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Your search keyword '"Unciti-broceta, Juan Diego"' showing total 11 results
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11 results on '"Unciti-broceta, Juan Diego"'

2. Neuroprotective Efficacy of Betulinic Acid Hydroxamate, a B55α/PP2A Activator, in Acute Hypoxia–Ischemia-Induced Brain Damage in Newborn Rats

Author

Silva, Laura, primary, Vargas, Carlos, additional, Prados, María Eugenia, additional, del Pozo, Aaron, additional, Villa, María, additional, Martínez, María, additional, Alvarez, Lourdes, additional, Muñoz, Eduardo, additional, Unciti-Broceta, Juan Diego, additional, and Martínez-Orgado, José, additional

Published
2022
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6. Drug 'Clicking' on Cell-Penetrating Fluorescent Nanoparticles for In Cellulo Chemical Proteomics

Author

Valero, Teresa, Delgado-gonzález, Antonio, Unciti-broceta, Juan Diego, Cano-cortés, Victoria, Pérez-lópez, Ana, Unciti-broceta, Asier, and Sánchez-martín, Rosario

Subjects
Proteomics, Azides, Cell Membrane Permeability, Cycloaddition Reaction, Drug-polymer conjugate, Dasatinib, Baeyer test, Catalysis, target ID, cell-penetrating nanoparticles, Humans, Nanoparticles, Click Chemistry, CuAAC, Fluorescent Dyes
Abstract

Chemical proteomics approaches are widely used to identify molecular targets of existing or novel drugs. This manuscript describes the development of a straightforward approach to conjugate azide-labelled drugs via click chemistryto alkyne-tagged cell-penetrating fluorescent nanoparticles as a novel tool to study target engagement and/or identification inside living cells. A modification of the Baeyer test for alkynes allows monitoring the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, guaranteeing the presence of the drug on the solid support. As a proof of concept, the conjugation of the promiscuous kinase inhibitor dasatinib to Cy5-labelled nanoparticles is presented. Dasatinib-decorated fluorescent nanoparticles efficiently inhibited its protein target SRC in vitro, entered cancer cells and colocalized with SRC in cellulo.

Published
2018
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9. New therapeutic approaches for human African trypanosomiasis

Author

Unciti-Broceta, Juan Diego, García-Salcedo, José Antonio, Universidad de Granada. Departamento de Bioquímica y Biología Molecular III e Inmunología, Consejo Superior de Investigaciones Científicas (CSIC). Instituto de Parasitología y Biomedicina López-Neyra, and García Salcedo, José Antonio

Subjects
Bioquímica, 616.9, 576.8, Parasitología, Trypanosoma brucei, Enfermedades infecciosas
Abstract

Proyecto FP7 Health-2007-2.3.4-1 "Nanotryp" (223048)., Tesis Univ. Granada. Departamento de Bioquímica y Biología Molecular III e Inmunología

Published
2014

10. Synthesis of 6,8,9 poly-substituted purine analogue libraries as pro-apoptotic inducers of human leukemic lymphocytes and DAPK-1 inhibitors

Author

Pineda de las Infantas, Maria J., primary, Torres-Rusillo, Sara, additional, Unciti-Broceta, Juan Diego, additional, Fernandez-Rubio, Pablo, additional, Luque-Gonzalez, Maria Angelica, additional, Gallo, Miguel A., additional, Unciti-Broceta, Asier, additional, Molina, Ignacio J., additional, and Diaz-Mochon, Juan J., additional

Published
2015
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11. Drug “Clicking” on Cell-Penetrating Fluorescent Nanoparticles for In CelluloChemical Proteomics

Author

Valero, Teresa, Delgado-González, Antonio, Unciti-Broceta, Juan Diego, Cano-Cortés, Victoria, Pérez-López, Ana M., Unciti-Broceta, Asier, and Sánchez Martín, Rosario M.

Abstract

Chemical proteomics approaches are widely used to identify molecular targets of existing or novel drugs. This manuscript describes the development of a straightforward approach to conjugate azide-labeled drugs via click chemistry to alkyne-tagged cell-penetrating fluorescent nanoparticles as a novel tool to study target engagement and/or identification inside living cells. A modification of the Baeyer test for alkynes allows monitoring the Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) reaction, guaranteeing the presence of the drug on the solid support. As a proof of concept, the conjugation of the promiscuous kinase inhibitor dasatinib to Cy5-labeled nanoparticles is presented. Dasatinib-decorated fluorescent nanoparticles efficiently inhibited its protein target SRC in vitro, entered cancer cells, and colocalized with SRC in cellulo.

Published
2018
Full Text
View/download PDF

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