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127 results on '"Univ. Valenciennes"'

1. Asymmetric micro-supercapacitors based on electrodeposited Ruo2 and sputtered VN films

Author: Christophe Lethien, Thierry Brousse, Kevin Robert, Bouchra Asbani, Pascal Roussel, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Réseau sur le stockage électrochimique de l'énergie (RS2E), Université de Nantes (UN)-Aix Marseille Université (AMU)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Université de Picardie Jules Verne (UPJV)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Circuits Systèmes Applications des Micro-ondes - IEMN (CSAM - IEMN ), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This research benefitted from the financial support of the ANR within the DENSSCAPIO project (ANR-17-CE05–0015–02). The authors would also like to thank the ANR STORE-EX and the RS2E for their financial support. The RENATECH network receives our utmost gratitude. Chevreul Institute (CNRS FR 2638), Ministère de l’Enseignement Supérieur et de la Recherche, Région Hauts de France and FEDER are acknowledged for supporting and funding XRD facilities., RS2E, Renatech Network, CMNF, ANR-17-CE05-0015,DENSSCAPIO,Supercondensateurs nanostructurés tout-solides pour stockage d'énergie plus dense et plus sûr(2017), ANR-10-LABX-0076,STORE-EX,Laboratory of excellency for electrochemical energy storage(2010), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Aix Marseille Université (AMU)-Université de Pau et des Pays de l'Adour (UPPA)-Université de Nantes (UN)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), CNRS, Centrale Lille, ENSCL, ISEN, Institut Catholique Lille, Univ. Artois, Univ. Valenciennes, Université de Lille, Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, and Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181
Subjects: Materials science, Vanadium nitride, Energy Engineering and Power Technology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, 7. Clean energy, Capacitance, Ruthenium oxide, vanadium nitride, chemistry.chemical_compound, asymmetric micro-supercapacitors, Sputtering, General Materials Science, Thin film, Electrochemical potential, Power density, Supercapacitor, Renewable Energy, Sustainability and the Environment, business.industry, [SPI.NRJ]Engineering Sciences [physics]/Electric power, hydrous ruthenium oxide, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, thin films, chemistry, electrodeposition, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Optoelectronics, sputtering, 0210 nano-technology, business
Abstract: International audience; Research on micro-supercapacitors remains one of the most important activities in the area of electrochemical energy storage for powering smart and miniaturized electronic devices. Here we design an asymmetric micro-supercapacitor (A-MSC) combining sputtered vanadium nitride film (VN) and electrodeposited hydrous ruthenium oxide (hRuO2) film. Taking advantage of their complementary electrochemical potential windows in 1 M KOH electrolyte, the A-MSC achieves a cell voltage up to 1.15 V, associated to high specific capacitance values for the device (≈ 100 mF cm−2). The energy density of this asymmetric VN / hRuO2 micro-supercapacitor reaches 20 µWh cm−2 at a power density of 3 mW cm−2. Taking benefit from the asymmetric configuration, a 5-fold enhancement factor in energy density is demonstrated for the VN / hRuO2 asymmetric micro-supercapacitor as compared to symmetric VN / VN and hRuO2 / hRuO2 microdevices.
Published: 2021
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2. A First Outlook of Sputtered FeWO4 Thin Films for Micro-Supercapacitor Electrodes

Author: Florent Blanchard, Gaëtan Buvat, Pascal Roussel, Thierry Brousse, Christophe Lethien, Antonella Iadecola, Réseau sur le stockage électrochimique de l'énergie (RS2E), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Aix Marseille Université (AMU)-Université de Pau et des Pays de l'Adour (UPPA)-Université de Nantes (UN)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, Institut des Matériaux Jean Rouxel (IMN), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN), Circuits Systèmes Applications des Micro-ondes - IEMN (CSAM - IEMN ), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Renatech Network, RS2E, ANR-10-LABX-0076,STORE-EX,Laboratory of excellency for electrochemical energy storage(2010), ANR-10-EQPX-0045,ROCK,Spectromètre EXAFS Rapide pour Cinétiques Chimiques(2010), CNRS, Centrale Lille, ENSCL, ISEN, Institut Catholique Lille, Univ. Artois, Univ. Valenciennes, Université de Lille, Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181, Université de Nantes (UN)-Aix Marseille Université (AMU)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Université de Picardie Jules Verne (UPJV)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-Université de Nantes (UN)-Ecole Polytechnique de l'Université de Nantes (EPUN), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)
Subjects: Fabrication, Materials science, 020209 energy, Oxide, 02 engineering and technology, 7. Clean energy, law.invention, chemistry.chemical_compound, law, 0202 electrical engineering, electronic engineering, information engineering, Materials Chemistry, Electrochemistry, Thin film, Supercapacitor, Renewable Energy, Sustainability and the Environment, business.industry, High voltage, [CHIM.MATE]Chemical Sciences/Material chemistry, Sputter deposition, Condensed Matter Physics, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Capacitor, chemistry, Electrode, Optoelectronics, business
Abstract: The next generation of Internet of Things devices requires micro-supercapacitors operating at high voltage which is difficult to achieve using symmetrical design. Thus, their fabrication in an asymmetric configuration is mandatory. While MnO 2 is well-established as positive electrode, the scarcity of existing efficient materials able to be used at the negative side drives the research towards new promising materials. Since few years, a new class of oxide materials, named multicationic oxides, were demonstrated to be attractive solutions as bulk electrodes for electrochemical capacitor. Among them, the wolframite-type FeWO 4 oxide was proposed as an interesting negative electrode material for asymmetric FeWO 4 /MnO 2 electrochemical capacitors. The present paper reports for the first time on the successful thin film synthesis of such iron-tungstate oxide films by reactive DC magnetron sputtering, a deposition method widely used in the semiconductor industry to manufacture micro-devices. The pseudocapacitive behaviour documented at the bulk scale is preserved at the thin film level as well, and opens-up the possibility to use FeWO 4 in the next generations of micro-supercapacitors. 168;3
Published: 2021
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3. Efficient reduction of Cr(VI) under visible light irradiation using CuS nanostructures

Author: Rabah Boukherroub, Yacine Cherifi, Nadia Aϊcha Laoufi, Alexandre Barras, Sawsen Nezar, Pascal Roussel, Nadia Saoula, Ahmed Addad, Sabine Szunerits, Elhadj Dogheche, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), University of Sciences and Technology Houari Boumediene [Alger] (USTHB), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), Optoélectronique - IEMN (OPTO - IEMN), Centre de Développement des Technologies Avancées (CDTA), Université Mouloud Mammeri [Tizi Ouzou] (UMMTO), Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, Centre National de la Recherche Scientifique (CNRS), the University of Lille, the Hauts-de-France region, Ministry of High Education and Scientific Research of Algeria, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Laboratoire de structures et propriétés de l'état solide (LSPES), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), Centre de Développement des Technologies Avancées (CTDA), Unité de Catalyse et de Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Ecole Centrale de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université des Sciences et de la Technologie Houari Boumediene = University of Sciences and Technology Houari Boumediene [Alger] (USTHB), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - Département Opto-Acousto-Électronique - UMR 8520 (IEMN-DOAE), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut National de la Recherche Agronomique (INRA), Université de Lille, Boukherroub, Rabah, ENSCL, Institut Catholique Lille, ISEN, Univ. Valenciennes, CNRS, INRA, Centrale Lille, Univ. Artois, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité Matériaux et Transformations - UMR 8207 [UMET], and Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Subjects: Photoreduction, Cr(VI), Formic acid, General Chemical Engineering, 02 engineering and technology, 010501 environmental sciences, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 01 natural sciences, lcsh:Chemistry, Absorbance, chemistry.chemical_compound, X-ray photoelectron spectroscopy, Spectrophotometry, medicine, Hexavalent chromium, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Visible light, CuS nanostructures, Adipic acid, medicine.diagnostic_test, Lake water, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 6. Clean water, Copper sulfide, lcsh:QD1-999, chemistry, Photocatalysis, 0210 nano-technology, Nuclear chemistry
Abstract: Photoreduction of hexavalent chromium, Cr(VI), identified as carcinogenic and mutagenic element, to Cr(III), believed to be an essential element, using copper sulfide nanostructures (CuS NSs) as photocatalyst was investigated under visible light irradiation (λ > 420 nm). The CuS NSs were synthesized at low temperature using a wet chemical route and fully characterized using various techniques such as scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy and UV–vis spectrophotometry. The photocatalytic reduction of Cr(VI) using visible light irradiation was assessed by UV–vis by following the decrease of the characteristic absorbance at ∼352 nm in the presence of CuS NSs. While CuS NSs promoted Cr(VI) full reduction within 60 min, addition of adipic acid or formic acid accelerated significantly the photocatalytic reduction process. Under optimized conditions, CuS NSs rapidly (12 min) reduced Cr(VI) in presence of 0.5 mM adipic acid. Similarly, Cu NSs were found to be efficient for Cr(VI) reduction dissolved in water from a local lake. Stability studies showed that the photocatalyst could be recycled up to four times without any apparent decrease of its catalytic performance. Keywords: CuS nanostructures, Cr(VI), Photoreduction, Visible light, Lake water
Published: 2019
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4. Plasmon-Induced Electrocatalysis with Multi-Component Nanostructures

Author: Robert Wojcieszak, Rabah Boukherroub, Sabine Szunerits, Franck Dumeignil, Dalila Meziane, Palaniappan Subramanian, Unité de Catalyse et Chimie du Solide - UMR 8181 (UCCS), Centrale Lille Institut (CLIL)-Université d'Artois (UA)-Centrale Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Université Mouloud Mammeri [Tizi Ouzou] (UMMTO), Unité de Catalyse et de Chimie du Solide - UMR 8181 (UCCS), Université d'Artois (UA)-Ecole Centrale de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Maison Univ, Inst Univ France, F-75005 Paris, Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), ENSCL, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Univ. Artois, Catholic University of Leuven - Katholieke Universiteit Leuven [KU Leuven], Université Mouloud Mammeri [Tizi Ouzou] [UMMTO], Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS], and Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Subjects: Materials science, Nanostructure, Nanotechnology, 02 engineering and technology, Review, fuel cells, engineering.material, 010402 general chemistry, Electrochemistry, Electrocatalyst, lcsh:Technology, 01 natural sciences, 7. Clean energy, plasmonics, Nanomaterials, Catalysis, [CHIM]Chemical Sciences, General Materials Science, catalysis, nanomaterials, electrochemistry, fuel, lcsh:Microscopy, Plasmon, ComputingMilieux_MISCELLANEOUS, lcsh:QC120-168.85, lcsh:QH201-278.5, lcsh:T, [CHIM.CATA]Chemical Sciences/Catalysis, 021001 nanoscience & nanotechnology, 0104 chemical sciences, lcsh:TA1-2040, engineering, lcsh:Descriptive and experimental mechanics, Noble metal, Charge carrier, lcsh:Electrical engineering. Electronics. Nuclear engineering, lcsh:Engineering (General). Civil engineering (General), 0210 nano-technology, lcsh:TK1-9971
Abstract: International audience; Noble metal nanostructures are exceptional light absorbing systems, in which electron–hole pairs can be formed and used as “hot” charge carriers for catalytic applications. The main goal of the emerging field of plasmon-induced catalysis is to design a novel way of finely tuning the activity and selectivity of heterogeneous catalysts. The designed strategies for the preparation of plasmonic nanomaterials for catalytic systems are highly crucial to achieve improvement in the performance of targeted catalytic reactions and processes. While there is a growing number of composite materials for photochemical processes-mediated by hot charge carriers, the reports on plasmon-enhanced electrochemical catalysis and their investigated reactions are still scarce. This review provides a brief overview of the current understanding of the charge flow within plasmon-enhanced electrochemically active nanostructures and their synthetic methods. It is intended to shed light on the recent progress achieved in the synthesis of multi-component nanostructures, in particular for the plasmon-mediated electrocatalysis of major fuel-forming and fuel cell reactions.
Published: 2019
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5. A Novel Integrated Way for Deciphering the Glycan Code for the FimH Lectin

Author: Dumych, Tetiana, Bridot, Clarisse, Gouin, Sebastien, Lensink, Marc, Paryzhak, Solomiya, Szunerits, Sabine, Blossey, Ralf, Bilyy, Rostyslav, Bouckaert, Julie, Krammer, Eva-Maria, Danylo Halytskiy Lviv National Medical University, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), CNRS, UMR 8576, Université de Lille, Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire de Synthèse Organique (Hétérochimie organique, organoéléments et matériaux) (LSOHOOM), Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Ecole Centrale de Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Ecole Centrale de Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Institut de Recherche Interdisciplinaire,FRE 2963 (IRI), Université de Lille, Sciences et Technologies-Institut d'Electronique ,Microélectronique et Nanotechnologies-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), This research has received funding from a grant from the Ministry of Healthcare of Ukraine project 0116U000759 (Rostyslav Bilyy). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No H2020-MSCA-RISE-2015, PANG-690836 (S.S.), and agreement No. 750280 (E.-M.K.)., Computational resources have been provided by the Centre de Ressources Informatiques (CRI) of the University Lille 1 Sciences et Technologies., ISEN, Univ. Valenciennes, CNRS, Institut Catholique Lille, Centrale Lille, Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Ivan Franko National University of Lviv, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], and Danylo Halytsky Lviv National Medical University [Lviv, Ukraine]
Subjects: Models, Molecular, [SDV]Life Sciences [q-bio], Manα1,3Man, Molecular Conformation, binding mode, Calorimetry, high-mannose N-glycan, Bacterial Adhesion, Article, lcsh:QD241-441, thermodynamics, lcsh:Organic chemistry, Polysaccharides, FimH, Lectins, parasitic diseases, Enzyme-Linked LectinoSorbent assay, Escherichia coli, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Adhesins, Escherichia coli, Manα1,2Man, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, microcalorimetry, molecular dynamics, carbohydrates (lipids), [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, bacteria, Fimbriae Proteins, entropy, Mannose, Protein Binding
Abstract: The fimbrial lectin FimH from uro- and enteropathogenic Escherichia coli binds with nanomolar affinity to oligomannose glycans exposing Man&alpha, 1,3Man dimannosides at their non-reducing end, but only with micromolar affinities to Man&alpha, 1,2Man dimannosides. These two dimannoses play a significantly distinct role in infection by E. coli. Man&alpha, 1,2Man has been described early on as shielding the (Man&alpha, 1,3Man) glycan that is more relevant to strong bacterial adhesion and invasion. We quantified the binding of the two dimannoses (Man&alpha, 1,2Man and Man&alpha, 1,3Man to FimH using ELLSA and isothermal microcalorimetry and calculated probabilities of binding modes using molecular dynamics simulations. Our experimentally and computationally determined binding energies confirm a higher affinity of FimH towards the dimannose Man&alpha, 1,3Man. Man&alpha, 1,2Man displays a much lower binding enthalpy combined with a high entropic gain. Most remarkably, our molecular dynamics simulations indicate that Man&alpha, 1,2Man cannot easily take its major conformer from water into the FimH binding site and that FimH is interacting with two very different conformers of Man&alpha, 1,2Man that occupy 42% and 28% respectively of conformational space. The finding that Man&alpha, 1,2Man binding to FimH is unstable agrees with the earlier suggestion that E. coli may use the Man&alpha, 1,2Man epitope for transient tethering along cell surfaces in order to enhance dispersion of the infection.
Published: 2018
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6. Atmospheric pressure plasma spraying of silane-based coatings targeting whey protein fouling and bacterial adhesion management

Author: Yannick Coffinier, Maude Jimenez, Christophe Andre, Séverine Bellayer, Guillaume Delaplace, Nour-Eddine Chihib, Thierry Six, Sawsen Zouaghi, Marwan Abdallah, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), Université de Lille, Sciences et Technologies-École polytechnique universitaire de Lille (Polytech Lille), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génie des Procédés et Technologie Alimentaires (LGPTA), Institut National de la Recherche Agronomique (INRA), ECONOMICS project (ANR-17-CE08-0032), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE08-0032,ECONOMICS,Design de surfaces anti-encrassantes pour les industries agroalimentaires(2017), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, ENSCL, Institut Catholique Lille, ISEN, Univ. Valenciennes, CNRS, INRA, Centrale Lille, and Unité Matériaux et Transformations - UMR 8207 [UMET]
Subjects: Whey protein, Materials science, Scanning electron microscope, Dairy pasteurization, General Physics and Astronomy, Atmospheric-pressure plasma, 02 engineering and technology, engineering.material, Biofouling, chemistry.chemical_compound, [SPI]Engineering Sciences [physics], 0404 agricultural biotechnology, Coating, Silane, HMDSO, ComputingMilieux_MISCELLANEOUS, Fouling, [SDE.IE]Environmental Sciences/Environmental Engineering, technology, industry, and agriculture, 04 agricultural and veterinary sciences, Surfaces and Interfaces, General Chemistry, Adhesion, Antifouling, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 040401 food science, Surfaces, Coatings and Films, chemistry, Chemical engineering, engineering, Atmospheric pressure plasma spraying, 0210 nano-technology
Abstract: International audience; This work aims at studying the impact of silane-based coatings, generated by atmospheric pressure plasma spraying (APPS) of a liquid precursor (hexamethydisiloxane – HMDSO), on dairy antifouling and antibacterial properties of food-grade 316L stainless steel. The influence of the manufacturing parameters (precursor flow rate, nozzle-to-substrate distance and scanning speed) on the coatings properties was investigated using a wide range of characterization techniques (drop shape analysis, X-Ray Mappings, Scanning Electron Microscopy, Fourier-Transformed Infrared Spectroscopy, Atomic Force Microscopy). Coating’s roughness in particular was shown to strongly increase when precursor flow rate increases. A pilot pasteurizer, fed with a model foulant solution (whey protein and calcium), allowed performing two consecutive industrial-like isothermal dairy fouling tests, revealing the promising anti-fouling properties of the HMDSO-coated steel. A fouling reduction of up to 90% compared to bare stainless steel was achieved after first cycle for all samples. The second fouling run allowed to select the best-performing sample, which kept the same antifouling properties as in the first test. Its mechanism of action was investigated, which revealed that a nanostructured, Si-O-Si rich surface was efficient to prevent isothermal dairy fouling. The adhesion of the pathogenic bacterium Staphylococcus aureus also proved to be impacted by this plasma coating, with a significant decrease of adhered cells (−30% compared to native stainless steel).
Published: 2018
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7. Reduced graphene oxide/polyethylenimine based immunosensor for the selective and sensitive electrochemical detection of uropathogenic Escherichia coli

Author: Karima Kahlouche, Roxana Jijie, Rabah Boukherroub, Sabine Szunerits, Julie Bouckaert, Alexandre Barras, Tijani Gharbi, Nao Yamakawa, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Nanomédecine, imagerie, thérapeutique - UFC (UR 4662) [NIT / NANOMEDECINE], Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Franche-Comté (UFC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Technologie de Belfort-Montbeliard (UTBM), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Subjects: Electrophoretic deposition, Reduced graphene oxide, Polyethyleneimine, Anti- fimbrial E. coli antibodies, E. coli UTI89 bacteria, Electrochemical sensing, 02 engineering and technology, medicine.disease_cause, 01 natural sciences, law.invention, chemistry.chemical_compound, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Materials Chemistry, medicine, Electrical and Electronic Engineering, Instrumentation, Escherichia coli, ComputingMilieux_MISCELLANEOUS, Detection limit, Polyethylenimine, Aqueous solution, Chemistry, Potassium ferrocyanide, Graphene, 010401 analytical chemistry, Metals and Alloys, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Combinatorial chemistry, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Covalent bond, Surface modification, 0210 nano-technology
Abstract: Fast, reliable and selective detection of microorganisms is of uttermost importance in clinical analysis, but also in food and water quality monitoring. In this study, we report on the construction of an immunosensor for sensitive and selective electrochemical detection of uropathogenic Escherichia coli (E. coli) UTI89 bacteria in aqueous and serum samples. We took benefit of electrophoretic deposition (EPD) to prepare, in a simple, controllable and cost effective way, gold electrodes modified with thin active layers of reduced graphene oxide/polyethylenimine (rGO/PEI). While rGO exhibits high surface area and favourable electrochemical properties, the presence of abundant single bondNH2 groups on PEI offers a plethora of opportunities for the sensor’s surface functionalization. To achieve selectivity of detection, the electrode surface was covalently modified with anti-fimbrial E. coli antibodies via amide bond formation. To minimize non-specific adsorption, the immunosensor was additionally modified with pyrene-polyethyleneglycol (pyrene-PEG) moieties prior to antibody immobilization. The detection of E. coli was based on the restriction of electron transfer of a redox mediator, in our case potassium ferrocyanide, to the rGO/PEI modified electrical transducer due to the formation of an immune complex. The developed immunosensor displayed a sigmoidal shape with a linear range of 1 × 101–1 × 104 cfu mL−1 (R2 = 0.995) according to i(μA) = −16.66–20.5 × log[E. coli] (cfu mL−1) and a detection limit of 10 cfu mL−1. Additionally, the sensor performed well both in aqueous, serum and urine media, which is essential for its potential use for clinical diagnosis of pathogenic diseases. Selectivity studies showed that the immunosensor was able to discriminate between E. coli UTI89 wild-type strain and UTI89 Δfim, without fim operon. 260
Published: 2018
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8. Kinetic proofreading of chromatin remodeling: from gene activation to gene repression and back

Author: Raghvendra P Singh, Ralf Blossey, Fabrizio Cleri, Marc F. Lensink, Guillaume Brysbaert, ISEN, Univ. Valenciennes, CNRS, Institut Catholique Lille, Centrale Lille, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, "null", Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: Biophysics, histone, Biochemistry, Chromatin remodeling, 03 medical and health sciences, Structural Biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], bromodomain, Histone methylation, Histone code, Nucleosome, lcsh:QH301-705.5, Molecular Biology, chromatin, histone code, remodeler, nucleosome, 030304 developmental biology, Regulation of gene expression, Genetics, 0303 health sciences, biology, 030302 biochemistry & molecular biology, Chromatin, Cell biology, enzymes and coenzymes (carbohydrates), Histone, lcsh:Biology (General), biology.protein, Kinetic proofreading
Abstract: International audience; ATP-dependent chromatin remodeling is the active displacement of nucleosomes along or off DNA induced by chromatin remodeling complexes. This key process of gene regulation in eukaryote organisms has recently been argued to be controlled by a kinetic proofreading mechanism. In this paper we present a discussion of the current understanding of this process. We review the case of gene repression via heterochromatin formation by remodelers from the ISWI family and then discuss the activation of the IFN-β gene, where the displacement of the nucleosome is initiated by histone tail acetylations by the enzyme GCN5 which are required for the recruitment of SWI-SNF remodelers. We quantify the speci city of the acetylation step in the remodeling process by peptide docking simulations.
Published: 2015
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9. Comparison of Ti-Based Coatings on Silicon Nanowires for Phosphopeptide Enrichment and Their Laser Assisted Desorption/Ionization Mass Spectrometry Detection

Author: Kurylo, I. (Ievgen), Hamdi, A. (Abderrahmane), Addad, A. (Ahmed), Boukherroub, R. (Rabah), Coffinier, Y. (Yannick), Université de Lille, ISEN, Univ. Valenciennes, ENSCL, CNRS, INRA, Institut Catholique Lille, Centrale Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], and Unité Matériaux et Transformations - UMR 8207 [UMET]
Subjects: 0301 basic medicine, Materials science, General Chemical Engineering, Analytical chemistry, engineering.material, LDI-MS, Mass spectrometry, 01 natural sciences, Article, law.invention, lcsh:Chemistry, 03 medical and health sciences, Atomic layer deposition, X-ray photoelectron spectroscopy, Coating, law, Desorption, titania, General Materials Science, Spectroscopy, silicon nanowires, phosphopeptides, serum, Phosphopeptide, 010401 analytical chemistry, Laser, 0104 chemical sciences, 030104 developmental biology, lcsh:QD1-999, engineering
Abstract: We created different TiO2-based coatings on silicon nanowires (SiNWs) by using either thermal metallization or atomic layer deposition (ALD). The fabricated surfaces were characterized by X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDX), and reflectivity measurements. Surfaces with different TiO2 based coating thicknesses were then used for phosphopeptide enrichment and subsequent detection by laser desorption/ionization mass spectrometry (LDI-MS). Results showed that the best enrichment and LDI-MS detection were obtained using the silicon nanowires covered with 10 nm of oxidized Ti deposited by means of thermal evaporation. This sample was also able to perform phosphopeptide enrichment and MS detection from serum. 7
Published: 2017

10. On demand electrochemical release of drugs from porous reduced graphene oxide modified flexible electrodes

Author: Samia Boulahneche, Roxana Jijie, Sabine Szunerits, Sreekumar Kurungot, Julie Bouckaert, Rabah Boukherroub, Santosh K. Singh, Fereshteh Chekin, Alexandre Barras, Mohamed Salah Medjram, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Islamic Azad University, Amol, CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université 20 Août 1955 Skikda, Financial support from the Centre National de la Recherche Scientifique (CNRS), the University Lille 1, the Hauts-de-France region, the CPER 'Photonics for Society', the Agence National de la Recherche (ANR) and the EU union through FLAG-ERA JTC 2015-Graphtivity, and the Marie Sklodowska-Curie action (H2020-MSCA-RISE-2015, PANG-690836) are acknowledged. Samia Boulahneche thanks the Algerian Government for a PhD scholarship., European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Faculté des Sciences [Skikda], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Islamic Azad University, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Faculté de Technologie [Skikda], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), and Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
Subjects: Drug, Materials science, media_common.quotation_subject, Biomedical Engineering, Oxide, Nanotechnology, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, Electrochemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, law, Ampicillin, medicine, General Materials Science, media_common, Ondansetron hydrochloride, Graphene, General Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Antimicrobial, Combinatorial chemistry, 0104 chemical sciences, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, chemistry, Electrode, 0210 nano-technology, medicine.drug
Abstract: International audience; Despite the advantages of an electrochemical control for drug release, only a handful of electrochemical-based release systems have been developed so far. We report herein on the development of an electrochemically activatable platform for on-demand delivery of drugs. It is based on flexible gold thin film electrodes coated with porous reduced graphene oxide (prGO) nanosheets onto which the drug of interest has been integrated beforehand. Two different drugs are investigated here: ondansetron hydrochloride (ODS), a selective 5-HT3 receptor antagonist used for preventing nausea and vomiting caused by chemotherapy and radiotherapy, and ampicillin (AMP), an antibiotic to prevent and treat a number of bacterial infections such as respiratory tract infections, urinary tract infections, and meningitis. In the case of ODS, application of a negative potential bias of −0.8 V results in a sustained slow ODS release with an ODS flux of 47 μg cm−2 h−1. In the case of AMP, we show that polyethyleneimine modified prGO (prGO/PEI) is an extremely efficient matrix. Upon the application of +0.8 V, 24% of AMP could be released from the electrical interface in a time span of 2 h. The released AMP kept its antibacterial activity as demonstrated by antimicrobial tests. These examples illustrate the major benefits of the developed approach for biomedical applications.
Published: 2017
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11. Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters

Author: Santosh K. Singh, Roxana Jijie, Fatima Halouane, Sabine Szunerits, Sreekumar Kurungot, Jean Jurazek, Rabah Boukherroub, Dalila Meziane, Julie Bouckaert, Chengnan Li, Alexandre Barras, Musen Li, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Université Mouloud Mammeri [Tizi Ouzou] (UMMTO), CSIR-National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Groupe de physique des matériaux (GPM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Shandong University, European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche sur les Matériaux Avancés (IRMA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Université Mouloud Mammeri [Tizi Ouzou] [UMMTO], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], and Groupe de physique des matériaux [GPM]
Subjects: Materials science, Biomedical Engineering, 02 engineering and technology, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Microbiology, chemistry.chemical_compound, medicine, General Materials Science, Pathogen, Body fluid, biology, Pathogenic bacteria, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, chemistry, Surface modification, Magnetic nanoparticles, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Nanocarriers, 0210 nano-technology, Ethylene glycol, Bacteria
Abstract: International audience; The fast and efficient elimination of pathogenic bacteria from water, food or biological samples such as blood remains a challenging task. Magnetic isolation of bacteria from complex media holds particular promise for water disinfection and other biotechnological applications employing bacteria. When it comes to infectious diseases such as urinary tract infections, the selective removal of the pathogenic species in complex media such as human serum is also of importance. This issue can only be accomplished by adding pathogen specific targeting sites onto the magnetic nanostructures. In this work, we investigate the potential of 2-nitrodopamine modified magnetic particles anchored on reduced graphene oxide (rGO) nanocomposites for rapid capture and efficient elimination of E. coli associated with urinary tract infections (UTIs) from water and serum samples. An optimized magnetic nanocarrier achieves a 99.9% capture efficiency even at E. coli concentrations of 1 × 10 1 cfu mL −1 in 30 min. In addition, functionalization of the nanostructures with poly(ethylene glycol) modified pyrene units and anti-fimbrial E. coli antibodies allowed specific elimination of E. coli UTI89 from serum samples. Irradiation of the E. coli loaded nanocomposite with a near-infrared laser results in the total ablation of the captured pathogens. This method can be flexibly modified for any other pathogenic bacteria, depending on the antibodies used, and might be an interesting alternative material for a magnetic-based body fluid purification approach.
Published: 2017
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12. On the ability of molecular dynamics simulation and continuum electrostatics to treat interfacial water molecules in protein-protein complexes

Author: Guillaume Copie, Ralf Blossey, Fabrizio Cleri, Marc F. Lensink, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), ISEN, Univ. Valenciennes, CNRS, Institut Catholique Lille, Centrale Lille, Université de Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], and Université de Lille-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Barnase, Multidisciplinary, biology, Chemistry, Protein domain, Protein Data Bank (RCSB PDB), [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Molecular Dynamics Simulation, Electrostatics, Article, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, 03 medical and health sciences, Molecular dynamics, 030104 developmental biology, Protein Domains, Chemical physics, Multiprotein Complexes, Colicin, biology.protein, Molecule, Barstar, Databases, Protein
Abstract: Interfacial waters are increasingly appreciated as playing a key role in protein-protein interactions. We report on a study of the prediction of interfacial water positions by both Molecular Dynamics and explicit solvent-continuum electrostatics based on the Dipolar Poisson-Boltzmann Langevin (DPBL) model, for three test cases: (i) the barnase/barstar complex (ii) the complex between the DNase domain of colicin E2 and its cognate Im2 immunity protein and (iii) the highly unusual anti-freeze protein Maxi which contains a large number of waters in its interior. We characterize the waters at the interface and in the core of the Maxi protein by the statistics of correctly predicted positions with respect to crystallographic water positions in the PDB files as well as the dynamic measures of diffusion constants and position lifetimes. Our approach provides a methodology for the evaluation of predicted interfacial water positions through an investigation of water-mediated inter-chain contacts. While our results show satisfactory behaviour for molecular dynamics simulation, they also highlight the need for improvement of continuum methods.
Published: 2016
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13. Non-Enzymatic Glucose Sensing Using Carbon Quantum Dots Decorated with Copper Oxide Nanoparticles

Author: Florina Teodoresu, Rabah Boukherroub, Guohui Pan, Houcem Maaoui, Sabine Szunerits, Radhouane Chtourou, Ahmed Addad, Qian Wang, Université de Lille, ISEN, Univ. Valenciennes, ENSCL, CNRS, INRA, Institut Catholique Lille, Centrale Lille, Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Changchun Institute of Technology, Unité Matériaux et Transformations (UMET) - UMR 8207, Université virtuelle de Tunis [UVT], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Unité Matériaux et Transformations - UMR 8207 (UMET), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Université virtuelle de Tunis (UVT)
Subjects: Copper oxide, Nanostructure, Glucose sensing, non-enzymatic, Nanotechnology, 02 engineering and technology, 010402 general chemistry, Electrochemistry, lcsh:Chemical technology, 01 natural sciences, Biochemistry, Article, Analytical Chemistry, chemistry.chemical_compound, [CHIM]Chemical Sciences, Glucose homeostasis, lcsh:TP1-1185, Electrical and Electronic Engineering, Instrumentation, Detection limit, glucose sensing, copper oxide nanoparticles, 021001 nanoscience & nanotechnology, carbon quantum dots, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, chemistry, electrochemistry, Carbon quantum dots, serum, 0210 nano-technology, Fluorescent glucose biosensor, Nuclear chemistry
Abstract: International audience; Perturbations in glucose homeostasis is critical for human health, as hyperglycemia (defining diabetes) leads to premature death caused by macrovascular and microvascular complications. However, the simple and accurate detection of glucose in the blood at low cost remains a challenging task, although it is of great importance for the diagnosis and therapy of diabetic patients. In this work, carbon quantum dots decorated with copper oxide nanostructures (CQDs/Cu2O) are prepared by a simple hydrothermal approach, and their potential for electrochemical non-enzymatic glucose sensing is evaluated. The proposed sensor exhibits excellent electrocatalytic activity towards glucose oxidation in alkaline solutions. The glucose sensor is characterized by a wide concentration range from 6 µM to 6 mM, a sensitivity of 2.9 ± 0.2 µA·µM−1·cm−2, and a detection limit of 6 µM at a signal-to-noise ratio S/N = 3. The sensors are successfully applied for glucose determination in human serum samples, demonstrating that the CQDs/Cu2O-based glucose sensor satisfies the requirements of complex sample detection with adapted potential for therapeutic diagnostics
Published: 2016
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14. Differentiation of Crohn’s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force

Author: Amaia Pesquera, Oleksandr Zagorodko, Nicolas Barnich, Tetiana Dumych, Amaia Zurutuza, Virginie Cogez, Anne Harduin-Lepers, Sébastien G. Gouin, Thibaut Chalopin, Sabine Szunerits, Aritz Yanguas Serrano, Aloysius Siriwardena, Adeline Sivignon, Julie Bouckaert, Rabah Boukherroub, Iban Larroulet, Dimitri Alvarez Dorta, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM], Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte [M2iSH], Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 [LG2A ], Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Département Microbiologie et Chaîne Alimentaire (MICA), Institut National de la Recherche Agronomique (INRA), Laboratoire des Glucides (LG), Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé-Université de Lille, Sciences et Technologies, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), and Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Fimbria, heptyl α-D-mannose, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, shear force, Microbiology, 03 medical and health sciences, Pathogenic Escherichia coli, Escherichia, medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Escherichia coli, General Immunology and Microbiology, biology, graphene, Lectin, Adhesion, biology.organism_classification, Bacterial adhesin, 030104 developmental biology, adherent-invasive Escherichia coli, Surface Plasmon Resonance, heptyl α-d-mannose, Pilin, biology.protein, surface plasmon resonance, heptyl α-, d<%2Fspan>-mannose%22">">d-mannose, General Agricultural and Biological Sciences
Abstract: International audience; Shear force exerted on uropathogenic Escherichia coli adhering to surfaces makes type-1 fimbriae stretch out like springs to catch on to mannosidic receptors. This mechanism is initiated by a disruption of the quaternary interactions between the lectin and the pilin of the two-domain FimH adhesin and transduces allosterically to the mannose-binding pocket of FimH to increase its affinity. Mannose-specific adhesion of 14 E. coli pathovars was measured under flow, using surface plasmon resonance detection on functionalized graphene-coated gold interfaces. Increasing the shear had important differential consequences on bacterial adhesion. Adherent-invasive E. coli, isolated from the feces and biopsies of Crohn's disease patients, consistently changed their adhesion behavior less under shear and displayed lower SPR signals, compared to E. coli opportunistically infecting the urinary tract, intestines or loci of knee and hip prostheses. We exemplified this further with the extreme behaviors of the reference strains UTI89 and LF82. Whereas their FimA major pilins have identical sequences, FimH of LF82 E. coli is marked by the Thr158Pro mutation. Positioned in the inter-domain region known to carry hot spots of mutations in E. coli pathotypes, residue 158 is indicated to play a structural role in the allosteric regulation of type-1 fimbriae-mediated bacterial adhesion.
Published: 2016
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15. Affinity of Glycan-Modified Nanodiamonds towards Lectins and Uropathogenic Escherichia Coli

Author: Vladimir Zaitsev, Alexandre Barras, Aloysius Siriwardena, Julie Bouckaert, Rostyslav Bilyy, Volodymyr Turcheniuk, Rabah Boukherroub, Tetiana Dumych, Kostiantyn Turcheniuk, Sabine Szunerits, Qi Wang, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, Centrale Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Danylo Halytsky Lviv National Medical University [Lviv, Ukraine], Taras Shevchenko National University of Kyiv, Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 [LG2A ], Shandong University, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Droit et Santé, Danylo Halytskiy Lviv National Medical University, Institute of Applied Physics of RAS, Russian Academy of Sciences [Moscow] (RAS), Laboratoire de Glycochimie, des antimicrobiens et des agro-ressources (LG2A ), Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), and Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Glycan, Materials science, Affinity, Escherichia coli, Glycans, Nanodiamonds, Photo-coupling, Energy Engineering and Power Technology, Mannose, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Biomaterials, chemistry.chemical_compound, Materials Chemistry, medicine, Monosaccharide, Nanodiamond, Binding affinities, chemistry.chemical_classification, biology, Renewable Energy, Sustainability and the Environment, Biofilm, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Biochemistry, Covalent bond, biology.protein, 0210 nano-technology
Abstract: International audience; Nanodiamond particles (NDs) modified with glycan ligands are revealing themselves to have great promise as new nanomaterials for combating biofilm formation and as promising anti-adhesive scaffolds. Currently, the strategies at hand to formulate glycan-modified NDs (glyco-NDs) are limited to a few reports. We demonstrate herein that the photoinduced covalent attachment of unmodified sugars results in glyco-NDs with high binding affinity to lectins and a uropathogenic Escherichia coli strain (E. coli UTI89). While the binding affinities of glyco-NDs to different lectins is partially sacrificed when monosaccharides such as mannose are photochemically integrated onto NDs, in the case of disac-charides and oligosaccharides the binding affinity of glyco-NDs to lectins is preserved. Moreover, mannan-modified NDs show strong interactions with uropathogenic E. coli., suggesting the effectiveness of photochemically formed glyco-NDs for disruption of E. coli-mediated biofilms.
Published: 2016
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16. Plasmonic photothermal cancer therapy with gold nanorods/reduced graphene oxide core/shell nanocomposites

Author: Julie Bouckaert, Rabah Boukherroub, Volodymyr Vovk, Natasha Prevarskaya, Rostyslav Bilyy, Kostiantyn Turcheniuk, Tetiana Dumych, Valentyna Chopyak, Pascal Mariot, Vladimir Zaitsev, Sabine Szunerits, Volodymyr Turcheniuk, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Danylo Halytskiy Lviv National Medical University, Institute of Applied Physics of RAS, Russian Academy of Sciences [Moscow] (RAS), Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate, Université de Lille, Sciences et Technologies-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie Cellulaire (PHYCEL) - U1003 (PHYCEL), Université Lille Nord de France (COMUE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie Cellulaire : Canaux ioniques, inflammation et cancer - U 1003 (PHYCELL), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, ISEN, Univ. Valenciennes, CNRS, Inserm, Institut Catholique Lille, Centrale Lille, Université de Lille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Danylo Halytsky Lviv National Medical University [Lviv, Ukraine], Taras Shevchenko National University of Kyiv, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Laboratoire de Physiologie Cellulaire : Canaux ioniques, inflammation et cancer - U 1003 [PHYCELL], Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Feret, Romain, and Pathogen and Graphene - PANG - - H20202016-01-01 - 2019-12-31 - 690836 - VALID
Subjects: Materials science, General Chemical Engineering, [CHIM.THER] Chemical Sciences/Medicinal Chemistry, Nanotechnology, [SDV.CAN]Life Sciences [q-bio]/Cancer, 02 engineering and technology, Polyethylene glycol, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, 01 natural sciences, law.invention, Nanomaterials, chemistry.chemical_compound, [SDV.CAN] Life Sciences [q-bio]/Cancer, In vivo, law, Irradiation, Graphene, General Chemistry, Photothermal therapy, 021001 nanoscience & nanotechnology, Fluorescence, 3. Good health, 0104 chemical sciences, chemistry, Nanorod, 0210 nano-technology
Abstract: International audience; Gold nanorods (Au NRs) are known for their efficient conversion of photon energy into heat, resulting in hyperthermia and suppression of tumor growth in vitro and in vivo. Au NRs are thus of great promise for photothermal therapy (PTT) of different cancers. From the point of cancer therapy, low laser powers are essential (≤1 W cm −2) to ensure minimal side effects such as skin burning. Herein, we investigate the potential of polyethylene glycol functionalized reduced graphene oxide (rGO-PEG) enrobed Au NRs for the photothermal destruction of human glioblastoma astrocytoma (U87MG) cells in mice. We show that Au NRs@rGO-PEG are ideal multifunctional theranostic nanostructures that can exert efficient photothermal destruction of tumors in mice upon low doses of NIR light excitation and can act as fluorescent cellular markers due to the presence of a NIR dye integrated onto the rGO shell. Due to the specific interaction between Tat protein modified Au NRs@rGO-PEG nanostructures with the human glioblastoma astrocytoma (U87MG) cells, selective targeting of the tumor is achieved. In vivo experiments in mice show that upon irradiation of the tumor implanted in mice at 800 nm under low doses (0.7 W cm −2), U87MG tumor growth gets suppressed. The study demonstrates that the novel nanomaterials allow for an efficient destruction of solid tumors and might thus serve as an excellent multi-functional theranostic agent in photothermal therapeutic applications.
Published: 2016
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17. Flexible Nanoholey Patches for Antibiotic-Free Treatments of Skin Infections

Author: Solomiya Paryzhak, Julie Bouckaert, Volodymyr Vovk, Chengnan Li, Rabah Boukherroub, Rostyslav Bilyy, Amaia Zurutuza, Ran Ye, Sorin Melinte, Tetiana Dumych, Sabine Szunerits, Musen Li, Djamel Drider, Institut d’Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520 (IEMN), Centre National de la Recherche Scientifique (CNRS)-Université de Lille-Université Polytechnique Hauts-de-France (UPHF)-Ecole Centrale de Lille-Université Polytechnique Hauts-de-France (UPHF)-Institut supérieur de l'électronique et du numérique (ISEN), Shandong University, Université Catholique de Louvain (UCL), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Institut Charles Viollette (ICV) - EA 7394 (ICV), Université d'Artois (UA)-Institut National de la Recherche Agronomique (INRA)-Université du Littoral Côte d'Opale (ULCO)-Institut Supérieur d'Agriculture-Université de Lille, Danylo Halytskiy Lviv National Medical University, European Project: 690836,H2020,H2020-MSCA-RISE-2015,PANG(2016), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Université Catholique de Louvain = Catholic University of Louvain (UCL), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de la Recherche Agronomique (INRA)-Université d'Artois (UA)-Institut Supérieur d'Agriculture, Institut Catholique Lille, Université de Lille, ISEN, Univ. Valenciennes, CNRS, INRA, ISA, Centrale Lille, Univ. Artois, Univ. Littoral Côte d’Opale, Institut Charles Viollette (ICV) - EA 7394, Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Université Catholique de Louvain = Catholic University of Louvain [UCL], Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Institut Charles Viollette (ICV) - ULR 7394, and Danylo Halytsky Lviv National Medical University [Lviv, Ukraine]
Subjects: Exudate, Materials science, photothermal therapy, medicine.drug_class, Antibiotics, polyimide, reduced graphene oxide, wound infection, bacteria ablation, in vivo treatment, gold nanoholes, Nanotechnology, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Skin infection, 010402 general chemistry, 01 natural sciences, Microbiology, Mice, In vivo, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, medicine, Animals, General Materials Science, integumentary system, Oxides, Penetration (firestop), Bacterial Infections, [CHIM.MATE]Chemical Sciences/Material chemistry, Photothermal therapy, 021001 nanoscience & nanotechnology, medicine.disease, 0104 chemical sciences, 3. Good health, Skin patch, Anti-Bacterial Agents, Nanostructures, Graphite, Gold, medicine.symptom, 0210 nano-technology, Wound healing
Abstract: International audience; Despite the availability of different antibiotics, bacterial infections are still one of the leading causes of hospitalization and mortality. The clinical failure of antibiotic treatment is due to a general poor antibiotic penetration to bacterial infection sites as well as the development of antibiotic-resistant pathogens. In the case of skin infection, the wound is covered by exudate, making it impermeable to topical antibiotics. The development of a flexible patch allowing a rapid and highly efficient treatment of subcutaneous wound infections via photothermal irradiation is presented here. The skin patch combines the near-infrared photothermal properties of a gold nanohole array formed by self-assembly of colloidal structures on flexible polyimide films with that of reduced graphene oxide nanosheets for laser-gated pathogen inactivation. In vivo tests performed on mice with subcutaneous skin infection and treated with the photothermal skin patch show wound healing of the infected site, while nontreated areas result in necrotic muscular fibers and bacterial infiltrate. No loss in efficiency is observed upon multiple use of these patches during in vivo experiments because of their robustness.
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18. Expression of concern: Efficient capture and photothermal ablation of planktonic bacteria and biofilms using reduced graphene oxide-polyethyleneimine flexible nanoheaters.

Author: Budimir M, Jijie R, Ye R, Barras A, Melinte S, Silhanek A, Markovic Z, Szunerits S, and Boukherroub R
Abstract: Expression of concern for 'Efficient capture and photothermal ablation of planktonic bacteria and biofilms using reduced graphene oxide-polyethyleneimine flexible nanoheaters' by Milica Budimir et al. , J. Mater. Chem. B , 2019, 7 , 2771-2781, https://doi.org/10.1039/C8TB01676C.
Published: 2025
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19. Expression of concern: Near-infrared light activatable hydrogels for metformin delivery.

Author: Chengnan L, Pagneux Q, Voronova A, Barras A, Abderrahmani A, Plaisance V, Pawlowski V, Hennuyer N, Staels B, Rosselle L, Skandrani N, Li M, Boukherroub R, and Szunerits S
Abstract: Expression of concern for 'Near-infrared light activatable hydrogels for metformin delivery' by Li Chengnan et al. , Nanoscale , 2019, 11 , 15810-15820, https://doi.org/10.1039/C9NR02707F.
Published: 2024
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20. Expression of concern: Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters.

Author: Halouane F, Jijie R, Meziane D, Li C, Singh SK, Bouckaert J, Jurazek J, Kurungot S, Barras A, Li M, Boukherroub R, and Szunerits S
Abstract: Expression of concern for 'Selective isolation and eradication of E. coli associated with urinary tract infections using anti-fimbrial modified magnetic reduced graphene oxide nanoheaters' by Fatima Halouane et al. , J. Mater. Chem. B , 2017, 5 , 8133-8142, https://doi.org/10.1039/C7TB01890H.
Published: 2024
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21. Correction: Enterocin B3A-B3B produced by LAB collected from infant faeces: potential utilization in the food industry for Listeria monocytogenes biofilm management.

Author: Al-Seraih A, Belguesmia Y, Baah J, Szunerits S, Boukherroub R, and Drider D
Published: 2024
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22. Preparation of nanocellulose/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles for efficient catalytic reduction of 4-nitrophenol.

Author: Khili F and Omrani AD
Subjects: Catalysis, Nanocomposites chemistry, Aminophenols chemistry, Borohydrides chemistry, Photoelectron Spectroscopy, Nanoparticles chemistry, Oxides chemistry, Spectrum Analysis, Raman, Graphite chemistry, Nitrophenols chemistry, Copper chemistry, Cellulose chemistry, Oxidation-Reduction
Abstract: The paper reports on the preparation of cellulose nanocrystals/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles (CNC/rGO-Cu 2 O) through a simple solvothermal method and its application for 4-nitrophenol reduction to 4-aminophenol using sodium borohydride. The CNC/rGO-Cu 2 O nanocomposite was formed chemically by first mixing CNC and graphene oxide (GO) followed by complexation of the negatively charged functional groups of CNC/GO with Cu 2+ ions and subsequent heating at 100°C. This resulted in the simultaneous reduction of GO to rGO and the formation of Cu 2 O nanoparticles. The as-elaborated nanocomposite was firstly characterized using different techniques such as atomic force microscopy, scanning electron microscopy, transmission electron microscopy, UV-Vis spectrophotometry, Raman spectroscopy and x-ray photoelectron spectroscopy. Then, it was successfully applied for efficient catalytic reduction of 4-nitrophenol to 4-aminophenol using sodium borohydride: the reduction was completed in about 6 min. After eight times use, the catalyst still maintained good catalytic performance. Compared to CNC/rGO, rGO/Cu 2 O and free Cu 2 O nanoparticles, the CNC/rGO-Cu 2 O nanocomposite exhibits higher catalytic activity even at lower copper loading., (© 2024 Wiley Periodicals LLC.)
Published: 2024
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23. Editorial Expression of Concern: Visible light assisted hydrogen generation from complete decomposition of hydrous hydrazine using rhodium modified TiO 2 photocatalysts.

Author: Kumar P, Kumar A, Queffélec C, Gudat D, Wang Q, Jain SL, Boukherroub R, and Szunerits S
Published: 2024
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24. Corrigendum to "Entrapment of uropathogenic E. coli cells into ultra-thin sol-gel matrices on gold thin films: A low cost alternative for impedimetric bacteria sensing" [Biosens Bioelectron. 124-125 (2019) 161-166 DOI: 10.1016/j.bios.2018.10.029].

Author: Jafari H, Amiri M, Abdi E, Navid SL, Bouckaert J, Jijie R, Boukherroub R, and Szunerits S
Published: 2024
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25. Corrigendum to "Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins" [Biosens. Bioelectron. 146 (2019) 111736].

Author: Padra JT, Pagneux Q, Bouckaert J, Jijie R, Sundh H, Boukherroub R, Szunerits S, and Lindén SK
Published: 2024
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26. Modeling for nonlinear acoustic imaging of an isolated crack via standing waves in a 2D solid.

Author: Aleshin VV, Verma R, and Truyaert K
Abstract: This paper is concerned with numerical modeling for nondestructive imaging of defects in solids via standing waves excited by a periodic sinewave signal. The stationary solution, purely sinusoidal in an intact sample, contains higher harmonics when damage is present. These harmonics are generated by contact acoustic nonlinearity and form their own standing waves whose intensity maximum usually indicates the position of damage, in a way similar to resonant vibrometry experiments. The key point of the developed numerical tool that describes those wave phenomena is a model of planar damage (crack, delamination) considered here as an inner contact with rough surfaces and friction. The corresponding boundary conditions are given by the previously developed contact model based on the Method of Memory Diagrams (MMD) capable of automating the account for hysteretic frictional effects. Combination of the MMD for boundary conditions and a finite element formulation for waves in a volume (MMD-FEM model) provides a complete description which represents a numerical code applied here for nonlinear standing waves simulations. We present a number of examples obtained for idealized 2D geometry and reveal conditions in which both position and extent of damage are clearly seen as well as cases where only partial detection is possible., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
Published: 2023
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27. Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry.

Author: Hosu IS, Sobaszek M, Ficek M, Bogdanowicz R, and Coffinier Y
Subjects: Boron, Cytochromes c, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Reproducibility of Results, Lasers, Carbon chemistry, Ricin
Abstract: Detecting proteins via surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative and quantitative detection of Cytochrome C under optimized experimental conditions. Boron doped carbon nanowalls (B-CNWs) with a [B]/[C] ratio of 5000 ppm and growing time of 4 h have shown the best performance in terms of signal intensity and reliability. Then, the detection of ricin, a ribosomal-inhibiting protein (RIP) classified as category B bioterrorism agent by CDC (Centre of Disease and Control and Prevention), was performed. For the first time, direct SALDI-MS detection of ricin B chain was reported without tedious sample preparation steps or database interrogation, and results were obtained within few minutes and a limit of detection (LOD) of 0.5 pmol/μl was obtained. Thanks to the introduction of galactosamine residues on B-CNW, we were able to selectively detect ricin B chain protein in complex media such as serum and soft drinks with enhanced signal intensity. B-CNWs are not toxic and are adaptable to any commercial MALDI-TOF mass spectrometer, showing their great potential as SALDI based materials., Competing Interests: Declaration of competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
Published: 2023
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28. Construction of flexible fiber-shaped boron-doped diamond film and its supercapacitor application.

Author: Zhang J, Zhao Z, Zhang Z, Guo L, Xu L, Sun P, Wang M, Gao M, Li Y, Li D, and Boukherroub R
Subjects: Tantalum, Electrodes, Alkalies, Diamond, Boron
Abstract: Flexible fiber-shaped supercapacitors (FSSCs) are promising candidates as electrode materials for the development of deformable electronic devices. Although tremendous efforts have been focused on the preparation of flexible electrode materials, traditional FSSCs materials face problems of inferior stability and complicated processes. Boron-doped diamond (BDD) holds promise as a FSSC electrode, owing to its well-established preparation process, strong acid and alkali corrosion resistance, environmentally and skin-friendly characteristics. Here, we reported a novel strategy for the construction of BDD-based FSSCs by growing a BDD film on a flexible tantalum (Ta) fiber substrate through hot filament chemical vapor deposition technique. Results showed that the BDD fiber film featured 10 folds improvement in specific capacitance than a planar BDD electrode. A symmetric supercapacitor device was assembled using the BDD fiber electrode and achieved an energy density of 25.6 mJ cm -2 at a power density of 0.6 mW cm -2 , and a desirable stability with higher capacitance retention of 93.5% after 20,000 cycles. Furthermore, the symmetric BDD fiber device exhibited satisfactory bendability with high specific capacitance retention under various bending deformations. The findings in this research work hold promise for the fabrication of high performance flexible FSSCs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)
Published: 2023
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29. Double-side effect of B/C ratio on BDD electrode detection for heavy metal ion in water.

Author: Pei J, Yu X, Wei S, Boukherroub R, and Zhang Y
Abstract: BDD (Boron-doped Diamond) electrode may hold a promising application to detect heavy metal ions for actual water monitoring and early warning, but a poor understanding of influence mechanism of B/C ratio on detection performance is in the way of its fabrication and application. This work is intended to reveal the double-side effect of B/C ratio on detection performance of BDD electrode so as to facilitate its actual application. SBDD (Self-supported Boron-doped Diamond) electrode is introduced for the first time to get rid of the interference factors such as substrate. A systematic investigation is conducted for the influence of B/C ratio on microstructure and electrochemical behavior of SBDD electrodes. With the increase of B/C ratio, the grain size continuously increases, and the preferred orientation gradually changes from plane (220) to (111). The gradual increasing of impurity phase content indicates a deterioration of diamond phase quality. In addition, the electrode electrochemical behavior initially gets better then worse. SBDD electrode with a B/C ratio of 1/500 has the largest active surface area of 2.1 cm 2 , the smallest diffusion resistance and the highest signal response. Under optimal parameter set, the SBDD electrode enjoys a sensitivity of 0.42 μA L μg -1 cm -2 and a detection limit of 1.12 μg L -1 in a wide linear range of 5-120 ppb. The phase quality and grain morphology jointly contribute to the double-side effect. A suitable B-sp 3 -C content, preferred orientation of (111) and small particle size may make the performance improvement of BDD electrode available., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)
Published: 2021
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30. The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development.

Author: Tenenbaum M, Plaisance V, Boutry R, Pawlowski V, Jacovetti C, Sanchez-Parra C, Ezanno H, Bourry J, Beeler N, Pasquetti G, Gmyr V, Dalle S, Kerr-Conte J, Pattou F, Hirai SI, Regazzi R, Bonnefond A, Froguel P, and Abderrahmani A
Subjects: Animals, Cell Proliferation drug effects, Cyclin D1 genetics, Cyclin D1 metabolism, Cyclin D2 genetics, Cyclin D2 metabolism, Female, Glucose pharmacology, Humans, Insulin metabolism, Insulin-Secreting Cells cytology, MAP Kinase Kinase Kinases antagonists & inhibitors, MAP Kinase Kinase Kinases genetics, Mice, Mice, Inbred C57BL, Mitogen-Activated Protein Kinase 10 antagonists & inhibitors, Mitogen-Activated Protein Kinase 10 genetics, Obesity metabolism, Obesity pathology, Pancreas growth & development, Pancreas metabolism, RNA Interference, RNA, Small Interfering metabolism, Rats, Rats, Sprague-Dawley, Insulin-Secreting Cells metabolism, MAP Kinase Kinase Kinases metabolism, Mitogen-Activated Protein Kinase 10 metabolism, Signal Transduction drug effects
Abstract: Unveiling the key pathways underlying postnatal beta-cell proliferation can be instrumental to decipher the mechanisms of beta-cell mass plasticity to increased physiological demand of insulin during weight gain and pregnancy. Using transcriptome and global Serine Threonine Kinase activity (STK) analyses of islets from newborn (10 days old) and adult rats, we found that highly proliferative neonatal rat islet cells display a substantially elevated activity of the mitogen activated protein 3 kinase 12, also called dual leucine zipper-bearing kinase (Dlk). As a key upstream component of the c-Jun amino terminal kinase (Jnk) pathway, Dlk overexpression was associated with increased Jnk3 activity and was mainly localized in the beta-cell cytoplasm. We provide the evidence that Dlk associates with and activates Jnk3, and that this cascade stimulates the expression of Ccnd1 and Ccnd2, two essential cyclins controlling postnatal beta-cell replication. Silencing of Dlk or of Jnk3 in neonatal islet cells dramatically hampered primary beta-cell replication and the expression of the two cyclins. Moreover, the expression of Dlk, Jnk3, Ccnd1 and Ccnd2 was induced in high replicative islet beta cells from ob/ob mice during weight gain, and from pregnant female rats. In human islets from non-diabetic obese individuals, DLK expression was also cytoplasmic and the rise of the mRNA level was associated with an increase of JNK3, CCND1 and CCND2 mRNA levels, when compared to islets from lean and obese patients with diabetes. In conclusion, we find that activation of Jnk3 signalling by Dlk could be a key mechanism for adapting islet beta-cell mass during postnatal development and weight gain.
Published: 2021
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31. Local damage detection by nonlinear coda wave interferometry combined with time reversal.

Author: Smagin N, Trifonov A, Bou Matar O, and Aleshin VV
Abstract: Coda wave interferometry (CWI) is a sensitive ultrasound method for the detection of weak and local changes in complex inhomogeneous media. In a nonlinear modification of the method discussed here, a high-frequency probe coda is compared to its replica obtained in the presence of low-frequency pumping. If, after the filtering-out of low frequencies, the coda signals are different, this is attributed to nonlinear pump-probe interaction induced by contact acoustical nonlinearity in the damaged zone. Actually, the CWI methods are used for global inspection of complex media, such as for example, concrete structures. In this work, a step forward is made; it consists in combining the CWI with the time-reversal (TR) technique in order to allow one to focus the pump wave on a selected area in the structure and to detect and localize a flaw. Time-reverse pump is possible only in pulsed mode due to the spatio-temporal wave compression. By this reason, the particularities of coda wave mixing in conventionally used continuous and pulsed pump mode are considered. It has been experimentally observed that an aftereffect of a pulsed pump provides a nonlinear interaction between pump and probe waves of a sufficient overall level for defect detection with TR. Finally, it was shown that a TR focusing even with the minimal available quality i.e., with only one transducer produces a sufficient contrast allowing to distinguish intact and damaged zones with nonlinear CWI., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
Published: 2020
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32. Rate-dependent force-extension models for single-molecule force spectroscopy experiments.

Author: Benedito M, Manca F, Palla PL, and Giordano S
Subjects: Mechanical Phenomena, Microscopy, Atomic Force, Models, Chemical, Connectin chemistry, Filamins chemistry, Protein Folding, Single Molecule Imaging
Abstract: Single-molecule force spectroscopy techniques allow for the measurement of several static and dynamic features of macromolecules of biological origin. In particular, atomic force microscopy, used with a variable pulling rate, provides valuable information on the folding/unfolding dynamics of proteins. We propose here two different models able to describe the out-of-equilibrium statistical mechanics of a chain composed of bistable units. These latter represent the protein domains, which can be either folded or unfolded. Both models are based on the Langevin approach and their implementation allows for investigating the effect of the pulling rate and of the device intrinsic elasticity on the chain unfolding response. The theoretical results (both analytical and numerical) have been compared with experimental data concerning the unfolding of the titin and filamin proteins, eventually obtaining a good agreement over a large range of the pulling rates.
Published: 2020
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33. Heterologous Biosynthesis of Five New Class II Bacteriocins From Lactobacillus paracasei CNCM I-5369 With Antagonistic Activity Against Pathogenic Escherichia coli Strains.

Author: Belguesmia Y, Bendjeddou K, Kempf I, Boukherroub R, and Drider D
Abstract: Lactobacillus paracasei CNCM I-5369 isolated from a traditional Algerian dairy product produces extracellular inhibitory substances, namely, bacteriocins, which are active against a panel of pathogenic Escherichia coli strains. This activity was observed only at a narrow pH 4.5-5, and resulted to be heat stable and sensitive to the action of proteolytic enzymes, which indicate a proteinaceous nature. This new strain has a genome of 2,752,975 bp, with a 46.6% G + C ratio and contains at least 2664 coding sequences. The Bagel software analysis identified five open reading frames (ORFs) that are translated to new class II bacteriocin. Each ORF was cloned in frame with a His-tag tail and expressed in E. coli BL21 (DE3) (pLysS) strain. Of note, each fusion protein carrying any of these ORFs at the C- or N-terminal position resulted to be active against E. coli 184 strain used as target organism. This manuscript reports the first multi-bacteriocinogenic strain producing five new class II bacteriocins with activity against Gram-negative bacilli (GNB), namely, E. coli . Heterologous expression and activity of each new class II bacteriocin were demonstrated., (Copyright © 2020 Belguesmia, Bendjeddou, Kempf, Boukherroub and Drider.)
Published: 2020
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34. Tunable photo-responsive elastic metamaterials.

Author: Gliozzi AS, Miniaci M, Chiappone A, Bergamini A, Morin B, and Descrovi E
Abstract: The metamaterial paradigm has allowed an unprecedented space-time control of various physical fields, including elastic and acoustic waves. Despite the wide variety of metamaterial configurations proposed so far, most of the existing solutions display a frequency response that cannot be tuned, once the structures are fabricated. Few exceptions include systems controlled by electric or magnetic fields, temperature, radio waves and mechanical stimuli, which may often be unpractical for real-world implementations. To overcome this limitation, we introduce here a polymeric 3D-printed elastic metamaterial whose transmission spectrum can be deterministically tuned by a light field. We demonstrate the reversible doubling of the width of an existing frequency band gap upon selective laser illumination. This feature is exploited to provide an elastic-switch functionality with a one-minute lag time, over one hundred cycles. In perspective, light-responsive components can bring substantial improvements to active devices for elastic wave control, such as beam-splitters, switches and filters.
Published: 2020
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35. Purification for Carbon Nanotubes Synthesized by Flame Fragments Deposition via Hydrogen Peroxide and Acetone.

Author: Hammadi AH, Jasim AM, Abdulrazzak FH, Al-Sammarraie AMA, Cherifi Y, Boukherroub R, and Hussein FH
Abstract: Carbon nanotubes (CNTs) are synthesized by the flame fragment deposition (FFD) technique using Iraqi liquefied petroleum gas (LPG) as a source of carbon in a hand-made reactor at a low temperature (160 °C) without using a catalyst. Purification of the multi-walled carbon nanotubes (MWCNTs) is carried out using a two-step process consisting of sonication in 30 wt.% hydrogen peroxide (H 2 O 2 ) solution at room temperature to remove amorphous impurities adhering to the walls of the CNTs and carbon nanoparticles (CNPs), followed by sonication in an acetone bath to remove the polyaromatic hydrocarbons (PAH) formed during the LPG gas burning. Comprehensive characterizations such as X-ray diffraction (XRD), atomic force microscopy (AFM), thermo-gravimetric analysis (TGA), and transmission electron microscopy (TEM) were conducted to verify the efficiency of the purification process. The results clearly demonstrated that this process is promising for the purification of the synthesized CNTs.
Published: 2020
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36. Ultrasmall CuS-BSA-Cu 3 (PO 4 ) 2 nanozyme for highly efficient colorimetric sensing of H 2 O 2 and glucose in contact lens care solutions and human serum.

Author: Swaidan A, Addad A, Tahon JF, Barras A, Toufaily J, Hamieh T, Szunerits S, and Boukherroub R
Subjects: Animals, Cattle, Contact Lenses, Humans, Solutions, Colorimetry, Copper chemistry, Glucose analysis, Hydrogen Peroxide analysis, Phosphates chemistry, Serum Albumin, Bovine chemistry
Abstract: This work reports on the synthesis of organic-inorganic hybrid nanoscale materials, CuS-BSA-Cu 3 (PO 4 ) 2 . The developed nanoparticles were characterized by various techniques, such as X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), UV-vis absorption spectrophotometry, Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). The CuS-BSA-Cu 3 (PO 4 ) 2 were successfully applied as artificial colorimetric probes in sensing H 2 O 2 , the final outcome of glucose oxidation, and proved to be efficient peroxidase mimics for the catalytic conversion of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB), into a blue colored oxidized product (oxTMB) which can be easily visualized by the naked eye and monitored by a great absorption peak at 654 nm in the UV-vis spectrophotometry. A highly efficient, rapid, sensitive, and selective determination of H 2 O 2 and glucose have been achieved with very low detection limits of 22 nM, and 27.6 nM over 0-8 μM and 0-1000 μM linear ranges, respectively. Compared to CuS-BSA, CuS-BSA-Cu 3 (PO 4 ) 2 exhibited improved peroxidase-like catalytic activity. Based on these observations, the performance of this approach was successfully validated in contact lens care solutions and human serum samples., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
Published: 2020
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37. Highly efficient conversion of the nitroarenes to amines at the interface of a ternary hybrid containing silver nanoparticles doped reduced graphene oxide/ graphitic carbon nitride under visible light.

Author: Kumar A, Paul B, Boukherroub R, and Jain SL
Abstract: In this report, a ternary Ag-rGO/g-C 3 N 4 hybrid was synthesized by a simple hydrothermal approach for the photocatalytic reduction of nitroarene compounds into their corresponding amines under visible light. Importantly, the present method did not require reducing agents, like hydrazine hydrate; instead methanol has been used as a source of electrons and protons for the photoreduction process. After grafting of Ag NPs, a significant enhancement in the efficiency of the rGO/g-C 3 N 4 for the reduction of nitrobenzenes was observed. Under optimized experimental conditions, the conversion of nitrobenzene and yield of aniline were determined to be 99% and 98%, respectively under visible light illumination for 4 h. The nitrobenzene compounds bearing both electron donating and withdrawing groups were selectively converted into their corresponding aniline products without altering the functionality. The enhanced performance of the developed photocatalyst attributed to the effective separation of photoexcited electrons on the photocatalyst surface and their subsequent transfer for the reduction of nitrobenzene molecules., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier B.V. All rights reserved.)
Published: 2020
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38. Pumping effect of heterogeneous meniscus formed around spherical particle.

Author: Nakamura H, Ogawa T, Inoue M, Hori T, Mu L, Yoshikawa HN, Zoueshtiagh F, Dietze G, Tsukahara T, and Ueno I
Abstract: Hypothesis: A disturbance such as a microparticle on the pathway of a spreading droplet has shown the tremendous ability to accelerate locally the motion of the macroscopic contact line (Mu et al., 2017). Although this ability has been linked to the particle-liquid interaction, the physical mechanisms behind it are still poorly understood despite its academic interest and the scope of numerous industrial applications in need of fast wetting., Experiments: In order to better understand the mechanisms behind the particle-liquid interaction, we numerically investigate the pressure and velocity fields in the liquid film. The results are compared to experiments assessing the temporal shape variation of the liquid-film meniscus from which pressure difference around the particle is evaluated., Findings: The particle-induced acceleration of the film front depends both on the shape of the meniscus that forms around the particle foot and the liquid "reservoir" in the film that can be pumped thanks to the presence of the particle. The study validates the presence of three stages of pressure difference between the upstream and downstream regions of the meniscus around the particle, which leads to the local acceleration/deceleration of the macroscopic contact line. We indicate that asymmetric meniscus around the particle foot produces a net pressure force driving liquid and accelerating the liquid-film front., (Copyright © 2019 Elsevier Inc. All rights reserved.)
Published: 2020
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39. Interaction of cellulose and nitrodopamine coated superparamagnetic iron oxide nanoparticles with alpha-lactalbumin.

Author: Mohammadi F, Moeeni M, Li C, Boukherroub R, and Szunerits S
Abstract: In recent years, numerous studies have focused on the understanding of the interactions between proteins and nanoparticles (NPs). In this work, we focus on the interaction of bovine α-lactalbumin (BLA) with differently coated magnetic nanoparticles (MP): as formed MP, MPs stabilized with dopamine (MP dopamine ) and nanoparticles coated with cellulose (MP cellulose ). The influence of the coating on the nanoparticle-protein interaction is revealed by a set of different experiments. The binding affinity ( K A ) between BLA and these three structures was found to vary from 10 5 M -1 (for MPs) to 10 11 M -1 (MP cellulose ). The orientation of BLA and the involvement of amino acid residues in the process of interaction with magnetic nanoparticles were identified by molecular docking studies. In addition, circular dichroism spectra revealed that the conformation of BLA was conserved upon interaction with the magnetic nanoparticles., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)
Published: 2020
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40. Acoustic Sensing of Forces Driving Fast Capillary Flows.

Author: Bussonnière A, Antkowiak A, Ollivier F, Baudoin M, and Wunenburger R
Abstract: The popping sound of a bursting soap bubble is acquired using microphone arrays and analyzed using spherical harmonics decomposition. Using the theoretical framework of aeroacoustics, we demonstrate that this acoustic emission originates mainly from the capillary stresses exerted by the liquid soap film on the air and that it quantitatively reflects the out-of-equilibrium evolution of the flowing liquid film. This constitutes the proof of concept that the acoustic signature of violent events of physical or biological origin could be used to measure the forces at play during these events.
Published: 2020
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41. Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation.

Author: Liu J, Li C, Brans T, Harizaj A, Steene SV, De Beer T, De Smedt S, Szunerits S, Boukherroub R, Xiong R, and Braeckmans K
Subjects: Cell Membrane drug effects, Cell Membrane metabolism, Cell Membrane radiation effects, Cell Survival drug effects, Cell Survival radiation effects, Gene Transfer Techniques, HeLa Cells, Humans, Jurkat Cells, Lasers, Nanostructures chemistry, Transfection methods, Transfection statistics & numerical data, Graphite chemistry, Polyethylene Glycols pharmacology, Polyethyleneimine pharmacology, Quantum Dots
Abstract: Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporation efficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was >80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger macromolecules (FD70 and FD500) as well. Finally, we show that rGO is suitable for photoporation using a near-infrared laser to reach 80% FD10 positive HeLa cells at 80% cell viability. We conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency., Competing Interests: The authors declare no conflict of interest.
Published: 2020
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42. Influence of buried oxide layers of nanostructured SOI surfaces on matrix-free LDI-MS performances.

Author: Hamdi A, Hosu IS, and Coffinier Y
Subjects: Optical Phenomena, Surface Properties, Water chemistry, Lasers, Mass Spectrometry methods, Nanostructures chemistry, Oxides chemistry
Abstract: In this paper, we report on the nanostructuration of the silicon crystalline top layer of different "home-made" SOI substrates presenting various buried oxide (BOx) layer thicknesses. The nanostructuration was achieved via a one-step metal assisted chemical etching (MACE) procedure. The etched N-SOI substrate surfaces were then characterized by AFM, SEM and photoluminescence. To investigate their laser desorption/ionization mass spectrometry performances, the different surfaces have been assessed towards peptide mixtures. We have shown that the matrix-free LDI process occurred from surface heating after laser irradiation and was fostered by thermal confinement in the thin nanostructured Si surface layer. This thermal confinement was enhanced with the increase of the buried oxide layer thickness until an optimal thickness of 200 nm for which the best results in terms of signal intensities, peptide discrimination and spot to spot and surface to surface variations were found.
Published: 2020
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43. Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation.

Author: Liu J, Hebbrecht T, Brans T, Parthoens E, Lippens S, Li C, De Keersmaecker H, De Vos WH, De Smedt SC, Boukherroub R, Gettemans J, Xiong R, and Braeckmans K
Abstract: Fluorescence microscopy is the method of choice for studying intracellular dynamics. However, its success depends on the availability of specific and stable markers. A prominent example of markers that are rapidly gaining interest are nanobodies (Nbs, ~ 15 kDa), which can be functionalized with bright and photostable organic fluorophores. Due to their relatively small size and high specificity, Nbs offer great potential for high-quality long-term subcellular imaging, but suffer from the fact that they cannot spontaneously cross the plasma membrane of live cells. We have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their viability. Being a laser-based technology, it is readily compatible with light microscopy and the typical cell recipients used for that. Spurred by these promising initial results, we demonstrate here for the first time successful long-term imaging of specific subcellular structures with labeled nanobodies in living cells. We illustrate this using Nbs that target GFP/YFP-protein constructs accessible in the cytoplasm, actin-bundling protein Fascin, and the histone H2A/H2B heterodimers. With an efficiency of more than 80% labeled cells and minimal toxicity (~ 2%), photoporation proved to be an excellent intracellular delivery method for Nbs. Time-lapse microscopy revealed that cell division rate and migration remained unaffected, confirming excellent cell viability and functionality. We conclude that laser-induced photoporation labeled Nbs can be easily delivered into living cells, laying the foundation for further development of a broad range of Nbs with intracellular targets as a toolbox for long-term live-cell microscopy.
Published: 2020
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44. Panel measurement method for the determination of scattering coefficients and edge diffraction in an open water tank.

Author: Roux L, Pouille M, Audoly C, and Hladky AC
Abstract: Material effective properties of composite media or their acoustic performance are often required to be experimentally determined or verified. For underwater applications, systems can be characterized in an open water tank in which a conventional measurement configuration consists of the panel, an acoustic source, and two hydrophones, one on either side of the panel. However, panel measurements may be greatly complicated by waves diffracted by the panel edges. The three-point method is a technique that decomposes total pressure into contributions of the incident, reflected, transmitted, and edge-diffracted pressures. These contributions are determined using measurements at three positions. Reflection and transmission coefficients can then be obtained by removing the contribution of the edge-diffracted waves. The three-point method is applied to an aluminum reference panel for which the experimental scattering coefficients are in very good agreement with those of an infinite panel. The validity limits of the method are also studied. Finally, a pressure mapping shows that the three-point method leads to an accurate determination of the reflection and transmission coefficients for different positions despite strong variations of the edge-diffracted pressure.
Published: 2020
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45. High performance of 3D silicon nanowires array@CrN for electrochemical capacitors.

Author: Guerra A, Haye E, Achour A, Harnois M, Hadjersi T, Colomer JF, Pireaux JJ, Lucas S, and Boukherroub R
Abstract: Silicon nanowire (SiNW) arrays were coated with chromium nitride (CrN) for use as supercapacitor electrodes. The CrN layer of different thicknesses was deposited onto SiNWs using bipolar magnetron sputtering method. The areal capacitance of the SiNWs-CrN, as measured in 0.5 M H 2 SO 4 electrolyte, was as high as 180 mF cm -2 at a scan rate of 5 mV s -1 (equivalent to 31.8 mF cm -2 at 1.6 mA cm -2 ) with an excellent electrochemical retention of 92% over 15 000 cycles. This work paves the way toward using CrN modified 3D SiNWs arrays for micro-supercapacitor application.
Published: 2020
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46. Parametric interaction of gravity-capillary wave triads under radiation pressure of ultrasound.

Author: Krutyansky L, Preobrazhensky V, Makalkin D, Brysev A, and Pernod P
Abstract: Nonlinear parametric coupling of gravity-capillary waves (GCW) by ultrasound wave impinging on a liquid surface is studied experimentally. Modulation of the plane wave radiation pressure by dynamically curved surface of the liquid is considered as a mechanism of the coupling. The standing GCW modes are excited near antinodes by ultrasound beam of carrier frequency 1.030 MHz and coupled parametrically by a plane ultrasound wave with a frequency 1.035 MHz. The beam was modulated at resonance frequencies of GCW overtones f 2 =5.265 Hz and f 4 =7.873 Hz while intensity of the plane wave was modulated at frequency f p =2.657 Hz that corresponds to the nonlinear parametric resonance f p =2f 2 -f 4 . The amplitudes of the parametric interaction of GCW triads are measured and compared with critical condition for GCWs explosive instability., (Copyright © 2019 Elsevier B.V. All rights reserved.)
Published: 2020
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47. Mucin modified SPR interfaces for studying the effect of flow on pathogen binding to Atlantic salmon mucins.

Author: Padra JT, Pagneux Q, Bouckaert J, Jijie R, Sundh H, Boukherroub R, Szunerits S, and Lindén SK
Subjects: Animals, Bacterial Infections metabolism, Fish Diseases metabolism, Protein Binding, Salmo salar metabolism, Surface Plasmon Resonance methods, Bacterial Infections veterinary, Fish Diseases microbiology, Fish Proteins metabolism, Mucins metabolism, Salmo salar microbiology
Abstract: Knowledge on host-pathogen interactions contributes to the development of approaches to alleviate infectious disease. In this work, we developed a surface plasmon resonance (SPR) based method for investigating bacteria/mucins interactions. Furthermore, we investigated adhesion of three pathogens, Aeromonas salmonicida, Aeromonas hydrophila and Vibrio harveyi, to Atlantic salmon mucins isolated from different epithelial sites, using SPR and microtiter-based binding assays. We demonstrated that performing bacterial binding assays to mucins using SPR is feasible and has advantages over microtiter-based binding assays, especially under flow conditions. The fluid flow in the SPR is linear and continuous and SPR enables real-time reading of mucin-bacterial bonds, which provides an in vivo-like setup for analysis of bacterial binding to mucins. The variation between technical replicates was smaller using SPR detection compared to the adenosine 5'-triphosphate (ATP) bioluminescence assay in microtiter plates. Furthermore, we demonstrated that the effect of flow on pathogen-mucin interaction is significant and that bacterial adhesion differ non-linearly with flow rates and depend on the epithelial source of the mucin., (Copyright © 2019 Elsevier B.V. All rights reserved.)
Published: 2019
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48. Preparation of Low-Resistance and Residue-free ITO Films for Large-scale 3D Displays.

Author: Zhang Z, Yu X, Zhao W, Lu K, Ji X, and Boukherroub R
Abstract: The large-sized naked-eye three-dimensional (3D) display is a critical device in the real-time topographic survey for deep-sea scientific research. As a core component, the low-impedance transparent conductive indium tin oxide (ITO) thin-film electrode lacks a reliable industrial preparation method. In the 3D display, the grating element with a low-resistance ITO film electrode should have a good binocular parallax to drive the display favorably. However, an increase in the ITO film temperature during deposition may induce its crystallization, and its etching residue may cause a short circuit between the ITO electrodes and abnormal display operation. In this work, we propose a simple and straightforward technique to produce amorphous thin ITO films by controlling the water vapor flow rate during the deposition process. The obtained ITO amorphous thick film (300 nm) can be etched without leaving residues on the display surface, ensuring vivid display performance of the 3D display. A field test employing the 3D display, consisting of a 3D parallax barrier and a two-dimensional (2D) display, does not exhibit a short-circuit phenomenon caused by residues encountered in previous devices. This work makes the 3D display applicable for the real-time topographic survey on the basis of both satisfying the nonetching residue and the decrease of the resistance value.
Published: 2019
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49. Influence of the player on the dynamics of the electric guitar.

Author: Le Carrou JL, Paté A, and Chomette B
Abstract: The sound of the electric guitar is strongly dependent on the string vibration. Where a mode of the structure coincides with a mode of the fretted string, coupling between the string and structure occurs at that "deadspot." The coupling significantly lowers decay time, leading to the name [Paté, Le Carrou, and Fabre (2014). J. Acoust. Soc. Am. 135(5), 3045-3055]. But how the guitarist affects the dynamic behavior of the structure by grasping the neck, holding the instrument with the strap, or laying the instrument on his/her thigh remains to be investigated. This is the aim of the paper. Two methods are proposed to identify the modal parameters of the electric guitar structure, either by a classical modal analysis in simulated playing configuration, or by an operational modal analysis in real playing configuration. For this latter method, modal parameters are identified from dynamic measurements performed when each string is plucked. Both methods are compared and allow one to quantify the modal frequency modification and the added modal damping, which depend on the player's body-part in contact with the structure and on the modal shape considered. Consequences of these modal parameters on the modeled sound show that the player can increase the decay time close to a deadspot.
Published: 2019
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50. High spatial resolution electrochemical biosensing using reflected light microscopy.

Author: Munteanu RE, Ye R, Polonschii C, Ruff A, Gheorghiu M, Gheorghiu E, Boukherroub R, Schuhmann W, Melinte S, and Gáspár S
Abstract: If the analyte does not only change the electrochemical but also the optical properties of the electrode/solution interface, the spatial resolution of an electrochemical sensor can be substantially enhanced by combining the electrochemical sensor with optical microscopy. In order to demonstrate this, electrochemical biosensors for the detection of hydrogen peroxide and glucose were developed by drop casting enzyme and redox polymer mixtures onto planar, optically transparent electrodes. These biosensors generate current signals proportional to the analyte concentration via a reaction sequence which ultimately changes the oxidation state of the redox polymer. Images of the interface of these biosensors were acquired using bright field reflected light microscopy (BFRLM). Analysis showed that the intensity of these images is higher when the redox polymer is oxidized than when it is reduced. It also revealed that the time needed for the redox polymer to change oxidation state can be assayed optically and is dependent on the concentration of the analyte. By combining the biosensor for hydrogen peroxide detection with BFRLM, it was possible to determine hydrogen peroxide in concentrations as low as 12.5 µM with a spatial resolution of 12 µm × 12 µm, without the need for the fabrication of microelectrodes of these dimensions.
Published: 2019
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