1. A novel stem cell type at the basal side of the subventricular zone maintains adult neurogenesis
- Author
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Johannes Beckers, Priti Khatri, Claudia Mandl, Yan Shi, Gabriele Hoelzl-Wenig, Yomn Abdullah, Katja Baur, Udo Schmidt-Edelkraut, Martin Irmler, Anna M. Hagenston, and Francesca Ciccolini
- Subjects
Neurogenesis ,Subventricular zone ,Biology ,Neural Stem Cells ,Notch Signalling ,Olfactory Bulb ,Subventricular Zone ,Biochemistry ,Basal (phylogenetics) ,Mice ,Lateral Ventricles ,Genetics ,medicine ,Animals ,Progenitor cell ,Molecular Biology ,reproductive and urinary physiology ,EMBO27 ,EMBO34 ,Article ,Articles ,neural stem cells ,neurogenesis ,notch signalling ,olfactory bulb ,subventricular zone ,Cell Differentiation ,Nestin ,Neural stem cell ,nervous system diseases ,Cell biology ,Olfactory bulb ,ddc ,medicine.anatomical_structure ,nervous system ,Female ,biological phenomena, cell phenomena, and immunity ,Stem cell - Abstract
According to the current consensus, neural stem cells (NSCs) apically contacting the lateral ventricle generate differentiated progenitors by rare asymmetric divisions or by relocating to the basal side of the ventricular-subventricular zone V-SVZ. Both processes will then ultimately lead to the generation of adult-born olfactory bulb (OB) interneurons. In contrast to this view, we here found that adult-born OB interneurons largely derive from an additional NSC type resident in the basal V-SVZ. Despite being both capable of self-renewal and long-term quiescence, apical and basal NSCs differ in Nestin expression, primary cilia extension and frequency of cell division. The expression of Notch-related genes also differed between the two NSC groups and Notch-activation was greatest in apical NSCs. Apical downregulation of Notch-effector Hes1 decreased Notch activation while increasing proliferation across the niche and neurogenesis from apical NSCs. Underscoring their different roles in neurogenesis, lactation-dependent increase in neurogenesis was paralleled by extra activation of basal but not apical NSCs. Thus, basal NSCs support OB neurogenesis whereas apical NSCs impart Notch-mediated lateral inhibition across the V-SVZ. more...
- Published
- 2021