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Teak tree (Tectona grandis) is a tropical tree whose wood is often used in the furniture industry, while the leaves are still underutilized. Teak leaves possess active compounds with potential to be further explored. On the other hand, currently biodegradable-based packaging is being developed to address environmental problems. For this reason, research was carried out on active edible film made from chitosan with addition of active ingredients from teak leaf extract. This study investigated the physical characteristics (color, tensile strength, elongation, solubility, cell microstructure, water vapor transmission rate) and chemical characteristics (moisture content, functional groups, antioxidant and antimicrobial activity) of active edible film made from chitosan with addition of teak leaf extract. The research consisted of three stages. The first stage included the extraction of teak leaf using ethanol and evaluation of its antioxidant and antimicrobial activity. The second stage comprised edible film preparation from chitosan at 1 and 1.5% concentrations, and teak leaf extract addition at 0, 0.1, 0.2% concentrations. The third stage was the characterization of active edible film for physical and chemical properties. The results showed that teak leaf extract had antioxidant activity as evaluated by DPPH method with IC50 value of 46.78 ppm. The teak leave extract also showed antibacterial activity on Staphylococcus aureus. The optimum condition for the production of active edible film was use of 1% chitosan and 0.2% teak leaf extract. The active edible film produced had antioxidant activity of 8.53% RSA, 30.16%, moisture content, 38.28% solubility,1.52 MPa tensile strength, 9.54% elongation, water vapor transmission rate of 3672.32 g/m2, but the film did not show inhibitory effects on S. aureus.

This study analyzed how compensation, organizational culture, and career development the motivation of NCO soldiers in attending Diktukpa through Strategic leadership as a mediating variable. This study addressed the research gap by involving Strategic leadership as a mediating variable to the direct and indirect effects of compensation, organizational culture, and career development on NCO students’ motivation. This quantitative study employed Structural Equation Modeling (SEM) on Amos software 26. Data for this study were obtained from 340 randomly selected NCO students. This research established the existence of a favorable relationship between soldier salary, career advancement, and organizational culture and soldiers’ motivation to participate in Diktukpa as well as strategic leadership. A positive influence was also found in Soldier compensation, career development, and organizational culture on the motivation of Strategic leadership as a mediating variable. The results indicated the direction of evaluation and sound personnel planning that should be taken into account when hiring or accepting officers from non-commissioned officer sources, including pay, career development, organizational culture, and strategic leadership. It is important to boost NCO pupils’ motivation for personal growth. The impact of strategic leadership on soldiers’ motivation is presented in this study in connection to pay and career advancement. . This study provides relevant insights into the current conditions and factors that included compensation and career development, Strategic leadership in influencing the motivation of the soldiers.

Biji Petai (Parkia speciosa Hassk.) merupakan komoditas kacang-kacangan khas Indonesia dengan kandungan protein yang tinggi. Namun, biji Petai juga dikenal mengandung senyawa antigizi asam fitat, tanin dan tripsin inhibitor yang dapat menurunkan nilai cerna protein. Proses pengolahan konvensional kukus selama 10 menit, rebus selama 8 menit, dan goreng selama 2 menit dilakukan untuk menurunkan senyawa antigizi dan meningkatkan kecernaan protein biji Petai. Biji Petai yang digunakan untuk analisis adalah biji Petai bubuk yang telah dikeringkan menggunakan freeze dryer. Efek proses pengolahan divaluasi senyawa gizi, antigizi, dan nilai cerna protein in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh proses pengolahan kukus, rebus, dan goreng terhadap senyawa gizi (kadar air, protein, lemak, abu, dan karbohidrat), antigizi (asam fitat, tanin, serta tripsin inhibitor), dan kecernaan protein in vitro pada Petai. Penelitian dilakukan dengan Rancangan Acak Lengkap Satu Faktor, yaitu jenis proses pengolahan. Data diolah dengan One Way ANOVA menggunakan aplikasi SPSS 2.1 dengan tingkat kepercayaan 95%, kemudian dilanjutkan uji Duncan apabila terdapat beda nyata. Diperoleh hasil bahwa ketiga proses pengolahan tersebut signifikan menurunkan konsentrasi senyawa antigizi asam fitat, tanin, serta tripsin inhibitor. Proses perebusan merupakan proses pengolahan terbaik yang dapat menurunkan senyawa antigizi asam fitat sebesar 75%, tanin sebesar 49%, dan tripsin inhibitor sebesar 70%. Proses pengolahan kukus dan rebus secara signifikan meningkatkan nilai cerna protein in vitro, yaitu masing-masing sebanyak 0,84% dan 2,55%. Temuan ini dapat dijadikan referensi proses pengolahan bagi konsumen biji Petai di Indonesia untuk mendapatkan manfaat asupan protein dari biji Petai dengan maksimal.

Biji kabau merupakan polong-polongan asli Indonesia yang memiliki kandungan protein tinggi. Namun, biji kabau juga memiliki kandungan anti gizi yang tinggi. Oleh karenanya, proses pengolahan perlu dilakukan sebelum biji kabau dikonsumsi untuk menurunkan kandungan senyawa anti gizi di dalamnya. Proses pengolahan tidak hanya mempengaruhi kandungan gizi dan anti gizinya, tetapi juga berpengaruh terhadap karakteristik fisik dan organoleptik biji kabau. Oleh sebab itu, penelitian ini bertujuan untuk mempelajari perubahan fisik dan organoleptik biji kabau yang telah melalui proses pengolahan konvensional, yaitu perebusan, pengukusan dan penggorengan. Biji kabau diperoleh dari Bengkulu. Ruang lingkup pengujian yaitu analisis warna menggunakan chroma meter dan tingkat kekerasan menggunakan universal testing mechine. Uji hedonik dan deskriptif digunakan untuk Analisis Organoleptik. Hasil penelitian menunjukkan bahwa proses pengolahan secara keseluruhan dapat meningkatkan nilai *L pada warna kulit ari biji kabau. Namun, peningkatan durasi pengolahan justru menurunkan nilai *L pada warna endosperma biji kabau. Peningkatan durasi pengolahan juga menurunkan tingkat kekerasan pada daging biji kabau. Berdasarkan parameter kesukaan secara keseluruhan, panelis menyukai biji kabau kukus 10 menit, biji kabau rebus 6 menit, dan biji kabau goreng 3 menit. Hasil penelitian tersebut dapat digunakan sebagai rujukan bagi masyarakat yang ingin mengonsumsi biji kabau agar memiliki karakteristik fisik dan organoleptik yang disukai. Berdasarkan parameter overall panelis menyukai kabau kukus 10 menit, kabau rebus 6 menit, dan kabau goreng 3 menit.

Plant leaves are the primary source of natural colorants for food, mainly due to their chlorophyll content. However, the plant types and the degree of leaf maturity determine the quality and quantity of the chlorophyll. This study aimed to determine the best maturity level of pandan (Pandanus amaryllifolius Roxb.) leaves that serves as potential source of chlorophyll for natural food colorants. Eighty three pandan plants obtained from six different farming locations in Bantul Regency, Yogyakarta, Indonesia were used as samples. The leaves were grouped into four levels of maturity using descriptive statistics based on their morphology, anatomy, color, and chlorophyll contents. The results showed that the average number of leaves ranged from 20-24 leaves per plant (at 95% confidence interval), and 96.4% of the plant had a maximum of 24 leaves. The leaf maturity was grouped into (1) young, (2) medium, (3) mature, and (4) over mature, corresponding to leaf number 1-6, 7-12, 13-18, and 19-24, respectively. The higher the leaf maturity, the higher the chlorophyll content. However, the over mature leaves were only slightly different from the mature ones. In addition, pandan leaves have specific flavor and contain carotenoid, phenolic, and flavonoid substances. Anatomically, the mesophyll’s size was greatest in the mature leaves, while the size of chloroplast was not significantly different from medium to over mature leaves. Based on the chlorophyll content and mesophyll size, it was concluded that mature pandan leaves were the best source of chlorophyll, containing chlorophyll of 623.08 mg/100 g dry weight (DW).

Surfactants are used to reduce surface and interfacial tension to form emulsions. Polysaccharides such as Porang Glucomannan (PG) with high viscosity can be used as surfactants. This research aimed to optimize the concentration of sodium carbonate (Na2CO3) and octenyl succinic anhydride (OSA) in modifying PG using a microwave. The optimization process is carried out using response surface methodology (RSM) with a two-factor central composite design (CCD), namely concentration of Na2CO3 (0.17–5.834%) and OSA (2.17–7.83%). The result showed that the concentration of Na2CO3 and OSA strongly influences emulsion capacity and stability. The optimum conditions that resulted in the highest emulsion capacity and stability were obtained at concentrations of Na2CO3 and OSA which were 2.25% and 6.19%, respectively. Degree of Substitution (DS), FTIR analysis, contact angle, and increased viscosity confirmed that OSA substitution occurred in PG. The characteristics of OSA-modified porang glucomannan (PGOS) such as: emulsion capacity and stability, Degree of Substitution (DS), contact angle, and viscosity increased to 34.6% and 32.5%, 1.02%, 92o, 5720 cP, respectively. FT-IR analysis confirmed the presence of OSA substitution at 1734 cm−1. PGOS can be used as a surfactant or gelator in oleogel production.

Klutuk banana leaf is widely used as food packaging material since it has large size and not easily torn. Many traditional packaging materials are recently abandoned, thus it is necessary to develop an active packaging having an advantage of synthetic packaging. This study aimed to create active packaging from methylcellulose (MC) added with glutaraldehyde (GA) and Klutuk banana leaf extract (EDPK), as well as to determine its properties and antioxidants. The casting method with GA as a crosslinker and EDPK as an antioxidant agent was used to make film/active packaging. Banana Klutuk leaves were dried using cabinet dryer for 24 hours and the leaf powder was extracted using maceration method with methanol 80%. The extract was concentrated with a rotary evaporator to be tested for its antioxidant capacity. The results showed that the film with EDPK addition exhibited dark green color. Tensile strength values increased when EDPK and GA were added at concentrations of 30 and 45%. However, EDPK film elongation was not affected by GA addition. Addition of EDPK decreased thermal stability by 25-45 oC, whereas the addition of GA improved thermal stability by 5-25 oC. Antioxidants in banana leaf extract can migrate to 10 and 50% simulant ethanol.

Abstract Asia represents rich traditional dietary diversity. The rapid diet transition in the region is leading to a high prevalence of non-communicable diseases. The aim of this exploratory study was to document traditional foods and beverages and associated traditional knowledge that have potential positive health impacts, from selected countries in the region. The study also focused on identifying their importance in the prevention and management of lifestyle-related diseases and nutritional deficiencies as well as for the improvement of the overall health and wellbeing. This was conducted in selected locations in Indonesia, Thailand, Malaysia and Nepal through a qualitative method with a pre-tested documentation format. Through a detailed documentation of their health benefits, the study tries to highlight the significance of traditional foods in public health as well as their relevance to local market economies towards sustainable production and consumption and sustainable community livelihoods.

Gelatin merupakan protein yang diperoleh dari hidrolisis parsial kolagen yang ditemukan di dalam kulit, tulang, dan jaringan ikat hewan. Pada umumnya, gelatin diekstrak dari kulit sapi dan babi yang mempunyai permsalahan terkait isu penyakit sapi gila dan kehalalanya. Tujuan penelitian ini adalah untuk mengetahui karakteristik gelatin yang diesktrak dari kulit kerbau dan aplikasinya sebagai enkapsulan pada mikroenkapsulasi minyak atsiri daun cengkeh. Gelatin kulit kerbau diekstrak menggunakan larutan asam klorida (Tipe A) 1%, 70 °C selama 5 jam. Gelatin kulit kerbau diaplikasikan sebagai enkapsulan pada mikroenkapsulasi (spray drying) minyak atsiri daun cengkeh menggunakan rancangan acak lengkap (RAL) dengan faktor perbedaan konsentrasi gelatin (5%, 7.5%, dan 10%) dan rasio antara minyak atsiri daun cengkeh dengan gelatin (1:10, 1:15 dan 1:20). Karakteristik gelatin kulit kerbau mempunyai kadar air 7.4%, kadar abu 0.06%, kadar lemak 0.04%, kadar protein 89.1%, viskositas 4.8 cP dan kekuatan gel gelatin 155.39 g Bloom. Karakteristik mikrokapsul minyak atsiri daun cengkeh mempunyai kelarutan 79.79%, total minyak 1.10 mg/ml, minyak terperangkap 0.87 mg/ml, dan minyak dipermukaan 0.23 mg/ml. Morfologi mikrokapsul minyak atsiri daun cengkeh berbentuk oval dan tidak beraturan.

Coconut (Cocos nucifera) fruit has attracted consumer interest due to its health benefits, especially the consumption of coconut water produced from 6–7-month-old coconut fruit. Consequently, by-products from young coconut fruit are also being expanded, including coconut mesocarp containing phenolic compounds. Therefore, this study aimed to provide new applications for young coconut mesocarp wastes. Specifically, optimizing the ultrasound-assisted extraction (UAE) of phenolic compounds from coconut mesocarp using a Box–Behnken design in conjunction with response surface methodology (RSM). The effects of three extraction variables, such as temperature (10–70 °C), solvent-to-sample ratio (20:1–10:1 mL g−1), and pulse duty cycle (0.4–1.0 s−1) were examined on the level of total phenolic content (TPC) and antioxidant activity of the extract. Subsequently, the optimum UAE condition was predicted using RSM models with coefficients of determination (R2) higher than 0.94, low prediction errors (less than 2.34), and non-significant lack-of-fit values (p < 0.05) for the two responses. Extraction time was evaluated through kinetic (5 to 25 min) studies applying the optimum extraction temperature (70 °C), solvent-to-sample ratio (20:1 mL g−1), and pulse duty cycle (0.55 s−1). An efficient extraction was achieved within 5 min, resulting in an extract with 47.78 ± 1.24 mg GAE 100 g−1 DW for the total phenolic compounds and high antioxidant activity (87.28 ± 1.01% DPPH). Extraction by ultrasound was then concluded to facilitate a fast extraction rate with high reproducibility (coefficients of variation were less than 3% in the levels of antioxidant activity and phenolic compounds).

Photooxidation is one of the causes of quality deterioration in food. An antioxidant or singlet oxygen quencher is urgently needed to prevent photooxidation. γ-Oryzanol was recognized as a naturally present antioxidant in rice bran products. This research aimed to calculate the singlet oxygen quenching rate and its mechanism of γ-oryzanol to evaluate the potency of γ-oryzanol as singlet oxygen quencher. A series of linoleic acid (50 and 100 mM) or γ-oryzanol (0.7 and 1.5 mM) were prepared separately in ethanol: chloroform (96:4, v/v) containing 25 ppm of erythrosine. High-Performance Liquid Chromatography quantified the degradation of γ-oryzanol. Meanwhile, Gas Chromatography determined the changes in linoleic acid content during photooxidation. The singlet oxygen quenching rate was calculated by steady-state. The singlet oxygen quenching rate of γ-oryzanol was 3.04 × 106/M/s by physical and chemical quenching mechanism. Photooxidation caused the declined of γ-oryzanol by 0.1421 mM/h. Based on singlet oxygen quenching rate calculation, it suggests that γ-oryzanol can perform as a singlet oxygen quencher with slightly dominated by physical quenching mechanism (52.28%). The rest it performed via a chemical quenching mechanism.

Kabau, an unexplored crop, was analyzed to determine its nutrition and antinutrition components and in vitro protein digestibility (IVPD). Some conventional processes, such as steaming, frying, and boiling, were carried out to study their effect. The results indicated that all of the techniques reduced carbohydrate content. Frying significantly increased the fat content of Kabau and reduced other nutritional components. In general, all the methods significantly reduced phytic acid, tannin, and trypsin inhibitors, as much as 94.95–96.26%, 20–35%, and 89.22–92.88%, respectively. The reduction of antinutritional components resulted in higher IVPD on boiled and steamed Kabau, 69.47% and 61.48%, respectively.

Coconut sap is sweet, oyster-white and translucent liquid which can be obtained by tapping coconut flowers for 8 ̶ 12 hours. Since it contains sugar with nearly neutral pH, coconut sap is highly susceptible to spontaneous fermentation. In order to maintain the quality of the sap during the tapping process, coconut farmers usually add preservative substances. The preservatives can be produced either naturally, such as mixtures of lime, mangosteen peel and sliced jackfruit wood, or synthetic one, such as sodium metabisulphite. The chemical properties of coconut sap might be affected by weather conditions during the tapping process. This research aimed to determine the effect of weather conditions during tapping process and variations of mangosteen peel powder concentration on the chemical properties and antioxidant activity of the coconut sap. The results showed that the optimum preservative concentration of mangosteen peel powder was 0.84 g/L of coconut sap which was tapped in sunny weather condition. This treatment yielded sap with chemical properties i.e. pH value of 6.7; total soluble solids of 16.2 °brix; water content of 85.62%; reducing sugar of 0.04 g/100 g; sucrose content of 13.64 g/100 g; total free amino acids of 0.1738 g/100 g; total phenolic of 323.23 mgGAE/100 g; and vitamin C of 26.06 mg/100 g. The antioxidant activity of this treatment was 34.39 % RSA evaluated by DPPH method, this value, was relatively similar to that of the addition of 0.28 and 0.56 g/L mangosteen peel powder. The coconut sugar produced had reducing sugar of 5.01 g/100 g; total sugar of 86.50 g/100 g; total phenolic content of 470 mgGAE/100 g; browning index of 16254 IU (ICUMSA); and antioxidant activity of 61.05% RSA.

Minuman jamu kunyit asam adalah minuman herbal khas Indonesia yang terbuat dari rimpang kunyit, buah asam jawa, gula kelapa, air dengan atau tanpa penambahan sari jeruk nipis dan ekstrak daun sirih. Proses produksi dilakukan secara tradisional dan banyak dilakukan oleh industri rumah tangga. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan kualitas mikrobiologis dari variasi waktu perebusan dan formula minuman jamu kunyit asam. Waktu perebusan terdiri atas 2,5 dan 7,5 menit serta variasi formula yaitu minuman jamu kunyit asam (KA), KA dengan penambahan sari jeruk nipis, dan KA dengan penambahan ekstrak daun sirih. Rancangan Penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan tiga ulangan. Variabel yang diamati meliputi pH, total padatan terlarut, total fenolik, total flavonoid, total curcumin kasar, aktivitas antioksidan (metode DPPH‘Radical Scavenging Activity dan Ferric Reducing Antioxidant Power), dan total mikrobia. Hasil penelitian menunjukkan bahwa perebusan 7,5 menit menyebabkan kualitas mikrobiologis dan total flavonoid minuman lebih tinggi dan total curcumin lebih rendah dibandingkan perebusan 2,5 menit. Waktu perebusan tidak berpengaruh terhadap aktivitas antioksidan, total fenolik dan pH. Adanya penambahan sari jeruk nipis pada formula minuman jamu kunyit asam menyebabkan kenaikan total flavonoid. Penambahan ekstrak daun sirih menyebakan total fenolik, aktivitas antioksidan, dan kualitas mikrobiologis minuman jamu kunyit asam sirih lebih tinggi dibandingkan formula minuman lainnya. Secara umum, perlakuan panas dan penambahan ekstrak daun sirih dapat meningkatkan aktivitas antioksidan dan kualitas mikrobiologis minuman jamu kunyit asam.

This research focused on studying the effects of orally administered pressure-blanched white saffron on the antioxidative properties and lipid profiles of wistar rats. White saffron was blanched in autoclave for 2.5, 5, 7.5, and 10 min at 100, 105, 110, 115, and 120 °C, which are equivalent to 14.71, 17.53, 20.79, 24.54, and 28.81 psia, respectively. A total of 30 male wistar rats aged four weeks were fed with a standard diet (N), oxidized peanut oil diet + unblanched white saffron (A), oxidized peanut oil diet + blanched white saffron (B), oxidized peanut oil diet + pressure-blanched white saffron (C), and oxidized peanut oil diet + aquadest (NC), for two weeks after pre-treatment with the standard diet for a week. In vivo study showed treatment with pressure-blanched white saffron could significantly improve SOD, Vitamin E, and HDL levels compared to the negative control (NC); 686.44 U/g Hb, 10.87 μg/mL, and 94.17 mg/dL versus 405.37 U/g Hb, 7.44 μg/mL, and 43.47 mg/dL, respectively. Meanwhile, treatment with pressure-blanched white saffron could significantly reduce MDA, total cholesterol, LDL, and triglyceride levels in the blood compared to the negative control (NC); 1.98 mmol/L, 108.74 mg/dL, 40.99 mg/dL, and 78.06 mg/dL versus 8.54 mmol/L, 232.46 mg/dL, 149.17 mg/dL, and 172.61 mg/dL, respectively. The results showed that pressurized blanching could significantly increase antioxidant levels of white saffron, and its dried form could improve antioxidative properties and lipid profiles in vivo.

Jengkol (Pithecellobium jiringa) is a typical plant in the tropical region of Southeast Asia that is mostly liked by indonesian people because of savory taste. Jengkol seeds having high protein and healthy effects need processing for keeping their nutrient quality. This study aims to determine the effect of steam blanching on the non-volatile taste components of jengkol seeds. The results showed that steam blanching during 5 min decreased the total content of soluble sugars from 998.10 to 903.59 ppm, organic acid from 442.80 to 362.89 ppm and 5ʹ-nucleotides from 2.92 to 2.57 mg/g dry weight. However, steam blanching could be able to increase the total content of free amino acids from 40.99 to 44.98 mg/g dry weight. The EUC (equivalent umami concentration) value in jengkol seeds also increased from 38.27 to 42.22 g MSG/100 g dry weight. The total content of amino acids also increased slightly from 9.81% to 10.08% (w/w) on jengkol seed flour after blanching treatment. Jengkol seed flour consists of hydrophobic amino acids (36.00–34.41%). The E/T value decreased from 35.54% to 33.92% but the PER (protein efficiency ratio) value increased from 2.43 to 2.63 on jengkol seed flour after steam blanching treatment. The steam blanching treatment increased the MSG-like components in jengkol seed flour and also gave the sweet taste and prevented the formation of the bitter taste. Hence, jengkol seed flour showed a high potential to serve as a functional food with a umami taste.

Coconut is widely used as a food source in producing countries, and during consumption, the waste that is generated needs to be reduced through by-products processing to ensure environmental sustainability. This study aimed to assess the functionality of by-products (endo- and mesocarp) of coconuts at early and mature stages. The aqueous and ethanolic (50 and 100% ethanol in water) extracts of coconut by-products were evaluated for the DPPH radical scavenging activity and subjected to linoleic acid-β-carotene system assay in contrast with synthetic antioxidants. Ultrasound-producing extract of young coconut mesocarp provided the highest antioxidant activity with a lower IC50 value (117 µg mL−1) than butylhydroxytoluene (BHT, 170 µg mL−1). Based on the linoleic acid-β-carotene system assay, the extract exhibited a higher antioxidant activity (1.25×) than tertiary butylhydroquinone (TBHQ, 200 µg mL−1); and comparable with butylhydroxyanisole (BHA, 250 µg mL−1). Therefore, extracts of coconut by-products, particularly the young mesocarp, can be an alternative natural antioxidant.

Kerandang (Canavalia virosa) can be found in sandy beaches in Kulon Progo and Bantul Regency, Yogyakarta. The kerandang seeds can be processed into kerandang milk such as soybean, as one form of product diversification. The objective of this study was to know the physicochemical characteristics of the kerandang milk powder. Peeled kerandang seeds were processed into kerandang milk with seed water ratio of 1:8 (w/v). The addition of maltodekstrin (0%, 5%, 7.5% and 10% (w/v)) into kerandang milk, then dried using a spray dryer with inlet temperature of 80 °C; 100 °C, and 120 °C. Analyses were conducted on the water content, bulk density, solubility, water holding capacity (WHC), fat holding capasity (FHC), soluble protein, total phenolic, genistein, and antioxidant activity. This study was conducted using acompletely randomized design with three replications. The results of this study showed that Water Holding Capacity of kerandang milk ranged of 2,98% to 64,55%. Inlet temperature, maltodextrin concentration and interaction of these two factors gave significant effects on the WHC of kerandang milk powder. FHC of kerandang milk powder increased (108.89%–262.25%) with the higher concentration of maltodextrin and spray inlet temperature. The bulk density of kerandang milk powder was between 0.34g/mL–0.58g/mL. Kerandang milk powder solubility at various pH was closely related tot he protein content in milk powder kerandang. The dissolved protein content of kerandang milk powder decreased with the increased of maltodextrin concentration and inlet temperature. The content of total phenolic of kerandang milk powder ranged from 1.33 gGAE/100g to7.55 gGAE/100g. The antioxidant activity of the kerandang milk ranged between 9.61% to 74.87%. ABSTRAK Kerandang (Canavalia virosa) dapat dijumpai di sepanjang lahan pasir pantai di Kabupaten Kulonprogo dan Bantul, Yogyakarta. Biji kerandang dapat diolah menjadi sari kerandang seperti halnya kedelai, sebagai bentuk diversifikasi produk. Penelitian ini bertujuan untuk mengetahui karakteristik fisik dan kimia bubuk sari kerandang. Biji kerandang kupas (tanpa kulit ari) diolah menjadi sari kerandang dengan perbandingan biji dan air 1:8 (b/v). Penambahan maltodekstrin sebanyak 0%; 5%; 7,5%; dan 10% (b/v) padasari kerandang, kemudian dikeringkan menggunakan spray dryer dengan suhu inlet 80 °C; 100 °C; dan 120 °C. Analisis yang dilakukan meliputi kadar air, bulk density, kelarutan, water holding capacity (WHC), fat holding capasity (FHC), protein terlarut, total fenolik, genistein, dan aktivitas antioksidan. Percobaan dilakukan menggunakan rancangan acak lengkap dengan ulangan percobaan sebanyak tiga kali. Hasil penelitian menunjukkan bahwa kemampuan menahan air (WHC) bubuk sari kerandang berkisar antara 2,98%–64,55%. Konsentrasi suhu inlet dan maltodekstrin serta interaksi keduanya memberikan perbedaan nyata terhadap WHC bubuk sari kerandang. FHC bubuk sari kerandang (108,89%–262,25%) mengalami peningkatan dengan bertambahnya konsentrasi maltodekstrin dan temperatur inlet spray dryer. Bulk density bubuk sari kerandang berkisar antara 0,34 g/mL–0,58 g/mL. Kelarutan bubuk sari kerandang di berbagai pH berhubungan erat dengan kandungan protein dalam bubuk sari kerandang. Protein terlarut bubuk sari kerandang menurun dengan semakin meningkatnya konsentrasi maltodekstrin dan temperatur inlet. Total fenolik bubuk sari kerandang berkisar antara 1,33 g GAE/100 g–7,55 g GAE/100 g. Aktivitas antioksidan bubuk sari kerandang berkisar antara 9,61%–74,87%. Kata kunci: Karakteristik; bubuk sari kerandang; fisiko-kimia

Penggunaan katalis asam homogen memiliki kelemahan dalam pemisahan katalis dari media reaksi. Oleh karena itu, diperlukan pengembangan katalis heterogen (padat) yang lebih mudah dipisahkan dari media reaksi sehingga dapat menyederhanakan tahap produksi. Penelitian ini bertujuan untuk memodifikasi tandan kosong kelapa sawit (TKKS) menjadi katalis asam heterogen (padat) melalui proses oksidasi dan sulfonasi. Pada proses oksidasi, lignoselulosa diubah menjadi aldehid, kemudian dilanjutkan dengan sulfonasi untuk mengubah gugus karbonil menjadi sulfonat. TKKS di-pretreatment terlebih dahulu, kemudian dioksidasi menggunakan sodium periodat (rasio 0,8) pada suhu dan waktu bervariasi (40, 50 dan 60 oC, selama 3, 6, 9 dan 12 jam) dalam waterbath shaker 150 strokes/min dengan kondisi tanpa cahaya. Selanjutnya dilakukan reaksi oksidasi sebanyak dua tahap diikuti dengan sulfonasi. TKKS tersulfonasi kemudian diaplikasikan untuk mengkatalisis reaksi esterifikasi etil oleat. Suhu dan waktu reaksi oksidasi berpengaruh sangat signifikan (p kurang dari 0,01) terhadap kandungan gugus karbonil yang terbentuk. Perlakuan pada suhu 50 oC selama 9 jam mampu menghasilkan gugus karbonil 98,34 ±1,52 μmol/g-sampel. Reaksi oksidasi dua tahap mampu meningkatkan gugus karbonil 12,02 % lebih besar. Reaksi sulfonasi pada suhu 45 oC selama 3 jam hanya mampu mengkonversi 27,97 % gugus karbonil menjadi sulfonat dengan kandungan total sulfur 6,30 ±0,29 %, hal ini berdampak kepada kemampuan TKKS tersulfonasi untuk mengkatalisis reaksi esterifikasi etil oleat dengan yield yang masih rendah yaitu 29,12 ±5,15 %.

The study was investigated the functional properties of buffalo skin gelatine. Gelatine was extracted from swamp buffalo skin using crude acid protease from cow’s abomasum (CAPC) in concentration variation 0; 2.5; 5; and 7.5 U/mg. The temperature to hydrolysis included at 28 °C, 37°C and 40°C. The emulsion activity index (EAI), Emulsion stability index (ESI), foaming expansion (FE) and foaming stability (FS) were investigated. The interaction between CAPC concentration and hydrolysis temperature has a significant effect (P

In recent years, there is increasing demand of gelatin application, particularly in confectionary products such as marshmallow. Due to halal issue of predominant porcine gelatin, alternative gelatin from other source, like buffalo hide, is critically needed. This study aimed to analyze physical and chemical properties of marshmallow made from buffalo hide gelatin, which was compared to commercial marshmallow and those made using commercial gelatin. Conducted in two stages, the first part was gelatin extraction from buffalo hide and its quality analyses. The second was marshmallow preparation from the gelatin and its analyses. Concentration of buffalo hide gelatin was 4%, 5%, dan 6%, whereas commercial gelatin was 7%, 8%, and 9%. The experiment was repeated three times and all determination were performed in duplicated. Results showed that marshmallow from buffalo hide gelatin contained 15,5 – 18,40 % (wb) moisture; 0,64 - 1.16% (db) ash content; 43,34 – 48,83 % (db) total sugar; and 24,25 – 28,73 % (db) reducing sugar. Textural analyses indicated that the highest value of marshmallow hardness (129.50 g/mm2) and highest value of springiness (5.70 mJ/mm2) were obtained by marshmallow made using 5% and 6% buffalo hide gelatin, respectively), thus concluded that buffalo hide is potential halal gelatin source to produce marshmallow

All parts of Syzygium cumini Linn. (duwet) were widely used for medicinal plant in the treatment of various deseases. The seed extract was used to lower blood glucose. Cumini's seed had higher phenolic fractions than others. It were prepared by extracted of duwet seed "Genthong" varieties, using various extractants such as 85% ethyl acetate, 50% methanol and 50% ethanol. Duwet seed extract collected then was determined of phenolics compound and antioxidant activity assayed by measuring 2,2-diphenyl-1-picrylhydrazylradical (DPPH) scavenging activity, reducing Ferric ion (Fe3+) power and inhibition of linoleic acid oxidation. The objective was to select one of the extractant which gave highest yield and polyphenolic content and its stronger antioxidant activity. Extractant 50% methanol gave highest the extract yields was 16.29 % (db.) and phenolics compounds of extract was composed: total phenolic 45.99±0.25 g-GAE/100 g-extract; total flavonoid 2.28±0.07 g-QE/100 g-extract and total tannin 26.9±0.07 g-TAE/100 g-extract. All of extract exhibited strong on behalf of RSA-DPPH assay 87-95% (100 g-mL-l) and reducing power, nevertheless in inhibition of linoleic acid oxidation was moderate 49-55% (400 g-mL-l). Keywords: Syzygium cumini Linn, seed, antioxidant, extraction ABSTRAK Tanaman duwet (Syzygium cumini Linn.) pada semua kompartemennya dimanfaatkan masyarakat untuk pengobatan suatu penyakit. Ekstrak bijinya dimanfaatkan untuk penurun gula darah. Bijinya merupakan bagian tanaman yang kaya senyawa polifenol. Fraksi kaya senyawa fenolik dipreparasi dengan mengekstraksi biji duwet varietas "Genthong", dengan tiga jenis ekstraktan; etil acetat 85%, metanol 50% dan etanol 50%. Ekstrak biji duwet (EBD) yang diperoleh dianalisis kelompok senyawa fenolik dan aktivitas antioksidan menggunakan metode uji penangkapan radikal DPPH (2,2-diphenil 1-picrylhydrazyl), uji reduksi ion Feri (ferric reduction antioxidant power-FRAP) dan uji penghambatan peroksidasi asam lemak linoleat. Tujuan penelitian adalah memilih satu dari tiga ekstraktan yang menghasilkaan ekstrak, dengan yield dan kadar senyawa polifenolik terbesar serta sifat antioksidatif terkuat. Yield dengan ekstraktan Met-OH-50%, sebesar 16,29% (db), senyawa fenoliknya sebesar 45,99 ±0,25 g-GAE/100 g-EBD; 2,28±0,07 g-QE/100 g-EBD dan 26,9±0,07 g-TAE/100 g-EBD. Ketiga ekstrak kuat dalam uji penangkapan radikal DPPH antara 87-95 % (100 g-mL-l) dan uji reduksi ion Feri (Fe3+), moderat pada uji penghambatan peroksidasi lipid, 49-52 % pada 400 g- mL-l. Kata kunci: Biji duwet (Syzygium cumini Linn.), antioksidan, ekstraksi

The experiment was conducted to determine the effect of CPO protected with formaldehyde on digestibility and performance of thin tail sheeps. Fifteen local male lambs aged 9-12 months, were divided into 3 groups of ration treatment. The first group received only the basal ration (R0), the second group received the basal ration and 3% CPO (R1), while the third group received the basal ration and 3% CPO protected with 2% formaldehyde (R2). The data were analyzed by Completely Randomized Design with oneway pattern. The different of treatments were tested by Duncan’s New Multiple Range Test. The results showed that addition CPO protected with formaldehid (R2) in the sheeps diet did not affect dry matter and organic matter intake, dry matter and organic matter digestibility, average daily gain (ADG), but significantly (P

The study was aimed to investigate chemical composition, dietary fiber content and the antioxidant activity of the hydrophilic extract of varieties of rice bran IR-64, Sintanur, Rajalele and Menthikwangi. Rice bran from each sample was analyzed proximate and fiber content. Hydrophilic extract was obtained by extracting each sample of bran with methanol solvent. Total phenolic content was tested by Folin Ciocalteu reagent and antioxidant activity of the extract was tested by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results showed that all the four samples had differences amount of their moisture, ash, protein and fat contents. Fat content varies from 16.80±0.05 % - 23.75±0.04 %db, which the highest level of fat content was possessed by Sintanur bran (23.75±0.04 %db) and IR-64 bran (16.80±0.05 %db) was the lowest level. The levels of soluble fiber from four varieties of rice bran were not significantly different, between 4.07 – 4.14 %db but there was a difference in the content of insoluble fiber, which had effect on the total of fiber content. The insoluble fiber content of the rice bran varieties of Menthikwangi, Rajalele, IR-64 and Sintanur were 27.64±0.46, 28.04±0,25, 29.15±0.26 dan 30.44±0.60 %db respectively. The content of total phenols and the highest antioxidant activity of the hydrophilic extract was owned by Menthikwangi ie. 2794.28±181.83 μg galic acid equivalen (GAE)/g bran for total phenol and 41,28±0,60 % for antioxidant activity. Keywords: Rice bran, hydrophilic extract, rice variety, phenol, DPPH ABSTRAK Penelitian bertujuan untuk mengetahui komposisi kimia, kadar serat pangan serta aktivitas antioksidan ekstrak hidrofilik bekatul dari empat varietas padi yaitu IR-64, Sintanur, Rajalele dan Menthikwangi. Masing-masing sampel bekatul dianalisis proksimat dan kadar serat pangannya. Ekstrak hidrofilik diperoleh dengan cara mengekstrak masingmasing sampel bekatul dengan pelarut metanol. Ekstrak diuji kandungan total fenol dengan reagen Folin Ciocalteu dan aktivitas antioksidannya dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan bahwa keempat sampel memiliki kadar air, kadar abu, kadar protein dan kadar lemak yang bervariasi. Kadar lemak bervariasi dari 16,80±0,05 % - 23,75±0,04%db, dengan kadar tertinggi dimiliki bekatul varietas Sintanur (23,75±0,04 %db) dan terendah bekatul varietas IR-64 (16,80±0,05 %db). Kadar serat larut (soluble fiber, SF) bekatul dari keempat varietas padi tidak berbeda secara signifikan yaitu antara 4,07 - 4,14 %db namun terdapat perbedaan pada kandungan serat tak larut (insoluble fiber, IF) yang berpengaruh pada kadar serat total. Kandungan serat tak larut bekatul dari varietas Menthikwangi, Rajalele, IR-64 dan Sintanur berturut-turut adalah 27,64±0,46, 28,04±0,25, 29,15±0,26 dan 30,44±0,60 %db. Kandungan total fenol dan aktivitas antioksidan tertinggi dimiliki oleh ekstrak hidrofilik dari bekatul verietas Menthikwangi, masing-masing 2794,28±181,83 μg ekivalen asam galat (EAG)/g bekatul untuk total fenol dan aktivitas antioksidan menangkal (scavenging) radikal bebas (DPPH) sebesar 41,28±0,60 %. Kata kunci: Bekatul, ekstrak hidrofilik, varietas padi, fenol, DPPH

A s tudy on the distribution of plumbum, cadmium on soybeans and deprotonation of carboxyl functional groups of citric acid in the chelation process. This study was deprotonation phase of carboxyl functional groups of citric acid as chelating agent, that was review chemically activity (protons number) functional group of citric acid was caused of chelation environment pH changes. Research factors were 1) ratios of citric acid with water, consists of three levels i.e. 0,1:1; 0,2:1 and 0,3:1 second factors was chelation environment pH, consists of three levels i.e. 5; 7.5 and 10. The results showed that, the plumbum (Pb) accumulation in seed coat tissue average value 0,37 ± 0,03 ppm, whereas Pb on cotyledon tissue average value 0,39 ± 0,07 ppm. Cadmium (Cd) accumulation in seed coat tissue average value was 0,02 ± 0,004 ppm while the Cd on cotyledon tissue average value 0,02 ± 0,003 ppm. The highest deprotonation of functional groups citric acid as chelating agent 9,43cmol+kg was reached on chelation condition citric acid ratios with water 0,3:1 on chelation environment pH 10 (A3B3–1). The study result non significant difference with citric acid ratios with water 0,2:1 and 0,1:1 on chelation environment pH 10. Keywords: Soybean, plumbum, cadmium and deprotonation ABSTRAK Telah dilakukan penelitian tentang distribusi plumbum, cadmium pada biji kedelai dan deprotonasi gugus fungsional karboksil asam sitrat dalam proses khelasi. Penelitian ini merupakan tahapan deprotonasi gugus fungsional karboksil asam sitrat, yaitu tinjauan secara kimiawi keaktifan (jumlah proton) gugus fungsional asam sitrat akibat perubahan pH lingkungan khelasi. Faktor penelitian meliputi ratio asam sitrat terhadap air yang terdiri dari tiga taraf 0,1:1, 0,2:1 dan 0,3:1 dengan pH lingkungan khelasi yang terdiri dari tiga taraf 5; 7,5 dan 10. Hasil penelitian menunjukkan bahwa, akumulasi plumbum (Pb) pada jaringan kulit biji kedelai rerata 0,37 ± 0,03 ppm, sedangkan Pb pada jaringan kotiledon rerata 0,39 ± 0,07 ppm. Akumulasi cadmium (Cd) pada jaringan kulit biji kedelai rerata 0,02 ± 0,004 ppm sedangkan Cd pada jaringan kotiledon rerata 0,02 ± 0,004 ppm. Deprotonasi gugus fungsional chelating agent asam sitrat tertinggi 9,43 cmol + kg –1 dicapai pada kondisi khelasi dengan ratio asam sitrat terhadap air 0,3:1 pada lingkungan khelasi pH 10 (A). Keadaan tersebut tidak berbeda nyata dengan perlakuan ratio asam sitrat 0,2:1 dan 0,1:1 pada lingkungan khelasi pH 10. Kata kunci: Kedelai, plumbum, cadmium dan deprotonasi

A study on the chelation of plumbum and cadmium by citric acid in soybean seeds, it was aimed to identify the effect of chelation treatment (chelating ratio, time contact and seeds condition) on plumbum reduction and cadmium in soybean seeds. A factors of research covering 1st) chelating ratio (citric acid/”C”) consisting of three (1, 2 and 3 grams of citric acid); 2nd) the time contact (“D”) consisting of three (90, 120 and 180 minutes) and the 3rd factors is condition of the seeds (“E”) consisting of three standard (whole qualify e.i. passing sieve of 0.3 inch and restrained at 0.2 inch), ruptured e.i. restrained for sieve at 10 mesh, and flour qualify for pass for sieves 50 mesh. The result showed that the combination of three factors were able to reduce Cd maximaly (non detected/detect limit 0.01 ppm). Reduction Pb maximaly (non detected /detect limit 0.09 ppm) occured in treatment C1D1E1, C1D1E2, C1D2E1, C1D2E2, C1D2E2, C2D1E1, C2D1E2, C2D2E1, C2D3E1, C2D3E2, C3D1E1 and C3D2E1. A combination of citric acid chelation (C) with the time contact (D) showing significant difference and both showed interactions, the reduction of the highest was C1D1 treatment. A combination of citric acid chelation (C) with the condition of the nut (E) showed significant difference and both no interaction, the reduction of the highest was C2E1 treatment. The combination between the time of contact (D) with the condition of the seed (E) showed significant difference and both no interaction, the reduction of the highest were D1E1 and D2E1 treatments. Keywords: chelation, plumbum, cadmium and soybean ABSTRAK Telah dilakukan penelitian tentang khelasi plumbum (pb) dan cadmium (cd) menggunakan asam sitrat pada biji kedelai, dengan tujuan untuk mengetahui pengaruh faktor perlakuan khelasi (ratio pengkhelat, waktu kontak dan kondisi biji) dalam mereduksi Pb dan Cd pada biji kedelai. Faktor penelitian meliputi 1) rasio pengkhelat (asam sitrat/C) yang terdiri dari tiga taraf (1, 2 dan 3 gram asam sitrat); 2) waktu kontak (D) yang terdiri dari tiga taraf (90, 120 dan 180 menit) serta faktor ke-3) kondisi biji (E) yang terdiri dari tiga taraf (utuh yaitu lolos pada saringan 0.3 inch dan tertahan pada 0.2 inch, pecah yaitu tertahan pada saringan 10 mesh dan tepung yaitu lolos pada saringan 50 mesh. Hasil penelitian menunjukkan bahwa kombinasi ketiga faktor, mampu mereduksi Cd secara maksimal (tidak terdeteksi/batas deteksi 0,01 ppm). Reduksi Pb tertinggi adalah maksimal (tidak terdeteksi/batas deteksi 0,09 ppm) terjadi pada perlakuan C1D1E1, C1D1E2, C1D2E1 dan C1D2E2; C2D1E1, C2D1E2, C2D2E1, C2D3E1 dan C2D3E2; C3D1E1 dan C3D2E1. Kombinasi antara pengkhelat ”asam sitrat” (C) dengan waktu kontak (D) menunjukkan berbeda nyata dan keduanya menunjukkan interaksi, reduksi tertinggi dicapai perlakuan C1D1. Kombinasi antara pengkhelat ”asam sitrat” (C) dengan kondisi biji (E) menunjukkan berbeda nyata dan keduanya tidak ada interaksi, reduksi tertinggi dicapai perlakuan C2E1. Kombinasi antara waktu kontak (D) dengan kondisi biji (E) menunjukkan berbeda nyata dan tidak ada interaksi, reduksi tertinggi dicapai perlakuan D1E1 dan D2E1. Kata kunci: khelasi, plumbum, cadmium dan kedelai

This research aimed to determine the effect of crude palm oil protected with formaldehyde on the hydrogenation of unsaturated fatty acids in the rumen and its effect on blood and meat fatty acids. Fifteenth local male lambs aged 9-12 months weighing 14-17 kg, were divided into 3 groups ration treatment. The first group received only the basal ration (R0), the 2nd group received the basal ration and 3% CPO (R1), while the 3rd group received the basal ration and 3% CPO protected with 2% formaldehyde (R2). Basal feed consisted of 60% grass, 30% bran and 10% soybean meal, with the nutrient content of 62.98% TDN, 45.5% DM, 14.48% CP, 4.70% EE and 21.93% CF. Parameters observed were the fatty acid from rumen fluid, blood and meat of sheep. Data were analyzed by complete randomized design direction patterns. Differences between treatments were tested further using Duncan's New Multiple Range Test. The results showed that treatment of R2 can increase unsaturated fatty acids in the rumen, blood and meat (P

This research aimed to know the ability of formaldehyde to protect unsaturated fatty acid of CPO on the hydrogenation process by rumen microbes. In this experiment, the in vitro fermentation of rumen fluid was carrying out. It was taken from the rumen-trocar of female sheep. The unsaturated fatty acid source was from CPO (Crude Palm Oil) which encapsulated by formaldehyde 37% within 0%, 1%, 2% and 3%. The data was analyzed by Completely Random Design with Duncan's New Multiple Range Test. The difference of means the treatments were tested by Duncan's New Multiple Range Test. Result showed that oleic and linoleic resulting from fermenting CPO protected by formaldehyde was increase if it was compared with the unprotected CPO. It can be concluded that encapsulated CPO with formaldehyde was able to prevent hydrogenating of unsaturated fatty acid, mainly oleic and linoleic.

Carrageenan extracted from Eucheuma sp. red algae has potential benefit to develop edible film. Eucheuma Sp. red algae used in this research were harvested from Pantai Selatan, a beatiful beach lies at Gunung Kidul resort, Jogjakarta Province, Indonesia. First objective of this research was proximat analysis of coarse-ground Eucheuma sp. red algae. Thirdly, weight loss analysis of green grapes wrapped by carrageenan edible film. Coarse-ground Eucheuma Sp. algae containde water, ash, protein, fat, and carbohydrate (by different) respectively 11,20%, 18,19%, 15,42%, 2,19% and 53,0%. Carrageenan extracted from Eucheuma sp. contained lower sulphuric (13,79%) than commercial carrageenan (Wako Chem.). Carrageenan edible film wrapping was not effective to reduce weight loss of green grapes.

Penelitian ini bertujuan untuk mengidentifikasi penurunan aktivitas antioksidan dari ekstrak ubi jalar satsumaimo dan ubi merahyang diproses tanpa pemanasan dalam bentuk pati dan dengan pemanasan yang diproses menjadi keripik, digoreng dan dibuat tape. Penentuan total fenol dengan metode Folin Ciocalteu yang menggunakan standar asam galat dan evaluasi aktivitas antioksidan ekstrak ubi jalar dengan metode DPPH, yang ditunjukkan dengan penurunan absorbansi. Terdapat penurunan aktivitas antioksidan dan total fenol ekstrak satsumaimo atau ubi merah yang diproses dengan pemanasan dibanding tanpa pemanasan. Penurunan aktivitas antioksidan terbesar pada ubi jalar yang digoreng (44,7% ikatan radikal DPPH). Semakin lama dan semakin tinggi suhu proses pemanasan menyebabkan penurunan dari aktivitas antioksidan.

A study on the antioxidative properties of white saffron extract (in vivo) has been conducted. The purpose of this study was to determine the antioxidative effects of white saffron extract in in vivo assay. Fresh white saffrons were peeled, washed, blanched at 100˚C in 0.5% citric acid solution for 5 minutes and grated. The ratio between grated white saffron and distilled water was 1:1; 1:2; 1:3 and 1:4. Then it was filtered in order to obtain white saffron extract. The extract was evaluated in terms of its antioxidant activity by using in vivo. Five-week old male Wistar rats were purchased from Experimental Animal Development Unit, Gadjah Mada University. After one week of adaptation, the rats were divided into six groups, feed and drinking water were provided ad libitum. White saffron extract was orally administrated using a syringe at 09.00 a.m and 14.00 p.m daily, for 14 days. The livers and serum were removed for analysis of thiobarbituric acid reactive subtances (TBARS), α-tocopherols and superoxide dismutase (SOD). The results of this study showed that white saffron extract has an antioxidative activity in the in vivo assay. The higher concentration of white saffron extract, the higher α-tocopherols and superoxide dismutase, but the TBARS value was lower. Keywords: Curcuma mangga Val., rat, antioxidant activity, in vivo

Antioxidant Activity of Purifi ed Gambir Extract Using Sephadex LH-20 Column Chromatography ABSTRAK Telah dilakukan ekstraksi terhadap gambir menggunakan tiga jenis pelarut [aquades:etanol (1:1), etanol:etil asetat (1:1), dan etil asetat], dilanjutkan dengan purifi kasi menggunakan kromatografi kolom Sephadex LH-20. Kemudian diuji aktivitas antioksidannya dengan metode DPPH dan Ferritiosianat dalam sistem linoleat, yang dibandingkan dengan rutin dan BHT. Hasilnya menunjukkan bahwa ekstrak gambir terpurifi kasi (EGP) memberikan aktivitas penangkapan radikal DPPH dan penghambatan peroksida yang lebih tinggi dibanding rutin dan BHT. Aktivitas penangkapan radikal DPPH tertinggi dari EGP yang diinkubasi pada menit ke-25, pada konsentrasi 60 ppm, 80 ppm, dan 100 ppm, berturut turut yaitu fraksi etil asetat (27,58 % ± 1,87), fraksi etil asetat (tidak berbeda nyata dengan fraksi etanol:etil asetat) (34,05 % ± 0,96), dan fraksi etanol:etil asetat (42,54 % ± 0,73). Fraksi etanol:etil asetat menunjukkan penghambatan peroksida tertinggi (45,11 % ± 2,17 %), yang diinkubasi selama 10 hari. Kata kunci: Gambir, antioksidan, purifi kasi, DPPH, peroksida ABSTRACT Gambir was extracted with three types of solvents [aquadest:ethanol (1:1), ethanol;ethyl acetate (1:1), and ethyl acetate], followed by purifi cation using Sephadex LH-20 column chromatography. They were tested for antioxidant activity using DPPH and Ferrythiocyanate in linoleic system, compared with rutin and BHT. The results indicated that the purifi ed gambir extracts (PGE) gave the higher DPPH radical scavenging activity and inhibition of peroxide than rutin and BHT. The highest DPPH radical scavenging activity of PGE incubated for 25 minutes, at a concentration of 60 ppm, 80 ppm, and 100 ppm, were ethyl acetate fraction (27,58 % ± 1,87), ethyl acetate fraction (34,05 % ± 0,96), and etanol:ethyl acetat fraction (42,54 % ± 0,73), respectively. The fraction of ethanol:ethyl acetate displayed the highest inhibition of peroxide (45.11 % ± 2.17), incubated for 10 days. Keywords: Gambir, antioxidant, purifi cation, DPPH, peroxide

The Effect of Coconut Shell Liquid Smoke on the Growth of Fungi during Copra Drying and The Oil Quality Resulted ABSTRAK Metode pengawetan/pengasapan langsung secara tradisional terhadap kopra menyebabkan kopra yang dihasilkan berwarna coklat kehitaman karena endapan tar yang terlalu banyak, fl avor asapnya kuat dan prosesnya dapat menyebabkan polusi udara. Penelitian ini bertujuan untuk mengetahui perbedaan pengaruh pirolisat dan redistilat asap cair tempurung kelapa dalam menekan pertumbuhan jamur selama pengeringan, menentukan dosis dan lama perendaman daging buah kelapa, dan mengetahui pengaruh asap cair tempurung kelapa terhadap kualitas kopra dan minyak kelapa. Kualitas kelapa dipilih yang seragam, yaitu yang memiliki kualifi kasi grade A. Penelitian ini terdiri dari 3 faktor, yaitu: jenis asap cair (pirolisat dan redistilat), dosis asap cair (2 %, 6 %, dan 10 %), lama perendaman (5, 10, dan 15 menit). Perlakuan kontrol tanpa perendaman dengan asap cair sebagai pembanding. Kopra dengan tingkat serangan jamur terendah dan kontrol diekstrak minyak kelapanya kemudian dianalisis. Persentase serangan jamur terhadap kopra selama proses pengeringan dengan matahari langsung yaitu: perendaman dengan pirolisat serangan tertinggi sebesar 31,4 % (perlakuan 2 %, 5 menit), dan terendah 0 % (perlakuan 10 %, 15 menit; dan 10 %, 30 menit); sedangkan perendaman dengan redistilat serangan tertinggi 34,72 % (perlakuan 2 %, 30 menit), dan terendah 0 % (perlakuan 10 %, 15 menit; dan 10 %, 30 menit). Pada kontrol rata-rata cemaran serangan jamur sebesar 80,85 %. Hasil analisis minyak kelapa menunjukkan bahwa perlakuan perendaman dengan redistilat asap cair tempurung kelapa pada konsentrasi 10 % selama 30 menit memperlihatkan hasil yang paling baik dibandingkan dengan kontrol dan perlakuan lainnya dengan kadar air 2,45 %; kadar asam lemak bebas 0,26 %; angka peroksida 0,31 meg/kg ; bobot jenis 0,9130; indeks bias 1,452, dan skala warna 0,7. Kata kunci: Asap cair, tempurung kelapa, jamur, kopra, minyak kelapa ABSTRACT Direct preservation/smoking method has been done traditionally to inhibit the growth of fungi during drying and also storage of copra. By this method copra has black or brown black in color because of the deposit of tar and the smoke fl avor is too strong, and the process can caused air pollution. The aim of this research was to know the differences of pirolisate infl uence and redistilate liquid smoke of coconut shell in depressing growth of fungi during drying, dose determines and soaking time of fresh coconut and to know infl uence liquid smoke of coconut shell to copra quality and coconut oil. The quality of coconut was uniform and classifi ed as grade A quality. This research consisted of 3 factors, that was : type of liquid smoke (pirolisat and redistilat), smoke dose (2 %, 6 %, and 10 %), soaking time (5, 10, and 15 minutes). Then by contrast is applied control treatment without soaking with liquid smoke. Copra which have low level of fungi attack and control were extracted for oil that analyzed for its. The result of fungi attack indicated as follows; soaking with pirolisate highest attack 31,4 % (treatment 2 %, 5 minute), and low of 0 % (treatment 30 %, 15 minutes; and 10 %, 30 minutes); while soaking with redistilate highest attack 34,72 % (treatment of 2 %, 30 minutes), and low of 0 % (treatment of 10 %, 15 minutes; and 10 %, 30 minutes). At contamination average control of fungi attack is 80,85 %. The result of coconut oil showing that treatment applied in the redistilate liquid smoke from coconut shell with 10 % concentration and 30 minutes soaking time showed better result compared to control and other treatment with water content 2.45 %; free fatty acid contents 0.26 %; peroxide number 0.31 meg/kg; specifi c weight of 0.9130, refractive index 1.452, and color scale 0.7. Keywords: Liquid smoke, coconut shell, fungi, copra, coconut oil

The purposes of this research were to examine antioxidant activity, total phenolic content (TPC), total flavonoid content(TFC), and condensed tannin content (CTC) of blanced white saffron that were extracted with methanol : HCl=1000 :1.White saffron rhizomes were peeled, washed and blanched in the media of 0.0 5% and 0 % citric acid solution at 100 ºC for 5 and 10 minutes. �hite saffrons were evaluated antioxidant activity using 2-2-diphenyl-1-picrylhydrazyl (DPPH), TPC, TFC, and CTC. Blanched white saffron in the media of 0.05%, temperature 100 ºC for 5 minutes significantly increased the antioxidant activity (from 87.38 to 90.90 % RSA), TPC (from 58.35 to 81.80 mg GAE/g), TFC (from12.82 to 24.69 mg QE/g), and CTC (from 6.10 to 10.59 mg CE/g compare to the non-blanched. The antioxidant activ-ity of blanched white saffron has significantly positive correlation with TPC, TFC, and CTC. ABSTRAK Tujuan penelitian ini adalah mengetahui pengaruh blanching terhadap aktivitas antioksidan kadar fenol total, flavonoid total dan kadar tanin terkondensasi kunir putih yang diekstrak dengan pelarut metanol : HCl = 1000 : 1. Kunir putih dikupas, dicuci, dilakukan blanching dalam media asam sitrat 0,05 % dan 0 % (akuades), pada suhu 100 ºC selama5 dan 10 menit. Kunir putih diuji aktivitas antioksidan dengan metode 2-2-diphenyl-1-picrylhydrazyl (DPPH), kadarfenol total, flavonoid total dan tanin terkondensasi. Blanching dalam media asam sitrat 0,05 %, suhu 100 ºC selama5 menit dapat meningkatkan secara nyata aktivitas antioksidan dari 87,38 menjadi 90,90 % RSA (Radical Scaveng-ing Activity), kadar fenol total dari 58,35 menjadi 81,80 mg Ekivalen Asam Galat (EAG)/g, flavonoid total dari 12,82menjadi 24,69 mg ekivalen kuersetin (EK)/g dan tanin terkondensasi dari 6,10 menjadi 10,59 mg Ekivalen Catechin(EC)/g dibanding kunir putih tanpa blanching. Aktivitas antioksidan kunir putih yang telah mengalami blanching dalammedia asam sitrat 0 % maupun 0,05 % berkorelasi positif secara signifikan dengan kadar fenol total, flavonoid totaldan kadar tanin terkondensasi.

The objective of the present study was to determine the optimum condition of decaffeination process with chitosan in a model system using Response Surface Methodology. A 1000ppm caffeine solution was mixed with chitosan in varried concentrations, temperatures and process times. After filtration, caffeine in the filtrate was determined. The lower caffeine in the filtrate the more effective the decaffeination process. Result of the experiment showed that among chitosan concentrations of 50, 60, 70, 80, 90, and 100 mg per 100 ml caffeine solution, the concentration of 70mg was the most effective. Among temperatures applied of 28, 40, 60, 80, 90, and 100oC, the most effective was of 90oC. And among the process times of 15, 30, 60, and 90 minutes, 15 minutes was the most effective. Result of optimatization using RSM showed that the optimum condition of decaffeination process were concentration of chitosan of 69,52mg, temperature of 89,71oC, and process time of 14,88 minutes. Under this condition the process diminished 79,56% of caffeine from the model system.

Background : Torch ginger (Etlingera elatior) is an edible flower whose inflorescence is utlized for culinary purposes to enchance the taste of traditional dishes containing polyphenols and antioxidant compounds. However, investigation on the phytochemical profile and antioxidant activity of torch ginger inflorescence extract following simulated gastrointesinal digestion is still limited. Objective: This study aims to determine the phytochemical profile and evaluate the antioxidant activity of the inflorescence extract after in vitro simulated digestion. Methods: Torch ginger inflorescence (TGI) was extracted by ultrasound-assisted extraction with different solvents (water, 50%, and 80% aqueous ethanol). Total phenolics content (TPC), total flavonoid content (TFC), and antioxidant activity were determined by ORAC, DPPH, FRAP, and metal ion (Fe2+) chelating activity. The solvent extraction that gave the highest value of TPC, TFC, and antioxidant activities was used for an in vitro digestion and identification of phytochemicals profile by LC-ESI-QTOF-MS/MS. Furthermore, the TPC, TFC, and antioxidant capacities of digested TGI extract were compared to those of undigested extract. Results: TGI contained 90.24% moisture. The 80% aqueous ethanol extract exhibited the highest antioxidant capacity, with an ORAC value of 1,156.61 ± 11.55 mM TE/g extract, DPPH radical scavenging capacity of 1,087.68 ± 14.37 mM TE/g extract, FRAP value of 799.30 ± 1.45 mM TE/g extract, and Fe2+ chelating capacity of 42.32 ± 3.48% /mg extract. The 39 phytochemicals were identified for 9 flavonoids and 5 phenolic acids. The putative bioactive compounds for antioxidant, anticancer, anti-inflammatory, and anticholesterol were detected in TGIE, such as catechin, 2-hydroxycinnamic acid, astragalin, chlorogenic acid, coumarin, and procyanidin B2. After passing through an in vitro simulated mouth, gastric, and intestinal digestion, the TGI extract exhibited higher values of TPC, TFC, and antioxidative capacities than the undigested extract. Conclusion: This study reviewed the phytochemical components presented in the 80% aqueous ethanol TGIE. The expressed antioxidant capacity was increased when the TGIE passed through the in vitro simulated digestion, which could potentially represent a promising source of endogenous antioxidants in food and nutraceutical applications. [ABSTRACT FROM AUTHOR]