ionosondes distributed at multiple locations use the mutually orthogonal CCC sequences to conduct vertical soundings synchronously. At the same time, thanks to the omnidirectional antennas, every station can receive the oblique echoes transmitted from the others. Due to the orthogonality between the code sequences, both vertical and oblique ionograms can be simultaneously obtained and completely separated. Through this method, the sounding efficiency can be enhanced, and the inversion difficulty can be reduced. Further, by using the data assimilation method, vertical and oblique sounding results can be combined to obtain a wide range of regional ionospheric characteristics. To verify the performance of this kind of sounding network, validation experiments are implemented to demonstrate that vertical and oblique ionograms can be obtained independently at the same time and inverted separately and that the maps of foF2 parameters obtained by using the data assimilation method provide more details than single vertical or oblique sounding.

This paper illustrates the processes carried out for the application of biphase complete complementary code (CCC) for ionospheric sounding to address the coherent interference problem in multi-station ionospheric sounding. An algorithm to generate the biphase CCC is described, and the detailed process of waveform construction and signal processing is presented. Characteristics of the autocorrelation and cross-correlation are analyzed through simulations, and the technical feasibility of the application of CCC is explored. Experiments of ionospheric sounding with the CCC are also implemented to verify performance. Results demonstrate that the CCC performs well in multi-station ionospheric sounding, and is capable of eliminating the coherent interference in the network of ionosondes, compared to the conventional complementary code.

The expression of oviduct-specific recombinant proteins in transgenic chickens is a promising technology for the production of therapeutic biologics in eggs. In this study, we constructed a lentiviral vector encoding an expression cassette for human neutrophil defensin 4 (HNP4), a compound that displays high activity against Escherichia coli, and produced transgenic chickens that expressed the recombinant HNP4 protein in egg whites. After the antimicrobial activity of the recombinant HNP4 protein was tested at the cellular level, a 2.8-kb ovalbumin promoter was used to drive HNP4 expression specifically in oviduct tissues. From 669 injected eggs, 218 chickens were successfully hatched. Ten G0 roosters, with semens identified as positive for the transgene, were mated with wild-type hens to generate G1 chickens. From 1,274 total offspring, fifteen G1 transgenic chickens were positive for the transgene, which was confirmed by PCR and Southern blotting. The results of the Southern blotting and genome walking indicated that a single copy of the HNP4 gene was integrated into chromosomes 1, 2, 3, 4, 6 and 24 of the chickens. As expected, HNP4 expression was restricted to the oviduct tissues, and the levels of both transcriptional and translational HNP4 expression varied greatly in transgenic chickens with different transgene insertion sites. The amount of HNP4 protein expressed in the eggs of G1 and G2 heterozygous transgenic chickens ranged from 1.65 μg/ml to 10.18 μg/ml. These results indicated that the production of transgenic chickens that expressed HNP4 protein in egg whites was successful.

Transgenic chickens as bioreactors have several advantages, such as the simple establishment procedure, correct glycosylation profile of expressed proteins, etc. Lysozyme is widely used in food industry, livestock farming, and medical field as a replacement of antibiotics because of its antibacterial and complement system-modulating activity. In this study, we used RT-PCR, Western blot, and immunofluorescence to detect the expression of recombinant human lysozyme (rhLY) in the transgenic chicken. We demonstrated that the transgene of rhLY was genetically stable across different generations. We next optimized the purification procedure of rhLY from the transgenic eggs by utilizing two steps of cation-exchange chromatography and one gel-filtration chromatography. About 6 mg rhLY with the purity exceeding 90% was obtained from ten eggs, and the purification efficiency was about 75%. The purified rhLY had similar physicochemical and biological properties in molecular mass and antibacterial activity compared to the commercial human lysozyme. Additionally, both of them exhibited thermal stability at 60°C and tolerated an extensive pH range of 2 to 11. In conclusion, our study proved that the transgenic chickens we have previously generated were genetically stable and suitable for the production of active rhLY. We also provided a pipeline for purifying the recombinant proteins from transgenic eggs, which could be useful for other studies.

Chicken egg lysozyme (cLY) is an enzyme with 129 amino acid (AA) residue enzyme. This enzyme is present not only in chicken egg white but also in mucosal secretions such as saliva and tears. The antibacterial properties of egg white can be attributed to the presence of lysozyme, which is used as an anti-cancer drug and for the treatment of human immunodeficiency virus (HIV) infection. In this study, we constructed a lentiviral vector containing a synthetic cLY signal peptide and a 447 bp synthetic human lysozyme (hLY) cDNA sequence driven by an oviduct-specific ovalbumin promoter, and microinjected into the subgerminal cavity of stage X chick embryos to generate transgenic chicken. The transgene inserted in the chicken chromosomes directs the synthesis and secretion of hLY which has three times higher specific activity than cLY. Three G1 transgenic chickens were identified, the only female of which expressed recombinant human lysozyme (rhLY) at 57.66 ± 4.10 μg/ml in the egg white and the G2 transgenic hens of the G1 transgenic cock A011 expressed rhLY at 48.72 ± 1.54 μg/ml. This experiment demonstrated that transgenic hens with stable oviduct-specific expression of recombinant human lysozyme proteins can be created by microinjection of lentiviral vectors. The results of this research could be contribute to the technological development using transgenic hens as a cost-effective alternative to other mammalian systems, such as cow, sheep and goats, for the production of therapeutic proteins and other applications.

Silky-feather has been selected and fixed in some breeds due to its unique appearance. This phenotype is caused by a single recessive gene (hookless, h). Here we map the silky-feather locus to chromosome 3 by linkage analysis and subsequently fine-map it to an 18.9 kb interval using the identical by descent (IBD) method. Further analysis reveals that a C to G transversion located upstream of the prenyl (decaprenyl) diphosphate synthase, subunit 2 (PDSS2) gene is causing silky-feather. All silky-feather birds are homozygous for the G allele. The silky-feather mutation significantly decreases the expression of PDSS2 during feather development in vivo. Consistent with the regulatory effect, the C to G transversion is shown to remarkably reduce PDSS2 promoter activity in vitro. We report a new example of feather structure variation associated with a spontaneous mutation and provide new insight into the PDSS2 function.

Hepatitis C Virus (HCV) is a major public health concern, with no effective vaccines currently available and 3% of the world's population being infected. Despite the existence of both B- and T-cell immunity in HCV-infected patients, chronic viral infection and HCV-related malignancies progress. Here we report the identification of a novel HCV TCR from an HLA-A2-restricted, HCV NS3:1073-1081-reactive CTL clone isolated from a patient with chronic HCV infection. We characterized this HCV TCR by expressing it in human T cells and analyzed the function of the resulting HCV TCR-transduced cells. Our results indicate that both the HCV TCR-transduced CD4(+) and CD8(+) T cells recognized the HCV NS3:1073-1081 peptide-loaded targets and HCV(+) hepatocellular carcinoma cells (HCC) in a polyfunctional manner with cytokine (IFN-gamma, IL-2, and TNF-alpha) production as well as cytotoxicity. Tumor cell recognition by HCV TCR transduced CD8(-) Jurkat cells and CD4(+) PBL-derived T cells indicated this TCR was CD8-independent, a property consistent with other high affinity TCRs. HCV TCR-transduced T cells may be promising for the treatment of patients with chronic HCV infections.