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3 results on '"Turos L"'

1. Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells

Author

Daniel M. Davis, Kolba, Katja Srpan, Dominik Cysewski, Yannick Schwab, Wioleta Dudka, Katarzyna Piwocka, Turos L, Monika Zaręba-Kozioł, Paolo Ronchi, Agata Kominek, and Jakub Wlodarczyk

Subjects
Stromal cell, Chemistry, Vesicle, medicine.disease, Cytoprotection, law.invention, Cell biology, Leukemia, medicine.anatomical_structure, Stroma, Confocal microscopy, law, medicine, Bone marrow, Intracellular
Abstract

Intercellular communication within the bone marrow niche significantly influences leukemogenesis and the sensitivity of leukemic cells to therapy. Tunneling nanotubes (TNTs) are a novel mode of intercellular cross-talk. They are long, thin membranous protrusions that enable the direct transfer of various cargo between cells. Here we show that TNTs are formed between leukemic and bone marrow stromal cells. Fluorescence confocal microscopy with 3D reconstructions, correlative light-electron microscopy and electron tomography provided evidence that TNTs transfer cellular vesicles between cells. The quantitative analysis demonstrated that the stromal cells stimulate TNT-mediated vesicle transfer towards leukemic cells. Transfer of vesicular cargo from stromal cells correlated with increased resistance to anti-leukemic treatment. Moreover, specific sets of proteins with a potential role in survival and the drug response were transferred within these vesicles. Altogether, we found that TNTs are involved in the leukemia-stroma cross-talk and the stroma-mediated cytoprotection of leukemic cells. Our findings implicate TNT connections as a possible target for therapeutic interventions within the leukemia microenvironment to attenuate stroma-conferred protection.

Published
2018
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2. SEPIC Based Solar Array Simulator.

Author

Szekely, I., Turos, L., Hegyi, I., and Csernáth, G.

Subjects
SOLAR cells, PHOTOVOLTAIC cells, CONVERTERS (Electronics), ELECTRIC circuit networks
Abstract

A laboratory grade electrical photovoltaic simulator system is presented in the paper offering a viable alternative for simulating direct sunlight effects in indoor experiments. In order to validate the photovoltaic model for a single cell, the simulated characteristics were compared against the selected cell's datasheet. The SEPIC converter was chosen for its characteristics that it can cover a wide range of output voltage, including its good behavior near short circuit conditions. The SEPIC Simulink model was developed and validated by PSPICE simulation. Using the SEPIC transfer characteristics the achievable resolution has been verified and the tracking algorithm has been detailed which follows the I-V curves of the simulated solar array in different load conditions. [ABSTRACT FROM AUTHOR]

Published
2014

3. Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells.

Author

Kolba MD, Dudka W, Zaręba-Kozioł M, Kominek A, Ronchi P, Turos L, Chroscicki P, Wlodarczyk J, Schwab Y, Klejman A, Cysewski D, Srpan K, Davis DM, and Piwocka K

Subjects
Biological Transport genetics, Cell Communication drug effects, Cell Line, Tumor, Drug Resistance, Neoplasm genetics, Humans, Imatinib Mesylate chemistry, Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics, Leukemia, Myelogenous, Chronic, BCR-ABL Positive pathology, Microscopy, Electron, Mitochondria drug effects, Stromal Cells drug effects, Stromal Cells ultrastructure, Imatinib Mesylate pharmacology, Leukemia, Myelogenous, Chronic, BCR-ABL Positive drug therapy, Mitochondria genetics, Nanotubes chemistry
Abstract

Intercellular communication within the bone marrow niche significantly promotes leukemogenesis and provides protection of leukemic cells from therapy. Secreted factors, intercellular transfer of mitochondria and the receptor-ligand interactions have been shown as mediators of this protection. Here we report that tunneling nanotubes (TNTs)-long, thin membranous structures, which have been identified as a novel mode of intercellular cross-talk-are formed in the presence of stroma and mediate transfer of cellular vesicles from stroma to leukemic cells. Importantly, transmission of vesicles via TNTs from stromal cells increases resistance of leukemic cells to the tyrosine kinase inhibitor, imatinib. Using correlative light-electron microscopy and electron tomography we show that stromal TNTs contain vesicles, provide membrane continuity with the cell bodies and can be open-ended. Moreover, trans-SILAC studies to reveal the non-autonomous proteome showed that specific sets of proteins are transferred together with cellular vesicles from stromal to leukemic cells, with a potential role in survival and adaptation. Altogether, our findings provide evidence for the biological role of the TNT-mediated vesicle exchange between stromal and leukemic cells, implicating the direct vesicle and protein transfer in the stroma-provided protection of leukemic cells.

Published
2019
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