18 results on '"Torrents, Jorgelina"'
Search Results
2. Relationship between pharmacokinetics of fluazuron and its efficacy for controlling Rhipicephalus microplus: A comprehensive evaluation of tick drug uptake
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Sarli, Macarena, Miró, María Victoria, Rossner, María Victoria, Gómez, Viviana, Torrents, Jorgelina, Nava, Santiago, and Lifschitz, Adrián
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- 2024
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3. Evaluation of the impact of successive acaricide treatments on resistance evolution in Rhipicephalus microplus populations: Monodrugs versus drug combinations
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Sarli, Macarena, Torrents, Jorgelina, Toffaletti, José R., Morel, Nicolás, and Nava, Santiago
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- 2023
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- View/download PDF
4. Two novel Ehrlichia (Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
- Author
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Tarragona, Evelina L., Flores, Fernando S., Lamattina, Daniela, Torrents, Jorgelina, Sebastian, Patrick S., and Nava, Santiago
- Published
- 2022
- Full Text
- View/download PDF
5. Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina
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Sarli, Macarena, Torrents, Jorgelina, Morel, Nicolás, and Nava, Santiago
- Published
- 2022
- Full Text
- View/download PDF
6. Spatiotemporal heterogeneity of Rhipicephalus microplus resistance to chemical acaricides at intra‐farm level: A case study using ivermectin.
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Morel, Nicolas, Torrents, Jorgelina, Sarli, Macarena, Rossner, Maria V., Lifschitz, Adrián L., and Nava, Santiago
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CHEMICAL resistance , *IXODIDAE , *MITES , *TICK control , *RHIPICEPHALUS , *IVERMECTIN - Abstract
The aim of this work was to analyse the spatiotemporal heterogeneity of Rhipicephalus microplus (Canestrini, 1888) (Acari: Ixodidae) resistance to chemical acaricides at intra‐farm level under different environmental (favourable and unfavourable areas for tick development) and management (different schemes of acaricides applications) conditions using ivermectin as a model. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in the different populations and subpopulations of R. microplus analysed. In the first case study, differences in resistance levels among tick samples within the same paddock across time and among tick samples from different paddocks were recorded. These results stress the importance of taking successive samples so that they represent the spatial and temporal variabilities in the levels of resistance that can occur within the same farm. In a second case study, the evolution of resistance in R. microplus subpopulations subjected to strategic and threshold control methods based on the application of three annual treatments with alternation of chemical groups was compared. No changes in resistance/susceptibility status were observed in both tick subpopulations. These results show that the application of a particular drug once a year within a scheme of alternation with other chemical groups could be an appropriate strategy to delay the development of resistance. Data of the third case study showed that environmental constraints is not only a key modulator of R. microplus abundance but could also affect the evolution of resistance in the tick populations. The decreasing trend of LC50 values in the tick subpopulation not exposed to chemical treatments but also in that subpopulation exposed to three annual chemical treatments under unfavourable environmental conditions allow us to hypothesise that environmental constraints can modify the levels of resistance in a tick population because it can constitute a stronger selection factor than the treatments themselves. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fluazuron in Argentina
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Torrents, Jorgelina, Sarli, Macarena, Sarmiento, Nestor F., Rossner, Maria V., Morel, Nicolas, Guglielmone, Alberto A., and Nava, Santiago
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- 2022
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8. Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina
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Torrents, Jorgelina, Sarli, Macarena, Rossner, Maria V., Toffaletti, José R., Morel, Nicolás, Martínez, Norberto C., Webster, Anelise, Mangold, Atilio J., Guglielmone, Alberto A., and Nava, Santiago
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- 2020
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9. In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina
- Author
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Torrents, Jorgelina, Morel, Nicolás, Rossner, Maria V., Martínez, Norberto C., Toffaletti, José R., and Nava, Santiago
- Published
- 2020
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10. A comparison of two in vitro bioassays to detect resistance of the cattle tick Rhipicephalus microplus to fipronil
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Torrents, Jorgelina, primary, Morel, Nicolas, additional, Rossner, Maria V., additional, Martínez, Norberto C., additional, and Nava, Santiago, additional
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- 2023
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11. Efecto del control estratégico de la garrapata común del bovino Rhipicephalus (Boophilus) microplus sobre la ganancia de peso en vaquillas Braford en el noreste de Argentina
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Rossner, Maria Victoria, Torrents, Jorgelina, Morel, Nicolas, Prieto, Paula Noelia, Lottero, F., Mangold, Atilio Jose, and Nava, Santiago
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Rhipicephalus (Boophilus) microplus ,Garrapatas ,Región Noroeste, Argentina ,Weight Gain ,Ticks ,Raza Braford ,Plagas de Animales ,Ganado Bovino ,Control de Plagas ,Rhipicephalus ,Metastigmata ,Ganancia de Peso ,Cattle ,Pest Control ,Pests of Animals - Abstract
El objetivo de este trabajo fue analizar el impacto del control estratégico de Rhipicephalus microplus sobre la ganancia de peso de vaquillas Braford en el noreste de Argentina. Se realizaron dos experimentos con un diseño similar en localidades de Corrientes (Colonia Tabay) y Chaco (Colonia Benítez). Los distintos grupos de vaquillas fueron sujetos a esquemas de control estratégico que consistieron en la aplicación de tres o cuatro productos garrapaticidas entre agosto y diciembre, y se compararon el número de garrapatas y la ganancia diaria de peso (GDP) entre estos y un grupo testigo que no recibió garrapaticidas. En Colonia Tabay, en los dos grupos tratados con esquemas de control estratégico la GDP fue significativamente mayor que en el grupo testigo con mayor nivel de infestación con garrapatas. El grupo sujeto a control estratégico en Colonia Benítez también tuvo una GDP significativamente mayor que el grupo testigo. Las diferencias en las GDP entre los grupos de animales sujetos a control estratégico y aquellos mantenidos como grupos testigo fluctuó entre 0.09 y 0.149 kg/día. Para el período evaluado en este trabajo (nueve meses), la aplicación del control estratégico contra R. microplus implicó una ganancia diferencial con respecto a los grupos sin tratamientos de 29 y 42 kg en promedio en las vaquillas de Colonia Tabay y de 25 kg en aquellas de Colonia Benítez. De los resultados se desprende que la aplicación de esquemas de tratamientos estratégicos para controlar la infestación con R. microplus en el noreste de Argentina contribuye a disminuir significativamente la abundancia de garrapatas y posibilita obtener mejores ganancias de peso vivo en bovinos en crecimiento al prevenir altos niveles de parasitación en los animales. The objective of this study was to analyze the impact of the strategic control of Rhipicephalus microplus on body weight gain of Braford heifers in northeastern Argentina. Two experiments with a similar design were carried out in Corrientes (Colonia Tabay) and Chaco (Colonia Benítez) Provinces. Strategic control schemes were applied different groups of heifers with the application of three or four treatments with chemical acaricides between August and December, and the number of ticks and the daily weight gain (GDP) were compared between them and with a control group without treatments. In Colonia Tabay, the GDP in the two groups treated with strategic control schemes was significantly higher than in the control group, which had higher level of infestation with ticks. The group subjected to strategic control in Colonia Benítez also had a significantly higher GDP than the control group. Differences in GDP between groups subjected to strategic control and control groups fluctuated between 0.09 and 0.149 kg/day. The application of strategic control against R. microplus implied a differential mean weight gain regarding the groups without treatments of 29 and 42 kg in Colonia Tabay heifers and 25 kg in Colonia Benítez. The results show that the application of strategic treatment schemes to control infestation with R. microplus in northeastern Argentina contributes to significantly reduce the abundance of ticks and enable obtain better body weight gains in growing cattle by preventing high levels of parasitism on animals. EEA Colonia Benitez EEA Rafaela Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Morel, Nicolas. Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET); Argentina Fil: Prieto, Paula Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benítez; Argentina Fil: Lottero, F. Fundación Correntina para la Sanidad Animal; Argentina Fil: Mangold, Atilio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Mangold, Atilio José. Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Nava, Santiago. Instituto de Investigación de la Cadena Láctea (IDICAL, INTA-CONICET); Argentina
- Published
- 2022
12. Influence of the cattle body weight estimation for drug dosing on the pharmacokinetic and efficacy of ivermectin against Rhipicephalus (Boophilus) microplus.
- Author
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Miró, María Victoria, Sarli, Macarena, Rossner, María Victoria, Gómez, Viviana, Toffaletti, José, Torrents, Jorgelina, Lifschitz, Adrián, and Nava, Santiago
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CATTLE weight ,RHIPICEPHALUS ,IVERMECTIN ,BODY weight ,PHARMACOKINETICS ,DRUG efficacy - Abstract
This study aimed to evaluate how the method of body weight estimation of a herd (average vs individual) for drug dosing may affect the pharmacokinetics and therapeutic efficacy of ivermectin 3.15% against Rhipicephalus (Boophilus) microplus. The trial was performed in two populations of R. (B.) microplus with different levels of susceptibility to ivermectin: San Martín (SM) (incipient resistance) and Colonia Benítez (CB) (susceptible). Twenty-one (SM) and forty-two (CB) 12-month-old Braford heifers, naturally infested with R. (B.) microplus, were individually weighed and divided into three groups (G). G1 and G2 were treated with a dose of ivermectin 3.15% (IVOMEC GOLD
® ) at a dose rate of 630 µg/kg of body weight (1 mL/50 kg). G1 received a uniform dose of ivermectin calculated according to the average of the individual body weights. G2 received a dose of ivermectin calculated according to the exact body weight of each animal. G3 were the control group. Ivermectin concentrations were measured from blood samples from day 1 to day 21 post-treatment. The efficacy of the treatment was evaluated by counting female ticks. In both populations the differences in the doses of ivermectin administered did not affect the pharmacokinetics and therapeutic efficacy of the drug. Nevertheless, it is necessary to avoid the sub-dosage of cattle to reduce the selection pressure for resistance on the tick populations. [ABSTRACT FROM AUTHOR]- Published
- 2022
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13. Eficacia reducida de derquantel y abamectina en ovinos y caprinos con Haemonchus sp resistentes a lactonas macrocíclicas
- Author
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Cerutti, Julieta, Cooper, Laura G., Torrents, Jorgelina, Suarez Archilla, Guillermo Alejandro, and Anziani, Oscar Sergio
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Caprinos ,Anthelmintics ,Sheep ,General Veterinary ,Abamectin ,Goats ,Biology ,Lactonas ,DERQUANTEL ,Ovinos ,Lactones ,Abamectina ,Antihelmínticos ,Derquantel ,Animal Science and Zoology ,Haemonchus ,Parasites ,Humanities ,Parásitos - Abstract
Se presentan las observaciones efectuadas sobre la actividad de una combinación antihelmíntica conteniendo un nueva droga, el derquantel (spiroindoles) adicionada con abamectina (lactonas macrocíclicas) en ovinos y caprinos naturalmente parasitados por Haemonchus sp. En el conteo de huevos, la eficacia de la combinación mostró porcentajes de reducción variables y dependientes de los estadios parasitarios intervinientes al momento del tratamiento y al status previo de resistencia a las lactonas macrocíclicas. Se observó una eficacia alta (mayor al 99%) frente a la oviposición de estadios maduros de Haemonchus sp o cuando los antecedentes de las poblaciones parasitarias indicaban resistencia no marcada a las lactonas macrocíclicas. Por el contrario, eficacias inferiores (76 al 83%) se observaron cuando las poblaciones de este parásito mostraban resistencia severa a las lactonas macrocíclicas y los estadios inmaduros constituían la mayor parte de la población parasitaria al momento del tratamiento. Las combinaciones antihelmínticas tienen un interesante potencial de uso frente a poblaciones parasitarias de Haemonchus sp resistentes a los antihelmínticos, pero las presentes observaciones enfatizan la necesidad de evaluar a nivel de cada establecimiento la actividad de todos los antiparasitarios, sean estos formulados con viejas o nuevas drogas, si se pretende instaurar programas racionales para el control de estos nematodes A field study was carried out to determine the activity of an anthelmintic combination of derquantel (spiroindoles) plus abamectin (macrocyclic lactones) against Haemonchus sp in sheeps and goats. The clinical efficacy of the combination showed variable egg count reduction percentage dependent on the parasitic stages involved at the time of treatment and the previous status of resistance to the macrocyclic lactones. High efficacy (greater than 99%) was observed against oviposition of mature Haemonchus sp stages or when background of parasitic populations indicated moderate resistance to macrocyclic lactones. In contrast, lower efficiencies (76-83%) were observed when populations of this parasite showed severe resistance to macrocyclic lactones and immature stages constituted the majority of the parasitic population at time of treatment. Anthelmintic combinations could have an interesting potential for prevent o manage the nematode resistance in small ruminants, but the present observations emphasize the need to evaluate at the level of each establishment the activity of all anthelmintic drenches formulated with new or old drugs EEA Rafaela Fil: Cerutti, Julieta. Universidad Católica de Córdoba. Facultad Ciencias Agropecuarias; Argentina Fil: Cooper, Laura G. Universidad Católica de Córdoba. Facultad Ciencias Agropecuarias; Argentina Fil: Torrents, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Suarez Archilla, Guillermo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Anziani, Oscar Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias, Argentina
- Published
- 2018
14. Pequeños estróngilos de los equinos. Eficacia clínica y periodo de reaparición de huevos luego del tratamiento con moxidectina y pirantel
- Author
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ANZIANI, Oscar S., COOPER, Laura G., CERUTTI, Julieta, FASSOLA, Luciana, TORRENTS, Jorgelina, MASNYJ, Franco, and CAFFE, Gabriel
- Abstract
El control de los pequeños estróngilos de los equinos (grupo Ciatostoma) se basa casi exclusivamente en la aplicación de antihelmínticos. En nuestro país, el desarrollo de resistencia generalizada a los bencimidazoles, está limitando las alternativas químicas disponibles a las lactonas macrocíclicas (ivermectina y moxidectina) y al pirantel, consideradas como drogas de larga y corta acción respectivamente. La información actualizada sobre la actividad de estas drogas en el campo es crítica para determinar su eficacia y detectar el desarrollo de fenómenos de resistencia a los antihelmínticos. En los equinos el período de reaparición de huevos (PRH) luego del tratamiento es considerado como un indicador temprano de la presencia de resistencia. En el presente trabajo se evaluó la eficacia clínica y el PRH luego de tratamientos con moxidectina y pirantel en equinos adultos de cinco establecimientos de las provincias de Santa Fe y Córdoba naturalmente parasitados por pequeños estróngilos. La eficacia clínica determinada al día 15 pos tratamiento utilizando un test de reducción en el conteo de huevos, osciló entre el 99,8 y el 100% para la moxidectina y entre el 98,9 y el 98,8% para el pirantel. Por su parte en el presente estudio el PRH fue de al menos 100 días para la moxidectina y de 35 días para el pirantel. Estos resultados indican que ambas drogas se muestran activas para el control de estos nematodes y que las poblaciones estudiadas (alguna de ellas resistentes a bencimidazoles) permanecen actualmente susceptibles a la moxidectina así como al pirantel. Esta última droga es de uso limitado en nuestro país, pero su inclusión en los programas de control contra los pequeños estróngilos podría reducir la dependencia y la presión de selección sobre las lactonas macrocíclicas y contribuir a mantener la vida útil de las mismas. The control of the small strongyles (Ciathostoma group) in horses is based on the application of anthelmintics. In our country, the development of generalized resistance to benzimidazoles is limiting the chemical alternatives available to macrocyclic lactones (ivermectin and moxidectin) and to pyrantel, considered as long-acting and short-acting drugs respectively. Updated information on the activity of these drugs in the field, is critical for determining its efficacy and detecting the development of anthelmintic resistance. In these horse nematodes the period of egg reappearance (ERP) after treatment is considered as an early indicator of the presence of resistance. The present study evaluated the clinical efficacy and ERP after moxidectin and pirantel treatments in adult horses naturally parasitized by small strongyles from five farms from Santa Fe and Córdoba provinces. Clinical efficacy determined at day 14 or 15 post treatment using a test of reduction in the egg count ranged from 98.9 to 98.8% for the pirantel and 99.8 to 100% for moxidectin. The ERP was at least 100 days for moxidectin and 35 days for the pirantel. These results indicate that both drugs are active for the control of these nematodes and that the populations studied (some of them resistant to benzimidazoles) remain currently susceptible to moxidectin as well as to pyrantel. This last drug is of limited use in Argentina, but its inclusion in the control programs against the small strongyls could reduce the dependence and the selection pressure on the macrocyclic lactones and contribute to maintain the useful life of the same ones.
- Published
- 2017
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15. Eficacia del monepantel para el control de aislamientos de Haemonchus contortus y Trichostrongylus spp con resistencia múltiple (ivermectina y febendazole) en caprinos
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COOPER, Laura, CERUTTI, Julieta, MOHN, Cristian, TORRENTS, Jorgelina, SUAREZ ARCHILLA, Guillermo, and ANZIANI, Oscar
- Abstract
El monepantel es un nuevo antihelmíntico registrado en nuestro país exclusivamente para el control de los nematodes gastrointestinales de los ovinos y su uso ha sido direccionado mayormente hacia el control de parásitos resistentes a las clases de antihelmínticos disponibles actualmente. Estos mismos nematodes también parasitan a los caprinos, pero en estos rumiantes la pato-fisiología y la respuesta a los antihelmínticos es diferente, lo cual resulta en un mayor parasitismo y complejidad en el manejo de la resistencia. La presente comunicación informa sobre la eficacia del monepantel en dos hatos caprinos mantenidos bajo condiciones de campo y parasitados naturalmente por los géneros de nematodes gastrointestinales más comunes del área central de la Argentina (Haemonchus y Trichostrongylus) y portando alelos de resistencia múltiple (ivermectina y febendazole). El test de reducción en el conteo de huevos post tratamiento comparando diversas fórmulas, indicaron que en todos los hatos el monepantel por vía oral y a la dosis de 3,75 mg/kg de peso (1,5 veces mayor a la dosis ovina), resultó en eficacias del 99% al 100 %. Se realizan breves consideraciones sobre el uso potencial de esta droga en caprinos. Monepantel is a new anthelmintic registered in Argentina exclusively for control of gastrointestinal nematodes of sheep and mostly directed toward controlling resistant parasites to current available classes of anthelmintics. The same nematodes also parasitize goats but pathophysiology and response are different in these hosts resulting in more severe parasitism and complexity in handling anthelmintic resistance. This report assess the efficacy of monepantel in goats maintained under field conditions and naturally parasitized by most common gastrointestinal nematodes from central Argentina (Haemonchus and Trichostrongylus) carrying alleles of multiple resistance (ivermectin and febendazole). According to the egg count reduction test, efficacies of 99% to 100% were observed after monepantel treatment at 3.75 mg/kg orally (1.5 ovine dose).
- Published
- 2016
- Full Text
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16. Monepatel y control de Haemonchus contortus en ovinos. Evaluación de la eficacia luego de cuatro años de uso en condiciones de refugio reducido
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TORRENTS, Jorgelina, SUAREZ ARCHILLA, Guillermo A., and ANZIANI, Oscar S
- Abstract
Resistance to anthelmintics in sheep nematode is widespread in central-northern Argentina and current major concern is the rise and spread of Haemonchus contortus carrying multiple resistance alleles. Currently, monepantel and derquantel are the only broad-spectrum anthelmintics without documentation of resistance in our country. With the first of these drugs, have already been registered cases of resistance in Uruguay and Brazil so it is critical update on the activity in the field of these anthelmintics. This study aims to test the effectiveness of monepantel after four years of use of this drug on an isolation of H. contortus with multiple resistance (macrocyclic lactones, closantel, levamisole and benzimidazole) and under conditions of reduced refugia. Two experiences, A and B (2014 and 2016 respectively), were carried out with lambs naturally parasitized and selected by the number of eggs per gram of feces (≥ 250). In both experiences, the test of reduction in egg counts after treatment with monepantel (2.5 mgr/Kg orally) comparing various formulas indicated that the drug remained with efficacy greater than 97% after four years of use to control this isolation of H. contortus under the field conditions of these studies. La resistencia de los nematodes ovinos a los antihelmínticos está generalizada en el centro-norte de la Argentina y la mayor preocupación actual es el aumento y dispersión de Haemonchus contortus portando alelos con resistencia múltiple. Actualmente los únicos antihelmínticos de amplio espectro sin documentación de resistencia en nuestro país son el monepantel y derquantel. Con la primera de estas drogas ya se han registrado casos de resistencia en Uruguay y Brasil por lo que es crítica la información actualizada sobre la actividad en el campo de estos antihelmínticos. Este estudio informa sobre la eficacia del monepantel luego de cuatro años de uso de esta droga sobre un aislamiento de H. contortus con resistencia múltiple (lactonas macrociclicas, closantel, levamisol y benzimidazoles) y bajo condiciones de refugio reducido. Se realizaron dos experiencias A y B (años 2014 y 2016 respectivamente) con corderos naturalmente parasitados y seleccionados en función del número de huevos por gramo de heces (≥ 250) los que fueron tratados con monepantel (2,5 mg/Kg oral). El test de reducción en el conteo de huevos post tratamiento comparando diversas fórmulas indicó que en ambas experiencias, la droga mantuvo una eficacia mayor al 97 % y que permanece activa para controlar este aislamiento de H. contortus luego de cuatro años de uso y bajo las particulares condiciones en las que se realizaron estos estudios. 
- Published
- 2016
- Full Text
- View/download PDF
17. Two novel Ehrlichia(Rickettsiales: Anaplasmataceae) strains detected in ticks (Ixodida, Ixodidae) and opossums (Didelphimorphia: Didelphidae) in Argentina
- Author
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Tarragona, Evelina L., Flores, Fernando S., Lamattina, Daniela, Torrents, Jorgelina, Sebastian, Patrick S., and Nava, Santiago
- Abstract
The aim of this study is to determine if there is circulation of microorganisms of the genus Ehrlichiain opossums Didelphis albiventrisand their ticks from the Humid Chaco in Argentina. Blood samples of 15 specimens of the opossum D. albiventriswere analysed. Immature stages of the ticks Amblyomma ovale(Larvae=26; Nymphs=10), Amblyomma sculptum(Larvae=86; Nymphs=6) and Ornithodorossp. cf. O. mimon(Larvae=90) were also analyzed. DNA was extracted individually from blood samples and ticks. Molecular detection of Ehrlichiaagents was performed targeting two different loci: 16S rRNA and dsb gen. The phylogenetic analyses showed that the Ehrlichiasp. detected in D. albiventrisin this study is identical to Ehrlichiasp. strain Natal previously detected in two marsupials from Brazil. Furthermore, a new Ehrlichia strain was amplified from an A. ovalenymph (named as Ehrlichiasp. strain El Bagual) which is phylogenetically closely related to a strain of Ehrlichiasp. detected in Bradypus tridactylusin Brazil. The findings of the current study represent the first report of these two strains of Ehrlichiafor Argentina, showing that the diversity of Ehrlichiaspp. is greater than previously assumed. Further studies should determine the epidemiological relevance of these findings.
- Published
- 2022
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18. Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplusticks from Argentina
- Author
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Sarli, Macarena, Torrents, Jorgelina, Morel, Nicolás, and Nava, Santiago
- Abstract
This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplusticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from five localities in northern Argentina. Tick populations were tested for fipronil resistance by in vitro larval packet test. DNA encoding amino acid residues 264–360 of the GABA-Cl was amplified by PCR. PCR products were sequenced and the translated amino acid sequences were aligned and compared among each other and with sequences of the GABA-Cl belonging to the Brazilian and Uruguayan reference strains fipronil-susceptible (Mozo) and resistant (RFSan and Juarez). All the GABA-Cl amino acid sequences obtained from the R. microplusspecimens from Argentina were identical, regardless of the fipronil resistance-susceptibility status of each population. Mutations present in GABA-Cl sequence of RFSan and Juarez strains were not present in any of the populations from Argentina analyzed. These results indicate the failure of this gene as a molecular marker of resistance to fipronil, at least in the R. micropluspopulations from northern Argentina.
- Published
- 2022
- Full Text
- View/download PDF
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