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29 results on '"Trichet, Léa"'

2. Topotactic fibrillogenesis of freeze-casted microridged collagen scaffolds for 3D cell culture

Author

Rieu, Clément, Mosser, Gervaise, Haye, Bernard, Coradin, Thibaud, Fernandes, Francisco M., and Trichet, Léa

Subjects
Physics - Biological Physics, Condensed Matter - Soft Condensed Matter, Quantitative Biology - Tissues and Organs
Abstract

Type I collagen is the main component of the extra-cellular matrix (ECM). In vitro, under a narrow window of physico-chemical conditions, type I collagen self-assembles to form complex supramolecular architectures reminiscent of those found in native ECM. Presently, a major challenge in collagen-based biomaterials is to couple the delicate collagen fibrillogenesis events with a controlled shaping process in non-denaturating conditions. In this work an ice-templating approach promoting the structuration of collagen into macroporous monoliths is used. Instead of common solvent removal procedures, a new topotactic conversion approach yielding self-assembled ordered fibrous materials is implemented. These collagen-only, non-cross-linked scaffolds exhibit uncommon mechanical properties in the wet state. With the help of the ice-patterned micro-ridge features, Normal Human Dermal Fibroblasts and C2C12 murine myoblasts successfully migrate and form highly-aligned populations within the resulting 3D biomimetic collagen scaffolds. These results open a new pathway to the development of new tissue engineering scaffolds ordered across various organization levels from the molecule to the macropore, and are of particular interest for biomedical applications where large scale 3D cell alignment is needed such as for muscular or nerve reconstruction., Comment: 34 pages, 7 figures, 4 supporting information figures

Published
2018

3. Biomimetic tubular materials: from native tissues to a unifying view of new vascular, tracheal, gastrointestinal, oesophageal, and urinary grafts.

Author

Martinier, Isabelle, Trichet, Léa, and Fernandes, Francisco M.

Subjects
*BIOMIMETIC materials, *EPITHELIUM, *GASTROINTESTINAL system, *URINARY organs, *BIOMATERIALS
Abstract

Repairing tubular tissues—the trachea, the esophagus, urinary and gastrointestinal tracts, and the circulatory system—from trauma or severe pathologies that require resection, calls for new, more effective graft materials. Currently, the relatively narrow family of materials available for these applications relies on synthetic polymers that fail to reproduce the biological and physical cues found in native tissues. Mimicking the structure and the composition of native tubular tissues to elaborate functional grafts is expected to outperform the materials currently in use, but remains one of the most challenging goals in the field of biomaterials. Despite their apparent diversity, tubular tissues share extensive compositional and structural features. Here, we assess the current state of the art through a dual layer model, reducing each tissue to an inner epithelial layer and an outer muscular layer. Based on this model, we examine the current strategies developed to mimic each layer and we underline how each fabrication method stands in providing a biomimetic material for future clinical translation. The analysis provided here, addressed to materials chemists, biomaterials engineers and clinical staff alike, sets new guidelines to foster the elaboration of new biomimetic materials for effective tubular tissue repair. [ABSTRACT FROM AUTHOR]

Published
2025
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18. Core–Shell Pure Collagen Threads Extruded from Highly Concentrated Solutions Promote Colonization and Differentiation of C3H10T1/2 Cells

Author

Picaut, Lise, primary, Trichet, Léa, additional, Hélary, Christophe, additional, Ducourthial, Guillaume, additional, Bonnin, Marie-Ange, additional, Haye, Bernard, additional, Ronsin, Olivier, additional, Schanne-Klein, Marie-Claire, additional, Duprez, Delphine, additional, Baumberger, Tristan, additional, and Mosser, Gervaise, additional

Published
2021
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27. Activité et réponse à une blessure d’un tapis de cellules

Author

Buguin, Axel, primary, Grasland, Erwan, additional, Petitjean, Laurence, additional, Poujade, Mathieu, additional, Reffay, Myriam, additional, Silberzan, Pascal, additional, du Roure, Olivia, additional, Ghibaudo, Marion, additional, Ladoux, Benoît, additional, Saez, Alexandre, additional, and Trichet, Léa, additional

Published
2010
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29. Activité et réponse à une blessured’un tapis de cellules

Author

Buguin, Axel, Grasland, Erwan, Petitjean, Laurence, Poujade, Mathieu, Reffay, Myriam, Silberzan, Pascal, du Roure, Olivia, Ghibaudo, Marion, Ladoux, Benoît, Saez, Alexandre, and Trichet, Léa

Abstract

La migration et l’adhésion cellulaires sont deux processus essentiels intervenant dans la structuration et le développement des tissus.Pour préciser l’influence respective des environnements biochimiques et mécaniques sur la cellule permettant cette organisation, nous présentons deux expériences utilisant des dispositifs microfabriqués.L’une permet de mesurer les forces que des cellules exercent sur leur substrat, et l’autre permet de déterminer la réponse d’un tissu à une blessure modèle.Les résultats mettent en évidence une réponse spécifique des cellules en fonction de leur environnement mécanique local.

Published
2010

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