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Mosquitoes are carriers of diseases like malaria, dengue fever, chikungunya, zika fever, and lymphatic filariasis, which can be deadly in Tropical Africa and certain subtropical regions. The World Health Organization recognizes mosquito bites as the primary cause of over a million deaths annually due to malaria. It is crucial to reduce or eliminate the mosquito population in Africa if possible. Presently, malaria vector control methods mainly involve using insecticide-treated bed nets, long-lasting insecticidal nets, or indoor residual spraying. However, many existing mosquitos control products have not proven to be effective in eradicating mosquitoes in these regions. In this study, a solar-powered mosquito killer with voice activation was developed using an Arduino board, an ultrasonic sensor, an electric fence driver, a direct current (DC) fan, and UV lights. The Ultraviolet (UV) lights are visible to some insects and enhance the trapping effect by combining heat and carbon dioxide. The device has voice activation to minimize human contact. It is an improvement over previous methods that utilized voiceactivated equipment without harmful elements or irritating insecticides. The device mimics a mosquito's sensory receptors to attract and ultimately eliminate them. The equipment includes a 5V DC fan operating at 1800-3700 rev/min, a mist humidifier emitting mist at regular intervals (operating frequency of 0.8MHz - 1.75MHz), an electric fence producing over 1000 volts to render mosquitoes lifeless upon contact, and a voice module for device control. The device is powered by an external outlet power cord supplying 5-12 volts to activate the micro-controller. [ABSTRACT FROM AUTHOR]

A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased assessment of the influence of environmental factors at the single cell level. First, based on the measurement of intracellular homeostasis of reactive oxygen species (ROS) with a DCFH-DA dye, the effects of various external stress factors such as H2O2, temperature, ultraviolet (UV) light, and cadmium ions on intracellular ROS accumulation in Arabidopsis mesophyll protoplasts were quantitatively investigated. Second, a faster and stronger oxidative burst was observed in Petunia protoplasts isolated from white petals than in those isolated from purple petals, demonstrating the photoprotective role of anthocyanins. Third, using mutants with different endogenous auxin, we demonstrated the beneficial effect of auxin during the process of primary cell wall regeneration. Moreover, UV-B irradiation has a similar accelerating effect by increasing the intracellular auxin level, as shown by double fluorescence channels. In summary, our work has revealed previously underappreciated phenotypic variability within a protoplast population and demonstrated the advantages of a microfluidic flow cytometry for assessing the in vivo dynamics of plant metabolic and physiological indices at the single-cell level. [ABSTRACT FROM AUTHOR]

A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased assessment of the influence of environmental factors at the single cell level. First, based on the measurement of intracellular homeostasis of reactive oxygen species (ROS) with a DCFH-DA dye, the effects of various external stress factors such as H2O2, temperature, ultraviolet (UV) light, and cadmium ions on intracellular ROS accumulation in Arabidopsis mesophyll protoplasts were quantitatively investigated. Second, a faster and stronger oxidative burst was observed in Petunia protoplasts isolated from white petals than in those isolated from purple petals, demonstrating the photoprotective role of anthocyanins. Third, using mutants with different endogenous auxin, we demonstrated the beneficial effect of auxin during the process of primary cell wall regeneration. Moreover, UV-B irradiation has a similar accelerating effect by increasing the intracellular auxin level, as shown by double fluorescence channels. In summary, our work has revealed previously underappreciated phenotypic variability within a protoplast population and demonstrated the advantages of a microfluidic flow cytometry for assessing the in vivo dynamics of plant metabolic and physiological indices at the single-cell level.

The current literature lacks a comprehensive discussion on the trade-off between pollutant degradation/mineralization and treatment time costs in utilizing UV light in combination with H 2 O 2 -based electrochemical advanced oxidation processes (EAOPs). The present study sheds light on the benefits of using the photoelectro-Fenton (PEF) process with UVA or UVC for methylparaben (MetP) degradation in real drinking water. Although light boosts the photodegradation of refractory Fe(III) complexes and the photolysis of H 2 O 2 (with UVC only), the energy-intensive nature of light-based treatments is acknowledged. To help tackle the high energy consumption issue, a novel approach was employed: partial application of UVA or UVC light after a predetermined electro-Fenton electrolysis time. The proposed treatment approach yielded satisfactory comparable results to those obtained from the application of PEF/UVA or PEF/UVC in terms of total organic carbon removal (ca. 100%), with notably lower energy consumption (ca. 50%). The study delves into the combined method's feasibility, analyzing pollutant degradation/mineralization process and overall energy consumption. The research identifies possible degradation routes based on intermediate detection and radical quenching experiments. Finally, toxicological assessments evaluate the toxicity levels of MetP and its intermediates. The findings of this study bring meaningful contributions to the fore and point to the highly promising potential of the proposed approach, in terms of sustainability and cost-effectiveness, when applied for decentralized water treatment. [Display omitted] • Combining H 2 O 2 -based electrochemical advanced oxidation processes with UV light for the oxidation of pollutants. • EF + UVC system can outperform in 50% the PEF process in terms of specific energy consumption per TOC. • A possible intermediate route is proposed; most intermediates and by-products have not harmful toxicity. • A sustainable cost-effective decentralized system for water treatment was obtained. [ABSTRACT FROM AUTHOR]

Many New Zealand residential homes suffer from poor indoor air quality (IAQ). Cold, damp and mould-ridden homes can cause severe respiratory health problems. Poor IAQ can arise due to inadequate insulation and ventilation and is compounded when dwellers cannot afford to heat their homes during colder months. Grandfathering of standards means many New Zealand houses have single glazed windows and insufficient or no wall insulation. These have become mandatory for newly built houses since 2016 under the New Zealand Standard NZS4246:2016 Energy Efficiency. This paper aims to present the results of modified ultraviolet germicidal irradiation (UVGI) lights-dehumidifier coupled with two different types of filters (carbon-fibre or polyester). The modified devices were designed to improve ambient air temperature by removing moisture, particulate matter and reducing mould and bacteria growth in a room. Seven different scenarios were investigated, which include combinations of UV light and filtration. The results showed that the UVGI was superior to filtration only; however, the polyester filter with UV lights (PFUV) was the most effective configuration. The PFUV effectively impeded the mould growth, with a 13.3% reduction in relative humidity and a 4.1 °C increase in the room temperature in the absence of a heating source. The results showed good potential for improving the IAQ of buildings. [ABSTRACT FROM AUTHOR]