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8. Trends in the susceptibility of methicillin-resistant Staphylococcus aureus to nine antimicrobial agents, including ceftobiprole, nemonoxacin, and tyrothricin: results from the Tigecycline In Vitro Surveillance in Taiwan (TIST) study, 2006–2010

Author

Chen, Y.-H., Liu, C.-Y., Ko, W.-C., Liao, C.-H., Lu, P.-L., Huang, C.-H., Lu, C.-T., Chuang, Y.-C., Tsao, S.-M., Chen, Y.-S., Liu, Y.-C., Chen, W.-Y., Jang, T.-N., Lin, H.-C., Chen, C.-M., Shi, Z.-Y., Pan, S.-C., Yang, J.-L., Kung, H.-C., Liu, C.-E., Cheng, Y.-J., Liu, J.-W., Sun, W., Wang, L.-S., Yu, K.-W., Chiang, P.-C., Lee, M.-H., Lee, C.-M., Hsu, G.-J., and Hsueh, P.-R.

Published
2014
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18. Comparative Analysis of Two Commercial Automated Systems with Agar Dilution for Oxacillin Susceptibility and Their Association with Genotypes of Invasive Staphylococcus aureus Isolates (2011–2021)

Author

Wang WY, Chen YH, Lee YL, Chiu CF, and Tsao SM

Subjects
oxacillin susceptibility, automated systems, agar dilution, sccmec, ca-mrsa, Infectious and parasitic diseases, RC109-216
Abstract

Wei-Yao Wang,1,2 Yi-Hsin Chen,3– 5 Yu-Lin Lee,1,2 Chen-Feng Chiu,6 Shih-Ming Tsao1,2 1School of Medicine, Chung Shan Medical University, Taichung, Taiwan; 2Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan; 3Department of Nephrology, Taichung Tzu Chi Hospital, Taichung, Taiwan; 4School of Medicine, Tzu Chi University, Hualien, Taiwan; 5Department of Artificial Intelligence and Data Science, National Chung Hsing University, Taichung, Taiwan; 6Department of Internal Medicine, Feng Yuan Hospital, Ministry of Health and Welfare, Taichung, TaiwanCorrespondence: Shih-Ming Tsao, Department of Internal Medicine, Chung Shan Medical University Hospital, &num; 110, Section 1, Jianguo North Road, Taichung City, 40201, Taiwan, Tel +886 4 24739595 ext.34718, Fax +886-4-23248172, Email tsmhwy@ms24.hinet.netBackground: Determining oxacillin susceptibility using reference methods and automated systems is crucial for treating invasive infections caused by Staphylococcus aureus. This study compares the oxacillin susceptibility results from the two automated systems with agar dilution and correlates them with genotypes of invasive S. aureus.Methods: Non-duplicate S. aureus invasive isolates were collected over an 11-year period. The oxacillin susceptibility was determined with Phoenix 100 (Jan 2011 to Aug 2018) or Vitek 2 (Sep 2018 to Dec 2021), and susceptibility for oxacillin and cefoxitin was determined with agar dilution. Methicillin-resistant S. aureus (MRSA) was confirmed with mecA existence, and the genotype was determined using SCCmec. The association between genotype and antibiotic susceptibility using two automated systems and agar dilution was evaluated.Results: A total of 842 invasive S. aureus, including 443 mecA+ MRSA and 399 mecA- MSSA, were collected. The susceptibility rates of oxacillin determined by two automated systems and agar dilution were 68.8% (76.8% for Phoenix 100 and 57.6% for Vitek 2) and 54.0%, respectively. When compared with the oxacillin susceptibility using agar dilution, the categorical agreement for Phoenix 100 and Vitek 2 were 0.46% and 0.88%, respectively (p < 0.001). One hundred and forty-three isolates were misinterpreted as oxacillin-susceptible S. aureus (OSSA) using automated systems while comparing with agar dilution, among which molecularly community-associated MRSA (CA-MRSA) outnumbered healthcare-associated MRSA (HA-MRSA) (99 vs 34, p < 0.001). There were 70 mecA+ OSSA (OS-MRSA) using agar dilution, among which 42 harbored SCCmec types were predominantly categorized as CA-MRSA (38, p < 0.001).Conclusion: The categorical agreement of Vitek 2 in determining oxacillin susceptibility and predicting mecA existence is comparable with agar dilution, whereas Phoenix 100 is not. Most of those ORSA determined by agar dilution but misinterpreted as OSSA by automated systems and OS-MRSA are categorized as CA-MRSA.Keywords: oxacillin susceptibility, automated systems, agar dilution, SCCmec, CA-MRSA

Published
2024

28. Multicentre evaluation of magnetic technology for localisation of non-palpable breast lesions and targeted axillary nodes

Author

McCamley, C, Ruyssers, N, To, H, Tsao, S, Keane, H, Poliness, C, Mehta, K, Rose, A, Baker, C, Mann, GB, McCamley, C, Ruyssers, N, To, H, Tsao, S, Keane, H, Poliness, C, Mehta, K, Rose, A, Baker, C, and Mann, GB

Abstract

BACKGROUND: Magseed technology is a recently introduced localisation technique for impalpable breast lesions with possible advantages over traditional techniques. These include improved theatre logistics, flexibility in incision placement and improved patient experience. This multicentre study evaluates the experience of introducing Magseed technology into routine surgical practice. METHODS: A prospective multicentre study of Magseed localised procedures was performed. Insertion data were recorded by the radiologist including lesion characteristics and Magseed insertion accuracy. The surgical team recorded time from insertion to operation, operating time and surgical satisfaction. Pathology results were reviewed for specimen weight and margins. RESULTS: Between February 2019 and June 2020, 100 patients were enrolled. Magseed localised procedures included 18 excisional biopsies, 23 wide local excisions (WLE), 50 WLE with axillary surgery and four cases of Magseed localised breast WLE with Magseed localised axillary surgery. There were three therapeutic mammoplasties and two cases of Magseed localised targeted axillary node dissection alone. A total of 90% of Magseeds were radiologically placed within 5 mm of the target lesion/node. Time between incision and specimen removal was 17 min (range 6-40 min). All breast and axillary Magseeds were successfully identified and retrieved during surgery. The target lesion was identified in the specimen in all cases. A total of 10% of cases required further surgery for pathologically positive margins. Overall, surgeons reported that Magseed localisation was "easy" or "very easy" in 77% of cases. CONCLUSION: Magseed is a reliable, safe and accurate surgical technique that provides logistical advantages and flexibility of surgical approach. The method was well-accepted by all users.

Published
2021

41. Managing cyber security vulnerabilities in large networks

Author

Chang, Edward S., Jain, Aridaman K., Slade, David M., and Tsao, S. Lee

Subjects
Lucent Technologies Inc., Intranets -- Safety and security measures, Telecommunications equipment industry, Network management systems -- Safety and security measures, Computer network equipment industry, Science and technology, Telecommunications industry
Abstract

Methods involving statistical analysis are combined with network and host security discipline in order to develop Lucent Technologies' cyber security profile. Lucent's patent-pending methodologies for enabling cyber security management in large networks could eventually be as important to network security as quality control charts are to manufacturing processes.

Published
1999

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