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6. The Chemicals between Us--First Results of the Cluster Analyses on Anatomy Embalming Procedures in the German-Speaking Countries

Author

Kerner, Alexander Michael, Biedermann, Uta, Bräuer, Lars, Caspers, Svenja, Doll, Sara, Engelhardt, Maren, Filler, Timm J., Ghebremedhin, Estifanos, Gundlach, Stefanie, Hayn-Leichsenring, Gregor U., Heermann, Stephan, Hettwer-Steeger, Ingrid, Hiepe, Laura, Hirt, Bernhard, Hirtler, Lena, Hörmann, Rom, Kulisch, Christoph, Lange, Tobias, Leube, Rudolf, Meuser, Annika Hela, Müller-Gerbl, Magdalena, Nassenstein, Christina, Neckel, Peter H., Nimtschke, Ute, Paulsen, Friedrich, Prescher, Andreas, Pretterklieber, Michael, Schliwa, Stefanie, Schmidt, Katja, Schmiedl, Andreas, Schomerus, Christof, Schulze-Tanzil, Gundula, Schumacher, Udo, Schumann, Sven, Spindler, Volker, Streicher, Johannes, Tschernig, Thomas, Unverzagt, Axel, Valentiner, Ursula, Viebahn, Christoph, Wedel, Thilo, Weigner, Janet, Weninger, Wolfgang J., Westermann, Jürgen, Weyers, Imke, Waschke, Jens, and Hammer, Niels

Abstract

Hands-on courses utilizing preserved human tissues for educational training offer an important pathway to acquire basic anatomical knowledge. Owing to the reevaluation of formaldehyde limits by the European Commission, a joint approach was chosen by the German-speaking anatomies in Europe (Germany, Austria, Switzerland) to find commonalities among embalming protocols and infrastructure. A survey comprising 537 items was circulated to all anatomies in German-speaking Europe. Clusters were established for "ethanol"-, formaldehyde-based ("FA"), and "other" embalming procedures, depending on the chemicals considered the most relevant for each protocol. The logistical framework, volumes of chemicals, and infrastructure were found to be highly diverse between the groups and protocols. Formaldehyde quantities deployed per annum were three-fold higher in the "FA" (223 L/a) compared to the "ethanol" (71.0 L/a) group, but not for "other" (97.8 L/a), though the volumes injected per body were similar. "FA" was strongly related to table-borne air ventilation and total fixative volumes =1000 L. "Ethanol" was strongly related to total fixative volumes >1000 L, ceiling- and floor-borne air ventilation, and explosion-proof facilities. Air ventilation was found to be installed symmetrically in the mortuary and dissection facilities. Certain predictors exist for the interplay between the embalming used in a given infrastructure and technical measures. The here-established cluster analysis may serve as decision supportive tool when considering altering embalming protocols or establishing joint protocols between institutions, following a best practice approach to cater toward best-suited tissue characteristics for educational purposes, while simultaneously addressing future demands on exposure limits.

Published
2023
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22. High-Affinity Lectin Ligands Enable the Detection of Pathogenic Pseudomonas aeruginosaBiofilms: Implications for Diagnostics and Therapy

Author

Zahorska, Eva, Denig, Lisa Marie, Lienenklaus, Stefan, Kuhaudomlarp, Sakonwan, Tschernig, Thomas, Lipp, Peter, Munder, Antje, Gillon, Emilie, Minervini, Saverio, Verkhova, Varvara, Imberty, Anne, Wagner, Stefanie, and Titz, Alexander

Abstract

Pseudomonas aeruginosais a critical priority pathogen and causes life-threatening acute and biofilm-associated chronic infections. The choice of suitable treatment for complicated infections requires lengthy culturing for species identification from swabs or an invasive biopsy. To date, no fast, pathogen-specific diagnostic tools for P. aeruginosainfections are available. Here, we present the noninvasive pathogen-specific detection of P. aeruginosausing novel fluorescent probes that target the bacterial biofilm-associated lectins LecA and LecB. Several glycomimetic probes were developed to target these extracellular lectins and demonstrated to stain P. aeruginosabiofilms in vitro. Importantly, for the targeting of LecA an activity boost to low-nanomolar affinity could be achieved, which is essential for in vivoapplication. In vitro, the nanomolar divalent LecA-targeted imaging probe accumulated effectively in biofilms under flow conditions, independent of the fluorophore identity. Investigation of these glycomimetic imaging probes in a murine lung infection model and fluorescence imaging revealed accumulation at the infection site. These findings demonstrate the use of LecA- and LecB-targeting probes for the imaging of P. aeruginosainfections and suggest their potential as pathogen-specific diagnostics to accelerate the start of the appropriate treatment.

Published
2024
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25. Distribution of TRPC5 in the human lung: A study in body donors.

Author

UMLAUF, FREDERIK, DIEBOLT, COLINE M., ENGLISCH, COLYA N., FLOCKERZI, FIDELIS, and TSCHERNIG, THOMAS

Subjects
TRP channels, HEMATOXYLIN & eosin staining, ION channels, ALVEOLAR macrophages, HUMAN experimentation
Abstract

Transient receptor potential channel canonical 5 (TRPC5) is a non-selective ion channel; ion influx through TRPC5 causes activation of downstream signaling pathways. In addition, TRPC5 has been identified as having a potential role in pathological processes, particularly in diseases caused by cellular cation homeostasis dysregulation, such as bronchial asthma or pulmonary hypertension. However, the expression and distribution of TRPC5 in the human lung remain unclear. To date, TRPC5 has only been detected in a few cell types in the human lung, such as airway, pulmonary venous and arterial smooth muscle cells. The present study therefore aimed to investigate the protein expression of TRPC5 in the human lung and to evaluate its histological distribution. Human lung samples were obtained from six preserved body donors. After processing, both hematoxylin & eosin staining, as well as immunohistochemistry were performed. Microscopic analysis revealed medium to strong immunostaining signals in all lung structures examined, including the pleura, pulmonary arteries and veins, bronchioles, alveolar septa, type 1 and 2 pneumocytes, as well as alveolar macrophages. Current research suggests that TRPC5 may be involved in various pathological processes in the human lung and some pharmacological compounds have already been identified that affect the function of TRPC5. Therefore, TRPC5 may present a novel drug target for therapeutic intervention in various lung diseases. The results of the present study indicate that the TRPC5 protein is expressed in all examined histological structures of the human lung. These findings suggest that TRPC5 may be more important for physiological cell function and pathophysiological cell dysfunction in the lung than is currently known. Further research is needed to explore the role and therapeutic target potential of TRPC5 in the human lung. [ABSTRACT FROM AUTHOR]

Published
2024
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28. Experimental and virtual testing of bone-implant systems equipped with the AO Fracture Monitor with regard to interfragmentary movement

Author

Wickert, Kerstin, primary, Roland, Michael, additional, Andres, Annchristin, additional, Diebels, Stefan, additional, Ganse, Bergita, additional, Kerner, Dorothea, additional, Frenzel, Felix, additional, Tschernig, Thomas, additional, Ernst, Manuela, additional, Windolf, Markus, additional, Müller, Max, additional, Pohlemann, Tim, additional, and Orth, Marcel, additional

Published
2024
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33. Rare Superior and Middle Trunk Fusion Accompanied by Altered Division Rearrangement Results in a Unique Brachial Plexus Variant: A Case Report.

Author

Schlüter, Andreas Marco, Redl, Konstantin, Tschernig, Thomas, Maxeiner, Stephan, and Krasteva-Christ, Gabriela

Subjects
BRACHIAL plexus, CONSCIOUSNESS raising, MEDICAL students, MEDICAL cadavers, NERVE fibers
Abstract

During routine dissections of cadavers as part of the medical curriculum, we identified a rare unilateral variation in the brachial plexus on the right side of a female body donor. This variation consisted of four unusual changes to the regular pattering of nerve bundles and the dorsal scapular artery permeating the complex neural network. The variation included contributions of root C4 to the plexus by a root C4/C5 anastomosis, a rare fusion of the superior and middle trunks to a 'superomiddle' trunk, a preliminary, proximal branching of the suprascapular nerve off the C5 root. We further observed an accessory 'medial anterior division' branching off the fused upper and middle trunks merging with the anterior division of the inferior trunk forming the medial cord. The latter event potentially introduced nerve fibers from C5 to C7, which are absent in common patterns. We aim to relate these observations to previous categorizations and quantifications of brachial plexus patterns. We believe that the combination of different variations in this case resulted in a unique pattern. Since this observation was made in the dissection class, we further aim to raise awareness among medical students and anatomical instructors for the likelihood of variations to textbook patterns. This will hopefully foster an appreciation of uniqueness and individuality in the interaction with future patients demonstrating that proper preparation prior to surgical interventions is always a necessary prerequisite. [ABSTRACT FROM AUTHOR]

Published
2024
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49. Neutralizing Complement C5a Protects Mice with Pneumococcal Pulmonary Sepsis

Author

Müller-Redetzky, Holger, Kellermann, Ute, Wienhold, Sandra-Maria, Gutbier, Birgitt, Lienau, Jasmin, Hellwig, Katharina, Reppe, Katrin, Letsiou, Eleftheria, Tschernig, Thomas, Scholz, Markus, Ahnert, Peter, Maasch, Christian, Hoehlig, Kai, Klussmann, Sven, Vater, Axel, Firsching, Theresa C., Hoppe, Judith, Suttorp, Norbert, and Witzenrath, Martin

Published
2020
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