Search

The emergence of various diseases such as cardiovascular, aging and cancer are due to oxidative stress as well as the shortage of enough antioxidant materials. Our study was intended to analyze the anticancer, antimicrobial, wound healing as well as hepatoprotective activities of purified sulfated polysaccharides derived from P. pavonica. The sulfated polysaccharide was subjected to partial purification using DEAE cellulose. The sulfated fraction was identified by HPLC, FTIR, 1H-NMR and GC–MS. The results showed that the presence of fucopyranose in sulfated polysaccharide was attached to the O-acetyl groups confirmed by 1H-NMR analysis. Further P. pavonica was carried out for the antioxidant profiling of sulfated polysaccharide through various standard assays. Bioactive principles of sulfated polysaccharides in medicinal fields were confirmed by anticancer activity on MDA-MB-231 breast cancer cells and wound healing activity in four groups of albino rats. The hepatoprotective activity of sulfated fraction was determined based on their biochemical parameters. Emulsification activity of sulfated polysaccharide was also evaluated. Also, it has antimicrobial properties. Our report has pointed out that the supplementation of sulfated polysaccharides would prevent the formation of cancer and oxidative damage of biomolecules. [ABSTRACT FROM AUTHOR]

Determining the total antioxidant capacity (TAC) of biological samples is a valuable approach to measuring health status under oxidative stress conditions, such as infertility and type 2 diabetes. The Trolox equivalent antioxidant capacity (TEAC) assay is the most common approach to evaluating TAC in biological matrices. This assay is typically performed in clinical settings on a microtiter plate using a plate reader. However, the instrumentation and expertise requirements, and the resulting delay in the reporting of assay outcomes, make solution-based TEAC assays impractical for point-of-care or at-home testing, where individuals may want to monitor their health status during treatment. This work introduces the first microfluidic paper-based analytical device (µPAD) that measures TAC in human serum using TEAC assay chemistry. TAC was determined through a colorimetric image analysis of the degree of decolorization of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cations (ABTS●+) by serum antioxidants. The µPAD showed a linear response to Trolox, ranging from 0.44 to 2.4 mM, (r  =  0.999). The performance of paper-based TEAC assays was validated through direct comparison to solution-based TEAC assays. There was a 0.04 mM difference in TAC values between the two platforms, well within one standard deviation of a standard solution-based assay conducted on an aliquot of the same serum sample (±0.25 mM). The µPAD had a limit of detection (LOD) of 0.20 mM, well below the TAC of normal human serum. The results suggest that the proposed device can be used for biological TAC determination and expands the field of TAC analysis in point-of-care health monitoring. [ABSTRACT FROM AUTHOR]

A novel Fe-MoOx nanozyme, engineered with enhanced peroxidase (POD)-like activity through strategic doping and the creation of oxygen vacancies, is introduced to catalyze the oxidation of TMB with high efficiency. Furthermore, Fe-MoOx is responsive to single electron transfer (SET) and hydrogen atom transfer (HAT) mechanisms related to antioxidants and can serve as a desirable nanozyme for total antioxidant capacity (TAC) determination. The TAC colorimetric platform can reach a low LOD of 0.512 μM in solution and 24.316 μM in the smartphone-mediated RGB hydrogel (AA as the standard). As proof of concept, the practical application in real samples was explored. The work paves a promising avenue to design diverse nanozymes for visual on-site inspection of food quality. [ABSTRACT FROM AUTHOR]

Determining the total antioxidant capacity (TAC) of biological samples is a valuable approach to measuring health status under oxidative stress conditions, such as infertility and type 2 diabetes. The Trolox equivalent antioxidant capacity (TEAC) assay is the most common approach to evaluating TAC in biological matrices. This assay is typically performed in clinical settings on a microtiter plate using a plate reader. However, the instrumentation and expertise requirements, and the resulting delay in the reporting of assay outcomes, make solution-based TEAC assays impractical for point-of-care or at-home testing, where individuals may want to monitor their health status during treatment. This work introduces the first microfluidic paper-based analytical device (µPAD) that measures TAC in human serum using TEAC assay chemistry. TAC was determined through a colorimetric image analysis of the degree of decolorization of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cations (ABTS●+) by serum antioxidants. The µPAD showed a linear response to Trolox, ranging from 0.44 to 2.4 mM, (r = 0.999). The performance of paper-based TEAC assays was validated through direct comparison to solution-based TEAC assays. There was a 0.04 mM difference in TAC values between the two platforms, well within one standard deviation of a standard solution-based assay conducted on an aliquot of the same serum sample (±0.25 mM). The µPAD had a limit of detection (LOD) of 0.20 mM, well below the TAC of normal human serum. The results suggest that the proposed device can be used for biological TAC determination and expands the field of TAC analysis in point-of-care health monitoring.

The purpose of this study was to assess the effects of nitrogen (N) fertilization and cultivar on vitamin C content, total antioxidant capacity (TAC), and catalase (CAT) activity in amaranth leaves, as well as in the rhizosphere and non-rhizosphere soil. For this purpose, a 3-year split-plot field experiment was conducted, which included the following factors: N fertilization (kg · ha-1: N0 - control, N1 - 60, N2 - 90, N3 - 120, N4 - 150), amaranth cultivars ('Rawa' and 'Aztek') and developmental stages (BBCH 13, BBCH 16, BBCH 19). The factor that most significantly differentiated the vitamin C content in the leaves was the cultivar, followed by the development stage, N fertilization, and weather conditions. The cultivar 'Aztek', stage BBCH 13, N3 fertilization, and weather conditions during the last year of the experiment gave the best results in this regard. CAT activity in the leaves significantly depended on N fertilization and developmental stage. It increased with the higher N dose and decreased with plant development. The TAC of the leaves depended only on the developmental stage and weather conditions. The highest TAC was observed at BBCH 13 and the third year of the study. CAT in the rhizosphere significantly depended on N fertilization, cultivar, and developmental stage, while in the non-rhizosphere zone, it depended on N fertilization, developmental stage, and weather conditions. This study is an essential addition to the knowledge on the use of amaranth seed forms as a vegetable with high nutritional value and antioxidant properties, as well as the effect of this plant on soil biological properties. [ABSTRACT FROM AUTHOR]

The purpose of this study was to assess the effects of nitrogen (N) fertilization and cultivar on vitamin C content, total antioxidant capacity (TAC), and catalase (CAT) activity in amaranth leaves, as well as in the rhizosphere and non-rhizosphere soil. For this purpose, a 3-year split-plot field experiment was conducted, which included the following factors: N fertilization (kg ∙ ha–1: N0 – control, N1 – 60, N2 – 90, N3 – 120, N4 – 150), amaranth cultivars (‘Rawa’ and ‘Aztek’) and developmental stages (BBCH 13, BBCH 16, BBCH 19). The factor that most significantly differentiated the vitamin C content in the leaves was the cultivar, followed by the development stage, N fertilization, and weather conditions. The cultivar ‘Aztek’, stage BBCH 13, N3 fertilization, and weather conditions during the last year of the experiment gave the best results in this regard. CAT activity in the leaves significantly depended on N fertilization and developmental stage. It increased with the higher N dose and decreased with plant development. The TAC of the leaves depended only on the developmental stage and weather conditions. The highest TAC was observed at BBCH 13 and the third year of the study. CAT in the rhizosphere significantly depended on N fertilization, cultivar, and developmental stage, while in the non-rhizosphere zone, it depended on N fertilization, developmental stage, and weather conditions. This study is an essential addition to the knowledge on the use of amaranth seed forms as a vegetable with high nutritional value and antioxidant properties, as well as the effect of this plant on soil biological properties.

This article explores the correlation between salivary biomarkers, such as glutathione peroxidase (GPX), total antioxidant capacity (TAC), and superoxide dismutase (SOD), and their association with oral health for children in competitive sports. Saliva has emerged as a valuable resource for evaluating physiological and pathological conditions due to its non-invasive collection method and easy storage. This study examines the potential of GPX, TAC, and SOD as salivary biomarkers for assessing the impact of competitive sports on children's oral health. It discusses the potential implications of increased oxidative stress due to intense physical activity and the role of antioxidant defense mechanisms in maintaining oral health. In total, 173 children aged between 6 and 17 were divided into three groups, 58 hockey players, 55 football players, and 60 in the control group, and examined to assess their oral hygiene and dental and periodontal health. Saliva was collected, centrifuged, and the supernatant was analyzed for the relevant biomarkers. The findings seem to suggest that salivary biomarkers, like GPX, TAC, and SOD, might serve as indicators of the physiological response to competitive sports in children, as well as indicators of oral health, especially dental cavities, and periodontal disease. Statistical analysis showed significant differences between the groups, with better values for athletes, regardless of age, sex, or activity type. Understanding the relationship between salivary biomarkers and competitive sports in children can have significant implications for monitoring and optimizing the health and performance of young athletes. Further research is needed to establish the specific associations between these biomarkers and the effects of several types and intensities of sports activities on oral health in children. [ABSTRACT FROM AUTHOR]

Total antioxidant capacity (TAC) is currently considered as a vital indicator of food quality in antioxidant ability and attracts much attention for human healthcare. It is thus of great significance to realize the accurate and rapid detection of TAC in foods. Herein, we have constructed a preferable hybrid nanozyme based on the mesoporous silica-stabilized CuO composited Fe3O4 nanoparticles (Fe3O4@MSNs@CuO, FMC NPs), which possess the enhanced peroxidase (POD)-like activity via cascade response for specific and sensitive determination of TAC in fruit foods. The results showed the hybrid nanozyme displayed a remarkable POD-like activity, excellent selectivity and sensitivity, and the limit of detection (LOD) of the colorimetric sensor was 6.13 mM with the concentration range from 10 to 45 mM. Therefore, the fabricated hybrid nanozyme can be regarded as an effective biosensor for the evaluation of antioxidant quality in fruit foods in future. Key points: • The stabilized bimetallic nanozyme was constructed for TAC analysis in fruits. • The hybrid nanozyme possessed the enhanced POD-like activity by cascading effects. • The nanozyme was an effective biosensor for antioxidant quality evaluation in fruits. [ABSTRACT FROM AUTHOR]

In this study, the Digital Image-Based Colorimetric Detection Method developed by Bakırdere et al. was used to find the TAC (Total Antioxidant Capacity) value of tea samples from different brands. To determine the total amount of antioxidants in tea samples, the CUPRAC (cupric ion reducing antioxidant capacity) method, which is widely used in antioxidant determination, was combined with a digital image-based colorimetric detection system. To use in our study, a box with opaque wood material measuring 24 cm x 19 cm x 17 cm (width/length/depth) was designed and manufactured. In the analysis, the oxidation reaction between the chromogenic copper(II)-neocuproine (Cu(II)-Nc) reagent and antioxidants was utilized. The color change that occurs as a result of the oxidation was calculated using an application on smartphones. In our study, analyzes were performed on 4 different brand tea extract samples (tea A, tea B, tea C, tea D) to determine the total antioxidant capacity of ascorbic acid equivalent. The TAC values for ascorbic acid equivalent in tea extract samples were found as 380 ± 8 mg/L (tea A), 402 ± 4 mg/L (tea B), 213 ± 3 mg/L (tea C), 232 ± 4 mg/L (tea D) using the digital image-based colorimetric detection systems.

Background: The relationship between dietary antioxidants and breast cancer (BrCa) has been investigated in various studies. As a limitation, they generally investigate the relationship between one specific antioxidant and the risk/odds of BrCa, and synergistic or inhibitory effects are less considered. Dietary antioxidant index (DAI) is a reliable nutritional tool that evaluates total nutritional antioxidant capacity and is validated with serum total antioxidant capacity (TAC) and Malondialdehyde (MDA). Our study aimed to investigate the association between the DAI and the odds of BrCa and the correlation between the DAI and pathobiological markers. We hypnotized a correlation between DAI and pathobiological markers, and there is an association between DAI and the odds of BrCa. Methods: Our study included 145 incidence cases of BrCa and 148 hospital-based controls. Controls were randomly selected from patients attending the same center and were frequency-matched on age (±10 years). DAI was calculated based on a valid semi-quantitative 168-item food frequency questionnaire data. DAI standardizes intake of major dietary antioxidants, including vitamins A, E, C, selenium, zinc, and magnesium, and presents them as summarized scores. Results: Modeling with multivariable regressions adjusting for major confounders including age, education, body mass index, occupation, alcohol, smoking, pregnancy, history of cancer, menarche age, metabolic equivalent of task, hormone replacement therapy, and total energy intake, there was a significant association between odds of BrCa and DAI (odds ratio = 0.18; 95% confidence interval:0.09-0.37; p-value= <0.01). After multiple controls, there was a significant and weak reverse correlation between DAI and the number of lymph node(s) (correlation coefficient= −.140; p-value = 0.05). Conclusion: Our study supports the hypothesis that dietary antioxidants intake is associated with a reduced odds of BrCa. Evidently, DAI can reveal these relationships better than a single study of antioxidants. However, further studies are needed to confirm or refute these results. [ABSTRACT FROM AUTHOR]

This study was undertaken to investigate the photoperiod- and age-related variability between the activity of oxidative stress biomarkers in rats with different physiological reactivity estimated by different resistance to hypoxia. The study was carried out on 96 male Wistar rats divided into 16 groups based on resistance to hypoxia (LR, low resistance, HR, high resistance) and age, i.e. 6 and 21 months. The studies were conducted at four photoperiod points: winter (January), spring (March), summer (July), and autumn (October). Lower levels of oxidative stress biomarkers (P

Numerous antioxidants with similar physicochemical properties make them very difficult to be discriminated because of the similarly analytical signal output. To solve this obstacle, a novel wavelength-dependent and three-channel photoelectrochemical (PEC) sensor array based on Bi 2 WO 6 /Vo-TiO 2 electrospun nanostructures was established for the identification of antioxidants in natural teas via linear discriminant analysis (LDA). [Display omitted] • A novel wavelength-dependent and three-channel PEC sensor array was established. • Heterogeneous interfaces and oxygen vacancies enhanced the PEC performance. • LDA-assisted PEC sensor array was beneficial for achieving multiplex assay. • PEC sensor array was able to efficiently estimate TAC in actual tea samples. Evaluating total antioxidant capacity (TAC) have been playing a vital role in health guidelines, the analysis of nutritional composition in foods, and the diagnosis and treatment of oxidative stress-induced disease. However, numerous antioxidants with similar physicochemical properties make them very difficult to be discriminated because of the similarly analytical signal output. In this work, a novel wavelength-dependent and three-channel photoelectrochemical (PEC) sensor array based on Bi 2 WO 6 /Vo-TiO 2 electrospun nanostructures was established for the identification of antioxidants in natural teas. The formation of heterojunction interfaces between Bi 2 WO 6 nanosheet and Vo-TiO 2 NFs, and the introduction of the oxygen vacancies in TiO 2 NFs promoted the visible light absorption ability and electron transfer rate of Bi 2 WO 6 /Vo-TiO 2 NFs, causing the differences of photocurrent response to different antioxidants under different wavelengths. Furthermore, excited wavelengths, antioxidant types, and photocurrent were regarded as three-factors of fingerprint to accurately discriminate five antioxidants via linear discriminant analysis (LDA). Finally, the PEC sensor array was able to estimate TAC in five actual tea samples (green, yellow, black, white, and dark teas). This development of LDA-assisted multiplex PEC fingerprinting sensor array showed a good promise for the identification and authentication of agricultural products, and the establishment of the database. [ABSTRACT FROM AUTHOR]

Background: The pathogenesis of diabetic nephropathy (DN) involves multiple factors, and the exact mechanisms remain uncertain. Thus, the aim of the current study is to assess the oxidant/antioxidant status among children and adolescents with type 1 diabetes(T1D) and diabetic nephropathy (DN). Methods: A comparative cross-sectional study including 100 patients with T1D; equally divided into 2 groups according to urinary albumin excretion rate (UAER). Both groups of T1D were compared with age and gender matched healthy controls. To assess oxidant/antioxidant status, malondialdehyde (MDA) and total antioxidant capacity (TAC) were assessed using colorimetric method. Results: The MDA levels were significantly higher in patients with DN (9.24 nmol/ml) compared with patients with normoalbuminuria (1.99 nmol/ml) and healthy controls (0.78 nmol/ml) (P < 0.001). Unlike MDA, TAC levels were significantly lower in patients with T1D compared to healthy subjects. Among participants with T1D, TAC levels were significantly higher among patients with DN (P < 0.001). A significant negative correlation was found between MDA and TAC levels (r=-0.655, P < 0.01). Furthermore, a significant positive correlation was elicited between MDA and both HbA1C (r = 0.5, P < 0.001) and UAER (r = 0.7, P < 0.001). Conclusion: T1D is associated with heightened levels of oxidative stress compared to those without the condition, with higher level of oxidant/antioxidant imbalance in DN. This suggests that oxidative stress could potentially serve as a significant factor in the development DN. [ABSTRACT FROM AUTHOR]

In this study, the Digital Image-Based Colorimetric Detection Method developed by Bakırdere et al. was used to find the TAC (Total Antioxidant Capacity) value of tea samples from different brands. To determine the total amount of antioxidants in tea samples, the CUPRAC (cupric ion reducing antioxidant capacity) method, which is widely used in antioxidant determination, was combined with a digital image-based colorimetric detection system. To use in our study, a box with opaque wood material measuring 24 cm x 19 cm x 17 cm (width/length/depth) was designed and manufactured. In the analysis, the oxidation reaction between the chromogenic copper(II)-neocuproine (Cu(II)-Nc) reagent and antioxidants was utilized. The color change that occurs as a result of the oxidation was calculated using an application on smartphones. In our study, analyzes were performed on 4 different brand tea extract samples (tea A, tea B, tea C, tea D) to determine the total antioxidant capacity of ascorbic acid equivalent. The TAC values for ascorbic acid equivalent in tea extract samples were found as 380 ± 8 mg/L (tea A), 402 ± 4 mg/L (tea B), 213 ± 3 mg/L (tea C), 232 ± 4 mg/L (tea D) using the digital image-based colorimetric detection systems. [ABSTRACT FROM AUTHOR]

Although coffee has been reported as a major contributor to antioxidants in the diet, there are limited studies assessing how brewing methods and types of coffee affect antioxidant capacity of coffee consumers. This study was aimed to investigate the impact of coffee preparation on total phenolic content in brewed coffee extracts and their contribution to antioxidant status. We examined how the different brewing methods affect total phenolic content and antioxidant capacity in brewed coffee extracts, then assessed the antioxidant status of coffee consumers after drinking coffee. Results showed that consuming capsule versus French press coffee provides a higher total phenolic content and drinking capsule coffee leads to greater antioxidant status as shown by the higher total antioxidant capacity in urine. Further studies can expand on this by using a broader range of brewing methods to evaluate the contribution of brewing methods of coffee to antioxidant status. [ABSTRACT FROM AUTHOR]

The aim of this work was to determine advanced the oxidative protein products (AOPPs), total antioxidant capacity (TAC), and myeloperoxidase activity (MPO) in the saliva of patients undergoing orthodontic treatment with clear removable aligners in comparison with another group in treatment with fixed passive self-ligating brackets applying light forces, before treatment, after 30 days, and after 90 days of treatment. This non-randomized clinical trial recruited patients consecutively, all of which were over 18 years of age and due to undergo orthodontic treatment. They were divided into two groups according to treatment type: Group A, 48 patients treated with clear aligners (Invisalign®); and Group B, 19 patients treated with Damon System® 0.22″ self-ligating brackets applying light forces. Saliva samples were collected by a single clinician following the same protocol and underwent three analyses—AOPPs, TAC, and MPO levels–at baseline before placing the apparatus, after 30 days, and after 90 days treatment. Orthodontic treatment, whether with clear aligners or fixed self-ligating brackets and light forces, increased AOPPs after the first 30 days of treatment. During the initial phases of orthodontic treatment, neither clear aligners nor fixed self-ligating brackets applying light forces showed changes in TAC and MPO. Orthodontic treatment with both clear aligners and fixed apparatus self-ligating brackets applying light forces increases oxidative stress (AOPPs) after the first 30 days of treatment. There are no differences in AOPP levels between treatment with clear aligners and self-ligating brackets during the first 90 days of treatment. The antioxidative capacity of saliva during the initial phases of orthodontic treatment, whether with self-ligating brackets or clear aligners, does not undergo significant changes. With either orthodontic technique, the patients' salivary antioxidant capacity is similar. [ABSTRACT FROM AUTHOR]

[Display omitted] This study aimed to assess the effects of Parascaris equorum (P. equorum) in infected donkeys through evaluation the oxidative stress and different gene parameters in infected tissues. Fifty donkeys were examined in Giza Zoo abattoir from the period of January to March 2021. Blood and sera samples were collected from each examined donkey. P. equorum were subjected for identification through scanning electron microscope study and the infected tissues were subjected into gene expression analysis using two genes; interleukin 1β (IL1- β); and pro-inflammatory cytokines (TNF-α) with assessment of the antioxidant and free radicals released from the animals during the infection. Eighteen donkeys were positive for P. equorum adult or larvae by postmortem examination of the intestine and abdomen with prevalence rate of 36 %. The examined infected donkeys with P. equorum showed significantly higher of Total antioxidant capacity (TAC) levels and the serum malondialdehyde (MDA) 2.45 ± 0.53 than that in non-infected control donkeys. The levels of AST enzyme were 278.54 ± 0.45 while ALT enzyme was 14.97 ± 0.87 which was significantly higher than that of control negative donkeys. The infected donkeys with P. equorum showed significantly upregulation of the TNF-α and IL-1β which classify according to number of collected worms. The P. equorum infected donkeys exerted at least 100 eggs of parasite in feces. The fecal egg count was marked decreased after treatment with moxidectin. Moxidectin is considered a novel active ingredient that has a marvelous result with high persistency and protection for long time, in addition to, broad spectrum activity and low or no resistance. We recommend the periodical deworming with different molecules as more economic and lifesaving over a single treatment every 12 months parallel with parasitic testing. [ABSTRACT FROM AUTHOR]

Featured Application: The lower or less toxic effects of CNSs in an in vivo genetic model such as Drosophila melanogaster support its possible use in biological applications such as drug carriers and others. Toxicological profiling of the novel carbon materials has become imperative, owing to their wide applicability and potential health risks on exposure. In the current study, the toxicity of mesoporous carbon nanospheres synthesized from waste onion peel was investigated using the genetic animal model Drosophila melanogaster. The survival assays at different doses of carbon nanoparticles suggested their non-toxic effect for exposure for 25 days. Developmental and behavioral defects were not observed. The biochemical and metabolic parameters, such as total antioxidant capacity (TAC), protein level, triglyceride level, and glucose, were not significantly altered. The neurological toxicity as analyzed using acetylcholinesterase activity was also not altered significantly. Survival, behavior, and biochemical assays suggested that oral feeding of mesoporous carbon nanoparticles for 25 days did not elicit any significant toxicity effect in Drosophila melanogaster. Thus, mesoporous carbon nanoparticles synthesized from waste onion peel can be used as beneficial drug carriers in different disease models. [ABSTRACT FROM AUTHOR]

BACKGROUND: Oxidative stress and chronic inflammation are among the leading causes of coronary heart disease (CHD). Studies investigated the relationship between dietary antioxidants and the risk/odds of CHD, and contradictory results have been reported. Dietary antioxidant index (DAI) is a novel and reliable nutritional tool that examines the diet's overall antioxidant capacity. Its validity was examined using serum total antioxidant capacity and malondialdehyde. OBJECTIVE: This study aimed to investigate the relationship between DAI score and odds of CHD. METHODS: In this incidence case-control study, 320 individuals with a definitive diagnosis of CHD and 320 participants without CHD or related risk factors attending the same hospitals/polyclinics were selected as the case and control groups. We estimated the DAI by summing up six standardized intakes of major dietary antioxidants, including manganese, vitamin E, A, C, selenium, and zinc. RESULTS: Modeling DAI categorized according to the median (–0.38), in multi-adjusted model showed a significant protective association with the odd of CHD (OR = 0.72; 95%CI:0.51–0.99, p-value = 0.05). Also, modeling DAI as a continuous variable in multi-adjusted models (OR = 0.94;95%CI:0.90–0.95; p-value = 0.01) showed significant results. CONCLUSION: Using the DAI to investigate the relationship between dietary antioxidants and CHD can show more realistic results than a single study of antioxidants. [ABSTRACT FROM AUTHOR]

Abstract Background Gastric cancer (GC) incidence and mortality are rapidly growing worldwide. It is estimated that more than 1,000,000 new cases are diagnosed each year, and more than 78,000 people lose their lives due to GC. The association between dietary antioxidants and GC has been shown in some studies. However, because of the discrepancy between the findings and the lack of a valid indicator, it seems necessary to design and validate the Dietary Antioxidant Index (DAI) to examine the diet's total antioxidant content. The present study aimed to survey the validity of DAI and its association with the odds of GC. Methods In this hospital-based case–control study, 82 patients with GC and 95 healthy controls were examined. We used a 168-item food frequency questioner to assess dietary intakes. The DAI was calculated based on the intake of vitamin A, C, E, and selenium, manganese, and zinc. We standardized each of the six vitamins and minerals by subtracting the global mean and dividing by the global standard deviation to calculate DAI. We then calculated the DAI by summing up the standardized intakes of these vitamins and minerals of the individuals with equal weight. Results We observed a significant correlation between DAI and total antioxidant capacity (TAC) after controlling for age, body mass index (BMI), energy intake, smoking and fasting blood sugar, education, total fat intake, helicobacter pylori infection, total cholesterol, and saturated fatty acid (SFA) intakes. Results obtained from modeling DAI as a continuous variable in relation to GC showed a negative association after adjustment for age and in the multivariable analysis (OR = 0.64, CI = 0.43–0.95). Conclusion DAI is a valid indicator of dietary antioxidants assessments, and it can be used as a predictor of antioxidant status due to its correlation with serum antioxidant levels. The results showed that dietary antioxidants have a significant relationship with GC, which indicates the importance of antioxidants in this cancer's etiology.

The present experiment was carried out with the objective to investigate the antioxidant status of peripartumMurrah buffaloes by assessing total antioxidant capacity (TAC), neutrophil profile, in vitro phagocytic activity,myeloperoxidase (MPO) activity, IL-8. Based on uterine fluid scoring, white side test buffaloes (n=24) were classifiedinto healthy (n=11) and uterine infected buffaloes (n=13). Blood samples were collected 7 day before calving(day 7), on day of calving (day 0) and after calving (day 7, 14, 21 and 35) for estimation of the mentioned parametersand uterine fluid samples were collected during postpartum period, i.e. day 7, 14, 21, 35 after calving for groupingof buffaloes. Results showed that TAC concentration decreased on day of calving and elevated afterwards whereasthe percentage of neutrophils was significantly higher on 14th, 21th and 35th day after calving in uterine infectedbuffaloes compared to healthy group. Phagocytic activity was lower during peripartum period and IL-8 concentrationwas significantly higher on 21 day after calving in uterine infected buffaloes as compared to healthy buffaloes.Pearson correlation between serum TAC with uterine score gave a non-significant correlation of –0.33. From theabove research it can be concluded that lower concentration of TAC, decreased phagocytic activity of neutrophilsin uterine infected buffaloes indicate poor anti-oxidant status to combat against oxidative stress making animalssusceptible to infections. Therefore, proper nutritional management with additives should be provided duringperipartum period to reduce the incidence of uterine infections as well as for better welfare.

Background: The application of herbicides has been a common practice in worldwide agriculture, mainly with the goal to increase efficacy of weed control. So, most farmers faced a greater risk of herbicide exposure to develop adverse health effects from oxidative stress-induced herbicides. The aim of this study is to determine the urinary total antioxidant capacity (TAC) and 8-isoprostane levels in farmers using the mixture of herbicides in Long District, Phrae Province, Thailand. Methods: Ninety-three participants were recruited. The spot urine samples (pre- and post-work) were collected. The urinary TAC was determined using ABTS radical scavenging assay. The urinary 8-isoprostane level was investigated by commercial ELISA kit. Results: Most farmers worked on a farm during 1-5 h/day. Fifty-five percents of participants used the mixture of herbicide while working between glyphosate and paraquat as well as glyphosate and 2,4-D. The urinary TAC levels in pre-work urine sample of farmers applied combining herbicides were significantly higher than that level in their post-work urine sample. However, there was no significant difference between pre- and post-work urinary 8-isoprostane in farmers applied combining herbicides. Conclusion: The results suggest that occupational exposure to mixture of glyphosate herbicides plus paraquat or 2,4-D could induce abnormal oxidative stress value especially antioxidant defense among agricultural workers. [ABSTRACT FROM AUTHOR]

Coronavirus disease 2019 (COVID-19), as a dangerous global pandemic, has led to high morbidity and mortality in all countries. There is a lot of evidence for the possible role of oxidative stress in COVID-19. In the present study, we aimed to measure the levels of glutathione (GSH), total antioxidant capacity (TAC) and total oxidant status (TOS) in the serum of patients with COVID-19.A total of 96 individuals with and without COVID-19 were enrolled and divided into four groups, including hospitalised group in non–intensive care units (non-ICU) (n = 35), hospitalised group in intensive care units with endotracheal intubation (EI) (ICU with EI) (n = 19), hospitalised group in intensive care units without endotracheal intubation (ICU without EI) (n = 24) and healthy people without COVID-19 disease as our control group (n = 18). The present study revealed that the TOS level was significantly lower in the group of control (p = 0.001), and level of GSH remarkably increased in the patients' groups (p

Abstract Corneal endothelial decompensation is a serious condition that frequently requires treatment via corneal transplantation which contributes to a global shortage in donor corneas. Therefore, the purpose of this study was to analyze the influence of aqueous humor total antioxidant capacity (TAC) on corneal endothelial health. There is an urgent need for discovering protective factors to combat corneal endothelial cell (CEC) loss. For methods, we developed a cupric ion‐based TAC (CuTAC) assay to analyze TAC level in a small volume of aqueous humor, that is, 10 μL per test, and examined the influences of ascorbic acid (AA) and antioxidant proteins on aqueous humor TAC. To broaden the investigation, we conducted a case–control study with patients classified into two groups, an insufficient endothelial cell density (ECD

Corneal endothelial decompensation is a serious condition that frequently requires treatment via corneal transplantation which contributes to a global shortage in donor corneas. Therefore, the purpose of this study was to analyze the influence of aqueous humor total antioxidant capacity (TAC) on corneal endothelial health. There is an urgent need for discovering protective factors to combat corneal endothelial cell (CEC) loss. For methods, we developed a cupric ion‐based TAC (CuTAC) assay to analyze TAC level in a small volume of aqueous humor, that is, 10 μL per test, and examined the influences of ascorbic acid (AA) and antioxidant proteins on aqueous humor TAC. To broaden the investigation, we conducted a case–control study with patients classified into two groups, an insufficient endothelial cell density (ECD < 2100 cells/mm2) group, and a control group. These groups were formed based on baseline ECD values and were used to evaluate the influence of aqueous humor TAC and AA on overall corneal endothelial health. A CuTAC assay was used to accurately measure aqueous humor TAC without the need for sample dilution. After analyzing a total of 164 human aqueous humor samples, we found that AA was the major contributor to aqueous humor TAC (73.2%). In addition, TAC and AA levels in the IECD and control groups were both found to be significantly different (1.168 vs. 1.592 mM, p = 0.009 and 0.856 vs. 1.178 mM, p = 0.016). TAC and AA were considered independent protective factors against IECD with adjusted odds ratios of 0.02 (p = 0.017) and 0.023 (p = 0.033), respectively. In conclusion, aqueous humor TAC and AA contribute to the maintenance of sufficient corneal ECD, and our CuTAC assay can be a useful tool for analyzing TAC using only a small aqueous humor sample volume. [ABSTRACT FROM AUTHOR]

The intention of the study was evaluated for purification and characterization of exopolysaccharides from Lactobacillus paracasei ; was isolated from homemade Sauerkraut sample collected from Sivakasi, Tamil Nadu, India, confirmed by biochemical and gene sequencing (16S rRNA). The purification and characterization of exopolysaccharides from candidate bacterium were studied on appearance, solubility of the EPS, carbohydrate estimation, emulsifying activity, sulphate, protein, uronic acid content, FTIR, HPLC and GC-MS analysis. The percentage of elemental carbon, (54.36%) hydrogen (21.74%), nitrogen (9.63%) and sulphur content (18.03%) were recorded in exopolysaccharides. The emulsification index (E24) of EPS was higher in toluene (79.20) and benzene (78.867) supplemented medium. FTIR spectrum of the candidate bacterial EPS confirmed presence of sulphate compounds, carboxyl group, and hydrogen bonded compounds etc. EPS exhibited 76.34% of Total Antioxidant Capacity (TAC), 71.15% of reducing power, 68.65% of Hydrogen Peroxide scavenging activity and also 60.31% DPPH radical scavenging activity. The potential antioxidant properties observed in exopolysaccharides from Lactobacillus paracasei is considered as valuable drugs. [ABSTRACT FROM AUTHOR]

Toxicological profiling of the novel carbon materials has become imperative, owing to their wide applicability and potential health risks on exposure. In the current study, the toxicity of mesoporous carbon nanospheres synthesized from waste onion peel was investigated using the genetic animal model Drosophila melanogaster. The survival assays at different doses of carbon nanoparticles suggested their non-toxic effect for exposure for 25 days. Developmental and behavioral defects were not observed. The biochemical and metabolic parameters, such as total antioxidant capacity (TAC), protein level, triglyceride level, and glucose, were not significantly altered. The neurological toxicity as analyzed using acetylcholinesterase activity was also not altered significantly. Survival, behavior, and biochemical assays suggested that oral feeding of mesoporous carbon nanoparticles for 25 days did not elicit any significant toxicity effect in Drosophila melanogaster. Thus, mesoporous carbon nanoparticles synthesized from waste onion peel can be used as beneficial drug carriers in different disease models.

Background: Diabetes mellitus (DM) is a highly prevalent non-communicable disease in the world. Current investigations evolved that oxidative stress is also a major risk factor to cause type 2 diabetes mellitus due to impairment of antioxidant defense system in various biological fluids. Methodology: In this cross-sectional study, 70 type 2 diabetes mellitus subjects and 30 normal healthy subjects of both genders were selected from various health care centers of Karachi, Pakistan for a study period of six months June 2017 – December 2017. The total antioxidant capacity (TAC) concentration was measured in serum by enzyme-linked immunosorbent assay (ELISA) technique using Caymans Antioxidant Assay. The biochemical parameters and anthropometric measurements were estimated by standardized methods. Data was analyzed using the statistical program Statistical Package for the Social Sciences (SPSS) version 10.0. Results: According to the study results TAC was significantly reduced (**0.05 ± 0.00 mmol /L) in type 2 diabetes mellitus subjects compared to normal healthy subjects (0.13 ± 0.02 mmol /L). It was noted that diastolic blood pressure (DBP), body mass index (BMI), and triglycerides (TG’s) were significantly increased while high density lipoprotein-cholesterol (HDL-C) was significantly reduced in diabetic subjects than the comparative healthy individuals. Conclusion: This study showed that decreased levels of TAC and HDL-C in type 2 DM patients with increased levels of BMI, systolic blood pressure (SBP), fasting blood sugar (FBS), DBP, and total cholesterol (TC) which may cause oxidative stress and increase the progression of cardiovascular disease (CVD) and other metabolic diseases. Modifications in dietary habits and intake of antioxidant foods or supplements may diminish the process of oxidative stress which may consequently decrease CVD and other severe clinical outcomes.

Abstract Background Several studies have shown significant associations between manganese superoxide dismutase (MnSOD) Val16Ala polymorphism and diabetic complications, but this association has not been explored in relation with chronic kidney disease (CKD) in Type 2 diabetes mellitus (T2DM) patients. Total antioxidant capacity (TAC) level changes in diabetic condition and may play important role in onset or progression of the disease and its complications. The present study investigated the association of MnSOD Val16Ala polymorphism and serum TAC with the risk of CKD in T2DM patients. Methods This nested case-control study included 280 type 2 diabetic patients with CKD and 280 age, sex and diabetes duration-matched control subjects selected from the participants of the Tehran Lipid and Glucose Study. MnSOD val16Ala (rs4880) SNP was genotyped by the Tetra-Primer ARMS-polymerase chain reaction analysis. Serum TAC was measured using ferric-reducing antioxidant power assay. Statistical analysis was performed using STATA statistical package v.12.0 or SPSS (Version 22.0). Results The Ala allele of the MnSOD Val16Ala polymorphism was associated with a lower risk of CKD (odds ratio (OR), 0.55; 95% confidence interval (CI), 0.36–0.84; P = 0.006). Median serum TAC in CKD group was 920 μmol/L and was significantly lower (p

Quantitative evaluation of the antioxidant capacity of foods is of great significance for estimating food’s nutritional value and preventing oxidative changes in food. Herein, we demonstrated an easy and selective colorimetric method for the total antioxidant capacity (TAC) assay based on 3,3’,5,5’-tetramethyl-benzidine (TMB), hydrogen peroxide (H2O2) and synthetic Lycium barbarum polysaccharide-iron (III) chelate (LBPIC) with high peroxidase (POD)-like activity. The results of steady-state kinetics study showed that the Km values of LBPIC toward H2O2 and TMB were 5.54 mM and 0.16 mM, respectively. The detection parameters were optimized, and the linear interval and limit of detection (LOD) were determined to be 2–100 μM and 1.51 μM, respectively. Additionally, a subsequent study of the determination of TAC in six commercial fruit and vegetable beverages using the established method was successfully carried out. The results implied an expanded application of polysaccharide-iron (III) chelates with enzymatic activity in food antioxidant analysis and other biosensing fields.

The aim of this study was to define the oxidative-antioxidative capacity and lipid profile in cattle with brucellosis. A total of 32 cattle of Simmental breed were used in the study, among those, 22 demonstrated microbiologically positive for brucellosis while 10 were negative. Biochemical analysis included total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), very low-density lipoprotein-cholesterol (VLDL-C) and low-density lipoprotein-cholesterol (LDL-C). TAC value was found to be statistically significantly lower (P=0.007), and TOC and OSI values were found to be significantly higher [respectively (P=0.013) and (P=0.002)] in cattle with brucellosis compared to the control group. TC (P=0.012) and LDL-C (P=0.0004) values were significantly increased, and TG (P=0.004), HDL-C (P=0.023) and VLDL-C (P=0.004) values were significantly decreased compared to the control group. As a conclusion, it was determined that Brucella infection lead to important changes in the oxidative-antioxidative capacity and lipid profile in cattle. These data may contribute to the diagnosis of the disease and especially to the determination of inflammation severity.

The purpose of this study was to investigate the possible role of PON-1, an antioxidant lipophilic enzyme linked to HDL-C (high-density lipoprotein cholesterol), on the pathophysiology and clinical follow-up of acute pancreatitis. Biochemical tests, PON-1 and oxidative stress parameters (malonyl dialdehyde, MDA; superoxide dismutase, SOD; total antioxidant capacity, TAC) were evaluated in the sera of patients with acute pancreatitis at admission (day 0), day 3 and day 10 of follow-up, between June and September 2017. SPSS 13.0 statistical software package programme was used for statistical analyses.Mean age was 51.4 of the total 25 patients. Ranson scores were 0–1 points (60%), 3–4 points (24%) and 5–6 points (16%). CTSI (computed tomography severity index) scores were calculated, and most of the patients were seen to have mild or average pancreatitis (96%). While total cholesterol, triacylglycerol and LDL-C (low-density lipoprotein) levels stayed in their normal limits, there was a significant decrement tendency. HDL-C level was seen to rise significantly above its upper limit at day 10 (p < 0.001). Mean PON-1 levels were measured as 69.23, 76.72 vs. 113.15 U/mL at days 0, 3 and 10, respectively; and it was positively correlated with HDL-C (p < 0.001). Serum SOD increased also in parallel with PON-1 (20.49 vs. 39.46 U/mL) while MDA level decreased significantly (3.9 vs. 2.28 μM, p < 0.001). TAC was seen to rise significantly after treatment (0.52 vs. 1.22 mM). In conclusion, decreased PON-1 and HDL-C together with antioxidants SOD and TAC at the early period of acute pancreatitis were seen to rise after treatment, while the previously higher MDA level decreased in parallel. This reveals the importance of the balance between oxidative stress and antioxidant defense mechanisms in clinical progression of the disease, and the potential of PON-1 as a promising clinical marker.

The purpose of this study was to investigate the possible role of PON-1, an antioxidant lipophilic enzyme linked to HDL-C (high-density lipoprotein cholesterol), on the pathophysiology and clinical follow-up of acute pancreatitis. Biochemical tests, PON-1 and oxidative stress parameters (malonyl dialdehyde, MDA; superoxide dismutase, SOD; total antioxidant capacity, TAC) were evaluated in the sera of patients with acute pancreatitis at admission (day 0), day 3 and day 10 of follow-up, between June and September 2017. SPSS 13.0 statistical software package programme was used for statistical analyses.Mean age was 51.4 of the total 25 patients. Ranson scores were 0–1 points (60%), 3–4 points (24%) and 5–6 points (16%). CTSI (computed tomography severity index) scores were calculated, and most of the patients were seen to have mild or average pancreatitis (96%). While total cholesterol, triacylglycerol and LDL-C (low-density lipoprotein) levels stayed in their normal limits, there was a significant decrement tendency. HDL-C level was seen to rise significantly above its upper limit at day 10 (p < 0.001). Mean PON-1 levels were measured as 69.23, 76.72 vs. 113.15 U/mL at days 0, 3 and 10, respectively; and it was positively correlated with HDL-C (p < 0.001). Serum SOD increased also in parallel with PON-1 (20.49 vs. 39.46 U/mL) while MDA level decreased significantly (3.9 vs. 2.28 μM, p < 0.001). TAC was seen to rise significantly after treatment (0.52 vs. 1.22 mM). In conclusion, decreased PON-1 and HDL-C together with antioxidants SOD and TAC at the early period of acute pancreatitis were seen to rise after treatment, while the previously higher MDA level decreased in parallel. This reveals the importance of the balance between oxidative stress and antioxidant defense mechanisms in clinical progression of the disease, and the potential of PON-1 as a promising clinical marker. [ABSTRACT FROM AUTHOR]

Objective: Oxidative stress and inflammation play an important role in the pathogenesis of diabetes and its complication. In this study, we aimed to evaluate and compare oxidative stress markers and antioxidant enzymes activity, as well as Interleukin 6 (IL-6) level in newly diagnosed type 2 diabetes mellitus (T2DM) patients and healthy subjects. Material and methods: 30 newly diagnosed type 2 diabetes patients and 30 healthy subjects (age and sex matched) were recruited in this study. After anthropometric parameters measurement, blood sample were collected from all participant. Serum and plasma were isolated. Biochemical parameters were evaluated in serum. Plasma was used to measure malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC) levels, as well as superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity. Also, IL-6 level was investigated in plasma using ELISA method. Results: MDA and TOS levels were significantly higher in T2DM patients than the control group (p < 0.05). However, TAC and SOD were significantly lower in T2DM as compared with healthy subjects (p < 0.05). Also, IL-6 level was higher in T2DM in comparison to healthy subjects (p = 0.004). Furthermore, there was a significant positive correlation between IL-6 with MDA (p = 0.031, r = 0.482) and TOS (p < 0.001, r = 0.744). In addition, a negative correlation was observed between IL-6 and SOD activity (p = 0.002, r = −0.660). Conclusion: Reducing oxidative stress and inflammation could be effective in improvement of T2DM. [ABSTRACT FROM AUTHOR]

A simple flow injection analysis (FIA) integrating with a metal-free approach for total antioxidant capacity (TAC) was developed. The non-toxic reaction was based on generating a vibrant blue radical from imipramine to avoid the potential interferents arising from the colorful fruit extracts. The blue radical can be rapidly scavenged by antioxidant compounds present in the sample. TAC values of Thai tropical fruit extracts were assessed by monitoring the quenching in absorbance of the test mixture following the addition of the antioxidant compounds/fruit extracts. The FIA co-operated in order to increase the sample throughput. The results demonstrated that Antidesma thwaiteaianum Muell. Arg. has the highest capacity followed by Terminalia chebula Retz. and Phyllanthus Emblica Linn., respectively. An excellent correlation between the proposed method was found with the DPPH assay. The proposed method allowed the TAC determination of fruit extracts in a high-throughput and straightforward way in accordance with the principles of green analytical chemistry. [ABSTRACT FROM AUTHOR]

The study was conducted to investigate the effect of diets containing 0, 2.5, 5, 7.5, and 10% S. platensis meal on total antioxidant capacity (TAC) and lipid peroxidation, as well as the expression of two antioxidant enzyme genes (SOD and CAT) in rainbow trout (Oncorhynchus mykiss). One hundred and eighty fish, with an average initial weight of 101 ± 8 g, were cultured for 10 weeks. At the end of experiment, lipid peroxidation significantly decreased in serum of fish fed with S. platensis and fish fed with 5, 7.5, and 10% microalgae showed a significantly lower value compared to control and 2.5%. Inclusion of 7.5 and 10% S. platensis in diet also decreased lipid peroxidation in liver. TAC significantly increased with increasing level of S. platensis. Expression level of superoxide dismutase (SOD) and catalase (CAT) genes significantly increased in the fish liver after administration of microalgae and fish fed with 10% S. platensis in diet showed the highest level compared to the other treatments. The present study reveals that inclusion of 10% S. platensis in diet can decrease oxidative stress in rainbow trout. Therefore, S. platensis can be used as potential antioxidant for fish farming. [ABSTRACT FROM AUTHOR]

Background: Oxidative stress is considered to be involved in pathogenesis of different diseases including Major Depressive Disorder (MDD). In laboratory, this can be measured by a biochemical parameter called Oxidative stress Index (OSI), which can be calculated as the ratio percentage of Total peroxide to the total anti-oxidant potential. Aims and Objectives: This study was undertaken to determine OSI in MDD and healthy control and to find out whether any significant difference exists among the mean values of OSI in MDD and healthy controls. It was also aimed to find out whether OSI can be correlated with the severity of MDD. The validity of OSI as a biochemical parameter to diagnose MDD was also evaluated. Materials and Methods: In this study OSI was determined in 101 cases of Major Depressive Disorder (MDD) along with 106 age and sex matched controls by measuring Total Peroxides (TP) and Total Antioxidant Capacity (TAC). TP was measured by (FOX 2) method and TAC was measured by The Ferric Reducing Ability of Plasma (FRAP) method. The correlation of OSI & severity of depression was assessed by Spearman rank Correlation test. ROC curve was used for determining the validity of OSI for diagnosis of Depression. Result: Significant increase in OSI was observed in MDD (2.33 + 0.457) when compared to healthy controls (1.311 + 0.352). The increase was also found to be associated with severity of MDD (Spearman coefficient of rank correlation, rho = 0.289). The diagnostic ability of OSI was evaluated by ROC curve, which showed Area under curve as 0.96. The optimal cut off value of OSI was found to be 1.83, with 87.13% sensitivity and 92.45% specificity. Conclusion: Oxidative stress may play a role in pathogenesis of MDD as indicated by measuring OSI. This parameter is found to be significantly associated with severity of disease. The diagnostic ability of OSI for MDD is quite satisfactory. However, further study is needed to validate this finding.

Inflammation and pain induced by delayed onset muscle soreness (DOMS) can be induced by eccentric exercise or an unaccustomed activity. The condition can causes problems in exercising and for athletes. The purpose of this study was to assess the effect of 150mg curcumin supplementation immediately after intensive eccentric exercise. Evaluations were made for total antioxidant Capacity (TAC), muscle damage markers, and DOMS induced pain. Ten healthy young males (age, 25.0 ± 1.6 years; height, 178.9 ± 4.1 cm; body mass, 81.1 ± 6.8 kg; fat%, 14.2 ± 2.1) completed a double blind randomized-controlled crossover trial to estimate the effects of oral curcumin supplementation (150mg) and a placebo on squat performance and DOMS following unaccustomed heavy eccentric exercise. Curcumin (CU) or placebo (P) was taken at the prescribed dose immediately after eccentric squat exercises; administrations were separated by a 14-day washout period. Measurements were made at the baseline, immediately, 24, 48, and 72h after exercise comprising: (a) limb pain (1–10 cm visual analogue scale; VAS), (b) total antioxidant capacity (TAC) (c) serum markers of muscle damage and inflammation. Measurements taken after exercise showed significantly reduced levels of pain, creatine kinase (CK), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in C group compared with group P group (P

Abstract The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL−); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin(r)+S. typhimurium (TOYO); and CloSTATTM+ S. typhimurium (CLOS). Birds in all treatments, except for CTL−, were orally challenged with 3 x109 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen peroxide (H2O2) values were similar among all groups. The antibody titers against S. typhimurium and Newcastle disease (ND) were not affected by the treatments (p>0.05). However, S. typhimurium and ND titers were influenced by the interaction between week and treatment (p

Background: Periodontal inflammation causes an increased production of reactive oxidative stress leading to the production of reactive oxidative species, which causes tissue damage. Various studies have shown that Melatonin hormone is a potent antioxidant. Aim: To assess and compare the total antioxidant capacity and melatonin hormone level in saliva of healthy women, women with chronic periodontitis in reproductive and postmenopausal age groups, so as to render a comprehensive treatment in future. Materials and Methods: Total of 80 women were selected and divided into 4 groups. 20 aged 24-35 yr as group A with GI

Oxidative stress, caused by an imbalance between reactive oxygen species and antioxidants, is related to many dermatologic diseases. Increased reactive oxygen species is also associated with various decreased T‐cell immune responses. The incidence and severity of herpes zoster (HZ), which is caused by the reactivation of varicella‐zoster virus, increase with age because of declining cell‐mediated immunity. The main purpose of this study was to assess the levels of oxidative stress biomarkers in patients with HZ compared with control subjects. In this case‐control study, the serum levels of total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index, glutathione, superoxide dismutase, and total polyphenol content (TPC) in 43 patients with HZ and 47 age‐matched controls were determined, and their biomarker patterns were compared. TAC and TPC levels were significantly lower in patients with HZ; however, TOS and oxidative stress index levels were significantly higher in comparison with the control (P < .001). In addition, a significantly strong negative correlation was found between TAC and TPC with TOS levels in patients with HZ (r = −.79, P < .001; r = −.81, P < .001, respectively). Our findings showed an oxidative stress imbalance in HZ. Whether this change correlates with HZ pathogenesis or is a consequence of the inflammatory response to HZ needs more investigation. [ABSTRACT FROM AUTHOR]

Infertility is an overall issue that portrayed by multifactorial etiology including physiological, hereditary, irresistible, ecological factors and in addition autoimmunity. The goal of this investigation is the connection between the aggregate cell reinforcement limit level with specific cytokines; interleukin-6 (IL-6) and interferon gamma (IFN-γ) levels in fundamental liquid and blood of idiopathic Infertility and ripe male relationship with original liquid level. Blood and fundamental liquids were gathered from 70 guys with idiopathic Infertility and 20 males with demonstrated Infertility and were broke down for the level of TAC, IL-6, IFN-γ and original liquid quality. Fundamental TAC levels were p<0.05 and lower in those with idiopathic Infertility. A negative relationship was seen between sperm quality parameters and TAC levels in idiopathic Infertility men. A fascinating noteworthy height in serum and semen IFN-γ level was recognized in idiopathic Infertility men. Furthermore, altogether lifted serum and semen IL-6 level was likewise seen in a gathering of idiopathic Infertility men. Inconclusion, TAC level has a critical negative impact on sperm quality and subsequently adds to male Infertility. Novel cytokine miniaturized scale designs for patients with various sorts of Infertility may be proposed and hence, cytokine level estimation of IL-6 and IFN-γ in fundamental plasma is recommended to be incorporated into assessment of particular kinds of male Infertility. [ABSTRACT FROM AUTHOR]

Nocturia is a common symptom which causes sleeping disorders in the elderly. A number of studies have demonstrated that acupuncture may modulate a wide range of neuro endocrinological factors following stimulation of acupoints. We aim to examine the effect of acupuncture treatment and plasma levels of ADH, melatonin hormone and total antioxidant capacity (TAC) on nocturia patients. 28 healthy women subjects (Group I) and 27 women nocturia patients (Group II) have been joined to our study. Following a total of 10 acupuncture sessions has been applied with two sessions per week, we took blood samples from the nocturia patients (Group III). Plasma antidiuretic hormone, melatonin and TAC levels were studied by Elisa method. As a result, all of the patients responded to acupuncture treatment positively. In our study, patients with nocturnal polyuria (Group II) showed significantly low plasma antidiuretic hormone and melatonin levels in comparison to the Group I (p<0.001). Although plasma melatonin levels showed an increase by 12.35% in Group III in comparison to Group II, plasma ADH levels did not change between the two groups. Thus, it was concluded that melatonin could be beneficial for nocturia via central nervous system effect. Patients with nocturnal polyuria (Group 11) plasma TAC level showed a decrease when compared with the control group (Group I). However, the plasma TAC level decreased by 9.83% following the 10th session in Group III in comparison to Group II (p=0,044). Based on our results we concluded that acupuncture treatment has a regulatory effect on plasma melatonin levels in patients with nocturia and can be used in the treatment of nocturia patients. [ABSTRACT FROM AUTHOR]

Background: Sperm differentiation during spermatogenesis is highly regulated by different caspases and oxidant-antioxidant mechanisms. Objective: To assess the levels of the caspase -3 and total antioxidant capacity (TAC) in seminal plasma and evaluate its role in the pathogenesis of idiopathic infertile male in comparison with healthy individuals. Patients and Methods: Seminal fluids were collected from seventy males with idiopathic infertility and twenty male with proven fertility and were analyzed for the level of caspase -3 and TAC in seminal fluid and their correlation with seminal fluid quality parameters. Results: The results revealed that men with idiopathic infertility have significantly higher level of caspase-3 activity. Seminal TAC levels were significantly lower in those with idiopathic infertility compared with fertile men. A negative correlation was noticed between sperm quality parameters and TAC levels in idiopathic infertile men. Conclusion: Increased apoptotic index and decreased TAC activity are two important markers in idiopathic male infertility. [ABSTRACT FROM AUTHOR]

Recently, we reported a positive correlation between Klotho, as an anti-aging protein, and the total antioxidant capacity (TAC) in cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. However, there is no information about the Klotho and TAC changes within the central nervous system (CNS). Thus, the current study aimed to employ an experimental autoimmune encephalomyelitis (EAE) model in C57BL/6 mice using MOG35-55 peptide to examine the relationship between Klotho and TAC within the CNS. To this end, the brain and spinal cord were obtained at the onset and peak stages of EAE as well as non-EAE mice (sham/control groups). The Klotho expression was assessed in the brain and spinal cord of different experimental groups at mRNA (qPCR) and protein (ELISA) levels. Also, TAC level was determined in the tissues of different experimental groups. The results showed that Klotho expression in the brain at the onset and peak stages of EAE were significantly lower than that in non-EAE mice. Conversely, Klotho expression in the spinal cord at the onset of EAE was significantly higher than that of non-EAE mice, while Klotho was comparable at the peak stage of EAE and non-EAE mice. The pattern of TAC alteration in the brain and spinal cord of EAE mice was similar to that of Klotho expression. In conclusion, for the first time, this study demonstrated a significant positive correlation between Klotho and TAC changes during the pathogenesis of EAE. It is suggested that Klotho may have neuroprotective activity through the regulation of redox system. [ABSTRACT FROM AUTHOR]

This study aims to examine and compare the phenolic profile and antioxidant properties of two kinds of honey: Stingless bee honey (Kelulut honey) and Tualang honeys. Using liquid chromatography mass spectrometry, a total of eighteen phenolic acids and flavonoids have been identified in Kelulut honey samples. The phenolic content and total antioxidant capacity (three assays: DPPH, ABTS, and ORAC) for Kelulut and Tualang honeys were evaluated and statistically estimated. There was a significant ( p < 0.05) phenolic content (228.09 ± 7.9–235.28 ± 0.6 mg gallic acid equivalent per kg) and flavonoid content (97.88 ± 10.1–101.5 ± 11.4 mg catechin equivalent per kg) in Kelulut honey samples. The Kelulut honey samples have demonstrated a significantly stronger antioxidant capacity than Tualang honey samples. The correlations between antioxidant results and polyphenols content were found to be statistically significant ( P < 0.05, P < 0.01). This research is the first to report data on phenolic profile and total antioxidant capacity of Kelulut honey. Our data suggest that Kelulut honey has prominent medical properties and could be exploited as a natural nutraceutical to treat free radical associated diseases. [ABSTRACT FROM AUTHOR]

Background: Oxidative stress has been shown to be the most significant influential factor in cancer pathogenesis. Follicular cells are affected in papillary thyroid carcinoma (PTC), which is the most prevalent thyroid cancer associated with oxidative stress. As a noninvasive source of body metabolism, saliva has recently attracted the attention of researchers as an investigative specimen. The present study aimed to evaluate and compare the levels of total antioxidant capacity (TAC) and malondialdehyde (MDA) in the blood and saliva samples of PTC patients and healthy control subjects. Methods: This case-control study was conducted on the patients with PTC referring to Taleghani Hospital in Tehran, Iran. Age- and gender-matched healthy volunteers were selected as the control group. Blood and saliva samples were obtained from all the subjects. Measurement of theMDAand TAC levels was performed using a commercial kit (ZellBio GmbH, Germany) based on colorimetric methods. Data analysis was performed in MedCalc software version 14.8.1. Results: In total, 33 PTC patients and 33 healthy subjects were enrolled in the study with the mean age of 34.6 ±8.02 years. No statistically significant differences were observed in the demographic characteristics of the participants. Serum TAC andMDAlevels were significantly lowerand higher in the PTC group (P< 0.0001) compared to the control group (P = 0.0009), respectively. However, salivary TAC (P = 0.48) and MDA (P = 0.25) revealed no significant differences between the study groups. Conclusions: According to the results, PTC patients had oxidant/antioxidant imbalance, which could increase the risk of PTC. Furthermore, there were no significant differences in the TAC andMDAlevels measured in the saliva of the PTC patients and control subjects. This finding could be attributed to the ultrafiltration in plasma, which involves the seeping-through of the plasma molecules, not allowing the use of saliva as a substitute for serum or plasma. [ABSTRACT FROM AUTHOR]

Nanozyme–based colorimetric assays have aroused extensive attention in biosensing due to quick response, low cost and simplicity. However, their practical applications are limited by the unsatisfactory stability and catalytic activity of nanozymes in complex detection environments. Herein, using the one–pot chemical vapor deposition method, we successfully prepare a highly efficient and stable carbon supported Co–Ir nanozyme (termed as Co–Ir/C nanozyme) for the determination of total antioxidant capacity (TAC) in food samples. The Co–Ir/C nanozyme shows excellent durability under extensive pH ranges, high temperature and high salt concentration due to the protection of carbon supporter. It can be recycled by simple magnetic separation, and its catalytic activity remains stable after long–term operation and storage. Taking full advantage of the superior peroxidase–like activity of Co–Ir/C nanozyme, it is used for colorimetric detection of ascorbic acid (or known as vitamin C), an important vitamin to adjust body's normal physiological function, with results showing higher sensitivity (detection limit of 0.27 μM) than most of the recently published works. Moreover, the determination of TAC in vitamin C tablets and fruits are further achieved, which are in good agreement with those of commercial colorimetric test kits. This study helps guide the rational preparation of versatile and highly stable nanozymes, and develops a robust TAC determination platform for future food quality monitoring. [ABSTRACT FROM AUTHOR]