6 results on '"Torcal Garcia, Guillem"'
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2. Carm1-arginine methylation of the transcription factor C/EBPα regulates transdifferentiation velocity
- Author
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Torcal Garcia, Guillem, Kowenz-Leutz, Elisabeth, TIAN, TIAN, Klonizakis, Antonis, Lerner, Jonathan, de Andres-Aguayo, Luisa, Vila-Casadesús, Maria, Peiró, Sandra, Institut Català de la Salut, [Torcal Garcia G, Klonizakis A, De Andres-Aguayo L] Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Universitat Pompeu Fabra (UPF), Barcelona, Spain. [Kowenz-Leutz E] Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany. [Tian TV, Vila Casadesus M] Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. Universitat Pompeu Fabra (UPF), Barcelona, Spain. Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Lerner J] Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States. [Peiro S] Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
- Subjects
Cell Physiological Phenomena::Cell Differentiation [PHENOMENA AND PROCESSES] ,Cell Physiological Phenomena::Cell Transdifferentiation [PHENOMENA AND PROCESSES] ,Cèl·lules B ,fenómenos químicos::fenómenos bioquímicos::alquilación::metilación [FENÓMENOS Y PROCESOS] ,Chemical Phenomena::Biochemical Phenomena::Alkylation::Methylation [PHENOMENA AND PROCESSES] ,Diferenciació cel·lular ,Ratolins ,fenómenos fisiológicos celulares::transdiferenciación celular [FENÓMENOS Y PROCESOS] ,Metilació ,Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice [ORGANISMS] ,Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones [ORGANISMOS] ,fenómenos fisiológicos celulares::diferenciación celular [FENÓMENOS Y PROCESOS] - Abstract
Developmental biology; Gene regulation; Transcription factor Biologia del desenvolupament; Regulació gènica; Factor de transcripció Biología del desarrollo; Regulación génica; Factor de transcripción Here, we describe how the speed of C/EBPα-induced B cell to macrophage transdifferentiation (BMT) can be regulated, using both mouse and human models. The identification of a mutant of C/EBPα (C/EBPαR35A) that greatly accelerates BMT helped to illuminate the mechanism. Thus, incoming C/EBPα binds to PU.1, an obligate partner expressed in B cells, leading to the release of PU.1 from B cell enhancers, chromatin closing and silencing of the B cell program. Released PU.1 redistributes to macrophage enhancers newly occupied by C/EBPα, causing chromatin opening and activation of macrophage genes. All these steps are accelerated by C/EBPαR35A, initiated by its increased affinity for PU.1. Wild-type C/EBPα is methylated by Carm1 at arginine 35 and the enzyme’s perturbations modulate BMT velocity as predicted from the observations with the mutant. Increasing the proportion of unmethylated C/EBPα in granulocyte/macrophage progenitors by inhibiting Carm1 biases the cell’s differentiation toward macrophages, suggesting that cell fate decision velocity and lineage directionality are closely linked processes. TG was supported by the Center for Genomic Regulation, Barcelona, the Spanish Ministry of Economy, Industry and Competitiveness, (Plan Estatal PID2019-109354GB-100), AGAUR (SGR 006713) and the 4D-Genome European Research Council Synergy grant. KSZ was supported by the NIH grant R01GM36477. We have used ChatGPT to improve parts of the text.
- Published
- 2023
3. The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling
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Plana-Carmona, Marcos, primary, Stik, Gregoire, additional, Bulteau, Romain, additional, Segura-Morales, Carolina, additional, Alcázar, Noelia, additional, Wyatt, Chris D.R., additional, Klonizakis, Antonios, additional, de Andrés-Aguayo, Luisa, additional, Gasnier, Maxime, additional, Tian, Tian V., additional, Torcal Garcia, Guillem, additional, Vila-Casadesús, Maria, additional, Plachta, Nicolas, additional, Serrano, Manuel, additional, Francesconi, Mirko, additional, and Graf, Thomas, additional
- Published
- 2022
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4. Methylation of a lineage-instructive transcription factor acts as a cell fate switch
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Torcal Garcia, Guillem, 1991, Graf, T. (Thomas), and Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut.
- Subjects
Adipogenesis ,Modificacions post-traduccionals ,Hematopoesi ,Adipogènesi ,Cell fate decisions ,Decisions d'identitat cel.lular ,Embryo development ,Desenvolupament embrionari ,Hematopoiesis ,Post-translational modifications - Abstract
Lineage-instructive transcription factors orchestrate cell fate decisions by regulating gene expression programs. However, how their function is regulated throughout development is poorly understood. C/EBPα is a transcription factor that has been shown to play a key role in several cell fate decisions of several tissues, including myelopoiesis, adipogenesis and early embryo development. The protein arginine methyltransferase Carm1 has also been described to play a role in cell specification during various developmental processes, but a connection to C/EBPα has not been established. In this Thesis, we investigated the link between the two proteins in cell fate decisions. We found that arginine 35 of C/EBPα is a key target of Carm1 methylation, and that this post-translational modification alters the biological properties of the factor and acts as a cell fate switch during myelopoiesis, adipogenesis and the first cell fate decision between pluripotency and trophectoderm in early embryos. Los factores de transcripción instructores de linaje orquestan decisiones de identidad celular a través de la regulación de programas de expresión génica. Sin embargo, cómo su función está regulada durante el desarrollo todavía se desconoce. C/EBPα es un factor de transcripción que juega un papel clave en varias decisiones de identidad celular, incluyendo mielopoiesis, adipogénesis y el desarrollo embrionario temprano. La metiltransferasa de argininas Carm1 también está involucrada en los mismos procesos, pero todavía no se ha descrito una posible conexión con C/EBPα. En esta Tesis, hemos investigado la conexión entre éstas dos proteínas en decisiones de identidad celular. Hemos descubierto que la arginina 35 de C/EBPα es un blanco clave para la metilación por Carm1, y está modificación postraduccional altera las propiedades biológicas del factor y actúa como un interruptor de identidad celular en mielopoiesis, adipogénesis y en la primera decisión entre pluripotencia y trofectodermo en el embrión.
- Published
- 2022
5. The transcription factor code: a beacon for histone methyltransferase docking
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Torcal Garcia, Guillem, primary and Graf, Thomas, additional
- Published
- 2021
- Full Text
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6. From research to rapid response: mass COVID-19 testing by volunteers at the Centre for Genomic Regulation
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Ghose, Ritobrata, primary, Aranguren-Ibáñez, Álvaro, additional, Arecco, Niccolò, additional, Balboa, Diego, additional, Bataller, Marc, additional, Beltran, Sergi, additional, Benisty, Hannah, additional, Bénard, Angèle, additional, Bernardo, Edgar, additional, Carbonell Sala, Sílvia, additional, Casals, Eloi, additional, Ciampi, Ludovica, additional, Condemi, Livia, additional, Corvó, Alberto, additional, Cosín-Tomás, Marta, additional, Cuenca-Ardura, Mirabai, additional, Duran Serrano, Juan Manuel, additional, Espejo Díaz, María Isabel, additional, Fernandez Callejo, Marcos, additional, Gañez-Zapater, Antoni, additional, Garcia-Castellanos, Raquel, additional, Garrido, Romina, additional, Henkin, Gil, additional, Hermoso Pulido, Toni, additional, Hernandez-Alias, Xavier, additional, Herrero Vicente, Jorge, additional, Ingham, Matthew, additional, Lim, Wei Ming, additional, Llonch, Sílvia, additional, Marmesat Bertoli, Elena, additional, Miguel-Escalada, Irene, additional, Montero-Blay, Ariadna, additional, Navarrete Hernández, Cristina, additional, Neguembor, Maria Victoria, additional, Ní Chárthaigh, Róisín-Ana, additional, Pardo-Lorente, Natalia, additional, Pascual-Reguant, Laura, additional, Pérez-Lluch, Sílvia, additional, Perza, Reyes, additional, Pesaresi, Martina, additional, Picó Amador, Daniel, additional, Pifarré, Paula, additional, Piscia, Davide, additional, Plana-Carmona, Marcos, additional, Ponomarenko, Julia, additional, Radusky, Leandro, additional, Rivero, Ezequiel, additional, Rogalska, Malgorzata, additional, Torcal Garcia, Guillem, additional, and Wojnacki, José, additional
- Published
- 2020
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