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1. A global experiment on motivating social distancing during the COVID-19 pandemic

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Legate, N, Nguyen, T, Weinstein, N, Moller, A, Legault, L, Vally, Z, Tajchman, Z, Zsido, A, Zrimsek, M, Chen, Z, Ziano, I, Gialitaki, Z, Ceary, C, Jang, Y, Lin, Y, Kunisato, Y, Yamada, Y, Xiao, Q, Jiang, X, Du, X, Yao, E, Ryan, W, Wilson, J, Cyrus-Lai, W, Jimenez-Leal, W, Law, W, Unanue, W, Collins, W, Richard, K, Vranka, M, Ankushev, V, Schei, V, Lerche, V, Kovic, V, Krizanic, V, Kadreva, V, Adoric, V, Tran, U, Yeung, S, Hassan, W, Houston, R, Machin, M, Lima, T, Ostermann, T, Frizzo, T, Sverdrup, T, House, T, Gill, T, Fedotov, M, Paltrow, T, Jernsather, T, Rahman, T, Machin, T, Koptjevskaja-Tamm, M, Hostler, T, Ishii, T, Szaszi, B, Adamus, S, Suter, L, von Bormann, S, Habib, S, Studzinska, A, Stojanovska, D, Janssen, S, Stieger, S, Schulenberg, S, Tatachari, S, Azouaghe, S, Sorokowski, P, Sorokowska, A, Song, X, Morbee, S, Lewis, S, Sinkolova, S, Grigoryev, D, Drexler, S, Daches, S, Levine, S, Geniole, S, Akter, S, Vracar, S, Massoni, S, Costa, S, Zorjan, S, Sarioguz, E, Izquierdo, S, Tshonda, S, Alves, S, Pontinen, S, Solas, S, Ordonez-Riano, S, Ocovaj, S, Onie, S, Lins, S, Biberauer, T, Coksan, S, Khumkom, S, Sacakli, A, Ruiz-Fernandez, S, Geiger, S, Modares, S, Walczak, R, Betlehem, R, Vilar, R, Carcamo, R, Ross, R, Mccarthy, R, Ballantyne, T, Westgate, E, Ryan, R, Gargurevich, R, Afhami, R, Ren, D, Monteiro, R, Reips, U, Reggev, N, Calin-Jagema, R, Pourafshari, R, Oliveira, R, Nedelcheva-Datsova, M, Rahal, R, Ribeiro, R, Radtke, T, Searston, R, Jai-ai, R, Habte, R, Zdybek, P, Chen, S, Wajanatinapart, P, Maturan, P, Perillo, J, Isager, P, Kacmar, P, Macapagal, P, Maniaci, M, Szwed, P, Hanel, P, Forbes, P, Arriaga, P, Paris, B, Parashar, N, Papachristopoulos, K, Correa, P, Kacha, O, Bernardo, M, Campos, O, Bravo, O, Galindo-Caballero, O, Ogbonnaya, C, Bialobrzeska, O, Kiselnikova, N, Simonovic, N, Cohen, N, Nock, N, Hernandez, A, Thogersen-Ntouma, C, Ntoumanis, N, Johannes, N, Albayrak-Aydemir, N, Say, N, Neubauer, A, Martin, N, Torunsky, N, van Antwerpen, N, Van Doren, N, Sunami, N, Rachev, N, Majeed, N, Schmidt, N, Nadif, K, Corral-Frias, N, Ouherrou, N, Abbas, N, Pantazi, M, Lucas, M, Vasilev, M, Ortiz, M, Butt, M, Kurfali, M, Kabir, M, Muda, R, Rivera, M, Sirota, M, Seehuus, M, Parzuchowski, M, Toro, M, Hricova, M, Maldonado, M, Rentzelas, P, Vansteenkiste, M, Metz, M, Marszalek, M, Karekla, M, Mioni, G, Bosma, M, Westerlund, M, Vdovic, M, Bialek, M, Silan, M, Anne, M, Misiak, M, Gugliandolo, M, Grinberg, M, Capizzi, M, Barria, M, Mensink, M, Harutyunyan, M, Khosla, M, Dunn, M, Korbmacher, M, Adamkovic, M, Ribeiro, M, Terskova, M, Hruska, M, Martoncik, M, Voracek, M, Cadek, M, Frias-Armenta, M, Kowal, M, Topor, M, Roczniewska, M, Oosterlinck, M, Kohlova, M, Paruzel-Czachura, M, Sabristov, M, Romanova, M, Papadatou-Pastou, M, Lund, M, Antoniadi, M, Magrin, M, Jones, M, Li, M, Manavalan, M, Muminov, A, Kossowska, M, Friedemann, M, Wielgus, M, van Hooff, M, Varella, M, Standage, M, Nicolotti, M, Colloff, M, Bradford, M, Vaughn, L, Eudave, L, Vieira, L, Lu, J, Pineda, L, Matos, L, Perez, L, Lazarevic, L, Jaremka, L, Smit, E, Kushnir, E, Ferguson, L, Anton-Boicuk, L, Coelho, G, Ahlgren, L, Liga, F, Levitan, C, Micheli, L, Gunton, L, Volz, L, Stojanovska, M, Boucher, L, Samojlenko, L, Delgado, L, Kaliska, L, Beatrix, L, Warmelink, L, Rojas-Berscia, L, Yu, K, Wylie, K, Wachowicz, J, Desai, K, Barzykowski, K, Kozma, L, Evans, K, Kirgizova, K, Agesin, B, Koehn, M, Wolfe, K, Korobova, T, Morris, K, Klevjer, K, van Schie, K, Vezirian, K, Damnjanovic, K, Thommesen, K, Schmidt, K, Filip, K, Staniaszek, K, Grzech, K, Hoyer, K, Moon, K, Khaobunmasiri, S, Rana, K, Janjic, K, Suchow, J, Kielinska, J, Vasquez, J, Chanal, J, Beitner, J, Vargas-Nieto, J, Roxas, J, Taber, J, Urriago-Rayo, J, Pavlacic, J, Benka, J, Bavolar, J, Soto, J, Olofsson, J, Vilsmeier, J, Messerschmidt, J, Czamanski-Cohen, J, Waterschoot, J, Moss, J, Boudesseul, J, Lee, J, Kamburidis, J, Joy-Gaba, J, Zickfeld, J, Miranda, J, Verharen, J, Hristova, E, Beshears, J, Djordjevic, J, Bosch, J, Valentova, J, Antfolk, J, Berkessel, J, Schrotter, J, Urban, J, Roer, J, Norton, J, Silva, J, Pickering, J, Vintr, J, Uttley, J, Kunst, J, Ndukaihe, I, Iyer, A, Vilares, I, Ivanov, A, Ropovik, I, Sula, I, Sarieva, I, Metin-Orta, I, Prusova, I, Pinto, I, Bozdoc, A, Almeida, I, Pit, I, Dalgar, I, Zakharov, I, Arinze, A, Ihaya, K, Stephen, I, Gjoneska, B, Brohmer, H, Flowe, H, Godbersen, H, Kocalar, H, Hedgebeth, M, Chuan-Peng, H, Sharifian, M, Manley, H, Akkas, H, Hajdu, N, Azab, H, Kaminski, G, Nilsonne, G, Anjum, G, Travaglino, G, Feldman, G, Pfuhl, G, Czarnek, G, Marcu, G, Hofer, G, Banik, G, Adetula, G, Bijlstra, G, Verbruggen, F, Kung, F, Martela, F, Foroni, F, Forest, J, Singer, G, Muchembled, F, Azevedo, F, Mosannenzadeh, F, Marinova, E, Strukelj, E, Etebari, Z, Bradshaw, E, Baskin, E, Garcia, E, Musser, E, van Steenkiste, I, Ahn, E, Quested, E, Pronizius, E, Jackson, E, Manunta, E, Agadullina, E, Sakan, D, Dursun, P, Dujols, O, Dubrov, D, Willis, M, Tumer, M, Beaudry, J, Popovic, D, Dunleavy, D, Djamai, I, Krupic, D, Herrera, D, Vega, D, Du, H, Mola, D, Chakarova, D, Davis, W, Holford, D, Lewis, D, Vaidis, D, Ozery, D, Ricaurte, D, Storage, D, Sousa, D, Alvarez, D, Boller, D, Dalla Rosa, A, Dimova, D, Marko, D, Moreau, D, Reeck, C, Correia, R, Whitt, C, Lamm, C, Solorzano, C, von Bastian, C, Sutherland, C, Overkott, C, Aberson, C, Wang, C, Niemiec, C, Karashiali, C, Noone, C, Chiu, F, Picciocchi, C, Brownlow, C, Karaarslan, C, Cellini, N, Esteban-Serna, C, Reyna, C, Ferreyra, C, Batres, C, Li, R, Grano, C, Carpentier, J, Tamnes, C, Fu, C, Ishkhanyan, B, Bylinina, L, Jaeger, B, Bundt, C, Allred, T, Vermote, B, Bokkour, A, Bogatyreva, N, Shi, J, Chopik, W, Antazo, B, Behzadnia, B, Becker, M, Bayyat, M, Cocco, B, Chou, W, Barkoukis, V, Hubena, B, Zuro, B, Aczel, B, Baklanova, E, Bai, H, Balci, B, Babincak, P, Soenens, B, Dixson, B, Mokady, A, Kappes, H, Atari, M, Szala, A, Szabelska, A, Aruta, J, Domurat, A, Arinze, N, Modena, A, Adiguzel, A, Monajem, A, El Arabi, K, Ozdogru, A, Rothbaum, A, Torres, A, Theodoropoulou, A, Skowronek, A, Jurkovic, A, Singh, A, Kassianos, A, Findor, A, Hartanto, A, Landry, A, Ferreira, A, Santos, A, De la Rosa-Gomez, A, Gourdon-Kanhukamwe, A, Luxon, A, Todsen, A, Karababa, A, Janak, A, Pilato, A, Bran, A, Tullett, A, Kuzminska, A, Krafnick, A, Urooj, A, Khaoudi, A, Ahmed, A, Groyecka-Bernard, A, Askelund, A, Adetula, A, Belaus, A, Charyate, A, Wichman, A, Stoyanova, A, Greenburgh, A, Thomas, A, Arvanitis, A, Forscher, P, Mallik, P, Coles, N, Miller, J, Moshontz, H, Urry, H, Ijzerman, H, Basnight-Brown, D, Ebersole, C, Chartier, C, Buchanan, E, Primbs, M, Nguyen, TV, Zsido, AN, Ceary, CD, Jang, YN, Lin, YJ, Xiao, QY, Jiang, XM, Du, XK, Ryan, WS, Wilson, JP, Collins, WM, Richard, KL, Kadreva, VH, Adoric, VC, Tran, US, Yeung, SK, Machin, MA, Lima, TJS, Sverdrup, TE, Hostler, TJ, von Bormann, SM, Janssen, SMJ, Schulenberg, SE, Drexler, SM, Levine, SL, Geniole, SN, Izquierdo, SM, Tshonda, SS, Alves, SG, Solas, SA, Ocovaj, SB, Geiger, SJ, Modares, SF, Walczak, RB, Carcamo, RA, Ross, RM, McCarthy, R, Westgate, EC, Ryan, RM, Ren, DN, Monteiro, RP, Reips, UD, Calin-Jagema, RJ, Rahal, RM, Ribeiro, RR, Chen, SC, Maturan, PLG, Perillo, JT, Isager, PM, Macapagal, PM, Maniaci, MR, Hanel, PHP, Forbes, PAG, Correa, PS, Bravo, ON, Galindo-Caballero, OJ, Ogbonnaya, CE, Nock, NL, Neubauer, AB, Martin, NI, Rachev, NR, Majeed, NM, Schmidt, ND, Corral-Frias, NS, Lucas, MY, Vasilev, MR, Ortiz, MV, Butt, MM, Rivera, MDT, Maldonado, MA, Metz, MA, Bosma, MJ, Silan, MA, Gugliandolo, MC, Barria, MFE, Kurfali, MA, Mensink, MC, Dunn, MR, Ribeiro, MFF, Kohlova, MB, Lund, ML, Magrin, ME, Jones, MV, Li, MY, Ortiz, MS, van Hooff, MLM, Varella, MAC, Colloff, MF, Vaughn, LA, Lu, JG, Pineda, LMS, Perez, LC, Lazarevic, LB, Jaremka, LM, Smit, ES, Ferguson, LJ, Coelho, GLD, Levitan, CA, Gunton, LA, Delgado, LGJ, Rojas-Berscia, LM, Agesin, BBE, Koehn, MA, Thommesen, KK, Suchow, JW, Vasquez, JEC, Vargas-Nieto, JC, Roxas, JCT, Pavlacic, JM, Soto, JA, Olofsson, JK, Vilsmeier, JK, Moss, JD, Lee, JM, Joy-Gaba, JA, Miranda, JF, Verharen, JPH, Beshears, JE, Djordjevic, JM, Valentova, JV, Berkessel, JB, Roer, JP, Norton, JO, Silva, JR, Pickering, JS, Kunst, JR, Ndukaihe, ILG, Bozdoc, AI, Almeida, IAT, Pit, IL, Arinze, AI, Stephen, ID, Kocalar, HE, Hedgebeth, MV, Travaglino, GA, Marcu, GM, Adetula, GA, Kung, FYH, Bradshaw, EL, Garcia, EOL, van Steenkiste, IMM, Ahn, ER, Jackson, EA, Beaudry, JL, Du, HF, Davis, WE, Holford, DL, Lewis, DMG, Vaidis, DC, Ozery, DH, Ricaurte, DZ, Alvarez, DS, Correia, RC, Whitt, CM, Solorzano, CS, von Bastian, CC, Sutherland, CAM, Aberson, CL, Wang, CH, Niemiec, CP, Li, RR, Tamnes, CK, Fu, CHY, Allred, TB, Vermote, BJ, Shi, JX, Chopik, WJ, Bayyat, MM, Chou, WL, Balci, BB, Dixson, BJW, Kappes, HB, Aruta, JJB, Arinze, NC, El Arabi, KA, Ozdogru, AA, Rothbaum, AO, Torres, AO, Jurkovic, AP, Kassianos, AP, Landry, AT, Santos, AC, Luxon, AM, Todsen, AL, Tullett, AM, Kuzminska, AO, Krafnick, AJ, Askelund, AD, Charyate, AC, Wichman, AL, Thomas, AG, Forscher, PS, Mallik, PR, Coles, NA, Miller, JK, Urry, HL, IJzerman, H, Basnight-Brown, DM, Ebersole, CR, Chartier, CR, Buchanan, EM, Primbs, MA, Legate, N, Nguyen, T, Weinstein, N, Moller, A, Legault, L, Vally, Z, Tajchman, Z, Zsido, A, Zrimsek, M, Chen, Z, Ziano, I, Gialitaki, Z, Ceary, C, Jang, Y, Lin, Y, Kunisato, Y, Yamada, Y, Xiao, Q, Jiang, X, Du, X, Yao, E, Ryan, W, Wilson, J, Cyrus-Lai, W, Jimenez-Leal, W, Law, W, Unanue, W, Collins, W, Richard, K, Vranka, M, Ankushev, V, Schei, V, Lerche, V, Kovic, V, Krizanic, V, Kadreva, V, Adoric, V, Tran, U, Yeung, S, Hassan, W, Houston, R, Machin, M, Lima, T, Ostermann, T, Frizzo, T, Sverdrup, T, House, T, Gill, T, Fedotov, M, Paltrow, T, Jernsather, T, Rahman, T, Machin, T, Koptjevskaja-Tamm, M, Hostler, T, Ishii, T, Szaszi, B, Adamus, S, Suter, L, von Bormann, S, Habib, S, Studzinska, A, Stojanovska, D, Janssen, S, Stieger, S, Schulenberg, S, Tatachari, S, Azouaghe, S, Sorokowski, P, Sorokowska, A, Song, X, Morbee, S, Lewis, S, Sinkolova, S, Grigoryev, D, Drexler, S, Daches, S, Levine, S, Geniole, S, Akter, S, Vracar, S, Massoni, S, Costa, S, Zorjan, S, Sarioguz, E, Izquierdo, S, Tshonda, S, Alves, S, Pontinen, S, Solas, S, Ordonez-Riano, S, Ocovaj, S, Onie, S, Lins, S, Biberauer, T, Coksan, S, Khumkom, S, Sacakli, A, Ruiz-Fernandez, S, Geiger, S, Modares, S, Walczak, R, Betlehem, R, Vilar, R, Carcamo, R, Ross, R, Mccarthy, R, Ballantyne, T, Westgate, E, Ryan, R, Gargurevich, R, Afhami, R, Ren, D, Monteiro, R, Reips, U, Reggev, N, Calin-Jagema, R, Pourafshari, R, Oliveira, R, Nedelcheva-Datsova, M, Rahal, R, Ribeiro, R, Radtke, T, Searston, R, Jai-ai, R, Habte, R, Zdybek, P, Chen, S, Wajanatinapart, P, Maturan, P, Perillo, J, Isager, P, Kacmar, P, Macapagal, P, Maniaci, M, Szwed, P, Hanel, P, Forbes, P, Arriaga, P, Paris, B, Parashar, N, Papachristopoulos, K, Correa, P, Kacha, O, Bernardo, M, Campos, O, Bravo, O, Galindo-Caballero, O, Ogbonnaya, C, Bialobrzeska, O, Kiselnikova, N, Simonovic, N, Cohen, N, Nock, N, Hernandez, A, Thogersen-Ntouma, C, Ntoumanis, N, Johannes, N, Albayrak-Aydemir, N, Say, N, Neubauer, A, Martin, N, Torunsky, N, van Antwerpen, N, Van Doren, N, Sunami, N, Rachev, N, Majeed, N, Schmidt, N, Nadif, K, Corral-Frias, N, Ouherrou, N, Abbas, N, Pantazi, M, Lucas, M, Vasilev, M, Ortiz, M, Butt, M, Kurfali, M, Kabir, M, Muda, R, Rivera, M, Sirota, M, Seehuus, M, Parzuchowski, M, Toro, M, Hricova, M, Maldonado, M, Rentzelas, P, Vansteenkiste, M, Metz, M, Marszalek, M, Karekla, M, Mioni, G, Bosma, M, Westerlund, M, Vdovic, M, Bialek, M, Silan, M, Anne, M, Misiak, M, Gugliandolo, M, Grinberg, M, Capizzi, M, Barria, M, Mensink, M, Harutyunyan, M, Khosla, M, Dunn, M, Korbmacher, M, Adamkovic, M, Ribeiro, M, Terskova, M, Hruska, M, Martoncik, M, Voracek, M, Cadek, M, Frias-Armenta, M, Kowal, M, Topor, M, Roczniewska, M, Oosterlinck, M, Kohlova, M, Paruzel-Czachura, M, Sabristov, M, Romanova, M, Papadatou-Pastou, M, Lund, M, Antoniadi, M, Magrin, M, Jones, M, Li, M, Manavalan, M, Muminov, A, Kossowska, M, Friedemann, M, Wielgus, M, van Hooff, M, Varella, M, Standage, M, Nicolotti, M, Colloff, M, Bradford, M, Vaughn, L, Eudave, L, Vieira, L, Lu, J, Pineda, L, Matos, L, Perez, L, Lazarevic, L, Jaremka, L, Smit, E, Kushnir, E, Ferguson, L, Anton-Boicuk, L, Coelho, G, Ahlgren, L, Liga, F, Levitan, C, Micheli, L, Gunton, L, Volz, L, Stojanovska, M, Boucher, L, Samojlenko, L, Delgado, L, Kaliska, L, Beatrix, L, Warmelink, L, Rojas-Berscia, L, Yu, K, Wylie, K, Wachowicz, J, Desai, K, Barzykowski, K, Kozma, L, Evans, K, Kirgizova, K, Agesin, B, Koehn, M, Wolfe, K, Korobova, T, Morris, K, Klevjer, K, van Schie, K, Vezirian, K, Damnjanovic, K, Thommesen, K, Schmidt, K, Filip, K, Staniaszek, K, Grzech, K, Hoyer, K, Moon, K, Khaobunmasiri, S, Rana, K, Janjic, K, Suchow, J, Kielinska, J, Vasquez, J, Chanal, J, Beitner, J, Vargas-Nieto, J, Roxas, J, Taber, J, Urriago-Rayo, J, Pavlacic, J, Benka, J, Bavolar, J, Soto, J, Olofsson, J, Vilsmeier, J, Messerschmidt, J, Czamanski-Cohen, J, Waterschoot, J, Moss, J, Boudesseul, J, Lee, J, Kamburidis, J, Joy-Gaba, J, Zickfeld, J, Miranda, J, Verharen, J, Hristova, E, Beshears, J, Djordjevic, J, Bosch, J, Valentova, J, Antfolk, J, Berkessel, J, Schrotter, J, Urban, J, Roer, J, Norton, J, Silva, J, Pickering, J, Vintr, J, Uttley, J, Kunst, J, Ndukaihe, I, Iyer, A, Vilares, I, Ivanov, A, Ropovik, I, Sula, I, Sarieva, I, Metin-Orta, I, Prusova, I, Pinto, I, Bozdoc, A, Almeida, I, Pit, I, Dalgar, I, Zakharov, I, Arinze, A, Ihaya, K, Stephen, I, Gjoneska, B, Brohmer, H, Flowe, H, Godbersen, H, Kocalar, H, Hedgebeth, M, Chuan-Peng, H, Sharifian, M, Manley, H, Akkas, H, Hajdu, N, Azab, H, Kaminski, G, Nilsonne, G, Anjum, G, Travaglino, G, Feldman, G, Pfuhl, G, Czarnek, G, Marcu, G, Hofer, G, Banik, G, Adetula, G, Bijlstra, G, Verbruggen, F, Kung, F, Martela, F, Foroni, F, Forest, J, Singer, G, Muchembled, F, Azevedo, F, Mosannenzadeh, F, Marinova, E, Strukelj, E, Etebari, Z, Bradshaw, E, Baskin, E, Garcia, E, Musser, E, van Steenkiste, I, Ahn, E, Quested, E, Pronizius, E, Jackson, E, Manunta, E, Agadullina, E, Sakan, D, Dursun, P, Dujols, O, Dubrov, D, Willis, M, Tumer, M, Beaudry, J, Popovic, D, Dunleavy, D, Djamai, I, Krupic, D, Herrera, D, Vega, D, Du, H, Mola, D, Chakarova, D, Davis, W, Holford, D, Lewis, D, Vaidis, D, Ozery, D, Ricaurte, D, Storage, D, Sousa, D, Alvarez, D, Boller, D, Dalla Rosa, A, Dimova, D, Marko, D, Moreau, D, Reeck, C, Correia, R, Whitt, C, Lamm, C, Solorzano, C, von Bastian, C, Sutherland, C, Overkott, C, Aberson, C, Wang, C, Niemiec, C, Karashiali, C, Noone, C, Chiu, F, Picciocchi, C, Brownlow, C, Karaarslan, C, Cellini, N, Esteban-Serna, C, Reyna, C, Ferreyra, C, Batres, C, Li, R, Grano, C, Carpentier, J, Tamnes, C, Fu, C, Ishkhanyan, B, Bylinina, L, Jaeger, B, Bundt, C, Allred, T, Vermote, B, Bokkour, A, Bogatyreva, N, Shi, J, Chopik, W, Antazo, B, Behzadnia, B, Becker, M, Bayyat, M, Cocco, B, Chou, W, Barkoukis, V, Hubena, B, Zuro, B, Aczel, B, Baklanova, E, Bai, H, Balci, B, Babincak, P, Soenens, B, Dixson, B, Mokady, A, Kappes, H, Atari, M, Szala, A, Szabelska, A, Aruta, J, Domurat, A, Arinze, N, Modena, A, Adiguzel, A, Monajem, A, El Arabi, K, Ozdogru, A, Rothbaum, A, Torres, A, Theodoropoulou, A, Skowronek, A, Jurkovic, A, Singh, A, Kassianos, A, Findor, A, Hartanto, A, Landry, A, Ferreira, A, Santos, A, De la Rosa-Gomez, A, Gourdon-Kanhukamwe, A, Luxon, A, Todsen, A, Karababa, A, Janak, A, Pilato, A, Bran, A, Tullett, A, Kuzminska, A, Krafnick, A, Urooj, A, Khaoudi, A, Ahmed, A, Groyecka-Bernard, A, Askelund, A, Adetula, A, Belaus, A, Charyate, A, Wichman, A, Stoyanova, A, Greenburgh, A, Thomas, A, Arvanitis, A, Forscher, P, Mallik, P, Coles, N, Miller, J, Moshontz, H, Urry, H, Ijzerman, H, Basnight-Brown, D, Ebersole, C, Chartier, C, Buchanan, E, Primbs, M, Nguyen, TV, Zsido, AN, Ceary, CD, Jang, YN, Lin, YJ, Xiao, QY, Jiang, XM, Du, XK, Ryan, WS, Wilson, JP, Collins, WM, Richard, KL, Kadreva, VH, Adoric, VC, Tran, US, Yeung, SK, Machin, MA, Lima, TJS, Sverdrup, TE, Hostler, TJ, von Bormann, SM, Janssen, SMJ, Schulenberg, SE, Drexler, SM, Levine, SL, Geniole, SN, Izquierdo, SM, Tshonda, SS, Alves, SG, Solas, SA, Ocovaj, SB, Geiger, SJ, Modares, SF, Walczak, RB, Carcamo, RA, Ross, RM, McCarthy, R, Westgate, EC, Ryan, RM, Ren, DN, Monteiro, RP, Reips, UD, Calin-Jagema, RJ, Rahal, RM, Ribeiro, RR, Chen, SC, Maturan, PLG, Perillo, JT, Isager, PM, Macapagal, PM, Maniaci, MR, Hanel, PHP, Forbes, PAG, Correa, PS, Bravo, ON, Galindo-Caballero, OJ, Ogbonnaya, CE, Nock, NL, Neubauer, AB, Martin, NI, Rachev, NR, Majeed, NM, Schmidt, ND, Corral-Frias, NS, Lucas, MY, Vasilev, MR, Ortiz, MV, Butt, MM, Rivera, MDT, Maldonado, MA, Metz, MA, Bosma, MJ, Silan, MA, Gugliandolo, MC, Barria, MFE, Kurfali, MA, Mensink, MC, Dunn, MR, Ribeiro, MFF, Kohlova, MB, Lund, ML, Magrin, ME, Jones, MV, Li, MY, Ortiz, MS, van Hooff, MLM, Varella, MAC, Colloff, MF, Vaughn, LA, Lu, JG, Pineda, LMS, Perez, LC, Lazarevic, LB, Jaremka, LM, Smit, ES, Ferguson, LJ, Coelho, GLD, Levitan, CA, Gunton, LA, Delgado, LGJ, Rojas-Berscia, LM, Agesin, BBE, Koehn, MA, Thommesen, KK, Suchow, JW, Vasquez, JEC, Vargas-Nieto, JC, Roxas, JCT, Pavlacic, JM, Soto, JA, Olofsson, JK, Vilsmeier, JK, Moss, JD, Lee, JM, Joy-Gaba, JA, Miranda, JF, Verharen, JPH, Beshears, JE, Djordjevic, JM, Valentova, JV, Berkessel, JB, Roer, JP, Norton, JO, Silva, JR, Pickering, JS, Kunst, JR, Ndukaihe, ILG, Bozdoc, AI, Almeida, IAT, Pit, IL, Arinze, AI, Stephen, ID, Kocalar, HE, Hedgebeth, MV, Travaglino, GA, Marcu, GM, Adetula, GA, Kung, FYH, Bradshaw, EL, Garcia, EOL, van Steenkiste, IMM, Ahn, ER, Jackson, EA, Beaudry, JL, Du, HF, Davis, WE, Holford, DL, Lewis, DMG, Vaidis, DC, Ozery, DH, Ricaurte, DZ, Alvarez, DS, Correia, RC, Whitt, CM, Solorzano, CS, von Bastian, CC, Sutherland, CAM, Aberson, CL, Wang, CH, Niemiec, CP, Li, RR, Tamnes, CK, Fu, CHY, Allred, TB, Vermote, BJ, Shi, JX, Chopik, WJ, Bayyat, MM, Chou, WL, Balci, BB, Dixson, BJW, Kappes, HB, Aruta, JJB, Arinze, NC, El Arabi, KA, Ozdogru, AA, Rothbaum, AO, Torres, AO, Jurkovic, AP, Kassianos, AP, Landry, AT, Santos, AC, Luxon, AM, Todsen, AL, Tullett, AM, Kuzminska, AO, Krafnick, AJ, Askelund, AD, Charyate, AC, Wichman, AL, Thomas, AG, Forscher, PS, Mallik, PR, Coles, NA, Miller, JK, Urry, HL, IJzerman, H, Basnight-Brown, DM, Ebersole, CR, Chartier, CR, Buchanan, EM, and Primbs, MA

Abstract

Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This crosscountry, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e., a controlling message) compared with no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared with the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly internalized form of motivation relying on one's core values) or behavioral intentions. Results supported hypothesized associations between people's existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing. Controlled motivation was associated with more defiance and less long-term behavioral intention to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges.

Published
2022

3. Characterization of Enterobacter phage vB_EcRAM-01, a new Pseudotevenvirus against Enterobacter cloacae, isolated in an urban river in Panama.

Author

Victoria-Blanco EE, González-Gómez JP, Medina-Sánchez JR, Martínez AA, Castro Del Campo N, Chaidez-Quiroz C, Querol-Audi J, and Martínez-Torres AO

Subjects
Panama, Host Specificity, Phylogeny, Whole Genome Sequencing, Enterobacter cloacae virology, Enterobacter cloacae genetics, Rivers microbiology, Rivers virology, Myoviridae genetics, Myoviridae isolation & purification, Genome, Viral, Bacteriophages isolation & purification, Bacteriophages genetics
Abstract

The Enterobacter cloacae complex, a prominent bacterium responsible worldwide for most bloodstream infections in the hospital environment, has shown broad-spectrum antibiotic resistance, including carbapenems. Therefore, bacteriophages have again attracted the attention of the science and medical community as an alternative to control Multidrug resistant bacteria. In this study, water samples from Río Abajo River, in Panama City, Panama, were collected, for phage isolation, purification, characterization and propagation against the E. cloacae complex. As result, a phage produced clear and round plaque-forming units indicating a lytic phage was isolated. Further analyses concluded that this phage is stable at temperatures between 25°C and 50°C, it remains infective in a pH range between 7 to 11, with high sensitivity to Ultraviolet light. Remarkedly, it exhibits a narrow host specificity only infecting E. cloacae. Whole genome sequencing revealed that is a myovirus with a genome size of 178,477 bp, a G-C content of 45.8%, and containing approximately 294 genes. Among them, protein-encoding genes involved in morphology, inactivation, adsorption to cells, DNA injection and lytic enzymes were identified. Additionally, the genome contained two tRNA sequences. Genes that encode holins and endolysins, typical of lytic bacteriophages, were also present. A whole-genome sequencing analysis indicated that, according to the genus demarcation criteria, this phage belongs to a novel species within the Family Straboviridae, called genus Pseudotevenvirus., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Victoria-Blanco et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Published
2024
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4. Genomic insights of S. aureus associated with bovine mastitis in a high livestock activity region of Mexico.

Author

Aguirre-Sánchez JR, Castro-Del Campo N, Medrano-Félix JA, Martínez-Torres AO, Chaidez C, Querol-Audi J, and Castro-Del Campo N

Subjects
Animals, Cattle, Mexico epidemiology, Female, Virulence genetics, Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial genetics, Mastitis, Bovine microbiology, Staphylococcus aureus genetics, Staphylococcus aureus drug effects, Staphylococcus aureus pathogenicity, Staphylococcal Infections veterinary, Staphylococcal Infections microbiology, Staphylococcal Infections epidemiology, Genome, Bacterial
Abstract

Importance: Bovine mastitis, predominantly associated with gram-positive Staphylococcus aureus , poses a significant threat to dairy cows, leading to a decline in milk quality and volume with substantial economic implications., Objective: This study investigated the incidence, virulence, and antibiotic resistance of S. aureus associated with mastitis in dairy cows., Methods: Fifty milk-productive cows underwent a subclinical mastitis diagnosis, and the S. aureus strains were isolated. Genomic DNA extraction, sequencing, and bioinformatic analysis were performed, supplemented by including 124 S. aureus genomes from cows with subclinical mastitis to enhance the overall analysis., Results: The results revealed a 42% prevalence of subclinical mastitis among the cows tested. Genomic analysis identified 26 sequence types (STs) for all isolates, with Mexican STs belonging primarily to CC1 and CC97. The analyzed genomes exhibited multidrug resistance to phenicol, fluoroquinolone, tetracycline, and cephalosporine, which are commonly used as the first line of treatment. Furthermore, a similar genomic virulence repertoire was observed across the genomes, encompassing the genes related to invasion, survival, pathogenesis, and iron uptake. In particular, the toxic shock syndrome toxin ( tss-1 ) was found predominantly in the genomes isolated in this study, posing potential health risks, particularly in children., Conclusion and Relevance: These findings underscore the broad capacity for antibiotic resistance and pathogenicity by S. aureus , compromising the integrity of milk and dairy products. The study emphasizes the need to evaluate the effectiveness of antibiotics in combating S. aureus infections., Competing Interests: The authors declare no conflicts of interest., (© 2024 The Korean Society of Veterinary Science.)

Published
2024
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5. Prevalence of Plasmid-Associated Tetracycline Resistance Genes in Multidrug-Resistant Escherichia coli Strains Isolated from Environmental, Animal and Human Samples in Panama.

Author

Ramírez-Bayard IE, Mejía F, Medina-Sánchez JR, Cornejo-Reyes H, Castillo M, Querol-Audi J, and Martínez-Torres AO

Abstract

Antimicrobial resistance bacteria are nowadays ubiquitous. Its presence has been reported in almost every type of source, from water for agricultural and recreative use, water distribution pipes, and wastewater, to food, fomites, and clinical samples. Enterobacteriaceae, especially Escherichia coli , are not the exception, showing an increased resistance to several antibiotics, causing a global health and economic burden. Therefore, the monitoring of fecal microbiota is important because it is present in numerous reservoirs where gene transfer between commensal and virulent bacteria can take place, representing a potential source of resistant E. coli . In this work, antibiotic resistance profiles of 150 E. coli isolates from environmental, animal, and human samples, collected in three rural areas in Panama, were analyzed. A total of 116 isolates were resistant to at least one of the nine antibiotics tested. Remarkably, almost 100% of these exhibited resistance to tetracycline. Plasmid-associated tetA and tetB genes were detected in 42.86% of the isolates analyzed, tetA being the most prevalent. These results suggest that tetracycline resistance would be used as a convenient indicator of genetic horizontal transfer within a community.

Published
2023
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6. Author Correction: A multi-country test of brief reappraisal interventions on emotions during the COVID-19 pandemic.

Author

Wang K, Goldenberg A, Dorison CA, Miller JK, Uusberg A, Lerner JS, Gross JJ, Agesin BB, Bernardo M, Campos O, Eudave L, Grzech K, Ozery DH, Jackson EA, Garcia EOL, Drexler SM, Jurković AP, Rana K, Wilson JP, Antoniadi M, Desai K, Gialitaki Z, Kushnir E, Nadif K, Bravo ON, Nauman R, Oosterlinck M, Pantazi M, Pilecka N, Szabelska A, van Steenkiste IMM, Filip K, Bozdoc AI, Marcu GM, Agadullina E, Adamkovič M, Roczniewska M, Reyna C, Kassianos AP, Westerlund M, Ahlgren L, Pöntinen S, Adetula GA, Dursun P, Arinze AI, Arinze NC, Ogbonnaya CE, Ndukaihe ILG, Dalgar I, Akkas H, Macapagal PM, Lewis S, Metin-Orta I, Foroni F, Willis M, Santos AC, Mokady A, Reggev N, Kurfali MA, Vasilev MR, Nock NL, Parzuchowski M, Espinoza Barría MF, Vranka M, Kohlová MB, Ropovik I, Harutyunyan M, Wang C, Yao E, Becker M, Manunta E, Kaminski G, Boudesseul J, Marko D, Evans K, Lewis DMG, Findor A, Landry AT, Aruta JJB, Ortiz MS, Vally Z, Pronizius E, Voracek M, Lamm C, Grinberg M, Li R, Valentova JV, Mioni G, Cellini N, Chen SC, Zickfeld J, Moon K, Azab H, Levy N, Karababa A, Beaudry JL, Boucher L, Collins WM, Todsen AL, van Schie K, Vintr J, Bavolar J, Kaliska L, Križanić V, Samojlenko L, Pourafshari R, Geiger SJ, Beitner J, Warmelink L, Ross RM, Stephen ID, Hostler TJ, Azouaghe S, McCarthy R, Szala A, Grano C, Solorzano CS, Anjum G, Jimenez-Leal W, Bradford M, Pérez LC, Cruz Vásquez JE, Galindo-Caballero OJ, Vargas-Nieto JC, Kácha O, Arvanitis A, Xiao Q, Cárcamo R, Zorjan S, Tajchman Z, Vilares I, Pavlacic JM, Kunst JR, Tamnes CK, von Bastian CC, Atari M, Sharifian M, Hricova M, Kačmár P, Schrötter J, Rahal RM, Cohen N, FatahModares S, Zrimsek M, Zakharov I, Koehn MA, Esteban-Serna C, Calin-Jageman RJ, Krafnick AJ, Štrukelj E, Isager PM, Urban J, Silva JR, Martončik M, Očovaj SB, Šakan D, Kuzminska AO, Djordjevic JM, Almeida IAT, Ferreira A, Lazarevic LB, Manley H, Ricaurte DZ, Monteiro RP, Etabari Z, Musser E, Dunleavy D, Chou W, Godbersen H, Ruiz-Fernández S, Reeck C, Batres C, Kirgizova K, Muminov A, Azevedo F, Alvarez DS, Butt MM, Lee JM, Chen Z, Verbruggen F, Ziano I, Tümer M, Charyate ACA, Dubrov D, Tejada Rivera MDCMC, Aberson C, Pálfi B, Maldonado MA, Hubena B, Sacakli A, Ceary CD, Richard KL, Singer G, Perillo JT, Ballantyne T, Cyrus-Lai W, Fedotov M, Du H, Wielgus M, Pit IL, Hruška M, Sousa D, Aczel B, Hajdu N, Szaszi B, Adamus S, Barzykowski K, Micheli L, Schmidt ND, Zsido AN, Paruzel-Czachura M, Muda R, Bialek M, Kowal M, Sorokowska A, Misiak M, Mola D, Ortiz MV, Correa PS, Belaus A, Muchembled F, Ribeiro RR, Arriaga P, Oliveira R, Vaughn LA, Szwed P, Kossowska M, Czarnek G, Kielińska J, Antazo B, Betlehem R, Stieger S, Nilsonne G, Simonovic N, Taber J, Gourdon-Kanhukamwe A, Domurat A, Ihaya K, Yamada Y, Urooj A, Gill T, Čadek M, Bylinina L, Messerschmidt J, Kurfalı M, Adetula A, Baklanova E, Albayrak-Aydemir N, Kappes HB, Gjoneska B, House T, Jones MV, Berkessel JB, Chopik WJ, Çoksan S, Seehuus M, Khaoudi A, Bokkour A, El Arabi KA, Djamai I, Iyer A, Parashar N, Adiguzel A, Kocalar HE, Bundt C, Norton JO, Papadatou-Pastou M, De la Rosa-Gomez A, Ankushev V, Bogatyreva N, Grigoryev D, Ivanov A, Prusova I, Romanova M, Sarieva I, Terskova M, Hristova E, Kadreva VH, Janak A, Schei V, Sverdrup TE, Askelund AD, Pineda LMS, Krupić D, Levitan CA, Johannes N, Ouherrou N, Say N, Sinkolova S, Janjić K, Stojanovska M, Stojanovska D, Khosla M, Thomas AG, Kung FYH, Bijlstra G, Mosannenzadeh F, Balci BB, Reips UD, Baskin E, Ishkhanyan B, Czamanski-Cohen J, Dixson BJW, Moreau D, Sutherland CAM, Chuan-Peng H, Noone C, Flowe H, Anne M, Janssen SMJ, Topor M, Majeed NM, Kunisato Y, Yu K, Daches S, Hartanto A, Vdovic M, Anton-Boicuk L, Forbes PAG, Kamburidis J, Marinova E, Nedelcheva-Datsova M, Rachev NR, Stoyanova A, Schmidt K, Suchow JW, Koptjevskaja-Tamm M, Jernsäther T, Olofsson JK, Bialobrzeska O, Marszalek M, Tatachari S, Afhami R, Law W, Antfolk J, Žuro B, Van Doren N, Soto JA, Searston R, Miranda J, Damnjanović K, Yeung SK, Krupić D, Hoyer K, Jaeger B, Ren D, Pfuhl G, Klevjer K, Corral-Frías NS, Frias-Armenta M, Lucas MY, Torres AO, Toro M, Delgado LGJ, Vega D, Solas SÁ, Vilar R, Massoni S, Frizzo T, Bran A, Vaidis DC, Vieira L, Paris B, Capizzi M, Coelho GLH, Greenburgh A, Whitt CM, Tullett AM, Du X, Volz L, Bosma MJ, Karaarslan C, Sarıoğuz E, Allred TB, Korbmacher M, Colloff MF, Lima TJS, Ribeiro MFF, Verharen JPH, Karekla M, Karashiali C, Sunami N, Jaremka LM, Storage D, Habib S, Studzinska A, Hanel PHP, Holford DL, Sirota M, Wolfe K, Chiu F, Theodoropoulou A, Ahn ER, Lin Y, Westgate EC, Brohmer H, Hofer G, Dujols O, Vezirian K, Feldman G, Travaglino GA, Ahmed A, Li M, Bosch J, Torunsky N, Bai H, Manavalan M, Song X, Walczak RB, Zdybek P, Friedemann M, Rosa AD, Kozma L, Alves SG, Lins S, Pinto IR, Correia RC, Babinčák P, Banik G, Rojas-Berscia LM, Varella MAC, Uttley J, Beshears JE, Thommesen KK, Behzadnia B, Geniole SN, Silan MA, Maturan PLG, Vilsmeier JK, Tran US, Izquierdo SM, Mensink MC, Sorokowski P, Groyecka-Bernard A, Radtke T, Adoric VC, Carpentier J, Özdoğru AA, Joy-Gaba JA, Hedgebeth MV, Ishii T, Wichman AL, Röer JP, Ostermann T, Davis WE, Suter L, Papachristopoulos K, Zabel C, Onie S, Ebersole CR, Chartier CR, Mallik PR, Urry HL, Buchanan EM, Coles NA, Primbs MA, Basnight-Brown DM, IJzerman H, Forscher PS, and Moshontz H

Published
2022
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7. Complete Genome Sequence of Enterobacter Phage vB_EcRAM-01, a New Pseudotevenvirus against the Enterobacter cloacae Complex.

Author

Victoria-Blanco EE, González-Gómez JP, Quiroz E, Martínez AA, González C, Castro Del Campo N, Chaidez-Quiroz C, Querol-Audi J, and Martínez-Torres AO

Abstract

Here, we present the complete genome sequence of Enterobacter phage vB_EcRAM-01, isolated from waters of the Río Abajo river, in Panama City, Panama. This phage has deployed lytic activity against the Enterobacter cloacae complex, a pathogen of clinical importance in intensive care units. It belongs to the Myoviridae family and has a double-stranded DNA genome that is 178,477 bp long and contains 293 open reading frames (ORFs).

Published
2022
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8. A multi-country test of brief reappraisal interventions on emotions during the COVID-19 pandemic.

Author

Wang K, Goldenberg A, Dorison CA, Miller JK, Uusberg A, Lerner JS, Gross JJ, Agesin BB, Bernardo M, Campos O, Eudave L, Grzech K, Ozery DH, Jackson EA, Garcia EOL, Drexler SM, Jurković AP, Rana K, Wilson JP, Antoniadi M, Desai K, Gialitaki Z, Kushnir E, Nadif K, Bravo ON, Nauman R, Oosterlinck M, Pantazi M, Pilecka N, Szabelska A, van Steenkiste IMM, Filip K, Bozdoc AI, Marcu GM, Agadullina E, Adamkovič M, Roczniewska M, Reyna C, Kassianos AP, Westerlund M, Ahlgren L, Pöntinen S, Adetula GA, Dursun P, Arinze AI, Arinze NC, Ogbonnaya CE, Ndukaihe ILG, Dalgar I, Akkas H, Macapagal PM, Lewis S, Metin-Orta I, Foroni F, Willis M, Santos AC, Mokady A, Reggev N, Kurfali MA, Vasilev MR, Nock NL, Parzuchowski M, Espinoza Barría MF, Vranka M, Kohlová MB, Ropovik I, Harutyunyan M, Wang C, Yao E, Becker M, Manunta E, Kaminski G, Boudesseu J, Marko D, Evans K, Lewis DMG, Findor A, Landry AT, Aruta JJB, Ortiz MS, Vally Z, Pronizius E, Voracek M, Lamm C, Grinberg M, Li R, Valentova JV, Mioni G, Cellini N, Chen SC, Zickfeld J, Moon K, Azab H, Levy N, Karababa A, Beaudry JL, Boucher L, Collins WM, Todsen AL, van Schie K, Vintr J, Bavolar J, Kaliska L, Križanić V, Samojlenko L, Pourafshari R, Geiger SJ, Beitner J, Warmelink L, Ross RM, Stephen ID, Hostler TJ, Azouaghe S, McCarthy R, Szala A, Grano C, Solorzano CS, Anjum G, Jimenez-Leal W, Bradford M, Pérez LC, Cruz Vásquez JE, Galindo-Caballero OJ, Vargas-Nieto JC, Kácha O, Arvanitis A, Xiao Q, Cárcamo R, Zorjan S, Tajchman Z, Vilares I, Pavlacic JM, Kunst JR, Tamnes CK, von Bastian CC, Atari M, Sharifian M, Hricova M, Kačmár P, Schrötter J, Rahal RM, Cohen N, FatahModares S, Zrimsek M, Zakharov I, Koehn MA, Esteban-Serna C, Calin-Jageman RJ, Krafnick AJ, Štrukelj E, Isager PM, Urban J, Silva JR, Martončik M, Očovaj SB, Šakan D, Kuzminska AO, Djordjevic JM, Almeida IAT, Ferreira A, Lazarevic LB, Manley H, Ricaurte DZ, Monteiro RP, Etabari Z, Musser E, Dunleavy D, Chou W, Godbersen H, Ruiz-Fernández S, Reeck C, Batres C, Kirgizova K, Muminov A, Azevedo F, Alvarez DS, Butt MM, Lee JM, Chen Z, Verbruggen F, Ziano I, Tümer M, Charyate ACA, Dubrov D, Tejada Rivera MDCMC, Aberson C, Pálfi B, Maldonado MA, Hubena B, Sacakli A, Ceary CD, Richard KL, Singer G, Perillo JT, Ballantyne T, Cyrus-Lai W, Fedotov M, Du H, Wielgus M, Pit IL, Hruška M, Sousa D, Aczel B, Hajdu N, Szaszi B, Adamus S, Barzykowski K, Micheli L, Schmidt ND, Zsido AN, Paruzel-Czachura M, Muda R, Bialek M, Kowal M, Sorokowska A, Misiak M, Mola D, Ortiz MV, Correa PS, Belaus A, Muchembled F, Ribeiro RR, Arriaga P, Oliveira R, Vaughn LA, Szwed P, Kossowska M, Czarnek G, Kielińska J, Antazo B, Betlehem R, Stieger S, Nilsonne G, Simonovic N, Taber J, Gourdon-Kanhukamwe A, Domurat A, Ihaya K, Yamada Y, Urooj A, Gill T, Čadek M, Bylinina L, Messerschmidt J, Kurfalı M, Adetula A, Baklanova E, Albayrak-Aydemir N, Kappes HB, Gjoneska B, House T, Jones MV, Berkessel JB, Chopik WJ, Çoksan S, Seehuus M, Khaoudi A, Bokkour A, El Arabi KA, Djamai I, Iyer A, Parashar N, Adiguzel A, Kocalar HE, Bundt C, Norton JO, Papadatou-Pastou M, De la Rosa-Gomez A, Ankushev V, Bogatyreva N, Grigoryev D, Ivanov A, Prusova I, Romanova M, Sarieva I, Terskova M, Hristova E, Kadreva VH, Janak A, Schei V, Sverdrup TE, Askelund AD, Pineda LMS, Krupić D, Levitan CA, Johannes N, Ouherrou N, Say N, Sinkolova S, Janjić K, Stojanovska M, Stojanovska D, Khosla M, Thomas AG, Kung FYH, Bijlstra G, Mosannenzadeh F, Balci BB, Reips UD, Baskin E, Ishkhanyan B, Czamanski-Cohen J, Dixson BJW, Moreau D, Sutherland CAM, Chuan-Peng H, Noone C, Flowe H, Anne M, Janssen SMJ, Topor M, Majeed NM, Kunisato Y, Yu K, Daches S, Hartanto A, Vdovic M, Anton-Boicuk L, Forbes PAG, Kamburidis J, Marinova E, Nedelcheva-Datsova M, Rachev NR, Stoyanova A, Schmidt K, Suchow JW, Koptjevskaja-Tamm M, Jernsäther T, Olofsson JK, Bialobrzeska O, Marszalek M, Tatachari S, Afhami R, Law W, Antfolk J, Žuro B, Van Doren N, Soto JA, Searston R, Miranda J, Damnjanović K, Yeung SK, Krupić D, Hoyer K, Jaeger B, Ren D, Pfuhl G, Klevjer K, Corral-Frías NS, Frias-Armenta M, Lucas MY, Torres AO, Toro M, Delgado LGJ, Vega D, Solas SÁ, Vilar R, Massoni S, Frizzo T, Bran A, Vaidis DC, Vieira L, Paris B, Capizzi M, Coelho GLH, Greenburgh A, Whitt CM, Tullett AM, Du X, Volz L, Bosma MJ, Karaarslan C, Sarıoğuz E, Allred TB, Korbmacher M, Colloff MF, Lima TJS, Ribeiro MFF, Verharen JPH, Karekla M, Karashiali C, Sunami N, Jaremka LM, Storage D, Habib S, Studzinska A, Hanel PHP, Holford DL, Sirota M, Wolfe K, Chiu F, Theodoropoulou A, Ahn ER, Lin Y, Westgate EC, Brohmer H, Hofer G, Dujols O, Vezirian K, Feldman G, Travaglino GA, Ahmed A, Li M, Bosch J, Torunsky N, Bai H, Manavalan M, Song X, Walczak RB, Zdybek P, Friedemann M, Rosa AD, Kozma L, Alves SG, Lins S, Pinto IR, Correia RC, Babinčák P, Banik G, Rojas-Berscia LM, Varella MAC, Uttley J, Beshears JE, Thommesen KK, Behzadnia B, Geniole SN, Silan MA, Maturan PLG, Vilsmeier JK, Tran US, Izquierdo SM, Mensink MC, Sorokowski P, Groyecka-Bernard A, Radtke T, Adoric VC, Carpentier J, Özdoğru AA, Joy-Gaba JA, Hedgebeth MV, Ishii T, Wichman AL, Röer JP, Ostermann T, Davis WE, Suter L, Papachristopoulos K, Zabel C, Onie S, Ebersole CR, Chartier CR, Mallik PR, Urry HL, Buchanan EM, Coles NA, Primbs MA, Basnight-Brown DM, IJzerman H, Forscher PS, and Moshontz H

Subjects
Adult, Female, Humans, Male, COVID-19 psychology, Emotional Regulation, Emotions
Abstract

The COVID-19 pandemic has increased negative emotions and decreased positive emotions globally. Left unchecked, these emotional changes might have a wide array of adverse impacts. To reduce negative emotions and increase positive emotions, we tested the effectiveness of reappraisal, an emotion-regulation strategy that modifies how one thinks about a situation. Participants from 87 countries and regions (n = 21,644) were randomly assigned to one of two brief reappraisal interventions (reconstrual or repurposing) or one of two control conditions (active or passive). Results revealed that both reappraisal interventions (vesus both control conditions) consistently reduced negative emotions and increased positive emotions across different measures. Reconstrual and repurposing interventions had similar effects. Importantly, planned exploratory analyses indicated that reappraisal interventions did not reduce intentions to practice preventive health behaviours. The findings demonstrate the viability of creating scalable, low-cost interventions for use around the world. PROTOCOL REGISTRATION: The stage 1 protocol for this Registered Report was accepted in principle on 12 May 2020. The protocol, as accepted by the journal, can be found at https://doi.org/10.6084/m9.figshare.c.4878591.v1., (© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)

Published
2021
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9. Molecular Epidemiology of Dengue in Panama: 25 Years of Circulation.

Author

Díaz Y, Chen-Germán M, Quiroz E, Carrera JP, Cisneros J, Moreno B, Cerezo L, Martinez-Torres AO, Moreno L, Barahona de Mosca I, Armién B, Chen R, Vasilakis N, and López-Vergès S

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Dengue epidemiology, Dengue Virus classification, Dengue Virus genetics, Female, Genotype, Humans, Infant, Male, Middle Aged, Molecular Epidemiology, Panama epidemiology, Phylogeny, Retrospective Studies, Young Adult, Dengue virology, Dengue Virus isolation & purification
Abstract

Dengue virus (DENV) is the most prevalent arbovirus in terms of human public health importance globally. In addition to DENV epidemiological surveillance, genomic surveillance may help investigators understand the epidemiological dynamics, geographic distribution, and temporal patterns of DENV circulation. Herein, we aimed to reconstruct the molecular epidemiology and phylogeny of DENV in Panama to connect the epidemiological history of DENV dispersal and circulation in Latin America. We retrospectively analyzed the epidemiological data obtained during 25 years of DENV surveillance in Panama. DENV was reintroduced in Panama in 1993 after a 35 year absence of autochthonous transmission. The increase in the number of total dengue cases has been accompanied by an increase in severe and fatal cases, with the highest case fatality rate recorded in 2011. All four serotypes were detected in Panama, which is characterized by serotype replacement and/or co-circulation of multiple serotypes. Phylogenetic analysis of datasets collected from envelope (E) gene sequences obtained from viruses isolated from human sera demonstrated that circulating viruses were highly diverse and clustered in distinct clades, with co-circulation of clades from the same genotype. Our analyses also suggest that Panamanian strains were related to viruses from different regions of the Americas, suggesting a continuous exchange of viruses within the Americas.

Published
2019
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10. Molecular Characterization of Anaplasma sp. in Free-Living Gray Brockets (Mazama gouazoubira).

Author

Mongruel AC, Benevenute JL, André MR, Carrasco AO, Machado RZ, and Seki MC

Subjects
Anaplasma classification, Anaplasmosis epidemiology, Animals, Bacterial Proteins genetics, Bacterial Proteins metabolism, Brazil epidemiology, Gene Expression Regulation, Bacterial physiology, Genotype, Phylogeny, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Anaplasma genetics, Anaplasmosis parasitology, Deer parasitology
Abstract

Bacteria in the genus Anaplasma are responsible for diseases in animals and humans. Studies carried out in Brazil have demonstrated that Brazilian deer are able to act as hosts of agents in the family Anaplasmataceae and are possibly potential reservoirs of these pathogens. Molecular and phylogenetic studies have been carried out on samples of two gray brocket specimens (Mazama gouazoubira) from the city of Guarapuava, state of Paraná, Brazil, for the detection of Anaplasma sp. in these animals. Partial nucleotide sequences of the genes 16S rRNA and groESL were used for phylogenetic analyses and compared with other 13 and 17 partial sequences of the respective genes obtained in GenBank. These assessments showed topological incongruence among the trees generated in the phylogenetic analyses. Phylogenetic analysis based on the gene 16S rRNA of the genotypes amplified in the samples of this study was similar to those of A. bovis detected in dogs and wild deer in Japan, whereas studies carried out on gene groESL indicated proximity with sequences of Anaplasma sp. that were also isolated in deer in Japan and allocated in the same clade of partial sequences of A. phagocytophilum. As the 16S rRNA gene is highly conserved, with few polymorphic positions, it may show low reliability for studies on phylogenetic positioning. The present study detected an Anaplasma sp. genotype in two specimens of M. gouazoubira in southern Brazil, which may mean that this agent possibly circulates in deer populations, and demonstrated the need for studies related to the possible role of deer in enzootic cycles of Anaplasmataceae in Brazil.

Published
2017
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11. Genetic Characterization of Rubella Virus Strains Detected in Spain, 1998-2014.

Author

Martínez-Torres AO, Mosquera MM, De Ory F, González-Praetorius A, and Echevarría JE

Subjects
Disease Outbreaks, Genotype, Humans, Phylogeny, Rubella virus classification, Rubella virus isolation & purification, Spain epidemiology, Rubella epidemiology, Rubella virology, Rubella virus genetics
Abstract

The National Plan for the Elimination of Rubella was implemented in Spain in 2008 using the logistics of the National Plan for the Elimination of Measles that have been employed since year 2000. Molecular characterization of rubella virus (RUBV) is important for disease surveillance and for monitoring elimination of the disease throughout the world. We describe the first complete series of data regarding the circulation of RUBV genotypes in Spain. The 739-nucleotide fragment designated by the WHO for RUBV genotyping was sequenced in 88 selected cases collected from 1998 to 2014. Five genotypes were identified: 1E, 2B, 1J, 1I, and 1a. Genotype 1E was predominant between 1998 and 2003 but was replaced by genotype 2B, which was detected in sporadic cases in 2004, 2006, 2008, 2012, 2013 and 2014. There was an outbreak of genotype 2B in Algeciras (Andalusia) in 2008. Genotype 1J caused an outbreak in Madrid in 2004/2005 and sporadic cases in 2005 and 2007. Genotype 1I was found to have infected an immune-suppressed patient with neurological symptoms in 2008. Finally, vaccine strain RA 27/3 was detected in three sporadic cases, two of them immune-suppressed and without a recent history of vaccination. This suggests that during these years there were a series of imported sporadic cases and outbreaks, confirming the findings of epidemiological data analysis. The importation sources were generally consistent with our geographic and cultural ties, mainly with Europe (genotypes 1E, 2B, 1I) and Latin America (1J)., Competing Interests: The authors have declared that no competing interests exist.

Published
2016
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12. Madariaga virus infection associated with a case of acute disseminated encephalomyelitis.

Author

Luciani K, Abadía I, Martínez-Torres AO, Cisneros J, Guerra I, García M, Estripeaut D, and Carrera JP

Subjects
Alphavirus, Alphavirus Infections diagnosis, Alphavirus Infections epidemiology, Alphavirus Infections pathology, Brain pathology, Child, Encephalomyelitis, Acute Disseminated diagnosis, Encephalomyelitis, Acute Disseminated pathology, Encephalomyelitis, Acute Disseminated virology, Humans, Magnetic Resonance Imaging, Male, Neuroimaging, Panama epidemiology, Alphavirus Infections complications, Encephalomyelitis, Acute Disseminated etiology
Abstract

We present the first report of a pediatric case of acute disseminated encephalomyelitis (ADEM) associated with Madariaga virus infection (MADV, Alphavirus, Togaviridae; formerly known as South American variants of eastern equine encephalitis virus [EEEV]) in a patient of the 2010 alphaviral epidemic reported in Panama. The patient was admitted to the Hospital del Niño in Panama City with suspected meningitis, exhibited with decreased alertness and disorientation in space and time, hemiparesis, and left Babinski sign. The patient was transferred to the intensive care unit and treated with aciclovir and methylprednisolone. The magnetic resonance imaging (MRI) of the brain revealed multiple hyperintense lesions at T2-weighted images (T2) and fluid-attenuated inversion recovery (FLAIR) on the cortical-subcortical level. Sera samples obtained on days 6 and 12 were immunoglobulin M (IgM) positive for MADV. The findings on the clinical and cerebrospinal analyses, rapid symptom progression as well as neuroimaging, and serologic studies support our diagnosis. Our results suggest that MADV should be included in the etiologic differential diagnosis of ADEM in endemic countries., (© The American Society of Tropical Medicine and Hygiene.)

Published
2015
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13. Eastern equine encephalitis in Latin America.

Author

Carrera JP, Forrester N, Wang E, Vittor AY, Haddow AD, López-Vergès S, Abadía I, Castaño E, Sosa N, Báez C, Estripeaut D, Díaz Y, Beltrán D, Cisneros J, Cedeño HG, Travassos da Rosa AP, Hernandez H, Martínez-Torres AO, Tesh RB, and Weaver SC

Subjects
Adolescent, Animals, Antibodies, Viral blood, Child, Child, Preschool, Fatal Outcome, Female, Horse Diseases epidemiology, Horses, Humans, Infant, Male, Panama epidemiology, Phylogeny, RNA, Viral blood, Disease Outbreaks, Encephalitis Virus, Eastern Equine genetics, Encephalitis Virus, Eastern Equine immunology, Encephalitis Virus, Eastern Equine isolation & purification, Encephalitis Virus, Venezuelan Equine genetics, Encephalitis Virus, Venezuelan Equine immunology, Encephalitis Virus, Venezuelan Equine isolation & purification, Encephalomyelitis, Eastern Equine epidemiology, Encephalomyelitis, Eastern Equine veterinary, Encephalomyelitis, Venezuelan Equine epidemiology, Encephalomyelitis, Venezuelan Equine veterinary
Abstract

Background: The eastern equine encephalitis (EEE) and Venezuelan equine encephalitis (VEE) viruses are pathogens that infect humans and horses in the Americas. Outbreaks of neurologic disease in humans and horses were reported in Panama from May through early August 2010., Methods: We performed antibody assays and tests to detect viral RNA and isolate the viruses in serum samples from hospitalized patients. Additional cases were identified with enhanced surveillance., Results: A total of 19 patients were hospitalized for encephalitis. Among them, 7 had confirmed EEE, 3 had VEE, and 1 was infected with both viruses; 3 patients died, 1 of whom had confirmed VEE. The clinical findings for patients with EEE included brain lesions, seizures that evolved to status epilepticus, and neurologic sequelae. An additional 99 suspected or probable cases of alphavirus infection were detected during active surveillance. In total, 13 cases were confirmed as EEE, along with 11 cases of VEE and 1 case of dual infection. A total of 50 cases in horses were confirmed as EEE and 8 as VEE; mixed etiologic factors were associated with 11 cases in horses. Phylogenetic analyses of isolates from 2 cases of equine infection with the EEE virus and 1 case of human infection with the VEE virus indicated that the viruses were of enzootic lineages previously identified in Panama rather than new introductions., Conclusions: Cases of EEE in humans in Latin America may be the result of ecologic changes that increased human contact with enzootic transmission cycles, genetic changes in EEE viral strains that resulted in increased human virulence, or an altered host range. (Funded by the National Institutes of Health and the Secretaría Nacional de Ciencia, Tecnología e Innovación, Panama.).

Published
2013
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14. Chlamydophila psittaci infections in hyacinth macaws (Anodorhynchus hyacinthinus) confiscated in Brazil.

Author

Raso TF, Teixeira RH, Carrasco AO, Araújo JP Jr, and Pinto AA

Subjects
Animals, Antibodies, Bacterial blood, Brazil epidemiology, Commerce, Conservation of Natural Resources, Crime, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Psittacosis epidemiology, Psittacosis microbiology, Bird Diseases microbiology, Chlamydophila psittaci isolation & purification, Psittaciformes, Psittacosis veterinary
Abstract

The hyacinth macaw (Anodorhynchus hyacinthinus) is the largest species of psittacine birds. It is considered endangered and illegal trade is one of the main factors involved in its decline. In this study, 26 hyacinth macaws maintained under poor husbandry conditions and destined for the illegal trade were confiscated in São Paulo State, Brazil. These birds were evaluated for the presence of antibodies against Chlamydophila psittaci by complement fixation test and C. psittaci DNA by seminested polymerase chain reaction. Results showed that 65.4% of the macaws were positive for at least one test. Birds with subclinical infections can shed chlamydiae intermittently over long periods, contributing to the dissemination of the agent. Global trade is one of the most important drivers of disease emergence. The high percentage of positive samples in this study emphasizes the potential risk that the illegal trade of wild birds represents for both human and animal health.

Published
2013
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15. Prevalence of Salmonella spp. antibodies to Toxoplasma gondii, and Newcastle disease virus in feral pigeons (Columba livia) in the city of Jaboticabal, Brazil.

Author

de Sousa E, Berchieri AJ, Pinto AA, Machado RZ, de Carrasco AO, Marciano JA, and Werther K

Subjects
Animals, Bird Diseases blood, Bird Diseases epidemiology, Brazil epidemiology, Newcastle Disease immunology, Newcastle Disease virology, Newcastle disease virus immunology, Salmonella immunology, Salmonella Infections, Animal immunology, Salmonella Infections, Animal virology, Toxoplasma immunology, Toxoplasmosis, Animal immunology, Antibodies blood, Bird Diseases immunology, Columbidae, Newcastle Disease epidemiology, Salmonella Infections, Animal epidemiology, Toxoplasmosis, Animal epidemiology
Abstract

The rock pigeon (Columba livia) may serve as a reservoir for several pathogenic agents that can be transmitted to poultry, wildlife, domesticated pets, and/or humans via excreta, secretions, or dust from feathers. In addition, ingestion of infected pigeons by wild and domestic animals can also transmit these pathogenic agents. The health status of 126 free-living pigeons in an urban area was evaluated by microbiologic culture for Salmonella and serologic testing for the presence of antibodies for Toxoplasma gondii and for Newcastle disease virus (NDV) from 120 and 109 pigeons, respectively. After drawing blood, the birds were euthanized, and fragments of the liver, spleen, lungs, and gonads, and feces were cultured for Salmonella spp. Salmonella spp. was isolated from 10 birds (7.94%), of which 8 were Salmonella typhimurium, one was Salmonella enterica subsp. enterica serotype 4,12 and one was Salmonella enterica subsp. enterica serotype 4,12,i. Six of 109 pigeons (5.50%) were positive for NDV antibodies when using the hemagglutination inhibition test. Toxoplasma gondii antibodies were detected by immunofluorescence in one of 120 sera tested (0.83%). The results indicate that feral rock pigeons were exposed to NDV and T. gondii, although the exposure was low. In addition, these birds had Salmonella spp. and could disseminate this pathogen in the environment.

Published
2010
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16. Search for Salmonella spp. in ostrich productive chain of Brazilian southeast region.

Author

de Freitas Neto OC, Lages SL, Carrasco AO, and Berchieri Junior A

Subjects
Abattoirs, Animal Feed microbiology, Animals, Bird Diseases epidemiology, Brazil epidemiology, Feces microbiology, Gastrointestinal Contents microbiology, Liver microbiology, Ovum microbiology, Rodentia, Salmonella classification, Salmonella Infections, Animal microbiology, Spleen microbiology, Struthioniformes, Bird Diseases microbiology, Salmonella isolation & purification, Salmonella Infections, Animal epidemiology
Abstract

We analyzed ostriches from an equipped farm located in the Brazilian southeast region for the presence of Salmonella spp. This bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings from rodents. Additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver and carcasses from 90 slaughtered ostriches from the studied farm. Also, blood serum of those animals were harvested and submitted to serum plate agglutination using commercial Salmonella Pullorum antigen. No Salmonella spp. was detected in any eggs, caecal content, liver, spleen, carcass and droppings from ostriches and rodents. However, Salmonella Javiana and Salmonella enterica subsp. enterica 4, 12: i:- were isolated from some samples of feed. The serologic test was negative for all samples. Good sanitary farming management and the application of HACCP principles and GMP during the slaughtering process could explain the absence of Salmonella spp. in the tested samples.

Published
2009
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17. Phylogenetic analysis of rubella virus strains from an outbreak in Madrid, Spain, from 2004 to 2005.

Author

Martínez-Torres AO, Mosquera MM, Sanz JC, Ramos B, and Echevarría JE

Subjects
Epitopes genetics, Epitopes immunology, Humans, Male, Molecular Sequence Data, Mutation, Missense, RNA, Viral genetics, Rubella virus isolation & purification, Sequence Analysis, DNA, Sequence Homology, Spain epidemiology, Viral Structural Proteins genetics, Viral Structural Proteins immunology, Disease Outbreaks, Phylogeny, Rubella epidemiology, Rubella virology, Rubella virus classification, Rubella virus genetics
Abstract

An outbreak of rubella affected 460 individuals in 2004 and 2005 in the community of Madrid, Spain. Most of the patients were nonvaccinated Latin American immigrants or Spanish males. This study presents the first data on rubella virus genotypes in Spain. Forty selected clinical samples (2 urine, 5 serum, 3 blood, 2 saliva, and 28 pharyngeal exudate samples) from 40 cases were collected. The 739-nucleotide sequence recommended by the World Health Organization obtained from viral RNA in these samples was analyzed by using the MEGA v4.0 software. Seventeen isolates were obtained from 40 clinical samples from the outbreak, including two isolated from congenital rubella syndrome cases. Only viral RNA of genotype 1j was detected in both isolates and clinical specimens. Two variations in amino acids, G253C and T394S, which are involved in neutralization epitopes arose during the outbreak, but apparently there was no positive selection of either of them. The origin of the outbreak remains unknown because of poor virologic surveillance in Latin America and the African countries neighboring Spain. On the other hand, this is the first report of this genotype in Europe. The few published sequences of genotype 1j indicate that it comes from Japan and the Philippines, but there are no epidemiological data supporting this as the origin of the Madrid outbreak.

Published
2009
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