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14 results on '"Uchea, Chibuzor"'

2. Optimising antimicrobial use in humans – review of current evidence and an interdisciplinary consensus on key priorities for research

Author

Charani, Esmita, McKee, Martin, Ahmad, Raheelah, Balasegaram, Manica, Bonaconsa, Candice, Merrett, Gemma Buckland, Busse, Reinhard, Carter, Vanessa, Castro-Sanchez, Enrique, Franklin, Bryony D, Georgiou, Pantelis, Hill-Cawthorne, Kerri, Hope, William, Imanaka, Yuichi, Kambugu, Andrew, Leather, Andrew JM, Mbamalu, Oluchi, McLeod, M, Mendelson, Marc, Mpundu, Mirfin, Rawson, Timothy M, Ricciardi, Walter, Rodriguez-Manzano, Jesus, Singh, Sanjeev, Tsioutis, Constantinos, Uchea, Chibuzor, Zhu, Nina, and Holmes, Alison H

Published
2021
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3. The utility of trout hepatic cells in the prediction of xenobiotic bioaccumulation and environmental persistence

Author

Uchea, Chibuzor

Subjects
500, QL Zoology, QR Microbiology
Abstract

The need to protect aquatic organisms from the toxic potential of environmental contaminants with minimal use of animal testing has been highlighted by the introduction of a number of regulatory directives. This thesis proposes the potential of 3D cell culture models to ‘bridge the gap’ between the use of existing subcellular and in vivo systems for the testing of compounds for potential persistence, bioaccumulation and toxicity, improving the predictive power of in vitro screening procedures. In this project, a trout hepatocyte spheroid model was optimised and using metabolic, fluorescence, transport and gene expression assays, the utility of this system in such studies as a superior alternative to currently used in vitro models was demonstrated. Hepatocytes cultured as spheroids remained viable for up to 40 days, exhibiting significantly greater functional xenobiotic metabolism and transport, as well as expression of genes, compared to other in vitro systems. These features of spheroids present a profile more closely representative of that of the whole liver. Together with the potential advantages of screening highly persistent compounds that require long term incubation and possible use in the assessment of the toxicity of compounds exhibiting chronic effects, this system successfully enriches current in vitro testing strategies.

Published
2013

4. Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples

Author

Yin, Xiaole, Chen, Xi, Jiang, Xiao-Tao, Yang, Ying, Li, Bing, Shum, Marcus Ho-Hin, Lam, Tommy T Y, Leung, Gabriel M, Rose, Joan, Sanchez-Cid, Concepcion, Vogel, Timothy M, Walsh, Fiona, Berendonk, Thomas U, Midega, Janet, Uchea, Chibuzor, Frigon, Dominic, Wright, Gerard D, Bezuidenhout, Carlos, Picão, Renata C, Ahammad, Shaikh Z, Nielsen, Per Halkjær, Hugenholtz, Philip, Ashbolt, Nicholas J, Corno, Gianluca, Fatta-Kassinos, Despo, Bürgmann, Helmut, Schmitt, Heike, Cha, Chang-Jun, Pruden, Amy, Smalla, Kornelia, Cytryn, Eddie, Zhang, Yu, Yang, Min, Zhu, Yong-Guan, Dechesne, Arnaud, Smets, Barth F, Graham, David W, Gillings, Michael R, Gaze, William H, Manaia, Célia M, van Loosdrecht, Mark C M, Alvarez, Pedro J J, Blaser, Martin J, Tiedje, James M, Topp, Edward, and Zhang, Tong

Subjects
standardization, metagenomics, one-health, long-read sequencing, ARG copy per cell
Published
2023

8. List of Contributors

Author

Anadón, Arturo, primary, Anderson, Jaime, additional, Anzai, Jun-ichi, additional, Aschner, Michael, additional, Avdonin, Pavel, additional, Bajgar, Jiri, additional, Bakshi, Kulbir, additional, Balali-Mood, Mahdi, additional, Balszuweit, Frank, additional, Banerjee, Atrayee, additional, Bast, Cheryl B., additional, Bhattacharya, Rahul, additional, Bollinger, Claire E., additional, Casillas, Robert P., additional, Chemtob, Sylvain, additional, Clark, Ryan, additional, Clarkson, Edward D., additional, Cole, Toby B., additional, Coppock, Robert W., additional, Costa, Lucio G., additional, Dettbarn, Wolf-D., additional, Dobbins, Dorothy L., additional, Dorsey, Russell, additional, Dziwenka, Margitta, additional, Emmett, George, additional, Estévez, Jorge, additional, Evans, Timothy J., additional, Fink, John K., additional, Flora, Swaran J.S., additional, Fonnum, Frode, additional, Furlong, Clement E., additional, Fusek, Josef, additional, Gearhart, Jeffery M., additional, Gerecke, Donald R., additional, Glass-Mattie, Dana F., additional, Goel, Saryu, additional, Goncharov, Nikolay, additional, Gordon, Richard K., additional, Gray, Joshua P., additional, Grubic, Zoran, additional, Gulati, Kavita, additional, Gupta, Ramesh C., additional, Gwaltney-Brant, Sharon M., additional, Hamilton, Tracey L., additional, Hauschild, Veronique, additional, Hein, Nichole D., additional, Hilmas, Corey J., additional, Hilmas, Elora, additional, Hood, Darryl B., additional, Jakubowski, Edward M., additional, Jenkins, Richard O., additional, Jett, David A., additional, Jiang, George C-T, additional, Jiao, Yuqin, additional, John, Harald, additional, Johnson, Nathan H., additional, Jokanović, Milan, additional, Jun, Daniel, additional, Kadakkuzha, Beena M., additional, Kassa, Jiri, additional, Katalinic, Maja, additional, Kehe, Kai, additional, Khlebnikova, Natalia, additional, King, Joseph, additional, Kodavanti, Urmila P., additional, Korabecny, Jan, additional, Krishna, Gopala, additional, Krivorotova, Nadezhda, additional, Kuca, Kamil, additional, Kuroiwa, Yukio, additional, Kuznetsov, Anatoliy, additional, Kuznetsov, Sergey, additional, Larsen, Joseph C., additional, Leung, Yiuka, additional, Liu, Jing, additional, Liu, Xin-an, additional, Lockridge, Oksana, additional, Loganathan, Bommanna G., additional, Lugo, Andres M., additional, Maguire, Mark, additional, Makhaeva, Galina F., additional, Malik, Jitendra K., additional, Mars, Tomaz, additional, Martínez-Larrañaga, Maria Rosa, additional, Masson, Patrick, additional, Masunaga, Shigeki, additional, McCallister, Monique, additional, McCauley, Linda A., additional, McNutt, Patrick M., additional, Meek, Edward, additional, Merrill, Elaine, additional, Milanez, Sylvia, additional, Mindukshev, Igor, additional, Mis, Katarina, additional, Mulay, Shree, additional, Murphy, Michael J., additional, Musilek, Kamil, additional, Myhrer, Trond, additional, Okumura, Tetsu, additional, Opresko, Dennis, additional, Orlova, Tatiana, additional, Pål, Aas, additional, Pan, Xiaoping, additional, Patocka, Jiri, additional, Pirkmajer, Sergej, additional, Pita, Rene, additional, Pitt, Jason, additional, Pope, Carey, additional, Prokofieva, Daria, additional, Radilov, Andrey, additional, Ramaiah, Shashi K., additional, Ramesh, Aramandla, additional, Ray, Arunabha, additional, Ray, Kausik, additional, Rembovskiy, Vladimir, additional, Rhoades, Raina, additional, Richardson, Rudy J., additional, Rizzo, Valerio, additional, Robinson, Peter J., additional, Ruark, Chris, additional, Salem, Harry, additional, Satoh, Tetsuo, additional, Savage, Russell E., additional, Savelieva, Elena, additional, Schopfer, Lawrence M., additional, Sciuto, Alfred M., additional, Seto, Yasuo, additional, Shakarjian, Michael P., additional, Sogorb, Miguel, additional, Soreq, Hermona, additional, Sterri, Sigrun Hanne, additional, Stick, Melissa, additional, Suzuki, Kouichiro, additional, Taki, Kenji, additional, Thiermann, Horst, additional, Thompson, Larry J., additional, Uchea, Chibuzor, additional, Ukolov, Anton, additional, Valerio, Luis G., additional, Van der Merwe, Deon, additional, van der Schans, Marcel J., additional, Varma, Daya R., additional, Vilanova, Eugenio, additional, Vinokurov, Maxim, additional, Voitenko, Natalia, additional, Waiskopf, Nir, additional, Watson, Annetta, additional, Wijeyesakere, Sanjeeva J., additional, Wismer, Tina, additional, Woltjer, Randall L., additional, Mark Worden, R., additional, Worek, Franz, additional, Wright, Linnzi, additional, Yeung, David T., additional, Yoshida, Takemi, additional, Young, Robert A., additional, Zaja-Milatovic, Snjezana, additional, Zinchenko, Valeriy, additional, and Zoltani, Csaba K., additional

Published
2015
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13. In vitro models of xenobiotic metabolism in trout for use in environmental bioaccumulation studies.

Author

Uchea, Chibuzor, Sarda, Sunil, Schulz-Utermoehl, Timothy, Owen, Stewart, and Chipman, Kevin J.

Subjects
*CYTOCHROME P-450 CYP1A1, *RAINBOW trout, *ENVIRONMENTAL risk assessment, *LIVER cells, *OXIDATION
Abstract

1. In vitro screens are sought as informative, alternatives to the use of animals in vivo and to improve upon the current use of fish liver 9000 g supernatants (S9) in environmental risk assessment. 2. The rates of ethoxyresorufin- O-deethylation (relative to S9 protein) measured under different conditions of culture of rainbow trout hepatocytes were significantly higher than those detected in S9, in the order of freshly isolated hepatocytes > 10-day spheroid cultures > primary hepatocytes in culture > S9. The percentage of conjugated metabolites was also similar between freshly isolated and spheroid cultured hepatocytes (9.9 and 13.5%). 3. The rate of oxidation was enhanced (1.7 fold) when S9 was supplemented with cofactors for phase II conjugation but this was only approximately one tenth of the rate in freshly isolated hepatocytes (7.1 pmol/min/mg S9 protein equivalent). 4. Hepatocytes also hydroxylated ibuprofen, producing two metabolites, in contrast to only one (identified as the 1-hydroxy derivative) using hepatic S9 fractions. 5. Since the bioaccumulation potential of chemicals is often based on un-supplemented S9 in incubations ≥1 h when activity declines, it is recommended that predictability would be greatly improved through the use of hepatocyte spheroids, due to their maintenance of activity and longevity. [ABSTRACT FROM AUTHOR]

Published
2013
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14. Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples.

Author

Yin X, Chen X, Jiang XT, Yang Y, Li B, Shum MH, Lam TTY, Leung GM, Rose J, Sanchez-Cid C, Vogel TM, Walsh F, Berendonk TU, Midega J, Uchea C, Frigon D, Wright GD, Bezuidenhout C, Picão RC, Ahammad SZ, Nielsen PH, Hugenholtz P, Ashbolt NJ, Corno G, Fatta-Kassinos D, Bürgmann H, Schmitt H, Cha CJ, Pruden A, Smalla K, Cytryn E, Zhang Y, Yang M, Zhu YG, Dechesne A, Smets BF, Graham DW, Gillings MR, Gaze WH, Manaia CM, van Loosdrecht MCM, Alvarez PJJ, Blaser MJ, Tiedje JM, Topp E, and Zhang T

Subjects
Animals, Humans, RNA, Ribosomal, 16S genetics, Drug Resistance, Microbial genetics, Metagenomics methods, Anti-Bacterial Agents pharmacology, Genes, Bacterial
Abstract

Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health" framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and approaches in bioinformatic analysis. In this article, we consider the commonly used quantification units (ARG copy per cell, ARG copy per genome, ARG density, ARG copy per 16S rRNA gene, RPKM, coverage, PPM, etc.) for profiling ARGs and suggest a universal unit (ARG copy per cell) for reporting such biological measurements of samples and improving the comparability of different surveillance efforts.

Published
2023
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