Search

Your search keyword '"Tucciarone, C. M."' showing total 28 results
Start Over Author "Tucciarone, C. M."
28 results on '"Tucciarone, C. M."'

16. EPIDEMIOLOGIA DI METAPNEUMOVIRUS AVIARE NEL POLLO DA CARNE IN ITALIA

Author

Tucciarone C. M., Franzo G., Lupini C., Torres Alejo C., Listorti V., Mescolini G., Brandão P. E., Martini M., Catelli E., Cecchinato M., SOCIETA' ITALIANA DI PATOLOGIA AVIARE, and Tucciarone C. M., Franzo G., Lupini C., Torres Alejo C., Listorti V., Mescolini G., Brandão P. E., Martini M., Catelli E., Cecchinato M.

Subjects
metapneumovirus, broilers, italy
Abstract

The poultry production sector is tightly centred in Northern Italy, where chickens, turkey and other minor species are reared closely. Among the several well-know and managed respiratory issues, avian Metapneumovirus (aMPV) is growing as a concern also in chicken production, especially in broiler farming. Consistent data on its prevalence are lacking, so aMPV detections were summarized from longitudinal studies and diagnostic one-off samplings from Northern Italy from 2014 to 2016. The results demonstrated the wide circulation of aMPV subtype B and its presence in subsequent cycles in the same farm. aMPV sequences from chickens and turkeys showed high homology from the comparison, excluding a host-based strain separation. A ield vaccination trail was also performed in two aMPV positive farms to verify the beneits of a subtype B-based vaccine administration in broilers, which appeared to fully control the clinical signs even if the persistence of a ield strain was revealed in one of the farms.

Published
2017

22. Insights on Avian Metapneumovirus Subtype B circulation in Europe

Author

Lupini, C., Mescolini, G., Franzo, G., Blanco, A., Biarnes, M., Cecchinato, M., Tucciarone, C. M., Felice, V., Silveira, F., Catelli, E., and C. Lupini, G. Mescolini, G. Franzo, A. Blanco, M. Biarnes, M. Cecchinato, C. Maria Tucciarone, V. Felice, F. Silveira, E. Catelli

Subjects
avian metapneumovirus, epidemiology, phylogenetic analysis
Published
2018

26. IMPATTO DI DIFFERENTI STRATEGIE VACCINALI SULLE DINAMICHE DI POPOLAZIONE DEL GENOTIPO QX DI BRONCHITE INFETTIVA E SULLA FREQUENZA DEI FOCOLAI

Author

Franzo, G., Tucciarone, C. M., Cecchinato, M., CATELLI, ELENA, Franzo, G., Tucciarone, C.M., Catelli, E., and Cecchinato, M.

Subjects
Bronchite infettiva, coronavirus, genotipo QX
Abstract

L’estrema variabilità e la rapida evoluzione del virus della bronchite infettiva (IBV) hanno sempre rappresentato la sfida principale per il suo controllo a causa della limitata cross-protezione fra i diversi ceppi. Vari studi sperimentali hanno dimostrato un incremento nello spettro di protezione qualora gli animali siano vaccinati con più genotipi differenti. Ciò non di meno, le condizioni e la tempistica di somministrazione della vaccinazione in campo sono a tal punto differenti che spesso una generalizzazione dei risultati sperimentali è quantomeno questionabile. Nel presente studio è stato utilizzato un approccio di tipo epidemiologico-filodinamico per ricostruire la storia demografica del principale genotipo di campo (i.e. genotipo QX) circolante in Italia. Centonovantacinque campioni raccolti nel periodo 2012-2016 sono stati sequenziati e analizzati. Ciò ha permesso di evidenziare come sia le dinamiche di popolazione di questo virus che la frequenza di focolai clinici siano state particolarmente variabili nel corso del tempo. Di particolare rilievo è risultata la forte associazione fra questi fenomeni e i cambiamenti nelle strategie vaccinali adottate. Ciò ha permesso di dimostrare il rapporto di causa-effetto fra la somministrazione/ sospensione del vaccino per IBV e le ripercussioni in termini di dimensione della popolazione virale e, soprattutto, dell’occorrenza di episodi clinici indotti da IBV. È stato quindi possibile fornire una robusta dimostrazione dell’effetto protettivo indotto della vaccinazione nella pratica routinaria di allevamento. Inoltre, il resoconto qui riportato evidenzia l’importanza di pianificare con rigore non solo le strategie di controllo ma anche il loro monitoraggio e la loro valutazione, evitando di affidarsi a pareri soggettivi ed estemporanei.

Published
2016

27. Evaluation of unintended 1/96 infectious bronchitis vaccine transmission in broilers after direct contact with vaccinated ones.

Author

PELLATTIERO, E., TUCCIARONE, C. M., FRANZO, G., BERTO, G., KOUTOULIS, K., MEINI, A., ZANGRANDI, C., RAMON, G., DRIGO, M., and CECCHINATO, M.

Subjects
*AVIAN infectious bronchitis virus, *COMMUNICABLE diseases, *EPIDEMIOLOGY, *ANIMAL vaccination, *VACCINES
Abstract

Infectious bronchitis virus is characterised by an extreme degree of variability which deeply affects the first-choice control strategies against the disease. Each country tends to adopt its own protocols and even vaccine producers themselves can also adopt different strategies in attempts to confront local epidemiological concerns. In the present study, we tested the potential environmental persistence, transmission ability and replication capability of a non-directly administrated vaccine strain at a hatchery and during transportation. To this purpose, we examined a single hatchery, where combined vaccination (Mass-like plus 793B-like strains) is commonly administered following the protectotype concept, whereas a single broiler flock receives only the Mass priming. Two groups of solely Mass-primed chicks were kept in contact with chicks vaccinated with both strains, during hatchery procedures and transportation, respectively. A regularly vaccinated control group was selected and all three were monitored by swab sampling until 11 days of age. Vaccine titres were quantified using vaccinespecific real-time RT-PCRs to check the kinetics of both strains. Mass titres were consistent among the groups, while the absence of the 1/96 vaccine strain in unvaccinated chicks confirmed the low risk of unintended vaccine transmission, which could complicate the diagnostic process and the epidemiological scenario. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

28. Evaluation of 793/B-like and Mass-like vaccine strain kinetics in experimental and field conditions by real-time RT-PCR quantification.

Author

Tucciarone, C M, Franzo, G, Berto, G, Drigo, M, Ramon, G, Koutoulis, K C, Catelli, E, and Cecchinato, M

Subjects
*AVIAN infectious bronchitis virus, *VACCINATION, *REVERSE transcriptase polymerase chain reaction, *VIRUS diseases, *PREDICATE calculus, *ECONOMICS
Abstract

Infectious bronchitis virus (IBV) is a great economic burden both for productive losses and costs of the control strategies. Many different vaccination protocols are applied in the same region and even in consecutive cycles on the same farm in order to find the perfect balance between costs and benefits. In Northern Italy, the usual second vaccination is more and more often moved up to the chick's first d of life. The second strain administration together with the common Mass priming by spray at the hatchery allows saving money and time and reducing animal stress. The present work compared the different vaccine strains (Mass-like or B48, and 1/96) kinetics both in field conditions and in a 21-day-long experimental trial in broilers, monitoring the viral replication by upper respiratory tract swabbing and vaccine specific real time reverse transcription PCR (RT-PCR) quantification. In both field and experimental conditions, titers for all the vaccines showed an increasing trend in the first 2 wk and then a decrease, though still remaining detectable during the whole monitored period. IBV field strain and avian Metapneumovirus (aMPV) presence also was also investigated by RT-PCR and sequencing, and by multiplex real-time RT-PCR, respectively, revealing a consistency in the pathogen introduction timing at around 30 d, in correspondence with the vaccine titer's main decrease. These findings suggest the need for an accurate knowledge of live vaccine kinetics, whose replication can compete with the other pathogen one, providing additional protection to be added to what is conferred by the adaptive immune response. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results