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Your search keyword '"Trujillo-Roldán, Mauricio A."' showing total 212 results
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212 results on '"Trujillo-Roldán, Mauricio A."'

8. A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures.

Author

Reynoso-Cereceda, Greta I., Valdez-Cruz, Norma A., Pérez, Nestor O., and Trujillo-Roldán, Mauricio A.

Subjects
ESCHERICHIA coli, GRANULOCYTE-macrophage colony-stimulating factor, RECOMBINANT proteins, AXIAL flow, CELLULAR inclusions
Abstract

The effect of gradients of elevated glucose and low dissolved oxygen in the addition zone of fed-batch E. coli thermoinduced recombinant high cell density cultures can be evaluated through two-compartment scale-down models. Here, glucose was fed in the inlet of a plug flow bioreactor (PFB) connected to a stirred tank bioreactor (STB). E. coli cells diminished growth from 48.2 ± 2.2 g/L in the stage of RP production if compared to control (STB) with STB-PFB experiments, when residence time inside the PFB was 25 s (34.1 ± 3.5 g/L) and 40 s (25.6 ± 5.1 g/L), respectively. The recombinant granulocyte-macrophage colony-stimulating factor (rHuGM-CSF) production decreased from 34 ± 7% of RP in inclusion bodies (IB) in control cultures to 21 ± 8%, and 7 ± 4% during the thermoinduction production phase when increasing residence time inside the PFB to 25 s and 40 s, respectively. This, along with the accumulation of acetic and formic acid (up to 4 g/L), indicates metabolic redirection of central carbon routes through metabolic flow and mixed acid fermentation. Special care must be taken when producing a recombinant protein in heat-induced E. coli, because the yield and productivity of the protein decreases as the size of the bioreactors increases, especially if they are carried at high cell density. HIGHLIGHTS: Thermoinduced recombinant E. coli grew less in a two-compartment scale-down model. Heat-inducible E. coli cultures at a large scale significantly decrease recombinant protein production. The accumulation of acetic and formic acid increases when E. coli is exposed to glucose and oxygen gradients. The axial flow pattern inside the PFB mimics glucose and dissolved oxygen gradients at the industrial scale. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Assembly collapsing versus heterozygosity oversizing: detection of homokaryotic and heterokaryotic Laccaria trichodermophora strains by hybrid genome assembly

Author

Ángeles-Argáiz, Rodolfo Enrique, primary, Aguirre-Beltrán, Luis Fernando Lozano, additional, Hernández-Oaxaca, Diana, additional, Quintero-Corrales, Christian, additional, Trujillo-Roldán, Mauricio A., additional, Castillo-Ramírez, Santiago, additional, and Garibay-Orijel, Roberto, additional

Published
2024
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18. Integrative overview of antibodies against SARS-CoV-2 and their possible applications in COVID-19 prophylaxis and treatment

Author

Valdez-Cruz, Norma A., García-Hernández, Enrique, Espitia, Clara, Cobos-Marín, Laura, Altamirano, Claudia, Bando-Campos, Carlos G., Cofas-Vargas, Luis F., Coronado-Aceves, Enrique W., González-Hernández, Ricardo A., Hernández-Peralta, Pablo, Juárez-López, Daniel, Ortega-Portilla, Paola A., Restrepo-Pineda, Sara, Zelada-Cordero, Patricio, and Trujillo-Roldán, Mauricio A.

Published
2021
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28. Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli

Author

Restrepo-Pineda, Sara, primary, Rosiles-Becerril, Diego, additional, Vargas-Castillo, Angélica B., additional, Ávila-Barrientos, Luis P., additional, Luviano, Axel, additional, Sánchez-Puig, Nuria, additional, García-Hernández, Enrique, additional, Pérez, Nestor O., additional, Trujillo-Roldán, Mauricio A., additional, and Valdez-Cruz, Norma A., additional

Published
2022
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30. Computational Design of Inhibitors Targeting the Catalytic β Subunit of Escherichia coli FOF1-ATP Synthase

Author

Avila-Barrientos, Luis Pablo, primary, Cofas-Vargas, Luis Fernando, additional, Agüero-Chapin, Guillermin, additional, Hernández-García, Enrique, additional, Ruiz-Carmona, Sergio, additional, Valdez-Cruz, Norma A., additional, Trujillo-Roldán, Mauricio, additional, Weber, Joachim, additional, Ruiz-Blanco, Yasser B., additional, Barril, Xavier, additional, and García-Hernández, Enrique, additional

Published
2022
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32. Computational Design of Inhibitors Targeting the Catalytic β Subunit of Escherichia coli FO F₁-ATP Synthase

Author

Avila-Barrientos, Luis Pablo, Cofas-Vargas, Luis Fernando, Agüero-Chapin, Guillermin, Hernández-García, Enrique, Ruiz-Carmona, Sergio, Valdez-Cruz, Norma A., Trujillo-Roldán, Mauricio, Weber, Joachim, Ruiz-Blanco, Yasser B., Barril, Xavier, and García-Hernández, Enrique

Subjects
Medizin
Abstract

With the uncontrolled growth of multidrug-resistant bacteria, there is an urgent need to search for new therapeutic targets, to develop drugs with novel modes of bactericidal action. FoF1-ATP synthase plays a crucial role in bacterial bioenergetic processes, and it has emerged as an attractive antimicrobial target, validated by the pharmaceutical approval of an inhibitor to treat multidrug-resistant tuberculosis. In this work, we aimed to design, through two types of in silico strategies, new allosteric inhibitors of the ATP synthase, by targeting the catalytic β subunit, a centerpiece in communication between rotor subunits and catalytic sites, to drive the rotary mechanism. As a model system, we used the F1 sector of Escherichia coli, a bacterium included in the priority list of multidrug-resistant pathogens. Drug-like molecules and an IF1-derived peptide, designed through molecular dynamics simulations and sequence mining approaches, respectively, exhibited in vitro micromolar inhibitor potency against F1. An analysis of bacterial and Mammalia sequences of the key structural helix-turn-turn motif of the C-terminal domain of the β subunit revealed highly and moderately conserved positions that could be exploited for the development of new species-specific allosteric inhibitors. To our knowledge, these inhibitors are the first binders computationally designed against the catalytic subunit of FOF1-ATP synthase. CA extern

Published
2022

34. List of contributors

Author

Agunbiade, Mayowa, Arunima, Aziziyan, Fatemeh, Bhardwaj, Nisha, Bhat, Sarita G., Cascelli, Nicoletta, Castilla-Marroquin, Jesus D., Dabirmanesh, Bahareh, De Chiaro, Addolorata, Farzam, Farnoosh, Guebitz, Georg M., Hernández-Martínez, Ricardo, Hernández-Rosas, Francisco, Jose, Jiya, Khajeh, Khosro, Khera, Khushi, Kudanga, Tukayi, Le Roes-Hill, Marilize, Lettera, Vincenzo, Mehrotra, Rekha, Michel, Hamy, Nemadziva, Blessing, Nyanhongo, Gibson S., Parra-Saldivar, Roberto, Prins, Alaric, Rathod, Virendra K., Romero-Martínez, Daniel, Sajeevan, Shilja, Sannia, Giovanni, Trujillo-Roldán, Mauricio A., Valdez-Cruz, Norma A., Verma, Preeti, Weiss, Renate, Yadav, Aarti, and Yadav, Deepti

Published
2024
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38. Additional file 1 of Integrative overview of antibodies against SARS-CoV-2 and their possible applications in COVID-19 prophylaxis and treatment

Author

Valdez-Cruz, Norma A., García-Hernández, Enrique, Espitia, Clara, Cobos-Marín, Laura, Altamirano, Claudia, Bando-Campos, Carlos G., Cofas-Vargas, Luis F., Coronado-Aceves, Enrique W., González-Hernández, Ricardo A., Hernández-Peralta, Pablo, Juárez-López, Daniel, Ortega-Portilla, Paola A., Restrepo-Pineda, Sara, Zelada-Cordero, Patricio, and Trujillo-Roldán, Mauricio A.

Subjects
body regions, viruses, fungi, skin and connective tissue diseases, respiratory tract diseases
Abstract

Additional file 1: Table S1. Participation of the immune system in the infection by SARS-CoV-2. Table S2. Summary of outcomes regarding the use of convalescent plasma from COVID-19 patients. Table S3. Binding affinity of monoclonal antibodies that block or neutralize interaction between SARS-CoV-2 and hACE2. Table S4. Clinical evaluation of mAbs against SARS-CoV-2. Table S5. Binding affinity of nanobodies that block or neutralize interaction between SARS-CoV-2 and hACE2. Table S6. Process and product related potential critical quality attributes (pCQAs) to be taken into account for the production of anti-SARS-CoV-2 mAbs to obtain a pure active pharmaceutical ingredient.

Published
2021
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40. Compartmentalized Proteomic Profiling Outlines the Crucial Role of the Classical Secretory Pathway during Recombinant Protein Production in Chinese Hamster Ovary Cells

Author

Pérez-Rodriguez, Saumel, Wulff, Tune, Voldborg, Bjørn G, Altamirano, Claudia, Trujillo-Roldán, Mauricio A, Valdez-Cruz, Norma A, Pérez-Rodriguez, Saumel, Wulff, Tune, Voldborg, Bjørn G, Altamirano, Claudia, Trujillo-Roldán, Mauricio A, and Valdez-Cruz, Norma A

Abstract

Different cellular processes that contribute to protein production in Chinese hamster ovary (CHO) cells have been previously investigated by proteomics. However, although the classical secretory pathway (CSP) has been well documented as a bottleneck during recombinant protein (RP) production, it has not been well represented in previous proteomic studies. Hence, the significance of this pathway for production of RP was assessed by identifying its own proteins that were associated to changes in RP production, through subcellular fractionation coupled to shot-gun proteomics. Two CHO cell lines producing a monoclonal antibody with different specific productivities were used as cellular models, from which 4952 protein groups were identified, which represent a coverage of 59% of the Chinese hamster proteome. Data are available via ProteomeXchange with identifier PXD021014. By using SAM and ROTS algorithms, 493 proteins were classified as differentially expressed, of which about 80% was proposed as novel targets and one-third were assigned to the CSP. Endoplasmic reticulum (ER) stress, unfolded protein response, calcium homeostasis, vesicle traffic, glycosylation, autophagy, proteasomal activity, protein synthesis and translocation into ER lumen, and secretion of extracellular matrix components were some of the affected processes that occurred in the secretory pathway. Processes from other cellular compartments, such as DNA replication, transcription, cytoskeleton organization, signaling, and metabolism, were also modified. This study gives new insights into the molecular traits of higher producer cells and provides novel targets for development of new sub-lines with improved phenotypes for RP production.

Published
2021

43. Computational Design of Inhibitors Targeting the Catalytic β Subunit of Escherichia coli F O F 1 -ATP Synthase.

Author

Avila-Barrientos, Luis Pablo, Cofas-Vargas, Luis Fernando, Agüero-Chapin, Guillermin, Hernández-García, Enrique, Ruiz-Carmona, Sergio, Valdez-Cruz, Norma A., Trujillo-Roldán, Mauricio, Weber, Joachim, Ruiz-Blanco, Yasser B., Barril, Xavier, and García-Hernández, Enrique

Subjects
ESCHERICHIA coli, MOLECULAR dynamics, MULTIDRUG-resistant tuberculosis, ADENOSINE triphosphatase, BACTERICIDAL action, KINASE inhibitors, RIFAMPIN
Abstract

With the uncontrolled growth of multidrug-resistant bacteria, there is an urgent need to search for new therapeutic targets, to develop drugs with novel modes of bactericidal action. FoF1-ATP synthase plays a crucial role in bacterial bioenergetic processes, and it has emerged as an attractive antimicrobial target, validated by the pharmaceutical approval of an inhibitor to treat multidrug-resistant tuberculosis. In this work, we aimed to design, through two types of in silico strategies, new allosteric inhibitors of the ATP synthase, by targeting the catalytic β subunit, a centerpiece in communication between rotor subunits and catalytic sites, to drive the rotary mechanism. As a model system, we used the F1 sector of Escherichia coli, a bacterium included in the priority list of multidrug-resistant pathogens. Drug-like molecules and an IF1-derived peptide, designed through molecular dynamics simulations and sequence mining approaches, respectively, exhibited in vitro micromolar inhibitor potency against F1. An analysis of bacterial and Mammalia sequences of the key structural helix-turn-turn motif of the C-terminal domain of the β subunit revealed highly and moderately conserved positions that could be exploited for the development of new species-specific allosteric inhibitors. To our knowledge, these inhibitors are the first binders computationally designed against the catalytic subunit of FOF1-ATP synthase. [ABSTRACT FROM AUTHOR]

Published
2022
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49. Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis

Author

Pérez-Rodriguez, Saumel, de Jesús Ramírez-Lira, María, Wulff, Tune, Voldbor, Bjørn Gunnar, Ramírez, Octavio T., Trujillo-Roldán, Mauricio A., Valdez-Cruz, Norma A., Pérez-Rodriguez, Saumel, de Jesús Ramírez-Lira, María, Wulff, Tune, Voldbor, Bjørn Gunnar, Ramírez, Octavio T., Trujillo-Roldán, Mauricio A., and Valdez-Cruz, Norma A.

Abstract

Chinese hamster ovary cells have been the workhorse for the production of recombinant proteins in mammalian cells. Since biochemical, cellular and omics studies are usually affected by the lack of suitable fractionation procedures to isolate compartments from these cells, differential and isopycnic centrifugation based techniques were characterized and developed specially for them. Enriched fractions in intact nuclei, mitochondria, peroxisomes, cis-Golgi, trans-Golgi and endoplasmic reticulum (ER) were obtained in differential centrifugation steps and subsequently separated in discontinuous sucrose gradients. Nuclei, mitochondria, cis-Golgi, peroxisomes and smooth ER fractions were obtained as defined bands in 30–60% gradients. Despite the low percentage represented by the microsomes of the total cell homogenate (1.7%), their separation in a novel sucrose gradient (10–60%) showed enough resolution and efficiency to quantitatively separate their components into enriched fractions in trans-Golgi, cis-Golgi and ER. The identity of these organelles belonging to the classical secretion pathway that came from 10–60% gradients was confirmed by proteomics. Data are available via ProteomeXchange with identifier PXD019778. Components from ER and plasma membrane were the most frequent contaminants in almost all obtained fractions. The improved sucrose gradient for microsomal samples proved being successful in obtaining enriched fractions of low abundance organelles, such as Golgi apparatus and ER components, for biochemical and molecular studies, and suitable for proteomic research, which makes it a useful tool for future studies of this and other mammalian cell lines.

Published
2020

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