1. Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue
- Author
-
Pao Lin Kuo, Tsui Yu Tracy Hsieh, Yung Ming Lin, Yen Ni Teng, Yi Shing Lin, and Ying Hung Lin
- Subjects
Male ,Genetics ,education.field_of_study ,Tissue microarray ,Proteome ,Microarray ,Microarray analysis techniques ,Gene Expression Profiling ,Population ,Gene Expression ,Obstetrics and Gynecology ,Biology ,Gene expression profiling ,Reproductive Medicine ,Complementary DNA ,Testis ,Gene expression ,Humans ,Spermatogenesis ,education ,Gene ,Infertility, Male ,Azoospermia ,Oligonucleotide Array Sequence Analysis - Abstract
Objective To identify novel genes that are down-regulated in the testicular tissue of infertile men. Design Prospective study. Setting University-based reproductive clinics and genetics laboratory. Patients Nine patients with normal spermatogenesis, and 15 patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS). Intervention Testicular samples of patients with the same histology were pooled for complementary DNA (cDNA) microarray analysis. Main Outcome Measure Novel, down-regulated genes. Results In total, 300 genes were significantly down-regulated in SCOS or MA samples, and 10 novel sterility-related genes were identified. Of the 10 novel genes, 6 genes (Hs.126780, Hs.553658, Hs.274135, Hs.268122, Hs.531701, and Hs.171130) encode proteins with predictable functional domains, and all these functional domains are believed to correlate with spermatogenesis and/or spermiogenesis. Conversely, the other 4 genes (Hs.351582, Hs.407480, Hs.552781, and Hs.355570) do not encompass known functional domains. Two genes (Hs.407480 and Hs.552781) lack mouse orthologues. Most novel genes showed a testis-specific expression pattern in both mice and humans. Reverse transcription-polymerase chain reaction (RT-PCR) showed three distinct types of developmental stage-dependent expressions of message ribonucleic acid (mRNA) for these novel genes in murine testes. Conclusion These 10 novel genes provide targets to elucidate novel pathways involved in human spermatogenesis.
- Published
- 2006
- Full Text
- View/download PDF