1. Isolation, characterization and management of endophyte(s) prevailing during the in vitro establishment of endangered medicinal herb Himalayan Trillium (Trillium govanianum Wall. ex D. Don)
- Author
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Chaudhary, Damini, Kumar, Vinay, Sharma, Rajnish, Devi, Sunita, Sharma, Parul, and Kumar, Pankaj
- Abstract
Endophytes are the microorganisms (fungi or bacteria) that usually live within plant tissues without showing symptoms of disease. In most cases, these endophytes exhibit a symbiotic relationship with the host plant, but in tissue culture procedures, these endophytes overgrow in established cultures, thus hindering the practice of in vitro establishment. Such endophytic growth, which mainly involves the growth of endophytic bacteria, has an undesirable impact on the in vitro establishment of the endangered medicinal herb Himalayan Trillium (Trillium govanianum). Therefore, this study aimed at the isolation, characterization, and management of such endophyte(s) prevalent during in vitro establishment. Tryptic soy agar has been reported to be a suitable medium for the growth of endophytic bacteria as it supports the growth of a wide range of bacteria. Five endophytic bacteria were identified, namely Pseudomonas migulae UHF-TGN4 (Accession no. OP518462), Agrobacterium radiobacter UHF-TGNRH (Accession no. OP518463), Enterobacter hormaechei subsp. hoffmannii UHF-TGTSR (Accession no. OP518464), Pseudomonas umsongensis UHF-TGTS5 (Accession no. OP518467) and Pseudomonas kielensis UHF-TG8RH (Accession no. OP518468) were identified based on morphological, biochemical and molecular characterization. These isolates were deposited at the National Centre for Microbial Resource, Pune, Maharashtra, India for further maintenance. Various antibiotics, such as streptomycin, amikacin, kanamycin, gentamicin, tetracycline, cefotaxime, cotrimoxazole, and ampicillin were used to study their effectiveness in controlling bacterial growth. Out of these, three antibiotics namely, streptomycin, cefotaxime and tetracycline were selected on the basis of their efficacy for zone of inhibition. The antibiotic cefotaxime at a concentration of 120 mg/L was found effective during the in vitro establishment practices. To our knowledge, this is the first report on the identification and management of endophytic bacteria during in vitro establishment Himalayan Trillium. These findings could help in maintaining healthy in vitro cultures of this valuable and endangered medicinal herb for its mass propagation and conservation aspects.Key message: This is the first report on identification and management of endophytic bacteria during in vitro establishment of endangered medicinal herb Trillium govanianum (Himalayan Trillium). The findings would be helpful in maintaining healthy in vitro cultures of this valuable endangered medicinal herb for its mass propagation and conservation aspects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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