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[Context] Global change is leading to more frequent and intense tree damage and mortality events. Drought-induced tree mortality is occurring worldwide leading to broad-scale events, but the spatial patterns of tree damage and mortality, their underlying drivers and their variation over time is largely unknown., [Objectives] We investigated the spatial patterns of tree damage and mortality across Mediterranean forests of the Iberian Peninsula, the underlying effects of stand structure and climate, and how the spatial patterns and relationships with underlying drivers changed over time., [Methods] We used the Spanish Forest Inventory to analyse the autocorrelation in tree damage and mortality across forest types, hurdle-gamma models to quantify the effect of stand structure and climate on tree damage and mortality, and cross-correlograms to assess their spatial dependence and its change over time., [Results] We observed a greater magnitude and a stronger autocorrelation in tree damage than mortality, with positive aggregation up to 20 kms. There was a spatial dependence between tree damage and mortality with their drivers, with spatial aggregation increasing with water availability, drought intensity and stand structure. The spatial dependence of tree damage and mortality with the underlying drivers increased over time, particularly for drought intensity., [Conclusions] Our results suggest that the combined effect of intense competition and drought could favour more extensive die-off and tree mortality events, providing key information for identifying vulnerable areas and the planning of adaptation measures.

The replacement of fossil-derived plastics with those obtained from bio-based resources, which present suitable performance to be employed as commodity plastics is currently an important field of research, given the urgent need to transition from a fossil-based to a more sustainable economy. In this context, this work is focused on the application of a catalytic system based on silsesquioxane-cyclopentadienyl titanium complexes for the preparation of bio-based polymers, which can be used as additives to improve the poor material properties of a biodegradable polymer such as poly(lactic acid) (PLA). These titanium complexes, when activated with methylaluminoxane or with triflate salts, are shown to be capable of the polymerization of two bio-based monomers: myrcene and anethole. It is notable that polymerizations with these two distinct monomers take place through different mechanisms. The resulting polymyrcene (PMy) and polyanethol (PAN) have been applied as modifiers for PLA. Binary blends of PMy and PLA exhibited a considerable decrease in T and the promotion of PLA crystallization for a PMy content below 15 wt%. The mechanical properties of the PLA/PMy blends also displayed plasticization, with a decrease in the elastic modulus and enhanced plasticity, which resulted in less fragile systems compared to pure PLA. Morphological analysis has indicated a partially miscible, phase-separated system with micron-sized domains. In contrast, PAN completely inhibited PLA crystallization and the PLA/PAN blends were immiscible, but well-dispersed, a phase-separated system was obtained in solvent-casting film preparation with very small PAN domains. The blends showed higher tensile modulus than pure PLA and an absence of plastic behaviour, resulting in more fragile systems upon the addition of PAN to PLA.

This work presents a revision of the main applications of capillary electromigration (CE) methods in food analysis and Foodomics. Papers that were published during the period March 2021 to March 2023 are included. The work shows the multiple CE methods that have been developed and applied to analyze different types of molecules in foods and beverages. Namely, CE methods have been applied to analyze amino acids, biogenic amines, heterocyclic amines, peptides, proteins, phenols, polyphenols, pigments, lipids, carbohydrates, vitamins, DNAs, contaminants, toxins, pesticides, additives, residues, small organic and inorganic compounds, and other minor compounds. In addition, new CE procedures to perform chiral separation and for evaluating the effects of food processing as well as the last developments of microchip CE and new applications in Foodomics will be also discussed. The new procedures of CE to investigate food quality and safety, nutritional value, storage, and bioactivity are also included in the present review work.

info:eu-repo/semantics/nonPublished

Selective and brain-permeable protein kinase inhibitors are in preclinical development for treating neurodegenerative diseases. Among them, MLK3 inhibitors, with a potent neuroprotective biological action have emerged as valuable agents for the treatment of pathologies such as Alzheimer's, Parkinson's disease and amyotrophic lateral sclerosis. In fact, one MLK3 inhibitor, CEP-1347, reached clinical trials for Parkinson's disease. Additionally, another compound called prostetin/12k, a potent and rather selective MLK3 inhibitor has started clinical development for ALS based on its motor neuron protection in both in vitro and in vivo models. In this review, we will focus on the role of MLK3 in neuron-related cell death processes, neurodegenerative diseases, and the potential advantages of targeting this kinase through pharmacological modulation for neuroprotective treatment.

Historical cartography continues to be an essential resource in developing strategies for the analysis of anthropised landscapes. In recent years, the digitisation and conversion of a large number of pre-20th-century maps to modern geographic coordinate systems and data hierarchisation in GIS-integrated databases have opened up huge possibilities. In this paper, we highlight some of the advantages and issues that we observed in using historical cartography in the Iberian Peninsula heartlands by comparing archaeological data, textual sources and maps and various levels of information obtained for our area of study: the southeast of the present-day province of Guadalajara, Spain. Using the longue durée approach conceptualised by Fernand Braudel (1976), historical cartography enabled us to delve deeper into two essential aspects, land use and the road network, while providing elements of diachrony that suggest changes in the landscape in specific and disruptive periods.

This work examines the possible behaviour of Neanderthal groups at the Cueva Des-Cubierta (central Spain) via the analysis of the latter’s archaeological assemblage. Alongside evidence of Mousterian lithic industry, Level 3 of the cave infll was found to contain an assemblage of mammalian bone remains dominated by the crania of large ungulates, some associated with small hearths. The scarcity of post-cranial elements, teeth, mandibles and maxillae, along with evidence of anthropogenic modifcation of the crania (cut and percussion marks), indicates that the carcasses of the corresponding animals were initially processed outside the cave, and the crania were later brought inside. A second round of processing then took place, possibly related to the removal of the brain. The continued presence of crania throughout Level 3 indicates that this behaviour was recurrent during this level’s formation. This behaviour seems to have no subsistence-related purpose but to be more symbolic in its intent.

Previous attempts to quantify tree abundance at global scale have largely neglected the role of local competition in modulating the influence of climate and soils on tree density. Here, we evaluated whether mean tree size in the world’s natural forests alters the effect of global productivity on tree density. In doing so, we gathered a vast set of forest inventories including >3000 sampling plots from 23 well-conserved areas worldwide to encompass (as much as possible) the main forest biomes on Earth. We evidence that latitudinal productivity patterns of tree density become evident as large trees become dominant. Global estimates of tree abundance should, therefore, consider dependencies of latitudinal sources of variability on local biotic influences to avoid underestimating the number of trees on Earth and to properly evaluate the functional and social consequences.

Novel species of fungi described in this study include those from various countries as follows: Australia, Aschersonia mackerrasiae on whitefly, Cladosporium corticola on bark of Melaleuca quinquenervia, Penicillium nudgee from soil under Melaleuca quinquenervia, Pseudocercospora blackwoodiae on leaf spot of Persoonia falcata, and Pseudocercospora dalyelliae on leaf spot of Senna alata. Bolivia, Aspicilia lutzoniana on fully submersed siliceous schist in high-mountain streams, and Niesslia parviseta on the lower part and apothecial discs of Erioderma barbellatum onatwig. Brazil, Cyathus bonsai on decaying wood, Geastrum albofibrosum from moist soil with leaf litter, Laetiporus pratigiensis on a trunk of a living unknown hardwood tree species, and Scytalidium synnematicum on dead twigs of unidentified plant. Bulgaria, Amanita abscondita on sandy soil in a plantation of Quercus suber. Canada, Penicillium acericola on dead bark of Acer saccharum, and Penicillium corticola on dead bark of Acer saccharum. China, Colletotrichum qingyuanense on fruit lesion of Capsicum annuum. Denmark, Helminthosphaeria leptospora on corticioid Neohypochnicium cremicolor. Ecuador (Galapagos), Phaeosphaeria scalesiae on Scalesia sp. Finland, Inocybe jacobssonii on calcareouss oils in dry forests and park habitats. France, Cortinarius rufomyrrheus on sandy soil under Pinus pinaster, and Periconia neominutissima on leaves of Poaceae. India, Coprinopsis fragilis on decaying bark of logs, Filoboletus keralensis on unidentified woody substrate, Penicillium sankaranii from soil, Physisporinus tamilnaduensis on the trunk of Azadirachta indica, and Poronia nagaraholensis on elephant dung. Iran, Neosetophoma fic on infected leaves of Ficus elastica. Israel, Cnidariophoma eilatica (incl. Cnidariophoma gen. nov.) from Stylophora pistillata. Italy, Lyophyllum obscurum on acidic soil. Namibia, Aureobasidium faidherbiae on dead leaf of Faidherbia albida, and Aureobasidium welwitschiae on dead leaves of W

[Introduction]: Drought-associated tree mortality has been increasing worldwide since the last decades, impacting structure and functioning of forest ecosystems, with implications for energy, carbon and water fluxes. However, the understanding of the individual vulnerability to drought-induced mortality is still limited., [Methods]: We aimed to identify the factors that triggered the mortality of the widely distributed Pinus sylvestris L. in an extensive forest area in central Spain. We compared radial growth patterns in pairs of alive and recently dead individuals that co-occur in close proximity and present similar age and size, thereby isolating the effects of size and environment from the mortality process. Temporal dynamics of growth, growth synchrony, and growth sensitivity to water availability (precipitation minus potential evapotranspiration) were compared between alive and recently dead trees., [Results and discussion]: Over the last 50 years, although we did not detect significant differences in growth between alive and dead trees, an increase in the growth synchrony and sensitivity to water availability (i.e. slope of the climatic water balance in the growth model) was observed in all trees as drought intensity increased. 20 years before mortality, dead individuals showed lower growth synchrony and growth sensitivity to water availability than alive ones, without significant differences in growth. Recorded reduction in growth synchrony and growth sensitivity to water availability in dead trees suggests a decoupling between tree growth and climate, which could increase the risk of hydraulic failure and/or carbon starvation under increasingly arid conditions. Thus, the use of reduced growth sensitivity to water availability as potential early-warning signal of tree mortality, together with reduced growth synchrony, should be further explored, particularly in pine species in seasonally dry areas.

Increased winter and early spring temperatures due to climate change can enhance forest productivity due to earlier growth onset in temperate regions. However, drought-prone forests can be highly vulnerable to the combined effect of high summer temperatures and water stress. Understanding how water stress and rising temperatures along the growing season control tree growth in co-occurring species with contrasting drought tolerances is key to project climate change effects on forest dynamics. Here, we evaluated the interactive effect of seasonal temperature and water availability on annual radial growth and climate–growth instability over six decades (1951–2015) in three pine species with contrasting drought tolerances (Pinus pinaster, Pinus nigra, and Pinus sylvestris). For this, we retrospectively observed radial tree growth using dendroecological methods and evaluated seasonal temperature and water availability effects by using linear mixed models. Early growing season temperature and water availability had a positive effect on tree growth, but the positive effect of late season temperature was modulated by water availability. Moving time-window analyses revealed temporal instability in climate–growth relationships. Since the 1980s, pine species showed a higher growth sensitivity to both seasonal temperatures and annual water availability. Furthermore, growth reductions were more pronounced due to increased summer temperatures and reduced precipitation. Our results were similar for the three studied pine species despite their contrasting tolerance to drought. Overall, climate warming effects on pine growth are contingent upon water availability in Mediterranean continental forests. Synchronization among species, climate–growth instability, and negative growth trends suggests an increased vulnerability to drought of Mediterranean pine species in response to ongoing climate change.

This work is focused on the study of the metabolomic changes that occur in cocoa powder throughout the alkalization process by using an untargeted ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-QTOF-MS) metabolomics approach. With this aim, cocoa powder samples submitted to different alkalization degrees (light, medium, and strong) were analyzed. Metabolite extraction was performed using 75% MeOH in water since it provided the highest number of molecular features. After data processing, non-supervised and supervised methods were applied to carry out the statistical data analysis. Thus, the most significant metabolites that allow establishing differences among the cocoa powder samples submitted to the alkalization processes were pointed out. Thus, 43 and 30 metabolites in positive and negative ionization modes, respectively, demonstrated to be relevant. Among them, 9 compounds were unequivocally identified and 22 tentatively identified. Most of them were amino acids, alkaloids, organic acids or polyphenols, among others.

A sedimentary sequence covering the entire last glacial cycle (period between Terminations I and II) outcrops along the south-eastern coast of Formentera Island. A detailed geomorphological, geological and sedimentological study, supported by geochemical, soil and soil-morphology analyses, magnetic susceptibility, phytolite content and luminescence dating (TL, OSL) allowed to reconstruct the environmental evolution of this coastal setting, and to frame it within the evolutionary pattern of the North Atlantic climate variability. Three highstands of sea level are identified in this island for MIS 5e, and a fourth one is attributed to MIS5a. MIS5 – MIS4 transition is characterized by soil development under a moist-warm climate and a descending sea level scenario. Aeolian units (72 ± 7 ka BP) developed during MIS4 under prevailing northerly winds that persisted until the beginning of MIS3, when new aeolian dunes (54 ± 5 ka BP) developed after a major sea-level lowstand. A sudden shift in prevailing winds occur within MIS3, when aeolian units (51 ± 4 ka BP) grew under the influence of S-SW winds and moister climate, evidenced by a dense root bioturbation. The greater influence of northerly winds is attributed to the weakening of Atlantic Meridional Overturning Current (AMOC) in North Atlantic, and enhancement of westerlies in NW Europe during colder periods. Periods of prevailing southerly winds and moister climate correlate well with warm Greenland Interstadials, and reinforcement of AMOC. Between 50 and 40ka, alluvial/colluvial sedimentary units punctuated by soil and calcrete development, witness the climatic variability recorded along this period in the North Atlantic. A sedimentary hiatus with erosion and calcrete development characterizes the transit between MIS3 and MIS2. Finally, a reddish alluvial sedimentary unit records a short span of humid and warm climate (soil development, peak in magnetic susceptibility, phytolite content) within MIS 2 (20 ± 2 ka BP – 17

Elisabeth S. Vrba’s resource-use hypothesis suggests that speciation in biomes subjected to successive expansion-contraction-fragmentation during periods of climatic change generates high frequency of species restricted to a single biome (stenobiomic species). We compiled biome occupation for all terrestrial mammals and, using Monte Carlo simulations, demonstrated that patterns of biome occupation are congruent with those predicted by the resource-use hypothesis. Biome specialists are much more speciose than expected by chance, while there are fewer moderate biome generalists than expected. Despite their scarcity, extreme eurybiomic lineages show significant overrepresentation, which suggests they are seldom affected by climate-related extinction processes. Additionally, stenobiomic species are concentrated in biomes placed at the extremes of the climatic gradient, such as equatorial rainforest, subtropical desert, steppe, and tundra. Although this pattern is fairly maintained across different mammalian families, highlighting its universality, our analysis also found great variability. Exceptions to the predictions of the resource-use hypothesis seem to be associated to biome climatic or geographical heterogeneity, which favours vicariance in some biomes not placed in extremes of the climatic gradient (tropical deciduous woodland, sclerophyllous woodland-shrubland), as well as life-history differences across taxa, which generates a stronger trend to specialisation in small body size lineages than in larger mammals.

Durum wheat (Triticum turgidum, 2n = 4x = AABB) includes several subspecies with differential characteristics in their root system architecture (RSA). Subspecies durum has longer and more vertical roots, while subspecies turgidum has smaller and shallower roots. The homeologous genes TtDro1A and TtDro1B of both subspecies have been identified and found to differ in their sizes, sequences and the proteins they encode. To determine whether there is a relationship between the level of expression of these two genes and the angle adopted by the roots of durum wheat seedlings, their expressions has been studied by RT-qPCR, both in the primary seminal root and in the other seminal roots. The results of the analyses showed that the TtDro1A gene is expressed 1.4 times more in the primary seminal root than in the other seminal roots. Furthermore, this gene is expressed 2.49 to 8.76 times more than TtDro1B depending on root type (primary or seminal) and subspecies. There are positive correlations between the expression ratio of both genes (TtDro1A/TtDro1B) and the mean of all root angles, the most vertical root angle and the most horizontal root angle of the seedlings. The higher the expression of TtDro1B gene, the lower the root growth angles.

El cambio climático reciente, independientemente de los tipos y contribución relativa de las distintas causas antrópicas/naturales responsables, está motivando impactos medibles sobre numerosos aspectos de la biología de las aves, como son la distribución, abundancia, fenología, migración y éxito reproductivo (McCarty 2001; Cotton 2003; Parmesan y Yohe 2003; Sanz et al. 2003; Gordo et al. 2005; Leech y Crick 2007; Carey 2009; Stephens et al. 2016; Lehikoinen et al. 2019; Lehikoinen et al. 2021, McLeana et al. 2022). Estos efectos no son generalizables a través de diferentes especies, ya que dependen de sus preferencias climáticas, de hábitat, de su migratoriedad o de sus patrones de distribución a escala continental (Archaux 2004; Hitch y Leberg 2007; La Sorte y Thompson 2007; Seoane y Carrascal 2008; Mason et al. 2019; Viana y Chase 2022). Esta heterogeneidad interespecífica observada en las respuestas al cambio climático reciente plantea la necesidad de conocer cómo se verá afectada la biodiversidad aviar en los próximos años, de perdurar en el tiempo la tendencia registrada de cambio climático global. Este ejercicio tiene una considerable utilidad aplicada, permitiendo una evaluación de la situación tanto a nivel taxonómico como espacial.

A recently reported combined polymerization process of glycidyl methacrylate, mediated by homometallic and heterobimetallic aluminium complexes, naturally produces nano-sized macromolecular assemblies. In this work, the morphological features and the rheological properties of these novel nanoassemblies are studied. The hydrodynamic sizes of the nanoparticles in the solution range from 10 to 40 nm (in numbers), but on a flat surface they adopt a characteristic thin disk shape. The dynamic moduli have been determined in a broad range of temperatures, and the time—temperature superposition applied to obtain master curves of the whole viscoelastic response from the glassy to the terminal regions. The fragility values obtained from the temperature dependence are of m ~40, typical of van de Waals liquids, suggesting a very effective packing of the macromolecular assemblies. The rheological master curves feature a characteristic viscoelastic relaxation with the absence of elastic intermediate plateau, indicating that the systems behaved as un-entangled polymers. The analysis of the linear viscoelastic fingerprint reveals a Zimm-like dynamics at intermediate frequencies typical of unentangled systems. This behaviour resembles that observed in highly functionalized stars, dendrimers, soft colloids and microgels.

Fifteen pyridazino-pyrrolo-quinoxalinium salts were synthesized and tested for their antiprotozoal activity against Leishmania infantum amastigotes. Eleven of them turned out to be leishmanicidal, with EC values in the nanomolar range, and displayed low toxicity against the human THP-1 cell line. Selectivity indices for these compounds range from 10 to more than 1000. Compounds 3b and 3f behave as potent inhibitors of the oxidoreductase activity of the essential enzyme trypanothione disulfide reductase (TryR). Interestingly, binding of 3f is not affected by high trypanothione concentrations, as revealed by the noncompetitive pattern of inhibition observed when tested in the presence of increasing concentrations of this substrate. Furthermore, when analyzed at varying NADPH concentrations, the characteristic pattern of hyperbolic uncompetitive inhibition supports the view that binding of NADPH to TryR is a prerequisite for inhibitor-protein association. Similar to other TryR uncompetitive inhibitors for NADPH, 3f is responsible for TryR-dependent reduction of cytochrome c in a reaction that is typically inhibited by superoxide dismutase.

Drought-induced forest dieback can lead to a tipping point in community dominance, but the coupled response at the tree and stand-level response has not been properly addressed. New spatially and temporally integrated monitoring approaches that target different biological organization levels are needed. Here, we compared the temporal responses of dendrochronological and spectral indices from 1984 to 2020 at both tree and stand levels, respectively, of a drought-prone Mediterranean Pinus pinea forest currently suffering strong dieback. We test the influence of climate on temporal patterns of tree radial growth, greenness and wetness spectral indices; and we address the influence of major drought episodes on resilience metrics. Tree-ring data and spectral indices followed different spatio-temporal patterns over the study period (1984–2020). Combined information from tree growth and spectral trajectories suggests that a reduction in tree density during the mid-1990s could have promoted tree growth and reduced dieback risk. Additionally, over the last decade, extreme and recurrent droughts have resulted in crown defoliation greater than 40% in most plots since 2019. We found that tree growth and the greenness spectral index were positively related to annual precipitation, while the wetness index was positively related to mean annual temperature. The response to drought, however, was stronger for tree growth than for spectral indices. Our study demonstrates the value of long-term retrospective multiscale analyses including tree and stand-level scales to disentangle mechanisms triggering and driving forest dieback.

Tree-ring data has been widely used to inform about tree growth responses to drought at the individual scale, but less is known about how tree growth sensitivity to drought scales up driving changes in forest dynamics. Here, we related tree-ring growth chronologies and stand-level forest changes in basal area from two independent data sets to test if tree-ring responses to drought match stand forest dynamics (stand basal area growth, ingrowth, and mortality). We assessed if tree growth and changes in forest basal area covary as a function of spatial scale and tree taxa (gymnosperm or angiosperm). To this end, we compared a tree-ring network with stand data from the Spanish National Forest Inventory. We focused on the cumulative impact of drought on tree growth and demography in the period 1981–2005. Drought years were identified by the Standardized Precipitation Evapotranspiration Index, and their impacts on tree growth by quantifying tree-ring width reductions. We hypothesized that forests with greater drought impacts on tree growth will also show reduced stand basal area growth and ingrowth and enhanced mortality. This is expected to occur in forests dominated by gymnosperms on drought-prone regions. Cumulative growth reductions during dry years were higher in forests dominated by gymnosperms and presented a greater magnitude and spatial autocorrelation than for angiosperms. Cumulative drought-induced tree growth reductions and changes in forest basal area were related, but initial stand density and basal area were the main factors driving changes in basal area. In drought-prone gymnosperm forests, we observed that sites with greater growth reductions had lower stand basal area growth and greater mortality. Consequently, stand basal area, forest growth, and ingrowth in regions with large drought impacts was significantly lower than in regions less impacted by drought. Tree growth sensitivity to drought can be used as a predictor of gymnosperm demographic rates in

Legacies of past climate conditions and historical management govern forest productivity and tree growth. Understanding how these processes interact and the timescales over which they influence tree growth is critical to assess forest vulnerability to climate change. Yet, few studies address this issue, likely because integrated long-term records of both growth and forest management are uncommon. We applied the stochastic antecedent modelling (SAM) framework to annual tree-ring widths from mixed forests to recover the ecological memory of tree growth. We quantified the effects of antecedent temperature and precipitation up to 4 years preceding the year of ring formation and integrated management effects with records of harvesting intensity from historical forest management archives. The SAM approach uncovered important time periods most influential to growth, typically the warmer and drier months or seasons, but variation among species and sites emerged. Silver fir responded primarily to past climate conditions (25–50 months prior to the year of ring formation), while European beech and Scots pine responded mostly to climate conditions during the year of ring formation and the previous year, although these responses varied among sites. Past management and climate interacted in such a way that harvesting promoted growth in young silver fir under wet and warm conditions and in old European beech under drier and cooler conditions. Our study shows that the ecological memory associated with climate legacies and historical forest management is species-specific and context-dependent, suggesting that both aspects are needed to properly evaluate forest functioning under climate change.

This work presents a novel low-pass filter based on using dielectric materials, in the critical areas of the structure, to prevent high-power space condition adverse effects, like multipactor breakdown. It is shown that the use of dielectric pieces in the inverters of the filter leads to increased gaps between metallic parts in the irises. In addition, by completely filling up the air space in critical gaps, the multipactor breakdown thresholds can be increased. Moreover, they also lead to a volume reduction, increasing compactness of the structure. To demonstrate the proposed concept, a prototype has been manufactured with an aluminum housing and a Teflon dielectric material, and tested for multipactor effect at ESA-VSC Laboratory. Tests confirmed that the manufactured filter has good electrical performance with cut-off frequency 13.6 GHz, insertion losses 0.32 dB, return losses better than 15 dB, and additionally does not show multipactor breakdown up to the maximum power tested in the experimental facility (5.5 kW).

Instilling segregated cationic and lipophilic domains with an angular disposition in a trehalose-based trifaceted macrocyclic scaffold allows engineering patchy molecular nanoparticles leveraging directional interactions that emulate those controlling self-assembling processes in viral capsids. The resulting trilobular amphiphilic derivatives, featuring a Mickey Mouse architecture, can electrostatically interact with plasmid DNA (pDNA) and further engage in hydrophobic contacts to promote condensation into transfectious nanocomplexes. Notably, the topology and internal structure of the cyclooligosaccharide/pDNA co-assemblies can be molded by fine-tuning the valency and characteristics of the cationic and lipophilic patches, which strongly impacts the transfection efficacy in vitro and in vivo. Outstanding organ selectivities can then be programmed with no need of incorporating a biorecognizable motif in the formulation. The results provide a versatile strategy for the construction of fully synthetic and perfectly monodisperse nonviral gene delivery systems uniquely suited for optimization schemes by making cyclooligosaccharide patchiness the focus.

We analysed nine traits of the root system of 223 genotypes of Triticum turgidum (2n = 4x = AABB) subspecies dicoccoides, dicoccum, turgidum, durum and polonicum, finding a large intra and interspecific variability in both the number and size of roots, as well as in their spatial distribution. We studied the presence of an incomplete MITE (Miniature Inverted-repeat Transposable Element) inserted in the TtDro1B gene, which is present in some genotypes of dicoccoides, dicoccum, and turgidum, but not in polonicum and the 97.9% of the durum accessions. Comparison between genotypes shows that genotypes with the MITE element have smaller and shallower roots. Since Aegilops is considered to be the donor of the wheat B genome, the presence of the same MITE element was analysed in 55 accessions of the species Aegilops speltoides, searsii, bicornis and longissima, and in no case was it detected. We propose that after the emergence of T. turgidum subsp. dicoccoides, the insertion of the MITE element probably occurred in a single plant. Subsequent domestication resulted in genotypes of dicoccum with and without the MITE element, which after selection gave rise to the subspecies turgidum, and durum and polonicum, respectively. The MITE element can be used to differentiate turgidum from the durum and polonicum with high reliability.

Pines are the dominant conifers in Mediterranean forests. As long-lived sessile organisms that seasonally have to cope with drought periods, they have developed a variety of adaptive responses. However, during last decades, highly intense and long-lasting drought events could have contributed to decay and mortality of the most susceptible trees. Among conifer species, Pinus pinaster Ait. shows remarkable ability to adapt to different environments. Previous molecular analysis of a full-sib family designed to study drought response led us to find active transcriptional activity of stress-responding genes even without water deprivation in tolerant genotypes. To improve our knowledge about communication between above- and below-ground organs of maritime pine, we have analyzed four graft-type constructions using two siblings as rootstocks and their progenitors, Gal 1056 and Oria 6, as scions. Transcriptomic profiles of needles from both scions were modified by the rootstock they were grafted on. However, the most significant differential gene expression was observed in drought-sensitive Gal 1056, while in drought-tolerant Oria 6, differential gene expression was very much lower. Furthermore, both scions grafted onto drought-tolerant rootstocks showed activation of genes involved in tolerance to abiotic stress, and is most remarkable in Oria 6 grafts where higher accumulation of transcripts involved in phytohormone action, transcriptional regulation, photosynthesis and signaling has been found. Additionally, processes, such as those related to secondary metabolism, were mainly associated with the scion genotype. This study provides pioneering information about rootstock effects on scion gene expression in conifers.

La tercera edición de la Tabla Cronoestratigráfica del Cuaderno de la península ibérica (v 3.0) sustituye a las versiones más antiguas editadas en 2007 (v 1.0) y 2009 (v 2.0). Desde esta fecha la Comisión Internacional de Estratigrafía (ICS), y más concretamente la Subcomisión de Estratigrafía del Cuaternario (SQS), ha estado trabajando tanto en la definición del Periodo Cuaternario como en sus subdivisiones, aportando nuevos GSSP (Global Stratotype Section and Points) para los mismos. La nueva tabla incluye los GSSPs recientemente aprobados para la subdivisión de la Serie Holocena (Groenlandiense, Norgripiense y Meghalayense), así como el nuevo piso Chibaniense (774,1 - 128,0 ka) ratificado por la IUGS a principios del 2020, que sustituye al Pleistoceno medio. Esta versión 3.0 también incluye todas las modificaciones y correcciones que han surgido desde entonces, incluyendo la actualización de datos, fechas y dataciones para los yacimientos paleontológicos y/o arqueológicos más representativos de la península. Asimismo, se ha revisado la extensión de los diferentes periodos climáticos considerados para Europa central y Alpes ajustándose a las biozonas de mamíferos y las faunas asociadas. La extensión de estas últimas ha de entenderse referida a Europa central y meridional no solo a la península ibérica. En la distribución temporal de periodos de la prehistoria y complejos tecno-culturales asociados se diferencia entre el continente africano y europeo. De la misma forma la sucesión de especies de homininos se centra también en estos dos continentes. Esta revisión ofrece una lista de referencias actualizada, así como una versión digital interactiva que se implementará próximamente.

The antimicrobial lysozyme (Lys) was electrostatically incorporated to negatively charged crosslinked poly(glycidyl methacrylate) (c-PGMA) macromolecular assemblies. The resulting material was characterized by AFM, infrared spectra, water contact angle measurements and the staining with the primary amino specific dye fluorescamine. c-PGMA nanoparticles were successfully loaded with Lys reaching ratios of 27.3 ± 4.0 and 22.5 ± 1.7 mg Lys/g polymer for c-PGMA suspensions and functionalized glass substrates, respectively. Lys-loaded c-PGMA caused clear inhibition zones on S. aureus and E. coli in comparison to neat c-PGMA. c-PGMA functionalized surfaces were intrinsically resistant to colonization, but the incorporation of Lys added resistance to bacterial attachment and allowed keeping surfaces clean of bacterial cells for both strains. A relatively rapid release (24 h) of Lys was observed at physiological pH (7.4). In addition, c-PGMA functionalized substrates could be reloaded several times without losing capacity. c-PGMA macromolecular assemblies did not display cytotoxicity to human dermal fibroblasts as shown in 24 h MTT assays. This work demonstrated that c-PGMA assemblies display durable antibacterial activity, biocompatibility, and full reloading capacity with antimicrobial peptides. c-PGMA functionalized materials have potential application as nanocarriers for anti-infective uses.

The study of deep-time ecological dynamics has the ability to inform conservation decisions by anticipating the behavior of ecosystems millions of years into the future. Using network analysis and an exceptional fossil dataset spanning the past 21 million years, we show that mammalian ecological assemblages undergo long periods of functional stasis, notwithstanding high taxonomic volatility due to dispersal, speciation, and extinction. Higher functional richness and diversity promoted the persistence of functional faunas despite species extinction risk being indistinguishable among these different faunas. These findings, and the large mismatch between functional and taxonomic successions, indicate that although safeguarding functional diversity may or may not minimize species losses, it would certainly enhance the persistence of ecosystem functioning in the face of future disturbances.

Novel species of fungi described in this study include those from various countries as follows: Antartica, Cladosporium austrolitorale from coastal sea sand. Australia, Austroboletus yourkae on soil, Crepidotus innuopurpureus on dead wood, Curvularia stenotaphri from roots and leaves of Stenotaphrum secundatum and Thecaphora stajsicii from capsules of Oxalis radicosa. Belgium, Paraxerochrysium coryli (incl. Paraxerochrysium gen. nov.) from Corylus avellana. Brazil, Calvatia nordestina on soil, Didymella tabebuiicola from leaf spots on Tabebuia aurea, Fusarium subflagellisporum from hypertrophied floral and vegetative branches of Mangifera indica and Microdochium maculosum from living leaves of Digitaria insularis. Canada, Cuphophyllus bondii fromagrassland. Croatia, Mollisia inferiseptata from a rotten Laurus nobilis trunk. Cyprus, Amanita exilis oncalcareoussoil. Czech Republic, Cytospora hippophaicola from wood of symptomatic Vaccinium corymbosum. Denmark, Lasiosphaeria deviata on pieces of wood and herbaceousdebris. Dominican Republic, Calocybella goethei among grass on a lawn. France (Corsica) , Inocybe corsica onwetground. France (French Guiana) , Trechispora patawaensis on decayed branch of unknown angiosperm tree and Trechispora subregularis on decayed log of unknown angiosperm tree. [...]

Novel species of fungi described in this study include those from various countries as follows: Algeria, Phaeoacremonium adelophialidum from Vitis vinifera. Antarctica, Comoclathris antarctica from soil. Australia, Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia, Eremothecium peggii in fruit of Citrus australis, Microdochium ratticaudae from stem of Sporobolus natalensis, Neocelosporium corymbiae on stems of Corymbia variegata, Phytophthora kelmanii from rhizosphere soil of Ptilotus pyramidatus, Pseudosydowia backhousiae on living leaves of Backhousia citriodora, Pseudosydowia indoor oopillyensis, Pseudosydowia louisecottisiae and Pseudosydowia queenslandica on living leaves of Eucalyptus sp. Brazil, Absidia montepascoalis from soil. Chile, Ilyonectria zarorii from soil under Maytenus boaria. Costa Rica, Colletotrichum filicis from an unidentified fern. Croatia, Mollisia endogranulata on deteriorated hardwood. Czech Republic, Arcopilus navicularis from tea bag with fruit tea, Neosetophoma buxi as endophyte from Buxus sempervirens, Xerochrysium bohemicum on surface of biscuits with chocolate glaze and filled with jam. France, Entoloma cyaneobasale on basic to calcareous soil, Fusarium aconidiale from Triticum aestivum, Fusarium juglandicola from buds of Juglans regia. Germany, Tetraploa endophytica as endophyte from Microthlaspi perfoliatum roots. India, Castanediella ambae on leaves of Mangifera indica, Lactifluus kanadii on soil under Castanopsis sp., Penicillium uttarakhandense from soil. Italy, Penicillium ferraniaense from compost. Namibia, Bezerromyces gobabebensis on leaves of unidentified succulent, Cladosporium stipagrostidicola on leaves of Stipagrostis sp., Cymostachys euphorbiae on leaves of Euphorbia sp., Deniquelata hypolithi from hypolith under a rock, Hysterobrevium walvisbayicola on leaves of unidentified tree, Knufia hypolithi and Knufia walvisbayicola from hypolith under a rock, Lapidomyces stipagrostidicola on leave

Although mapping long-term scenarios can be considered crucial in decision-making, its inclusion in planning processes remains a challenge. This paper aims to gain insights into this by showing a participatory mapping method that creates a dialogue and interaction space between stakeholders and experts. The research was based on three 2050 visions, taking as a case study the eastern sector of the metropolitan area of Madrid, Spain. The method consisted of conducting a participatory mapping workshop involving urban planners, transport engineers, environmental consultants, and property developers. Those experts mapped transformations in urban land use for each 2050 scenario. The results evidenced differences between the three 2050 scenarios, highlighting the usefulness of the participatory mapping workshop to represent the amount and nature of growth in urban land use and organisation of the transport network.

The interior of the Iberian Peninsula has few Middle Palaeolithic sites, especially when compared to other areas of the Mediterranean Basin and the northern Spanish region. Few in number too are the zooarchaeological and taphonomic studies that throw light on the relationships between Neanderthal groups, their environment, and the use they made of it. The present work examines, both zooarchaeo-logically and taphonomically, the faunal remains of levels F and D of the Navalmaíllo Rock Shelter (Pinilla del Valle, Madrid, Spain) - the largest collection of such remains ever studied from the Iberian interior. The results allow this site to be interpreted as a Neanderthal hunting camp where occupations were short-term. Neanderthal people were the main agents that accumulated the site's faunal remains - largely those of large bovids and to a lesser extent medium-sized cervids. The activity of carnivores was also identified, but these animals mostly left behind the remains of small prey or fed upon carcasses abandoned at the camp by human hunters.

La llegada de población migrante a nuestro país incluye un número de desafíos de índole lingüístico y cultural. Es por ello que, desde las distintas administraciones públicas y organismos gubernamentales, se aúnan esfuerzos por ayudar a esta población a superar las posibles barreras que puedan llegar a dificultar una comunicación efectiva. El presente artículo analiza una guía de integración para población inmigrante en la Comunidad de Madrid y expone los resultados más destacados del análisis llevado a cabo en cuanto a comprensión y claridad de la misma. Para ello, la primera sección aborda un repaso del marco contextual del trabajo. Seguidamente, se presenta el análisis y la propuesta de simplificación de la mencionada guía, y donde se procederá a una revisión de diversos aspectos lingüísticos. Los resultados obtenidos indican que, aunque los esfuerzos son enormes por parte de las administraciones públicas a la hora de proporcionar recursos adaptados a la población migrante, los textos destinados a esta población requieren un proceso de adaptación a sus necesidades.

eEF1A1 and eEF1A2 are paralogous proteins whose presence in most normal eukaryotic cells is mutually exclusive and developmentally regulated. Often described in the scientific litera-ture under the collective name eEF1A, which stands for eukaryotic elongation factor 1A, their best known activity (in a monomeric, GTP-bound conformation) is to bind aminoacyl-tRNAs and deliver them to the A-site of the 80S ribosome. However, both eEF1A1 and eEF1A2 are endowed with mul-titasking abilities (sometimes performed by homo-and heterodimers) and can be located in different subcellular compartments, from the plasma membrane to the nucleus. Given the high sequence identity of these two sister proteins and the large number of post-translational modifications they can undergo, we are often confronted with the dilemma of discerning which is the particular pro-teoform that is actually responsible for the ascribed biochemical or cellular effects. We argue in this review that acquiring this knowledge is essential to help clarify, in molecular and structural terms, the mechanistic involvement of these two ancestral and abundant G proteins in a variety of funda-mental cellular processes other than translation elongation. Of particular importance for this special issue is the fact that several de novo heterozygous missense mutations in the human EEF1A2 gene are associated with a subset of rare but severe neurological syndromes and cardiomyopathies.

We report the design of a new model based on a small neutral 8-aryl-3-formylBODIPY and its suitability to develop privileged highly bright and photostable fluorescent probes for selective and, more importantly, covalent staining of mitochondria.

The COVID-19 lockdown has unprecedently affected the dynamics of our society. As traffic flow is a good proxy for societal activity, traffic monitoring becomes a useful tool to assess the lockdown’s impacts. Here we turned two strands of unused telecommunication fibers in Pasadena, California into a seismic array of ~5,000 sensors and detected ground vibrations caused by moving vehicles along the streets above the cable. We monitor the number of vehicles and their mean speed between December 2019 and August 2020 in high spatial and temporal resolution, and then analyze the traffic patterns change due to the COVID-19 lockdown. Our results show a city-wide decline in traffic volume and an increase in speed due to the lockdown, although the level of impact varies substantially by streets. This study demonstrates the feasibility of using telecommunication fiber optic cables in traffic monitoring, which has implications for public health, economy, and transportation safety

Herein we have reviewed our recent developments for the identification of new tacrine analogues for Alzheimer's disease (AD) therapy. Tacrine, the first cholinesterase inhibitor approved for AD treatment, did not stop the progression of AD, producing only some cognitive improvements, but exhibited secondary effects mainly due to its hepatotoxicity. Thus, the drug was withdrawn from the clinics administration. Since then, many publications have described non-hepatotoxic tacrines, and in addition, important efforts have been made to design multitarget tacrines by combining their cholinesterase inhibition profile with the modulation of other biological targets involved in AD.

Ecophysiological studies have highlighted the relevance of the avian immune system in individual fitness prospects in the wild. However, studies on the ontogeny of avian immunity are scarce. We analyse age-related changes in the cellular constitutive immunity throughout nestling development, as well as its relationship with sex and brood size. We found that cellular constitutive immunity could be affected by age, sex, brood size, or daily rhythm. Early-stage nestlings relied more on cells of the innate immunity rather than on cells linked to the adaptive immune system. Cellular immunity may not be fully mature in fledglings, as reflected by differences in phagocytic cell counts with regard to adults. Beyond the age-dependent effects, agranulocyte cell counts were affected by sibling competition while granulocyte cell counts showed a daily rhythm. We also show that the heterophil to lymphocyte ratio was negatively related to body weight when nestlings become more independent. Our study contributes knowledge to the fields of developmental immunology and ecological immunology based on essential components of the cellular immune system.

[EN] The accuracy of the predictions of age-specific probabilities of death is an essential objective for the insurance industry since it dramatically affects the proper valuation of their products. Currently, it is crucial to be able to accurately calculate the age-specific probabilities of death over time since insurance companies' profits and the social security of citizens depend on human survival; therefore, forecasting dynamic life tables could have significant economic and social implications. Quantitative tools such as resampling methods are required to assess the current and future states of mortality behavior. The insurance companies that manage these life tables are attempting to establish models for evaluating the risk of insurance products to develop a proactive approach instead of using traditional reactive schemes. The main objective of this paper is to compare three mortality models to predict dynamic life tables. By using the real data of European countries from the Human Mortality Database, this study has identified the best model in terms of the prediction ability for each sex and each European country. A comparison that uses cobweb graphs leads us to the conclusion that the best model is, in general, the Lee-Carter model. Additionally, we propose a procedure that can be applied to a life table database that allows us to choose the most appropriate model for any geographical area.

The service-learning methodology combines active learning processes and community service. This service-learning experience was performed using an interdisciplinary and cross plan. The teachers made a horizontal coordination in the courses, and a vertical coordination in subjects of the Degrees involved. This allowed working together in the students’ curricular training process. It also permitted covering various specific skills, as corresponds to the different subjects, whilst optimizing the students’ workload. The service addressed the problem of drug-facilitated sexual assaults (DFSA) in the youth leisure nightlife. DFSA is the temporary disability of a person caused by a decrease in her/his volitional and cognitive abilities due to the voluntary or involuntary consumption of a psychoactive substance. An active learning about the problem was encouraged in the classroom, focused on recognizing myths, attitudes, and risk situations. The service-learning actions to the community was based on an anonymous survey conducted among the students, which dealt with the problem. The Service Learning was stimulated through the design, planning and development of activities aimed at gaining social awareness of the existing problem while favouring peer learning processes. The students undertook awareness actions at different levels, spreading their message by means of social networks, high school workshops, and information stands on the street.

In distributed optical fibre sensors, the measurement range is typically limited to a few tens of kilometers due to the fibre attenuation and the input pump power limits imposed by the emergence of nonlinear effects. To overcome these problems, distributed amplification schemes have been investigated, generally employing continuous-wave pumping schemes. In this article, we propose the use of Raman amplification with an engineered intensity modulation. We show that this kind of assistance may provide, in principle, a close to perfect loss compensation along the fibre length. To find the optimal modulation profile, an analytical model is presented, and numerically, its performance is analyzed. Using a close to optimal pump modulation profile, we experimentally demonstrate a flat optical trace along 50 km of fibre with a minimal SNR penalty along the length. The result is favorably compared with the continuous-wave pumping case injecting the same average power. Thanks to this new approach, the sensitivity in the worst point is considerably better and the ASD noise floor is also reduced.

[Aim]: Historical climate variations, current climate and human impacts are known to influence current species richness, but their effects on phylogenetic and trait diversity have been seldom studied. We investigated the relationship of these three factors with the independent variations of species, phylogenetic and trait diversity of European mammals. Considering the position of the 0 ºC isotherm in the Last Glacial Maximum as a tipping point, we tested the following hypotheses: northern European assemblages host fewer species than southern European ones; northern areas harbour trait and phylogenetically clustered assemblages, while the more stable southern areas host random or overdispersed assemblages; and species richness correlates positively with human influence, while phylogenetic and trait diversity show clustered patterns in areas with stronger human influence., [Location]: Western Palaearctic., [Time period]: Current and Late Pleistocene effects on present-day diversity., [Major taxa studied]: Terrestrial mammals., [Methods]: We used a novel analytical approach based on distance matrices to separate the independent variations of species, phylogenetic and trait diversity, and assessed their relationships with current climate, climate stability and human influence through structural equation models., [Results]: The species-poor assemblages from northern Europe show higher phylogenetic and trait clustering than the more stable richer southern areas. However, no assemblage presented trait or phylogenetic overdispersion. Current climate is the primary driver of phylogenetic and trait diversity, while species richness is affected similarly by both current and past climates. Higher human influence correlates positively with species richness and trait diversity, both directly and by mediating indirect effects of present climate. Main conclusions: Current climate, climate stability and human influence affect the studied aspects of diversity, although the form and magnitude of their effects vary through space. Importantly, higher levels of human disturbances correlate with more species rich and trait diverse assemblages, an apparently counterintuitive result that deserves further study.

According to the competitive exclusion principle, species with low competitive abilities should be excluded by more efficient competitors; yet, they generally remain as rare species. Here, we describe the positive and negative spatial association networks of 326 disparate assemblages, showing a general organization pattern that simultaneously supports the primacy of competition and the persistence of rare species. Abundant species monopolize negative associations in about 90% of the assemblages. On the other hand, rare species are mostly involved in positive associations, forming small network modules. Simulations suggest that positive interactions among rare species and microhabitat preferences are the most probable mechanisms underpinning this pattern and rare species persistence. The consistent results across taxa and geography suggest a general explanation for the maintenance of biodiversity in competitive environments.

We present, for the first time, an experimental demonstration of RIN noise transfer dampening at low frequencies in random distributed feedback ultralong Raman fibre lasers based on conventional telecommunication fibres. Furthermore, we present a thorough theoretical description of the phenomenon and demonstrate how our model can be used to predict the observed behaviour, identifying the general requirements for system improvement through RIN transfer reduction.

Dual frequency comb (DFC) spectroscopy using electro-optic comb generators stands out for its flexibility, easy implementation, and low cost. Typically, two combs with different line spacing are generated from a common laser using independent electro-optic comb generators. This approach minimizes the impact of laser phase noise; however, the distinct paths followed by the two combs ultimately limit the attainable signal-to-noise ratio and long-term stability of the system. In this work, a common-path DFC is generated using a single modulator driven by an arbitrary waveform generator, thus enabling a remarkable increase of the system stability (up to 0.8 s of integration time) while maintaining high flexibility. The proposed technique is experimentally validated by implementing a dual frequency comb with 3000 lines, covering an optical bandwidth of 4.5 GHz, and demonstrating an optical-to-radiofrequency compression factor of 7500. Our system is able to measure extremely narrowband optical features (in the MHz range) with an accuracy only limited by the master laser stability.

Poly(N-isopropylacrylamide-co-methacrylic acid) hydrogels were structured with hydrophobic compartments. To achieve that, N-isopropylacrylamide was copolymerized with methacrylic acid and a small percentage (~0.4 %mol) of (3,4-dihydroxy-5-nitrophenyl)methacrylamide (nitrocatechol monomer) in the presence of sodium dodecyl sulphate at a concentration below its CMC value. All the results support the structuring of poly(N-isopropylacrylamide-co-methacrylic acid) hydrogels with hydrophobic domains in water. These compartments were found to be rich in methacrylic acid and nitrocatechol units. Moreover, the structuring disappeared when hydrogels were swollen at pH 7.4. The structured hydrogel was capable of loading hydrophobic molecules (methyl-2-naphthoate and methyl-9-anthracenecarboxylate) as well as charged drugs (doxorubicin hydrochloride). This hydrogel permitted cell adhesion and growth as well as its detachment when the temperature fell below the LCST.

Continuous, real-time monitoring of surface seismic activity around the globe is of great interest for acquiring new insight into global tomography analyses and for recognition of seismic patterns leading to potentially hazardous situations. The already-existing telecommunication fiber optic network arises as an ideal solution for this application, owing to its ubiquity and the capacity of optical fibers to perform distributed, highly sensitive monitoring of vibrations at relatively low cost (ultra-high density of point sensors available with minimal deployment of new equipment). This perspective article discusses early approaches on the application of fiber-optic distributed acoustic sensors (DASs) for seismic activity monitoring. The benefits and potential impact of DAS technology in these kinds of applications are here illustrated with new experimental results on teleseism monitoring based on a specific approach: the so-called chirped-pulse DAS. This technology offers promising prospects for the field of seismic tomography due to its appealing properties in terms of simplicity, consistent sensitivity across sensing channels, and robustness. Furthermore, we also report on several signal processing techniques readily applicable to chirped-pulse DAS recordings for extracting relevant seismic information from ambient acoustic noise. The outcome presented here may serve as a foundation for a novel conception for ubiquitous seismic monitoring with minimal investment.

Ecophysiological studies have highlighted the relevance of the avian immune system in individual fitness prospects in the wild. However, studies on the ontogeny of avian immunity are scarce. We analyse age-related changes in the cellular constitutive immunity throughout nestling development, as well as its relationship with sex and brood size. We found that cellular constitutive immunity could be affected by age, sex, brood size or daily rhythm. Early-stage nestlings relied more on cells of the innate immunity rather than on cells linked to the adaptive immune system. Cellular immunity may not be fully mature in fledglings, as reflected by differences in phagocytic cell counts with regard to adults. Beyond the age-dependent effects, agranulocyte cell counts were affected by sibling competition while granulocyte cell counts showed a daily rhythm. We also show that the heterophil to lymphocyte ratio was negatively related to body weight when nestlings become more independent. Our study contributes knowledge to the fields of developmental immunology and ecological immunology based on essential components of the cellular immune system.