1. Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
- Author
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Renee A Pond, Laila Hussaini, Benjamin A. Pinsky, Colleen S. Kraft, Victoria Stittleburg, Youssef Saklawi, Malaya K. Sahoo, Evan J. Anderson, Jesse J. Waggoner, Nadine Rouphael, and Ahmed Babiker
- Subjects
Epidemiology ,Expedited ,viruses ,rRT-PCR ,coronavirus ,lcsh:Medicine ,medicine.disease_cause ,0302 clinical medicine ,Nasopharynx ,030212 general & internal medicine ,skin and connective tissue diseases ,Coronavirus ,biology ,Clinical performance ,virus diseases ,3. Good health ,Reverse transcription polymerase chain reaction ,molecular detection ,Infectious Diseases ,Real-time polymerase chain reaction ,coronavirus disease ,severe acute respiratory syndrome coronavirus 2 ,Microbiology (medical) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,030231 tropical medicine ,Real-Time Polymerase Chain Reaction ,2019 novel coronavirus disease ,lcsh:Infectious and parasitic diseases ,Betacoronavirus ,respiratory infections ,03 medical and health sciences ,Multiplex polymerase chain reaction ,Research Letter ,medicine ,Humans ,lcsh:RC109-216 ,SARS ,Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 ,SARS-CoV-2 ,business.industry ,lcsh:R ,fungi ,COVID-19 ,biology.organism_classification ,Virology ,zoonoses ,body regions ,business ,Multiplex Polymerase Chain Reaction ,Coronavirus Infections - Abstract
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2–3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
- Published
- 2020
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