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O presente artigo teve como objetivo analisar como o pensamento integrado foi evidenciado nos relatos integrados das estatais estaduais brasileiras, no período de 2015 a 2019. A amostra foi composta por 20 empresas controladas pelos estados brasileiros, totalizando 42 relatos integrados (RI). Para a coleta de evidências, fez-se uso da análise de conteúdo e o procedimento metodológico adotado foi a análise documental dos RI do período especificado para a pesquisa. Utilizou-se o Índice de Pensamento Integrado (IPI) composto por cinco dimensões, sendo analisados os elementos que necessitavam ser incorporados aos relatórios de RI bem como a correlação entre tais elementos. Os dados foram analisados de forma descritiva e por meio da correlação de Spearman. Os resultados indicaram que o RI no setor público ainda está em fase inicial de divulgação, bem como foi observada uma baixa frequência das variáveis que evidenciaram o pensamento integrado no conteúdo dos relatos integrados pesquisados. A maior evidenciação foi da dimensão Governança Eficaz, que demonstrou um forte compromisso com questões éticas e de sustentabilidade. Por outro lado, o menor índice encontrado foi na dimensão Conectividade. Nas análises da correlação, o foco externo indicou uma forte correlação com a governança eficaz e planejamento integrado, e uma fraca correlação com a comunicação integrada. A governança eficaz, por sua vez, apresentou uma forte correlação com o planejamento integrado. Os achados apontaram para a necessidade de maximizar o volume de informações acerca do pensamento integrado nos RIs. Palavras-chave: Relato Integrado. Pensamento Integrado. Estatais Estaduais Brasileiras. ABSTRACT This article aimed to analyze how integrated thinking was evidenced in the integrated reports of Brazilian state-owned companies, from 2015 to 2019. The sample consisted of 20 companies controlled by Brazilian states, totaling 42 integrated reports (IR). For the collection of evidence, content analysis was used and the methodological procedure adopted was the document analysis of the IR of the period specified for the research. The Integrated Thinking Index (ITI) composed of five dimensions was used, analyzing the elements that needed to be incorporated into the IR reports, as well as the correlation between such elements. Data were analyzed descriptively and using Spearman's correlation. The results indicated that IR in the public sector is still in the initial phase of dissemination, as well as a low frequency of variables that evidenced integrated thinking in the content of the integrated reports researched. The greatest disclosure was in the Effective Governance dimension, which demonstrated a strong commitment to ethical and sustainability issues. On the other hand, the lowest index found was in the Connectivity dimension. In correlation analyses, external focus indicated a strong correlation with effective governance and integrated planning, and a weak correlation with integrated communication. Effective governance, in turn, showed a strong correlation with integrated planning. The findings pointed to the need to maximize the volume of information about integrated thinking in IRs. Keywords: Integrated Reporting. Integrated Thinking. Brazilian State Companies.

O controle das finanças públicas se constitui como elemento essencial para a eficiência, eficácia e efetividade da gestão pública. O equilíbrio entre o que se arrecada e o que se gasta é primordial na Administração Pública, é uma informação relevante para que a sociedade conheça quanto está sendo arrecadado e destinado a áreas que afetam diretamente o dia a dia dos cidadãos. O objetivo geral do estudo é analisar a eficiência orçamentária, através do resultado fiscal e endividamento público. Do ponto de vista de sua natureza, a pesquisa se classifica como aplicada. No que se refere à abordagem do problema, foi utilizado o método quantitativo, com propósito exploratório. A técnica empregada para a coleta dos dados foi a pesquisa documental. Foi realizada uma pesquisa documental. Fizeram parte da amostra, todos os municípios do interior da Região Nordeste, que não são capitais, com população entre 300.000 e 700.000 habitantes. Percebeu-se que dados os municípios que fizeram parte dessa amostra possuem endividamento, contudo, Campina Grande, Mossoró e Camaçari são as cidades que apresentam o maior endividamento, com destaque para Campina Grande, que apresenta resultados superiores a 70%. Os melhores resultados foram observados nos municípios de Caucaia, Paulista e Feira de Santana, respectivamente. Palavras-chave: Contabilidade Pública. Eficiência de Gastos. Resultado Fiscal. Endividamento. ABSTRACT The control of public finances is an essential element for the efficiency, effectiveness and effectiveness of public management. The balance between what is collected and what is spent is essential in Public Administration, it is relevant information for society to know how much is being collected and destined to areas that directly affect the daily lives of citizens. The general objective of the study is to analyze the budget efficiency, through the fiscal result and public indebtedness. From the point of view of its nature, the research is classified as applied. Regarding the approach to the problem, the quantitative method was used, with an exploratory purpose. The technique used for data collection was documental research. A documentary research was carried out. All municipalities in the interior of the Northeast Region, which are not capital cities, with populations between 300,000 and 700,000 inhabitants were part of the sample. It was noticed that given the municipalities that were part of this sample, they have indebtedness, however, Campina Grande, Mossoró and Camaçari are the cities that have the highest indebtedness, especially Campina Grande, which presents results above 70%. The best results were observed in the municipalities of Caucaia, Paulista and Feira de Santana, respectively. Keywords: Public Accounting. Spending Efficiency. Tax Result. Indebtedness.

O desenvolvimento de competências técnicas e habilidades que o profissional contábil deve desenvolver até o final da graduação são pautas presentes nas diretrizes emitidas pela International Federation of Accountants (IFAC), inscritas na International Education Standard 2 e na International Education Standard 3, respectivamente. Nesta direção, este estudo busca analisar a percepção de alunos de Ciências Contábeis no que tange ao desenvolvimento das competências técnicas e habilidades profissionais definidas por estas diretrizes. Para tal, foi realizada uma pesquisa de caráter quantitativo a partir da aplicação de questionário junto a 289 alunos, com uso de estatística descritiva e aplicação de testes de diferenças entre grupos. Os resultados apontam que as competências técnicas que mais apresentam concordância entre os alunos de graduação estão relacionadas com as tomadas de decisões, situação atual e futura da entidade e controles e uso da tecnologia da informação como ferramenta para a tomada de decisões. Com relação às habilidades profissionais, identifica-se que há maior concordância com o desenvolvimento de habilidades gerais, como organização e liderança dentro das organizações. Indica-se ainda que o período cursado pelos alunos seja o agrupamento que apresenta maior número de diferenças significantes entre grupos. Posto isso, espera-se que haja maior fomento por parte das instituições de ensino frente ao desenvolvimento das competências técnicas e habilidades profissionais à luz da IFAC no curso de graduação, pois esse alinhamento entre os currículos pedagógicos e a IFAC podem garantir ganhos mercadológicos e educacionais para o campo disciplinar de Ciências Contábeis.

A pandemia da Covid-19 impactou fortemente o cenário jornalístico em relação a rotinas e, também, parte da maneira pela qual a população entende a atividade noticiosa, cuja credibilidade foi questionada constantemente nos últimos tempos. Em meio à pior emergência sanitária em um século, o jornalismo local-regional se afirma como uma das principais fontes de informação de uma sociedade em isolamento social. Este artigo tem como objetivo entender o agendamento da evolução e o espaço ocupado pela Covid-19 em dois dos principais sites jornalísticos do município de Ponta Grossa (PR): Diário dos Campos e ARede. Foram analisadas matérias publicadas ao longo do mês de março de 2020. A análise dos resultados indica formas de compreender o agendamento da enfermidade nos sites, representando uma dinâmica que pode ser considerada glocal. Palavras-Chave: Pandemia. Jornalismo Local-regional. Portais jornalísticos de Ponta Grossa. ABSTRACT The Covid-19 pandemic strongly impacted the journalistic scenario in relation to routines and, also, part of the way in which the population understands the news activity, whose credibility has been questioned constantly recently. In the midst of the worst health emergency in a century, local-regional journalism asserts itself as one of the main sources of information for a society in social isolation. This article aims to understand the evolution schedule and the space occupied by Covid-19 in two of the main journalistic sites in the municipality of Ponta Grossa (PR): Diário dos Campos and ARede. News articles published during March 2020 were analyzed. The analysis of the results indicates ways to understand the agenda-setting of the disease on the websites, representing a dynamic that can be considered glocal. Keywords: Pandemic. Local-regional Journalism. Ponta Grossa Journalistic Portals.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-09-30T22:34:02Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Viridiana Alves de Lara Silva.pdf: 1659364 bytes, checksum: 5f87f10a9f0e42a199b6ba99c8bded5e (MD5) Made available in DSpace on 2022-09-30T22:34:02Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Viridiana Alves de Lara Silva.pdf: 1659364 bytes, checksum: 5f87f10a9f0e42a199b6ba99c8bded5e (MD5) Previous issue date: 2022-07-27 A presente pesquisa objetiva analisar a política e programas de Estágio Curricular Supervisionado (ECS) de seis cursos de licenciaturas da UEPG, voltado para o Ensino Fundamental, nos contextos de ensino presencial e remoto, a partir da avaliação por/para uma agenda social, realizada pelos professores formadores. Parte-se do pressuposto que uma avaliação do ECS nas licenciaturas poderá contribuir para a compreensão da complexidade que envolve a formação docente e os processos formativos teórico-práticos desenvolvidos durante sua realização no curso de Licenciatura. Os objetivos específicos foram assim traçados: a) realizar a revisão sistemática de literatura sobre a produção acadêmica brasileira, na forma de teses e dissertações, voltada à temática de ECS das Licenciaturas (Ensino Fundamental); b) caracterizar a concepção da política de ECS das licenciaturas da UEPG; c) identificar potencialidades, limites e desafios do ECS, no ensino presencial e no ensino remoto, evidenciados nos resultados do processo avaliativo realizado pelos professores formadores; e d) delinear, a partir da teoria da avaliação de políticas e programas educacionais, os principais aspectos, tendências, desafios do ECS no ensino presencial e no ensino remoto. A questão norteadora da pesquisa é a seguinte: Qual é a avaliação da política e programas de Estágio Curricular Supervisionado (Ensino Fundamental) das licenciaturas da UEPG de professores formadores que dele participam, nos contextos de ensino presencial e remoto? O referencial teórico baseou-se nas contribuições de Afonso (2009), Fernandes (2010, 2011, 2018), Bourdieu (2004a, 2004b, 2014), Mainardes e Stremel (2020), Pimenta (2012, 2019), entre outros. A pesquisa é de natureza qualitativa, do tipo estudo de caso avaliativo, e a análise documental, o questionário on-line e o grupo focal são os procedimentos adotados para a produção de dados. A Análise Textual Discursiva (ATD), proposta por Moraes e Galiazzi (2007), é a metodologia adotada para análise dos dados produzidos, os quais integram três categorias: 1) Avaliação dos aspectos gerais da disciplina de ECS; 2) Avaliação das experiências de ensino dos professores formadores no contexto presencial e remoto; e 3) Avaliação do papel formativo do ECS frente às necessidades de revitalizar a política de ECS nas licenciaturas da UEPG. As potencialidades, fragilidades e similaridades do ECS nos contextos presenciais e remoto emergiram na avaliação da política e dos programas das Licenciaturas, quando os professores formadores apontam os pontos contundentes do ECS no presencial e as dificuldades e fragilidades vivenciadas durante o ensino remoto, sinalizando a construção de novos habitus no desenvolvimento da disciplina. Além disso, ressaltaram a relevância de discussões coletivas que foram realizadas na instituição para recontextualização da política no contexto da prática no período de isolamento social provocado pela pandemia da Covid-19. A avaliação das políticas e programas por/para uma agenda social caracterizada pelo raciocínio indutivo, pela aceitação de múltiplas realidades e avaliadores, pela diversidade de dados coletados, pela descrição, considerando as perspectivas dos professores formadores e suas assumidas preocupações sociais, éticas, políticas e pedagógicas desveladas nesta tese, pode contribuir para a compreensão do campo de investigação do ECS, bem como para o campo de estudos da Avaliação de Políticas e Programas Educacionais. The present research aims to analyze the Supervised Curricular Internship (SCI) policy and programs of six licentiateship courses at UEPG, focused on Primary Education, in face-to-face and distance learning contexts, based on the evaluation carried out by the teacher educators. It is assumed that an evaluation of the SCI in licentiateship courses may contribute to the understanding of the complexity involved in teacher education and the theoretical-practical training processes developed during its completion in the licentiateship course. The specific objectives were outlined as follows: a) to carry out a systematic literature review on the Brazilian academic production, in the form of theses and dissertations, focused on the theme of SCI of licenciateship courses (Primary Education); b) to characterize the conception of the SCI policy of the UEPG's licentiateship courses; c) to identify potentialities, limits, and challenges of the SCI, both in face-to-face and distance learning, evidenced in the results of the evaluation process carried out by the teacher educators; and d) to outline, based on the theory of evaluation of educational policies and programs, the main aspects, trends, and challenges of the SCI in face-to-face and distance learning. The guiding question of the research is the following: What is the evaluation of the policy and programs of the Curricular Supervised Internship (Primary Education) of UEPG's licentiateship courses of teacher educators who participate in it, in faceto-face and distance learning contexts? The theoretical framework was based on the contributions of Afonso (2009), Fernandes (2010, 2011, 2018), Bourdieu (2004a, 2004b, 2014), Mainardes and Stremel (2020), Pimenta (2012, 2019), among others. The research is qualitative in nature, of the evaluative case study type, and document analysis. The online questionnaire and the focus group are the procedures adopted for data production. The Textual Discourse Analysis (TDA), proposed by Moraes and Galiazzi (2007), is the methodology adopted for the analysis of the data produced, which comprise three categories: 1) Evaluation of the general aspects of the SCI subject; 2) Evaluation of the teaching experiences of teacher educators in face-to-face and distance contexts; and 3) Evaluation of the formative role of the SCI in relation to the need to revitalize the SCI policy in licentiateship courses at UEPG. The potentialities, weaknesses and similarities of the SCI in face-to-face and distance contexts emerged in the evaluation of the licentiateship policies and programs, when the teacher educators point out the strong points of the SCI in face-to-face learning and the difficulties and weaknesses experienced during distance learning, indicating the construction of new habitus in the development of the subject. Moreover, they emphasized the relevance of collective discussions that were held in the institution to recontextualize the policy in the context of practice in the period of social isolation caused by the Covid-19 pandemic. The evaluation of policies and programs by/for a social agenda characterized by inductive reasoning, acceptance of multiple realities and evaluators, diversity of data collected, description, considering the perspectives of teacher educators and their assumed social, ethical, political, and pedagogical concerns unveiled in this thesis, can contribute to the understanding of the SCI research field, as well as to the field of studies of the Evaluation of Educational Policies and Programs.

Made available in DSpace on 2021-07-14T10:46:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-10. Added 1 bitstream(s) on 2021-07-14T11:37:50Z : No. of bitstreams: 1 S1413-70542008000500024.pdf: 93943 bytes, checksum: 3d864179b7b4537e55a0821c40982295 (MD5) O cultivo protegido, entre outras vantagens, possibilita diminuir a sazonalidade na oferta e melhorar a qualidade das hortaliças. No entanto, constitui-se em tecnologia de elevado custo, que pode ser atenuado com o aumento na eficiência do uso da área protegida, proporcionado pelo cultivo consorciado. O trabalho foi realizado em casa de vegetação, na Unesp, Jaboticabal, SP, no período de agosto e novembro de 2005, e objetivou-se verificar o custo operacional total (COT) e a rentabilidade líquida da alface crespa 'Verônica' (Lactuca sativa L.) em monocultura e consorciada com pepino japonês 'Hokushin' (Cucumis sativus L.), em ambiente protegido. Os principais itens de custos estimados foram os referentes à operações, insumos e materiais. Para a alface crespa em monocultura e em consórcio, o COT foi de R$ 696,37 e R$ 295,06/614,4 m², e as produtividades de 890,78 e 822,53 kg/614,4 m², respectivamente em monocultura e em consórcio e as receitas brutas obtidas foram de R$ 489,93 e R$ 452,39/614,4 m². Em cultivo protegido, a monocultura de alface teve prejuízo de R$206,44/614,4 m², enquanto, em consórcio com pepino, a cultura da alface crespa 'Verônica' teve lucro operacional de R$ 157,33/614,4 m². The protected culture, among other advantages, made possible to diminish the seasoning in offers and to improve the quality of vegetables. However, one consists in technology of high cost, that it may be attenuated with the increase in the efficiency of the use of the protected area, proportionated for the intercropping. The experiment was carried out at greenhouse at UNESP, Jaboticabal-SP, in the period of August and November of 2005, with the aim of evaluating the operational cost (COT) and the profitability of the crisphead lettuce Verônica (Lactuca sativa L.) in monoculture and in intercropping with the Japanese cucumber Hokushin (Cucumis sativus L.), in protected environment. The main cost items analyzed were the operational cost, the agricultural amendments and the materials cost. For the crisphead lettuce in monoculture and in intercropping, the COT was R$ 696,37 and R$ 295,06/614,4 m² respectively and the productivity was 822.53 and 890.78 kg/614,4 m². In monoculture and in intercropping the gross incomes were R$ 489,93 and R$ 452,39/614,4 m² respectively. It was verified a loss of R$ 206,44/614,4 m² in the crisphead lettuce monoculture and an operational profit of R$ 157,33/614,4 m² in protected environment when in intercropping with cucumber. Universidade Estadual Paulista, Departamento de Produção Vegetal/PV Universidade Estadual Paulista, Departamento de Economia Rural/ER Universidade Federal do Semi-Árido/UFERSA, Departamento de Ciências Vegetais/CV Universidade Estadual de Ponta Grossa/UEPG, Departamento de Fitotecnia e Fitossanidade/DEFITO Universidade Estadual Paulista, Departamento de Produção Vegetal/PV Universidade Estadual Paulista, Departamento de Economia Rural/ER

Made available in DSpace on 2022-05-01T09:47:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Universidade Federal do Paraná Fundação Araucária Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Accidents involving Brown spiders are reported throughout the world. In the venom, the major toxins involved in the deleterious effects are phospholipases D (PLDs). In this work, recombinant mutated phospholipases D from three endemic species medically relevant in South America (Loxosceles intermedia, L. laeta and L. gaucho) were tested as antigens in a vaccination protocol. In such isoforms, key amino acid residues involved in catalysis, magnesium-ion coordination, and binding to substrates were replaced by Alanine (H12A-H47A, E32A-D34A and W230A). These mutations eliminated the phospholipase activity and reduced the generation of skin necrosis and edema to residual levels. Molecular modeling of mutated isoforms indicated that the three-dimensional structures, topologies, and surface charges did not undergo significant changes. Mutated isoforms were recognized by sera against the crude venoms. Vaccination protocols in rabbits using mutated isoforms generated a serum that recognized the native PLDs of crude venoms and neutralized dermonecrosis and edema induced by L. intermedia venom. Vaccination of mice prevented the lethal effects of L. intermedia crude venom. Furthermore, vaccination of rabbits prevented the cutaneous lesion triggered by the three venoms. These results indicate a great potential for mutated recombinant PLDs to be employed as antigens in developing protective vaccines for Loxoscelism. Department of Cell Biology Federal University of Paraná (UFPR) Production and Research Center of Immunobiological Products (CPPI) State Department of Health Veterinary Hospital Federal University of Paraná (UFPR) Department of Structural Molecular Biology and Genetics State University of Ponta Grossa (UEPG) Multiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP) Multiuser Center for Biomolecular Innovation Departament of Physics Universidade Estadual Paulista (UNESP) Universidade Federal do Paraná: 02/2020 Universidade Federal do Paraná: 04/2019 Fundação Araucária: 057/2017 CNPq: 303868/2016-3 CNPq: 408633/2018-2

Made available in DSpace on 2022-04-29T08:38:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 B chromosomes are additional dispensable elements to the standard chromosomal set of an organism. In most cases, their transmission differs from Mendelian patterns, leading to their accumulation or extinction. The present study aimed to describe, for the first time, the transmission pattern of B chromosome in a population of Psalidodon paranae through directed crosses, as well as to analyze the populational dynamics of B chromosome. Our results revealed the possible elimination of B chromosome in crossings where only females were B-carriers, with a mean transmission rate (kB) of 0.149; however, kB was significantly higher in crossings involving male B-carriers (kB = 0.328-0.450). Moreover, we observed an increase in the frequency of B chromosomes in the natural population of P. paranae in the last two decades. These apparently contradictory results can make sense if the B chromosome provides adaptive advantages to their carriers. Here, we observed a differential transmission of B chromosomes in each sex of parental individuals, with higher transmission rates in crossing involving males B-carriers, in addition to describe the temporal changes of B chromosome frequency in P. paranae. Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista (UNESP) júlio de Mesquita Filho Departamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista júlio de Mesquita Filho (UNESP) Departamento de Genética Instituto de Ciências Biológicas e da Saúde Icbs Universidade Federal Rural Do Rio de Janeiro Centro Nacional de Pesquisa e Conservacao da Biota Aquatica Continental (CEPTA-ICMBIO) Centro de Aquicultura da Unesp Universidade Estadual Paulista júlio de Mesquita Filho (UNESP) Departamento de Biologia Estrutural Molecular e Genética Setor de Ciências Biológicas e da Saúde Universidade Estadual de Ponta Grossa Departamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista (UNESP) júlio de Mesquita Filho Departamento de Biologia Estrutural e Funcional Instituto de Biociências Universidade Estadual Paulista júlio de Mesquita Filho (UNESP) Centro de Aquicultura da Unesp Universidade Estadual Paulista júlio de Mesquita Filho (UNESP)

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-10-03T21:01:12Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Priscila Salles de Brito.pdf: 4084653 bytes, checksum: 7b5d5bcd7e74c8d5bc807334ab2a4e94 (MD5) Made available in DSpace on 2022-10-03T21:01:12Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Priscila Salles de Brito.pdf: 4084653 bytes, checksum: 7b5d5bcd7e74c8d5bc807334ab2a4e94 (MD5) Previous issue date: 2022-08-26 Este trabalho tem por objetivo investigar a morfoanatomia, composição química e atividades biológicas do óleo essencial de Eucalyptus tereticornis. Para tanto foi avaliada a morfoanatomia das folhas adultas de E. tereticornis, e após a extração do óleo essencial das folhas de E. tereticornis foi determinado o seu teor e o perfil químico e verificadas algumas atividades biológicas como, atividades antimicrobiana, antioxidante, efeito anti-inflamatório por meio do modelo de edema de orelha, migração celular por meio de análises histológicas, atividade citotóxica e a ação repelente. No presente estudo, uma explicação abrangente da anatomia da folha e do caule e histoquímica de Eucalyptus tereticornis é apresentada. Observou-se a presença de crostas como ceras epicuticulares nas superfícies das folhas, os cristais foram encontrados em algumas células epidérmicas como um agregado de placas, presença de cristais prismáticos em vários tecidos e mesofilo homogêneo. As análises histoquímicas confirmaram a presença de compostos lipofílicos e compostos fenólicos, grãos de amido e elementos lignificados. A composição química do óleo essencial análise por CGEM detectou 25 compostos, sendo que os principais componentes foram p-cimeno (46,57%), a criptona (25,24%) e o terpinen-4-ol (5,23%). Na análise da atividade antimicrobiana, houve inibição do crescimento de todos os microrganismos testados na metodologia de difusão em disco, com resultados mais satisfatórios frente a C. albicans e S. epidermidis; na metodologia de macrodiluição em caldo, com exceção de P. aeruginosa, também foi observada atividade bactericida do óleo essencial frente todas as demais cepas testadas. O óleo essencial de E. tereticornis demonstrou efeito antioxidante pelo método da redução do radical livre DPPH. O óleo essencial de E. tereticornis apresentou atividade anti-inflamatória quando utilizado por via tópica, pois reduziu de forma significativa o edema, o peso do tecido e a migração de células inflamatórias. Na avaliação da atividade citotóxica, por meio de redução do MTT utilizando as linhagens de fibroblasto murino (linha não tumoral - 3T3) e de melanoma murinho (linhagem tumoral - B16F10), observou-se que é necessário utilizar uma concentração maior do óleo para causar citotoxicidade para a linhagem tumoral em relação a não tumoral. Na avaliação da atividade repelente, também foi observado efeito com o óleo sem diluição. Esses resultados mostram detalhes da morfoanatomia e histologia da planta ainda não estudados. Novos estudos envolvendo análises anatômicas e filogenéticas de outras espécies de eucalipto são indispensáveis para entender melhor a evolução e taxonomia do gênero. O presente trabalho mostra também que a necessidade da continuidade nos estudos para confirmar e elucidar o mecanismo de ação dessas e muitas outras atividades biológicas que podem estar presentes no o óleo essencial de E. tereticornis. This study aims to investigate the morphoanatomy, chemical composition and biological activities of the essential oil of Eucalyptus tereticornis. Therefore, the morphoanatomy of the adult leaves of E. tereticornis was evaluated, and after the extraction of the essential oil from the leaves of E. tereticornis, its content and chemical profile were determined and some biological activities were verified, such as antimicrobial, antioxidant, anti-inflammatory activity. -inflammatory through the ear edema model, cell migration through histological analysis, cytotoxic activity and repellent action. In the present study, a comprehensive explanation of the leaf and stem anatomy and histochemistry of Eucalyptus tereticornis is presented. The presence of crusts such as epicuticular waxes on the leaf surfaces was observed, crystals were found in some epidermal cells as an aggregate of plaques, presence of prismatic crystals in various tissues and homogeneous mesophyll. Histochemical analyzes confirmed the presence of lipophilic and phenolic compounds, starch grains and lignified elements. The chemical composition of the essential oil by GC-MS analysis detected 25 compounds, the main components being p-cymene (46.57%), kryptone (25.24%) and terpinen-4-ol (5.23 %). In the analysis of antimicrobial activity, there was inhibition of the growth of all microorganisms tested in the disc diffusion methodology, with more satisfactory results against C. albicans and S. epidermidis; in the broth macrodilution methodology, with the exception of P. aeruginosa, bactericidal activity of the essential oil was also observed against all other strains tested. The essential oil of E. tereticornis showed an antioxidant effect by the DPPH free radical reduction method. E. tereticornis essential oil showed antiinflammatory activity when used topically, as it significantly reduced edema, tissue weight and migration of inflammatory cells. In the evaluation of the cytotoxic activity, through the reduction of MTT using the murine fibroblast (non-tumor line - 3T3) and murine melanoma (tumor line - B16F10) strains, it was observed that it is necessary to use a higher concentration of the oil to cause cytotoxicity for the tumor lineage in relation to the nontumor. In the evaluation of the repellent activity, an effect was also observed with the oil without dilution. These results show details of the morphoanatomy and histology of the plant not yet studied. New studies involving anatomical and phylogenetic analyzes of other eucalyptus species are essential to better understand the evolution and taxonomy of the genus. The present work also shows the need for continuity in studies to confirm and elucidate the mechanism of action of these and many other biological activities that may be present in the essential oil of E. tereticornis.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-09-26T21:25:54Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Jociane Dias Batista.pdf: 1536421 bytes, checksum: d4a2bd79f0f9e0802f7d68dc09443daa (MD5) Made available in DSpace on 2022-09-26T21:25:54Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Jociane Dias Batista.pdf: 1536421 bytes, checksum: d4a2bd79f0f9e0802f7d68dc09443daa (MD5) Previous issue date: 2022-07-08 A agricultura vem tendo grandes avanços tecnológicos em função da incorporação de novas tecnologias. Uma das consequências é a necessidade de tratar com grande volume de dados gerados. Na busca constante por informações confiáveis e atualizadas, em tempo real, há necessidade de estratégias mais eficientes e decisões mais assertivas, com intuito de maximizar rendimentos e reduzir insumos agrícolas e recursos naturais. Essa busca exige uma abordagem intensiva de dados, e coloca aspectos tecnológicos e dados no centro da atenção, examinando os processos, atores e relacionamentos em ambientes agrícolas. Porém, mesmo existindo uma grande disponibilidade de dados, estes dados são provenientes de diversas fontes e coletados em diferentes formatos. Consequentemente, para tratar com a heterogeneidade desses dados, existe a necessidade de criar soluções que reduzam essa disparidade entre os diferentes sistemas de informações que constam em cada etapa dos processos de produção agrícola. Nesse sentido, as ontologias são artefatos que permitem organizar o conhecimento dentro de um determinado domínio, o compartilhamento e o reuso de informações, bem como, a integração de informações e a interoperabilidade entre sistemas. Diante deste contexto, nesta pesquisa, o objetivo foi mapear as principais classes de dados geradas durante cada etapa dos processos envolvidos no sistema de cultivo da soja, por meio de uma ontologia de domínio, com propósito de contribuir com a análise e gestão dos dados, e recursos que facilitem a troca de informações entre agentes e sistemas que atuam nessas etapas. Para criação da ontologia foi utilizada metodologia Ontology Development 101 e a ferramenta para edição de ontologias Protégé. Como resultado é apresentado um modelo de conhecimento semântico proposto para representar as principais classes de dados mapeadas no sistema de cultivo da soja. Agriculture has been making great technological advances due to the incorporation of modern technologies. One of the consequences is the need to deal with a large volume of generated data. To constantly reduce the real efforts and the searches, in time, the most efficient strategies and the most efficient decisions, they optimize the yield and the agricultural inputs and the natural resources. This requires a data-intensive approach and puts technology and data aspects at the center of attention, examining processes, and relationships in business areas. However, there is a wide availability of sources and data in different formats. Consequently, to deal with the heterogeneity of these data, there is a need to create solutions that reduce this disparity between the different information systems that appear at each stage of the agricultural production processes. In this sense, ontologies are defined as information that allow knowledge within a given domain, the sharing and set of information, as well as the integration of information and interoperability between systems. Given this context, in this research, the objective was to map the main classes of data generated during each stage of the processes involved in the soybean cultivation system, through a domain ontology, with the purpose of contributing to the analysis and management of data, and resources that facilitate the exchange of information between agents and systems that work in these stages. To create the ontology, the Ontology Development 101 methodology and the ontology editing tool Protégé were used. As a result, a proposed semantic knowledge model is presented to represent the main classes of data mapped in the soybean cropping system.

Made available in DSpace on 2021-06-25T10:24:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Haemotropic mycoplasmas (haemoplasmas) are small pleomorphic bacteria infecting erythrocytes of several mammalian species, including human beings. No study to date has focused on the risk of bacteria exposure in hunting activities, particularly in natural environments of highly tick-infested areas. Accordingly, the present study aimed to assess haemoplasma occurrence in the complex encompassing wild boars, hunting dogs and hunters of Brazil. A total of 38/65 (58.5%) wild boars and 94/159 (59.1%) dogs were positive by qPCR for at least one haemoplasma. All 25 hunters were negative. Dogs with high hunting frequency were 2.4 more likely to be infected. Sequencing revealed a probable novel haemoplasma species in wild boars. Although exposure to haemoplasma species was present, the study herein found no evidence of cross-species transmission. Department of Comparative Pathobiology Purdue University Department of Cell and Molecular Biology Universidade Federal do Paraná Biotechnology Institute Universidade Estadual Paulista (UNESP) Department of Preventive Medicine Universidade de São Paulo Department of Medicine Universidade Estadual de Ponta Grossa Department of Veterinary Medicine Universidade Federal do Paraná Department of Parasitology Institute of Biological Sciences Universidade Federal de Minas Gerais Biotechnology Institute Universidade Estadual Paulista (UNESP)

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-09-20T17:17:46Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Vinícius Gabriel Ribicki.pdf: 1860383 bytes, checksum: 3d333ad0a0b76035cf90decb9384a140 (MD5) Made available in DSpace on 2022-09-20T17:17:47Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Vinícius Gabriel Ribicki.pdf: 1860383 bytes, checksum: 3d333ad0a0b76035cf90decb9384a140 (MD5) Previous issue date: 2022-06-15 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior O aumento na geração de efluentes demandam unidades compactas e eficientes afim de se atender aos padrões de lançamento. Os reatores biológicos de leito empacotado oferecem meio para suporte para biomassa ativa e formação de gradientes de oxigênio fomentando o crescimento simultâneo de bactérias nitrificantes e desnitrificantes para remoção de nitrogênio. A performance do reator não depende apenas da cinética microbiana e enzimática, mas também dos processos físicos envolvidos. O empacotamento promove um escoamento não uniforme, fomentando anomalias no sistema. O presente trabalho teve por objetivo a avaliação hidrodinâmica de um reator de leito empacotado, em distintas condições de operação. O reator cilíndrico de fluxo ascendente com volume útil de 10,08 L teve recheio de mídias Mini Biobob®, operado com tempos de detenção hidráulicas (TDH) de 8 h, 14 h e 20 h, razões de recirculação R0, R1 e R2, com/sem aeração. O regime do reator foi definido como próximo à mistura completa, sendo verificado aumento na mistura com a redução do TDH para a etapa não aerada e não observado sua influência para operação aerada. Os ensaios revelaram TDH médio menor que o teórico estipulado, atrelado ao surgimento de anomalias. Para ambas as operações não houve influência na mistura com a variação das razões de recirculação, não influindo na espessura da camada limite e na transferência de massa. Foi encontrado volume morto médio de 23,17% e 22,53% para as etapas aerada e não aerada respectivamente. Não foi encontrada correlação estatística significativa para o parâmetro em relação às variáveis estudadas. The effluent generation increase requires compact and efficient units to comply with quality discharge standards. The biological packed bed reactors offer support for biomass and the establishment of oxygen gradients to foment the simultaneous growth of nitrifiers and denitrifiers bacteria for nitrogen removal. The reactor performance does not only rely on microbial and enzymatic kinetics but also the physical process involved. The packed bed creates an uneven flow, fomenting anomalies in the system. The present study aimed to evaluate the hydrodynamic of a packed bed reactor in distinct operational conditions. The upflow cylindrical reactor with a volume of 10.08 L was filled with Mini Biobob® media and operated in hydraulic retention times (HRT) of 8 h, 14 h, and 20 h, with recirculation ratios R0, R1, and R2, with/without aeration. The flow pattern was defined as close to a complete mix, it was verified mixture increases with the HRT reduction for the non-aerated operation, and no influence was observed for the aerated period. The experiments revealed that the mean HRT was lower than the theoretical stipulated due to anomalies. The impact on the mixture was not noticed for both operations when varying the recirculation ratio, not influencing the boundary layer thickness and mass transfer. A mean dead volume of 23.17% and 22.53% were found for the aerated and non-aerated operations, respectively. A significant statistical correlation was not found for the studied variables' parameters.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-07-28T17:40:22Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Fernando Roberto dos Santos.pdf: 3332096 bytes, checksum: bdbd8e0703314bed66ffa86c1963f130 (MD5) Made available in DSpace on 2022-07-28T17:40:22Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Fernando Roberto dos Santos.pdf: 3332096 bytes, checksum: bdbd8e0703314bed66ffa86c1963f130 (MD5) Previous issue date: 2022-11-10 A escassez de água doce é uma grande preocupação devido às mudanças climáticas em algumas regiões do Brasil. As taxas de evaporação da água em reservatórios aumentam com o aumento das temperaturas, o que pode representar um grande desafio para a gestão dos recursos hídricos. A área de cobertura dos sistemas fotovoltaicos flutuantes reduz a quantidade de luz solar na superfície da água, reduzindo as taxas de evaporação da água. Em 2019 foi instalado um sistema fotovoltaico flutuante no reservatório Passaúna, localizado na região metropolitana de Curitiba, Estado do Paraná, sul do Brasil. O objetivo geral deste trabalho é estudar os efeitos do sistema fotovoltaico flutuante sobre a evaporação da água no reservatório do Passaúna. Com base nesse objetivo, o trabalho foi dividido em dois capítulos: no primeiro capítulo foi estimada a evaporação evitada pela instalação do sistema fotovoltaico flutuante no reservatório do Passaúna, considerando a área de cobertura existente, com capacidade de 130 kWp, e um aumento na área de abrangência correspondente a 1 MWp, 2.5 MWp e 5 MWp em capacidade de produção de energia. Diferentes métodos foram utilizados para estimar as taxas de evaporação por meio de dados meteorológicos locais, como temperatura do ar, umidade, radiação solar e velocidade do vento, cujos resultados foram comparados com medições diretas de evaporação obtidas na estação meteorológica mais próxima. O método FAO Penman-Monteith e Linacre (1977) apresentou melhores resultados para a região. Obteve-se uma eficiência de 60,20% na redução das taxas de evaporação e economia de água pelo layout do sistema fotovoltaico flutuante, que foi então utilizado para calcular os volumes de evaporação evitados, assumindo os aumentos de área de cobertura e sua relação com a capacidade de produção de energia, considerando o reservatório em sua capacidade total e no atual cenário de seca hídrica. No segundo capítulo, foram estimadas as taxas de evaporação para cenários climáticos futuros para os anos de 2040, 2060, 2080 e 2100 considerando os cenários de mudanças climáticas do Painel Intergovernamental sobre Mudanças Climáticas. O software PGECLIMA_R foi utilizado para simular a temperatura do ar, umidade e radiação solar; o vento foi obtido através de uma simulação de distribuição beta. Um aumento na evaporação de 31,88% em relação aos dados históricos atuais pode ser esperado para o final do século no pior cenário climático. Portanto, tecnologias como o sistema fotovoltaico flutuante podem representar uma importante alternativa na prevenção da evaporação hoje e no futuro, tornando-se uma ferramenta estratégica na gestão dos recursos hídricos. Freshwater scarcity is a significant concern due to climate change in some regions of Brazil. Water evaporation rates in reservoirs increase with increasing temperatures, which can represent a great challenge for managing water resources. The coverage area of floating photovoltaic systems reduces the amount of sunlight on the water surface, reducing the water evaporation rates. In 2019 a floating photovoltaic system was installed in the Passaúna reservoir, located in the metropolitan region of Curitiba, State of Paraná, southern Brazil. The general objective of this work is to study the effects of the floating photovoltaic system in the Passaúna reservoir on water evaporation. Based on this objective, the work was divided into two chapters: in the first chapter, the evaporation avoided by the installation of the floating photovoltaic system in the Passaúna reservoir was estimated, considering the existing coverage area, with a capacity of 130 kWp, and expansions of the system to correspond to energy production capacities of 1 MWp, 2.5 MWp and 5 MWp. Different methods were used to estimate the evaporation rates through local meteorological data such as air temperature, humidity, solar radiation, and wind speed, whose results were compared with direct evaporation measurements obtained at the nearest meteorological station. The FAO Penman-Monteith and Linacre method (1977) showed better results for the region. An efficiency of 60.20% in reducing evaporation rates and water-saving by the floating photovoltaic system layout was obtained, which was then used to calculate avoided evaporation volumes, assuming the increases in coverage area and its relationship to energy production capacity, considering the reservoir at full capacity and in the current scenario of water drought. In the second chapter, evaporation rates were estimated for future climate scenarios for the years 2040, 2060, 2080, and 2100 considering the climate change scenarios from the Intergovernmental Panel on Climate Change. PGECLIMA_R software was used to simulate air temperature, humidity, and solar radiation; the wind was obtained through a beta distribution simulation. An increase in evaporation of 31.88% compared to current historical data can be expected towards the end of the century in the worst climate scenario. Therefore, technologies such as the floating photovoltaic system can represent an essential alternative in preventing evaporation today and in the future, becoming a strategic tool in managing water resources.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-07-21T16:22:21Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Alison Diego Leajanski .pdf: 3202332 bytes, checksum: e056df624fa4376c66496ca9c4b03980 (MD5) Made available in DSpace on 2022-07-21T16:22:21Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Alison Diego Leajanski .pdf: 3202332 bytes, checksum: e056df624fa4376c66496ca9c4b03980 (MD5) Previous issue date: 2022-05-27 Umas das principais iniciativas de conservação da natureza são as áreas protegidas ou unidades de conservação, que possuem objetivos de conservação da biodiversidade, geodiversidade e dos recursos naturais. Essas áreas podem ser criadas pela iniciativa privada ou pelo poder público, em suas diferentes esferas governamentais. No Brasil, a principal política ambiental voltada às unidades de conservação é a Lei nº 9.985/2000, que instituiu o Sistema Nacional de Unidades de Conservação (SNUC). O SNUC estabelece critérios e normas para a criação, implementação e gestão das unidades. A partir disso, a presente pesquisa tem como objetivo geral analisar como as diferentes esferas governamentais realizam a gestão do Parque Nacional dos Campos Gerais, Parque Estadual do Guartelá e Parque Municipal Chácara Dantas na região dos Campos Gerais do Paraná. Os objetivos específicos referem-se a identificar as possibilidades e dificuldades para a gestão das áreas de estudo, verificar se a gestão de cada parque está em consonância com as políticas ambientais vigentes e comparar, a partir das questões da entrevista, os principais aspectos da gestão realizada em cada um dos parques. Em relação aos procedimentos metodológicos, utilizou-se de pesquisa documental e, para a coleta de dados, optou-se pela realização de entrevistas semiestruturadas com os gestores dos parques. Quanto ao método que norteou o estudo, optou-se pela utilização da abordagem sistêmica. Os resultados evidenciaram diferenças no que diz respeito aos três parques, com destaque para o contexto em que foram criados e estão inseridos, o contexto histórico, político e econômico, o nível de implementação e o tamanho das áreas. Além disso, alguns aspectos se repetem, como a pressão causada pelas espécies exóticas, ambos os gestores destacaram essa presença como um desafio no contexto das áreas. Também percebeu-se que os parques analisados não estão em consonância com o SNUC e apresentam dificuldades em seguir o que a lei estabelece. Os locais apresentam diferentes graus de implementação, evidenciando que o poder público, em suas três esferas, tratou os parques de maneiras distintas ao longo das décadas. Por fim, considera-se que as políticas ambientais são importantes por apresentarem um respaldo legal para as unidades de conservação e para a gestão. Porém, somente a existência das políticas ambientais não garante a conservação, é preciso que sejam seguidas e aplicadas. No contexto das unidades de conservação, é preciso que haja maior interesse e empenho do poder público, em suas diferentes esferas, dos gestores e de toda a sociedade, que pode atuar por meio da ocupação dos espaços de representatividade. No momento em que a natureza está cada vez mais pressionada pelas ações humanas, são relevantes estudos que busquem debruçar-se sobre a temática ambiental, apontando caminhos para melhorar a conservação da natureza. Protected areas or Conservation Units are one of the most important nature conservation initiatives, with the goal of conserving biodiversity, geodiversity, and natural resources. These areas can be created by either the private or public sector in their respective governmental spheres. Law No. 9,985/2000, which established the National System of Conservation Units (SNUC), is the main environmental policy aimed at conservation units in Brazil. The SNUC establishes criteria and standards for unit creation, implementation, and management. Based on the foregoing, the general objective of this research is to analyze how the various governmental spheres manage the Campos Gerais National Park, Guartelá State Park, and Chácara Dantas Municipal Park in the Campos Gerais region of Paraná. The specific objectives include identifying the possibilities and difficulties for managing the study areas, determining whether the management of each park is in accordance with current environmental policies, and comparing the main aspects of management held in each of the parks based on interview questions. In terms of methodology, documental research was employed, and semi-structured interviews with park managers were chosen for data collection. The systemic approach was chosen as the study's methodology. The findings revealed differences between the three parks, with an emphasis on the scope in which they were created and inserted, the historical, political, and economic context, the level of implementation, and the size of the areas. Furthermore, some aspects appear repeatedly, such as the pressure caused by exotic species, which all managers identified as a challenge for these areas. It was also found that the analyzed parks are not in accordance with the SNUC and have difficulty adhering to what the law establishes. The locations are in different stages of implementation, demonstrating how public power, in its three spheres, has treated the parks in different ways over the decades. Finally, environmental policies are perceived as important because they provide legal support for conservation units and their management. However, the mere existence of environmental policies does not ensure conservation; they must be followed and applied. In the context of conservation units, there needs to be greater interest and commitment from the government, in its different spheres, from managers and from society, which can act through the occupation of representative spaces. At a time when human actions are putting increasing strain on nature, studies that seek to address environmental issues are crucial for pointing out ways to improve nature conservation.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-07-15T22:31:02Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Aline Rafaela de Almeida.pdf: 5985228 bytes, checksum: e903032115c27253472d4ca793ed4001 (MD5) Made available in DSpace on 2022-07-15T22:31:02Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Aline Rafaela de Almeida.pdf: 5985228 bytes, checksum: e903032115c27253472d4ca793ed4001 (MD5) Previous issue date: 2022-04-22 A presente tese aborda o aproveitamento de um resíduo agroindustrial para ser aplicado no tratamento de água, visto que o Brasil é um dos maiores responsáveis pela geração de produtos agroindustriais, tendo nesse setor um dos principais segmentos de sua economia. O bagaço de cana-de-açúcar é um dos resíduos mais produzidos no país, alcançando 83% da produção mundial dessa matéria-prima, o que o torna um grande gerador desse resíduo agroindustrial. No período de 2021/2020, o Brasil contribuiu para produção de 169 milhões de toneladas. Como essa biomassa já apresentou eficiência em relação ao seu potencial de adsorção in natura, ativado física ou quimicamente, possibilita também ser aplicada para outros métodos de remoção de contaminantes de água como processos oxidativos avançados (POA). Neste trabalho foi realizada modificação do bagaço de cana-de-açúcar de duas formas: íons de Fe (III) e Nb2O5, e os materiais foram aplicados em processo foto-Fenton, Fotocatálise heterogênea e adsorção na remoção de corante preto reativo 5 (PR5). Os materiais modificados foram caracterizados por meio de análise de Difração de raios X (DRX), Espectroscopia vibracional de absorção na região do infravermelho com transformada de Fourier (FTIR), Espectroscopia raios X por energia dispersiva (EDS) acoplada à microscopia eletrônica de varredura (MEV-FEG), Potencial zeta (ζ), Análise de área superficial (BET) e Espectroscopia de reflectância difusa (DRS). As caracterizações possibilitaram observar que os metais foram imobilizados na superfície do bagaço de cana; e tiveram um papel fundamental na eficiência dos processos quando comparados ao bagaço de cana in natura. Além disso, foi avaliado o efeito da reutilização dos materiais em até quatro vezes. Os melhores resultados obtidos foram para o material com imersão de 12h na solução de íons de Fe(III) para processo foto-Fenton que expressaram uma diminuição de aproximadamente 100% da banda responsável pelo grupo cromóforo (598nm) e a banda característica do grupo aromático (310nm) apresentou uma redução de aproximadamente 90%, após 60 min, nos espectros. Por outro lado, o melhor rendimento para processo de Fotocatálise heterogênea utilizando Nb2O5 foi o material sem tratamento alcalino e presença de H2O2 que apresentou uma diminuição de quase 65% no grupo cromóforo e de aproximadamente 40% no grupo aromático, enquanto para a adsorção expressou remoção sempre abaixo de 50% de remoção do grupo cromóforo e praticamente não houve alteração do grupo aromático. Os resultados demonstraram que bagaço de cana-de-açúcar com metais imobilizados pode ser aplicado tanto como material bioadsorvente como suporte para processos fotocatalíticos em tratamento de efluentes. This thesis is about the use of an agro-industrial residue to be applied in water treatment, Brazil is one of the main responsible for the generation of agro-industrial products, having this sector one of the main segments of its economy. Sugarcane bagasse is one of the most produced residues in the country, reaching 83% of the world’s production of this raw material, which makes it a great generator of this agro- industrial residue. In the period 2021/2020, Brazil contributed to the production of 169 million tons. As this biomass has already shown efficiency in relation to its in nature adsorption potential, physically or chemically activated, it can also be applied to other methods of removing water contaminants such as advanced oxidative processes (AOP). In this work, sugarcane bagasse was modified in two ways: Fe(III) and Nb2O5 ions, and the materials were applied in a photo-Fenton process, heterogeneous photocatalysis, and adsorption to remove reactive black dye 5 (RB5). The modified materials were characterized by X-ray Diffraction (XRD) analysis, vibrational absorption spectroscopy in the infrared region with Fourier transform (FTIR), energy dispersive X-ray spectroscopy (EDS) coupled with scanning electron microscopy ( SEM-FEG), Zeta potential (ζ), Surface area analysis (BET) and Diffuse reflectance spectroscopy (DRS). The characterizations made it possible to observe that the metals were immobilized on the surface of the sugarcane bagasse; and played a fundamental role in the efficiency of the processes when compared to fresh sugarcane bagasse. In addition, the effect of reusing materials up to four times was evaluated. The best results were obtained for the material with a 12h immersion in the Fe(III) ion solution for the photo-Fenton process, which expressed a decrease of approximately 100% of the band responsible for the chromophore group (598nm) and the characteristic band of the aromatic group. (310nm) showed a reduction of approximately 90%, after 60 min, in the spectra. On the other hand, the best yield for the heterogeneous photocatalysis process using Nb2O5 was the material without alkaline treatment and presence of H2O2, which showed a decrease of almost 65% in the chromophore group and approximately 40% in the aromatic group, while for the adsorption it expressed removal always below 50% removal of the chromophore group and practically no change in the aromatic group. The results showed that sugarcane bagasse with immobilized metals can be applied both bioadsorbent material as a support for photocatalytic processes in effluent treatment.

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-04-29T12:38:13Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Rodrigo Simionato.pdf: 4317441 bytes, checksum: 9874e5fa0a66a3428d18473cb96522a5 (MD5) Made available in DSpace on 2022-04-29T12:38:13Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Rodrigo Simionato.pdf: 4317441 bytes, checksum: 9874e5fa0a66a3428d18473cb96522a5 (MD5) Previous issue date: 2022-04-08 O presente estudo teve por objetivo geral identificar a atuação das categorias de base dos clubes de futebol não formadores em relação à legislação protetiva no âmbito dos direitos do adolescente jogador de futebol. Partiu-se da hipótese básica de que há um gap (lacuna) entre a legislação protetiva no âmbito dos direitos do adolescente jogador de futebol e o respeito destes direitos pelos seus clubes de base não formadores. Trata-se de uma pesquisa aplicada, exploratória, com abordagem qualitativa. O corpus documental foi composto por 09 entrevistas semiestruturadas com sujeitos integrantes da rede proteção dos direitos da criança e do adolescente com competência para fiscalizar os direitos dos adolescentes jogadores de futebol das categorias de base dos clubes Sport Club Campo Mourão e Prudentópolis Futebol Clube, além de um membro integrante deste, e por documentos institucionais. Para a análise dos dados qualitativos, foi adotada a técnica de Análise de Conteúdo. Após a análise constatou-se que há percepção pela rede de proteção no que tange ao desrespeito ao rol de direitos desses adolescentes integrantes das categorias de base do futebol pelos seus clubes que perpassam o ordenamento jurídico protetivo violando desde suas garantias sócio fundamentais contempladas constitucionalmente (CRFB/1988), quanto suas normas infra constitucionais, ECA, Lei Pelé e CLT até nas normativas administrativas das entidades de gestão desportiva, como a CBF e a FPF, mas que essa violência velada e silenciosa estrutural vêm à tona quando ocorrem denúncias desses abusos, mas sobretudo aquelas de grande repercussão na mídia. The present research aimed to investigate the procedure of football youth categories of non-training in relation to the protective legislation in the scope of the adolescent football player rights. The investigation started from the hypothesis that there is a gap between the protective legislation about adolescent football player rights and the respect of these rights by their junior clubs of non-training. In order to study this situation, this is an applied, exploratory research with a qualitative approach. The documentary corpus was composed of 09 semi-structured interviews with members of the protection network for the rights of children and adolescentes. These interviewed have competence to inspect the adolescent football players rights from youth categories of both Sport Club Campo Mourão and Prudentópolis Futebol Clube. It was also interviewed an integral member of Prudentópolis Futebol Clube, and the analysis was composed by institutional documents as well. For the analysis of qualitative data, the technique of Content Analysis was adopted. After the analysis, it was found that there is a perception by the protection network members regarding the disrespect to the list of rights of these adolescents. The youth categories of these football clubs permeate the protective legal system, violating their fundamental guarantees constitutionally contemplated (CRFB/1988), as well as its infra-constitutional norms (ECA, Lei Pelé and CLT), even in the administrative norms of sports management entities, such as the CBF and the FPF. However, this veiled and silent structural violence comes to light when complaints of these abuses occur, but above all those with great media repercussion.

Made available in DSpace on 2022-05-01T16:48:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) In tropical no-till systems, applying lime (L) and phosphogypsum (PG) on the soil surface may be a potential strategy for reducing soil acidification and improving soybean root growth, thereby enhancing plant nutrition and physiological responses and, in turn, crop resistance to dry spells. This study evaluated the impact of long-term (17 years) surface soil amendment on soil fertility and soybean root development, nutrition, gas exchange, carbon and antioxidant enzyme activity, and grain yield in a tropical region subject to dry spells. The treatments consisted of the following long-term soil amendments: control (no soil amendment); L alone; PG alone; and L + PG (LPG). Liming, especially when combined with PG, improved soil fertility, as evidenced by increases in pH and P, Ca2+, and Mg2+ levels throughout the soil profile, but reduced Al3+ and micronutrients (Fe, Mn, Cu, and Zn). The improvements in soil fertility were associated with increased root development throughout the profile. Long-term application of LPG reduced the negative impacts of dry spells on pigment concentrations, gas exchange, Rubisco and sucrose synthase activities and antioxidant metabolism, and increased soybean grain yield. Our results reveal that long-term application of LPG is an important approach for increasing the vertical movement of cationic bases and roots in no-till systems to improve soybean nutrition. Long-term amendment with LPG enhanced both carbon and antioxidant metabolism in soybean plants, resulting in higher soybean grain yield, despite the predisposition of this tropical region to dry spells. Department of Crop Science College of Agricultural Sciences São Paulo State University (UNESP), São Paulo Department of Soil Science and Agricultural Engineering State University of Ponta Grossa (UEPG), Paraná Soils Department Center of Rural Sciences (CRS) Federal University of Santa Maria (UFSM), Rio Grande do Sul Department of Biosystems Engineering School of Sciences and Engineering São Paulo State University (UNESP), São Paulo Department of Crop Science College of Agricultural Sciences São Paulo State University (UNESP), São Paulo Department of Biosystems Engineering School of Sciences and Engineering São Paulo State University (UNESP), São Paulo FAPESP: 2018/11063-7 FAPESP: 2019/12764-1 CNPq: 421637/2018-8

This article aims to perform an analysis of the factors that determine the self-perception of oral health of Brazilians, based on a multidimensional methodology basis. This is a cross-sectional study with data from a national survey. A household interview was conducted with a sample of 60,202 adults. Self-perception of oral health was considered the outcome variable and sociodemographic characteristics, self-care and oral health condition, use of dental services, general health and work condition as independent variables. The dimensionality reduction test was used and the variables that showed a relationship were submitted to logistic regression. The negative oral health condition was related to difficulty feeding, negative evaluation of the last dental appointment, negative self-perception of general health condition, not flossing, upper dental loss, and reason for the last dental appointment. The use of a multidimensional methodological basis was able to design explanatory models for the self-perception of oral health of Brazilian adults, and these results should be considered in the implementation, evaluation, and qualification of the oral health network. Resumo O objetivo deste artigo é realizar uma análise dos fatores que determinam a autopercepção de saúde bucal dos brasileiros sob base metodológica multidimensional. Estudo transversal, com dados provenientes de inquérito em âmbito nacional. Foi realizada entrevista domiciliar com uma amostra de 60.202 adultos. Considerou-se a autopercepção de saúde bucal como variável desfecho e características sociodemográficas, de autocuidado e condição de saúde bucal, de utilização de serviços odontológicos, de condição de saúde geral e de trabalho como variáveis independentes. Empregou-se o teste de redução de dimensionalidade e as variáveis que apresentaram relação passaram pelo teste de regressão logística. A autopercepção negativa de saúde bucal apresentou-se relacionada à dificuldade para se alimentar, avaliação negativa do atendimento recebido durante a última consulta odontológica, autopercepção ruim da condição de saúde geral, não utilização de fio dental, perda dental superior e motivo da última consulta com o cirurgião dentista. A utilização de base metodológica multidimensional foi capaz de (re)desenhar modelos explicativos para a condição percebida de saúde bucal de adultos brasileiros, devendo, os seus resultados serem considerados na implementação, avaliação e qualificação da rede de saúde bucal.

The effective utilization of vertical organic transistors in high current density applications demands further reduction of channel length (given by the thickness of the organic semiconducting layer and typically reported in the 100 nm range) along with the optimization of the source electrode structure. Here we present a viable solution by applying rolled-up metallic nanomembranes as the drain-electrode (which enables the incorporation of few nanometer-thick semiconductor layers) and by lithographically patterning the source-electrode. Our vertical organic transistors operate at ultra-low voltages and demonstrate high current densities (~0.5 A cm−2) that are found to depend directly on the number of source edges, provided the source perforation gap is wider than 250 nm. We anticipate that further optimization of device structure can yield higher current densities (~10 A cm−2). The use of rolled-up drain-electrode also enables sensing of humidity and light which highlights the potential of these devices to advance next-generation sensing technologies., For vertical organic field-effect transistors (VOFETs) to reach their potential for display and sensor applications, further improvements to fabrication methods are required. Here, the authors report microfabricated VOFETs featuring rolled-up metallic nanomembrane electrodes and displaying multi-sensing capability.

Made available in DSpace on 2021-06-25T10:21:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Coffee litter and the rain that occurs after the application of pre-emergence herbicides impact on their leaching and weed control effectiveness. The objective of this study was to evaluate the leaching and control effectiveness of indaziflam, applied on coffee litter, and under simulations of different amounts of rainfall. Two experiments in greenhouse were conducted with the application of indaziflam (0.1 kg of active ingredient-a.i.-ha-1), in a com-pletely randomized design. The first involved the leaching of the herbicide using Urochloa plantaginea as a bioindicator, in a 8 x 4 factorial arrangement, with eight depths in the PVC column (0-0.05; 0.05-0.1; 0.1-0.15; 0.15-0.2; 0.2-0.25; 0.25-0.3; 0.3-0.35; 0.35-0.4 m) and four amounts of straw (0; 1; 3 and 5 t ha-1), this factor was isolated for rainfall simulations (10, 20 and 40 mm). The second experiment tested the effectiveness in controlling U. plantaginea, in a 4 x 3 factorial arrangement, with four amounts of litter (0; 1; 3 and 5 t ha-1) and three amounts of rainfall (10, 20 and 40 mm). Both experiments had four repetitions and controls without application of the herbicide. In leaching, a greater effect of indaziflam was found at a depth of 0-0.05 m, and the higher the simulated rainfall, the greater the phytotoxicity observed in U. plantaginea, with 39.75%, 52.50% and 92.06%, for rainfall amounts of 10, 20 and 40 mm, respectively. U. plantaginea showed high susceptibility to control by the herbicide indaziflam, and the lowest control, 82%, was observed when indaziflam was applied on 5 t ha-1 with simulation of rainfall at 10 mm. The increase in the amount of litter on the soil surface, combined with lower amounts of rainfall can reduce the leaching of indaziflam and the control of U. plantaginea. Universidade Federal da Grande Dourados/UFGD Faculdade de Ciências Agrárias Centro Universitário da Fundação de Ensino Octavio Bastos/UNIFEOB Faculdade de Agronomia Universidade Estadual de Londrina/UEL Departamento de Agronomia Universidade Estadual Paulista “Júlio de Mesquita Filho”/UNESP Faculdade de Ciências Agronômicas Universidade Estadual de Ponta Grossa/UEPG Universidade Federal de São Carlos/UFSCar Centro de Ciências Agrárias/CCA Universidade Estadual Paulista “Júlio de Mesquita Filho”/UNESP Faculdade de Ciências Agronômicas

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-05-19T23:01:52Z No. of bitstreams: 1 Joice Rodrigues.pdf: 1921332 bytes, checksum: 7ec09aec76c8646998ee8735e0292f75 (MD5) Made available in DSpace on 2022-05-19T23:01:52Z (GMT). No. of bitstreams: 1 Joice Rodrigues.pdf: 1921332 bytes, checksum: 7ec09aec76c8646998ee8735e0292f75 (MD5) Previous issue date: 2022-03-03 O colostro é o ponto chave para o crescimento do bezerro, fornecendo diferentes nutrientes. Além das imunoglobulinas, responsáveis pela transferência de imunidade passiva, existem os compostos bioativos que promovem diferenciação celular e crescimento intestinal, que auxiliam na ativação de processos metabólicos, promovendo maior absorção de nutrientes e componentes imunológicos. O objetivo do trabalho foi avaliar o efeito das concentrações de insulina, fator de crescimento semelhante á insulina do tipo 1 (IGF-1) e leptina do colostro, com a ocorrência de endoparasitas nos bezerros de corte e a sua relação com as alterações de metabólitos sanguíneos de bezerros. Foram utilizadas 20 fêmeas bovinas, sendo 12 novilhas (Grupo 1) e 8 vacas (Grupo 2) e os animais nascidos dessas fêmeas. Foram colhidas amostras de sangue das mães após o parto (T0U: tempo zero único), nos bezerros ao nascer (T0), 24 h, 48 h e 168 h após a colostragem, para análises bioquímicas dos metabólitos sanguíneos e por teste ELISA a avaliação das concentrações de insulina, IGF-1 e letina. O colostro foi colhido logo após o parto e realizado as análises de seus componentes. As fezes das vacas e novilhas foram colhidas no pré-parto e depois juntamente com a coleta de fezes dos bezerros 30 dias após o nascimento e mensalmente até a desmama, para realização da contagem de ovos por grama de fezes (OPG), coprocultura e identificação das larvas de parasitas gastrointestinais. Para a análise estatística foi realizada análise de variância (ANOVA), para a comparação de duas médias foram feitas análises pelo teste de Tukey, e para a análise das correlações foi realizada a correlação de Pearson. Os BV apresentaram maiores concentrações de colesterol em 24h e 48h após a colostragem (P

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-07-21T16:51:31Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Gustavo Souza Alves.pdf: 2930021 bytes, checksum: daafdb9a5047eca663470055aac86267 (MD5) Made available in DSpace on 2022-07-21T16:51:31Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Gustavo Souza Alves.pdf: 2930021 bytes, checksum: daafdb9a5047eca663470055aac86267 (MD5) Previous issue date: 2022-02-18 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior As células a combustível são definidas como dispositivos com a capacidade de converter energia eletroquímica em energia elétrica e calor, tendo como principal subproduto a água. Diversos tipos e variações de células a combustível são estudadas e já apresentam aplicações práticas, entre estas pode-se encontrar as células a combustível de óxido sólido, caracterizadas pela utilização de material cerâmico como eletrólito. Um dos principais entraves para a utilização é a elevada temperatura de operação, na faixa entre 600°C e 1000°C, reduzindo a vida útil e a gama de aplicações. O avanço dos estudos levou à utilização de óxidos com condução do tipo protônica, abrindo caminho para redução da faixa de temperatura de utilização das células, atingindo temperaturas entre 400°C a 700°C. Estas células passaram a ser conhecidas como células a combustível de óxido sólido com condutividade protônica, e utilizam materiais com estruturas tipo perovskitas. Entre essas, encontram-se compostos como o Ba(Ce, Zr, Y)O3 (BCZY), o qual apresenta características como boa condutividade protônica e boa estabilidade química. Na utilização como ânodos, cermetos baseados em níquel (Ni) são muito estudados, juntando assim este metal com o material utilizado no eletrólito, como o próprio BCZY. Desta forma, esse trabalho estudou a utilização do cermeto Ni-BCZY para a uso em ânodos de células a combustível de óxido sólido com condutividade protônica. O BCZY com composição Ba(Ce0,2Zr0,7Y0,1)O3-δ foi obtido por meio de síntese baseada no método Pechini. A formação do cermeto foi realizada a partir de mistura mecânica por moagem em moinho excêntrico, entre NiO e BCZY, em relações de massa de 60/40, 50/50 e 40/60, respectivamente. As propriedades elétricas foram avaliadas por espectroscopia de impedância, após tratamento térmico para redução do NiO em Ni. Constatou-se a formação do cermeto nas três proporções estudadas, tendo características como a porcentagem de porosidade e condutividade elétrica adequadas para cada proporção. Dentre as composições, as Ni-BCZY 60/40 e Ni-BCZY 50/50 apresentaram valores de condutividade acima de 0,1 S/cm e energias de ativação de 0,02 eV/átomo, demonstrando a viabilidade na utilização como ânodo. Com os resultados apresentados, a composição Ni-BCZY 50/50 foi utilizada para formação de uma célula simétrica, tendo o BCY como eletrólito, verificando assim a formação dessa configuração e o comportamento elétrico desse tipo de célula. The fuel cells are defined as devices with the ability to convert electrochemical energy into electrical energy and heat, having water as the main by-product. Several types and variations of fuel cells are studied and already have practical applications, including solid oxide fuel cells, characterized by the use of ceramic material as electrolyte. One of the main obstacles for its use is the high temperature, remaining between 600°C and 1000°C, reducing its useful life and the range of applications. The progress of the studies led to the use of oxides with proton type conduction, opening the way to reduce the temperature range for cell use, reaching temperatures between 400°C and 700°C. These cells came to be known as protonic ceramic fuel cells, and it uses materials with perovskite-like structures. Therefore, there are compounds such as Ba(Ce, Zr, Y)O3 (BCZY), which has characteristics such as good proton conductivity and good chemical stability. When used as anodes, nickel-based cermets (Ni) are widely studied, thus joining this metal with the material used in the electrolyte, such as BCZY. This work studied the use of Ni-BCZY cermet for use in protonic ceramic fuel cell anodes. The BCZY, with Ba(Ce0,2Zr0,7Y0,1)O3-δ composition, was obtained through synthesis based on the Pechini method. The formation of cermet was performed by a mechanical mixture in eccentric vibrating mill, between NiO and BCZY, in weight ratios of 40/60, 50/50 and 60/40 respectively. The electrical properties were evaluated by impedance spectroscopy, after heat treatment to reduce NiO to Ni. The cermets was observed in the three proportions studied, with characteristics such as the percentage of porosity and electrical conductivity suitable for each proportion. The compositions Ni-BCZY 60/40 and Ni-BCZY 50/50 showed conductivity values above 0.1 S/cm and activation energies of 0.02 eV/atom, demonstrating the feasibility of using as anode. With the results presented, the Ni-BCZY 50/50 composition was used to form a symmetric cell, it having BCY as an electrolyte, thus verifying the formation of this configuration and the electrical behavior of this type of cell.

Made available in DSpace on 2022-04-28T19:51:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aim: This study investigated the effect of denture liners surface modification with Equisetum giganteum (EG) and Punica granatum (PG) on Candida albicans biofilm inhibition supposing its usage as a sustained-release therapeutical delivery system for Candida-associated denture stomatitis. Materials & methods:C. albicans biofilm (SC5314 or ATCC 90028) was formed on soft liners superficially modified by a primer mixed to drugs at minimum inhibitory concentrations (0.100 g for EG and PG or 0.016 g for nystatin per ml of primer). After 24 h, 7 or 14 days, antibiofilm activity was evaluated by colony-forming unit counts. Results: Not all groups were equi-efficient to nystatin after 24 h and 7 days. After 14 days, EG and PG efficacies were not different from nystatin (almost 100% inhibition). Conclusion: The proposed protocol presents a promising option to allopathic drugs for Candida-associated denture stomatitis treatment. Department of Prosthodontics & Periodontics Bauru School of Dentistry University of São Paulo (USP) Department of Surgery Stomatology Pathology & Radiology Bauru School of Dentistry University of São Paulo (USP) Department of Biological Sciences São Paulo State University (UNESP) Department of Dentistry State University of Ponta Grossa (UEPG) PR Department of Biological Sciences São Paulo State University (UNESP) FAPESP: 2016/08702-2, 2017/07314-1

Made available in DSpace on 2022-04-28T19:47:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background and Objective: The indaziflam herbicide has efficiency in the control of monocotyledons weeds and is recommended for some eudicotyledonous species. However, the efficiency of the control can vary to the detriment of the species, dose and soil texture. Therefore, the objective of the present work was to evaluate the effectiveness of the control of indaziflam herbicide for weeds species Mucuna aterrima, Sorghum halepense, Ipomoea purpurea, Rottboellia exaltata, Urochloa decumbens, Merremia aegyptia, Cenchrus echinatus, Digitaria horizontalis, Panicum maximum, Tridax procumbens, Urochloa plantaginea and Eleusine indica, besides elaborating the calculation of DL80, DL90 and DL95 of the product in two types of soil. Material and Methods: Thus, experiments were carried out in a greenhouse, isolated for each weed species, in a completely randomized experimental design, with four replications and a 10×2 factorial scheme, with ten doses of the herbicide indaziflam (0 D, 1/16 D, 1/8 D, 1/4 D, 1/2 D, D, 2 D, 4 D, 8 D, 16 D, being D = 100 g haG1), applied in pre-emergence and two contrasting soil textures (clayey and sandy). At 7, 14, 21, 28, 35, 42, 49 and 56 days after weed emergence (DAE), visual control assessments were performed and in the last evaluation, the dry mass of the aerial part was performed. Results: The weeds of the Poaceae family showed greater susceptibility to the indaziflam herbicide, on the other hand, the Mucuna aterrima, Ipomoea purpurea and Merremia aegyptia weeds required a higher dose of active ingredient to be controlled efficiently. Conclusion: In general, the current study concluded that a lesser amount of active ingredient was needed in sandy soil than in clayey soils to promote adequate weed control. Therefore, the difference in the susceptibility of the studied plants as function of dose indaziflam, weeds species and soil texture. Faculdade de Ciências Agrárias Departamento de Produção Vegetal Escola Superior de Agricultura Luiz de Queiroz/USP Faculdade de Agronomia Universidade do Oeste Paulista Faculdade de Ciências Agronômicas Universidade Estadual Paulista Universidade Estadual de Ponta Grossa/UEPG Departamento de Recursos Naturais e Proteção Ambiental Universidade Federal de São Carlos Faculdade de Ciências Agronômicas Universidade Estadual Paulista

Made available in DSpace on 2022-04-29T08:36:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) B chromosomes are non-essential additional genomic elements present in several animal and plant species. In fishes, species of the genus Psalidodon (Characiformes, Characidae) harbor great karyotype diversity, and multiple populations carry different types of non-essential B chromosomes. This study analyzed how the dispensable supernumerary B chromosome of Psalidodon paranae behaves during meiosis to overcome checkpoints and express its own meiosis-specific genes. We visualized the synaptonemal complexes of P. paranae individuals with zero, one, or two B chromosomes using immunodetection with anti-medaka SYCP3 antibody and fluorescence in situ hybridization with a (CA)15 microsatellite probe. Our results showed that B chromosomes self-pair in cells containing only one B chromosome. In cells with two identical B chromosomes, these elements remain as separate synaptonemal complexes or close self-paired elements in the nucleus territory. Overall, we reveal that B chromosomes can escape meiotic silencing of unsynapsed chromatin through a self-pairing process, allowing expression of their own genes to facilitate regular meiosis resulting in fertile individuals. This behavior, also seen in other congeneric species, might be related to their maintenance throughout the evolutionary history of Psalidodon. Universidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Departamento de Biologia Estrutural e Funcional Universidad de La Serena Instituto de Investigación Multidisciplinar en Ciencia y Tecnología Universidad de La Serena Departamento de Biología Laboratorio de Genética y Citogenética Vegetal Hokkaido University Faculty of Science Department of Biological Sciences Laboratory of Reproductive & Developmental Biology Universidade Estadual Paulista (UNESP) Faculdade de Ciências Departamento de Ciências Biológicas Universidade Federal de São Carlos (UFSCAR) Departamento de Genética e Evolução Universidade Estadual de Ponta Grossa (UEPG) Departamento de Biologia Estrutural Molecular e Genética Universidade Estadual Paulista (UNESP) Instituto de Biociências de Botucatu Departamento de Biologia Estrutural e Funcional Universidade Estadual Paulista (UNESP) Faculdade de Ciências Departamento de Ciências Biológicas FAPESP: 2013/24143-5 FAPESP: 2014/09634-5 FAPESP: 2014/26508-3 FAPESP: 2016/09204-6 FAPESP: 2017/15608-5 FAPESP: 2017/22447-8 FAPESP: 2018/20610-1 CNPq: 306054/2006-0

Made available in DSpace on 2019-10-06T15:49:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-02 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Universidade Estadual Paulista The Fermi-Ulam model consists of a point particle colliding elastically between two hards walls, one is considered fixed and the other one oscillates periodically in time. Most investigations on this model are based on a map approach that, although it is extremely efficient from the computational point of view, ignores processes which are relevant for a thorough dynamical description. The map approach considers the so called static wall approximation. In this paper, by efficiently solving the exact Fermi-Ulam model, we statistically characterize multiple collisions with the moving wall (processes not considered in the static wall approximation) and prove that structures emerging from such collisions have fractal features exhibiting self-similar characteristics. Programa de Pós-Graduação em Ciências/Física Universidade Estadual de Ponta Grossa (UEPG), Câmpus Uvaranas Departamento de Estatística Matemática Aplicada e Computação Universidade Estadual Paulista (UNESP) Departamento de Física Universidade Estadual Paulista (UNESP) Instituto de Física Benemérita Universidad Autónoma de Puebla, Apartado Postal J-48 Departamento de Estatística Matemática Aplicada e Computação Universidade Estadual Paulista (UNESP) Departamento de Física Universidade Estadual Paulista (UNESP) FAPESP: 2013/22764-2 FAPESP: 2017/14414-2 CNPq: 303707/2015-1

Made available in DSpace on 2019-10-04T12:33:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-18 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana) Astyanax is an abundant fish genus in South America. Some species of this group are characterized by the presence of B chromosomes and absence of morphologically differentiated sex chromosomes. In this study, we used quantitative real-time polymerase chain reaction to characterize mRNA expression of dmrt1 in Astyanax scabripinnis gonads. Maturing gonads of males with the B chromosome overexpressed dmrt1. Our findings suggest that B chromosomes may have an adaptive role in A. scabripinnis sex determination and maintenance. Univ Fed Sao Carlos, Dept Genet & Evolucao, Programa Posgrad Biol Evolut & Genet Mol, Sao Carlos, SP, Brazil UPD CJ APTA SAA, Estacao Expt Salmonicultura Campos do Jordao, Sao Paulo, Brazil Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, Sao Paulo, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Programa Posgrad Biol Evolut, Ave Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil CNPq: 407187/2016-2 CAPES: 001 Fundacao Araucaria (Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Parana): 16174

Made available in DSpace on 2022-04-28T19:42:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This research studied the effectiveness of herbicide treatments in weed control and during different periods of emergence, when applied to varying amounts of straw. The experiment was conducted in a greenhouse with pre-emergent herbicides: amicarbazone, metribuzin, indaziflam, isoxaflutole, amicarbazone + indaziflam, metribuzin + indaziflam, and isoxaflutole + indaziflam, against three species (Sorghum halepense, Rottboellia exaltata, and Mucuna aterrima). The experimental design was completely randomized in a 5x4 factorial scheme, with five amounts of straw (0, 1, 2, 3, and 5 t/ha) and four periods of weed emergence (0, 30, 60, and 90 days after treatments). The residual control of indaziflam was influenced by the amount of straw. Metribuzin presented a low residual control, while isoxaflutole was not affected by the amount of straw. Amicarbazone offered residual control for Mucuna aterrima. The association between indaziflam + isoxaflutole displayed a suitable residual control against Sorghum halepense and Rottboellia exaltata. The association of indaziflam + metribuzin adequately controlled Mucuna aterrima and Sorghum halepense. herbicide physical-chemical characteristics can influence their performance, and the association of products may increase residual and weed spectrum control. Univerdade Federal da Grande Dourados UFGD Faculdade de Ciências Agrarias FCA Universidade de São Paulo Escola Superior de Agricultura “Luiz de Queiroz” Universidade Federal de São Carlos/UFSCar Centro de Ciências Agrárias/CCA Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias. Campus Jaboticabal (FCAV/Unesp) Universidade Estadual de Ponta Grossa. UEPG Universidade Estadual Paulista Júlio de Mesquita Filho Campus de Botucatu Faculdade de Ciências Agronômicas Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias. Campus Jaboticabal (FCAV/Unesp) Universidade Estadual Paulista Júlio de Mesquita Filho Campus de Botucatu Faculdade de Ciências Agronômicas FAPESP: 2015/14833-0

Submitted by arlindo kohlrausch (ajfk@uepg.br) on 2022-08-24T22:08:24Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Cristiane Rickli Barbosa.pdf: 4235139 bytes, checksum: 472dec9d4e7be75cfa1bfa6220dd0554 (MD5) Made available in DSpace on 2022-08-24T22:08:24Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Cristiane Rickli Barbosa.pdf: 4235139 bytes, checksum: 472dec9d4e7be75cfa1bfa6220dd0554 (MD5) Previous issue date: 2021-03-15 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior A doença renal crônica (DRC) é uma das principais causas de mortalidade e morbidade, além de um crescente problema de saúde pública. A hemodiálise é o tratamento mais utilizado, contudo, tem sido associada a frequentes intercorrências durante as sessões, onde a forma mais grave é conhecida como síndrome do desequilíbrio da diálise (SDD) que tem como contribuinte o edema cerebral e o aumento da pressão intracraniana (PIC). Estudos anteriores sugerem que a DRC em estágio terminal e a hemodiálise impactem na complacência cerebral, entretanto, a literatura em torno deste assunto é escassa. Neste contexto, este trabalho teve como objetivo realizar um acompanhamento de um grupo de pacientes com DRC (estágio terminal) em hemodiálise através da monitoração da PIC não invasiva antes e ao final das sessões, buscando compreender como este parâmetro se comporta no decorrer do tratamento dialítico, correlacionando os resultados obtidos com o perfil clínico e laboratorial dos pacientes. Tratase de um estudo longitudinal prospectivo, em que foram incluídos 42 pacientes com DRC em estágio terminal em tratamento por hemodiálise três vezes por semana, acompanhados por um período de 6 meses. Também foram incluídos 20 voluntários sem doença renal crônica conhecida, para compor o grupo controle. Foram obtidos dados clínicos e laboratoriais via prontuário médico eletrônico e fichas das sessões de diálise, além de monitorada a PIC de forma não invasiva pelo método Brain4care® antes e ao final das sessões de diálise. Os dados foram tratados através do programa estatístico SPSS 17.0® . Dentro do período de acompanhamento mencionado obteve-se um total de 4.881 monitorações de PIC. Observou-se uma redução dos parâmetros de PIC, time to peak (TTP) e Relação P2/P, pós-diálise, considerando os diferentes momentos (1ª, 2ª e 3ª sessão de diálise) e os diferentes meses de acompanhamento (p0,05, correlação de Person). Houve uma correlação positiva significativa entre os níveis séricos de potássio e os parâmetros TTP e relação P2/P1 (p0.05, Person correlation). There was a significant positive correlation between serum potassium levels and TTP parameters and P2/P1 ratio (p

Made available in DSpace on 2021-06-25T11:14:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Phospholipases-D (PLDs) found in Loxosceles spiders’ venoms are responsible for the der-monecrosis triggered by envenomation. PLDs can also induce other local and systemic effects, such as massive inflammatory response, edema, and hemolysis. Recombinant PLDs reproduce all of the deleterious effects induced by Loxosceles whole venoms. Herein, wild type and mutant PLDs of two species involved in accidents—L. gaucho and L. laeta—were recombinantly expressed and character-ized. The mutations are related to amino acid residues relevant for catalysis (H12-H47), magnesium ion coordination (E32-D34) and binding to phospholipid substrates (Y228 and Y228-Y229-W230). Circular dichroism and structural data demonstrated that the mutant isoforms did not undergo significant structural changes. Immunoassays showed that mutant PLDs exhibit conserved epitopes and kept their antigenic properties despite the mutations. Both in vitro (sphingomyelinase activity and hemolysis) and in vivo (capillary permeability, dermonecrotic activity, and histopathological analysis) assays showed that the PLDs with mutations H12-H47, E32-D34, and Y228-Y229-W230 displayed only residual activities. Results indicate that these mutant toxins are suitable for use as antigens to obtain neutralizing antisera with enhanced properties since they will be based on the most deleterious toxins in the venom and without causing severe harmful effects to the animals in which these sera are produced. Departamento de Biologia Celular Universidade Federal do Paraná Centro de Produção e Pesquisa de Imunobiológicos (CPPI) Departamento de Física Centro Multiusuário de Inovação Biomolecular Universidade Estadual Paulista (UNESP) Departamento de Biologia Estrutural Molecular e Genética Universidade Estadual de Ponta Grossa Departamento de Física Centro Multiusuário de Inovação Biomolecular Universidade Estadual Paulista (UNESP) FAPESP: 2015/13765-0 FAPESP: 2019/10475-2 FAPESP: 2915/18868-2 CNPq: 303868/2016-3 CNPq: 307338/2014-2 CNPq: 309940/2019-2 CNPq: 408633/2018-2

Made available in DSpace on 2021-06-25T12:37:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-11 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) To determine whether the permeation capacity and analgesic efficacy of articaine (ATC) could be increased and cytotoxicity decreased by encapsulation in poly(e-caprolactone) nanocapsules (ATC(nano)), aiming at local or topical anesthesia in dentistry. Cellular viability was evaluated (using the MTT test and fluorescence microscopy) after 1 h and 24 h exposure of HaCaT cells to ATC, ATC(nano), ATC with epinephrine (ATC(epi)), and ATC in nanocapsules with epinephrine (ATC(nanoepi)). The profiles of permeation of 2% ATC and 2% ATC(nano) across swine esophageal epithelium were determined using Franz-type vertical diffusion cells. Analgesic efficacy was evaluated with a von Frey anesthesiometer in a postoperative pain model in rats, comparing the 2% ATC, 2% ATC(nano), 2% ATC(epi), and 2% ATC(nanoepi) formulations to 4% ATC(epi) (a commercially available formulation). We show that use of the nanocapsules decreased the toxicity of articaine (P

Made available in DSpace on 2021-06-25T12:34:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Coordination for the Improvement of Higher Education Personnel Hunting activities are a potential risk factor for human infection with Leptospira spp. and, although wild boar seroprevalence has been studied, there are no concurrent serosurveys of wild boars (Sus scrofa), hunting dogs (Canis lupus familiaris), and hunters. The aim of our study was to assess the seroprevalence of Leptospira spp. antibodies in free-ranging wild boars, hunting dogs, and hunters, and risk factors associated with exposure in southern and central-western Brazil. Leptospira spp. antibodies were serologically detected using the microscopic agglutination test, with a total 30 serovars. Overall, 12.2% (9/74) of wild boars and 10.6% (16/170) of hunting dogs were seropositive for at least one serovar and all hunters 0.0% (0/49) were seronegative for Leptospira spp. Seropositivity was statistically higher in 42.1% (8/19) wild boars from natural areas when compared to 2.4% (1/41) from anthropized areas (P

Made available in DSpace on 2021-06-25T11:55:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Objective: to analyze elderly individuals from different age groups admitted to a teaching hospital, according to sociodemographic characteristics, health and services used. Methods: cross-sectional study, with 158 elderly hospitalized in a university hospital, with dependent variable the age: individuals from 60 to 70 years and more than 70 years; and as an independent variable: sociodemographic, health and service used conditions. Data were analyzed by the Fisher's Exact and chi-square tests. Results: patients older than 70 years had significantly lower level educational, absence of spouse, presence of multimorbities, longer hospitalization, ICU hospitalization and need to help with daily living at home, to than the younger people (p

Made available in DSpace on 2022-05-01T05:29:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 This article aims to identify contributions and limits of classical Greek philosophy to current thoughts on music and musical formation. Through a bibliographic study of works in the field of philosophy, musicology, and education, we analyze the fundamentals on the nature of music and musical formation that appear in Ancient Greek philosophical thought. Next, based mainly on the considerations of György Lukács, we discuss the importance of these conceptions to overcome the subjectivist and formalistic perspectives of music and music education, highlighting the dialectical character of the notion of music as an objective mimesis present in Greek thought. Thus, classical philosophy can help us understand the social meaning of musical practice and its educational processes, showing us ways to reflect on the current condition of music in our society. On the other hand, the idealistic character of these conceptions prevents us from capturing the historical and concrete meaning of this social role, thereby also demonstrating limits to overcome. Universidade Estadual de Ponta Grossa, Ponta Grossa Universidade Estadual Paulista Universidade Estadual Paulista

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:20:53Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:20:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-09-01 The Lagoa Dourada is a circular-shaped pond formed on the Furnas Formation (Devonian of the Paraná Basin), filled by late Pleistocene - Holocene sediments. It lies in the hydrographic basin of the Guabiroba River, a tributary of the Tibagi River situated in the Campos Gerais region of the State of Paraná, southern Brazil. The pond is about 200 m in diameter and the maximum water depth is 5.4 m. Geological, chemical, textural and mineralogical studies on a core sample of the sediments collected from the Lagoa Dourada, 12.2 m thick, was obtained for investigations of paleoenvironmental changes as well as to provide additional data to support previous reconstructions based on palynomorphs and diatoms. Within the period recorded in the core, the pond has been filled by sandy material introduced by springs at the northern edge of the pond as well as by muddy material brought in by floodwaters of the Guabiroba River. Thus, the sandy layers could be interpreted as evidence of drier climates with consequent diminution of fluvial overflow, but with maintenance of the sandy deposits coming from springs. The occurrence of euhedral pyrite in the sediments, locally associated with gypsum, may indicate periods of increase in the organic matter content or an increase in the water salinity, what could be related to greater evaporation under drier paleoclimate regimes. Three cycles defined by an increase in the total carbon content of the sediments of the pond were observed. These cycles seem to correspond to an increase in the isotope ratio 13C/12C (δ13C). Several hypotheses can be suggested to explain the presence of these cycles, including the alternation of wetter or drier climatic phases. The convergence of the sedimentological data obtained during this study with previous microfossil paleoclimatic (pollens and diatoms) indicators and radiocarbon dating suggests that a drier paleoclimatic phase occurred around 8720±150 years B.P. The evidence for a second drier phase in more recent times is less consistent. This subsequent phase could correspond to the drier phase in southern and southeastern Brazil between 5000 and 3000 years B.P. as suggested by other paleoclimatic studies. UEPG-Univ. Estadual de Ponta Grossa, Paraná IGUSP-Inst. de Geociencias du USP, São Paulo CENA-USP-Ctr.Estud.Nucleares Agric., Piracicaba UNESP-Univ. Estadual Paulista, São José do Rio Preto UNESP-Univ. Estadual Paulista, São José do Rio Preto

We are thankful Renato Devidé for his technical assistance and Rafael Henrique Nóbrega and Maeli Dal Pai Silva for the use of their laboratory equipment and Judith Mank for the useful feedback to the manuscript., Background: Eukaryote genomes frequently harbor supernumerary B chromosomes in addition to the “standard” A chromosome set. B chromosomes are thought to arise as byproducts of genome rearrangements and have mostly been considered intraspecific oddities. However, their evolutionary transcendence beyond species level has remained untested. Results: Here we reveal that the large metacentric B chromosomes reported in several fish species of the genus Astyanax arose in a common ancestor at least 4 million years ago. We generated transcriptomes of A. scabripinnis and A. paranae 0B and 1B individuals and used these assemblies as a reference for mapping all gDNA and RNA libraries to quantify coverage differences between B-lacking and B-carrying genomes. We show that the B chromosomes of A. scabripinnis and A. paranae share 19 protein-coding genes, of which 14 and 11 were also present in the B chromosomes of A. bockmanni and A. fasciatus, respectively. Our search for B-specific singlenucleotide polymorphisms (SNPs) identified the presence of B-derived transcripts in B-carrying ovaries, 80% of which belonged to nobox, a gene involved in oogenesis regulation. Importantly, the B chromosome nobox paralog is expressed > 30× more than the A chromosome paralog. This indicates that the normal regulation of this gene is altered in B-carrying females, which could potentially facilitate B inheritance at higher rates than Mendelian law prediction. Conclusions: Taken together, our results demonstrate the long-term survival of B chromosomes despite their lack of regular pairing and segregation during meiosis and that they can endure episodes of population divergence leading to species formation., Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2014/09634-5 2013/24143-5 2017/22447-8 2018/20610-1 2016/09204-6 2014/26508-3, CAPES, National Council for Scientific and Technological Development (CNPq) 306054/2006-0, Spanish Secretaria de Estado de Investigacion, Desarrollo e Innovacion CGL2015-70750-P, Junta de Andalucia, Sven och Lilly Lawskis fond (Sweden), Marie Sklodowska-Curie Individual Fellowship (European Commission) 875732, Uppsala University

Made available in DSpace on 2021-06-25T11:15:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The present study aimed to assess anti-T. gondii antibodies in sheltered and free-roaming cats within a university campus that has an overlapping population of humans and livestock. A total of 51 cats were tested for anti-T. gondii antibodies using the indirect immunofluorescent antibody test. Overall, 8/51 cats (15.7%) were seropositive. Cats were more likely to be seropositive when free-roaming (p= 0.008) and with presence of skin lesions (p= 0.042), and less likely with < 1 year of age (p= 0.021), probably due to higher environmental exposure and infected prey consumption. The presence of seropositive free-roaming cats whose areas overlapped those occupied by humans and livestock may suggest an increased on-campus chance of T. gondii occurrence. Graduate College of Parasitology Federal University of Minas Gerais UFMG Graduate College of Public Heath Federal University of Bahia UFBA Graduate College of Veterinary Medicine São Paulo State University UNESP Graduate College of Molecular Biology Federal University of Paraná UFPR Department of Medicine State University of Ponta Grossa UEPG University of São Paulo USP ESALQ Department of Veterinary Hygiene and Public Health São Paulo State University UNESP College of Veterinary Medicine Purdue University Departament of Veterinary Medicine Federal University of Paraná UFPR Graduate College of Veterinary Medicine São Paulo State University UNESP Department of Veterinary Hygiene and Public Health São Paulo State University UNESP

Made available in DSpace on 2022-04-29T08:41:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-06-05 In association with many proteins, small nuclear RNAs (snRNAs) organize the spliceosomes that play a significant role in processing precursor mRNAs during gene expression. According to snRNAs genic arrangements, two kinds of spliceosomes (major and minor) can be organized into eukaryotic cells. Although in situ localization of U1 and U2 snDNAs have been performed in fish karyotypes, studies with genomic characterization and functionality of U snRNAs integrated into chromosomal changes on Teleostei are still scarce. This study aimed to achieve a genomic characterization of the U snRNAs genes in Apareiodon sp. (2n = 54, ZZ/ZW), apply these data to recognize functional/defective copies, and map chromosomal changes involving snDNAs in Parodontidae species karyotype diversification. Nine snRNA multigene families (U1, U2, U4, U5, U6, U11, U12, U4atac and U6atac) arranged in putatively functional copies in the genome were analyzed. Proximal Sequence Elements (PSE) and TATA-box promoters occurrence, besides an entire transcribed region and conserved secondary structures, qualify them for spliceosome activity. In addition, several defective copies or pseudogenes were identified for the snRNAs that make up the major spliceosome. In situ localization of snDNAs in five species of Parodontidae demonstrated that U1, U2, and U4 snDNAs were involved in chromosomal location changes or units dispersion. The U snRNAs defective/pseudogenes units dispersion could be favored by the probable occurrence of active retrotransposition enzymes in the Apareiodon genome. The U2 and U4 snDNAs sites were involved in independent events in the differentiation of sex chromosomes among Parodontidae lineages. The study characterized U snRNA genes that compose major and minor spliceosomes in the Apareiodon sp. genome and proposes that their defective copies trigger chromosome differentiation and diversification events in Parodontidae. Programa de Pós-Graduação em Genética Universidade Federal do Paraná Centro Politécnico, Avenida Coronel Francisco H. dos Santos, 100, Paraná Departamento de Biologia Estrutural Molecular e Genética Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Paraná Departamento de Genética e Evolução Universidade Federal de São Carlos, Rodovia Washington Luís, Km 235, São Paulo Departamento de Morfologia Instituto de Biociências de Botucatu Universidade Estadual Paulista, Distrito de Rubião Júnior, s/n, São Paulo Departamento de Morfologia Instituto de Biociências de Botucatu Universidade Estadual Paulista, Distrito de Rubião Júnior, s/n, São Paulo

Made available in DSpace on 2021-06-25T10:53:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: Leukotrienes (LTs) participate in the process of tissue damage in periodontal disease by leukocyte chemotaxis and osteoclastic activation. The activation of Cysteinyl-LT receptor is associated with increased expression of proinflammatory molecules and osteoclastogenesis. However, its implications on periodontal disease progression have not been studied. The present study evaluated the effect of the cysteinyl-LT receptor antagonist (montelukast [MT]) on ligature-induced experimental periodontitis (EP) in rats. Methods: Adult male Wistar rats were subjected to bilateral ligature-induced periodontitis and orally treated with MT (at doses of 10 or 30 mg/kg/d, MT10, and MT30, respectively). Sham animals had the ligatures immediately removed and received placebo treatment. Sets of animals were euthanized 7, 14, or 21 days after ligature placement and the mandibles were removed for macroscopic evaluation of alveolar bone loss (ABL). In addition, histological analysis of periodontal tissues, myeloperoxidase (MPO) activity of gingival tissues, and periodontal tissue expression of collagen type I, RUNX2, RANK, RANKL, OPG, BLT1, Cys-LTR1, LTA4H, and LTC4S were also analyzed. Results: MT significantly reduced ABL at 14 (MT10 and MT30) and 21 days (MT10) (P

Made available in DSpace on 2020-12-12T02:45:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 This study aimed to compare the effect of conventional irrigation (CI) and passive ultrasonic irrigation (PUI) with 17% EDTA and QMiX on the maximum depth and percentage of sealer penetration into the dentinal tubules by confocal laser scanning microscopy (CLSM) and to describe the cleaning of root canal walls by scanning electron microscopy (SEM). Eighty single-rooted human mandibular premolars were instrumented and randomly assigned to four groups (n = 20): EDTA + CI, QMiX + CI, EDTA + PUI, and QMiX + PUI. Ten samples from each group were examined by SEM (2,000×) and the remaining 40 roots were filled with a single gutta-percha cone and AH Plus sealer mixed with 0.1% rhodamine B for analysis by CLSM (10×). Images were assessed at distances of 2 mm (apical), 5 mm (middle), and 8 mm (coronal) from the apex with the Leica Application Suite V4.10 software. The EDTA + PUI and QMiX + PUI protocols presented higher rates of debris/smear layer removal in the apical and middle thirds. The PUI was superior to CI in the maximum depth of sealer penetration at the middle third. The QMiX + PUI group had a higher percentage of sealer penetration at the apical third. The PUI and QMiX protocol improved debris/smear layer removal and tubular dentin sealer penetration. Postgraduate Program in Dentistry School of Dentistry Federal University of Uberlândia (UFU) Department of Restorative Dentistry School of Dentistry State University of Ponta Grossa (UEPG) Department of Preventive and Social Dentistry School of Dentistry Federal University of Uberlândia (UFU) Department of Endodontics School of Dentistry Federal University of Uberlândia (UFU) Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp) Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp)

Geographical indications (GI) are part of the existent Intelectual Property instruments. It is a sign used on services or products that have a specific geographical origin and possess qualities or reputation that are due to that origin. It reflects a unique combination of natural resources, cultural assets and history in a given territory. In Brazil, the GI policy is part of the rural development policies. While functioning as product differentiators on the market, GIs also serve to territorial development due to the link with the territory and the collective process needed to its recognition. Thus, this work aimed to analyze witch aspects of development are achieved short-term after the recognition of a geographical indication. The indication of provenance of Serro was chosen due to the period of recognition as well as the importance of the product (artisanal cheese) in Minas Gerais. The exploratory-descriptive research was performed by the case study method with in-depth interviews and documental analysis. In the case studied, the territorial development has not been fully achieved. Impacts related to physical-economic, human and social capital were listed. Nevertheless, the analysis showed that the effectiveness of geographical indications is based on management, commercial, political and social activities. As indicações geográficas (IG) fazem parte dos instrumentos da Propriedade Intelectual. É um signo usado em produtos ou serviços que têm uma origem geográfica especí­fica e possuem qualidades ou reputação que são devidas a essa origem. Reflete uma combinação única de recursos naturais, ativos culturais e história em um determinado território. No Brasil, a polí­tica de IG faz parte das polí­ticas de desenvolvimento rural. Embora funcionem como diferenciadores de produto no mercado, as IGs também servem para o desenvolvimento territorial, devido à ligação com o território e o processo coletivo necessário ao seu reconhecimento. Assim, este trabalho teve como objetivo analisar que aspectos do desenvolvimento são alcançados curto prazo após o reconhecimento de uma indicação geográfica. A indicação da procedência do Serro foi escolhida devido ao perí­odo de reconhecimento e à importância do produto (queijo artesanal) em Minas Gerais. A pesquisa exploratório-descritiva foi realizada pelo método de estudo de caso, com entrevistas em profundidade e análise documental. No caso estudado, o desenvolvimento territorial não foi plenamente alcançado. Os impactos relacionados ao capital fí­sico-econômico, humano e social foram listados. No entanto, a análise mostrou que a eficácia das indicações geográficas é baseada em atividades administrativas, comerciais, polí­ticas e sociais.

A meta-analysis was carried out to evaluate the recommended levels of digestible lysine and its influence on the productive performance of primiparous lactating sows. The database consisted of 26 publications, 138 diets and 3,820 sows was developed from articles on the association between, lysine, crude protein and lactating primiparous sows. The digestible lysine levels of the studies ranged from 0.22 to 1.43% with average values of 0.85% digestible lysine in the diet, with consumption of 41.25 g day-1. The digestible lysine intake of sows was positively correlated (P

Dia 2 de Setembro de 2007, o Jornal da Manhã (JM), de Ponta Grossa, Paraná, apresentava ao leitor uma versão atualizada do projeto gráfico e editorial do periódico mais antigo em circulação contínua na Cidade. Na remodelação, o JM lança um espaço inédito para crítica de mídia na história do jornalismo diário paranaense: a coluna Ombudsman. Era a primeira vez que um diário local/regional criava espaço para análise da produção jornalística no Estado. O presente texto descreve a experiência da criação e manutenção da coluna de ombudsman no JM, até a extinção do espaço de crítica institucional pelo próprio diário. O relato e a descrição do exercício da crítica dialogam com os desafios da implantação da proposta e as dificuldades no fazer cotidiano da análise jornalística. De 02/09/2007 até 30/03/2011, tempo em que a coluna circulou semanalmente no Jornal, na edição de domingo, o espaço contou com a participação de quatro jornalistas que fizeram a crítica diária, em um mandato de 12 meses cada. De forma analítica, o artigo recupera a experiência, no período em que o JM manteve o espaço de crítica (com blog para atualização diária e uma página standard na edição dominical), destacando as características, limites e desafios da análise jornalística, com ênfase no período do primeiro mandato de ombudsman no JM, que compreende os 12 meses iniciais da experiência de crítica (02/09/2007 a 31/08/2008).

Made available in DSpace on 2020-12-10T20:01:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA) Maranhao State Research Foundation (FAPEMA, Brazil) Background Desipramine is a tricyclic antidepressant with immune-modulatory activity, whose effects on ligature-induced periodontitis are yet to be investigated. Hence, its actions on alveolar bone resorption, gingival collagen content and key inflammatory mediators were herewith analyzed. Methods A total of 60 male Wistar rats were randomly assigned into three groups: 1) control: rats without ligature treated with vehicle (saline); 2) ligature: rats with ligature-induced periodontitis treated with vehicle; 3) ligature + desipramine: rats with ligature-induced periodontitis treated with desipramine (20 mg/kg/d in vehicle). Mandibles and gingival tissues were collected 3 or 15 days after ligature insertion (or no ligature insertion for controls) and treatments. Alveolar bone resorption and gingival collagen fibers were histologically analyzed using either HE or picrosirius red staining. Gingival mRNA expressions of interleukin (IL)-1 beta, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinase (TIMP)-1 were obtained through reverse transcription polymerase chain reaction. MMP-9 activity was analyzed by zymography. Results Alveolar bone loss was significantly reduced in the ligature + desipramine group (P < 0.05), whereas gingival collagen degradation was like the ligature group (P > 0.05). Desipramine administration downregulated mRNA expressions of IL-1 beta, iNOS, COX-2, and TIMP-1 when compared to vehicle alone in the ligature group (P < 0.05). MMP-9 expression and MMP-9/TIMP-1 ratio were similar among rats with ligature-induced periodontitis (P > 0.05); however, MMP-9 activity was lower in the group treated with desipramine (P < 0.05). Conclusion Desipramine administration reduced alveolar bone loss as histologically observed, and modulated key bone remodeling and inflammatory mediators in rats with ligature-induced periodontitis. Univ Fed Maranhao, Post Grad Program Dent, Sao Luis, Maranhao, Brazil Univ Estadual Ponta Grossa, Dept Gen Biol, Ponta Grossa, Parana, Brazil Fac Sci Tocantins, Araguaina, Tocantins, Brazil Univ Ceuma, Post Grad Program Dent, Sao Luis, Maranhao, Brazil Univ Fed Maranhao, Sch Med, Sao Luis, Maranhao, Brazil Univ Taubate, Nucleus Periodontal Res, Taubate, SP, Brazil Sao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil Nova Southeastern Univ, Coll Dent Med, Ft Lauderdale, FL 33314 USA Univ Fed Alfenas, Biol Sci Grad Program, Rua Gabriel Monteiroda Silva 700,Predio E Sala, BR-37130001 Alfenas, MG, Brazil Sao Paulo State Univ, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil FAPESP: 2008/00566-6 CAPES: 4073/08-8 National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR, USA): R15 DE027851 Maranhao State Research Foundation (FAPEMA, Brazil): 1818/2012

Made available in DSpace on 2021-06-25T22:04:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-03 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study aimed to evaluate the effect of aqueous extract of Paullinia cupana (AEG) against ketoprofen side effects, through biochemical, hematological, and histological parameters. AEG showed antioxidant activity in the DPPH center dot scavenging (IC50 = 17.00 +/- 1.00 mu g/ml) and HPLC analysis revealed that this extract is constituted by antioxidants (caffeine, catechins, theobromine, and polyphenols). In vivo experiments in female Wistar rats demonstrated that alterations in urea, creatinine, and uric acid levels promoted (p < .05) by ketoprofen were reversed when AEG was co-administered. Ketoprofen significantly decreased the catalase levels of animal tissues (p < .05), which were restored when AEG was co-administered with the mentioned drug. Histological analysis showed that AEG protected tissues from damages caused by ketoprofen. Moreover, AEG reestablished the number of white blood cells, which had decreased when ketoprofen was administered. In conclusion, this study suggested that the association between ketoprofen and AEG may be an alternative to reduce health damages caused by this drug. Practical applications Paullinia cupana, popularly known as guarana, is commonly consumed as a beverage in Brazil and exhibits pharmacological and beneficial effects to humans. Ketoprofen is an efficacious drug employed in the treatment of inflammatory processes. However, this drug can cause several side effects in humans. Thus, the usage of natural products and plant extracts that can reduce such undesirable effects consists in a valuable strategy to be applied in therapeutic interventions. Univ Estadual Ponta Grossa, Dept Anal Clin & Toxicol, Ponta Grossa, Parana, Brazil Univ Estadual Ponta Grossa, Dept Quim, Ponta Grossa, Parana, Brazil Univ Estadual Paulista, Dept Anal Clin, Sao Paulo, Brazil Univ Estadual Paulista, Dept Fisiol & Patol, Sao Paulo, Brazil Univ Fed Santa Catarina, Dept Farmacol, Florianopolis, SC, Brazil Univ Fed Santa Maria, Lab Biogen, Santa Maria, RS, Brazil Univ Fed Sergipe, Dept Farm, Sao Cristovao, Brazil Univ Estadual Paulista, Dept Anal Clin, Sao Paulo, Brazil Univ Estadual Paulista, Dept Fisiol & Patol, Sao Paulo, Brazil

Made available in DSpace on 2020-12-10T19:55:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) National Bank for Economic and Social Development - BNDES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The wide variation found in the size of eukaryotic genomes is largely related to the accumulation of repetitive sequences. Studies show that these sequences can go through an evolutionary process (molecular co-optation) and acquire new genomic functions. Cytogenetic studies reveal a wide karyotypic variation between chelonians (order Testudines) (2n = 26-68), attributed mainly to the number of microchromosomes. The study of repetitive DNAs has the potential to provide data on the dynamics of these sequences, and how they influence the organization of the genome. Here, we reveal the first in situ mapping data of 45S rDNA, histone H3 genes, and telomeric sequences, for a species of the genus Rhinoclemmys, R. punctularia. The karyotype described here for R. punctularia is different from previous reports for the diploid complement of this species, with differences probably attributable to centric fissions and pericentric inversions or centromere repositioning. The 45S rDNA are on a single chromosome pair (like in other turtles), telomeric sequences are in terminal position on all the chromosomes, and histone H3 is dispersed in low copy number, with clusters in pericentromeric regions of three chromosome pairs. We report on the presence of a Gypsy retrotransposon insert located within H3 histone of R. punctularia, and the H3 region sequenced contained the open reading frame of the histone sequence. Comparative modeling revealed a functional pattern for the protein, thus suggesting that the Gypsy element might have been recruited for new functions in the genome of this species. Univ Fed Para, Ctr Estudos Avancados Biodiversidade, Inst Ciencias Biol, Lab Citogenet, Belem, Para, Brazil Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Ponta Grossa, Parana, Brazil Univ Fed Rural Amazonia, Campus Capanema, Capanema, Parana, Brazil Univ Estadual Paulista, Dept Morfol, Botucatu, SP, Brazil Univ Estadual Paulista, Dept Morfol, Botucatu, SP, Brazil CNPq: 475013/2012-3 National Bank for Economic and Social Development - BNDES: 2.318.697.0001 CNPq: 47/2012 CNPq: 305880/2017-9 CNPq: 305876/2017-1

Made available in DSpace on 2020-12-10T20:00:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-15 Despite vulnerability and unsanitary conditions of animal hoarding may predispose environmental contamination and spread of vectors and pathogens, no study to date has focused on their impact on public health and zoonotic diseases. Accordingly, this study aimed to assess the seroprevalence of anti-Toxoplasma gondii antibodies and associated factors in individuals with animal hoarding disorder (AHD) and their dogs in Curitiba, Southern Brazil. Blood samples were obtained from 264 dogs (21 households) and 19 individuals with AHD (11 households). Their blood was tested by indirect fluorescent antibody test (IFAT). Overall, anti-Toxoplasma gondii seropositivity was found in 21/264 dogs (7.95%; 95% CI: 4.69-11.22) with titers ranging from 16 to 4096, and in 7/19 individuals with AHD (36.84%; CI: 15.15-58.53) with titers ranging from 16 to 64. Serological analysis for anti-T. gondii antibodies were considered positive in at least one individual or dog in 9/11 (81.82%; 95% CI: 59.03-100.00) cases that were thoroughly assessed. Surprisingly, the seropositivity of individuals with AHD and their dogs was among the lowest reportedly observed in human and dog populations of Brazil. There was no significant association between positive owners and positive dogs or the presence of cats in the household. Regard epidemiological variables, a significant association was found between dog's seropositivity and the type of dog food. To the authors' knowledge, the present study represents the first investigation of T. gondii seroprevalence in individuals with hoarding disorder and their dogs. In conclusion, despite low sanitary conditions, anti-Toxoplasma gondii antibodies frequency in individuals with AHD and their dogs are lower than the general population likely due to low protozoan load in such isolated households. Univ Fed Parana, Dept Vet Med, Curitiba, Parana, Brazil Univ Fed Bahia, Inst Collect Hlth, Salvador, BA, Brazil Univ Estadual Ponta Grossa, Dept Nursing & Publ Hlth, Ponta Grossa, Parana, Brazil AAC&T Res Consulting, Curitiba, Parana, Brazil Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil Purdue Univ, Coll Vet Med, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA Univ Fed Parana, Dept Collect Hlth, Curitiba, Parana, Brazil Secretary Environm, Curitiba City Hall, Curitiba, Parana, Brazil Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, Botucatu, SP, Brazil

Made available in DSpace on 2020-12-12T01:11:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 In the previous study, the effect of chemical modification with sodium tripolyphosphate on the properties of corn and pinhão starches was evaluated. However, physical modifications also confer specific characteristics on the starch, diversifying its application. Thus, in this study, thermal (thermogravimetry [TG], differential exploratory calorimetry [DSC]), structural (X-ray powder diffractometry [XRD], Fourier-transform infrared [FTIR]) and morphological (field emission gun-scanning electron microscopy) properties of native and phosphated corn and pinhão starches are evaluated before and after ball milling treatment. In addition, wavelength dispersive X-ray spectroscopy is applied as a new technique to measure the level of phosphorus and sodium. The incorporation of phosphate groups in starches is confirmed and phosphorus and sodium are reduced after milling. The doubly modified starches show lower moisture retention capacity and lower thermal stability. All treatments reduce the gelatinization enthalpy mainly for double modified starches. Relative crystallinity is drastically reduced after the double modification. Changes in FTIR results are more pronounced after the physical treatment, corroborating with DSC and XRD results. Morphological changes are observed only in the milled samples. Thus, the double modification confer greater changes compared to the isolated modifications. State University of Ponta Grossa (UEPG), Av. Carlos Cavalcanti, 4748–Uvaranas Federal Institute of Education Science and Technology of Santa Catarina (IFSC), Av. Expedicionários, 2150–Campo da Água Verde Paulista State University–UNESP–Campus Bauru, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01–Vargem Limpa Paulista State University–UNESP–Campus Bauru, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01–Vargem Limpa

Made available in DSpace on 2022-05-01T00:16:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 In this paper, we investigated the importance of the peroxidase in the enzymatic reactions of living organisms, analyzing the dynamic behavior of a mathematical model that describes the dynamics of the kinetic reaction of such an enzyme system. The methodology used for the dynamic analysis of the model was the maximum Lyapunov Exponent and the Bifucation Diagram, which allowed the determination of the chaotic and periodic regions of the system in addition to the analysis of Shannon's entropy in which the parameters that maximize and minimize the information entropy. We obtained as results the regions in which the parameters k1 and k3 are periodic or chaotic, confirming those found by the exponent of Lyapunov and the bifurcation diagram. Considering that the mathematical model has parametric sensitivity for certain values of the parameters of the peroxidase enzyme reaction constants. Universidade Tecnologica Federal do Parana (UTFPR) Universidade Estadual de Ponta Grossa (UEPG) Universidade Estadual Paulista 'Julio de Mesquita Filho' Universidade Estadual Paulista 'Julio de Mesquita Filho'

Full list of authors: Souami, D.; Braga-Ribas, F.; Sicardy, B.; Morgado, B.; Ortiz, J. L.; Desmars, J.; Camargo, J. I. B.; Vachier, F.; Berthier, J.; Carry, B.; Anderson, C. J.; Showers, R.; Thomason, K.; Maley, P. D.; Thomas, W.; Buie, M. W.; Leiva, R.; Keller, J. M.; Vieira-Martins, R.; Assafin, M.; Santos-Sanz, P.; Morales, N.; Duffard, R.; Benedetti-Rossi, G.; Gomes-Júnior, A. R.; Boufleur, R.; Pereira, C. L.; Margoti, G.; Pavlov, H.; George, T.; Oesper, D.; Bardecker, J.; Dunford, R.; Kehrli, M.; Spencer, C.; Cota, J. M.; Garcia, M.; Lara, C.; McCandless, K. A.; Self, E.; Lecacheux, J.; Frappa, E.; Dunham, D.; Emilio, M. .--Open Access article, published by EDP Sciences, under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited., Context. We present results from the first recorded stellar occultation by the large trans-Neptunian object (174567) Varda that was observed on September 10, 2018. Varda belongs to the high-inclination dynamically excited population, and has a satellite, Ilmarë, which is half the size of Varda. Aims. We determine the size and albedo of Varda and constrain its 3D shape and density. Methods. Thirteen different sites in the USA monitored the event, five of which detected an occultation by the main body. A best-fitting ellipse to the occultation chords provides the instantaneous limb of the body, from which the geometric albedo is computed. The size and shape of Varda are evaluated, and its bulk density is constrained using Varda's mass as is known from previous works. Results. The best-fitting elliptical limb has semi-major (equatorial) axis of (383 ± 3) km and an apparent oblateness of 0.066 ± 0.047, corresponding to an apparent area-equivalent radius R′equiv = (370±7) km and geometric albedo pv = 0.099 ± 0.002 assuming a visual absolute magnitude HV = 3.81 ± 0.01. Using three possible rotational periods for the body (4.76, 5.91, and 7.87 h), we derive corresponding MacLaurin solutions. Furthermore, given the low-amplitude (0.06 ± 0.01) mag of the single-peaked rotational light-curve for the aforementioned periods, we consider the double periods. For the 5.91 h period (the most probable) and its double (11.82 h), we find bulk densities and true oblateness of ρ = (1.78 ± 0.06) g cm-3, ϵ = 0.235 ± 0.050, and ρ = (1.23 ± 0.04) g cm-3, ϵ = 0.080 ± 0.049. However, it must be noted that the other solutions cannot be excluded just yet. © D. Souami et al. 2020., This campaign was carried out within the "Lucky Star" umbrella that agglomerates the efforts of the Paris, Granada and Rio teams. It is funded by the European Research Council under the European Community's H2020 (2014-2020/ERC Grant Agreement No. 669416). The following authors acknowledge the respective CNPq grants: F.B.-R. 309578/2017-5; R.V.M. 304544/2017-5, 401903/2016-8; J.I.B.C. 308150/2016-3 and 305917/2019-6; M.A. 427700/2018-3, 310683/2017-3, 473002/2013-2. This study was financed in part by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior -Brasil (CAPES) -Finance Code 001 and the National Institute of Science and Technology of the e-Universe project (INCT do e-Universo, CNPq grant 465376/2014-2). G.B.R. acknowledges CAPES-FAPERJ/PAPDRJ grant E26/203.173/2016, MA FAPERJ grant E-26/111.488/2013 and ARGJr FAPESP grant 2018/11239-8. J.L.O., P.S.-S., N.M., and R.D. acknowledge financial support from the State Agency for Research of the Spanish MCIU through the "Center of Excellence Severo Ochoa" award for the Instituto de Astrofisica de Andalucia (SEV-2017-0709). P.S.-S. acknowledges financial support by the Spanish grant AYA-RTI2018-098657-J-I00 "LEO-SBNAF" (MCIU/AEI/FEDER, UE). Observations from the RECON network were provided by students, teachers, and community members, including Xavier Banaga, Jesus Bustos, Amanda Carrillo, Dorey W. Conway, Kenneth Conway, Danielle D. Laguna, Andrew E. McCandless, Kaitlin McArdle, and Jared T. White, Jr. The observers listed in this paper are but a small fraction of the total RECON network and their dedication to this project is deeply appreciated. Funding for RECON was provided by grants from NSF AST-1413287, AST-1413072, AST-1848621, and AST-1212159. This work has made use of data from the European Space Agency (ESA) mission Gaia (https://www.cosmos.esa.int/gaia), processed by the Gaia Data Processing and Analysis Consortium (DPAC, https://www.cosmos.esa.int/web/gaia/dpac/consortium).Funding for the DPAC has been provided by national institutions, in particular the institutions participating in the Gaia Multilateral Agreement. This research has made use of the VizieR catalogue access tool, CDS, Strasbourg, France (DOI: 10.26093/cds/vizier). The original description of the VizieR service was published in Ochsenbein et al. (2000).