1. CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope.
- Author
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Allard, Antoine, Liboz, Maxime, Crépin, Raphaël, Labdi, Sid, Maciejak, Olek, Malo, Michel, Campillo, Clément, and Lamour, Guillaume
- Subjects
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ATOMIC force microscopy , *TOPOGRAPHIC maps , *GRAPHICAL user interfaces , *ATOMIC force microscopes , *SOFTWARE development tools - Abstract
Atomic force microscopy (AFM) is the gold-standard technique to simultaneously map the morphology and viscoelastic properties of living cells. Although existing software tools, both open-source and from AFM manufacturers, can analyze cells individually, there is a growing need for fast and accessible codes to compile data from multiple cells into a single dataset. To address this, we present CellMAP, a user-friendly software tool that streamlines the batch-processing of AFM-derived topography and stiffness maps of living cells. Our analysis pipeline includes but is not limited to: flattening of the underlying substrate surface, filtering of outlier values, measurement of the cell surface and volume, and measurement of height and stiffness distributions. CellMAP can also generate a composite cell that reflects the height and stiffness properties of an entire cell population. [ABSTRACT FROM AUTHOR]
- Published
- 2025
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