1. Zebrafish embryo extract counteracts human stem cell senescence
- Author
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Federica Facchin, Margherita Maioli, Umberto Santoro, Sara Santaniello, Eva Bianconi, Pier Mario Biava, Silvia Canaider, Valentina Basoli, Carlo Ventura, Facchin, Federica, Canaider, Silvia, Bianconi, Eva, Maioli, Margherita, Santoro, Umberto, Santaniello, Sara, Basoli, Valentina, Biava, Pier Mario, and Ventura, Carlo
- Subjects
Adult ,Cell Extracts ,0301 basic medicine ,Homeobox protein NANOG ,Senescence ,human Adipose-derived Stem Cell ,Telomerase ,Embryo, Nonmammalian ,Cell Survival ,Cellular differentiation ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Osteogenesis ,early developmental stage Zebrafish embryo extract ,Adipocytes ,Animals ,Humans ,Telomerase reverse transcriptase ,adipogenesi ,osteogenesis ,Cells, Cultured ,Cellular Senescence ,Cyclin-Dependent Kinase Inhibitor p16 ,Zebrafish ,Adipogenesis ,General Immunology and Microbiology ,Stem Cells ,senescence-associated β-galactosidase assay ,telomerase activity ,Cell Differentiation ,beta-Galactosidase ,Cell biology ,Transplantation ,030104 developmental biology ,cell senescence ,030220 oncology & carcinogenesis ,Female ,anti-senescence-associated gene expression ,Stem cell ,Stem Cell Transplantation ,Adult stem cell - Abstract
Human adult stem cells hold promise for regenerative medicine. They are usually expanded for multiple passages in vitro to increase cell yield prior to transplantation. Unfortunately, prolonged culture leads to cell senescence, a major drawback from successful outcomes in cell therapy approaches. Here, we show that an extract from early Zebrafish embryo (ZF1) counteracted senescence progression in human adipose-derived stem cells (hASCs) along multiple culture passages (from the 5th to the 20th). Exposure to ZF1 strongly reduced the expression of senescence marker beta-galactosidase. Both stemness (NANOG, OCT4, and MYC) and anti-senescence (BMI1, and telomerase reverse transcriptase - TERT) related genes were overexpressed at specific experimental points, without recruitment of the cyclin-dependent kinase Inhibitor 2A (CDKN2A, ali-as p16). Increased telomerase activity was associatt-ed with TERT overexpression. Both osteogenic and adipogenic abilities were enhanced. In conclusion, hASCs exposure to ZF1 is a feasible tool to counteract and reverse human stem cell senescence in long-term culturing conditions.
- Published
- 2019