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Gary Hamel y Michele Zanini presentan en esta obra un radical cambio de paradigma en la forma de gestionar las organizaciones, pasando de un enfoque donde predomina la burocracia a un término donde se enfatiza la maximización de la contribución humana acuñando el término “Humanocracia”.Los autores describen los grandes desafíos actuales que van desde una pandemia mundial, el cambio climático, la injusticia social, el desplazo del trabajo del hombre por la automatización, el estancamiento del crecimiento productivo, amenazas geopolíticas entre otras cosas, a su vez, abarcan en el mismo panorama oportunidades generadas como la inteligencia artificial, la 5g, el internet, la genómica, etc. En pocas palabras los grandes avances que supone la innovación.

International audience; This article was written with the intention of giving a brief recognition to all those women who with their courage, passion, talent and interest in the past helped to forge the current Maya archaeology. Participating as assistants or companions in explorations of discovery or as researchers in their own right, these women travelers, explorers and archaeologists have bequeathed to us a fundamental knowledge about the ancient cultures of Mexico since the beginning of the 20th century. In many ways, they have been decisive in the direction taken by professional research on the material remains of the peoples who inhabited Mesoamerica. In the Maya area and in the Yucatan in particular, several women took on the task of documenting and investigating the remains of the ancient Maya culture and made known to the world the majesty of its ruins, paintings and art. Ann Axtell Morris, Alice Dixon Le Plongeon, Adela Breton and Tatiana Proskouriakoff, among others, wandered among the ruins of ancient cities registering enigmatic signs and reproducing colorful scenes that showed the desires and concerns of a culture already forgotten by time. In this sense, this article serves as a modest but well-deserved tribute to those women who dedicated part of their lives to unraveling the mysteries of the Maya-culture. It is an attempt to reassess the contribution of these pioneering scholars and their contributions to the development of archaeological science today.; Este artículo fue escrito con la intención de hacer un breve reconocimiento a todas aquellas mujeres que con su coraje, pasión, talento e interés por el pasado ayudaron a forjar el estudio de la arqueología maya actual. Participando como ayudantes o acompañantes de exploraciones de descubrimiento, o como investigadoras en su propio derecho, estas mujeres viajeras, exploradoras y arqueólogas nos han legado un conocimiento fundamental sobre las antiguas culturas de México desde principios del siglo XX. En muchos sentidos, ellas han sido determinantes en el rumbo que ha tomado la investigación profesional sobre los vestigios materiales de los pueblos que habitaron Mesoamérica. En el área maya, y en Yucatán en particular, varias mujeres se dieron a la tarea de documentar e investigar los restos de la antigua cultura maya y dieron a conocer al mundo la majestuosidad de sus ruinas, pinturas y arte. Ann Axtell Morris, Alice Dixon Le Plongeon, Adela Breton y Tatiana Proskouriakoff, entre otras, deambularon entre las ruinas de antiguas ciudades registrando enigmáticos signos y reproduciendo coloridas escenas que evidenciaban los anhelos y preocupaciones de una cultura ya olvidada por el tiempo. En este sentido, sirva el presente artículo como un modesto pero merecido homenaje a aquellas mujeres que dedicaron parte de su vida a desentrañar los misterios de la cultura maya. En este trabajo intentamos revalorar la aportación de estas estudiosas pioneras y sus aportaciones al desarrollo de la ciencia arqueológica actual.; Cet article a été écrit en reconnaissance à toutes ces femmes qui, par leur courage, leur passion, leur talent et leur intérêt pour le passé, ont contribué à forger l’archéologie maya actuelle. En participant en tant qu’assistantes ou conjointes à des missions d’exploration ou en tant que chercheuses à part entière, ces femmes voyageuses, exploratrices et archéologues nous ont légué une connaissance fondamentale sur les anciennes cultures du Mexique depuis le début du XXe siècle. À bien des égards, elles ont joué un rôle décisif dans les orientations prises par la recherche professionnelle sur les vestiges matériels des peuples mésoaméricains. Dans la région maya, et au Yucatan en particulier, plusieurs femmes se sont chargées de documenter et d’enquêter sur les vestiges de l’ancienne culture maya et ont fait connaître au monde entier la majesté de ses ruines, ses peintures et son art. Ann Axtell Morris, Alice Dixon Le Plongeon, Adela Breton et Tatiana Proskouriakoff, entre autres, ont déambulé parmi les ruines d’anciennes villes pour enregistrer des signes énigmatiques et reproduire des scènes colorées qui dévoilaient les appétences et préoccupations d’une culture déjà oubliée par le temps. En ce sens, cet article constitue un hommage modeste mais bien mérité à ces femmes qui ont consacré une partie de leur vie à percer les mystères de la culture maya. Il s’agit d’une tentative de réévaluation de la contribution de ces pionnières et de leurs apports au développement de la science archéologique d’aujourd’hui.

International audience; Nutraceutical plants provide nutrients for the animal as well as secondary compounds that can affect the biology and survival of gastrointestinal nematodes (GIN). Current screening of nutraceutical plants is based on in vitro evidence of anthelmintic (AH) activity against different life stages of GIN, but nutritional information is omitted or scarce. This study proposes an integral in vitro screening protocol to identify the nutraceutical value of the foliage from plant species consumed by small ruminants, using Haemonchus contortus as a biological model. The leaves from Acacia collinsii, A. pennatula, Bunchosia swartziana, Gymnopodium floribundum, Havardia albicans, Leucaena leucocephala, Lysiloma latisiliquum, Mimosa bahamensis, Piscidia piscipula, and Senegalia gaumeri were evaluated for their chemical composition and in vitro digestibility. Acetone:water extracts (70:30) from leaves of each plant were evaluated using the egg hatch assay and larval exsheathment inhibition assay. Respective effective concentrations 50% (EC50) were determined for each assay. The ten plant species showed good nutritional value for ruminants, including crude protein (> 10%), metabolizable energy (> 2.9 MJ/kg DM), and varied CT content (from 1.0 to 37.6%). The best AH activity against H. contortus eggs (EC50 = 401.8 μg/mL) and L3 (EC50 = 83.1 μg/mL) was observed for S. gaumeri extract. Although all the plant species showed in vitro nutraceutical potential, the leaves of S. gaumeri had the best values. The proposed in vitro protocol showed to be useful for the integral assessment of the nutraceutical potential of different plant species as it included the nutritional value and the AH activity against eggs and L3 in the selected plant species.

International audience; This study performed a Life Cycle Assessment (LCA) to evaluate the environmental impact of chicken meat production from a Mexican case study, with a “cradle-to-slaughterhouse gate” approach. To overcome the LCA's limitations and provide a more holistic picture of the system, simulation and artificial intelligence techniques were integrated. First, raw material/energy requirements were obtained from the case study and simulated using Process simulation (PS) and Monte Carlo (MC) simulation to estimate the emissions and quantify their uncertainty. Then, IMPACT 2002 + was used to calculate the overall impact using Ecoinvent and LCA Food databases. The results highlight that chicken farms are the main factors responsible for the environmental impacts assessed, where feed production (use of chemicals and energy requirements) and on-farm emissions (organic waste decomposition) are the main contributors. Concerning the slaughterhouse, the energy production (electricity and steam) and the cooling-related activities present a significant impact. Afterwards, three impact allocation procedures (mass method, neural networks, and stepwise regression) were tested, showing similar results. Finally, a multiobjective optimization model based on a Genetic Algorithm was applied looking to minimize the environmental impacts and maximize the economic benefits. The selected alternative achieved a reduction of 15.14% per functional unit at the environmental indicators. The results encourage the use of support techniques for LCA to perform a reliable assessment and an environmental/economic optimization of the system.

This study assessed the ultrastructural changes caused in adult Haemonchus contortus obtained from goats fed fodder based on polyphenol-rich plants Lysiloma latisiliquum or Onobrychis viciifolia or from goats drenched with quebracho extract, Schinopsis spp. The H. contortus were obtained from artificially infected goats used as models to investigate the anthelmintic effect of feeding or drenching with the polyphenol-rich materials. Nematode populations were exposed to polyphenol-rich plant materials by feeding host goats for 8 consecutive days (D28 to D35 post-infection) with (a) L. latisiliquum fodder at 800 g fresh basis/day, (b) O. viciifolia fodder offered ad libitum, and (c) drenched with a solution containing quebracho extract (90 g/day). Meanwhile, control H. contortus were obtained from goats fed polyphenol-free diets. The H. contortus specimens were recovered from the goats on D36 post-infection, and transmission electron microscopy (TEM) was used to identify ultrastructural changes. In vivo exposure to different polyphenol-rich plant materials caused vacuolization of the nematodes' intestinal, muscular and hypodermal cells. These alterations represent the first evidence of cell damage caused in H. contortus when hosts were fed or drenched with polyphenol-rich materials. Ultrastructural changes affecting several types of cells could explain modifications in worm motility and nutrition, eventually affecting H. contortus reproductive success. This study contributes to our understanding of the mechanisms of action of polyphenol-rich plants against H. contortus when given as nutraceuticals to goats.Étude ultrastructurale d’Haemonchus contortus adultes exposés in vivo à des matériaux riches en polyphénols chez les chèvres.Cette étude a examiné les lésions ultrastructurales provoquées chez les adultes d’Haemonchus contortus obtenus chez des chèvres alimentées par des fourrages riches en polyphénols, Lysiloma latisiliquum ou Onobrychis viciifolia, ou des chèvres recevant des extraits de quebracho, Schinopsis spp. Les H. contortus ont été obtenus à partir de chèvres artificiellement infestées pour vérifier les effets anthelminthiques de l’alimentation ou l’administration de ressources riches en polyphénols. Les populations de nématodes ont été exposées à des ressources végétales contenant des polyphénols chez des hôtes infestés recevant pendant 8 jours (J28 à J35 après infestation) : (a) un fourrage à base de L. latisiliquum (800 g/jour), (b) un fourrage à base de sainfoin (O. viciifolia) offert ad libitum, et (c) une solution d’extrait de quebracho (90 g/jour). Pendant ce temps, une population témoin d’H. contortus a été obtenue à partir de chèvres alimentées avec un régime à base de fourrages sans polyphénols. Des échantillons d’H. contortus ont été obtenus à partir des divers groupes de chèvres à J36 après infestation, et la microscopie électronique à transmission a été utilisée pour identifier les modifications ultrastructurales. L’exposition in vivo à différents matériaux végétaux riches en polyphénols chez l’hôte a provoqué chez les nématodes une vacuolisation des cellules intestinales, musculaires et hypodermiques. Ces altérations représentent la première preuve de lésions cellulaire provoquées chez H. contortus lorsque l’hôte reçoit des ressources riches en polyphénols. Les changements ultrastructuraux affectant divers types de cellules pourraient expliquer des modifications fonctionnelles de motilité et de nutrition des vers, et finalement modifier le succès reproducteur d’H. contortus. Cette étude contribue à la compréhension des mécanismes d’action des plantes contenant des polyphénols utilisées comme alicament contre H. contortus.

International audience; Small ruminants browsing in tropical forests readily consume the foliage of Senegalia gaumeri. A S. gaumeri methanol:water extract was recently shown to have ovicidal activity against Haemonchus contortus eggs in vitro. In the present study, the fraction of a S. gaumeri methanol:water extract with ovicidal activity against H. contortus eggs and the metabolites potentially involved in this activity were identified. Bio-guided fractionation of the S. gaumeri methanol:water extract identified high ovicidal activity (80.29%, EC50 = 58.9 μg/mL) in the non-polar sub-fraction P1. Gas chromatography-mass spectrometry (GC–MS) identified several fatty acids: pentacosane (18.05%), heneicosane (18.05%), triacontane (30.94%), octacosane (18.05%), and hexanedioic acid bis-(2-ethylhexyl) ester (32.72%). Purification of the polar components of sub-fraction P1 led to the identification of p-coumaric acid as a major constituent. In egg hatch tests, 400 μg/mL p-coumaric acid resulted in an ovicidal effect of 8.7%, a larvae failing eclosion effect of 2.9%, and of the emerged larvae (88.4%), many were damaged. In conclusion, the low AH activity of p-coumaric acid against H. contortus eggs indicates that it is not solely responsible for the ovicidal activity of sub-fraction P1 but might act in synergy with other compounds in this fraction. However, p-coumaric acid showed potential anthelmintic effects against the larval stage of H. contortus.

International audience; Because of the central position of the chemical industries along the value chain, process design has a pivotal role, involving many decision makers and multiple levels of decisions. To tackle the environmental concern at source, this article presents a methodological framework for process ecodesign, coupling flowsheeting simulators both for production and energy processes with a Life Cycle Assessment module that generalizes and automates the evaluation of environmental impacts. The life cycle inventory is carried out through the combined use of mass and energy balances resulting both from the global simulation of the process and its associated energy production requirement and from the use of inventory database (i.e., Ecoinvent v3) embedded in the Life Cycle Assessment software tool used (SimaPro). Different process alternatives can thus be evaluated in a systematic way and the energy‐related emissions for any given process that match exactly the real situation can be computed without introducing a bias in the estimation. Through comparisons between a case base and process alternatives, a systematic decision can be made in terms of whether a solution is moving the process towards a more sustainable operation. The effectiveness of the proposed framework is first illustrated through the case study of benzene production and second, by a biodiesel production process from waste vegetable oils which is one of the foremost alternative fuels to those refined from petroleum products

International audience; In vitro studies using plant extracts suggest a relationship between their polyphenol contents and their anthelmintic (AH) activity against Haemonchus contortus. High polyphenol content appears to increase the efficacy of plant extracts against H. contortus as assessed by the larval exsheathment inhibition assay (LEIA) while appearing to reduce the AH efficacy measured using the egg hatch assay (EHA). In addition, some plants lack AH activity. Therefore, the present study investigated the relationship between the contents of condensed tannins (CT), total phenols (TP), and total tannins (TT) in methanol:water extracts (70:30) obtained from ten tropical plant species consumed by small ruminants as well as their AH activity against H. contortus evaluated by LEIA and EHA. Extracts of Acacia collinsii, Lysiloma latisiliquum, Havardia albicans, Senegalia gaumeri, Mimosa bahamensis, Piscidia piscipula, Acacia pennatula, Gymnopodium floribundum, Leucaena leucocephala, and Bunchosia swartziana were examined. Positive correlations were found between the effective concentration 50% (EC50) (EHA) of extracts and their CT (r = 0.6809, P < 0.05, n = 10) and TP (r = 0.9152, P < 0.05, n = 10) content, suggesting that their concentration negatively affected AH activity against eggs. Based on the LEIA, there was no significant association between the EC50 and the CT, TP, or TT of all extracts evaluated. Thus, if sheep and goats consume a complex feed mixture with high amounts of CT, TP, and TT, it might be difficult to observe an AH effect against H. contortus egg hatching. However, the AH effect upon L3 establishment might be feasible.

International audience; This study explored the variation in susceptibility to acetone:water plant extracts between infective larvae (L3) of ten Haemonchus contortus isolates from different geographical origin. The L3 of 10 different isolates were exposed either to the acetone:water extract of a temperate plant (Onobrychis viciifolia) or a tropical plant (Acacia pennatula) and were evaluated with the larval exsheathment inhibition assay (LEIA). The L3 of each isolate were incubated with different concentrations of each extract (0, 25, 50, 100, 200, 400, 600, 800, 1000 and 1200μg/mL of phosphate buffered saline (PBS)). After incubation, the exsheathment process of L3 was induced using a solution with sodium hypochlorite (2%) and sodium chloride (16.5%). The proportion of exsheathed L3 was determined for each concentration at 0, 20, 40 and 60min. Effective concentrations 50% (EC50) and the corresponding 95% confidence intervals (95% CI) were calculated for every isolate with both extracts. Moreover, a resistance ratio (RR) was calculated for each extract to compare isolates, using the most susceptible isolate as the respective reference for each extract. To determine the role of polyphenols on the reported effect, a second set of incubations was made for each isolate and each extract, using the extracts at a concentration of 1200μg/mL PBS with or without polyvinylpolypyrrolidone (PVPP), a polyphenol blocking agent, and controls without extract. The ten different H. contortus isolates showed variation in susceptibility for each of the 2 extracts tested (P

International audience; The aim of the study was to examine the variation in the in vitro susceptibility of ten Haemonchus contortus isolates from different geographical origins using respective egg hatch assays (EHA) with acetone:water extracts of two tannin containing plants, chimay (Acacia pennatula) and sainfoin (Onobrychis viciifolia). Fresh eggs were incubated in PBS with different concentrations of each extract (0, 600, 1200, 2400, 3600, 5000 and 8000μg/ml PBS). Additional concentrations were tested for O. viciifolia (75, 100, 200 and 400μg/ml PBS). Effective concentrations 50% (EC50), with the corresponding 95% confidence interval (95% CI), were calculated for every isolate with both extracts. Moreover, a resistance ratio (RR) was calculated to compare the isolates, using the most susceptible isolate for each extract as the respective reference. A second set of incubations were made using polyvinylpolypyrrolidone (PVPP) (0, 5000μg/ml, 5000μg/ml+PVPP) to determine the influence of polyphenols on the AH effect. The proportion of morulated eggs, eggs with L1 larvae failing eclosion (%LFE), and emerged larvae were estimated at different extract concentrations. Data of each isolate was used to calculate the effective concentration 50% (EC50) for each extract. The EC50 of each isolate was used to determine resistance ratio (RR) for the different isolates. For the 2 extracts, a susceptibility variation in egg hatching was observed for the different H. contortus isolates. The EC50 values for A. pennatula ranged from 2203 to 14106μg (RR from 2.01 to 6.40). The O. viciifolia extract showed higher variability with EC50 values ranging from 104 to 4783μg (RR from 3.66 to 45.74). The main AH effects of the two extracts tested on the ten isolates consisted in blocking the emergence of L1 larvae (higher% LFE). Additional observations on emerged larvae showed that extract exposure caused alterations in the internal structure, separating the cuticle from the pharynx, bulb and intestinal cells. The use of PVPP revealed that (a) condensed tannins were not the sole plant secondary metabolites responsible for the AH effects, and (b) different H. contortus isolates showed variability in the role of tannins either on the ovicidal effect or the %LFE.

Fil: Galetto, Leonardo. Universidad Nacional de Córdoba. Córdoba, Argentina. Fil: Aizen, Marcelo A. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente. Rio Negro, Argentina. Fil: Arizmendi, María del Coro. Universidad Nacional Autónoma de México. México. Fil: Freitas, Breno M. Universidade Federal do Ceará. Brasil. Fil: Garibaldi, Lucas A. Universidad Nacional de Río Negro. Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural. Río Negro, Argentina. Fil: Garibaldi, Lucas A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural. Río Negro, Argentina. Fil: Giannini, Tereza C. Instituto Tecnológico Vale Desenvolvimento Sustentavel. Brasil. Fil: Lopes, Ariadna V. Universidade Federal de Pernambuco. Brasil. Fil: Do Espírito Santo, Mário M. Universidade Estadual de Montes Claros. Brasil. Fil: Maués, Márcia M. Embrapa Amazônia Oriental. Brasil. Fil: Nates-Parra, Guiomar. Universidad Nacional de Colombia. Colombia. Fil: Rodríguez, Jaime I. Museo Nacional de Historia Natural de Bolivia. Bolivia. Fil: Quezada-Euán, José J. G. Universidad Autónoma de Yucatán. México. Fil: Vandame, Remy. El Colegio de la Frontera Sur. México. Fil: Viana, Blandina F. Universidade Federal da Bahia. Brasil. Fil: Imperatriz-Fonseca, Vera L. Instituto Tecnológico Vale Desenvolvimento Sustentavel. Brasil. The conservation status of pollinators and pollination in Latin America (LA) is reviewed. The knowledge regarding native and managed pollinators (e.g., honeybee and stingless bees) and pollination services was synthetized, and the guidelines to improve the opportunities for conservation are provided, considering the threats to pollinators and the perspectives from traditional and local knowledge. The analysis indicates that diverse threats (e.g., large-scale agriculture, deforestation, overuse of agrochemicals) are linked with pollination and pollinator decline, which affect the reproduction of most native plants and the yields of many crops. LA harbours the highest bee diversity worldwide, with 26% of the total recorded species, and it is a biodiversity hotspot of vertebrate pollinators, including hummingbirds, perching birds, nectarivorous bats and other mammal pollinators. Specific recommendations to conserve native pollinators and to improve pollination services are provided, which could be considered by stakeholders and governments aiming to elaborate biocultural conservation. For example, introducing policies and legal responses for incentives to help farmers maintain natural habitats and forests, to replace or reduce agrochemicals and to improve diversified crop production with agroecological practices; refining agrochemical regulations to minimize the exposure of pollinators to insecticides and herbicides; improving knowledge and education on pollinators and pollination gives societies worldwide the opportunity to change current hegemonic agricultural practices and consumption paerns; integrating different land ethical views of ethnic minorities on a sustainable relationship between production and biodiversity. A wider view combining social, ecological, cultural dimensions may support beer decision making. This holistic socio-agroecological perspective is urgently needed to conserve and manage pollinators at different spatial and temporal scales, and to integrate pollination services, pollinator-friendly habitat management approaches and diversified farming systems. Se realizó una revisión sobre el estado de conservación de los polinizadores y la polinización en América Latina (LA). Se presentan pautas para mejorar las oportunidades de conservación, considerando las amenazas a los polinizadores y las perspectivas desde el conocimiento tradicional y local. El análisis indica que diversas amenazas (e.g., agricultura a gran escala, deforestación, uso excesivo de agroquímicos) están vinculadas con la disminución de polinizadores, afectando la reproducción de las plantas nativas y los rendimientos de muchos cultivos. LA alberga la mayor diversidad de abejas en todo el mundo y una gran diversidad de polinizadores vertebrados (e.g., colibríes, aves de percha nectarívoras, murciélagos nectarívoros y otros mamíferos). Se proporcionan recomendaciones para proteger los polinizadores nativos y mejorar los servicios de polinización, las que podrían ser consideradas por los tomadores de decisiones y así promover la conservación biocultural. Por ejemplo, desarrollar instrumentos legales, políticas e incentivos para ayudar a los agricultores a mantener los hábitats naturales, para reemplazar o reducir el uso de agroquímicos y para promover las prácticas agroecológicas; perfeccionar las reglamentaciones sobre aplicación de agroquímicos para minimizar la exposición de los polinizadores a insecticidas y herbicidas; mejorar la comunicación pública del conocimiento sobre los polinizadores y la polinización para incentivar un cambio en las prácticas agrícolas hegemónicas y los patrones de consumo actuales; considerar otras éticas ambientales de las minorías étnicas para enfatizar la necesidad de promover una relación sostenible entre producción de alimentos y biodiversidad. Se necesita urgentemente una visión más amplia que combine las dimensiones sociales, ecológicas y culturales para una mejor toma de decisiones. Esta perspectiva socio-agroecológica holística es importante para conservar y gestionar los polinizadores a diferentes escalas espaciales y temporales, y para poder integrar los servicios de polinización con enfoques de gestión del territorio favorables a los polinizadores y con sistemas agrícolas diversificados.

Reliable diagnostic tools are needed to choose the appropriate treatment and proper control measures for animal trypanosomoses, some of which are pathogenic.Trypanosoma cruzi, for example, is responsible for Chagas disease in Latin America. Similarly, pathogenic animal trypanosomoses of African origin (ATAO), including a variety ofTrypanosomaspecies and subspecies, are currently found in Africa, Latin America and Asia. ATAO limit global livestock productivity and impact food security and the welfare of domestic animals. This review focusses on implementing previously reviewed diagnostic methods, in a complex epizootiological scenario, by critically assessing diagnostic results at the individual or herd level. In most cases, a single diagnostic method applied at a given time does not unequivocally identify the various parasitological and disease statuses of a host. These include “non-infected”, “asymptomatic carrier”, “sick infected”, “cured/not cured” and/or “multi-infected”. The diversity of hosts affected by these animal trypanosomoses and their vectors (or other routes of transmission) is such that integrative, diachronic approaches are needed that combine: (i) parasite detection, (ii) DNA, RNA or antigen detection and (iii) antibody detection, along with epizootiological information. The specificity of antibody detection tests is restricted to the genus or subgenus due to cross-reactivity with otherTrypanosomaspp. and Trypanosomatidae, but sensitivity is high. The DNA-based methods implemented over the last three decades have yielded higher specificity and sensitivity for active infection detection in hosts and vectors. However, no single diagnostic method can detect all active infections and/or trypanosome species or subspecies. The proposed integrative approach will improve the prevention, surveillance and monitoring of animal trypanosomoses with the available diagnostic tools. However, further developments are required to address specific gaps in diagnostic methods and the sustainable control or elimination of these diseases.Graphical Abstract

International audience; Most neglected tropical diseases (NTDs) are intrinsically embedded within the One Health approach: NTD researchers have already been dealing with multidisciplinary and intersectoral work for decades simply because it is essential for understanding and controlling the usually complex transmission of the pathogens causing NTDs.This long experience has already enrooted the idea of the horizontal integration of research, control, elimination and eradication strategies. The ongoing epidemiological transitions of most NTDs urges pursuing and amplifying the development of co-constructed multidisciplinary and intersectoral research initiatives for improving control/elimination/eradication processes. Lessons from NTDs may also be useful for other diseases targeted by ongoing One Health initiatives.

International audience; Premise : Abiotic factors and plant species traits have been shown to drive latitudinal gradients in herbivory, and yet, population‐level factors have been largely overlooked within this context. One such factor is plant density, which may influence the strength of herbivory and may vary with latitude.Methods : We measured insect herbivory and conspecific plant density (CPD) of oak (Quercus robur) seedlings and saplings along a 17° latitudinal gradient (2700 km) to test whether herbivory exhibited a latitudinal gradient, whether herbivory was associated with CPD, and whether such an association changed with latitude.Results : We found a positive but saturating association between latitude and leaf herbivory. Furthermore, we found no significant relationship between CPD and herbivory, and such lack of density effects remained consistent throughout the sampled latitudinal gradient.Conclusions : Despite the apparently negligible influence of plant density on herbivory for Q. robur, further research with other plant taxa and in different types of plant communities are needed to investigate density‐dependent processes shaping geographical variation in plant–herbivore interactions.

The world is rapidly urbanizing, thereby transforming natural landscapes and changing the abundance and distribution of organisms. However, insights into the effects of urbanization on species interactions, and plant–pathogen interactions in particular, are lacking. We investigated the effects of urbanization on powdery mildew infection on Quercus robur at continental and within-city scales. At the continental scale, we compared infection levels between urban and rural areas of different-sized cities in Europe, and investigated whether plant traits, climatic variables and CO emissions mediated the effect of urbanization on infection levels. Within one large city (Stockholm, Sweden), we further explored whether local habitat features and spatial connectivity influenced infection levels during multiple years. At the continental scale, infection severity was consistently higher on trees in urban than rural areas, with some indication that temperature mediated this effect. Within Stockholm city, temperature had no effect, while local accumulation of leaf litter negatively affected powdery mildew incidence in one out of three years, and more connected trees had lower infection levels. This study is the first to describe the effects of urbanization on plant–pathogen interactions both within and among cities, and to uncover the potential mechanisms behind the observed patterns at each scale., This research was supported by a grant from the Swedish Research Council Vetenskapsrådet (2015-03993 to AJMT)

Aim: It is predicted that insular plant taxa have evolved reduced defences in response to lower herbivore pressure on islands. However, the few studies testing this hypothesis have addressed variation in individual defensive traits, without paying attention to patterns of correlated trait expression (i.e. defence syndromes). Location: Balearic and Canary Islands. Taxon: Ninety-one woody plant species. Methods: We tested whether plant species with contrasting histories of insularity (namely, endemics, non-endemic natives and exotics) differed in their defensive syndromes using a suite of morphological, physical, and chemical traits putatively associated with herbivory. For this, we measured eight leaf traits of 42 endemic, 29 native non-endemic and 20 introduced species for which specimens were sourced from botanical gardens found in two archipelagos: the Balearic and Canary Islands. Results: We conducted phylogenetic-controlled analyses which showed that, contrary to predictions, insular taxa (endemics and non-endemic natives) across both archipelagos were more defended (thicker, smaller leaves with less nutrients) than exotic species. There were no differences in chemical defence (phenolic compounds) between endemics, non-endemic natives and exotics. Finally, we also found different defence syndromes between archipelagos: whereas species from the Balearic Islands were more physically defended, on average, those from the Canary Islands had higher chemical defences. Main conclusions: Overall, these results point to a defence syndrome based on low-nutrient and physically defended leaves characteristic of insular plant taxa that is indistinct for endemic and non-endemic taxa, relative to introduced species, as well as quantitative and qualitative differences in defences between archipelagos owing to changes in species composition and likely also to different histories of biotic or abiotic pressure., This research was financially supported by a grant from the Spanish Ministry of Science, Innovation and Universities (RTI2018-099322-B- I00) and the Ramón y Cajal Research Programme (RYC-2013- 13230) to XM. CG-V was supported by a Vicenç Mut Grant (Govern de les Illes Balears, Conselleria d'Innovació, Recerca i Turisme and the European Social Fund). No permits were required for this research.

[Aim] Plants on islands are often subjected to lower levels of herbivory relative to those found at mainland sites. As a consequence, island plants are predicted to exhibit lower levels of physical or chemical defences, which renders them more susceptible to introduced herbivores. Yet, instances of high pressure by superabundant herbivores native to islands have been reported in many insular systems, which presumably would result in heightened plant defences. To date, no quantitative review has been conducted to determine how common these contrasting patterns are and their implications for the evolution of plant-herbivore interactions., [Location] Islands worldwide., [Taxon] Plants, insects, molluscs, mammals., [Methods] We conducted a meta-analysis of insularity effects on herbivory and plant defences by including studies that involved island-mainland comparisons of the same plant species in both environments (90% of cases), or insular endemics versus mainland congeners (10% of cases). We tested for differences between mammalian and invertebrate (mollusc or insect) herbivory as well as between plant chemical and physical defences by specifying comparisons based on the type of herbivore (vertebrate or invertebrate) or plant trait included in the study., [Results] Mammalian herbivory was significantly higher on islands than on mainlands. In contrast, no significant effect was observed on invertebrate herbivory. In addition, we found no significant difference in either plant physical or chemical defences between insular and mainland plants, though physical defences tended to be higher for plants on islands., [Main conclusions] All analysed mammal studies focused on species introduced to islands, suggesting greater susceptibility of insular plants to exotic mammals, whereas the lack of effects in the case of invertebrate herbivory suggests no difference in susceptibility to molluscs or insects between insular and mainland plants. Interestingly, plant trait patterns suggest a trend for increased physical defences by insular plants, possibly due to heightened pressure by exotic mammalian herbivores on islands, whereas chemical defences appear uncorrelated to differences in herbivory. These findings call for further experimental and observational studies measuring defences and herbivory for multiple sympatric plant species occurring at both mainland and island sites within a system, or comparing insular endemics to congeneric mainland species., This research was financially supported by a grant from the Regional Government of Galicia (IN607D 2016/001), a grant from the Spanish Ministry of Science, Innovation and Universities (RTI2018-099322-B-100) and the Ramón y Cajal Research Programme (RYC-2013-13230) to XM. CG-V is supported by a postdoctoral Vicenç Mut fellowship (Govern de les Illes Balears, Conselleria d'Innovació, Recerca i Turisme and the European Social Fund).

Background and AimsClassic theory on geographical gradients in plant–herbivore interactions assumes that herbivore pressure and plant defences increase towards warmer and more stable climates found at lower latitudes. However, the generality of these expectations has been recently called into question by conflicting empirical evidence. One possible explanation for this ambiguity is that most studies have reported on patterns of either herbivory or plant defences whereas few have measured both, thus preventing a full understanding of the implications of observed patterns for plant–herbivore interactions. In addition, studies have typically not measured climatic factors affecting plant–herbivore interactions, despite their expected influence on plant and herbivore traits.MethodsHere we tested for latitudinal variation in insect seed predation and seed traits putatively associated with insect attack across 36 Quercus robur populations distributed along a 20° latitudinal gradient. We then further investigated the associations between climatic factors, seed traits and seed predation to test for climate-based mechanisms of latitudinal variation in seed predation.Key ResultsWe found strong but contrasting latitudinal clines in seed predation and seed traits, whereby seed predation increased whereas seed phenolics and phosphorus decreased towards lower latitudes. We also found a strong direct association between temperature and seed predation, with the latter increasing towards warmer climates. In addition, temperature was negatively associated with seed traits, with populations at warmer sites having lower levels of total phenolics and phosphorus. In turn, these negative associations between temperature and seed traits led to a positive indirect association between temperature and seed predation.ConclusionsThese results help unravel how plant–herbivore interactions play out along latitudinal gradients and expose the role of climate in driving these outcomes through its dual effects on plant defences and herbivores. Accordingly, this emphasizes the need to account for abiotic variation while testing concurrently for latitudinal variation in plant traits and herbivore pressure.

This paper aims to describe the construction and operation of a respiration chamber of the head-box type for methane (CH4) measurements in bovines. The system consists of (1) a head box with a stainless steel frame and acrylic walls, floor, and ceiling, (2) a stainless steel feeder, (3) an automatic drinking water bowl, (4) a hood made from reinforced canvas, (5) an infrared (IR) CH4 gas analyzer, a mass flow generator, a data-acquisition system, and (6) a steel metabolic box. Six assays were conducted to determine the pure CH4 recovery rate of the whole system in order to validate it and comply with standards of chamber operation. The gravimetrical method was used for the recovery test and the recovery rate obtained was 1.04 &plusmn, 0.05. Once the system was calibrated, measurements of CH4 were conducted using eight animals consisting of four Holstein cows with a live weight of 593.8 &plusmn, 51 kg and an average milk yield of 23.3 &plusmn, 1.8 kg d&minus, 1 and four heifers with a live weight of 339 &plusmn, 28 kg. The CH4 production values were 687 &plusmn, 123 and 248 &plusmn, 40 L CH4 d&minus, 1 for cows and heifers, respectively. The CH4 yield was 19.7 &plusmn, 3.4 g and 17.1 &plusmn, 3.4 g CH4 kg&minus, 1 of dry matter consumed for cows and heifers, respectively. These results are consistent with those reported in the literature.

Unicellular eukaryotes of the Trypanosomatidae family include human and animal pathogens that belong to the Trypanosoma and Leishmania genera. Diagnosis of the diseases they cause requires the sampling of body fluids (e.g., blood, lymph, peritoneal fluid, cerebrospinal fluid) or organ biopsies (e.g., bone marrow, spleen), which are mostly obtained through invasive methods. Body fluids or appendages can be alternatives to these invasive biopsies but appropriateness remains poorly studied. To further address this question, we perform a systematic review on clues evidencing the presence of parasites, genetic material, antibodies, and antigens in body secretions, appendages, or the organs or proximal tissues that produce these materials. Paper selection was based on searches in PubMed, Web of Science, WorldWideScience, SciELO, Embase, and Google. The information of each selected article (n = 333) was classified into different sections and data were extracted from 77 papers. The presence of Trypanosomatidae parasites has been tracked in most of organs or proximal tissues that produce body secretions or appendages, in naturally or experimentally infected hosts. The meta-analysis highlights the paucity of studies on human African trypanosomiasis and an absence on animal trypanosomiasis. Among the collected data high heterogeneity in terms of the I2 statistic (100%) is recorded. A high positivity is recorded for antibody and genetic material detection in urine of patients and dogs suffering leishmaniasis, and of antigens for leishmaniasis and Chagas disease. Data on conjunctival swabs can be analyzed with molecular methods solely for dogs suffering canine visceral leishmaniasis. Saliva and hair/bristles showed a pretty good positivity that support their potential to be used for leishmaniasis diagnosis. In conclusion, our study pinpoints significant gaps that need to be filled in order to properly address the interest of body secretion and hair or bristles for the diagnosis of infections caused by Leishmania and by other Trypanosomatidae parasites.

International audience; 1- Sequential damage by attackers is hypothesized to result in reciprocal antagonism (crosstalk) between jasmonic acid (JA) and salicylic acid (SA) defence signalling pathways in plants. However, evidence for this crosstalk is not universal and several studies have found positive interactions (i.e. synergism) or no interaction whatsoever between JA and SA pathways. 2- Here we conducted a meta‐analysis of studies on plant‐mediated effects of initial attackers on performance of subsequent attackers to test the hypothesis of crosstalk between plant hormonal signalling pathways. 3- We found a significant negative mean effect size of JA‐inducing initial attackers on both JA‐ and SA‐inducing subsequent attackers, but a non‐significant effect of SA‐inducing initial attackers on both JA‐ and SA‐inducing subsequent attackers. Effects on subsequent herbivores were contingent on the biology of the initial attacker, with negative effects of JA‐inducing initial herbivores but no effect of SA‐inducing initial herbivores, whereas pathogens on average did not influence subsequent herbivores. Furthermore, negative effects of JA‐inducing initial herbivores on JA‐ and SA‐inducing subsequent herbivores held when both attacks occurred on the same plant part, but when attacks were on different plant parts the effect of JA‐inducing herbivores on SA‐inducing herbivores was non‐significant. 4- Synthesis. These results indicate that reciprocal antagonism between jasmonic acid and salicylic acid pathways is not universal, and suggest assymetries and specificity in the strength of plant‐mediated interactions.

International audience

Herbivores may directly or indirectly affect plant attractiveness to pollinators. Although several studies have reported on these effects, there is yet no general consensus on the strength and sign of such interactions or their contingency on herbivory features such as the plant tissue attacked. We performed a meta-analysis of studies testing for effects of herbivores on floral traits, plant attractiveness to pollinators, and plant reproductive success. We also assessed whether herbivore effects depended on the plant tissue attacked by herbivores and if real or simulated herbivory was used. We found an overall significant negative effect of herbivores on floral traits, plant attractiveness to pollinators, and plant reproductive success. These effects were, however, contingent on the plant tissue attacked and on whether real or simulated damage was used. Real floral and leaf, but not root, herbivores showed detrimental effects on floral traits and plant attractiveness to pollinators. In addition, real leaf, but not floral or root herbivory, lowered plant reproductive success. Contrastingly, simulated leaf and floral herbivory showed no effect on any of the response variables. These findings help move forward our understanding of the strength and directionality of herbivore effects on plant attractiveness to pollinators and their underlying mechanisms., This research was financially supported by a Regional Government of Galicia Grant (IN607D 2016/001), a Spanish National Research Grant (AGL2015‐70748‐R), and the Ramón y Cajal Research Programme (RYC‐2013‐13230) to XM.

Pesticides are used worldwide in farming activities to guarantee crop yields. In southeastern Mexico, groundwater is the primary source of water for humankind. However, because of the soil characteristics and of intensive agricultural practices, the aquifer is vulnerable to pollution as shown by the regular detection of pesticide residues in groundwater. Within this context, the dissipation and adsorption of four of most used pesticides (2,4-D, atrazine, diazinon, and glyphosate) by farmers in southeastern Mexico were studied to determine their fate in agricultural soil and estimate their risk for the aquifer. Forty-one days after their application, the four pesticides were entirely dissipated from the soil. 2,4-D and glyphosate were the most persistent according to DT50. Diazinon was the most adsorbed to the soil at equilibrium time. All pesticides were volatilized in substantial amounts, reaching 10.1, 22.3, 22.4, and 43.4% of initial amount 72 h after application of glyphosate, atrazine, 2,4-D, and diazinon, respectively. Volatilization was dependent on time and pesticide type (P < 0.05). Following their K-OC, diazinon and glyphosate were found to be the most prone to leach. Therefore, in the absence of mitigation measures, their use represents a significant threat for the groundwater in Southeastern Mexico.

Elevational gradients have been highly useful for understanding the underlying forces driving variation in plant traits and plant-insect herbivore interactions. A widely held view from these studies has been that greater herbivory under warmer and less variable climatic conditions found at low elevations has resulted in stronger herbivore selection on plant defences. However, this prediction has been called into question by conflicting empirical evidence, which could be explained by a number of causes such as an incomplete assessment of defensive strategies (ignoring other axes of defence such as defence inducibility) or unaccounted variation in abiotic factors along elevational clines. We conducted a greenhouse experiment testing for inter-specific variation in constitutive leaf chemical defences (phenolic compounds) and their inducibility in response to feeding by gypsy moth larvae (Lymantria dispar L., Lepidoptera) using saplings of 18 oak (Quercus, Fagaceae) species. These species vary in their elevational distribution and together span >2400 m in elevation, therefore allowing us to test for among-species elevational clines in defences based on the elevational range of each species. In addition, we further tested for elevational gradients in the correlated expression of constitutive defences and their inducibility and for associations between defences and climatic factors potentially underlying elevational gradients in defences. Our results showed that oak species with high elevational ranges exhibited a greater inducibility of phenolic compounds (hydrolysable tannins), but this gradient was not accounted for by climatic predictors. In contrast, constitutive defences and the correlated expression of constitutive phenolics and their inducibility did not exhibit elevational clines. Overall, this study builds towards a more robust and integrative understanding of how multivariate plant defensive phenotypes vary along ecological gradients and their underlying abiotic drivers.

8 páginas, 2 figuras, 1 tabla, Aim: Research on plant–herbivore interactions has shown that islands typically have low abundances and diversity of herbivores because of barriers to dispersal, isolation and reduced land area. Islands commonly have lower levels of herbivory relative to mainland regions, and, as a consequence, insular plants should exhibit lower levels of defences than their mainland counterparts. Despite these predictions, there are significant gaps in our understanding of insularity effects on plant–herbivore interactions. For instance, most work addressing the effects of insularity on plant–herbivore interactions have compared one or a few islands with a single mainland site. In addition, studies have measured herbivory or plant defences but not both, and the influence of abiotic factors has been neglected. Location: Mediterranean Basin (from Spain to Greece). Taxon: Quercus ilex L. Methods: We conducted a large-scale study to investigate whether insect leaf herbivory and plant chemical defences in holm oak (Quercus ilex L.) differ between insular versus mainland populations. We further investigated mechanisms by which insularity effects on herbivory may take place by assessing the influence of defences and climatic variables on herbivory. Results: We found that insular populations exhibited lower herbivory and higher defences (condensed tannins) than their mainland counterparts. Our analyses, however, suggest that these concomitant patterns of insect herbivory and plant defences were seemingly unrelated as island versus mainland differences in defences did not account for the observed pattern in herbivory. Furthermore, climatic factors did not explain insularity effects on either herbivory or plant defences. Main conclusions: Overall, this study provides one of the most robust assessments to date on insularity effects on herbivory and builds towards a better understanding of the ecology and evolution of plant–insect interactions in insular ecosystems., This research was financially supported by a Spanish National Research Grant (AGL2015‐70748‐R), a Regional Government of Galicia Grant (IN607D 2016/001) and the Ramón y Cajal Research Programme (RYC‐2013‐13230) to XM.

International audience; no hay resumen

Con motivo del recién quinto centenario de la llegada de Francisco Hernández de Córdoba a Yucatán, el autor repasa la historia de ese primer encuentro «oficial» de los españoles con los mayas en tierras yucatecas en 1517, y se decanta por apoyar la idea del sitio de Cabo Catoche como punto geográfico de arribo, en apoyo a la crónica de Bernal Díaz del Castillo sobre ese pasaje. Tras el proceso de conquista, los hispanos se avecinaron en la región y con ello se inició una transformación en el paisaje biocultural de la región, tema hasta ahora poco abordado por los investigadores en Yucatán. Las menciones acerca de animales, plantas y árboles introducidos a la región, señalados en el texto de Diego de Landa, de 1566 y la Relación de Sucopo, de 1579, permiten conocer al lector el proceso de cambio que se suscitaba con la intención de europeizar el paisaje en el siglo XVI.

International audience; Application: Mesquite pods are an alternative feed to provide energy and protein to small ruminants in arid regions.Introduction: Small ruminant producers in arid regions use mesquite pods to provide their herds with a low-cost source of energy and protein and improve production parameters. This legume is a good source of protein, fiber, and sugars, which makes it a good ingredient for nutrition [2]. Furthermore, mesquite pods are rich in secondary bioactive substances such as alkaloids, flavonoids, terpenes and phenolic compounds [1]. Results of studies that assess effects of mesquite pods inclusion on ruminant rations has been inconsistent. The aim of this study was to carry out an analytical review to quantify the effect of the inclusion of mesquite pods on productive performance and methane (CH4) emissions of small ruminants.Materials and methods: A compressive and structured search of articles was performed using the following electronic databases: Google Scholar, Primo (UAEH), PubMed, Science Direct and ISI Web of Knowledge. The final database includes 37 studies with the following response variables: dry matter intake (DMI), average daily gain (ADG), feed conversion rate (FCR), digestibility (g/100 g DM) and CH4. To explore heterogeneity, a meta-regression analysis was performed, including the following a priori selected explanatory variables: dosage, species (goat and sheep), breed, body weight and processing of mesquite pods (ground, chopped, extract and meal). All analyses were performed in the R environment for statistical computing using the ‘meta’ package [3].Results: Inclusion of mesquite pods in small ruminant rations had a positive effect on ADG (+9.94 g/d), digestibility (1.45 g/100 g DM), FCR (-21.2 g/g) and DMI (+68.57 g/d)(Figure 1). The heterogeneity was substantial to all variables (>80.4), however, this was reduced when the explanatory variables were added to mixed-effect model. An increase of CH4 emission was observed to mesquite group (+0.57 g/d), likely associated with better DMI and digestibility of supplemented animals. There was a positive relationship between level of incorporation of mesquite pods and DMI, ADG and CH4 response. The largest effect size to DMI in response to mesquite supplementation was observed to meal processing following by ground and extract (Figure 2).

Nowadays, theoretical calculation tools have become powerful in predicting the behavior of corrosion inhibitors on the surface of metals and, therefore, avoiding energy consumption and the cost of experimental tests. This work aims to predict the inhibitory power of some furan derivatives on Cu (111), Fe (110), Al (111) and Sn (111) surfaces in acidic media. For this purpose, three furan derivatives&mdash, furan-2-carbaldehyde (FF1), 5-(hydroxymethyl)furfural (FF2) and 5-(hydroxymethyl)furoic acid (FF3)&mdash, have been selected to compare their intrinsic properties against corrosion as well as their behavior on iron (Fe), copper (Cu), aluminum (Al) and tin (Sn) surfaces in acid medium. Typically, the anti-corrosive properties of FF1, FF2 and FF3 were studied by using quantum chemical calculations and Monte Carlo simulations. Density Functional Theory (DFT), lowest unoccupied (ELUMO) and highest occupied (EHOMO) molecular orbital energies, energy gap (∆E), chemical hardness (&eta, ), softness (&sigma, ), electronegativity (&chi, ), electrophilicity (&omega, ) and nucleophilicity (&epsilon, ) have been calculated and discussed. Theoretical vibrational spectra were also calculated to exhibit the functional groups in the selected chemicals. On the other hand, the adsorption behaviors of FF1, FF2 and FF3 were studied on the Fe(110), Cu(111), Al(111) and Sn(111) surfaces. As a result, the adsorption energies of all molecules are ordered as Fe(110) &lt, Cu(111) &lt, Al(111) &lt, Sn(111) and FF3 seems to be more effective as a corrosion inhibitor due to the existence of both carboxylic acid and hydroxyl groups, which consist of favorable sites of adsorption into the metal surface.

International audience; A long-standing paradigm in ecology holds that herbivore pressure and thus plant defences increase towards lower latitudes. However, recent work has challenged this prediction where studies have found no relationship or opposite trends where herbivory or plant defences increase at higher latitudes. Here we tested for latitudinal variation in herbivory, chemical defences (phenolic compounds), and nutritional traits (phosphorus and nitrogen) in leaves of a long-lived tree species, the English oak Quercus robur. We further investigated the underlying climatic and soil factors associated with such variation. Across 38 populations of Q. robur distributed along an 18 degrees latitudinal gradient, covering almost the entire latitudinal and climatic range of this species, we observed strong but divergent latitudinal gradients in leaf herbivory and leaf chemical defences and nutrients. As expected, there was a negative relationship between latitude and leaf herbivory where oak populations from lower latitudes exhibited higher levels of leaf herbivory. However, counter to predictions there was a positive relationship between leaf chemical defences and latitude where populations at higher latitudes were better defended. Similarly, leaf phosphorus and nitrogen increased with latitude. Path analysis indicated a significant (negative) effect of plant chemical defences (condensed tannins) on leaf herbivory, suggesting that the latitudinal gradient in leaf herbivory was driven by an inverse gradient in defensive investment. Leaf nutrients had no independent influence on herbivory. Further, we found significant indirect effects of precipitation and soil porosity on leaf herbivory, which were mediated by plant chemical defences. These findings suggest that abiotic factors shape latitudinal variation in plant defences and that these defences in turn underlie latitudinal variation in leaf herbivory. Overall, this study contributes to a better understanding of latitudinal variation in plant-herbivore interactions by determining the identity and modus operandi of abiotic factors concurrently shaping plant defences and herbivory.