Search

Tooth development, or odontogenesis, is a complex process in which several molecular pathways play a key role. Recently, microRNAs, a class of approximately 20-nucleotide small RNA molecules that regulate gene expression, have been implicated in the odontogenesis process. This study aimed to assess the role of miRNAs in odontogenesis anomalies, specifically agenesis and impaction. We analyzed a manually curated list of 82 miRNAs associated with human odontogenesis, sourced from literature data. Employing two different approaches to validate findings, we conducted functional enrichment analysis to evaluate the cell pathways, diseases, and phenotypes enriched for those miRNAs. Our findings indicate that the analyzed miRNAs regulate pathways linked to tooth anomalies, including the TGFꞵ and Wnt signaling pathways, and those governing the pluripotency of stem cells, known to mediate various cellular processes, and interconnected with odontogenesis-related pathways. Furthermore, the analysis disclosed several pathways associated with tumors, including small cell lung and gastric cancer. These results were confirmed also by diseases and phenotypes enrichment evaluation. Moreover, cell network analysis disclosed that miRNAs are embedded and interconnected in networks associated with dental diseases and cancer development, thus confirming the functional enrichment analyses. In summary, our results offer a quantitative measure of the potential involvement of miRNAs in regulating pathways crucial for developmental processes, notably odontogenesis, and provide results suggesting potential association with oncogenesis processes as well. [ABSTRACT FROM AUTHOR]

Introduction: The management of eruption disturbances in orthodontics may be challenging and requires a careful diagnosis and treatment planning. This case report discusses the challenges of a two-phase orthodontic treatment of a patient presenting with a dental eruption pattern anomaly. Patient concerns: A 10-year-old boy presented with no complaints for a routine orthodontic evaluation during the mixed dentition. Primary diagnoses: The patient was diagnosed with a skeletal Class I malocclusion with unilateral posterior crossbite, incomplete mandibular lateral incisor-canine transposition and a unilateral maxillary ectopic canine. Interventions: Phase 1 started with rapid maxillary expansion to correct the maxillary constriction and the ectopic eruption of the right maxillary canine. In the mandibular arch, phase 1 included the extraction of the left primary lateral incisor and canine, alignment of the left permanent lateral incisor and orthodontic traction of the left permanent canine. The duration of phase 1 was 14 months. Phase 2 involved a comprehensive course of orthodontic treatment and started when the patient was aged 13 years. This phase lasted 18 months. Results: An adequate dental occlusion was obtained, and the treatment results were stable after an 18-month follow-up. Conclusion: In this case, the early diagnosis of the dental anomalies was valuable as it allowed an early intervention to be undertaken, which resulted in overall treatment simplification and potentially minimised the adverse effects. This case report reinforces the importance of a careful follow-up during mixed dentition. [ABSTRACT FROM AUTHOR]

The objective of this article is to report the clinical case and 4-year follow-up of a 5-year-old child with multiple dental anomalies, emphasizing the importance of early diagnosis and use of combined pediatric surgery and orthodontic approaches. A 5-year-old boy, accompanied by his mother, sought dental care for dental caries and tooth pain. Clinical and radiographic examinations revealed active caries, a supernumerary primary tooth in the region of the mandibular right second premolar, and severe ankylosis of the primary mandibular right second molar. The treatment plan involved extraction of the supernumerary tooth as well as sectioning and extraction of the ankylosed molar. When the patient was 6 years old, the permanent mandibular right first molar showed signs of an altered eruptive process, and orthodontic treatment was initiated. A unilateral band-and-loop space maintainer with coil springs designed to move the permanent first molar was placed on the primary first molar. A new panoramic radiograph, obtained when the patient was aged 7 years, suggested the presence of an odontoma in the apical region of the primary maxillary right canine. Surgical removal and histopathologic examination of the lesion confirmed that it was a developing odontoma. After surgery, due to occlusal anomalies that included transverse maxillary deficiency, deep overbite, and midline deviation, the patient underwent rapid maxillary expansion therapy with a Haas-type appliance. When the patient was 8 years old, orthodontic treatment continued with a removable palatal Hawley expander and a fixed mandibular lingual arch. Currently, at the age of 9 years, the child is still undergoing fixed orthodontic treatment after surgical exposure of the impacted permanent maxillary right canine and bonding of an orthodontic attachment to enable traction. A multidisciplinary approach to the management of dental anomalies promotes a favorable prognosis and ensures comprehensive treatment of young patients. [ABSTRACT FROM AUTHOR]

Congenital infiltrating lipomatosis of the face (CILF) is a benign non-epithelial tumor that was initially reported in 1983. Although lipomas are generally encased in fibrous tissue, CILF lacks a fibrous capsule and is characterized by intramuscular invasion. We herein report a case of CILF diagnosed based on the early eruption of permanent teeth and reviewed the English literature. The patient was a 5-year-old male with diffuse swelling of the left upper canine gingiva. He had undergone surgery at 3 years old to debulk left buccal lipoma. At presentation, facial asymmetry and diffuse swelling of the left cheek were noted. Radiographic examinations revealed the early eruption of and accelerated root formation by the left upper permanent teeth, bony hyperplasia of the left maxilla and zygomatic bone, and adipose hyperplasia of the left cheek region. Hormone examinations revealed no abnormalities. A histopathological specimen obtained from surgery to reduce buccal lipoma was re-evaluated and diagnosed as infiltrative lipoma. Based on histopathological and clinical features, the patient was diagnosed with congenial infiltrative lipoma was established. Conservative therapy was performed in consideration of future surgical interventions after adolescence. [ABSTRACT FROM AUTHOR]

Background/purpose: Tooth development, or odontogenesis, is a complex process in which several molecular pathways play a key role. Recently, microRNAs, a class of approximately 20-nucleotide small RNA molecules that regulate gene expression, have been implicated in the odontogenesis process. This study aimed to assess the role of miRNAs in odontogenesis anomalies, specifically agenesis and impaction. Materials and methods: We analyzed a manually curated list of 82 miRNAs associated with human odontogenesis, sourced from literature data. Employing two different approaches to validate findings, we conducted functional enrichment analysis to evaluate the cell pathways, diseases, and phenotypes enriched for those miRNAs. Results: Our findings indicate that the analyzed miRNAs regulate pathways linked to tooth anomalies, including the TGFꞵ and Wnt signaling pathways, and those governing the pluripotency of stem cells, known to mediate various cellular processes, and interconnected with odontogenesis-related pathways. Furthermore, the analysis disclosed several pathways associated with tumors, including small cell lung and gastric cancer. These results were confirmed also by diseases and phenotypes enrichment evaluation. Moreover, cell network analysis disclosed that miRNAs are embedded and interconnected in networks associated with dental diseases and cancer development, thus confirming the functional enrichment analyses. Conclusion: In summary, our results offer a quantitative measure of the potential involvement of miRNAs in regulating pathways crucial for developmental processes, notably odontogenesis, and provide results suggesting potential association with oncogenesis processes as well.

Objective: To describe long-term outcomes and complications following mandibular distraction osteogenesis (MDO) in a diverse patient cohort Design: Cross-sectional study Setting: Single tertiary-care pediatric center Patients: Forty-eight patients previously undergoing MDO with minimum 4-year follow-up Main Outcome Measures: Respiratory outcomes, feeding patterns, dental development, motor/sensory nerve function, temporo-mandibular joint function, and postsurgical scarring Results: Forty-six patients with a median age of 7 years were evaluated. Of 20 nonsyndromic patients, none required additional airway procedures, none required continuous positive airway pressure (CPAP) during sleep, and 19 (95%) fed exclusively by mouth. Among 26 syndromic patients, 7 (27%) required CPAP and 8 (31%) were tube fed. Permanent first molar differences were seen in the majority of subjects; patterns of damage interfering with function were more common in syndromic (13/28, 46%) compared to nonsyndromic (5/24, 21%; P =.014) subjects. MDO prior to age two was associated with more frequent and worse dental damage (P =.001). Inferior alveolar nerve and marginal mandibular nerve function were fully intact in 37 (80%) and 39 (85%) of patients, respectively. Three patients (6%), all with associated genetic syndromes, demonstrated severe nerve impairment. By the Vancouver scar scale, ≥ 80% of surgical scars were rated in the most favorable category for each quality assessed. Temporomandibular joint dysfunction was rare. Conclusions: MDO shows highly favorable long-term respiratory, feeding, nerve, and scar outcomes in nonsyndromic patients, although permanent molar changes not precluding tooth viability are commonly seen. Patients with associated syndromes demonstrate respiratory and feeding benefits, but higher rates of dental and nerve abnormalities. [ABSTRACT FROM AUTHOR]

Abstract Background To investigate how successfully the classification of patients with and without dental anomalies was achieved through four experiments involving different dental anomalies. Methods Lateral cephalometric radiographs (LCRs) from 526 individuals aged between 14 and 22 years were included. Four experiments involving different dental anomalies were created. Experiment 1 included the total dental anomaly group and control group (CG). Experiment 2 only had dental agenesis and a CG. Experiment 3 consisted of only palatally impacted canines and the CG. Experiment 4 comprised patients with various dental defects (transposition, hypodontia, agenesis-palatally affected canine, peg-shaped laterally, hyperdontia) and the CG. Twelve sella measurements and assessments of the ponticulus posticus and posterior arch deficiency were given as input. The target was to distinguish between anomalies and controls. The CatBoost algorithm was applied to classify patients with and without dental anomalies. Results In order from lowest to highest, the predictive accuracies of the experiments were as follows: experiment 4

Background: To investigate how successfully the classification of patients with and without dental anomalies was achieved through four experiments involving different dental anomalies. Methods: Lateral cephalometric radiographs (LCRs) from 526 individuals aged between 14 and 22 years were included. Four experiments involving different dental anomalies were created. Experiment 1 included the total dental anomaly group and control group (CG). Experiment 2 only had dental agenesis and a CG. Experiment 3 consisted of only palatally impacted canines and the CG. Experiment 4 comprised patients with various dental defects (transposition, hypodontia, agenesis-palatally affected canine, peg-shaped laterally, hyperdontia) and the CG. Twelve sella measurements and assessments of the ponticulus posticus and posterior arch deficiency were given as input. The target was to distinguish between anomalies and controls. The CatBoost algorithm was applied to classify patients with and without dental anomalies. Results: In order from lowest to highest, the predictive accuracies of the experiments were as follows: experiment 4 < experiment 2 < experiment 3 < experiment 1. The sella area (SA) (mm2) was the most important variable in experiment 1. The most significant variable in prediction model of experiment 2 was sella height posterior (SHP) (mm). Sella area (SA) (mm2) was again the most relevant variable in experiment 3. The most important variable in experiment 4 was sella height median (SHM) (mm). Conclusions: Every prediction model from the four experiments prioritized different variables. These findings may suggest that related research should focus on specific traits from a diagnostic perspective. [ABSTRACT FROM AUTHOR]

Objective: To investigate the incidence of accessory canals and the variation in root canal morphology of permanent mandibular incisors and canines in Pakistani population using Cone Beam Computed Tomography (CBCT). Methods: A cross-sectional study was conducted in University College of Dentistry, The University of Lahore, Pakistan after getting institutional ethical permission from January 2020 to September 2022. The data included records of 111 patients consisting of 444 permanent mandibular incisors and 222 permanent mandibular canines. Accessory root, root canals and Vertucci canal configuration for each tooth was recorded. Data analysis was done using SPSS v20. Descriptive statistics were calculated for each anatomical parameter. Chi-square test was applied to determine association of gender with the presence of accessory roots and root canals. Results: Among the 111 records evaluated, 48.6% were males and 51.4% were females. No accessory root was found in the central and lateral incisors. However, an accessory root was found in 4.9% of the canines. The incidence of accessory canals in the central incisors, lateral incisors and canines was 18.9%, 25.2% and 10.4% respectively. The most common canal configuration in teeth with accessory canals was Type-III, followed by Type-II and Type-V. Conclusion: None of the central or lateral incisor showed accessory root while it was detected in 4.9% canines. The frequency of accessory root canal was found to be 18.9%, 25.2% and 10.4% in central incisors, lateral incisors and canines respectively. The most common canal configuration was Type-I, followed by Type-III and Type-II. Type-V, VI and VII were less common. [ABSTRACT FROM AUTHOR]

Copyright of Clinical Orthodontics is the property of Dental Press International and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Hearing impairments and dental anomalies are found in many genetic syndromes. Otodental syndrome is a rare combination of hearing loss and the presence of a pathognomonic dental phenotype known as globodontia, in which the tooth exhibits an abnormal globe shape. There is no histologic evidence of structural anomalies in the enamel, dentin, or pulp. This report describes the case of a 12-year-old boy who had hearing loss and 2 supernumerary globe-shaped teeth in the sites of the permanent maxillary central incisors. The diagnosis of otodental syndrome was established based on the clinical, radiographic, and histologic features, but other conditions, including dens evaginatus, talon cusp, dens invaginatus, and compound odontoma, should be included in the differential diagnosis. Dental treatment consisted of the extraction of both anomalous teeth, allowing spontaneous eruption of the impacted permanent central incisors. Early diagnosis of otodental syndrome permits a multidisciplinary approach to prevent other pathologic conditions, reduce functional damage, and avoid social problems, [ABSTRACT FROM AUTHOR]

Unique anatomical deviations in canal structure are rare in anterior teeth, especially central incisors, and thus risk being overlooked. For successful intervention, a meticulous diagnostic procedure and treatment plan, significantly aided by cone-beam computed tomography (CBCT), are crucial. The case at hand explores the management of a maxillary left central incisor in a cleft palate patient, characterized by multiple developmental lobes, a bulbous crown, and an atypical root anatomy. The primary symptom was pain, accompanied by a history of trauma at age 8 years and ensuing tooth discoloration. Initial evaluations, augmented by CBCT, revealed pulpal necrosis in a single-rooted tooth with three distinct canals. Initial clinical examination was supplemented by electrical pulp testing, RadioVisioGraphy (RVG), and CBCT, after which the root canal therapy was initiated. Informed consent was obtained from the patient. The access cavity preparation resulted in a three-orifice cavity. Subsequently, the canals were enlarged and sufficiently debrided. Calcium-hydroxide was applied for 2 weeks before the commencement of apexification and obturation, followed by esthetic rehabilitation. This case highlights the importance of recognizing rare anatomical variations in anterior teeth and demonstrates the invaluable role of CBCT in both diagnosing and managing such complexities. [ABSTRACT FROM AUTHOR]

Objectives: This study aims to provide insights into the developmental characteristics of the upper lateral incisor in individuals with unilateral clefts. Materials and methods: Panoramic radiographs of a consistent group of Caucasian children taken over time (ages 6, 9, and 12) were extensively reviewed. The study assessed the distribution pattern, eruption path, tooth development, and crown size of the upper lateral incisor within the cleft region. Results: The most commonly observed distribution pattern was the lateral incisor located distal to the cleft, accounting for 49.2% of cases. Furthermore, a significant delay in tooth development of the upper lateral incisor on the cleft side was noted at ages 6 and 9 (p > 0.001). Compared with the non-cleft side, these incisors often erupted along the alveolar cleft and exhibited microdontia (88.3%, p < 0.041). Conclusion: Lateral incisors on the cleft side display unique distribution patterns, microdontia, and delayed tooth development. Careful monitoring of the cuspid eruption is essential, as it can influence the eruption of the lateral incisor. Clinical relevance: A comprehensive understanding of the development of the upper lateral incisor relative to the cleft is vital for determining its prognosis over time. The position of the upper lateral incisor can also influence the timing and prognosis of secondary alveolar bone grafting. Preserving the upper lateral incisor favors arch length, perimeter, and symmetry in individuals with unilateral clefts. [ABSTRACT FROM AUTHOR]

Introduction and purpose: Hypodontia and hyperdontia are two common developmental dental anomalies characterized by an abnormal number of teeth and they present a significant challenge in dentistry, affecting both the functionality and aesthetics of the dentition. Hypodontia refers to the congenital absence of one or more teeth, while hyperdontia is characterized by the presence of supernumerary teeth in the oral cavity. These conditions can lead to various complications, such as bite issues, speech disorders, and difficulties in daily functioning. State of knowledge: Tooth number anomalies can have various causes, ranging from evolutionary reduction in dentition due to lifestyle changes over the years. The reduction in the number of teeth is currently among the most common developmental dental defects. Environmental factors also influence tooth number, acting as either local or general factors. Genetic influences should also be considered, as hypodontia and hyperdontia can be hereditary, and gender also plays a role in the predisposition to these conditions. Numerical anomalies in teeth can also be a symptom of certain genetically determined syndromes. Summary: Hypodontia and hyperdontia are examples of developmental disorders related to the number of teeth in the oral cavity, with various underlying causes. Diagnosis and subsequent treatment are crucial in managing these conditions. These disorders may require long-term therapies involving different areas of dentistry and interdisciplinary cooperation or may simply require ongoing clinical and radiological observation. Treatment depends on the severity of the disorder and the individual needs of the patient. However, thanks to advances in diagnosis and treatment, it is possible to effectively prevent complications.

Background: Amelogenesis Imperfecta (AI) is a disorder of tooth development characterized by abnormal enamel formation. In order to detect other dental and jawbone abnormalities that could be associated with AI, a retrospective and analytic study was conducted comparing panoramic radiographs of AI and non‐AI patients. Material and Methods: Digital panoramic radiographs of 60 AI and 60 non‐AI patients were examined. Abnormalities in dental number, size, shape, eruption, and in the shape of the dental arches were checked and blindly recorded by two experimented observers. Descriptive statistics using percentages and chi‐square test with.05 level of significance value was used. Results: Prevalence of supernumerary teeth, dental agenesis, microdontia, taurodontism, radicular dilacerations, dental inclusions, temporary teeth persistence, and pulp calcifications was significantly higher in AI patients compared to control patients. Prevalence of periapical images, cysts, and hypercementosis was lower in AI patients compared to control patients, with no statistically significant difference. A significant prevalence of mandibular hypoplasia was also noted in AI patients. Conclusion: In addition to enamel defect, panoramic radiography was useful in detecting other dental abnormalities and mandibular hypoplasia associated with AI and should therefore be systematically indicated for AI patients' care. [ABSTRACT FROM AUTHOR]

Objective: This study assesses the degree of root curvature in patients with non-syndromic cleft lip and/or palate (NSCL/P). Design: Retrospective. Case-control study. Setting: Root curvature was assessed in lower premolars and molars in 800 panoramic radiographs: 400 from patients with cleft and 400 from healthy control individuals. Root curvature was classified according to its angulation, as well as its apical, medial, or coronal localization. Results: The frequency of mild curvature in the NSCL/P group compared to the control group was higher in premolars especially in the left second premolar in cleft palate (OR: 6.91; 95% CI: 3.23−14.77; P <.0001). The frequency of moderate curvature in molars was significantly higher in the cleft group, with the highest risk in the right first molar in the cleft lip group (OR: 2.74; 95% CI: 1.67−4.52; P <.0001). Inclination was more frequently observed in the apical third of the root in the group with cleft, whereas for the control group, the curvature was more frequent in the medial third. In patients with cleft, the OR of curvature in the apical third was significant in premolars (left lower second premolar: Cleft lip, OR: 1.91; 95% CI: 1.04−3.52; P =.03; right lower second premolar: Cleft lip, OR: 1.91, 95% CI: 1.04−3.50; P =.03, cleft lip and palate, OR: 1.75; 95% CI: 1.12−2.73; P =.01). Conclusion: The results of the current study indicate differences in root curvature in patients with non-syndromic cleft lip and/or palate, which should be considered during the dental treatment planning of patients. [ABSTRACT FROM AUTHOR]

Supernumerary teeth (ST) are a prevalent multifactorial dental anomaly. Although most ST cases are idiopathic, multiple ST can be linked to genetic disorders and may appear in many syndromes. A healthy 8-year-old female had retained deciduous maxillary incisors, which was the parents' main concern. On clinical and radiographic examination, the patient had multiple unerupted ST. The patient underwent comprehensive dental treatment by multidisciplinary dental specialties. The case discussion underpins the importance of early diagnosis of ST and early treatment to ensure a favorable outcome and results. [ABSTRACT FROM AUTHOR]

Aim: To report a case of molar‐incisor‐malformation (MIM) in a 7‐year‐old boy with a history of congenital infantile fibrosarcoma of the upper chest, which was surgically treated, without adjuvant radio/chemotherapy. Material and Methods: The boy presented with mobility and pain on percussion of the permanent mandibular first molars. The radiographic features were suggestive of MIM. Both teeth were extracted and processed for conventional light microscopic examination and non‐decalcified ground sections. Results: The microscopic features confirmed the diagnosis of MIM. Regular follow‐up was recommended. Conclusions: Medical interventions during hospitalization for cancer treatment, other than chemo/radiotherapy, may cause MIM by adversely affecting the function of Hertwig's epithelial root sheath. Regular dental screening in paediatric cancer patients may reveal more MIM cases and help in elucidating its pathogenesis. [ABSTRACT FROM AUTHOR]

Objective The aim of this study was to assess the frequency of mandibular second premolar (MnP2) distoangulation among orthodontic patients and the angular changes after a longitudinal follow-up. Methods The sample was collected from the orthodontic records of 865 patients. The distoangulation group was composed of 42 patients (mean age 9.29 ± 1.24 years, 16 male, 26 female) with distoangulation of MnP2. The control group was composed of 32 patients (mean age 9.38 ± 1.10 years, 15 male, and 17 female) without distoangulation of MnP2. Panoramic radiographs taken in the mixed (T1) and the early permanent dentition (T2) were analyzed in both groups. Longitudinal angular changes (distal angle θ and premolar-molar angle γ), degree of root formation, second premolar depth, and prevalence of associated dental anomalies were analyzed. Intergroup comparison was performed with Mann–Whitney, t-tests, and chi-square tests (P  < 0.05). Results The prevalence of MnP2 distoangulation in the mixed dentition was 4.85%. The distoangulation group showed a smaller initial distal angle (59.34o ± 8.41) when compared to control group (79.88o ± 7.60). The spontaneous eruption of the MnP2 with distoangulation was observed in 76.57% of the sample. MnP2 distoangulation was significantly associated with agenesis of its antimere, small maxillary lateral incisors, and deciduous molar infraocclusion. Limitations Severe cases of MnP2 distoangulation were absent in this study. Conclusions The frequency of MnP2 distoangulation among orthodontic patients was 4.85%. Mild to moderate distoangulated Mnp2 spontaneously uprighted from the mixed to the permanent dentition. Small lateral incisors, second premolar agenesis, and infraocclusion of deciduous molar were frequently found in cases with MnP2 distoangulation. [ABSTRACT FROM AUTHOR]

Objectives: This study aims to determine the prevalence of tooth agenesis and supernumerary teeth and characterize both anomalies in a Portuguese pediatric population. Methods: Clinical records of patients attending Pediatric Dentistry appointments in the Faculty of Dental Medicine of the University of Porto were consulted. Appointments from September 2020 to December 2021 were analyzed. For each patient, sex and age were recorded, along with any relevant data in their clinical history. Statistical analysis of the results was conducted using chi-square tests with a significance level of 0.05. The sample included 144 patients. Results: This study found a prevalence for tooth agenesis of 20.83% (11.81% excluding the third molars) and a prevalence for supernumerary teeth of 1.39%. The prevalence of agenesis was 26.1% for males and 16% for females. No association was established in this regard (p>0.05). The most affected teeth were the third molars, followed by the second premolars. The mandible had 52.63% of agenesis cases, but no association was found (p>0.05). Two cases of supernumerary teeth were found, both in male patients and in the second quadrant. No differences were inferred regarding sex (p>0.05). Conclusions: The results of this study contributed to the existing data regarding the prevalence of dental anomalies of number within a Portuguese population.

Congenital diaphragmatic hernia (CDH) is a severe congenital anomaly that is often accompanied by other anomalies. Although the role of genetics in the pathogenesis of CDH has been established, only a small number of disease-associated genes have been identified. To further investigate the genetics of CDH, we analyzed de novo coding variants in 827 proband-parent trios and confirmed an overall significant enrichment of damaging de novo variants, especially in constrained genes. We identified LONP1 (lon peptidase 1, mitochondrial) and ALYREF (Aly/REF export factor) as candidate CDH-associated genes on the basis of de novo variants at a false discovery rate below 0.05. We also performed ultra-rare variant association analyses in 748 affected individuals and 11,220 ancestry-matched population control individuals and identified LONP1 as a risk gene contributing to CDH through both de novo and ultra-rare inherited largely heterozygous variants clustered in the core of the domains and segregating with CDH in affected familial individuals. Approximately 3% of our CDH cohort who are heterozygous with ultra-rare predicted damaging variants in LONP1 have a range of clinical phenotypes, including other anomalies in some individuals and higher mortality and requirement for extracorporeal membrane oxygenation. Mice with lung epithelium-specific deletion of Lonp1 die immediately after birth, most likely because of the observed severe reduction of lung growth, a known contributor to the high mortality in humans. Our findings of both de novo and inherited rare variants in the same gene may have implications in the design and analysis for other genetic studies of congenital anomalies.

Simple Summary: Despite the multidrug nature of anticancer treatment, attempts are still being made to determine the relationship between the duration of chemotherapy, the dose of individual drugs, and the occurrence of dental developmental abnormalities. There are relatively few papers devoted to this issue, and all of them are based on the study groups composed of individuals receiving various treatment regimens. The current study includes a group of acute lymphoblastic leukemia survivors who underwent chemotherapy according to the ALL IC-BFM 2002 protocol. Contrary to the observations of some authors, the results of the current research suggest that the age at the start of chemotherapy is likely to be the strongest risk factor for toxic injury during tooth development. A small study cohort is a major limitation, but an evaluation of similar relationships at larger research centers would be helpful in better understanding the problem. The multidrug nature of anticancer treatment and different treatment protocols used in the studies are likely to be a major limitation in establishing real risk factors determining the occurrence of dental abnormalities. The authors aimed to establish a relationship between the duration and the dose of chemotherapy and the number of tooth adverse effects in the group receiving the same treatment. Of the 40 anticancer therapy recipients who attended the outpatient dental clinic, 7 leukemia survivors receiving the treatment according to the ALL IC-BFM 2002 protocol were selected. The study group consisted of four females and three males aged 92 to 207 months at the time of dental examination and 29 to 91 months at leukemia diagnosis. As a result of the clinical and radiological examination, dental abnormalities such as agenesis, tooth size reduction, root abnormalities, and taurodontia were identified, and the medical records of all survivors were reviewed in terms of drugs administered, their doses, and treatment schedules. No correlation was observed between the treatment duration of an intensive therapy, the entire therapy, and the number of tooth abnormalities. No relationship was also found between the number of dental abnormalities and the cumulative dose of vincristine, L-asparaginase, methotrexate, cyclophosphamide, cytarabine, and 6-mercaptopurine. The age at the onset of antineoplastic therapy is likely to be the strongest risk factor for toxic injury during tooth development. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Management of supernumerary teeth fused to the labial surface of permanent maxillary central incisors would require a multidisciplinary approach comprising of endodontic treatment, periodontal recontouring, and cosmetic composite restoration. The reported cases of supernumerary teeth fused to the labial surface of maxillary central incisors are rare. Such cases need multidisciplinary approaches. Herein, management of a supernumerary tooth fused to the labial surface of a maxillary central incisor is reported. Due to the presence of a communication path between the root canal systems of the two fused teeth, root canal therapy was performed first for the maxillary left central incisor and the supernumerary tooth. The crown of the supernumerary tooth was then removed in a surgical setting while preserving the root to maintain the thin covering of alveolar bone and prevent future periodontal problems. Subsequently, an esthetic composite restoration was performed. [ABSTRACT FROM AUTHOR]

Introduction. Dens invaginatus is an infrequent developmental anomaly of teeth with an unknown etiology. The prevalence rate ranges from 0.25% to 10%. The most affected teeth are lateral maxillary incisors. Various classifications have been proposed to categorize the different types of dens in dente. However, the Oehlers classification, named after the dentist who first described it, is the most commonly used for this malformation. This classification has three primary types and was introduced in 1957. Case Report. A 17-yearold female patient appeared at the Dentistry Clinic of Vojvodina, Novi Sad with a lateral maxillary incisor exhibiting dens invaginatus (Oehlers type IIIA). The pulp was necrotic, accompanied by a large periradicular lesion. The patient had previously been prescribed penicillin antibiotics, and we initiated endodontic therapy. The patient returned for the scheduled check-up appointment with swelling in the area of the canine fossa above the upper left lateral incisor. In consultation with an oral surgeon, the tooth was extracted with purulent content. Four days after the extraction, the patient received a resin bonded bridge. Conclusion. The clinical significance and importance of dens in dente lie primarily in its potential to manifest with various symptomatic indications and its association with the occurrence and development of dental caries. It is crucial for clinicians and dental professionals to remain highly attentive, vigilant, and alert to the potential presence of dens in dente. This involves considering and acknowledging the wide array of diverse presentations and manifestations that this anomaly can exhibit. [ABSTRACT FROM AUTHOR]

Aim: Palatogingival groove (PGG) is a morphological defect that usually affects maxillary incisors and may be related to periodontal and endodontic disease. The aim of this study was to evaluate the prevalence, characteristics, and clinical implications of the PGG in a Brazilian population. Materials and Methods: A convenience sample with 169 consecutive participants was evaluated in a crosssectional study from September 2021 to May 2022. Upper anterior teeth were evaluated by a single examiner considering the presence of PGG, its characteristics and the presence of caries, periodontal and endodontic disease. Chi-square test and logistic regression were applied and P value was set at 5%. Results: PGG prevalence was 21.67% in individuals and 5.9% in teeth. A logistic regression did not reveal significant differences between sex [female: odds radio (OR) 1 and male: OR 0.53] and race (white: OR 1; black: OR 0.81; brown: OR 0.70). The highest prevalence was found in the upper lateral incisors (68.6%), in the cingulum (80.39%), with greater occurrence in the mid-palatal face of the tooth (39.21%). A high prevalence of bleeding on probing (83.8%) and caries (37.8%) was found in teeth with PGG, but no pulp alterations were found. Conclusions: PGG prevalence is high when the number of individuals is considered, and the tooth more frequently affected by PGG is the upper lateral incisor, with greater occurrence in the mid-palatal and cingulum face. In addition to incisors, canines also can be affected by PGG. Teeth with PGG may present high rates of biofilm accumulation, periodontal inflammation, and caries. [ABSTRACT FROM AUTHOR]

Key Clinical Message Management of supernumerary teeth fused to the labial surface of permanent maxillary central incisors would require a multidisciplinary approach comprising of endodontic treatment, periodontal recontouring, and cosmetic composite restoration. Abstract The reported cases of supernumerary teeth fused to the labial surface of maxillary central incisors are rare. Such cases need multidisciplinary approaches. Herein, management of a supernumerary tooth fused to the labial surface of a maxillary central incisor is reported. Due to the presence of a communication path between the root canal systems of the two fused teeth, root canal therapy was performed first for the maxillary left central incisor and the supernumerary tooth. The crown of the supernumerary tooth was then removed in a surgical setting while preserving the root to maintain the thin covering of alveolar bone and prevent future periodontal problems. Subsequently, an esthetic composite restoration was performed.

ABSTRACT Dilated odontoma is a rare dental developmental anomaly that belongs to a class od dens in dente, being rarely described in the posterior region of the mandible. To be describe a dilated odontoma in the lower third molar region. this study had a qualitative, transversal, descriptive and documentar approach, of the case report type. Developed in the Integrated Health Clinics of a University in the Extreme South of Santa Catarina, in the Dentistry Service. This article presets a case of dilated odontoma in a 32-year-old male patient. The patient had pain and swelling in the left posterior region of the mandible. The Crown of this element was clinically within the normal range, however the radiographic examination showed barrel-shaped root alteration in element 38 and expansion of the buccal and lingual cortical bone. An incisional biopsy of the anomaly whas performed and sent to anatomopathological examination. According to the clinical, radiographic and anatomopathological characteristics, the diagnosis of dilated odontoma was reached. Due to the aberrant root anatomy, complete removal was chosen. The diagnosis of this abnormality depeds on the clinical, radiographic and anatomoathological characteristics and its therapy varies according to the invagination. In cases of deep invagination, extraction is indicated.

Copyright of Salud Uninorte is the property of Fundacion Universidad del Norte and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Peg teeth are incisors which mesio-distal incisal widths are smaller than the cervical widths. The significant reduced size and format of those teeth are often related to a distal migration of the maxillary central incisors and maxillary anterior diastema. The presence of bilateral canine guidance is recommended to obtain a balanced occlusion, as it promotes a single contact between the maxillary and mandibular canines on the working side, with no other horizontal contacts. In aesthetic reconstructions, dental ceramics present high resistance and optical properties that resemble natural teeth. Objective: The aim of this study is to report an aesthetic and functional rehabilitation of peg teeth and establishment of canine guidance with ceramic fragments and veneers. Case report: The 25-year-old female patient complained about her "sharp" teeth. Clinical examination revealed the presence of peg-shaped maxillary lateral incisors, diastema between maxillary central incisors, and absence of canine guidance. After patients digital smile design and in-office tooth bleaching, a study cast and diagnostic wax-up were obtained. Following mock-up and patients approval, dental preparations and impressions were performed. Lithium disilicate ceramics were fabricated and tried in mouth. Veneers were then bonded, followed by occlusal adjustments, finishing and polishing. Conclusion: Thus, the final result was a natural smile, integrating aesthetics and function after 1-year follow-up. [ABSTRACT FROM AUTHOR]

Copyright of Monatsschrift Kinderheilkunde is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Infraocclusion of deciduous molars is a clinical disturbance that occurs during primary and mixed dentition and has some orthodontic implications. Infraoccluded teeth are believed to be potential sites of malocclusion, with a risk of tipping neighbouring teeth and losing space. This systematic review aims to analyse the management of primary molars infraocclusion and to provide updated guidelines. Methods: A literature search was performed using PubMed, Scopus, and Web of Science databases from 1 January 2017 to 28 November 2022. The inclusion criteria were: studies only on human subjects, open access studies, case reports, randomised trials, retrospective, observational studies, and English language. Results: A total of 372 publications were identified from the databases and a final number of nine studies were included in the review for qualitative analysis. Conclusion: Management of patients suffering from infraocclusion depends on the severity, age at diagnosis, and presence of succeeded premolars. Early diagnosis of infraoccluded primary elements is fundamental and cannot be postponed. Preservation of the primary molars may be a valid option with long-term stability if there is no or moderate primary molar infraocclusion, root resorption of less than half of the root, and no decay or restoration. [ABSTRACT FROM AUTHOR]

Molar incisor hypomineralization (MIH) is a qualitative disturbance of the enamel of the permanent molars and/or incisors. Its etiology is not clearly defined but is connected with different factors occurring before and after birth. It remains difficult to identify a single factor or group of factors, and the problem is further complicated by various overlapping mechanisms. In this study, we attempted to determine whether DNA methylation—an epigenetic mechanism—plays a key role in the etiology of MIH. We collected the epithelium of the oral mucosa from children with MIH and healthy individuals and analyzed its global DNA methylation level in each child using a 5-mC DNA ELISA kit after DNA isolation. There was no statistically significant difference between the global DNA methylation levels in the study and control groups. Then, we also analyzed the associations of the DNA methylation levels with different prenatal, perinatal, and postnatal factors, using appropriate statistical methods. Factors such as number of pregnancies, number of births, type of delivery, varicella infection (under 3 years old), and high fever (under 3 years old) were significantly important. This work can be seen as the first step towards further studies of the epigenetic background of the MIH etiology.

Abstract Hypertrichosis and dental anomalies may occur alone or in combination in the spectrum of many syndromes. To identify genetic entities characterized by hypertrichosis and dental anomalies, a search was performed in the Mendelian Inheritance in Man database with the terms “hypertrichosis” or “hirsutism” and “tooth” or “dental abnormalities.” Nondependent androgen metabolism disturbances were classified as hypertrichosis. Genetic entities with hypertrichosis and dental anomalies were included in the study. Additional searches were performed in the PubMed and Orphanet databases, when necessary, in order to include data from scientific articles. An integrative analysis of the genes associated with the identified syndromes was conducted using STRING to characterize biological processes, pathways, and interactive networks. The p-values were subjected to the false discovery rate for the correction of multiple tests. Thirty-nine syndromes were identified, and dental agenesis was the most frequent dental anomaly present in 41.02% (n = 16) of the syndromes. Causative genes were identified in 33 out of 39 genetic syndromes. Among them, 39 genes were identified, and 38 were analyzed by STRING, which showed 148 biological processes and three pathways that were statistically significant. The most significant biological processes were the disassembly of the nucleosome (GO:0006337, p = 1.09e-06), chromosomal organization (GO:0051276, p = 1.09e-06) and remodeling of the chromatin (GO: 0006338, p = 7.86e-06), and the pathways were hepatocellular carcinoma (hsa05225, p = 5.77e-05), thermogenesis (hsa04714, p = 0.00019), and cell cycle (hsa04110, p = 0.0433). Our results showed that the identification of hypertrichosis and dental anomalies may raise the suspicion of one of the thirty-nine syndromes with both phenotypes.

Hypertrichosis and dental anomalies may occur alone or in combination in the spectrum of many syndromes. To identify genetic entities characterized by hypertrichosis and dental anomalies, a search was performed in the Mendelian Inheritance in Man database with the terms "hypertrichosis" or "hirsutism" and "tooth" or "dental abnormalities." Nondependent androgen metabolism disturbances were classified as hypertrichosis. Genetic entities with hypertrichosis and dental anomalies were included in the study. Additional searches were performed in the PubMed and Orphanet databases, when necessary, in order to include data from scientific articles. An integrative analysis of the genes associated with the identified syndromes was conducted using STRING to characterize biological processes, pathways, and interactive networks. The p-values were subjected to the false discovery rate for the correction of multiple tests. Thirty-nine syndromes were identified, and dental agenesis was the most frequent dental anomaly present in 41.02% (n = 16) of the syndromes. Causative genes were identified in 33 out of 39 genetic syndromes. Among them, 39 genes were identified, and 38 were analyzed by STRING, which showed 148 biological processes and three pathways that were statistically significant. The most significant biological processes were the disassembly of the nucleosome (GO:0006337, p = 1.09e-06), chromosomal organization (GO:0051276, p = 1.09e-06) and remodeling of the chromatin (GO: 0006338, p = 7.86e-06), and the pathways were hepatocellular carcinoma (hsa05225, p = 5.77e-05), thermogenesis (hsa04714, p = 0.00019), and cell cycle (hsa04110, p = 0.0433). Our results showed that the identification of hypertrichosis and dental anomalies may raise the suspicion of one of the thirty-nine syndromes with both phenotypes. [ABSTRACT FROM AUTHOR]

Copyright of RGO: Revista Gaúcha de Odontologia is the property of RGO: Revista Gaucha de Odontologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: Dental number anomalies are a group of congenital developmental disorders divided into two groups supernumerary and missing teeth. This study was conducted to investigate the prevalence of numeric dental anomalies using panoramic images in patients referred to the Hamadan Dental Faculty. Methods: In this cross-sectional study, 2,197 panoramic radiographs of patients aged 6-49 years were evaluated. These anomalies are divided into two groups: 1) Supernumerary teeth, including Mesiodens, Distodens, and Peridens, and 2) Missing teeth, including Hypodontia, Oligodontia, and Anodontia. A Chi-square test was performed to assess the relationship between the anomalies. Data analysis was performed using SPSS 16, in which P-value < 0.05 was considered the statistical significance level. Results: Of 736 males (32.2%) and 1548 females (67.8%) in this study, 32 (4.3%) and 55 cases (3.8%) had supernumerary teeth, respectively. The prevalence of supernumerary teeth was 0.3%, 0.5%, and 0.6% in males and 0.2%, 1% and 1.2% in females for mesiodens, distodens, and peridens, respectively. Also, 243 males (10.6%) and 655 females (28.6%) had missing teeth anomalies. Hypodontia in the maxilla was the most common anomaly in both genders, while mesiodens was the least common. Conclusion: Hypodontia was the most common anomaly, followed by peridens; the least common anomaly was mesiodens. The prevalence of supernumerary teeth was greater in males, though the difference was not statistically significant. In comparison, females had a greater prevalence of missing teeth. [ABSTRACT FROM AUTHOR]

Aim: To determine the prevalence of dental development anomalies and type of influence on the smile of adolescent students. Method: This was a cross-sectional and analytical study carried out in two public (A1) and two private (A2) schools chosen by lot in the city of Parnaíba-Piauí. The sample calculation was based on the target population: number of people enrolled in public and private schools between 15 and 19 years, in the city of Parnaíba-PI, which totaled 6209 students in 2020, according to a survey carried out by the Brazilian Institute of Geography and Statistics -- IBGE. A questionnaire on epidemiological data and aesthetic self-perception of the smile was applied to 160 adolescents between 15 and 19 years old, from August 2020 to July 2021. The clinical examination was carried out under natural light, to check for the presence of anomaly(s) in the dental development. Students who presented only one pathology would be called group 1 (G1), those who presented two would be called group 2 (G2) and those who presented 3 or more would be called group 3 (G3). On the other hand, adolescents in whom no anomaly was evidenced would participate in the control group (CG), both in A1 and A2. Results: It was observed that 37.5% of the sample had only a type of dental anomaly, corresponding to 60 individuals. The most prevalent were enamel hypoplasia, fusion, transposition, agenesis, ectopic eruption, microdent and dens-in-dent. It was possible to verify a higher prevalence in the maxilla, private schools (76.6%) and females (86.6%). In 45% of adolescents with dental anomalies, embarrassment was observed when smiling. Conclusion: The prevalence was relatively high, highlighting the enamel hypoplasia, influencing the smile esthetics of a reasonable number of adolescents, whether for acquaintances, strangers or even for photographs. [ABSTRACT FROM AUTHOR]

Copyright of Odontología Pediátrica is the property of Asociacion Sociedad Peruana de Odontopediatria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista de Odontopediatría Latinoamericana is the property of Asociacion Latinoamericana de Odontopediatria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: To investigate oral and dental late effects in survivors of childhood brain tumors medulloblastoma (MB) and central nervous system supratentorial primitive neuroectodermal tumor (CNS-PNET).Methods: This cross-sectional study assessed oral and dental late effects in MB/CNS-PNET survivors treated before 20 years of age, and with a minimum of 2 years since treatment. Participants went through an oral and radiographic examination. We assessed oral status using the decayed-missing-filled index (DMFT), oral dryness, maximum mouth opening (MMO), fungal infection, and registration of dental developmental disturbances (DDD) in the form of hypodontia, microdontia, and enamel hypoplasia.Results: The 46 participants' mean age at enrolment was 27 ± 12.8 years and at treatment 8.5 ± 5.2 years, and the mean time since treatment was 18.9 ± 12 years. Over a third (35%) of survivors had reduced mouth opening (mean 29.3 ± 5.6 mm (range 16-35)). A significantly lower MMO was found in individuals treated ≤ 5 years compared to survivors treated > 5 years (p = 0.021). One or more DDD were registered in 30.4% of the survivors, with a significantly higher prevalence in individuals treated ≤ 5 years (p < 0.001). Hypodontia was the most prevalent type of DDD. There was no difference in DMFT score in relation to age at treatment. Oral dryness was not frequently reported or observed in these survivors.Conclusion: Survivors of childhood MB/CNS-PNET are at risk of oral and dental late effects including reduced mouth opening and DDD. The risk is highest in survivors treated before the age of 5. [ABSTRACT FROM AUTHOR]