19 results on '"Tue, Ngo Tri"'
Search Results
2. A Clinical and Epidemiological Investigation of the First Reported Human Infection With the Zoonotic Parasite Trypanosoma evansi in Southeast Asia
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Van Vinh Chau, Nguyen, Chau, Le Buu, Desquesnes, Marc, Herder, Stephane, Lan, Nguyen Phu Huong, Campbell, James I., Van Cuong, Nguyen, Yimming, Benjarat, Chalermwong, Piangjai, Jittapalapong, Sathaporn, Franco, Jose Ramon, Tue, Ngo Tri, Rabaa, Maia A., Carrique-Mas, Juan, Thanh, Tam Pham Thi, Thieu, Nga Tran Vu, Berto, Alessandra, Hoa, Ngo Thi, Van Minh Hoang, Nguyen, Tu, Nguyen Canh, Chuyen, Nguyen Khac, Wills, Bridget, Hien, Tran Tinh, Thwaites, Guy E., Yacoub, Sophie, and Baker, Stephen
- Published
- 2016
3. Mobilising community-based research on zoonotic infections: A case study of longitudinal cohorts in Vietnam
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Karen Saylors, Tue Ngo Tri, Toan Tran Khanh, Kiet Bach Tuan, Heiman FL Wertheim, Stephen Baker, Hoa Ngo Thi, and Juliet E Bryant
- Subjects
Human settlements. Communities ,HT51-65 - Abstract
We initiated the Vietnam Initiative on Zoonotic Infections (VIZIONS) research program to better understand the epidemiology of disease transmission at the human–animal interface in Vietnamese rural communities and to integrate One Health approaches to disease surveillance. We established a longitudinal community cohort study of individuals with occupational exposure to animals, which involves concurrent targeted sampling from domestic livestock species and follow-up monitoring of human clinical cases. The project was implemented by government agencies at the provincial and district levels of the public health and animal health sectors in Vietnam. Engaging with rural communities builds response capacity at the grassroots level, and has improved dialogue between local stakeholders responsible for monitoring human and animal health. Here we describe the process of initiating participatory research and cohort field implementation over the first year in two study sites in Vietnam. Keywords: Vietnam, participatory research, zoonoses, field epidemiology
- Published
- 2015
- Full Text
- View/download PDF
4. The Vietnam Initiative on Zoonotic Infections (VIZIONS): A Strategic Approach to Studying Emerging Zoonotic Infectious Diseases
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Rabaa, Maia A., Tue, Ngo Tri, Phuc, Tran My, Carrique-Mas, Juan, Saylors, Karen, Cotten, Matthew, Bryant, Juliet E., Nghia, Ho Dang Trung, Cuong, Nguyen Van, Pham, Hong Anh, Berto, Alessandra, Phat, Voong Vinh, Dung, Tran Thi Ngoc, Bao, Long Hoang, Hoa, Ngo Thi, Wertheim, Heiman, Nadjm, Behzad, Monagin, Corina, van Doorn, H. Rogier, Rahman, Motiur, Tra, My Phan Vu, Campbell, James I., Boni, Maciej F., Tam, Pham Thi Thanh, van der Hoek, Lia, Simmonds, Peter, Rambaut, Andrew, Toan, Tran Khanh, Van Vinh Chau, Nguyen, Hien, Tran Tinh, Wolfe, Nathan, Farrar, Jeremy J., Thwaites, Guy, Kellam, Paul, Woolhouse, Mark E. J., and Baker, Stephen
- Published
- 2015
- Full Text
- View/download PDF
5. Mobilising community-based research on zoonotic infections: A case study of longitudinal cohorts in Vietnam
- Author
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Hoa Ngo Thi, Stephen Baker, Heiman F. L. Wertheim, Kiet Bach Tuan, Toan Tran Khanh, Karen Saylors, Tue Ngo Tri, and Juliet E. Bryant
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Disease surveillance ,medicine.medical_specialty ,business.industry ,Public health ,Vietnamese ,Participatory action research ,lcsh:Human settlements. Communities ,lcsh:HT51-65 ,General Medicine ,language.human_language ,Grassroots ,One Health ,Environmental health ,Epidemiology ,language ,Medicine ,business ,Cohort study - Abstract
We initiated the Vietnam Initiative on Zoonotic Infections (VIZIONS) research program to better understand the epidemiology of disease transmission at the human–animal interface in Vietnamese rural communities and to integrate One Health approaches to disease surveillance. We established a longitudinal community cohort study of individuals with occupational exposure to animals, which involves concurrent targeted sampling from domestic livestock species and follow-up monitoring of human clinical cases. The project was implemented by government agencies at the provincial and district levels of the public health and animal health sectors in Vietnam. Engaging with rural communities builds response capacity at the grassroots level, and has improved dialogue between local stakeholders responsible for monitoring human and animal health. Here we describe the process of initiating participatory research and cohort field implementation over the first year in two study sites in Vietnam.Keywords: Vietnam, participatory research, zoonoses, field epidemiology
- Published
- 2015
6. New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
- Author
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Mather, Alison E., primary, Phuong, Tu Le Thi, additional, Gao, Yunfeng, additional, Clare, Simon, additional, Mukhopadhyay, Subhankar, additional, Goulding, David A., additional, Hoang, Nhu Tran Do, additional, Tuyen, Ha Thanh, additional, Lan, Nguyen Phu Huong, additional, Thompson, Corinne N., additional, Trang, Nguyen Hoang Thu, additional, Carrique-Mas, Juan, additional, Tue, Ngo Tri, additional, Campbell, James I., additional, Rabaa, Maia A., additional, Thanh, Duy Pham, additional, Harcourt, Katherine, additional, Hoa, Ngo Thi, additional, Trung, Nguyen Vinh, additional, Schultsz, Constance, additional, Perron, Gabriel G., additional, Coia, John E., additional, Brown, Derek J., additional, Okoro, Chinyere, additional, Parkhill, Julian, additional, Thomson, Nicholas R., additional, Chau, Nguyen Van Vinh, additional, Thwaites, Guy E., additional, Maskell, Duncan J., additional, Dougan, Gordon, additional, Kenney, Linda J., additional, and Baker, Stephen, additional
- Published
- 2018
- Full Text
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7. Unbiased whole-genome deep sequencing of human and porcine stool samples reveals circulation of multiple groups of rotaviruses and a putative zoonotic infection
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Juliet E. Bryant, Maia A. Rabaa, Phuc Tran My, Guy E. Thwaites, Paul Kellam, Nguyen Van Cuong, Mark E. J. Woolhouse, My V. T. Phan, Tue Ngo Tri, Lia van der Hoek, Bas B. Oude Munnink, Pham Hong Anh, Stephen Baker, Matthew Cotten, Wellcome Trust, Medical Microbiology and Infection Prevention, and Amsterdam institute for Infection and Immunity
- Subjects
VIETNAM ,0301 basic medicine ,virus surveillance ,UNITED-STATES ,VACCINE ,CHILDREN ,Genomics ,zoonotic infection ,Biology ,medicine.disease_cause ,Microbiology ,Group A ,Genome ,Deep sequencing ,Virus ,03 medical and health sciences ,deep sequencing ,whole genomes ,ACUTE GASTROENTERITIS ,Virology ,Rotavirus ,Genotype ,medicine ,on behalf of the VIZIONS Consortium ,030304 developmental biology ,Genetics ,0303 health sciences ,Science & Technology ,IDENTIFICATION ,Phylogenetic tree ,Zoonotic Infection ,030306 microbiology ,STRAINS ,BOVINE ROTAVIRUS ,DIARRHEA ,3. Good health ,030104 developmental biology ,rotavirus ,VIZIONS Consortium ,GROUP-A ROTAVIRUSES ,Life Sciences & Biomedicine ,Research Article - Abstract
Coordinated and synchronous virological surveillance for zoonotic viruses in both human clinical cases and animal reservoirs provides an opportunity to identify interspecies virus movement. Rotavirus is an important cause of viral gastroenteritis in humans and animals. We have documented the rotavirus diversity within co-located humans and animals sampled from the Mekong delta region of Vietnam using a primer-independent, agnostic, deep sequencing approach. A total of 296 stool samples (146 from diarrhoeal human patients and 150 from pigs living in the same geographical region) were directly sequenced, generating the genomic sequences of 60 human rotaviruses (all group A) and 31 porcine rotaviruses (13 group A, 7 group B, 6 group C and 5 group H). Phylogenetic analyses showed the co-circulation of multiple distinct rotavirus group A (RVA) genotypes/strains, many of which were divergent from the strain components of licensed RVA vaccines, as well as considerable virus diversity in pigs including full genomes of rotaviruses in groups B, C and H, none of which have been previously reported in Vietnam. Furthermore the detection of an atypical RVA genotype constellation (G4-P[6]-I1-R1-C1-M1-A8-N1-T7-E1-H1) in a human patient and a pig from the same region provides some evidence for a zoonotic event
- Published
- 2016
8. Occupational Animal Contact in Southern and Central Vietnam.
- Author
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Tu, Nguyen Thi Kha, Tue, Ngo Tri, Vapalahti, Olli, Virtala, Anna-Maija K., Van Tan, Le, Rabaa, Maia A., Carrique-Mas, Juan, Thwaites, Guy E., and Baker, Stephen
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ANIMAL health ,ZOONOSES ,EXOTIC animals ,PROTECTIVE clothing ,WORK-related injuries ,EMERGING infectious diseases ,LIVESTOCK - Abstract
Despite the global zoonotic disease burden, the underlying exposures that drive zoonotic disease emergence are not understood. Here, we aimed to assess exposures to potential sources of zoonotic disease and investigate the demographics, attitudes, and behavior of individuals with sustained occupational animal contact in Vietnam. We recruited 581 animal workers (animal-raising farmers, slaughterers, animal health workers, and rat traders) and their families in southern and central Vietnam into a cohort. Cohort members were followed for 3 years and interviewed annually regarding (1) demography and attitudes regarding zoonotic disease, (2) medical history, (3) specific exposures to potential zoonotic infection sources, and (4) socioeconomic status. Interview information over the 3 years was combined and analyzed as cross-sectional data. Of the 297 cohort members interviewed, the majority (79.8%; 237/297) reported raising livestock; almost all (99.6%; 236/237) reported being routinely exposed to domestic animals, and more than a quarter (28.7%; 68/237) were exposed to exotic animals. Overall, 70% (208/297) reported slaughtering exotic animals; almost all (99.5%; 207/208) reported consuming such animals. The consumption of raw blood and meat was common (24.6%; 73/297 and 37%; 110/297, respectively). Over half (58.6%; 174/297) reported recent occupational animal-induced injuries that caused bleeding; the use of personal protective equipment (PPE) was limited. Our work demonstrates that individuals working with animals in Vietnam are exposed to a wide range of species, and there are limited procedures for reducing potential zoonotic disease exposures. We advocate better education, improved animal security, and enforced legislation of PPE for those with occupational animal exposure in Vietnam. [ABSTRACT FROM AUTHOR]
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- 2019
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9. Diversity of Bartonella spp. in bats, southern Vietnam
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Anh, Pham Hong, Van Cuong, Nguyen, Son, Nguyen Truong, Tue, Ngo Tri, Kosoy, Michael, Woolhouse, Mark E.J., Baker, Stephen, Bryant, Juliet E., Thwaites, Guy, Carrique-Mas, Juan J., and Rabaa, Maia A.
- Subjects
Bats (Animals) -- Physiological aspects ,Bartonella -- Distribution ,Host-parasite relationships -- Research ,Medical research ,Health - Abstract
To the Editor: To investigate bats as potential reservoirs for Bartonella spp. in Vietnam, we screened a range of bat species to determine the prevalence and genetic diversity of Bartonella [...]
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- 2015
10. Diversity ofBartonellaspp. in Bats, Southern Vietnam
- Author
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Anh, Pham Hong, primary, Van Cuong, Nguyen, additional, Son, Nguyen Truong, additional, Tue, Ngo Tri, additional, Kosoy, Michael, additional, Woolhouse, Mark E.J., additional, Baker, Stephen, additional, Bryant, Juliet E., additional, Thwaites, Guy, additional, Carrique-Mas, Juan J., additional, and Rabaa, Maia A., additional
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- 2015
- Full Text
- View/download PDF
11. Rodents and Risk in the Mekong Delta of Vietnam: Seroprevalence of Selected Zoonotic Viruses in Rodents and Humans
- Author
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Van Cuong, Nguyen, primary, Carrique-Mas, Juan, additional, Vo Be, Hien, additional, An, Nguyen Ngoc, additional, Tue, Ngo Tri, additional, Anh, Nguyet Lam, additional, Anh, Pham Hong, additional, Phuc, Nguyen The, additional, Baker, Stephen, additional, Voutilainen, Liina, additional, Jääskeläinen, Anne, additional, Huhtamo, Eili, additional, Utriainen, Mira, additional, Sironen, Tarja, additional, Vaheri, Antti, additional, Henttonen, Heikki, additional, Vapalahti, Olli, additional, Chaval, Yannick, additional, Morand, Serge, additional, and Bryant, Juliet E., additional
- Published
- 2015
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- View/download PDF
12. Mobilising Community-Based Research on Zoonotic Infections.
- Author
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Saylors, Karen, Tue Ngo Tri, Toan Tran Khanh, Kiet Bach Tuan, Wertheim, Heiman F. L., Baker, Stephen, Hoa Ngo Thi, and Bryant, Juliet E.
- Subjects
- *
ZOONOSES , *COMMUNICABLE diseases , *DISEASE vectors , *MEDICAL zoology - Abstract
The article examines how the Vietnam Initiative on Zoonotic Infections (VIZIONS) community cohort program conducts integrated management of human-animal infectious disease control through the One Health research agenda of the World Health Organization (WHO). Topics include the structure of the program, the cohort's protocol, and methods and implementation approach of the program in two sites. Also mentioned is how VIZION galvanize other communities to inform its extension to other areas.
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- 2015
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13. Sample descriptors linked to metagenomic sequencing data from human and animal enteric samples from Vietnam
- Author
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Woolhouse, Mark, Ashworth, Jordan, Bogaardt, Carlijn, Tue, Ngo Tri, Baker, Steve, Thwaites, Guy, and Phuc, Tran My
- Subjects
Mammals ,Metadata ,Vietnam ,Swine ,Viruses ,Animals ,Humans ,Metagenome ,Rodentia ,3. Good health - Abstract
There is still limited information on the diversity of viruses co-circulating in humans and animals. Here, we report data obtained from a large field collection of enteric samples taken from humans, pigs, rodents and other mammal hosts in Vietnam between 2012 and 2016. Each of 2100 stool or rectal swab samples was subjected to virally-enriched agnostic metagenomic sequencing; the short read sequence data are accessible from the European Nucleotide Archive (ENA). We link the sequence data to metadata on host type and demography and geographic location, distinguishing hospital patients, members of a cohort identified as a high risk of zoonotic infections (e.g. abattoir workers, rat traders) and animals. These data are suitable for further studies of virus diversity and virus discovery in humans and animals from Vietnam and to identify viruses found in multiple hosts that are potentially zoonotic.
14. Occupational Animal Contact in Southern and Central Vietnam
- Author
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Tu, Nguyen Thi Kha, Tue, Ngo Tri, Vapalahti, Olli, Virtala, Anna-Maija K, Van Tan, Le, Rabaa, Maia A, Carrique-Mas, Juan, Thwaites, Guy E, Baker, Stephen, and VIZIONS Consortium
- Subjects
Adult ,Male ,Adolescent ,Exposure risk ,Risk Assessment ,Zoonosis ,Young Adult ,Occupational Exposure ,Surveys and Questionnaires ,Zoonoses ,Animals ,Humans ,Animal health workers ,Aged ,2. Zero hunger ,Aged, 80 and over ,Farmers ,Slaughterers ,Cohort ,Middle Aged ,3. Good health ,Emerging infections ,Meat Products ,Cross-Sectional Studies ,Vietnam ,Socioeconomic Factors ,Female ,Abattoirs - Abstract
Despite the global zoonotic disease burden, the underlying exposures that drive zoonotic disease emergence are not understood. Here, we aimed to assess exposures to potential sources of zoonotic disease and investigate the demographics, attitudes, and behavior of individuals with sustained occupational animal contact in Vietnam. We recruited 581 animal workers (animal-raising farmers, slaughterers, animal health workers, and rat traders) and their families in southern and central Vietnam into a cohort. Cohort members were followed for 3 years and interviewed annually regarding (1) demography and attitudes regarding zoonotic disease, (2) medical history, (3) specific exposures to potential zoonotic infection sources, and (4) socioeconomic status. Interview information over the 3 years was combined and analyzed as cross-sectional data. Of the 297 cohort members interviewed, the majority (79.8%; 237/297) reported raising livestock; almost all (99.6%; 236/237) reported being routinely exposed to domestic animals, and more than a quarter (28.7%; 68/237) were exposed to exotic animals. Overall, 70% (208/297) reported slaughtering exotic animals; almost all (99.5%; 207/208) reported consuming such animals. The consumption of raw blood and meat was common (24.6%; 73/297 and 37%; 110/297, respectively). Over half (58.6%; 174/297) reported recent occupational animal-induced injuries that caused bleeding; the use of personal protective equipment (PPE) was limited. Our work demonstrates that individuals working with animals in Vietnam are exposed to a wide range of species, and there are limited procedures for reducing potential zoonotic disease exposures. We advocate better education, improved animal security, and enforced legislation of PPE for those with occupational animal exposure in Vietnam.
15. New Variant of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
- Author
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Mather, Alison E, Phuong, Tu Le Thi, Gao, Yunfeng, Clare, Simon, Mukhopadhyay, Subhankar, Goulding, David A, Hoang, Nhu Tran Do, Tuyen, Ha Thanh, Lan, Nguyen Phu Huong, Thompson, Corinne N, Trang, Nguyen Hoang Thu, Carrique-Mas, Juan, Tue, Ngo Tri, Campbell, James I, Rabaa, Maia A, Thanh, Duy Pham, Harcourt, Katherine, Hoa, Ngo Thi, Trung, Nguyen Vinh, Schultsz, Constance, Perron, Gabriel G, Coia, John E, Brown, Derek J, Okoro, Chinyere, Parkhill, Julian, Thomson, Nicholas R, Chau, Nguyen Van Vinh, Thwaites, Guy E, Maskell, Duncan J, Dougan, Gordon, Kenney, Linda J, and Baker, Stephen
- Subjects
Salmonella typhimurium ,Genotype ,Swine ,Bacteremia ,HIV Infections ,Immunocompromised Host ,Drug Resistance, Multiple, Bacterial ,Zoonoses ,genomics ,Disease Transmission, Infectious ,Animals ,Humans ,antimicrobial resistance ,Molecular Epidemiology ,Salmonella Infections, Animal ,Whole Genome Sequencing ,Genetic Variation ,3. Good health ,invasive salmonellosis ,Gastroenteritis ,Ducks ,Vietnam ,Carrier State ,Salmonella Infections ,bacteria ,Chickens - Abstract
Nontyphoidal Salmonella (NTS), particularly Salmonella enterica serovar Typhimurium, is among the leading etiologic agents of bacterial enterocolitis globally and a well-characterized cause of invasive disease (iNTS) in sub-Saharan Africa. In contrast, S Typhimurium is poorly defined in Southeast Asia, a known hot spot for zoonotic disease with a recently described burden of iNTS disease. Here, we aimed to add insight into the epidemiology and potential impact of zoonotic transfer and antimicrobial resistance (AMR) in S Typhimurium associated with iNTS and enterocolitis in Vietnam. We performed whole-genome sequencing and phylogenetic reconstruction on 85 human (enterocolitis, carriage, and iNTS) and 113 animal S Typhimurium isolates isolated in Vietnam. We found limited evidence for the zoonotic transmission of S Typhimurium. However, we describe a chain of events where a pandemic monophasic variant of S Typhimurium (serovar I:4,[5],12:i:- sequence type 34 [ST34]) has been introduced into Vietnam, reacquired a phase 2 flagellum, and acquired an IncHI2 multidrug-resistant plasmid. Notably, these novel biphasic ST34 S Typhimurium variants were significantly associated with iNTS in Vietnamese HIV-infected patients. Our study represents the first characterization of novel iNTS organisms isolated outside sub-Saharan Africa and outlines a new pathway for the emergence of alternative Salmonella variants into susceptible human populations.IMPORTANCESalmonella Typhimurium is a major diarrheal pathogen and associated with invasive nontyphoid Salmonella (iNTS) disease in vulnerable populations. We present the first characterization of iNTS organisms in Southeast Asia and describe a different evolutionary trajectory from that of organisms causing iNTS in sub-Saharan Africa. In Vietnam, the globally distributed monophasic variant of Salmonella Typhimurium, the serovar I:4,[5],12:i:- ST34 clone, has reacquired a phase 2 flagellum and gained a multidrug-resistant plasmid to become associated with iNTS disease in HIV-infected patients. We document distinct communities of S Typhimurium and I:4,[5],12:i:- in animals and humans in Vietnam, despite the greater mixing of these host populations here. These data highlight the importance of whole-genome sequencing surveillance in a One Health context in understanding the evolution and spread of resistant bacterial infections.
16. Sample descriptors linked to metagenomic sequencing data from human and animal enteric samples from Vietnam
- Author
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Woolhouse, Mark, Ashworth, Jordan, Bogaardt, Carlijn, Tue, Ngo Tri, Baker, Steve, Thwaites, Guy, and Phuc, Tran My
- Subjects
Data Descriptor ,692/699/255/2514 ,631/208/514/2254 ,data-descriptor ,3. Good health - Abstract
There is still limited information on the diversity of viruses co-circulating in humans and animals. Here, we report data obtained from a large field collection of enteric samples taken from humans, pigs, rodents and other mammal hosts in Vietnam between 2012 and 2016. Each of 2100 stool or rectal swab samples was subjected to virally-enriched agnostic metagenomic sequencing; the short read sequence data are accessible from the European Nucleotide Archive (ENA). We link the sequence data to metadata on host type and demography and geographic location, distinguishing hospital patients, members of a cohort identified as a high risk of zoonotic infections (e.g. abattoir workers, rat traders) and animals. These data are suitable for further studies of virus diversity and virus discovery in humans and animals from Vietnam and to identify viruses found in multiple hosts that are potentially zoonotic.
17. New Variant of Multidrug-Resistant Salmonella entericaSerovar Typhimurium Associated with Invasive Disease in Immunocompromised Patients in Vietnam
- Author
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Mather, Alison E., Phuong, Tu Le Thi, Gao, Yunfeng, Clare, Simon, Mukhopadhyay, Subhankar, Goulding, David A., Hoang, Nhu Tran Do, Tuyen, Ha Thanh, Lan, Nguyen Phu Huong, Thompson, Corinne N., Trang, Nguyen Hoang Thu, Carrique-Mas, Juan, Tue, Ngo Tri, Campbell, James I., Rabaa, Maia A., Thanh, Duy Pham, Harcourt, Katherine, Hoa, Ngo Thi, Trung, Nguyen Vinh, Schultsz, Constance, Perron, Gabriel G., Coia, John E., Brown, Derek J., Okoro, Chinyere, Parkhill, Julian, Thomson, Nicholas R., Chau, Nguyen Van Vinh, Thwaites, Guy E., Maskell, Duncan J., Dougan, Gordon, Kenney, Linda J., and Baker, Stephen
- Abstract
SalmonellaTyphimurium is a major diarrheal pathogen and associated with invasive nontyphoid Salmonella(iNTS) disease in vulnerable populations. We present the first characterization of iNTS organisms in Southeast Asia and describe a different evolutionary trajectory from that of organisms causing iNTS in sub-Saharan Africa. In Vietnam, the globally distributed monophasic variant of SalmonellaTyphimurium, the serovar I:4,[5],12:i:− ST34 clone, has reacquired a phase 2 flagellum and gained a multidrug-resistant plasmid to become associated with iNTS disease in HIV-infected patients. We document distinct communities of S. Typhimurium and I:4,[5],12:i:− in animals and humans in Vietnam, despite the greater mixing of these host populations here. These data highlight the importance of whole-genome sequencing surveillance in a One Health context in understanding the evolution and spread of resistant bacterial infections.
- Published
- 2018
- Full Text
- View/download PDF
18. Genetic diversity and cross-species transmission of kobuviruses in Vietnam.
- Author
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Lu L, Van Dung N, Ivens A, Bogaardt C, O'Toole A, Bryant JE, Carrique-Mas J, Van Cuong N, Anh PH, Rabaa MA, Tue NT, Thwaites GE, Baker S, Simmonds P, and Woolhouse ME
- Abstract
Cross-species transmission of viruses poses a sustained threat to public health. Due to increased contact between humans and other animal species the possibility exists for cross-species transmissions and ensuing disease outbreaks. By using conventional PCR amplification and next generation sequencing, we obtained 130 partial or full genome kobuvirus sequences from humans in a sentinel cohort in Vietnam and various mammalian hosts including bats, rodents, pigs, cats, and civets. The evolution of kobuviruses in different hosts was analysed using Bayesian phylogenetic methods. We estimated and compared time of origin of kobuviruses in different host orders; we also examined the cross-species transmission of kobuviruses within the same host order and between different host orders. Our data provide new knowledge of rodent and bat kobuviruses, which are most closely related to human kobuviruses. The novel bat kobuviruses isolated from bat roosts in Southern Vietnam were genetically distinct from previously described bat kobuviruses, but closely related to kobuviruses found in rodents. We additionally found evidence of frequent cross-species transmissions of kobuviruses within rodents. Overall, our phylogenetic analyses reveal multiple cross-species transmissions both within and among mammalian species, which increases our understanding of kobuviruses genetic diversity and the complexity of their evolutionary history.
- Published
- 2018
- Full Text
- View/download PDF
19. Rodents and risk in the Mekong Delta of Vietnam: seroprevalence of selected zoonotic viruses in rodents and humans.
- Author
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Van Cuong N, Carrique-Mas J, Vo Be H, An NN, Tue NT, Anh NL, Anh PH, Phuc NT, Baker S, Voutilainen L, Jääskeläinen A, Huhtamo E, Utriainen M, Sironen T, Vaheri A, Henttonen H, Vapalahti O, Chaval Y, Morand S, and Bryant JE
- Subjects
- Animals, Arenavirus immunology, Arenavirus isolation & purification, Cowpox virus immunology, Cowpox virus isolation & purification, Flavivirus immunology, Flavivirus isolation & purification, Orthohantavirus immunology, Orthohantavirus isolation & purification, Humans, Parechovirus immunology, Parechovirus isolation & purification, Rodentia immunology, Vietnam epidemiology, Zoonoses, Disease Vectors, Meat virology, Rodentia virology, Seroepidemiologic Studies
- Abstract
In the Mekong Delta in southern Vietnam, rats are commonly traded in wet markets and sold live for food consumption. We investigated seroprevalence to selected groups of rodent-borne viruses among human populations with high levels of animal exposure and among co-located rodent populations. The indirect fluorescence antibody test (IFAT) was used to determine seropositivity to representative reference strains of hantaviruses (Dobrava virus [DOBV], Seoul virus [SEOV]), cowpox virus, arenaviruses (lymphocytic choriomeningitis virus [LCMV]), flaviviruses (tick-borne encephalitis virus [TBEV]), and rodent parechoviruses (Ljungan virus), using sera from 245 humans living in Dong Thap Province and 275 rodents representing the five common rodent species sold in wet markets and present in peridomestic and farm settings. Combined seropositivity to DOBV and SEOV among the rodents and humans was 6.9% (19/275) and 3.7% (9/245), respectively; 1.1% (3/275) and 4.5% (11/245) to cowpox virus; 5.4% (15/275) and 47.3% (116/245) for TBEV; and exposure to Ljungan virus was 18.8% (46/245) in humans, but 0% in rodents. Very little seroreactivity was observed to LCMV in either rodents (1/275, 0.4%) or humans (2/245, 0.8%). Molecular screening of rodent liver tissues using consensus primers for flaviviruses did not yield any amplicons, whereas molecular screening of rodent lung tissues for hantavirus yielded one hantavirus sequence (SEOV). In summary, these results indicate low to moderate levels of endemic hantavirus circulation, possible circulation of a flavivirus in rodent reservoirs, and the first available data on human exposures to parechoviruses in Vietnam. Although the current evidence suggests only limited exposure of humans to known rodent-borne diseases, further research is warranted to assess public health implications of the rodent trade.
- Published
- 2015
- Full Text
- View/download PDF
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