1. Expression of rac1 Protein in the Crypt-Villus Axis of Rat Small Intestine: In Reference to Insulin Action
- Author
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Katsuhito Kawasaki, Chinatsu Oda, Tomonori Kurokawa, Tsuneko Yamamoto, Eriko Chono, and Sadahiko Ishibashi
- Subjects
Male ,medicine.medical_specialty ,RHOA ,medicine.medical_treatment ,Blotting, Western ,Crypt ,Biophysics ,digestive system ,Biochemistry ,Rat Small Intestine ,Diabetes Mellitus, Experimental ,GTP-Binding Proteins ,Immunoblot Analysis ,Internal medicine ,Intestine, Small ,medicine ,Animals ,Insulin ,Rats, Wistar ,Molecular Biology ,biology ,digestive, oral, and skin physiology ,Cell Biology ,Rats ,rac GTP-Binding Proteins ,Endocrinology ,RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1 ,biology.protein - Abstract
The expression of rho family along the crypt-villus axis of rat small intestine was investigated. The immunoblot analysis showed that the content of rac1 protein gradually increased from tip-villus to crypt-villus enterocytes without changes in the contents of rhoA and rac2 proteins. Furthermore, the contents of rac1 protein in the entire enterocytes were markedly decreased in streptozotocin-induced diabetic rats and restored by short-term treatment with insulin. These results suggest that differentiation and proliferation in the enterocytes of rat small intestine are possibly related to the expression of rac1 protein through insulin action.
- Published
- 1997
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