1. DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci.
- Author
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Liu Y, McGann CD, Krebs M, Perkins TA Jr, Fields R, Camplisson CK, Nwizugbo DZ, Hsu C, Avanessian SC, Tsue AF, Kania EE, Shechner DM, Beliveau BJ, and Schweppe DK
- Abstract
The accuracy of crucial nuclear processes such as transcription, replication, and repair, depends on the local composition of chromatin and the regulatory proteins that reside there. Understanding these DNA-protein interactions at the level of specific genomic loci has remained challenging due to technical limitations. Here, we introduce a method termed "DNA O-MAP", which uses programmable peroxidase-conjugated oligonucleotide probes to biotinylate nearby proteins. We show that DNA O-MAP can be coupled with sample multiplexed quantitative proteomics and next-generation sequencing to quantify DNA-protein and DNA-DNA interactions at specific genomic loci., Competing Interests: Competing Interest Statement D.K.S. is a collaborator with Thermo Fisher Scientific, Genentech, Calico Labs, and AI Proteins. C.K.C., A.F.T., E.K., D.M.S., and B.J.B. have filed a patent application covering aspects of this work. B.J.B. is listed as an inventor on patent applications related to the SABER technology related to this work.
- Published
- 2024
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