Search

Your search keyword '"UMT Protection des abeilles dans l’environnement (UMT PrADE)"' showing total 14 results
Start Over Author "UMT Protection des abeilles dans l’environnement (UMT PrADE)"
14 results on '"UMT Protection des abeilles dans l’environnement (UMT PrADE)"'

1. How semi-natural habitats affect the lifespan and foraging behavior of individual honey bees in farmlands?

Author

Verrier, Elise, Bretagnolle, Vincent, Aupinel, Pierrick, Decourtye, Axel, Henry, Mickael, Rebaudo, F., Requier, Fabrice, Laboratoire Evolution, Génome, Comportement et Ecologie, Université Paris-Saclay, CNRS 9191, IRD 247, Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), LTSER «Zone Atelier Plaine & Val de Sevre» [France], Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Abeilles, Paysages, Interactions et Systèmes de culture (APIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de l'abeille (ITSAP), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Abeilles et Environnement (AE)

Subjects
[SDE]Environmental Sciences
Abstract

International audience; Agricultural intensification, promoted by the Common Agricultural Policy over the past decades, led to important declines of biodiversity in European farmlands. In particular, the simplification of agricultural landscapes is known to affect insect pollinators, although paradoxically the yield of numerous crops depends on their survival. The Western honey bee (Apis mellifera) is one of the most important pollinators in farmlands. However, honey bee populations suffer high mortality rates, partly related to the loss of semi-natural habitats leading to a decrease in flower diversity and to periods of food shortage. While some studies assessed the link between semi-natural habitats and honey bee mortality at the colony level, it remains poorly investigated at the individual level. Individual worker bees play a critical role in the colony given that they can adjust their life history and foraging behavior to ensure sufficient intake of food according to colony needs. Therefore, assessing whether the loss of semi-natural habitats disturbs the lifespan and foraging behavior of individual honey bees could help to understand the mechanisms underlying colony mortality. To do so, we selected 14 sites along a landscape gradient of semi-natural habitats in a farmland system, in Western France. We monitored the life history of 1427 newly emerged worker honey bees in the study sites over periods of mass-flowering (April and July) or food shortage (May and June). We used the automatic RFID device (Radio Frequency IDentification) to track their lifespan, their flight activity and their allocation to foraging in the different landscapes. We found noticeable effects of semi-natural habitats on the lifespan and foraging behavior of individual honey bees that would help to understand how individual workers adapt their own life history traits to sustain food intake in landscapes with reduced flower availability. These results will be discussed with the general objective to provide recommendations of landscape management to safeguard insect pollinators in farmlands.

Published
2022

2. Seasonal and environmental variations influencing the Varroa Sensitive Hygiene trait in the honey bee

Author

Léa Tison, Alban Maisonnasse, Fanny Mondet, Yves Le Conte, Clémence Riva, Maxime R. Hervé, André Kretzschmar, Santé et agroécologie du vignoble (UMR SAVE), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Association pour le Developpement de l'Apiculture Provencale (ADAPI), Biostatistique et Processus Spatiaux (BioSP), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
Veterinary medicine, [SDV]Life Sciences [q-bio], hygienic behavior, Honey bee, Biology, behavioral immunity, biology.organism_classification, host-parasite interaction, Insect Science, Varroa destructor, [SDE]Environmental Sciences, Trait, Varroa sensitive hygiene, Apis mellifera
Abstract

International audience; The invasive miteVarroa destructor is identified as the main biotic cause of European honey bee colony losses in many regions, leading to systematic treatments of colonies every year in order to prevent colonies from collapsing. However, some colonies have been reported to survive in the absence of treatment. The ability of honey bee colonies to survive varroa mite infestations has been associated with the development of Varroa Sensitive Hygiene (VSH) behavior. Colonies displaying VSH are able to detect the presence of varroa through the cap of developing brood cells and to remove the parasitized brood. To improve breeding programs for varroa resistance in apiculture, detailed knowledge of the mechanisms that enable bees to survive mite infestation and environmental conditions that can influence the expression and phenotyping of VSH behavior are needed. This study evaluated the influence of the genetic origin of the colony, the colony population dynamics, varroa density, and food supply on the ability of colonies to express the VSH trait. First, we found that varroa-selected colonies displayed lower varroa population growth rate. We also revealed that the genetic origin of the colonies and the month during which the test was performed had a significant impact on VSH behavior, with varroa-selected colonies showing higher VSH abilities than unselected colonies, and especially at the end of summer. Finally, we showed that sedentary varroa-selected colonies expressed higher VSH activity than colonies placed on lavender fields during active honey flow. Such findings are particularly important to standardize testing for varroa resistant colonies in different locations, a feature that is essential to ensure the success of breeding efforts.

Published
2022
Full Text
View/download PDF

3. Pollinator conservation in the context of global changes with a focus on France and Belgium

Author

Savajol Colette, Geslin Benoît, Michez Denis, Henry Mickael, Bertrand Schatz, Allier Fabrice, Drossart Maxime, Gérard Maxence, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of Mons [Belgium] (UMONS), Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut de l'abeille (ITSAP), Department of Zoology, Stockholm University, Stockholm University, Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des Abeilles dans l'Environnement, UMT PrADE, and Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille)

Subjects
0106 biological sciences, media_common.quotation_subject, [SDE.MCG]Environmental Sciences/Global Changes, Face (sociological concept), Political action, Global and local assessment, Context (language use), 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, Keystone species, Pollinator, Pollinator decline, Political science, Perception, Regional science, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Nature and Landscape Conservation, media_common, 0303 health sciences, Ecology, 15. Life on land, Focus (linguistics), European countries, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Biodiversity governance, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; The decline of pollinators has been demonstrated scientifically and this phenomenon is widely recognized by both the general public and by stakeholders. Since pollinators face different threats that are all linked to human activities, there is a unique and unprecedented responsibility for people to conserve pollinators, requiring political action to counter the substantial worldwide risk of pollinator loss. As our perception of the situation is rapidly changing, as a result of the steady accumulation of international and national reports as well as new scientific findings, we propose here to provide an updated overview of pollinator conservation globally. We present the key messages and the proposed solutions found in international reports and assessments, how European countries have interpreted these solutions proposed in the context of existing international frameworks. Next, we analyze how scientific research is addressing the issue of pollinator conservation through different international, European and national programs. The analysis of the keywords used in published scientific articles also allows us to characterize how the scientific community has engaged with this issue over time. Finally, we focus on how France and Belgium have reacted to the observed decline of pollinators, and examine their national interpretations, conservation actions and research contributions.

Published
2021
Full Text
View/download PDF

4. Complex population structure and haplotype patterns in Western Europe honey bee from sequencing a large panel of haploid drones

Author

Yves Le Conte, Emmanuelle Labarthe, Jean Pierre Bidanel, Benjamin Basso, M. Alice Pinto, Melanie Parejo, Sonia E. Eynard, Cecilia Costa, Kamila Canale-Tabet, Olivier Bouchez, Aleš Gregorc, Per Kryger, David Wragg, Małgorzata Bieńkowska, Alain Vignal, Bertrand Servin, Kaspar Bienefeld, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The Roslin Institute, UMT PrADE, ITSAP-Institut de l'Abeille, ITSAP, Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Bee Research Institute, Partenaires INRAE, Research Institute of Horticulture, Division of apiculture, Research Centre for Agriculture and Environment (CREA), University of Maribor, Aarhus University [Aarhus], Swiss Bee Research Centre, University of the Basque Country [Bizkaia] (UPV/EHU), Instituto Politécnico de Bragança, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Vignal, Alain, Biotechnology and Biological Sciences Research Council (BBSRC), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics (CREA), and University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU)

Subjects
education.field_of_study, Population, Haplotype, Population genetics, [SDV.GEN.GA] Life Sciences [q-bio]/Genetics/Animal genetics, Honey bee, Subspecies, Biology, Genome, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, Evolutionary biology, Ploidy, education, Genetic association
Abstract

Honey bee subspecies originate from specific geographic areas in Africa, Europe and the Middle East. The interest of beekeepers in specific phenotypes has led them to import subspecies to regions outside of their original range. The resulting admixture complicates population genetics analyses and population stratification can be a major problem for association studies. As a typical example, the case of the French population is studied here. We sequenced 870 haploid drones for SNP detection and identified nine genetic backgrounds in 629 samples. Five correspond to subspecies, two to isolated populations and two to human-mediated population management. We also highlight several large haplotype blocks, some of which coincide with the position of centromeres. The largest is 3.6 Mb long on chromosome 11, representing 1.6 % of the genome and has two major haplotypes, corresponding to the two dominant genetic backgrounds identified.

Published
2021
Full Text
View/download PDF

5. Pesticide risk assessment in honeybees: Toward the use of behavioral and reproductive performances as assessment endpoints

Author

Julie Fourrier, Lena Barascou, Mickaël Henry, Yves Le Conte, Cédric Alaux, Axel Decourtye, Jean-Luc Brunet, Luc P. Belzunces, Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT PrADE, ITSAP-Institut de l'Abeille, ITSAP, European Project, UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de l'abeille (ITSAP), and European Project: 773921,Horizon 2020,PoshBee(2018)

Subjects
Environmental Engineering, Health, Toxicology and Mutagenesis, 0208 environmental biotechnology, Population, Exploratory research, Context (language use), Regulatory test methods, 02 engineering and technology, 010501 environmental sciences, Biology, Toxicology, Ecotoxicology, 01 natural sciences, Pesticide toxicity, Risk Assessment, Environmental health, Sublethal endpoints, Environmental Chemistry, Animals, Humans, Relevance (information retrieval), Pesticides, education, 0105 earth and related environmental sciences, education.field_of_study, Reproduction, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, Bees, Pollution, people.cause_of_death, Pesticide risk assessment, 020801 environmental engineering, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Apis mellifera, people, Hazard assessment, Reproductive effects
Abstract

International audience; The growing gap between new evidence of pesticide toxicity in honeybees and conventional toxicological assays recommended by regulatory test guidelines emphasizes the need to complement current lethal endpoints with sublethal endpoints. In this context, behavioral and reproductive performances have received growing interest since the 2000s, likely due to their ecological relevance and/or the emergence of new technologies.We review the biological interests and methodological measurements of these predominantly studied endpoints and discuss their possible use in the pesticide risk assessment procedure based on their standardization level, simplicity and ecological relevance. It appears that homing flights and reproduction have great potential for pesticide risk assessment, mainly due to their ecological relevance. If exploratory research studies in ecotoxicology have paved the way toward a better understanding of pesticide toxicity in honeybees, the next objective will then be to translate the most relevant behavioral and reproductive endpoints into regulatory test methods. This will require more comparative studies and improving their ecological relevance. This latter goal may be facilitated by the use of population dynamics models for scaling up the consequences of adverse behavioral and reproductive effects from individuals to colonies.

Published
2021
Full Text
View/download PDF

6. Chemical detection triggers honey bee defense against a destructive parasitic threat

Author

Dominique Beslay, Guy Costagliola, Fanny Mondet, Alison R. Mercer, Yves Le Conte, Célia Bordier, Nicolas Barthes, Benoit Lapeyre, Benjamin Basso, Maxime R. Hervé, Solene Blanchard, Seo Hyun Kim, Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Otago [Dunedin, Nouvelle-Zélande], UMT PrADE, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille), Fondation de France (France - New Zealand Friendship Fund) French Ministry of Agriculture (CASDAR, Mosar) NZIDRS, UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Institut de l'abeille (ITSAP)

Subjects
Beekeeping, Varroidae, Defence mechanisms, Zoology, Biology, Acetates, Complex Mixtures, Host-Parasite Interactions, 03 medical and health sciences, Animals, Molecular Biology, 030304 developmental biology, 0303 health sciences, Behavior, Animal, Mechanism (biology), Host (biology), [SDV.BA]Life Sciences [q-bio]/Animal biology, 030302 biochemistry & molecular biology, fungi, Cell Biology, Honey bee, Bees, Ketones, biology.organism_classification, Brood, Varroa destructor, behavior and behavior mechanisms, Varroa, Biological Assay, Female
Abstract

International audience; Invasive species events related to globalization are increasing, resulting in parasitic outbreaks. Understanding of host defense mechanisms is needed to predict and mitigate against the consequences of parasite invasion. Using the honey bee Apis mellifera and the mite Varroa destructor, as a host–parasite model, we provide a comprehensive study of a mechanism of parasite detection that triggers a behavioral defense associated with social immunity. Six Varroa-parasitization-specific (VPS) compounds are identified that trigger Varroa-sensitive hygiene (VSH, bees’ key defense against Varroa sp.), enable the selective recognition of a parasitized brood and induce responses that mimic intrinsic VSH activity in bee colonies. We also show that individuals engaged in VSH exhibit a unique ability to discriminate VPS compounds from healthy brood signals. These findings enhance our understanding of a critical mechanism of host defense against parasites, and have the potential to apply the integration of pest management in the beekeeping sector.

Published
2021
Full Text
View/download PDF

7. A combined LC-MS and NMR approach to reveal metabolic changes in the hemolymph of honeybees infected by the gut parasite Nosema ceranae

Author

Cyril Vidau, Marie Diogon, Angélique Abila, Bernard Lyan, Hicham El Alaoui, Frédéric Delbac, Céline Dalle, Mounir Traïkia, Cyril Jousse, Institut de Chimie de Clermont-Ferrand (ICCF), SIGMA Clermont (SIGMA Clermont)-Institut de Chimie du CNRS (INC)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), MetaboHUB-Clermont, MetaboHUB, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut de l'abeille (ITSAP), Abeilles et Environnement (AE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR-12-BSV3-0020,BEELOSS,Analyse de la synergie parasite/insecticide dans la perte des colonies d'abeilles à l'aide d'approches complémentaires chez Apis mellifera et l'organisme modèle Drosophila melanogaster(2012), MetaboHUB, Plateforme d’Exploration du Métabolisme, Université Clermont Auvergne, Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille), and UMT PrADE

Subjects
0106 biological sciences, 0301 basic medicine, Magnetic Resonance Spectroscopy, Metabolite, Honeybee, Metabolomic, Biology, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, Nosema, Tandem Mass Spectrometry, Hemolymph, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Pathogen, Ecology, Evolution, Behavior and Systematics, Chromatography, High Pressure Liquid, Intracellular parasite, [SDV.BA]Life Sciences [q-bio]/Animal biology, Lipid metabolism, Bees, biology.organism_classification, Nosema ceranae, 3. Good health, Spermidine, 010602 entomology, 030104 developmental biology, chemistry, Biochemistry, Stress biomarkers, Host-Pathogen Interactions, Metabolome, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience; Nosema ceranae is an emerging and invasive gut pathogen in Apis mellifera and is considered as a factor contributing to the decline of honeybee populations. Here, we used a combined LC-MS and NMR approach to reveal the metabolomics changes in the hemolymph of honeybees infected by this obligate intracellular parasite. For metabolic profiling, hemolymph samples were collected from both uninfected and N. ceranae-infected bees at two time points, 2 days and 10 days after the experimental infection of emergent bees. Hemolymph samples were individually analyzed by LC-MS, whereas each NMR spectrum was obtained from a pool of three hemolymphs. Multivariate statistical PLS-DA models clearly showed that the age of bees was the parameter with the strongest effect on the metabolite profiles. Interestingly, a total of 15 biomarkers were accurately identified and were assigned as candidate biomarkers representative of infection alone or combined effect of age and infection. These biomarkers included carbohydrates (α/β glucose, α/β fructose and hexosamine), amino acids (histidine and proline), dipeptides (Glu-Thr, Cys-Cys and γ-Glu-Leu/Ile), metabolites involved in lipid metabolism (choline, glycerophosphocholine and O-phosphorylethanolamine) and a polyamine compound (spermidine). Our study demonstrated that this untargeted metabolomics-based approach may be useful for a better understanding of pathophysiological mechanisms of the honeybee infection by N. ceranae.

Published
2020
Full Text
View/download PDF

8. Autosomal and Mitochondrial Adaptation Following Admixture: A Case Study on the Honeybees of Reunion Island

Author

Wragg, David, Técher, Maéva Angélique, Canale-Tabet, Kamila, Basso, Benjamin, Bidanel, Jean Pierre, Labarthe, Emmanuelle, Bouchez, Olivier, Le Conte, Yves, Clémencet, Johanna, Delatte, Hélène, Vignal, Alain, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Université Fédérale Toulouse Midi-Pyrénées, Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université de La Réunion (UR), Université de La Réunion (UR), Ecology and Evolution Unit, Okinawa Institute of Science and Technology (OIST), Roslin Institute, University of Edinburgh, UMT Prade, Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille), Association de Coordination Technique Agricole (ACTA), Association pour le Développement de l'Apiculture Provençale (ADAPI), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, GeT PlaGe, Genotoul, Institut National de la Recherche Agronomique (INRA), Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Okinawa Institute of Science and Technology Graduate University (OIST), UR 406 Abeilles & Environnement, France AgriMer, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT), Université de Toulouse (UT), The Roslin Institute, Biotechnology and Biological Sciences Research Council (BBSRC), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Les instituts techniques agricoles (Acta), Association pour le Developpement de l'Apiculture Provencale (ADAPI), Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), GenPhySE - UMR 1388 ( Génétique Physiologie et Systèmes d'Elevage ), Institut National de la Recherche Agronomique ( INRA ) -École nationale supérieure agronomique de Toulouse [ENSAT]-ENVT, Institut National Polytechnique de Toulouse ( INPT ), Université de Toulouse, UMR PVBMT, Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ), Université de la Réunion ( UR ), Institut Technique et Scientifique de l'Apiculture et de la Pollinisation ( ITSAP-Institut de l'Abeille ), Association de Coordination Technique Agricole, Association pour le Développement de l'Apiculture Provençale ( ADAPI ), Génétique Animale et Biologie Intégrative ( GABI ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Université Paris Saclay, Institut National de la Recherche Agronomique ( INRA ), Abeilles et Environnement ( AE ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Avignon et des Pays de Vaucluse ( UAPV ), and Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3)

Subjects
Male, Acclimatization, [SDV]Life Sciences [q-bio], Genome, Insect, Évolution, adaptation, Apis mellifera mellifera, Génétique des populations, Apis mellifera scutellata, Marqueur génétique, insects, Introduction d'animaux, Genomics, Bees, Mitochondrie, Adaptation, Physiological, Mitochondria, [SDE]Environmental Sciences, Female, Apis mellifera, Apis mellifera ligustica, Research Article, Zone tropicale, Apis mellifera carnica, Séquence nucléotidique, DNA, Mitochondrial, Molecular Evolution, genomics/proteomics, Genetics, Animals, [INFO]Computer Science [cs], Adaptation, insect, molecular evolution, [SDV.GEN]Life Sciences [q-bio]/Genetics, Génome, [ SDV ] Life Sciences [q-bio], L60 - Taxonomie et géographie animales, L10 - Génétique et amélioration des animaux, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, Genome, Mitochondrial, Reunion
Abstract

The honeybee population of the tropical Reunion Island is a genetic admixture of the Apis mellifera unicolor subspecies, originally described in Madagascar, and of European subspecies, mainly A. m. carnica and A. m. ligustica , regularly imported to the island since the late 19th century. We took advantage of this population to study genetic admixing of the tropical-adapted indigenous and temperate-adapted European genetic backgrounds. Whole genome sequencing of 30 workers and 6 males from Reunion, compared with samples from Europe, Madagascar, Mauritius, Rodrigues, and the Seychelles, revealed the Reunion honeybee population to be composed on an average of 53.2 6 5.9% A. m. unicolor nuclear genomic background, the rest being mainly composed of A. m. carnica andtoalesserextent A. m. ligustica . In striking contrast to this, only 1 out of the 36 honeybees from Reunion had a mitochondrial genome of European origin, suggesting selection has favored the A. m. unicolor mitotype, which is possibly better adapted to the island’s bioclimate. Local ancestry was determined along the chromosomes for all Reunion samples, and a test for preferential selection for the A. m. unicolor or European background revealed 15 regions significantly associated with the A. m. unicolor lineage and 9 regions with the European lineage. Our results provide insights into the long- term consequences of introducing exotic specimen on the nuclear and mitochondrial genomes of locally adapted populations

Published
2017
Full Text
View/download PDF

9. Ruthenium complexes bearing amino-bis(phosphinite) or amino-bis(aminophosphine) ligands: Application in catalytic ester hydrogenation

Author

Jean-Baptiste Sortais, Jean-Luc Couturier, Jean-Luc Dubois, Thierry Roisnel, Christophe Darcel, Jean-François Carpentier, Mickaël Henrion, Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des Abeilles dans l'Environnement, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche Rhône-Alpes (CRRA), Arkema (Arkema), Arkema, Colombes, European Commission [635405], UR 406 Abeilles & Environnement, Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Abeilles et environnement (AE), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)

Subjects
inorganic chemicals, Phosphinite, Dimer, chemistry.chemical_element, Noyori asymmetric hydrogenation, Homogeneous catalysis, 010402 general chemistry, 01 natural sciences, Medicinal chemistry, Catalysis, chemistry.chemical_compound, ester hydrogenation, Organic chemistry, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, ruthenium, Ester derivatives, 010405 organic chemistry, Process Chemistry and Technology, Cationic polymerization, homogeneous catalysis, 0104 chemical sciences, Ruthenium, chemistry, pincer complexes
Abstract

International audience; Mononuclear hydrido and dinuclear chloro ruthenium(II) complexes derived from readily available amino-bis(phosphinite) and amino(bisaminophosphine) tridentate ligands were applied in the catalytic hydrogenation of ester derivatives. Notably, the cationic ruthenium dimer 2c proved to be an efficient pre-catalyst when used in combination with (KOBu)-Bu-t in THF at 120 degrees C and 50 bar H-2 with aromatic and aliphatic esters. (C) 2017 Elsevier B.V. All rights reserved.

Published
2017
Full Text
View/download PDF

10. The bee fauna of large parks in downtown Paris, France

Author

Isabelle Dajoz, Michael Kuhlmann, Violette Le Féon, Benoît Geslin, Bernard E. Vaissière, Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Institut d'écologie et des sciences de l'environnement de Paris (IEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), UMT Protection des Abeilles dans l'Environnement, UMR 406 Abeilles et environnement, Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), UMT Protection des abeilles dans l’environnement (UMT PrADE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille)-Centre National de la Recherche Scientifique (CNRS)-Association pour le Développement de l'Apiculture Provençale (ADAPI)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Abeilles et environnement (AE), Institut National de la Recherche Agronomique (INRA), Region Ile-de-France through DIM Astrea [ASTREA 2009-01-22], R2DS programs, and Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0106 biological sciences, Halictidae, biology, Pollination, Ecology, Fauna, Biodiversity, 15. Life on land, biology.organism_classification, dominance, 010603 evolutionary biology, 01 natural sciences, Lasioglossum, 010602 entomology, wild bee assemblage, Urban ecology, urban ecology, Insect Science, Dominance (ecology), functional traits, Urban ecosystem, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Ecology, Evolution, Behavior and Systematics
Abstract

International audience; In recent years, a growing number of studies have focused on the potential interest of urban green areas for supporting biodiversity. Private gardens, urban parks or green roofs may support relatively high densities of diverse wild bees. Knowledge is lacking regarding bee assemblages in Paris, the French capital, and one of the most densely populated part of France. In this context, we here provide a first assessment of the taxonomic and functional composition of bee assemblages in three urban parks in downtown Paris. Bees were sampled with a set of three coloured pan traps per park. Fifteen 24-hour sessions were performed from April to July 2011. We captured 425 specimens from 44 species representing five families and 11 genera. The assemblage was dominated by Halictidae (15 species representing 70.1% of total abundance), especially the eusocial species Lasioglossum morio that made 25.2% of total abundance. From a functional point of view, there were only two species of parasitic bees that made 1.2% of total abundance. Most non-parasitic species were polylectic and below-ground nesting. This study highlights the importance of preserving and managing urban parks and other green areas to promote the conservation of wild bees and ultimately the functionality of pollination service in urban ecosystems.

Published
2015
Full Text
View/download PDF

11. Conventional versus stealth lipid nanoparticles: formulation and in vivo fate prediction through FRET monitoring

Author

Isabelle Texier, Elisabetta Pancani, Jean-Luc Coll, Margaux Wiber, Jérôme Bejaud, Anne-Laure Lainé, Lucie Sancey, M. Henry, Julien Gravier, Jean-Pierre Benoit, Catherine Passirani, Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT Protection des Abeilles dans l'Environnement, Univ Angers, Okina, and Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Biodistribution, Fluorescence-lifetime imaging microscopy, [SDV]Life Sciences [q-bio], Mice, Nude, Pharmaceutical Science, Nanotechnology, Fluorescence imaging, Polyethylene Glycols, Nanocapsules, In vivo, PEG ratio, Fluorescence Resonance Energy Transfer, Animals, Tissue Distribution, Fluorescent Dyes, Drug Carriers, Chemistry, Optical Imaging, Drug release, Lipids, [SDV] Life Sciences [q-bio], Encapsulation stability, Förster resonance energy transfer, Nanomedicine, Biophysics, PEGylation, Female, Nanocarriers
Abstract

International audience; The determination of the nanocarrier fate in preclinical models is required before any translation from laboratory to clinical trials. Modern fluorescent imaging techniques have gained considerable advances becoming a powerful technology for non-invasive visualization in living subjects. Among them, Forster (fluorescence) resonance energy transfer (FRET) is a particular fluorescence imaging which involves energy transfer between 2 fluorophores in a distance-dependent manner. Considering this feature, the encapsulation of an acceptor/donor pair in lipid nanoparticles (LNEs: lipid nanoemulsions, LNCs: lipid nanocapsules) allowed the carrier integrity to be tracked. Accordingly, we used this FRET technique to evaluate the behavior of LNEs, conventional LNCs and newly designed stealth LNCs. After the development through a one-step (OS) PEGylation process of these stealth LNCs (OS LNCs), in vitro guest exchange dynamics and release kinetics were evaluated for both LNC formulations. We thereafter assessed in vivo biodistribution of all types of lipid nanoparticles. Results showed enhanced stability of encapsulation in OS LNCs in comparison to conventional LNCs. Additionally, the presence of the long PEG chains on the lipid nanoparticle surface altered the biodistribution pattern. Despite different release kinetic profiles, OS LNCs and LNEs showed extended blood circulation time associated with a good structure stability over several hours after intravenous injection.

Published
2014
Full Text
View/download PDF

12. Pesticide risk assessment in free-ranging bees is weather and landscape dependent

Author

Pierrick Aupinel, Colette Bertrand, Vincent Bretagnolle, Violette Le Féon, Mickaël Henry, Jean-François Odoux, Axel Decourtye, Fabrice Requier, Henry, Mickaël, UMT Protection des Abeilles dans l'Environnement, Abeilles et environnement (AE), Institut National de la Recherche Agronomique (INRA), SAD Paysage (SAD Paysage), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Entomologie (ENTOMOLOGIE), ITSAP-Institut de l'Abeille, ITSAP, Association de Coordination Technique Agricole (ACTA), French Ministry of Agriculture (TECHBEE and RISQAPI projects), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA), UMR 406 Abeilles et environnement, Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), SAD Paysages, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), UE Entomologie (INRA), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Institut de l'abeille (ITSAP), and Les instituts techniques agricoles (Acta)

Subjects
toxicité letale, General Physics and Astronomy, Context (language use), Biology, Risk Assessment, General Biochemistry, Genetics and Molecular Biology, Sciences de l'environnement, Homing Behavior, Animals, Pesticides, Weather, abeille, Field exposure, insecte pollinisateur, Multidisciplinary, Behavior, Animal, Dose-Response Relationship, Drug, Geography, Free ranging, business.industry, Ecology, Environmental resource management, General Chemistry, Bees, Pesticide risk assessment, produit phytosanitaire, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business, Risk assessment, contexte paysager, Environmental Sciences
Abstract

Zone Atelier Plaine & Val de Sèvre; International audience; The risk assessment of plant protection products on pollinators is currently based on the evaluation of lethal doses through repeatable lethal toxicity laboratory trials. Recent advances in honeybee toxicology have, however, raised interest on assessing sublethal effects in free-ranging individuals. Here, we show that the sublethal effects of a neonicotinoid pesticide are modified in magnitude by environmental interactions specific to the landscape and time of exposure events. Field sublethal assessment is therefore context dependent and should be addressed in a temporally and spatially explicit way, especially regarding weather and landscape physiognomy. We further develop an analytical Effective Dose (ED) framework to help disentangle context-induced from treatment-induced effects and thus to alleviate uncertainty in field studies. Although the ED framework involves trials at concentrations above the expected field exposure levels, it allows to explicitly delineating the climatic and landscape contexts that should be targeted for in-depth higher tier risk assessment.

Published
2014
Full Text
View/download PDF

13. Influence of pollen nutrition on honey bee health: do pollen quality and diversity matter?

Author

André Kretzschmar, Marion Salignon, Luc P. Belzunces, Garance Di Pasquale, Yves Le Conte, Cédric Alaux, Axel Decourtye, Séverine Suchail, Jean Luc Brunet, UMT Protection des Abeilles dans l'Environnement, Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Biostatistique et Processus Spatiaux (BioSP), Institut National de la Recherche Agronomique (INRA), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), European Project: 797/2004, UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), and Alaux, Cédric

Subjects
0106 biological sciences, Pollen source, Survival, Science, Foraging, Context (language use), medicine.disease_cause, 010603 evolutionary biology, 01 natural sciences, complex mixtures, Honey bees, Nosema, Parasite physiology, Pollen, Botany, medicine, Animals, 2. Zero hunger, Multidisciplinary, biology, fungi, food and beverages, Honey bee, 15. Life on land, Bees, biology.organism_classification, Lipids, Parasitic diseases, Nosema ceranae, Animal Feed, Diet, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Worker bee, 010602 entomology, behavior and behavior mechanisms, Medicine, Animal Nutritional Physiological Phenomena, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Research Article
Abstract

International audience; Honey bee colonies are highly dependent upon the availability of floral resources from which they get the nutrients (notably pollen) necessary to their development and survival. However, foraging areas are currently affected by the intensification of agriculture and landscape alteration. Bees are therefore confronted to disparities in time and space of floral resource abundance, type and diversity, which might provide inadequate nutrition and endanger colonies. The beneficial influence of pollen availability on bee health is well-established but whether quality and diversity of pollen diets can modify bee health remains largely unknown. We therefore tested the influence of pollen diet quality (different monofloral pollens) and diversity (polyfloral pollen diet) on the physiology of young nurse bees, which have a distinct nutritional physiology (e.g. hypopharyngeal gland development and vitellogenin level), and on the tolerance to the microsporidian parasite Nosema ceranae by measuring bee survival and the activity of different enzymes potentially involved in bee health and defense response (glutathione-S-transferase (detoxification), phenoloxidase (immunity) and alkaline phosphatase (metabolism)). We found that both nurse bee physiology and the tolerance to the parasite were affected by pollen quality. Pollen diet diversity had no effect on the nurse bee physiology and the survival of healthy bees. However, when parasitized, bees fed with the polyfloral blend lived longer than bees fed with monofloral pollens, excepted for the protein-richest monofloral pollen. Furthermore, the survival was positively correlated to alkaline phosphatase activity in healthy bees and to phenoloxydase activities in infected bees. Our results support the idea that both the quality and diversity (in a specific context) of pollen can shape bee physiology and might help to better understand the influence of agriculture and land-use intensification on bee nutrition and health.

Published
2013
Full Text
View/download PDF

14. A Locomotor Deficit Induced by Sublethal Doses of Pyrethroid and Neonicotinoid Insecticides in the Honeybee Apis mellifera

Author

Mickaël Henry, Guy Rodet, Pierre Charnet, Jean-Christophe Sandoz, Axel Decourtye, Mercedes Charreton, Claude Collet, Abeilles & Environnement (UR 406 ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UMT PrADE, Institut Technique et Scientifique de l'Apiculture et de la Pollinisation (ITSAP-Institut de l'Abeille), Association de Coordination Technique Agricole (ACTA), Évolution, génomes, comportement et écologie (EGCE), Université Paris-Sud - Paris 11 (UP11)-IRD-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche, France Agrimer, Centre National de la Recherche Scientifique (CNRS)-IRD-Université Paris-Sud - Paris 11 (UP11), Abeilles et environnement (AE), Institut National de la Recherche Agronomique (INRA), UMT Protection des abeilles dans l’environnement (UMT PrADE), Association pour le Developpement de l'Apiculture Provencale (ADAPI)-Institut de l'abeille (ITSAP)-Centre National de la Recherche Scientifique (CNRS)-Terres Inovia-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de l'abeille (ITSAP), Les instituts techniques agricoles (Acta), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Collet, Claude

Subjects
Insecticides, [SDV]Life Sciences [q-bio], lcsh:Medicine, Motor Activity, honeybee, Biology, influence de l'exposition, Cypermethrin, Lethal Dose 50, Toxicology, Neonicotinoids, chemistry.chemical_compound, apis mellifera, pyrethrinoide, Nitriles, Oxazines, Pyrethrins, Animals, lcsh:Science, Guanidine, Fipronil, Multidisciplinary, Pyrethroid, insecticide neonicoitnoide, lcsh:R, Neonicotinoid, Bees, Pesticide, Nitro Compounds, Brood, dose sublétale, Thiazoles, chemistry, durée d'exposition, test locomoteur, Pyrazoles, lcsh:Q, Thiamethoxam, mortalité, Tetramethrin, Research Article
Abstract

International audience; The toxicity of pesticides used in agriculture towards non-targeted organisms and especially pollinators has recently drawn the attention from a broad scientific community. Increased honeybee mortality observed worldwide certainly contributes to this interest. The potential role of several neurotoxic insecticides in triggering or potentiating honeybee mortality was considered, in particular phenylpyrazoles and neonicotinoids, given that they are widely used and highly toxic for insects. Along with their ability to kill insects at lethal doses, they can compromise survival at sublethal doses by producing subtle deleterious effects. In this study, we compared the bee's locomotor ability, which is crucial for many tasks within the hive (e.g. cleaning brood cells, feeding larvae…), before and after an acute sublethal exposure to one insecticide belonging to the two insecticide classes, fipronil and thiamethoxam. Additionally, we examined the locomotor ability after exposure to pyrethroids, an older chemical insecticide class still widely used and known to be highly toxic to bees as well. Our study focused on young bees (day 1 after emergence) since (i) few studies are available on locomotion at this stage and (ii) in recent years, pesticides have been reported to accumulate in different hive matrices, where young bees undergo their early development. At sublethal doses (SLD48h, i.e. causing no mortality at 48h), three pyrethroids, namely cypermethrin (2.5 ng/bee), tetramethrin (70 ng/bee), tau-fluvalinate (33 ng/bee) and the neonicotinoid thiamethoxam (3.8 ng/bee) caused a locomotor deficit in honeybees. While the SLD48h of fipronil (a phenylpyrazole, 0.5 ng/bee) had no measurable effect on locomotion, we observed high mortality several days after exposure, an effect that was not observed with the other insecticides. Although locomotor deficits observed in the sublethal range of pyrethroids and thiamethoxam would suggest deleterious effects in the field, the case of fipronil demonstrates that toxicity evaluation requires information on multiple endpoints (e.g. long term survival) to fully address pesticides risks for honeybees. Pyrethroid-induced locomotor deficits are discussed in light of recent advances regarding their mode of action on honeybee ion channels and current structure-function studies.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results