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5. Erratum: The Italian national project of astrobiology-life in space-origin, presence, persistence of life in space, from molecules to extremophiles (Astrobiology (2020) 20:5 (580-582) DOI: 10.1089/ast.2020.2247)

Author

Onofri S., Balucani N., Barone V., Benedetti P., Billi D., Balbi A., Brucato J. R., Cobucci-Ponzano B., Costanzo G., Rocca N. L., Moracci M., Saladino R., Vladilo G., Albertini N., Battistuzzi M., Bloino J., Botta L., Casavecchia P., Cassaro A., Claudi R., Cocola L., Coduti A., Di Donato P., Di Mauro E., Dore L., Falcinelli S., Fulle M., Ivanovski S., Lombardi A., Mancini G., Maris M., Maurelli L., Murante G., Negri R., Pacelli C., Pagano I., Piccinino D., Poletto L., Prantera G., Puzzarini C., Rampino S., Ripa C., Rosi M., Sanna M., Selbmann L., Silva L., Skouteris D., Strazzulli A., Tasinato N., Timperio A. M., Tozzi A., Tozzi G. P., Trainotti L., Ugliengo P., Vaccaro L., Zucconi L., Onofri S., Balucani N., Barone V., Benedetti P., Billi D., Balbi A., Brucato J.R., Cobucci-Ponzano B., Costanzo G., Rocca N.L., Moracci M., Saladino R., Vladilo G., Albertini N., Battistuzzi M., Bloino J., Botta L., Casavecchia P., Cassaro A., Claudi R., Cocola L., Coduti A., Di Donato P., Di Mauro E., Dore L., Falcinelli S., Fulle M., Ivanovski S., Lombardi A., Mancini G., Maris M., Maurelli L., Murante G., Negri R., Pacelli C., Pagano I., Piccinino D., Poletto L., Prantera G., Puzzarini C., Rampino S., Ripa C., Rosi M., Sanna M., Selbmann L., Silva L., Skouteris D., Strazzulli A., Tasinato N., Timperio A.M., Tozzi A., Tozzi G.P., Trainotti L., Ugliengo P., Vaccaro L., Zucconi L., Onofri, S., Balucani, N., Barone, V., Benedetti, P., Billi, D., Balbi, A., Brucato, J. R., Cobucci-Ponzano, B., Costanzo, G., Rocca, N. L., Moracci, M., Saladino, R., Vladilo, G., Albertini, N., Battistuzzi, M., Bloino, J., Botta, L., Casavecchia, P., Cassaro, A., Claudi, R., Cocola, L., Coduti, A., Di Donato, P., Di Mauro, E., Dore, L., Falcinelli, S., Fulle, M., Ivanovski, S., Lombardi, A., Mancini, G., Maris, M., Maurelli, L., Murante, G., Negri, R., Pacelli, C., Pagano, I., Piccinino, D., Poletto, L., Prantera, G., Puzzarini, C., Rampino, S., Ripa, C., Rosi, M., Sanna, M., Selbmann, L., Silva, L., Skouteris, D., Strazzulli, A., Tasinato, N., Timperio, A. M., Tozzi, A., Tozzi, G. P., Trainotti, L., Ugliengo, P., Vaccaro, L., and Zucconi, L.

Subjects
life in space-origin, astrobiology, persistence of life in space, from molecules to extremophiles in space, Settore CHIM/02 - Chimica Fisica
Abstract

The Italian National Project of Astrobiology―Life in Space―Origin, Presence, Persistence of Life in Space, from Molecules to Extremophiles A member’s name should be added to the OPPS Project Team: Stavro Ivanovski on page 580 footnotes. We have made this correction on the title page of the article and the eTOC.

Published
2020

10. Aspetti applicativi della transgenesi nei sistemi vegetali

Author

Lanfranco L, Trainotti L., DI SANSEBASTIANO, Gian Pietro, Pasqua G, Cozzolino S, Di Sansebastiano GP, Forni C, Genre A, Lanfranco L, Miccheli A, Trainotti L, Valletta A., Lanfranco, L, DI SANSEBASTIANO, Gian Pietro, and Trainotti, L.

Published
2011

11. Ingegneria genetica nelle piante

Author

Trainotti L, Lanfranco L, DI SANSEBASTIANO, Gian Pietro, Pasqua G, Cozzolino S, Di Sansebastiano GP, Forni C, Genre A, Lanfranco L, Miccheli A, Trainotti L, Valletta A., Trainotti, L, Lanfranco, L, and DI SANSEBASTIANO, Gian Pietro

Published
2011

14. Transcriptional regulatory networks controlling woolliness in peach in response to preharvest gibberellin application and cold storage

Author

PEGORARO, C., TADIELLO, A., GIRARDI, C. L., CHAVES, F. C., QUECINI, V., OLIVEIRA, A. C. de, TRAINOTTI, L., ROMBALDI, C. V., CESAR LUIS GIRARDI, CNPUV, and VERA MARIA QUECINI, CNPUV.

Subjects
Genome, Prunus Persica, cold storage, Chilling injury, Gene expression, Gibberellic acid
Abstract

Background: Postharvest fruit conservation relies on low temperatures and manipulations of hormone metabolism to maintain sensory properties. Peaches are susceptible to chilling injuries, such as ?woolliness? that is caused by juice loss leading to a ?wooly? fruit texture. Application of gibberellic acid at the initial stages of pit hardening impairs woolliness incidence, however the mechanisms controlling the response remain unknown. We have employed genome wide transcriptional profiling to investigate the effects of gibberellic acid application and cold storage on harvested peaches. Results: Approximately half of the investigated genes exhibited significant differential expression in response to the treatments. Cellular and developmental process gene ontologies were overrepresented among the differentially regulated genes, whereas sequences in cell death and immune response categories were underrepresented. Gene set enrichment demonstrated a predominant role of cold storage in repressing the transcription of genes associated to cell wall metabolism. In contrast, genes involved in hormone responses exhibited a more complex transcriptional response, indicating an extensive network of crosstalk between hormone signaling and low temperatures. Time course transcriptional analyses demonstrate the large contribution of gene expression regulation on the biochemical changes leading to woolliness in peach. Conclusion: Overall, our results provide insights on the mechanisms controlling the complex phenotypes associated to postharvest textural changes in peach and suggest that hormone mediated reprogramming previous to pit hardening affects the onset of chilling injuries. Keywords: Chilling injury, Cold storage, Genome wide gene expression, Gibberellic acid, Prunus persica

Published
2015

17. The Italian Initiative for peach miRNA analysis (miRNitAly)

Author

VERDE I., FACCIOLI P., ROSSINI L., BONGHI C., RASORI A., Di SERIO F., TRAINOTTI L., GISEL A., LAZZARI B., VENDRAMIN E., COLAIACOVO M., STELLA A., RAMINA A., and CATTIVELLI L.

Subjects
peach genomics, miRNAs, plant-virus and plant-viroid interaction, plant-virus and plant viroid interaction, siRNAs
Published
2010

29. EFFECT OF SILENCING OF CELL WALL DEGRADING ENZYMES ON STRAWBERRY FRUIT SOFTENING

Author

Quesada, M.A., primary, Posé, S., additional, Santiago-Doménech, N., additional, Sesmero, R., additional, Molina, M.C., additional, Mousa, S., additional, Pliego-Alfaro, F., additional, Mercado, J.A., additional, Caballero, J.L., additional, Muñoz-Blanco, J., additional, Trainotti, L., additional, Casadoro, G., additional, García-Gago, J.A., additional, and López-Aranda, J.M., additional

Published
2009
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38. Characterization of abscission zones in the flowers and fruits of peach [<em>Prunus persica</em> (L.) Batsch].

Author

Zanchin, A., Marcato, C., Trainotti, L., Casadoro, G., and Rascio, N.

Subjects
ABSCISSION (Botany), DEFOLIATION, PRUNUS, ALMOND, FRUIT, CELLS
Abstract

Cell wall hydrolases their mRNAs, and ultrastructural details of cell wall digestion have been studied in peach abscission zones (AZ) located at the base of flower bud (AZ1) and the base of flower receptacle (AZ2 ), respectively, Induction of abscission was obtain by treatment of explants with exogenous ethylene. Cell separation pattern of the two examined abscission zones have been compared with those of other already known AZs of peach, i.e. the AZ3 located between fruit and peduncle and the leaf AZ. Analyses have shown similarities in response to ethylene treatment between AZ1 and leaf AZ and between AZ2 and AZ3 on the flower bud . The timing of functional differentiation, evaluated as th cells ability to respond to induction by ethylene treatment showed that AZ1 and AZ2 became functional after bud breaking and bud scale shedding. Later on, they lost their functionality about 6-7 wk from anthesis. AZ3 became functional very precoiously and could be activated 1 wk after anthesis in the fertilized flowers. In the latter zone the cell could also undergo a morphological prddifferentiation, even though it occurred a long time after the acquisition of the ethylene responsiveness. This finding shows that morphologicaI differntiation is not necessrily a perequiste for those cell to become competent to respond to the abscission inducing stimuli. [ABSTRACT FROM AUTHOR]

Published
1995
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40. Cellulase and cell separation

Author

Casadoro, G., Ferrarese, L., Trainotti, L., Moretto, P., and Rascio, N.

Abstract

The involvement of cellulase (endo-b-l,4-glucanase, EC 3.2.1.4) in a number of different cell separation events which occur in higher plants has been well established. Besides their significance for the plant growth and differentiation, these events can be economically important since they also comprise softening of fleshy fruits and abscission of fruits, flowers and leaves. In higher plants cellulase is present in a number of different biochemical isoforms which are encoded by different genes. This finding is in accordance with the wide range of physiological events which require the intervention of cellulase activity, and whose peculiarities and amplitudes can be quite various.

Published
1995
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46. The Italian National Project of Astrobiology—Life in Space—Origin, Presence, Persistence of Life in Space, from Molecules to Extremophiles

Author

Onofri, Silvano, Balucani, Nadia, Barone, Vincenzo, Benedetti, Pietro, Billi, Daniela, Balbi, Amedeo, Brucato, John Robert, Cobucci-Ponzano, Beatrice, Costanzo, Giovanna, La Rocca, Nicoletta, Moracci, Marco, Saladino, Raffaele, Vladilo, Giovanni, Niccolò, Albertini, Mariano, Battistuzzi, Julien, Bloino, Lorenzo, Botta, Piergiorgio, Casavecchia, Alessia, Cassaro, Riccardo, Claudi, Lorenzo, Cocola, Alberto, Coduti, Paola Di Donato, Ernesto Di Mauro, Luca, Dore, Stefano, Falcinelli, Marco, Fulle, Stavro, Ivanovski, Andrea, Lombardi, Giordano, Mancini, Michele, Maris, Luisa, Maurelli, Giuseppe, Murante, Rodolfo, Negri, Claudia, Pacelli, Isabella, Pagano, Davide, Piccinino, Luca, Poletto, Giorgio, Prantera, Cristina, Puzzarini, Sergio, Rampino, Caterina, Ripa, Marzio, Rosi, Monica, Sanna, Laura, Selbmann, Laura, Silva, Dimitrios, Skouteris, Andrea, Strazzulli, Nicola, Tasinato, Anna Maria Timperio, Andrea, Tozzi, Gian Paolo Tozzi, Trainotti, Livio, Piero, Ugliengo, Luigi, Vaccaro, and Laura Zucconi, Onofri, S., Balucani, N., Barone, V., Benedetti, P., Billi, D., Balbi, A., Brucato, J. R., Cobucci-Ponzano, B., Costanzo, G., Rocca, N. L., Moracci, M., Saladino, R., Vladilo, G., Albertini, N., Battistuzzi, M., Bloino, J., Botta, L., Casavecchia, P., Cassaro, A., Claudi, R., Cocola, L., Coduti, A., Di Donato, P., Di Mauro, E., Dore, L., Falcinelli, S., Fulle, M., Ivanovski, S., Lombardi, A., Mancini, G., Maris, M., Maurelli, L., Murante, G., Negri, R., Pacelli, C., Pagano, I., Piccinino, D., Poletto, L., Prantera, G., Puzzarini, C., Rampino, S., Ripa, C., Rosi, M., Sanna, M., Selbmann, L., Silva, L., Skouteris, D., Strazzulli, A., Tasinato, N., Timperio, A. M., Tozzi, A., Tozzi, G. P., Trainotti, L., Ugliengo, P., Vaccaro, L., Zucconi, L., Onofri S., Balucani N., Barone V., Benedetti P., Billi D., Balbi A., Brucato J.R., Cobucci-Ponzano B., Costanzo G., Rocca N.L., Moracci M., Saladino R., Vladilo G., Albertini N., Battistuzzi M., Bloino J., Botta L., Casavecchia P., Cassaro A., Claudi R., Cocola L., Coduti A., Di Donato P., Di Mauro E., Dore L., Falcinelli S., Fulle M., Ivanovski S., Lombardi A., Mancini G., Maris M., Maurelli L., Murante G., Negri R., Pacelli C., Pagano I., Piccinino D., Poletto L., Prantera G., Puzzarini C., Rampino S., Ripa C., Rosi M., Sanna M., Selbmann L., Silva L., Skouteris D., Strazzulli A., Tasinato N., Timperio A.M., Tozzi A., Tozzi G.P., Trainotti L., Ugliengo P., Vaccaro L., and Zucconi L.

Subjects
Persistence (psychology), Extremophiles, Exobiology, Extraterrestrial Environment, Origin of Life, Origin of Life in Space, Space (commercial competition), Astrobiology, Life in Space, From Molecules to Extremophiles, Astrobiology, Abiogenesis, From Molecules to Extremophiles, Extremophile, Life in Space, Origin of life, space, exobiology, News and Views, extremophiles, Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali, Settore FIS/05, Chemistry, space, Agricultural and Biological Sciences (miscellaneous), Space and Planetary Science, astrobiology, from Molecules to Extremophiles in space
Abstract

THE ''LIfe in Space'' project was funded in the wake of the Italian Space Agency's proposal for the development of a network of institutions and laboratories conceived to implement Italian participation in space astrobiology experiments. Of primary concern for this project is the study of the origin of life in the Universe, a focus that will promote investigation into prebiotic chemistry in various possible scenarios, whether in polar or nonpolar solvents (e.g., Titan's environment). Such results will link with study of the effects of simulated space conditions on possible chemical bio- signatures. The limits of life as we know it will be investigated in ground-based experiments with microorganisms that have already demonstrated their resistance to extreme environments on Earth and to real or simulated space conditions. The potential survival of microorganisms will also be examined with up-to-date molecular methods. The ability of some microorganisms to produce atmospheric and surface biosignatures when exposed to simulated conditions will be tested and compared with the possible existence of bio- signatures on potentially habitable exoplanets. Furthermore, the search for potentially habitable exoplanets, with space- based observational methods, will be optimized by way of dedicated climate models with the capacity to predict the detectability of atmospheric biosignatures for a broad range of planetary conditions. The project embraces the four most important topics in astrobiological research, as listed below, along with relevant contributions from the participating Italian institutions. Origins and evolution of organic compounds of biological significance in space (comets, asteroids, rocky planets, and moons); Prebiotic syntheses, origin of life, and early life; The limits of life and biological habitability: origin, evolution and adaptation of life in extreme environments on Earth and in space; Biomarkers for life detection in the Solar System and on exoplanets.

Published
2020
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47. Impact of tree training system, branch type and position in the canopy on the ripening homogeneity of 'Abbé Fétel' pear fruit

Author

Abdur Rahim, Livio Trainotti, Guglielmo Costa, A. Jajo, Sara Serra, Nethal K. Jajo, Federico Gagliardi, Claudio Bonghi, Stefano Musacchi, Jajo, A., Mda, Rahim, Serra, S., Gagliardi, F., Jajo, N.K., Musacchi, S., Costa, G., Bonghi, C., and Trainotti, L.

Subjects
Canopy, PEAR, Partial least square path modelling, Homogeneity (statistics), Partial least square discriminate analysis, food and beverages, Forestry, Ripening, Pyrus communis, Horticulture, Biology, Storage management, biology.organism_classification, Homogeneous, Botany, Gene expression, Pyrus communis, ripening indices, Partial Least Square Discriminate Analysis, Partial Least Square Path Modelling, Genetics, Gene expression, Molecular Biology
Abstract

In pear, fruit ripening is not homogeneous, which leads to problems in harvest and storage management. To identify factors affecting the ripening homogeneity, structural, biochemical and molecular parameters were investigated. Fruits were sampled from trees trained with three different systems, spindle, V-shaped and bi-axis, and were further grouped on the basis of canopy position (top or bottom) and branch type (short-old-spurs, 3-year-old or older branches, 2-year-old branches and twigs). This study confirmed that the position in the canopy plays a crucial role in fruit ripening in V-shaped and bi-axis training systems. Fruit on the spindle training system was unaffected by the position of the canopy, albeit showing a large variability within the fruit of each part of the canopy. Such variability is much lower in the bi-axis and V-shaped systems. The spindle training system had no similarity in homogeneity indices between the molecular and physiological measurements. Partial least square discriminate analysis (PLS-DA) showed differences in training system, branch type and canopy position separated by the different physiological and molecular parameters. Short-old-spurs and twigs had the highest homogeneity and least variability in all training systems and branch types. In addition, partial least square path modelling (PLS-PM) was able to identify a high correlation between physiological measurements and ripening compared to that of molecular data.

Published
2014

48. I vacuoli

Author

DI SANSEBASTIANO, Gian Pietro, Forni C., Pasqua G, Cozzolino S, Di Sansebastiano GP, Forni C, Genre A, Lanfranco L, Miccheli A, Trainotti L, Valletta A., DI SANSEBASTIANO, Gian Pietro, and Forni, C.

Published
2011

49. Bioproduzioni e biomasse

Author

Forni C, DI SANSEBASTIANO, Gian Pietro, Pasqua G, Cozzolino S, Di Sansebastiano GP, Forni C, Genre A, Lanfranco L, Miccheli A, Trainotti L, Valletta A., Forni, C, and DI SANSEBASTIANO, Gian Pietro

Published
2011

50. Il Sistema di endomembrane

Author

DI SANSEBASTIANO, Gian Pietro, Pasqua G, Cozzolino S, Di Sansebastiano GP, Forni C, Genre A, Lanfranco L, Miccheli A, Trainotti L, Valletta A., and DI SANSEBASTIANO, Gian Pietro

Published
2011

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