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Your search keyword '"Toriyama, Koji"' showing total 118 results
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118 results on '"Toriyama, Koji"'

4. Development of a DualEmission Laser-Induced Fluorescence (DELIF) Method for Long-Term Temperature Measurements.

Author

Toriyama, Koji, Funatani, Shumpei, and Tada, Shigeru

Subjects
*LASER-induced fluorescence, *BANDPASS filters, *RHODAMINE B, *FLUORESCENCE spectroscopy, *TEMPERATURE measurements
Abstract

The fluorescence intensity of fluorescent dyes typically employed in the dual-emission laser-induced fluorescence (DELIF) method gradually degrades as the excitation time increases, and the degradation rate depends on the type of fluorescent dye used. Therefore, the DELIF method is unsuitable for long-term temperature measurements. In this study, we focused on the fluorescence intensity ratio of a single fluorescent dye at two fluorescence wavelengths and developed a DELIF method for long-term temperature measurements based on this ratio. The fluorescence intensity characteristics of Fluorescein disodium and Rhodamine B, which are typically used in the DELIF method, in the temperature range of 10–60 °C were comprehensively investigated using two high-speed monochrome complementary metal-oxide semiconductor cameras and narrow bandpass filters. Interestingly, the ratio of the fluorescence intensity of each fluorescent dye at the peak emission wavelength of the fluorescence spectrum, λ, to the fluorescence intensity at wavelengths very close to the peak wavelength (λ ± 10 nm) was highly sensitive to temperature variations but not excitation time. Particularly, when Rhodamine B was used, the selection of the fluorescence intensity ratios at a wavelength combination of 589 and 600 nm enabled highly accurate temperature measurements with a temperature resolution of ≤0.042 °C. Moreover, the fluorescence intensity ratio varied negligibly throughout the excitation time of 180 min, with a measurement uncertainty (95% confidence interval) of 0.045 °C at 20 °C. The results demonstrate that the proposed DELIF method enables highly accurate long-term temperature measurements. [ABSTRACT FROM AUTHOR]

Published
2024
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32. Reinforce the antitumor activity of CD8+ T cells via glutamine restriction.

Author

Nabe, Shogo, Yamada, Takeshi, Suzuki, Junpei, Toriyama, Koji, Yasuoka, Toshiaki, Kuwahara, Makoto, Shiraishi, Atsushi, Takenaka, Katsuto, Yasukawa, Masaki, and Yamashita, Masakatsu

Abstract

The antitumor activity of activated CD8+ T cells in the tumor microenvironment seems to be limited due to their being metabolically unfit. This metabolic unfitness is closely associated with T‐cell exhaustion and impairment of memory formation, which are barriers to successful antitumor adoptive immunotherapy. We therefore assessed the role of glutamine metabolism in the antitumor activity of CD8+ T cells using a tumor‐inoculated mouse model. The adoptive transfer of tumor‐specific CD8+ T cells cultured under glutamine‐restricted (dGln) conditions or CD8+ T cells treated with specific inhibitors of glutamine metabolism efficiently eliminated tumors and led to better survival of tumor‐inoculated mice than with cells cultured under control (Ctrl) conditions. The decreased expression of PD‐1 and increased Ki67 positivity among tumor‐infiltrating CD8+ T cells cultured under dGln conditions suggested that the inhibition of glutamine metabolism prevents CD8+ T‐cell exhaustion in vivo. Furthermore, the transferred CD8+ T cells cultured under dGln conditions expanded more efficiently against secondary OVA stimulation than did CD8+ T cells under Ctrl conditions. We found that the expression of a pro‐survival factor and memory T cell‐related transcription factors was significantly higher in CD8+ T cells cultured under dGln conditions than in those cultured under Ctrl conditions. Given these findings, our study uncovered an important role of glutamine metabolism in the antitumor activity of CD8+ T cells. The novel adoptive transfer of tumor‐specific CD8+ T cells cultured in glutamine‐restricted conditions may be a promising approach to improve the efficacy of cell‐based adoptive immunotherapy. This manuscript uncovered an important role of glutamine metabolism in regulating antitumor activity of CD8+ T cells. Adoptive transfer of tumor‐specific CD8+ T cells cultured in glutamine‐restricted conditions improved the efficacy of CD8+ T cell‐based cancer adoptive immunotherapy. [ABSTRACT FROM AUTHOR]

Published
2018
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35. Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

Author

Toriyama, Koji, primary, Suzuki, Takashi, additional, Inoue, Tomoyuki, additional, Eguchi, Hiroshi, additional, Hoshi, Saichi, additional, Inoue, Yoshitsugu, additional, Aizawa, Hideki, additional, Miyoshi, Kazutomi, additional, Ohkubo, Michio, additional, Hiwatashi, Eiji, additional, Tachibana, Hiroshi, additional, and Ohashi, Yuichi, additional

Published
2015
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