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318 results on '"Tosi, Umberto"'

8. Mechanobiological Strategies to Enhance Ovine (Ovis aries) Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications.

Author

Haidar-Montes, Arlette A., Mauro, Annunziata, El Khatib, Mohammad, Prencipe, Giuseppe, Pierdomenico, Laura, Tosi, Umberto, Wouters, Guy, Cerveró-Varona, Adrián, Berardinelli, Paolo, Russo, Valentina, and Barboni, Barbara

Subjects
BIOMIMETIC materials, TISSUE differentiation, MESENCHYMAL stem cells, SHEEP, FAT cells
Abstract

Simple Summary: Tendon injuries are known to be difficult to heal, making effective treatments crucial. This study examines the potential of stem cells derived from sheep fat tissue for tendon repair, since little information is available on their tenogenic potential. The focus of this study was to understand how these stem cells can be induced to develop into tendon cells. Initially, the cells were grown in the lab to ensure that their essential properties were maintained. Two methods were then employed to promote the acquisition of tendon-like characteristics: exposing the cells to a tenogenic conditioned medium or seeding them on a scaffold that mimics a tendon structure. The results indicated that these stem cells could be expanded without aging or changes in their characteristics. When exposed to the tendon medium, the cells were able to start the tendon differentiation process but were not able to complete it. Conversely, when seeded on the scaffold, the cells changed their naïve genetic and protein profile and acquired that of the typical tendon cells. This suggests that these stem cells can be addressed towards tendon cells, being more effective when seeded on a tendon biomimetic scaffold. These findings highlight the potential use of these stem cell treatments for tendon injuries, particularly in veterinary medicine. Adipose-derived stem cells (ADSCs) hold promise for tendon repair, even if their tenogenic plasticity and underlying mechanisms remain only partially understood, particularly in cells derived from the ovine animal model. This study aimed to characterize oADSCs during in vitro expansion to validate their phenotypic properties pre-transplantation. Moreover, their tenogenic potential was assessed using two in vitro-validated approaches: (1) teno-inductive conditioned media (CM) derived from a co-culture between ovine amniotic stem cells and fetal tendon explants, and (2) short- (48 h) and long-term (14 days) seeding on highly aligned PLGA (ha-PLGA) electrospun scaffold. Our findings indicate that oADSCs can be expanded without senescence and can maintain the expression of stemness (Sox2, Oct4, Nanog) and mesenchymal (CD29, CD166, CD44, CD90) markers while remaining negative for hematopoietic (CD31, CD45) and MHC-II antigens. Of note, oADSCs' tendon differentiation potential greatly depended on the in vitro strategy. oADSCs exposed to CM significantly upregulated tendon-related genes (COL1, TNMD, THBS4) but failed to accumulate TNMD protein at 14 days of culture. Conversely, oADSCs seeded on ha-PLGA fleeces quickly upregulated the tendon-related genes (48 h) and in 14 days accumulated high levels of the TNMD protein into the cytoplasm of ADSCs, displaying a tenocyte-like morphology. This mechano-sensing cellular response involved a complete SOX9 downregulation accompanied by YAP activation, highlighting the efficacy of biophysical stimuli in promoting tenogenic differentiation. These findings underscore oADSCs' long-term self-renewal and tendon differentiative potential, thus opening their use in a preclinical setting to develop innovative stem cell-based and tissue engineering protocols for tendon regeneration, applied to the veterinary field. [ABSTRACT FROM AUTHOR]

Published
2024
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14. AGR - 2 Colloid Cyst: Underrecognized Neuropsychological Sequalae of a Neurosurgical Diagnosis

Author

Bender, Heidi, primary, Bander, Evan, additional, Gavarrete-Olvera, Alice, additional, Giambrone, Julia, additional, Katsman, Kseniya, additional, Muhareb, Samer, additional, Rosen, Kate, additional, Sacks-Zimmerman, Amanda, additional, Seger, Madison, additional, Souweudane, Mark, additional, Tosi, Umberto, additional, and Yap, David L, additional

Published
2023
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15. Endoscopic endonasal repair of encephaloceles of the lateral sphenoid sinus: multiinstitution confirmation of a new classification

Author

Tosi, Umberto, primary, Jackson, Christina, additional, D’Souza, Glen, additional, Rabinowitz, Mindy, additional, Farrell, Christopher, additional, Parsel, Sean M., additional, Anand, Vijay K., additional, Kacker, Ashutosh, additional, Tabaee, Abtin, additional, Zenonos, Georgios A., additional, Snyderman, Carl H., additional, Wang, Eric W., additional, Evans, James, additional, Rosen, Marc, additional, Nyquist, Gurston, additional, Gardner, Paul A., additional, and Schwartz, Theodore H., additional

Published
2023
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19. Lateral Transorbital Endoscope-Assisted Approach to the Cavernous Sinus.

Author

Bander, Evan D., Carnevale, Joseph A., Tosi, Umberto, Godfrey, Kyle J, and Schwartz, Theodore H

Subjects
CAVERNOUS sinus, DERMOID cysts
Abstract

This article, titled "Lateral Transorbital Endoscope-Assisted Approach to the Cavernous Sinus," discusses a surgical technique called the lateral transorbital approach (LTOA) that provides direct access to the cavernous sinus (CS), a challenging area for neurosurgeons due to its small size and delicate structures. The study conducted a retrospective review of six patients who underwent LTOA for various pathologies, including dermoid cyst, schwannoma, prolactinoma, craniopharyngioma, and solitary fibrous tumor. The surgical goals were successfully achieved in all cases, with a mean extent of resection of 64.6%. Half of the patients with preoperative cranial neuropathies showed improvement postoperatively, and there were no new permanent cranial neuropathies. The study concludes that careful case selection and reasonable surgical goals are crucial for a successful outcome using the LTOA technique. [Extracted from the article]

Published
2024
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21. Supplementary Video 1 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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22. Data from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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23. Supplementary Video 4 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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24. Supplementary Video S2 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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25. Supplementary Video 7 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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26. Supplementary Video 6 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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27. Supplementary Video 3 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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28. Supplementary Video 5 from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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29. Supplementary Data from A Murine Model for Quantitative, Real-Time Evaluation of Convection-Enhanced Delivery (RT-CED) Using an 18[F]-Positron Emitting, Fluorescent Derivative of Dasatinib

Author

Wang, Melinda, primary, Kommidi, Harikrishna, primary, Tosi, Umberto, primary, Guo, Hua, primary, Zhou, Zhiping, primary, Schweitzer, Melanie E., primary, Wu, Linda Y., primary, Singh, Ranjodh, primary, Hou, Shengqi, primary, Law, Benedict, primary, Ting, Richard, primary, and Souweidane, Mark M., primary

Published
2023
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30. Endoscopic odontoidectomy for brainstem compression in association with posterior fossa decompression and occipitocervical fusion

Author

Tosi, Umberto, primary, Giantini-Larsen, Alexandra, additional, Mathios, Dimitrios, additional, Kacker, Ashutosh, additional, Anand, Vijay K., additional, Ferdowssian, Kiarash, additional, Baaj, Ali, additional, Härtl, Roger, additional, Rapoport, Benjamin I., additional, Greenfield, Jeffrey P., additional, and Schwartz, Theodore H., additional

Published
2023
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32. Combined Surgical Repair and Venous Sinus Stenting for Patients with Skull Base Encephaloceles and Idiopathic Intracranial Hypertension

Author

Tosi, Umberto, additional, Ramos, Alexander, additional, Rampichini, Margherita, additional, Alexiades, George, additional, Boddu, Srikanth, additional, Cisse, Babacar, additional, Kacker, Ashutosh, additional, Patsalides, Athos, additional, Tabaee, Abtin, additional, Schwarz, Justin, additional, Schwartz, Theodore, additional, and Ramakrishna, Rohan, additional

Published
2023
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42. Utility of Multimodality Molecular Profiling for Pediatric Patients with Central Nervous System Tumors

Author

Rajappa, Prajwal, primary, Eng, Kenneth W, additional, Bareja, Rohan, additional, Bander, Evan D, additional, Yuan, Melissa, additional, Dua, Alisha, additional, Maachani, Uday Bhanu, additional, Snuderl, Matija, additional, Pan, Heng, additional, Zhang, Tuo, additional, Tosi, Umberto, additional, Ivasyk, Iryna, additional, Souweidane, Mark M, additional, Elemento, Olivier, additional, Sboner, Andreas, additional, Greenfield, Jeffrey P, additional, and Pisapia, David J, additional

Published
2022
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43. OVINE ADIPOSE-DERIVED STEM CELLS AS POTENTIAL STEM CELL SOURCE FOR TENDON REPAIR

Author

Haidar Montes, Arlette A., Prencipe, Giuseppe, EL KHATIB, Mohammad, Mauro, Annunziata, Berardinelli, Paolo, Tosi, Umberto, Guy, Wouters, Johannes, Stöckl, Russo, Valentina, and Barboni, Barbara

Subjects
Regenerative medicine, ADSCs, tendon healing, ADSCs, Regenerative medicine, tendon healing
Published
2022

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