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Aims and objectives/purpose/research questions: Prior research has put forward that asymmetrical switch costs in language switching do not occur when bilinguals are in a voluntary language-switching context. While several studies have provided evidence along these lines, two very recent studies have put this into question. This study further investigates the possibility that voluntary language switching abolishes asymmetrical switch costs. Design/methodology/approach: We presented German-English bilinguals (n = 79) with pictures that had to be named in either German or English, whichever they chose, by typing. Data and analysis: Reaction times (both the latency of the first keystroke and interkeystroke intervals) were analyzed using linear mixed-effects regression modeling with the factors Trial type (switch vs. repetition trial) and Language (German vs. English), whereas the error rates were analyzed using logistic mixed-effects regression modeling. Findings/conclusions: The onset latencies and inter-keystroke intervals both showed larger second-language (English) than first-language (German) switch costs (i.e., reversed asymmetrical switch costs). As switch costs were modulated by language dominance in a voluntary language-switching context, this study provides evidence that voluntary language switching is not a boundary condition for asymmetrical switch costs. Originality: In contrast to most previous language-switching studies, we used typed rather than vocal responses in a voluntary language-switching paradigm. In addition, our findings further support and generalize very recent findings of (reversed) asymmetrical switch costs in voluntary language switching. Significance/implications: Since asymmetrical switch costs, an important measure of bilingual inhibitory control, are not consistently observed throughout the language-switching literature, it is essential to understand its boundary conditions. [ABSTRACT FROM AUTHOR]

Error detection in typing is crucial for assessing the adequacy of ongoing actions, leveraging both predictive mechanisms for early detection and sensory feedback for late detection. Neurophysiological studies have supported the anticipation of errors through predictive models. This research extends the understanding of error detection in typing, focusing on the neurocognitive mechanisms underlying errors in transparent and intransparent German words. Thirty-six volunteer students typed out aurally presented words, classified as either orthographically transparent or intransparent, on a computer keyboard without the possibility of correction. Because of poor spelling or excessive artifacts, the final sample comprised 27 participants. Event-related potentials (ERPs) were obtained time-locked to key presses, and behavioral data on typing correctness and speed were collected. A higher error rate and longer latency for intransparent words compared to transparent ones were found, suggesting the complexity of spelling impacts typing correctness. Post-error slowing was observed, aligning with increased cognitive control following errors. ERPs revealed a negative component akin to the error-related negativity (ERN) for typing errors, with a pronounced ERN-like negativity preceding erroneous key-presses, particularly for intransparent words. The study provides evidence of the cognitive and neural mechanisms underlying typing errors, highlighting the impact of orthographic transparency. The detection of an ERN-like negativity before erroneous key-presses, especially in typing intransparent words, underscores the brain's use of predictive mechanisms for error detection. • Typing errors occur more frequently with intransparent than transparent words. • Post-error slowing indicates a rise in cognitive control. • ERN-like negativity shows predictive error detection in the brain. • Sensory feedback is not crucial for detecting typing errors. [ABSTRACT FROM AUTHOR]

The keyboard, in a physical or virtual form, is one of the most popular and available communication mediums for computers and mobile devices. In this paper, we introduce the Keydrop, a novel virtual keyboard layout for a touchscreen that dynamically changes the keys' labels. The results collected from 54 participants showed that Keydrop outperforms the usual QWERTY virtual keyboard in terms of typing speed and the number of typos. The solution gained positive feedback from participants, and the empirical results showed that users type 6% faster, making 33% fewer typos. The proposed solution achieves a speed of 25.17 words per minute (WPM) and 1.20 keystrokes per character (KSPC). [ABSTRACT FROM AUTHOR]

Streptococcus pyogenes (group A Streptococcus, GAS) is a major human pathogen and causes every year over 600 millions upper respiratory tract onfections worldwide. Untreated or repeated infections may lead to post-infectional sequelae such as rheumatic heart disease, a major cause of GAS-mediated mortality. There is no comprehensive, longitudinal analysis of the M type distribution of upper respiratory tract strains isolated in Poland. Single reports describe rather their antibiotic resistance patterns or focus on the invasive isolates. Our goal was to analyse the clonal structure of the upper respiratory tract GAS isolated over multiple years in Poland. Our analysis revealed a clonal structure similar to the ones observed in high-income countries, with M1, M12, M89, M28, and M77 serotypes constituting over 80% of GAS strains. The M77 serotype is a major carrier of erythromycin resistance and is more often correlated with upper respiratory tract infections than other serotypes. [ABSTRACT FROM AUTHOR]

Enterovirus (EV) infections have various symptoms and severe complications, including death. To determine EV prevalence and EV types in Slovenia, data on over 25 000 EV RNA tests for diagnostics and surveillance from 2014 to 2023 were analyzed. Altogether, 3733 cerebrospinal fluid (CSF) and 21 297 respiratory (sentinel and clinical) samples were tested for EV RNA. EV typing was performed on all residual EV‐positive CSF samples and on subset of respiratory specimens. Altogether, 1238 samples tested positive for EV RNA: 238 (6.4%) CSF and 1000 (4.7%) respiratory samples. EV‐positive patients were predominantly male (p < 0.001). Many EV‐positive CSF samples were from infants under 3 months (33.1%), whereas most EV‐positive respiratory samples were from children 1 to 2 years old (49.2%). Echovirus 30 (E‐30) was most frequent in CSF (33.0%), followed by CV‐B5 (13.8%) and E‐6 (13.8%). CV‐A6 was most frequent in respiratory samples (16.0%), followed by EV‐D68 (7.6%) and CV‐A5 (7.4%). EV types in CSF and respiratory samples show diverse dynamics, with some outbreaks indicated. A significant difference was found in the EV detection rate between CSF and respiratory samples by age. Various EV types were characterized, showing that some EV types are more neurotropic or cause more severe infections. [ABSTRACT FROM AUTHOR]

Low temperature, rainfall/snowfall and freezing is a complex climate event. Although extreme low temperature, rainfall/snowfall and freezing events occur infrequently, they often cause serious impacts. Researching evolutionary characteristics of low temperature, rainfall/ snowfall and freezing events is of great significance for effective defense and mitigation of disaster impacts. By using daily meteorological observation data from 1981 to 2024 in Hubei and selecting indicators from three aspects: low temperature, rainfall/snowfall and freezing, a comprehensive evaluation model for large-scale sustained low temperature, rainfall/snowfall and freezing events was established. According to the types of disaster-causing factors and the disaster characteristics of the affected bodies, these events were classified and ranked. Combined with the historical disaster data, the disaster characteristics of large-scale sustained low temperature, rainfall/snowfall and freezing events in Hubei since 1981 were then analyzed. The results are as follows. (1) There have been a total of 64 large-scale sustained low temperature, rainfall/snowfall and freezing events in Hubei in the past 44 years, with an average of 1.5 occurrences per year. January has the highest frequency of occurrence, accounting for about 42%, followed by February, accounting for about 34%. (2) The large-scale sustained low temperature, rainfall/snowfall and freezing events in Hubei are classified into six types, with rain and snow type accounting for 40%, low temperature, rainfall/snowfall and freezing type accounting for 31%, and low temperature, rain, and snow type accounting for 16%, other types accounting for a total of 13%. The strongest 10 events all belong to low temperature, rainfall/snowfall and freezing type. (3) The direct economic losses caused by low temperature, rainfall/snowfall and freezing disasters in Hubei have shown an overall increasing trend, but the proportion of direct economic losses to GDP has shown a certain decreasing trend, with a relatively small proportion since 2012. (4) The correlation between the comprehensive intensity index and direct economic losses of large-scale sustained low temperature, rainfall/snowfall and freezing events in Hubei follows a power curve distribution. When the comprehensive intensity index of the event exceeds 1.85, the direct economic losses increase rapidly.

BACKGROUND: Talus cartilage injury is a common motor system disease. This type of injury will affect the patient’s daily life and work ability, and may worsen the condition if left untreated. Surgical treatment is commonly used, but the selection of surgical methods and the evaluation of medium- and long-term follow-up results have always been difficult clinical problems. OBJECTIVE: To explore the influence of T1ρ technique on the range of quantitative evaluation of talus osteochondral injury on the choice of surgical method and the results of medium- and long-term follow-up. METHODS: A total of 154 patients with osteochondral injury of talus admitted to The Second Hospital of Tangshan from January 2019 to August 2022 were retrospectively selected as the study subjects. The lesion site of talus was examined by MRI before operation, and the T1ρ and T2 values of different types were compared. Different surgical methods were selected according to the different T1ρ values. Group A (n=73) was treated with microfracture surgery with T1ρ < 45 ms; group B (n=81) was treated with autogenous bone and cartilage transplantation with T1ρ ≥ 45 ms. The general clinical characteristics and curative effects of patients under different surgical methods were compared; the important factors of postoperative recurrence were analyzed by multivariate Logistic regression, and the relationship between T1ρ value and postoperative recurrence was analyzed by restricted cubic spline graph, y=1-1/(1+e-z) regression equation to build a prediction model. The stability of the model was verified by cross-checking method. RESULTS AND CONCLUSION: (1) Classification of talus osteochondral injury in 154 patients (type I: 36 cases; type II: 37 cases; type III: 40 cases; type IV: 41 cases), T1ρ and T2 values of the four groups were statistically significant (P < 0.05); pairwise comparison was also statistically significant (all P < 0.05). (2) After treatment of 154 patients, 7 cases (4.6%) had local swelling, 3 cases (2.0%) had pain aggravation, and 5 cases (3.3%) had wound infection. There were 2 cases (1.3%) with poor cartilage healing. (3) After treatment, there were statistically significant differences between groups A and B in terms of American Orthopaedic Foot & Ankle Society score, visual analog scale score, plantar flexor motion range, dorsoextension motion range, subchondral bone marrow edema volume, interleukin-6, interleukin-8, C-reactive protein, procalcitonin, platelet-derived growth factor, transforming growth factor-β1, and efficacy (P < 0.05). The total effective rate of group B (90%) was higher than that of group A (85%) (P < 0.05). (4) Age (OR=1.589, 95%CI: 0.305-1.252, P=0.036), interleukin-6 (OR=1.737, 95%CI: 0.974-5.254, P=0.049), interleukin-8 (OR=1.385, 95%CI: 1.066-4.355, P=0.034), C-reactive protein (OR=1.957, 95%CI: 1.323-2.178, P=0.035), transforming growth factor-β1 (OR=1.459, 95%CI: 0.897-2.455, P=0.038), T1-ρ (OR=1.687, 95%CI: 0.854-3.321, P=0.026), T2 (OR=1.843, 95%CI: 0.657- 2.454, P=0.036), complications (OR=1.719, 95%CI: 0.654-3.464, P=0.019), and classification of osteochondral injury of talus (OR=3.789, 95%CI: 1.023-5.897, P=0.028) were independent risk factors for postoperative recurrence. Microfracture surgery (OR=0.751, 95%CI: 0.321-1.264, P=0.012) and autogenous bone and cartilage grafting (OR=0.649, 95%CI: 0.246-1.356, P=0.023) were independent protective factors for recurrence after medium- and long-term follow-up. (5) When T1ρ value ≤35 ms, the risk of postoperative recurrence decreased rapidly, and when T1ρ value > 35 ms, the risk of postoperative recurrence increased rapidly. (6) Further stepwise regression analysis showed that these nine risk factors were most closely associated with postoperative recurrence, and the formula for postoperative recurrence was obtained. The probability of postoperative recurrence was calculated using the regression equation. When P=0.75, the maximum value of Jorden index was 77.728, indicating that the model has a better prediction effect. (7) It is indicated that the quantitative evaluation of T1ρ before operation can effectively guide the selection of surgical methods, improve the success rate of surgery and the quality of life of patients. [ABSTRACT FROM AUTHOR]

Using the think-aloud protocol (TAP), the study investigates the strategies used by beginner and pre-intermediate learners of Chinese when studying Chinese characters through typing. Drawing on concepts from translanguaging, embodiment and LS (learning strategies), a novel notion of translanguaging learning strategies (TLS) and an innovative typology of TLS were proposed. The study found that the pre-intermediate group tended to resort to TLI (translanguaging interdependency) and IE (imagined embodiment) more often than the beginners. TLS for typing were also compared with those for handwriting. While CFL learners mainly relied on HE (haptic embodiment) when typing to learn, a wider range of TLS, including IE, TLI and TSI (trans-semiotising interdependency), were drawn from during handwriting. As a pioneer in situating learning strategies in a translanguaging framework, the study calls for further empirical research to validate the proposal of TLS and its typology. [ABSTRACT FROM AUTHOR]

Compound words exhibit properties of both single words and phrases, raising the question of the extent to which compounds are processed as single units or as word combinations. Most studies have addressed this in Germanic languages (English, German and Dutch) which have the similar compound structure of modifier-head ordering. To see whether this limits our understanding of compound word processing and to examine compound decomposition in another language, we presented Persian stimuli auditorily in a paradigm involving typing out stimuli. We examined the effects of semantic transparency, modifier-head ordering and the potential differences between attached compounds written without spaces and those with a space between the constituents. We report the inter-keystroke-interval times, yielding letterby-letter production of compound structures produced by 31 native speakers of Persian. Results analyzed in a linear mixed-model regression analysis suggested that, for all compounds, typing speed is slowed at the boundary between the constituents of Persian compound words. These effects, which we interpret to be evidence of morphological decomposition, were present for both semantically transparent and opaque compounds, for both head-initial and head-final compounds, and for both attached and spaced compounds. We observed greater morphological decomposition effects in semantically transparent (versus opaque) compounds. We also observed that the way transparency influences the degree of decomposition is moderated by headedness. Thus, this first report for the written production of compound words confirms previous observations of significant decomposition at morphological boundaries in English compounds, but with variation specific to Persian. [ABSTRACT FROM AUTHOR]

This study aimed to identify and characterize isolates of Francisella salimarina associated with an outbreak on a marine fish farm in Brazil and to analyse their genetic variability and antimicrobial susceptibility. In 2021, diseased cobias (Rachycentron canadum, n = 10) and dusky groupers (Epinephelus marginatus, n = 10) were sampled and subjected to bacteriological and pathological examinations. The isolates obtained were morphologically and biochemically characterized and identified by matrix‐assisted laser desorption/ionization‐time of flight (MALDI‐ToF) and 16S rRNA gene sequencing. The genetic diversity of these isolates was analysed using repetitive extragenic palindromic‐polymerase chain reaction (REP‐PCR). Antimicrobial susceptibility was assessed using the disk diffusion technique. Macroscopically, the fish presented skin ulcerations, ocular lesions, hepatomegaly and splenomegaly. A pleomorphic, gram‐negative, catalase‐ and oxidase‐positive bacterium was isolated from seven cobias and two groupers. The 16S rRNA gene sequences showed >99% coverage and identity with other deposited sequences of F. salimarina. The results of the biochemical analysis corresponded to these bacterial species. Histologically, granulomas were observed in the spleen, liver and heart of the cobias (n = 6), and necrotizing and fibrinous dermatitis and myositis were identified in some groupers (n = 2). The isolates exhibited the same banding pattern when REP‐PCR was performed, indicating that they were clonally related. Finally, the antibiogram test, no inhibition halo was observed for amoxicillin and trimethoprim‐sulfamethoxazole. To our knowledge, this is the first report of F. salimarina infection in cobias and dusky groupers. [ABSTRACT FROM AUTHOR]

ABSTRACT Ocular syphilis is a serious complication of Treponema pallidum infection that can occur at any stage of syphilis and affect any eye structure. It remains unknown if certain T. pallidum strains are associated with ocular infections; therefore, we performed genotyping and whole genome sequencing (WGS) to characterize strains from patients with ocular syphilis. Seventy-five ocular or non-ocular specimens from 55 ocular syphilis patients in 14 states within the United States were collected between February 2016 and November 2020. Sufficient T. pallidum DNA was available from nine patients for genotyping and three for WGS. Genotyping was done using the augmented Centers for Disease Control and Prevention typing scheme, and WGS was performed on Illumina platforms. Multilocus sequence typing allelic profiles were predicted from whole genome sequence data. T. pallidum DNA was detected in various specimens from 17 (30.9%) of the 55 patients, and typing was done on samples from 9 patients. Four complete strain types (14d10/g, 14b9/g, 14d9/g, and 14e9/f) and five partial types were identified. WGS was successful on samples from three patients and all three strains belonged to the SS14 clade of T. pallidum. Our data reveal that multiple strain types are associated with ocular manifestations of syphilis. While genotyping and WGS were challenging due to low amounts of T. pallidum DNA in specimens, we successfully performed WGS on cerebrospinal fluid, vitreous fluid, and whole blood.IMPORTANCESyphilis is caused by the spirochete Treponema pallidum. Total syphilis rates have increased significantly over the past two decades in the United States, and the disease remains a public health concern. In addition, ocular syphilis cases has also been on the rise, coinciding with the overall increase in syphilis rates. We conducted a molecular investigation utilizing traditional genotyping and whole genome sequencing over a 5-year period to ascertain if specific T. pallidum strains are associated with ocular syphilis. Genotyping and phylogenetic analysis show that multiple T. pallidum strain types are associated with ocular syphilis in the United States.

Assistive technologies have been developed to enhance blind users’ typing performance, focusing on speed, accuracy, and effort reduction. One such technology is word prediction software, designed to minimize keystrokes required for text input. This study investigates the impact of word prediction on typing performance among blind users using an on-screen QWERTY keyboard. We conducted a comparative study involving eleven blind participants, evaluating both standard QWERTY input and word prediction-assisted typing. Our findings reveal that while word prediction slightly improves typing speed, it does not enhance typing accuracy and increases both physical and temporal workload compared to the default keyboard. We conclude with recommendations for improving word prediction systems, including more efficient editing methods and the integration of voice pitch variations to aid error recognition.

Background. The study of the phylogeography of the tularemia microbe is not only practical, but also of scientific interest, as it allows us to evaluate the evolutionary processes of Francisella tularensis in modern conditions.Aim. Phylogenetic analysis of strains of the causative agent of tularemia in the Rostov region and identification of erythromycin-sensitive genovariants.Results. A representative collection of 122 cultures, isolated from various sources from 1945 to 2022, was studied. As it was established, all the studied strains of the causative agent of tularemia had typical cultural, morphological, and biological characteristics. The results of the study of bacteria antibiograms showed that all strains were resistant to β-lactams, polymyxin, clindamycin, and sensitive to aminoglycosides, rifampicin, and fluoroquinolones. In relation to erythromycin, only 2 erythromycin-sensitive strains were found. Phylogenetic analysis of 34 European and Russian strains of F. tularensis made it possible to determine whether these strains belong to subgroup B.7 of the main genetic group B.6.Conclusion. For the first time, in the Tselinsky district of the Rostov region, the presence of «atypical» genovariants of the tularemia microbe belonging to subgroup B.7 of the main group B.6 of the subspecies holarctica was registered, according to the modern scheme of genetic typing of F. tularensis subsp. holarctica.

C. difficile infection (CDI) has an important impact on both human and animal health. The rapid detection and monitoring of C. difficile PCR-ribotypes (RTs) cause of CDI is critical to control and prevent this infection. This study reports the first application of the Molecular Beacon (MB)-based real-time PCR method in genotyping important C. difficile RTs of the main evolutionary clades. The cdtR gene was used as target and the cdtR sequences were analyzed after extraction from deposited genomes or were obtained after sequencing from strains of different origin. cdtR alleles were identified after sequence comparisons and MB-based real-time PCR assays were developed to discriminate them. In total, 550 cdtR sequences were compared, 38 SNPs were found, and five different cdtR alleles were identified. In total, one or two alleles were associated to the RTs grouped in the same evolutionary clade. A MB-based real-time assay was designed for each allele and for optimized testing of the C. difficile strains. The results obtained demonstrated that the MB-based real-time PCR assays developed in this study represent a powerful, original, and versatile tool to identify C. difficile types/clades and to monitor changes in the population structure of this important pathogen.

Abstract Background Antimicrobial resistance (AMR) of Neisseria gonorrhoeae is a threat to public health as strains have developed resistance to antimicrobials available for the treatment of gonorrhea. Whole genome sequencing (WGS) can detect and predict antimicrobial resistance to enhance the control and prevention of gonorrhea. Data on the molecular epidemiology of N. gonorrhoeae is sparse in Zambia. This study aimed to determine the genetic diversity of N. gonorrhoeae isolated from patients attending sexually transmitted infection (STI) clinics in Lusaka, Zambia. Methods A cross-sectional study that sequenced 38 N. gonorrhoeae isolated from 122 patients with gonorrhea from 2019 to 2020 was conducted. The AMR profiles were determined by the E-test, and the DNA was extracted using the NucliSens easyMaG magnetic device. Whole genome sequencing was performed on the Illumina NextSeq550 platform. The Bacterial analysis pipeline (BAP) that is readily available at: https://cge.cbs.dtu.dk/services/CGEpipeline-1.1 was used for the identification of the species, assembling the genome, multi-locus sequence typing (MLST), detection of plasmids and AMR genes. Phylogeny by single nucleotide polymorphisms (SNPs) was determined with the CCphylo dataset. Results The most frequent STs with 18.4% of isolates each were ST7363, ST1921 and ST1582, followed by ST1583 (13%), novel ST17026 (7.9%), ST1588 (7.9%), ST1596 (5.3%), ST11181 (5.3%), ST11750 (2.6/%) and ST11241 (2.6%) among the 38 genotyped isolates. The blaTeM-1B and tetM (55%) was the most prevalent combination of AMR genes, followed by blaTeM-1B (18.4%), tetM (15.8%), and the combination of blaTeM-1B, ermT, and tetL was 2.6% of the isolates. The AMR phenotypes were predicted in ciprofloxacin, penicillin, tetracycline, azithromycin, and cefixime. The combination of mutations 23.7% was gryA (S91F), parC (E91G), ponA (L421) and rpsJ (V57M), followed by 18.4% in gyrA (S91F), ponA (L421P), rpsJ (V57M), and 18.4% in gyrA (D95G, S91F), ponA (L421P), and rpsJ (V57M). The combinations in gyrA (D95G, S91F) and rpsJ (V57M), and gyrA (D95G, S91F), parC (E91F), ponA (L421P) and rpsJ (V57M) were 13.2% each of the isolates. Plasmid TEM-1 (84.2%), tetM (15.8%), and gonococcal genetic island (GGI) was detected in all isolates. Conclusion This study revealed remarkable heterogeneity of N. gonorrhoeae with bla TEM−1, tetM, ponA, gyrA, and parC genes associated with high resistance to penicillin, tetracycline, and ciprofloxacin demanding revision of the standard treatment guidelines and improved antimicrobial stewardship in Zambia.

In 2009, a large outbreak of leishmaniasis, associated with environmental changes, was declared near Madrid (Spain), in which Phlebotomus perniciosus was the vector, whereas the main reservoirs were hares and rabbits. Analysis of isolates from humans, vectors and leporids from the focus identified the Leishmania infantum ITS-Lombardi genotype. However, multilocus enzyme electrophoresis (MLEE), the reference technique for Leishmania typing, and sequencing of the hsp70 gene, a commonly used marker, were not performed. In the present study, 19 isolates from P. perniciosus (n = 11), hares (n = 5) and rabbits (n = 3) from the outbreak area, all characterized as ITS-Lombardi in previous studies, were analysed by MLEE and hsp70 sequencing. The hsp70 results confirmed that all the analysed strains are L. infantum. However, by MLEE, 4 different zymodemes of L. infantum were identified based on variable mobilities of the NP1 enzyme: MON-34 (NP1100, n = 11), MON-80 (NP1130, n = 6), MON-24 (NP1140, n = 1) and MON-331 (NP1150, n = 1). The relative frequency of these zymodemes does not correspond to their usual occurrence in Spain. Moreover, MON-34 and MON-80 were found in P. perniciosus, hares and rabbits for the first time. These findings continue to provide insights into the outbreak and call for further studies with a higher number of strains.

Staphylococcus aureus is an important pathogen that causes mastitis in cattle, and the emergence of methicillin-resistant S. aureus (MRSA) poses a threat to veterinary and human medicine. The aims of the study were to investigate the prevalence of MRSA and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from clinical mastitis, their ability to form biofilms, and the antimicrobial susceptibility of S. aureus strains. In addition, the Staphylococcal Cassette Chromosome mec (SCCmec) type, spa type and the presence of Panton-Valentine Leucocidin in MRSA were evaluated. A total of 326 staphylococcal strains were screened by multiplex-PCR for S. aureus and Staphylococcus intermedius group (SIG) identification. The S. aureus strains (n = 163) were subjected to phenotypic testing for antimicrobial susceptibility and biofilm formation. Molecular analysis was performed on MRSA mecA-positive strains. Of 163 S. aureus isolates, 142 strains (87.1%) were resistant to at least one antibiotic, and all 19 MRSA strains were resistant to at least four out of five antibiotics tested. All S. aureus strains harboured the icaA gene and were biofilm producers. Nineteen MR-CoNS strains were also isolated. The most prevalent spa types among MRSA were t001 (57.9%) and t037 (31.6%), while one MRSA was type t008 and one was type t041. Most MRSA were SCCmec type I (63.2%) and III (31.6%) and only one strain was type IV. None of the MRSA isolates had the PVL gene. The prevalence of multidrug-resistant S. aureus in bovine mastitis is a serious concern. The finding of MRSA with spa types predominant in humans and infrequent in Italian cows and with SCCmec infrequently found in bovine milk or cheese suggest a human origin of these strains. The ability of MRSA and MR-CoNS involved in bovine mastitis to be transferred to humans and vice versa poses a public health concern. [ABSTRACT FROM AUTHOR]

Cardiac amyloidosis is increasingly recognized as a treatable form of heart failure. Highly effective specific therapies have recently become available for the 2 most frequent forms of cardiac amyloidosis: immunoglobulin light chain amyloidosis and transthyretin (ATTR) amyloidosis. Nevertheless, initiation of specific therapies requires recognition of cardiac amyloidosis and appropriate characterization of the amyloid type. Although noninvasive diagnosis is possible for ATTR cardiac amyloidosis, histological demonstration and typing of amyloid deposits is still required for a substantial number of patients with ATTR and in all patients with light chain amyloidosis and other rarer forms of cardiac amyloidosis. Amyloid histological typing can be performed using different techniques: mass spectrometry, immunohistochemistry, and immunoelectron microscopy. This review describes which patients require histological confirmation of cardiac amyloidosis along with when and how to type amyloid deposits in histologic specimens. Furthermore, it covers the characteristics and limitations of the different typing methods that are available in clinical practice. [Display omitted] • A substantial proportion of patients with CA require tissue sampling for diagnosis, and histological typing can inform the prognosis and help guide treatment. • Histological typing can be achieved by antibody-based or mass spectrometry methods, each of which has advantages and limitations. • Protocols to improve the performance and expand the availability of tissue typing could improve the evaluation and management of patients with suspected amyloid heart disease. [ABSTRACT FROM AUTHOR]

Background: Antimicrobial resistance (AMR) of Neisseria gonorrhoeae is a threat to public health as strains have developed resistance to antimicrobials available for the treatment of gonorrhea. Whole genome sequencing (WGS) can detect and predict antimicrobial resistance to enhance the control and prevention of gonorrhea. Data on the molecular epidemiology of N. gonorrhoeae is sparse in Zambia. This study aimed to determine the genetic diversity of N. gonorrhoeae isolated from patients attending sexually transmitted infection (STI) clinics in Lusaka, Zambia. Methods: A cross-sectional study that sequenced 38 N. gonorrhoeae isolated from 122 patients with gonorrhea from 2019 to 2020 was conducted. The AMR profiles were determined by the E-test, and the DNA was extracted using the NucliSens easyMaG magnetic device. Whole genome sequencing was performed on the Illumina NextSeq550 platform. The Bacterial analysis pipeline (BAP) that is readily available at: https://cge.cbs.dtu.dk/services/CGEpipeline-1.1 was used for the identification of the species, assembling the genome, multi-locus sequence typing (MLST), detection of plasmids and AMR genes. Phylogeny by single nucleotide polymorphisms (SNPs) was determined with the CCphylo dataset. Results: The most frequent STs with 18.4% of isolates each were ST7363, ST1921 and ST1582, followed by ST1583 (13%), novel ST17026 (7.9%), ST1588 (7.9%), ST1596 (5.3%), ST11181 (5.3%), ST11750 (2.6/%) and ST11241 (2.6%) among the 38 genotyped isolates. The blaTeM-1B and tetM (55%) was the most prevalent combination of AMR genes, followed by blaTeM-1B (18.4%), tetM (15.8%), and the combination of blaTeM-1B, ermT, and tetL was 2.6% of the isolates. The AMR phenotypes were predicted in ciprofloxacin, penicillin, tetracycline, azithromycin, and cefixime. The combination of mutations 23.7% was gryA (S91F), parC (E91G), ponA (L421) and rpsJ (V57M), followed by 18.4% in gyrA (S91F), ponA (L421P), rpsJ (V57M), and 18.4% in gyrA (D95G, S91F), ponA (L421P), and rpsJ (V57M). The combinations in gyrA (D95G, S91F) and rpsJ (V57M), and gyrA (D95G, S91F), parC (E91F), ponA (L421P) and rpsJ (V57M) were 13.2% each of the isolates. Plasmid TEM-1 (84.2%), tetM (15.8%), and gonococcal genetic island (GGI) was detected in all isolates. Conclusion: This study revealed remarkable heterogeneity of N. gonorrhoeae with blaTEM−1, tetM, ponA, gyrA, and parC genes associated with high resistance to penicillin, tetracycline, and ciprofloxacin demanding revision of the standard treatment guidelines and improved antimicrobial stewardship in Zambia. [ABSTRACT FROM AUTHOR]

Artificial Intelligence (AI) is an emerging, transforming and revolutionary technology that has captured attention worldwide. It is translating research into precision oncology treatments. AI can analyse large or big data sets requiring high‐speed specialized computing solutions. The data are big in terms of volume and multimodal with the amalgamation of images, text and structure. Machine learning has identified antifungal drug targets, and taxonomic and phylogenetic classification of fungi based on sequence analysis is now available. Real‐time identification tools and user‐friendly mobile applications for identifying fungi have been discovered. Akin to histopathology, AI can be applied to fungal cytology. AI has been fruitful in cytopathology of the thyroid gland, breast, urine and uterine cervical lesions. AI has a huge scope in fungal cytology and would certainly bear fruit with its accuracy, reproducibility and capacity for handling big data. The purpose of this systematic review was to highlight the AI's utility in detecting fungus and its typing with a special focus on future application in fungal cytology. We also touch upon the basics of AI in brief. [ABSTRACT FROM AUTHOR]

C. difficile infection (CDI) has an important impact on both human and animal health. The rapid detection and monitoring of C. difficile PCR-ribotypes (RTs) cause of CDI is critical to control and prevent this infection. This study reports the first application of the Molecular Beacon (MB)-based real-time PCR method in genotyping important C. difficile RTs of the main evolutionary clades. The cdtR gene was used as target and the cdtR sequences were analyzed after extraction from deposited genomes or were obtained after sequencing from strains of different origin. cdtR alleles were identified after sequence comparisons and MB-based real-time PCR assays were developed to discriminate them. In total, 550 cdtR sequences were compared, 38 SNPs were found, and five different cdtR alleles were identified. In total, one or two alleles were associated to the RTs grouped in the same evolutionary clade. A MB-based real-time assay was designed for each allele and for optimized testing of the C. difficile strains. The results obtained demonstrated that the MB-based real-time PCR assays developed in this study represent a powerful, original, and versatile tool to identify C. difficile types/clades and to monitor changes in the population structure of this important pathogen. [ABSTRACT FROM AUTHOR]

Previous molecular studies have shown that Candida africana corresponds to the clade 13 of Candia albicans. It has been mostly involved in vulvovaginal candidiasis worldwide but few data exist in South America. The aim of our study was to investigate the prevalence of C. africana in women living in French Guiana. For this, we first set up a fluorescent-intercalating-dye-real time Polymerase Chain Reaction (PCR) targeting the hyphal wall protein 1 gene. The test was applied to 212 C. albicans isolates collected from May to August 2019 from vaginal swabs, allowing the identification of six women harboring C. africana (eight isolates). The in vitro susceptibility of these eight isolates to six antifungal drugs was also evaluated. No demographics or clinical-specific features could be demonstrated. Genetic diversity of those isolates was analyzed through multilocus sequence typing and showed that diploid sequence type 182 was predominant (n  = 6) and allowed the report of a new diploid sequence type. [ABSTRACT FROM AUTHOR]

Purpose: A new high-resolution next-generation sequencing (NGS)-based method was established to type closely related European type II Toxoplasma gondii strains. Methods: T. gondii field isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). In comparison to ME49 (a type II reference strain), highly polymorphic regions (HPRs) were identified, showing a considerable number of single nucleotide polymorphisms (SNPs). After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel for multiplex PCR to establish a multilocus Ion AmpliSeq typing method. Toxoplasma gondii isolates and T. gondii present in clinical samples were typed with the new method. The sensitivity of the method was tested with serially diluted reference DNA samples. Results: Among type II specimens, the method could differentiate the same number of haplotypes as the reference standard, microsatellite (MS) typing. Passages of the same isolates and specimens originating from abortion outbreaks were identified as identical. In addition, seven different genotypes, two atypical and two recombinant specimens were clearly distinguished from each other by the method. Furthermore, almost all SNPs detected by the Ion AmpliSeq method corresponded to those expected based on WGS. By testing serially diluted DNA samples, the method exhibited a similar analytical sensitivity as MS typing. Conclusion: The new method can distinguish different T. gondii genotypes and detect intra-genotype variability among European type II T. gondii strains. Furthermore, with WGS data additional target regions can be added to the method to potentially increase typing resolution. [ABSTRACT FROM AUTHOR]

In a society where people often communicate through digital technology, it is crucial to investigate whether the transmission mode influences destination memory performance (our capacity to remember to whom we transmitted certain information). In Experiment 1, we asked young adults (N = 31) to share of a set of familiar proverbs only by typing and the rest by both typing and saying them aloud. Better destination memory was observed when the information was transmitted by the two means (aloud and typing). Did this better performance occur because participants shared the information aloud or because the information was transmitted by two means? In Experiment 2, young adults (N = 34) shared familiar proverbs aloud, by typing, or simultaneously aloud and by typing. Results showed that transmission aloud led to a better destination memory than typing, and no further improvement occurred when the transmission was both aloud and by typing. Additionally, no differences were observed regarding item memory, supporting the idea that item and destination memories are two different types of memory. [ABSTRACT FROM AUTHOR]

Knowledge about writers' eye movements and their effects on the writing process, and its product—the finally edited text—is still limited. Previous research has demonstrated that there are differences between reading texts written by someone else and reading one's own emerging text and that writers frequently look back into their own texts (Torrance et al. in Psychol Res Psychologische Forschung 80(5):729–743, 2016. https://doi.org/10.1007/s00426-015-0683-8). For handwriting, Alamargot et al. (Writing and cognition: research and applications. Elsevier Science, pp 13–29, 2007) found support that these lookbacks could occur in parallel with transcription, but to our knowledge this type of parallel processing has not been explored further, and definitely not in the context of computer writing. Considering that language production models are moving away from previous sequential or serial models (e.g., Levelt in Speaking from intentions to articulation. MIT Press, 1989) towards models in which linguistic processes can operate in parallel (Olive in J Writ Res, 2014. https://doi.org/10.17239/jowr-2014.06.02.4), this is slightly surprising. In the present paper, we introduce a methodological approach to examine writers' parallel processing in which we take our point of departure in visual attention rather than in the keystrokes. Capitalizing on New ScriptLog's feature to link gaze with typing across different functional units in the writing task, we introduce and describe a method to capture and examine sequences of typing during fixations, outline how these can be examined in relation to each other, and test our approach by exploring typing during fixations in a text composition task with 14 competent adult writers. [ABSTRACT FROM AUTHOR]

Wild animals in general, birds in particular, play a key role in transporting ticks and propagating tick-borne pathogens. Several studies have confirmed the infection of birds with Anaplasma phagocytophilum, with overall prevalence varying widely from country to country and/or study to study. This zoonotic bacterium, transmitted mainly by ticks of the genus Ixodes, is responsible for granulocytic anaplasmosis in humans (HGA) and domestic animals (cats, dogs, horses). The disease is also called tick-borne fever (TBF) in ruminants. Extremely rare in the USA, TBF is very common in Europe, where it causes economic losses in livestock. Conversely, HGA is well established in the USA whereas only a few less severe cases have been observed in Europe. Current typing techniques support the existence of multiple variants with differences in virulence/pathogenicity and tropism for certain tick and host species. However, epidemiological cycles remain difficult to characterize in Europe. Several studies describe a cycle apparently involving only birds in Europe, but no such study has been conducted in mainland France. Our objectives were to search for A. phagocytophilum in passerine birds in the Ile-de-France region and to explore their diversity using groEL and ankA gene typing and multilocus sequence typing (MLST). Various tissues (spleen, liver, and skin) were collected from cadavers of 680 passerines between March and December 2021. The presence of A. phagocytophilum was detected by qPCR Taqman targeting the msp2 gene. Three blackbirds (Turdus merula) were found positive, representing detection rates of 0.4 % in all birds tested and 3.3 % in blackbirds. The higher frequency of detection in blackbirds could be at least partially explained by their lifestyle, as they feed on the ground. Analysis of the results of groEL and ankA typing and MLST from positive blackbirds support the hypothesis that the avian A. phagocytophilum strains in Ile-de-France are distinct from those found in mammals, and that they form their own cluster in Europe.

Compound words exhibit properties of both single words and phrases, raising the question of the extent to which compounds are processed as single units or as word combinations. Most studies have addressed this in Germanic languages (English, German and Dutch) which have the similar compound structure of modifier-head ordering. To see whether this limits our understanding of compound word processing and to examine compound decomposition in another language, we presented Persian stimuli auditorily in a paradigm involving typing out stimuli. We examined the effects of semantic transparency, modifier-head ordering and the potential differences between attached compounds written without spaces and those with a space between the constituents. We report the inter-keystroke-interval times, yielding letter-by-letter production of compound structures produced by 31 native speakers of Persian. Results analyzed in a linear mixed-model regression analysis suggested that, for all compounds, typing speed is slowed at the boundary between the constituents of Persian compound words. These effects, which we interpret to be evidence of morphological decomposition, were present for both semantically transparent and opaque compounds, for both head-initial and head-final compounds, and for both attached and spaced compounds. We observed greater morphological decomposition effects in semantically transparent (versus opaque) compounds. We also observed that the way transparency influences the degree of decomposition is moderated by headedness. Thus, this first report for the written production of compound words confirms previous observations of significant decomposition at morphological boundaries in English compounds, but with variation specific to Persian.

ObjectiveTo provide a scientific basis for the prevention and control of foodborne diseases in Chenzhou， the etiological characteristics and epidemiological patterns of foodborne diseases were analyzed.MethodsThe Case information and stool and anal swab samples were collected from two sentinel hospitals in Chenzhou in 2017 and 2019. According to the methods described in “National Manual of Foodborne Disease Surveillance”， the samples were tested for pathogens， pathogen typing， and drug sensitivity.ResultsA total of 825 samples of diarrhea cases were collected， and the total detection rate of pathogens was 30.18% （249/825）， including 16.24% （134/825） Salmonella， 11.76% （97/825） Norovirus， 3.52% （29/825） diarrheal Escherichia coli， 0.73% （6/825） Vibrio parahaemolyticus， and 0.12% （1/825） Shigella. Bacterial detection rates were higher in the second and third quarters than in other quarters， and viral detection rates were higher in the first and fourth quarters than in other quarters. The highest pathogen detection rate was 40.79% （31/76） in the 2-6-year-old group. Milk and dairy products， grains and their products， and fruits and their products were suspected foods. The highest detection rate in Salmonella was in Salmonella enterica subsp.（74.63%， 100/134）， the highest detection rate in diarrheal Escherichia coli was in intestinal adhesion type and enterotoxin type （34.48%， 10/29）， and the norovirus was mainly GII type （85.57%， 83/97）. The highest resistance rate of Salmonella to tetracycline was 88.71% （110/124）， and the multidrug resistance rate of Salmonella was 85.48% （106/124）. The resistance rate of diarrheal Escherichia coli to ampicillin was significant （79.31%， 23/29）， and the multidrug resistance rate of diarrheal Escherichia coli was 62.07% （18/29）.ConclusionThe main pathogens of diarrheal cases of foodborne diseases were Salmonella and Norovirus in Chenzhou. Salmonella and diarrheal Escherichia coli are highly resistant to antibiotics. Therefore， it is necessary to conduct targeted food safety supervision， strengthen antibiotic resistance monitoring， and strictly prevent antibiotic abuse.

This article describes the development of software to identify a user based on his or her keystrokes. Biometric authentication, particularly methods based on behavioral characteristics, is becoming increasingly popular. This is due to its ability to provide security without the need to remember passwords. The development of a biometric identification system based on the typing of a text entered by the user on the keyboard is an attractive solution, as it not only has the potential to provide a fairly reliable identification, but also does not require significant costs for special equipment. This paper discusses parameters that affect typing, including keystroke time, typing speed, error rates, and others that depend on individual user characteristics. The paper also discusses the drawbacks of biometric identification, particularly the impact of external factors such as fatigue or distraction on authentication accuracy. Based on the analysis of parameters, known methods and approaches to biometric identification by keystroke style, a new verification algorithm is proposed. It is based on the analysis of the time intervals between keystrokes and key searches, which allows to determine the correspondence between the stored user profile and the current keystroke. In order to reduce the influence of external factors, the use of a fixed text of at least 300 characters is recommended. The effectiveness of the developed software based on the proposed identification algorithm is confirmed by the results of the experiments conducted with the developed software application. The results show sufficient reliability and accuracy in the identification process. The work is of practical importance for the development of new security methods in information technology and opens new opportunities for the implementation of biometric systems in various fields. [ABSTRACT FROM AUTHOR]

Students must be able to produce legible and fluent text when completing classwork and for exam purposes. Some students, however, present with handwriting difficulties in secondary school. When these are significant, intervention may be necessary or alternatives to handwriting may be offered (e.g. use of a word processor). Little is known about current practice of supporting secondary students with handwriting difficulties in England and how recommendations are made to transition to typing. Semi‐structured interviews were conducted with 13 practitioners with a responsibility for supporting students with handwriting difficulties. Two themes were identified. The first theme, 'doing the right thing', illustrated the tension between practitioners' commitment to supporting students with handwriting difficulties and their uncertainty around what is the 'right' approach. The second theme, 'influencing practice', described the contextual factors (student and family, school environment and national context) that impact on practitioners' practice and their decision to transition from handwriting to typing. Findings highlight the complexities of supporting this group of students and an urgent need for guidance at a national level to assist best practice. Implications for practice are discussed. Further research examining the effectiveness of handwriting interventions with secondary students and the optimum time to start typing is warranted. [ABSTRACT FROM AUTHOR]

BACKGROUND: Sedentary work may lead to low back pain. In particular, a slumped sitting position may exacerbate low back pain because of tissue damage caused by excessive lumbar flexion and posterior pelvic tilting. Subjects with low back pain may have excessive changes in the lumbopelvic posture and back muscle activity in the sitting position. OBJECTIVE: The purpose of this study was to compare the effects of vibration-based biofeedback using a motion sensor belt and no biofeedback on multifidus (MF) muscle activity and pelvic tilt angle during typing. METHODS: Thirty subjects with low back pain accompanied by hip flexion limitation (15 each in the biofeedback and non-biofeedback groups) were enrolled. Electromyography was used to investigate MF muscle activity before and after typing for 30 min. Pelvic tilt was measured after typing in a sitting position for 30 min. Independent t-tests were used to compare MF muscle activity, and pelvic and second sacrum tilt angles, between the biofeedback and non-biofeedback groups. RESULTS: After typing for 30 min, changes in MF muscle activity (11.45% and - 7.19% for the biofeedback and nonbiofeedback groups, respectively) and pelvic and second sacrum tilt angles (3.15 ∘ and 4.12 ∘ for the biofeedback group and - 11.05 ∘ and - 18.16 ∘ for the non-biofeedback group, respectively) were significantly smaller in the biofeedback than non-biofeedback group (p < 0.05). CONCLUSION: Vibration-based biofeedback minimizes the reduction in MF muscle activity and changes in pelvic and second sacrum tilt angles during typing in individuals with low back pain accompanied by hip flexion limitation. [ABSTRACT FROM AUTHOR]

Portal vein thrombosis is one of the common complications of liver cirrhosis. The incidence of portal vein thrombosis is increased with the progression of diseases. The incidence and progression of portal vein thrombosis are associated with multiple factors. The indications of anticoagulant therapy remain to be investigated. At present, portal vein thrombosis is no longer considered as a contraindication for liver transplantation. Nevertheless, complicated portal vein thrombosis will increase perioperative risk of liver transplantation. How to restore the blood flow of portal vein system is a challenge for surgical decision-making in clinical practice. Rational preoperative typing, surgical planning and portal vein reconstruction are the keys to ensure favorable long-term prognosis of liver transplant recipients. In this article, epidemiological status, risk factors, typing and identification of portal vein thrombosis, preoperative and intraoperative management of portal vein thrombosis in liver transplantation, and the impact of portal vein thrombosis on the outcomes of liver transplantation were reviewed, aiming to provide reference for perioperative management of portal vein thrombosis throughout liver transplantation. [ABSTRACT FROM AUTHOR]

One of the most common interests among cognitive psychologists is establishing ways to enhance human learning. An additional layer of complexity has been brought on by the rapid evolution of technology. Specifically, examining if the mechanisms involved in typing differ from those involved in handwriting. The literature concerning the implications of encoding modality on memory have been inconclusive. This present research examined whether encoding modality resulted in performance differences for word recall. Wammes et al.'s (2016) drawing versus handwriting methodology was utilized with the addition of a typing condition. The results replicated the drawing effect, whereby drawn words were better recalled than handwritten ones. Overall, the evidence did not suggest that the mechanisms involved in handwriting led to better free recall than those involved in typing. However, if the pen is indeed mightier than the keyboard (Mueller & Oppenheimer, 2014), then the effect is not explained by visual attention or sensorimotor action differences between modalities. Implications for education are discussed. [ABSTRACT FROM AUTHOR]

Typing slows at the middle of the word. The exact nature of the slowdown is still disputed. Research on attentional and motoric effects in typing suggests that the slowdown is purely a function of chunking of letters in creating the motor output; this approach posits no further influence of linguistic information during output. Research from a psycholinguistic perspective does posit lexical and sublexical effects during output and explains the midword slowing as a function of slowdowns at the boundaries of sublexical units. Across four experiments, using three different typing tasks, we investigated the typing of compound (schoolteacher) and pseudocompound (carpet) words. Typing at the midword region is sensitive to themorphological structure of the word and to linguistic properties of the word and its (pseudo)constituents (e.g., linguistic information about school and teacher affects schoolteacher, and car and pet affects carpet). These findings suggest that typing compounds involves a hierarchical plan consisting of two separatemotor plans for each constituent executed sequentially such that the output of letters is sensitive to the number of letters within that plan, the position of the sequence in the hierarchy (e.g., first vs. second constituent), and the morphemic structure of the tobe-typed word. Surprisingly, given that pseudocompound lexical representations should not include the pseudoconstituents and given that our tasks in the first three experiments demand full access to the lexical representation before typing, pseudocompound typing is also sensitive to the pseudoconstituent characteristics, suggesting that, during typing, the system attempts to build a compound-like structure. [ABSTRACT FROM AUTHOR]

One hundred Actinobacillus pleuropneumoniae (App) and sixty Pasteurella multocida subsp. multocida serogroup A (PmA) isolates were recovered from porcine pneumonic lungs collected from eight central or southern states of Brazil between 2014 and 2018 (App) or between 2017 and 2021 (PmA). A. pleuropneumoniae clinical isolates were typed by multiplex PCR and the most prevalent serovars were 8, 7 and 5 (43, 25% and 18%, respectively). In addition, three virulence genes were assessed in P. multocida isolates, all being positive to capA (PmA) and kmt1 genes, all negative to capD and toxA, and most of them (85%) negative to pfhA gene. The susceptibility of both pathogens to tildipirosin was investigated using a broth microdilution assay. The percentage of isolates susceptible to tildipirosin was 95% for App and 73.3% for PmA. The MIC50 values were 0.25 and 1 μg/mL and the MIC90 values were 4 and >64 μg/mL for App and PmA, respectively. Finally, a multiple-dose protocol of tildipirosin was tested in suckling piglets on a farm endemic for both pathogens. Tildipirosin was able to prevent the natural colonization of the tonsils by App and PmA and significantly (p < 0.0001) reduced the burden of Glaesserella parasuis in this tissue. In summary, our results demonstrate that: (i) tildipirosin can be included in the list of antibiotics to control outbreaks of lung disease caused by App regardless of the capsular type, and (ii) in the case of clinical strains of App and PmA that are sensitive to tildipirosin based on susceptibility testing, the use of this antibiotic in eradication programs for A. pleuropneumoniae and P. multocida can be strongly recommended. [ABSTRACT FROM AUTHOR]

Background: Keyboarding (Typing) is a major writing mode in educational settings in addition to, or as an alternative to, handwriting. Therefore, it is important that occupational therapists become experts on this activity, to support students' performance. Yet, the knowledge of keyboarding performance of elementary-school students, and the underlying functions it entails, is limited. Aim: To compare keyboarding performance (speed and accuracy) of 4th-grade students in copying and dictation keyboarding tasks, and to examine the role of underlying functions in predicting keyboarding performance. Material and Methods: The sample consisted of 57 4th-grade students, recruited from 2 elementary schools. Students were tested for reading speed, attention shifting, fine-motor skills, kinaesthetic awareness, and keyboarding performance. Results: Keyboarding performance differed in the copying and dictation tasks. Reading speed was the major underlying function predicting keyboarding performance in both tasks. Additionally, kinaesthetic awareness had a low, negative correlation with dictation accuracy. Conclusions: When occupational therapists assess students' keyboarding performance, they should use various tasks. Additionally, therapists should consider students' reading speed and kinaesthetic awareness, as they may explain keyboarding performance. This knowledge may also support decision-making when considering keyboarding as an alternative writing mode for students with handwriting difficulties. [ABSTRACT FROM AUTHOR]

Knowing the motor branches and variations of the musculocutaneous nerve to the muscles along its course will facilitate the treatment of flexor spasticity and supracondylar fractures of the humerus in order to minimize nerve lesion. In fetal cadavers, the purpose of our study was to determine the number and course of the formation variations and motor branches of the musculocutaneous nerve. The significance of studying fetal nerve variations is due to injury to the brachial plexus roots during birth. Our study was conducted using the anatomical dissection technique on 102 upper limbs from 51 fetuses ages ranged from 17 to 40 weeks. Throughout its course, the variations and motor branches of the musculocutaneous nerve were analyzed. In 13.7% of cases, the musculocutaneous nerve did not pierce the coracobrachialis. The musculocutaneous nerve gave the muscles 1–3 motor branches. Additionally, motor branches terminated with 1–7 fringes. The biceps brachii motor branches of the musculocutaneous nerve were typed. Accordingly, 15.6% were type 1A, 3.9% were type 1B, 35.4% were type 1C, and 19.6% were type 1D. It was determined that 23.5% of the extremities were type 2 and that 1.9% were type 3. The distance between the musculocutaneous nerve's motor branches and the acromion was proportional to the arm's length. There were no statistically significant differences between the sides and genders for any measurement. Our study's findings will aid in the diagnosis and treatment of pediatrics, orthopedics, surgical sciences, and radiology conditions. It reduces the risk of iatrogenic injury and postoperative complications. We also believe that our research will serve as a resource for anatomists and other scientists. [ABSTRACT FROM AUTHOR]

Objective To assess the relationship between pathological features of chronic gastritis and type Ⅰ Helicobacter pylori（Ⅰ-Hp) and type Ⅱ Helicobacter pylori（Ⅱ-Hp) and the value in clinical application. Methods 2 583 samples were selected from patients diagnosed with chronic gastritis by gastric mucosal biopsy. Hp was detected and typed by fluorescence quantitative PCR. The relationship between Ⅰ-Hp and Ⅱ-Hp， and the degree of inflammatory response， activity， intestinal metaplasia and lymphatic follicular formation in chronic gastritis was analyzed. Results There were significant differences in inflammatory response， activity and intestinal metaplasia in chronic gastritis between Ⅰ-Hp and Ⅱ-Hp infection (all P < 0.05) . Inflammatory activity was an independent risk factor for Ⅰ-Hp infection (P < 0.05) . Conclusions Ⅰ-Hp and Ⅱ-Hp infection have different manifestations in inflammatory response， activity and intestinal metaplasia in chronic gastritis. Active inflammation suggests the potential incidence of Ⅰ-Hp infection. [ABSTRACT FROM AUTHOR]

Background Extended sedentary behavior is a risk factor for chronic disease and mortality, even among those who exercise regularly. Given the time constraints of incorporating physical activity into daily schedules, and the high likelihood of sitting during office work, this environment may serve as a potentially feasible setting for interventions to reduce sedentary behavior. Methods and Results A randomized cross‐over clinical trial was conducted at an employee wellness center. Four office settings were evaluated on 4 consecutive days: stationary or sitting station on day 1 (referent), and 3 subsequent active workstations (standing, walking, or stepper) in randomized order. Neurocognitive function (Selective Attention, Grammatical Reasoning, Odd One Out, Object Reasoning, Visuospatial Intelligence, Limited‐Hold Memory, Paired Associates Learning, and Digit Span) and fine motor skills (typing speed and accuracy) were tested using validated tools. Average scores were compared among stations using linear regression with generalized estimating equations to adjust standard errors. Bonferroni method adjusted for multiple comparisons. Healthy subjects were enrolled (n=44), 28 (64%) women, mean±SD age 35±11 years, weight 75.5±17.1 kg, height 168.5±10.0 cm, and body mass index 26.5±5.2 kg/m2. When comparing active stations to sitting, neurocognitive test either improved or remained unchanged, while typing speed decreased without affecting typing errors. Overall results improved after day 1, suggesting habituation. We observed no major differences across active stations, except decrease in average typing speed 42.5 versus 39.7 words per minute with standing versus stepping (P=0.003). Conclusions Active workstations improved cognitive performance, suggesting that these workstations can help decrease sedentary time without work performance impairment. Registration URL: https://www.clinicaltrials.gov; Unique identifier: NCT06240286.

Streptococcus suis is a bacterial gram-positive pathogen that causes invasive infections in swine and is also a zoonotic disease agent. Traditional molecular typing techniques such as ribotyping, multilocus sequence typing, pulse-field gel electrophoresis, or randomly amplified polymorphic DNA have been used to investigate S. suis population structure, evolution, and genetic relationships and support epidemiological and virulence investigations. However, these traditional typing techniques do not fully reveal the genetically heterogeneous nature of S. suis strains. The high-resolution provided by whole-genome sequencing (WGS), which is now more affordable and more commonly available in research and clinical settings, has unlocked the exploration of S. suis genetics at full resolution, permitting the determination of population structure, genetic diversity, identification of virulent clades, genetic markers, and other bacterial features of interest. This approach will likely become the new gold standard for S. suis strain typing as WGS instruments become more widely available and traditional typing techniques are gradually replaced.

Vibrio parahaemolyticus is a significant cause of foodborne illness, and its incidence worldwide is on the rise. It is thus imperative to develop a straightforward and efficient method for typing strains of this pathogen. In this study, we conducted a pangenome analysis of 75 complete genomes of V. parahaemolyticus and identified the core gene mtlA with the highest degree of variation, which distinguished 44 strains and outperformed traditional seven-gene-based MLST when combined with aer, another core gene with high degree of variation. The mtlA gene had higher resolution to type strains with a close relationship compared to the traditional MLST genes in the phylogenetic tree built by core genomes. Strong positive selection was also detected in the gene mtlA (ω > 1), representing adaptive and evolution in response to the environment. Therefore, the panel of gene mtlA and aer may serve as a tool for the typing of V. parahaemolyticus, potentially contributing to the prevention and control of this foodborne disease.