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Background: Upland rice varieties exhibit significant genetic diversity and broad environmental adaptability, making them ideal candidates for identifying consistently expressed stress-responsive genes. F-box proteins typically function as part of the SKP1-CUL1-F-box protein (SCF) ubiquitin ligase complexes to precisely regulate gene expression and protein level, playing essential roles in the modulation of abiotic stress responses. Therefore, utilizing upland rice varieties for screening stress-responsive F-box genes is a highly advantageous approach. Results: Through mRNA-seq analysis in the Brazilian upland rice (cv. IAPAR9), the research identified 29 drought-responsive F-box genes. Gene distribution and duplication analysis revealed these genes are distributed on 11 of the 12 chromosomes and 10 collinear gene pairs were identified on different chromosomes. 13 cis-elements or binding sites were identified in the promoters of the 29 drought-responsive F-box genes by analysis. Protein domain, stability and subcellular localization analysis results suggest that these F-box proteins possess F-box domain and several other domains, and they are mostly unstable proteins with subcellular localization in cytoplasm, nucleus, chloroplasts, mitochondria and endoplasmic reticulum. Most of drought-responsive F-box genes exhibited expression in various tissues such as root, stem, leaf, leaf sheath and panicle except for OsFBO10 and OsFBX283. These genes exhibited various responses to abiotic stresses such as osmotic, cold, heat, and salt stresses, along with ABA treatment. Importantly, a frame-shift mutation in OsFBX148 was created in the ZH11 variety, leading to altered ABA signal transduction and ROS accumulation. The study further elucidated the interaction of OsFBX148 with SKP1 family proteins OSK4/7/17 to form the SCF complex, dependent on the F-box domain. Conclusions: The research identified and analyzed 29 drought-responsive F-box genes in upland rice and provides valuable insights into the role of OsFBX148 in ABA and ROS responses. It establishes a basis for future exploration of F-box genes in improving resistance to abiotic stresses, especially drought. [ABSTRACT FROM AUTHOR]

Rice ranks as the third most important staple cereal food crop in Kenya, following maize and wheat. The demand for rice has been increasing at a faster rate than most cereals, occasionally requiring imports to meet local needs. In response, the Kenyan government has been implementing a programme to increase rice production by expanding irrigated lowland rice schemes from 32 988 to 171 676 ha, while increasing rain-fed upland production areas four-fold to 42 000 ha by 2030. However, with continued rice monocropping in irrigated lowland fields and intensification in rain-fed upland ecosystems, there is a growing concern that plant-parasitic nematodes may emerge as a significant problem across rice ecosystems. This study characterised the nematode genera associated with rice in Kenya and assessed their diversity and distribution across rice ecosystems, with a focus on Hirschmanniella species. Eleven nematode genera were identified in both ecosystems, indicating a relatively high diversity. These genera, ranked by prominence, included Hirschmanniella, Hoplolaimus, Pratylenchus, Helicotylenchus, Meloidogyne, Ditylenchus, Trichodorus, Xiphinema, Rotylenchus, Longidorus and Paratylenchus. The family Tylenchidae was also found to be an important component of the nematofauna. Both morphological and molecular analyses confirmed the presence of Hirschmanniella oryzae in irrigated lowland and rain-fed upland rice, which represents the first documented report for Kenya. A notably large molecular variation was also observed for these Kenyan populations on the 18S region, with intraspecific variation up to 10 bp difference. Understanding the diversity, distribution, and species composition of nematodes in rice ecosystems is important for raising awareness among plant protection services, farmers, cooperatives and extension agents about plant-parasitic nematodes and their challenges in rice production. Future research should build on this knowledge to support extensive studies on population dynamics and damage threshold, focusing on local rice cultivars to develop effective management strategies, including breeding programmes and other cultural practices. [ABSTRACT FROM AUTHOR]

Rotational shifting cultivation (RSC) is commonly practiced in northern Thailand for upland rice cultivation, primarily for household consumption. However, the potential health risks from heavy metal contamination in these soils have not been thoroughly explored. This study aimed to evaluate the contamination of six heavy metals (Arsenic (As), Mercury (Hg), Cadmium (Cd), Lead (Pb), Copper (Cu), and Chromium (Cr)) in upland rice across RSC fields with varying fallow periods and assess the associated health risks from rice consumption. Four RSC fields with 5, 6, 10, and 12-year fallow periods were examined. The RSC-6Y and RSC-12Y fields were used for upland rice cultivation in 2022, while the RSC-5Y and RSC-10Y fields were cultivated in 2023. The geo-accumulation index (Igeo) was calculated, and translocation factors (TF) were assessed for the transfer of heavy metals from soil to straw (TFStraw/Soil), straw to grain (TFGrain/Straw), and soil to grain (TFGrain/Soil). The results indicated that after burning vegetation in the RSC fields, the highest concentrations of Pb, Cr, and Hg were found in the ash. In RSC soils, Cu, Cr, As, Pb, and Hg levels were below reference standards, with Cd undetected. In rice grains, the order of concentration was Pb > Cu > Cr > As, with Hg and Cd undetected. Pb levels in rice grains exceeded the safety threshold. Igeo values indicated no contamination to moderate contamination across sites, with negative Igeo values for Cr and Cu, and zero values for Cd. The TF results showed limited transfer of As, Hg, and Cd from soil to rice plants (TFStraw/Soil < 0.1), but notable transfer for Pb, Cr, and Cu. Pb was readily transferred from soil to grain (TFGrain/Soil), posing a potential health risk. The study highlights potential non-carcinogenic and carcinogenic risks from heavy metal exposure, particularly Pb, and underscores the need for further research to determine contamination sources and mitigation strategies. [ABSTRACT FROM AUTHOR]

Under the one-season-a-year cropping pattern in Northeast China, continuous cropping is one of the main factors contributing to the degradation of black soil. Previous studies (on maize–soybean, maize–peanut, and maize–wheat intercropping) have shown that intercropping can alleviate this problem. However, it is not known whether intercropping is feasible for maize and rice under dry cultivation, and its effects on yield and soil fertility are unknown. A three-year field-orientation experiment was conducted at Jilin Agricultural University in Changchun city, Jilin Province, China, consisting of three cropping regimes, namely rice under dry cultivation–maize intercropping (IRM), sole rice under dry cultivation (SR), and sole maize (SM). All straw was fully returned to the field after mechanical harvesting. Rice under dry cultivation–maize intercropping with a land-equivalent ratio of 1.05 (the average of three years values) increased the total yield by 8.63% compared to the monoculture system. The aggressivity (A), relative crowding coefficient (K), time–area-equivalent ratio (ATER), and competition ratio (CR) value were positive or ≥1, also indicating that the rice under dry cultivation–maize intercropping had a yield advantage of the overall intercropping system. This is because the intercropped maize root length density (RLD) increased by 33.94–102.84% in the 0–40 cm soil layer, which contributed to an increase in the soil porosity (SP) of 5.58–10.10% in the 0–30 cm soil layer, an increase in the mean weight diameter of soil aggregates (MWD) of 3.00–15.69%, an increase in the geometric mean diameter of soil aggregates (GMD) of 8.16–26.42%, a decrease in the soil bulk density (SBD) of 4.02–7.35%, and an increase in the soil organic matter content (SOM) of 0.60–4.35%. This increased the water permeability and aeration of the soil and facilitated the absorption of nutrients and water by the root system and their transportation above ground, and the plant nitrogen, phosphorus, and potassium accumulation in the intercropping system were significantly higher than that in monoculture treatment, further promoting the total yield of intercropping. This suggests that rice under a dry cultivation–maize intercropping system is feasible in Northeast China, mainly because it promotes belowground root growth, improves the soil environment, and increases the total yield of intercropping. [ABSTRACT FROM AUTHOR]

In the field of agriculture, the post-harvest stage often presents a multitude of obstacles that have the potential to undermine the quality and longevity of the crop. One of the primary obstacles in rice production is the effective drying of rice grains, which is crucial for preventing problems such as mold growth, discoloration, and unintentional germination. To tackle this issue, we propose an innovative solution: a rice grain drying system driven by an Arduino microcontroller. This device effectively combines technology accuracy with agricultural needs. The system is equipped with a very precise temperature sensor to maintain a uniform drying environment, fluctuating within the range of 50–60 °C. The use of a nichrome heater, selected for its consistent and dependable heat production, is complemented by an air blower to ensure the even distribution of heat throughout the drying chamber. One notable characteristic of this innovation is its significant drying capacity of 50 kg in each operation. The effectiveness of the product was verified by a rigorous testing process. A batch of glutinous rice weighing 25 kg, initially containing 25.5% moisture, was successfully reduced to 13.5% moisture content within a time frame of 125 min. In a similar vein, a batch of grains weighing 40 kg, which had just undergone threshing, had a moisture content of 22% initially. Remarkably, this batch achieved the desirable moisture level of 14% within a mere two-hour timeframe. This innovative technology not only provides a resolution to the difficulties faced in post-harvest processes but also signifies a fundamental change in thinking, introducing a fresh era where intelligent technology seamlessly integrates with traditional agricultural methods. [ABSTRACT FROM AUTHOR]

ABSTRACT The recent invasion of the fall armyworm (FAW) into Asia not only has had a major impact on maize yield but is feared to also pose a risk to rice production. We hypothesized that the brown planthopper (BPH) may aggravate this risk based on a recently discovered mutualism between the planthopper and the rice striped stem borer. Here we show that BPH may indeed facilitate a shift of FAW to rice. FAW females were found to strongly prefer to oviposit on BPH‐infested rice plants, which emitted significantly elevated levels of five volatile compounds. A synthetic mixture of these compounds had a potent stimulatory effect on ovipositing females. Although FAW caterpillars exhibited relatively poor growth on both uninfested and BPH‐infested rice, a considerable portion completed their development on young plants. Moreover, FAW were found to readily pupate and survive in exceedingly moist soils typical for rice cultivation, further highlighting FAW's potential to switch to rice. We conclude that BPH, by changing the bouquet of volatiles emitted by rice plants, may greatly facilitate this switch. These findings, together with a current increase of nonflooded upland rice in Asia, warrant careful monitoring and specific control measures against FAW to safeguard Asian rice production. [ABSTRACT FROM AUTHOR]

Drought is a major environmental stress limiting crop yields worldwide. Upland rice (Oryza sativa) has evolved complex genetic mechanisms for adaptative growth under drought stress. However, few genetic variants that mediate drought resistance in upland rice have been identified, and little is known about the evolution of this trait during rice domestication. In this study, using a genome-wide association study we identified ROOT LENGTH 1 (RoLe1) that controls rice root length and drought resistance. We found that a G-to-T polymorphism in the RoLe1 promoter causes increased binding of the transcription factor OsNAC41 and thereby enhanced expression of RoLe1. We further showed that RoLe1 interacts with OsAGAP, an ARF-GTPase activating protein involved in auxin-dependent root development, and interferes with its function to modulate root development. Interestingly, RoLe1 could enhance crop yield by increasing the seed-setting rate under moderate drought conditions. Genomic evolutionary analysis revealed that a newly arisen favorable allelic variant, proRoLe1 −526T, originated from the midwest Asia and was retained in upland rice during domestication. Collectively, our study identifies an OsNAC41-RoLe1-OsAGAP module that promotes upland rice root development and drought resistance, providing promising genetic targets for molecular breeding of drought-resistant rice varieties. A genome-wide association study identified a root length and drought resistance-related gene RoLe1 , which is directly activated by OsNAC41. RoLe1 interacts with OsAGAP and decreases its abundance, leading to a reduced influx rate of IAA in rice roots. Introducing a favorable RoLe1 haplotype into drought-sensitive rice varieties significantly enhances drought resistance. [ABSTRACT FROM AUTHOR]

Background and Aims Root system architecture (RSA) plays a key role in plant adaptation to drought, because deep rooting enables better water uptake than shallow rooting under terminal drought. Understanding RSA during early plant development is essential for improving crop yields, because early drought can affect subsequent shoot growth. Herein, we demonstrate that root distribution in the topsoil significantly impacts shoot growth during the early stages of rice (Oryza sativa) development under drought, as assessed through three-dimensional image analysis. Methods We used 109 F12 recombinant inbred lines obtained from a cross between shallow-rooting lowland rice and deep-rooting upland rice, representing a population with diverse RSA. We applied a moderate drought during the early development of rice grown in a plant pot (25 cm in height) by stopping irrigation 14 days after sowing. Time-series RSA at 14, 21 and 28 days after sowing was visualized by X-ray computed tomography and, subsequently, compared between drought and well-watered conditions. After this analysis, we investigated drought-avoidant RSA further by testing 20 randomly selected recombinant inbred lines in drought conditions. Key Results We inferred the root location that most influences shoot growth using a hierarchical Bayes approach: the root segment depth that impacted shoot growth positively ranged between 1.7 and 3.4 cm in drought conditions and between 0.0 and 1.7 cm in well-watered conditions. Drought-avoidant recombinant inbred lines had a higher root density in the lower layers of the topsoil compared with the others. Conclusions Fine classification of soil layers using three-dimensional image analysis revealed that increasing root density in the lower layers of the topsoil, rather than in the subsoil, is advantageous for drought avoidance during the early growth stage of rice. [ABSTRACT FROM AUTHOR]

The article "Who (Will) Eat Rice in the United States?" published in Choices Magazine discusses the importance of rice in global food security and its significance in the United States. It highlights the demographic drivers of rice demand, emphasizing the diversity of the U.S. population and its impact on rice consumption. The study reveals that Asian and Hispanic individuals are the primary rice consumers in the U.S., with consumption patterns varying by ethnicity and location. The article suggests that policymakers and rice producers should consider the changing population structure when addressing future rice demand. [Extracted from the article]

This study aimed to determine the effect of Mimosa invisa L. weed extract application and a low dose of NPK fertilizer on the intensity of pest attack and pathogen infection on upland rice. This research has been done using Split Plot Design with 3 replications. The main plot was the dose of NPK fertilizer (50% and 100% NPK). The subplot was the concentration of weed extract (0, 1.25, 1.67, 2.5, and 5% concentrations). The variables observed include the intensity of grasshopper and case worm attack, Cercospora sp. that causes brown spots, and Xanthomonas oryzae pv. oryzae that causes bacterial leaf blight. The results showed that the application of weed extract under a low dose (50% NPK) has not been able to suppress the intensity of grasshopper and case worm attacks, and the intensity of the pathogen causes brown spots and bacterial leaf blight. However, 2,5% and 5% concentrations of weed extract can potentially suppress pathogen infection that causes brown spots on upland rice. [ABSTRACT FROM AUTHOR]

The rice dry direct-seeding planting mode is a typical shallow sowing operation, and the traditional compacting mechanism with only longitudinal profiling ability is difficult to ensure the seedbed uniformity, resulting in the seedling emergence rate always lower than 80%. This study innovatively proposed a novel bidirectional-micro-profiling compacting device (BMPCD). In this study, the coupled DEM-MFBD simulation technique was utilised to find that the core design parameters k (elasticity coefficient of the reset spring) and t (thickness of the elastic sheet) of the BMPCD would significantly affect the seedbed uniformity by changing the resistance value F r during the profiling process (P ≤ 0.01). The simulation results showed that when k was taken as 7.8 N mm−1 and t was taken as 1.6 mm, the seedbed uniformity could be most greatly improved. The field experiments showed that compared with the bidirectional profiling compacting device (BPCD) and longitudinal profiling compacting device (LPCD), BMPCD could reduce the coefficient of variation of soil firmness (CVSF) by 33.1% and 40.1%, and the coefficient of variation of sowing depth (CVSD) by 37.1% and 51.8%, respectively, and then improve the seedling emergence rate of dry direct-seeded rice by 5.8% and 12.2%. This indicated that bidirectional and micro-profiling compaction technology could tackle the problem of low seedling emergence rate in rice dry direct-seeding. Meanwhile, the results of the DEM-MFBD coupling simulation were not significantly different from the test results of the field experiments (P > 0.05), indicating that it could be used as an efficient and accurate new method to study the dynamic characteristics between the soil and machinery. • The BMPCD can improve the compacting stability of rice dry direct-seeding. • The DEM-MFBD coupling simulation can realise high precision optimisation of BMPCD. • The BMPCD can increase the seedling emergence rate of dry direct-seeded rice by 12.2%. [ABSTRACT FROM AUTHOR]

Ensuring accurate classification of rice as either cooked or dry is vital for food safety, as improperly stored or cooked rice contain harmful bacteria, emphasizing the importance of maintaining and monitoring food safety standards. In the field of image analysis and food classification, classifying dry and cooked rice samples using photographs is an interesting but difficult task. The main challenge stems from the minute visual variations between cooked and dry rice, which has not always displayed distinct traits that are easily observable by machines. Hence, various machine learning algorithms were implemented to effectively mitigate this issue. However, the existing works have not analysed the physicochemical characteristics due to non-destructive type of experimentation method with image processing. To overcome this issue, this work develops the Classification and Regression Tree (CART) of Decision Tree Learning method for classifying the rice grain samples as dry or cooked based on the physicochemical characteristics such as morphological, texture and color features, which in turn gain an exhaustive facts of rice quality in diverse state. Initially, the images are captured, pre-processed and the features are extracted. From the extracted features, the rice samples are classified as dry and cooked using DT and the results are compared with the existing algorithms like K-Nearest Neighbour (KNN) and Support Vector Machine (SVM). The comparative analysis of these classification algorithms infers the outperformance of the DT learning model under morphological, texture and color features in terms of accuracy, error, precision, recall and F-score. [ABSTRACT FROM AUTHOR]

Purpose: Incorporating rice straw into upland has been adopted as a strategy to increase crop productivity and decrease poisoning to rice from reducing substances accumulation as well as mitigate methane emission from paddy fields in South China. However, the mechanism underlying long-term ex situ incorporation of rice straw in upland on soil organic carbon (SOC) and crop yields through microbial metabolism remains unclear. Materials and methods: Hence, a field experiment was established to investigate the response mechanism of SOC and crop yields underlying microbe-mediated carbon dynamics with nutrient stoichiometry by ex situ incorporation of rice straw in upland. Results and discussion: The results showed that the treatment of cropping with chemical fertilizers plus rice straw (SCF) had the highest SOC accumulation rate (0.14 ± 0.03 g C kg−1 yr−1) during the 22-year experimental period. The mean yields of rapeseed and sweet potato were increased by 5.0% and 4.7% in the SCF treatment, correspondingly, compared with the treatment of cropping with chemical fertilizers (CF). Additionally, based on ecoenzymatic stoichiometry, soil microorganisms were found to be co-limited by carbon and phosphorus, which was aggravated by the decrease of soil available nutrients. The SCF treatment exhibited a higher soil microbiomass, resulting in an increased secretion of ecoenzymatic activities to mediate stoichiometric imbalance and mitigate nutrient competition between plant and microbial nutrient limitations. The findings revealed a significant association between stoichiometric imbalance and both SOC and crop yields, respectively. Conclusions: Therefore, our study indicated that long-term ex situ incorporation of rice straw in upland could be adopted as an effective agricultural management strategy to improve SOC and crop yields. [ABSTRACT FROM AUTHOR]

Fucus spp. seaweeds thrive in the cold temperate waters of the northern hemisphere, specifically in the littoral and sublittoral regions along rocky shorelines. Moreover, they are known to be a rich source of bioactive compounds. This study explored the valorization of Fucus spiralis through the extraction of bioactives and polysaccharides (PSs) for food applications and biostimulant use. The bioactives were extracted using microwave hydrodiffusion and gravity (MHG), where the condition of 300 W for 20 min resulted in the highest total phenolic content and antioxidant activity of the extract. Cellular assays confirmed that the extract, at 0.5 mg/mL, was non-cytotoxic to HaCat cells. Polysaccharides (PSs) were extracted from the remaining biomass. The residue from this second extraction contained 1.5% protein and 13.35% carbohydrates. Additionally, the free amino acids and minerals profiles of both solid residues were determined. An edible film was formulated using alginate (2%), PS-rich Fucus spiralis extract (0.5%), and F. spiralis bioactive-rich extract (0.25%). The film demonstrated significant antioxidant properties, with ABTS and DPPH values of 221.460 ± 10.389 and 186.889 ± 36.062 µM TE/mg film, respectively. It also exhibited notable physical characteristics, including high water vapor permeability (11.15 ± 1.55 g.mm.m−2.day−1.kPa−1) and 100% water solubility. The residues from both extractions of Fucus spiralis exhibited biostimulant (BS) effects on seed germination and seedling growth. BSs with PSs enhanced pea germination by 48%, while BSs without PSs increased the root dry weight of rice and tomato by 53% and up to 176%, respectively, as well as the shoot dry weight by up to 38% and up to 74%, respectively. These findings underscore the potential of Fucus spiralis within the framework of a circular economy, wherein both extracted bioactives and post-extraction by-products can be used for sustainable agriculture and food applications. [ABSTRACT FROM AUTHOR]

The soil‐nitrogen condition, which differs greatly between paddy fields (mainly in the form of ammonium, NH4+) and dry fields (mainly in the form of nitrate, NO3−), is a main environmental factor that drives the adaptive differentiation between upland and lowland rice ecotypes. However, the adaptive differentiation in terms of the nitrogen use efficiency (NUE) between upland and lowland rice has not been well addressed. In this study, we evaluated NUE‐related traits among rice landraces as well as the genetic differentiation between NUE‐associated genes and quantitative trait loci (QTLs). The japonica upland and lowland rice ecotypes showed large differences in their NUE‐related traits such as the absorption ability for NH4+ and NO3−. The indica upland and lowland rice exhibited similar performances when cultivated in solutions containing NH4+ or NO3− or when planted in paddy or dry fields. However, the indica upland rice possessed a greater ability to absorb NO3−. We identified 76 QTLs for 25 measured traits using genome‐wide association analysis. The highly differentiated NUE‐associated genes or QTLs between ecotypes were rarely shared by japonica and indica subspecies, indicating an independent genetic basis for their soil‐nitrogen adaptations. We suggested four genes in three QTLs as the candidates contributing to rice NUE during the ecotypic differentiation. In summary, the soil‐nitrogen condition drives the adaptive differentiation of NUE between upland and lowland rice independently within the japonica and indica subspecies. These findings can strengthen our understanding of rice adaptation to divergent soil‐nitrogen conditions and have implications for the improvement of NUE. [ABSTRACT FROM AUTHOR]

Frequent encounters with the greater bandicoot rats (Bandicota indica) following high rodent damage towards rice crops and lack of information on the species had encouraged this study to be conducted to test the relevance of using first- and second-generation rodenticide in a field efficacy test. This study also attempts to detect any sign of resistance of current rodent pest populations towards chlorophacinone (0.005%) and flucoumafen (0.05%) for the control of field rats predominant rice field agrosystem of the Kedah in northern peninsular Malaysia. Six different treatments over dry and wet rice planting season together with trapping exercise. The observation was evaluated based on the number of active burrows, counting tiller damage due to rodent attack and trapping index. The results indicated that flucoumafen gives better rodent control and has a better impact (p < 0.05) although chlorophacinone is still relevant to be applied (p < 0.05). Treatments during the off-planting season (September-February) are more effective compared to the main planting season (March-August). Rodent control during the early off-planting season is encouraged for better rodent management in the rice field and the use of bait stations to increase the weatherability of the baits. [ABSTRACT FROM AUTHOR]

Background: Rice (Oryza sativa L.) is the major cereal crop grown in India. Rice plays a pivotal role in the socio-cultural life of the people of Assam. The main objective of this study to investigate the influence micro climatic indices in relation to crop growth and yield of upland rice varieties with different dates of sowing in Assam. Methods: A field experiment was carried out at Instructional cum Research Farm, Assam Agricultural University, Jorhat during autumn season of 2017. The experiment consisted of four micro-climatic regimes (M) along with four different rice varieties (V). Result: The results of the experiment revealed that micro climatic indices viz., canopy temperature, growing degree days, soil moisture content, soil temperature, light intensity, depth of ground water table associated with 1st April sown crop recorded positive effect on growth and yield parameters in terms of plant height, leaf area index, number of filled grains per panicle, test weight, harvest index and grain yield (3004 kg/ha), followed by the micro-climate associated with 16th March sown crop. Among the varieties evaluated, CR-Dhan 203 recorded the highest grain yield of 2860 kg/ha and was significantly superior to that of other varieties except Inglongkiri. [ABSTRACT FROM AUTHOR]

Trichoderma fungus isolates have begun to be recognized as biofertilizers, which are expected to not only play a role in breaking down organic material that produces nutrients for plants, but also help plants to live normally in acidic soil conditions and dry land with low light intensity. The initial growth period is a critical phase for upland rice in facing environmental stress including low light intensity and soil acidity. The aim of this research was to determine the effects of providing 60% paranet shading and Trichoderma biological fertilizer on the vegetative growth response of upland rice leaves on acid soil. The experimental treatments were arranged in a split plot design. The primary plot is comprised of two categories: "without shade" and "shade of 60%". The subplots are Trichoderma biological pupils consisting of: without Trichoderma or control, Trichoderma isolate Tc-Jjr-02 and Trichoderma isolate Tc-Jro-02. The treatment was repeated 4 times to obtain a total of 24 experimental units. Weekly observations were made of plant height growth and number of tillers, as well as, the total amount of chlorophyll and the total amount of carotenoids at the end of the vegetative phase. Observational data were analysed using analysis of variance at the 5% level to determine the effect of treatment on the growth response of upland rice. To determine the differences between treatments, an Honest Significant Difference (HSD) test was performed at the 5% level. The results showed that shade, biofertilizer and interaction had no significant effect on plant height, but a very significant effect on number of tillers. Application of Trichoderma biofertilizer without shade showed most tillers and was able to help upland rice plants overcome acid soil stress in the vegetative phase. The shade of 60% can increase 29.8% total chlorophyll and 40% total carotenoids of upland rice leaves. The interaction between shade and Trichoderma biological fertilizer has made a significant impact on the number of tillers in treatments 21 and 56 days after planting. [ABSTRACT FROM AUTHOR]

COVID-19 has caused a deep economic impact on the lives of small and marginal farmers due to travel restrictions, market closures, and social distancing requirements. Due to COVID-induced labor scarcity and water shortage in India, direct-seeded rice (DSR) has emerged as a viable alternative to puddled transplanted rice (PTR). However, there was plenty of labor available in Pakistan and Bangladesh for rice cultivation during COVID-19 times. Therefore, both countries did not observe the shift from PTR to DSR. The cost of inputs, such as seed, fertilizer, pesticide, and fuel, was high due to a supply–demand conflict during the COVID-19 pandemic in three countries. Farmers faced weed problems and physical and/or economical non-availability of suitable machinery for DSR cultivation during the COVID-19 pandemic. In the later years of 2022 and 2023 (post-COVID), the area under DSR decreased by 88% in India, while it remained stagnant in Pakistan and Bangladesh. [ABSTRACT FROM AUTHOR]

Rice (Oryza sativa L.) stands as one of the most critical staple crops globally, with its yield and water use efficiency (WUE) being pivotal for food security. This study aimed to evaluate the agronomic and physiological traits and WUE of six rice varieties under two irrigation regimes: alternate wetting and drying (AWD) and conventional irrigation (CI). The results showed the significant improvements in grain yield and WUE with variety improvement under both irrigation treatments. Under AWD, high water use efficiency varieties (HWVs) demonstrated pronounced enhancements, including tillers and spikelet production, filled grain rate, 1000-grain weight, harvest index, leaf area index, non-structural carbohydrate remobilization, photosynthesis and catalase and peroxidase activities of leaf, root and shoot biomass, and root activity. AWD was observed to synchronize and amplify grain yield (2–14%) and WUE, including both leaf-level (13.94–20.72%) and yield-level (23.20–30.87%) water use efficiencies (WUEL and WUEY). The water use potential for HWVs was substantially enhanced under AWD. The integration of variety improvement with AWD irrigation strategies effectively achieves the dual objectives of high yield and WUE, offering a promising approach for sustainable rice production. [ABSTRACT FROM AUTHOR]

Variations in the cadmium (Cd) accumulation and root characteristics of different genotypes of rice during three developmental periods of dry cultivation were investigated in pot experiments in which two levels of Cd were added to the soil (0 and 10 mg kg−1). The results show that the Cd concentration in each organ of the different rice genotypes decreased in both the order of roots > shoots > grains and during the three developmental periods in the order of the maturity stage > booting stage > tillering stage. The lowest bioaccumulation factor (BCF) and translocation factor (TF) were found in Yunjing37 (YJ37) under Cd stress. At maturity, Cd stress inhibited the root length of Dianheyou34 (DHY34) the most and that of Dianheyou 918 (DHY918) the least, also affecting the root volume of DHY34 and Dianheyou615 (DHY615) the most and that of YJ37 and Yiyou 673 (YY673) the least; the inhibition rates were 41.80, 5.09, 40.95, and 10.51%, respectively. The exodermis showed the greatest thickening in YY673 and the lowest thickening in DHY615, while the endodermis showed the opposite result. The rates of change were 16.48, 2.45, 5.10, and 8.49%, respectively. The stele diameter of DHY615 decreased the most, and that of YY673 decreased the least, while the secondary xylem area showed the opposite result; the rates of change were −21.50, −14.29, −5.86, and −26.35%, respectively. Under Cd stress treatment at maturity, iron plaque was extracted using the dithionite–citrate–bicarbonate (DCB) method. The concentration of iron (DCB-Fe) was highest in YJ37, and the concentration of cadmium (DCB-Cd) was lowest in DHY34. YJ37 was screened as a low Cd-accumulating variety. The concentration of available Cd in the rhizosphere soil, iron plaque, root morphology, and anatomy affect Cd accumulation in rice with genotypic differences. Our screening of Cd-accumulating rice varieties provides a basis for the dry cultivation of rice in areas with high background values of Cd in order to avoid the health risks of Cd intake. [ABSTRACT FROM AUTHOR]

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Glyphosate IPA 240 g/L herbicide is known as one of the effective chemical solutions in weed control, especially in no-tillage systems. No-tillage systems offer advantages in upland rice cultivation by reducing soil damage and production costs. However, the main challenge in the application of no-tillage systems is the presence of pre-planting weeds that can compete with plants in the absorption of growth factors such as nutrients, light, and water. This experiment aims to test herbicide doses that effectively suppress weed growth and optimize the growth and yield of upland rice with the no-tillage system. The experiment was conducted from December 2023 to April 2024 at Ciparanje Farm, Faculty of Agriculture, Padjadjaran University, Jatinangor District, Sumedang Regency, West Java Province, Indonesia. The experiment used Randomized Block Design (RBD), consisting of 7 treatments, each repeated four times. The treatments consisted of 5 doses of glyphosate IPA 240 g/L herbicide 2.00, 3.00; 4.00, 5.00; 6.00 L/ha with no-tillage system; manual weeding; and control with perfect tillage system. Experimental results showed that glyphosate IPA 240 g/L herbicide at a dose of 3.00 - 6.00 L/ha with the no-tillage system can be used to replace the perfect tillage system because it can suppress the growth of broadleaf weeds, namely Ageratum conyzoides, Cleome rutidosperma, Mollugo pentaphylla and Synedrela nodiflora, and grass Digitaria ciliaris. No phytotoxicity symptoms were found, and had a favorable effect on rice plant growth regarding population size and tillers. The yield of rice plants with the highest milled dry grain yield was 4.45 t/ha. [ABSTRACT FROM AUTHOR]

Doubled haploid breeding using anther culture is potentially acclaimed for early fixation of homozygosity. We recovered 129 androgenic doubled haploid lines (DHLs) from a cross Khandagiri (drought sensitive) x Dular (drought tolerant). Fifty five DHLs with high seedling vigour showed wide variability for traits associated with drought tolerance and seed yield under drought stress. DHL 1, DHL 4, DHL 8, DHL 24, DHL 30, DHL 41, DHL 43, DHL 48 and DHL 53 showed higher degree of drought tolerance and also revealed the drought tolerant allele (126 bp) amplified by SSR marker RM 8085. DHL41, DHL 53, DHL43, DHL4 and DHL24 had high yield potential than Sahbhagidhan. However, DHL 41 being a short duration (90 days) and semi-dwarf (101.9 cm) with significantly higher number of grains/panicle, very high 1000-grain weight (28.9 g), fertility percentage (94.2%) and seed yield (46.6 q/ha); can be fitted to upland condition. Besides, the DHLs can serve as an ideal mapping population for mapping and detection of QTLs/genes of interest in upland rice. [ABSTRACT FROM AUTHOR]

Low phosphorus (P) in the upland ecosystems negatively, influence rice growth and causes significant yield losses. In the present study, 9 upland rice genotypes were screened to identify root traits that support the growth in low P soil in a cement tank. Rice genotypes showed significant (p = ≤ 0.05) variation for number of root tips (NRT), number of root branching points (NBP), total root length (TRL), whole root network area (NA), average root diameter, root volume (RV), root surface area (RSA), first order root length (FORL), and second order root length (SORL). BW01 and ITA01 recorded the highest NRT, NBP, TRL, RV, NA, RSA, FORL and SORL while NERICA04 had the lowest representing 5.8, 8.0, 7.6, 6.8, 9.0, 5.8, and 9.3 differences in these traits under low P soil. NRT significantly positively correlated with NBP, TRL, NA, RV, RSA, FORL indicating the role of different root traits in foraging for soil nutrients. The principal component analysis (PCA) showed that the NRT, NBP, TRL, RSA and SORL are important and effective root traits for selection in rice breeding under low P soil supply. BW01 and ITA01 recorded well developed root system indicating that they are P-efficient than P-inefficient NERICA04 under low P soil conditions. Therefore, BW01 and ITA01 can targeted for cultivation in P deficient soils and also used as donor of novel root traits to improve P-inefficient rice cultivars.

Tiller development is regulated by genetics, endogenous hormones, and environmental factors. In this study, rice seedlings from four upland and two lowland rice varieties that were treated with 1.0 mg L− 1 kinetin (a synthetic cytokinin) in vitro could produce additional tillers as early as 20 to 30 days after transplanting, compared to the untreated rice seedlings. However, the number of tillers of kinetin-treated upland rice varieties (Bokilong and Gonsulak) grown in greenhouse did not increase although they developed the first tiller 8 to 9 days earlier than the untreated seedlings. In addition, two lowland rice varieties (Kadaria and MR269) grown in upland growth condition produced 73.7% and 46.5% less tillers, respectively, compared to lowland growth condition; possibly due to an increase of abscisic acid and a lower ratio of trans-zeatin to indole acetic acid. Understanding the effects of kinetin to tiller development in rice may help to increase the total number of tillers and yield of upland rice varieties. [ABSTRACT FROM AUTHOR]

In this study, 10–40% of oat-beta-glucan (OBG), soluble soybean polysaccharide (SSPS), and konjac glucomannan (KGM) were separately supplemented to rice flour (RF) to evaluate the effect of three soluble dietary fibers (SDF) on the cooking properties and eating quality, as well as in vitro digestibility of rice noodles (RNs). The digestive properties results suggested the addition of 20% KGM and 40% SSPS to RF significantly increased resistant starch (RS) and they were a low GI food. Textural properties analysis exhibited the addition of three SDF gave rise to a decrease in hardness (28,883–77,023 Pa), and this hardness met the production standards for RNs. The cooking and sensory quality analysis suggested SSPS had the most significant effect and KGM/OBG had little effect, particularly, 20% KGM improved the water absorption rate of RNs. The chroma results showed KGM had less influence on the L*, a*, and b* of RNs. These indicated that the addition of 20% KGM to RF provided a dietary reference for diabetic patients. [ABSTRACT FROM AUTHOR]

Antioxidant complex enzymes have a significant role in cellular homeostasis control in plants, and they inhibit the toxic action of reactive oxygen species when they are in excess. There are many antioxidant enzymes executing this role; among these, superoxide dismutase, catalase, and ascorbate peroxidase are reported as the most studied in this process, as they prevent free radicals from becoming more reactive and toxic to cells. Thus, this research was conducted to evaluate antioxidant enzyme expression in response to hydric stress at the reproductive stage in upland rice genotypes. Three genotypes from the upland rice breeding program on agreement between UFLA, EPAMIG, and EMBRAPA, CMG2093, CMG2172, and BRSMG Relâmpago, were used as controls. Genotypes were grown under field conditions with supplementary irrigation during the whole crop cycle, and hydric stress was induced in the reproductive phase before panicle emission. Seedlings were used in enzyme analyses from the emergence test and IVE on substrate (soil + sand at a 2:1 rate) at 70% and 10% field capacity. Significant differences were observed among genotypes for vigor tests. In biochemical tests, the CMG2093 genotype had lower damage on hydric deficit, with the best performance under hydric restriction conditions, being considered tolerant for this stress type. [ABSTRACT FROM AUTHOR]

The effects of localized fertilizer (LF) application on changes in root system architecture (RSA) of upland rice seedlings under upland condition were evaluated, and root traits important for deep root development were identified using three root box experiments (Exps). Fertilizer was homogeneously mixed and broadcasted on the soil surface in two control treatments, while in three LF treatments, it was point-placed at 2.5, 7.5 and 12.5 cm below the soil surface. Nitrogen (N) fertilizer in Exp. 1, phosphorus (P) fertilizer in Exp. 2 and compound fertilizer with nitrogen, phosphorus and potassium (NPK) in Exp. 3 were used to compare the effects of LF. Localized N and NPK application altered the RSA through root proliferation and increasing root length percentage around the fertilizer-applied area, whereas localized P application had no effect on both root and shoot development. Deep root and shoot development were decreased with localized NPK application compared to homogeneously mixed control treatment but were maintained with localized N application, when applied at deeper soil depths. Furthermore, the increase in deep root length (DRL) was closely related to the increase in both total root length (TRL) and deep root length ratio (DRR). DRR increased with TRL in Exps. 2 and 3, while it was independent from TRL in Exp. 1, suggesting that DRL can be promoted by increasing DRR regardless of TRL. These results showed that localized N application at deeper soil depths could modify RSA of upland rice seedlings grown under upland condition without affecting shoot growth.

Gene expression can be highly plastic in response to environmental variation. However, we know little about how expression plasticity is shaped by natural selection and evolves in wild and domesticated species. We used genotypic selection analysis to characterize selection on drought‐induced plasticity of over 7,500 leaf transcripts of 118 rice accessions (genotypes) from different environmental conditions grown in a field experiment. Gene expression plasticity was neutral for most gradually plastic transcripts, but transcripts with discrete patterns of expression showed stronger selection on expression plasticity. Whether plasticity was adaptive and co‐gradient or maladaptive and counter‐gradient varied among varietal groups. No transcripts that experienced selection for plasticity across environments showed selection against plasticity within environments, indicating a lack of evidence for costs of adaptive plasticity that may constrain its evolution. Selection on expression plasticity was influenced by degree of plasticity, transcript length and gene body methylation. We observed positive selection on plasticity of co‐expression modules containing transcripts involved in photosynthesis, translation and responsiveness to abiotic stress. Taken together, these results indicate that patterns of selection on expression plasticity were context‐dependent and likely associated with environmental conditions of varietal groups, but that the evolution of adaptive plasticity would likely not be constrained by opposing patterns of selection on plasticity within compared to across environments. These results offer a genome‐wide view of patterns of selection and ecological constraints on gene expression plasticity and provide insights into the interplay between plastic and evolutionary responses to drought at the molecular level. [ABSTRACT FROM AUTHOR]

Over half of the world's arable land is acidic, which constrains cereal production. In South America, different rice-growing regions (Cerrado in Brazil and Llanos in Colombia and Venezuela) are particularly affected due to high aluminum toxicity levels. For this reason, efforts have been made to breed for tolerance to aluminum toxicity using synthetic populations. The breeding program of CIAT-CIRAD is a good example of the use of recurrent selection to increase productivity for the Llanos in Colombia. In this study, we evaluated the performance of genomic prediction models to optimize the breeding scheme by hastening the development of an improved synthetic population and elite lines. We characterized 334 families at the S0:4 generation in two conditions. One condition was the control, managed with liming, while the other had high aluminum toxicity. Four traits were considered: days to flowering (FL), plant height (PH), grain yield (YLD), and zinc concentration in the polished grain (ZN). The population presented a high tolerance to aluminum toxicity, with more than 72% of the families showing a higher yield under aluminum conditions. The performance of the families under the aluminum toxicity condition was predicted using four different models: a single-environment model and three multi-environment models. The multi-environment models differed in the way they integrated genotype-by-environment interactions. The best predictive abilities were achieved using multi-environment models: 0.67 for FL, 0.60 for PH, 0.53 for YLD, and 0.65 for ZN. The gain of multi-environment over single-environment models ranged from 71% for YLD to 430% for FL. The selection of the best-performing families based on multi-trait indices, including the four traits mentioned above, facilitated the identification of suitable families for recombination. This information will be used to develop a new cycle of recurrent selection through genomic selection. [ABSTRACT FROM AUTHOR]

Strong early growth vigor is an essential target in both direct seeded rice breeding and high-yielding rice breeding for rice varieties with relatively short growth duration in the double-cropping region. Shoot dry weight (SDW) is one of the important traits associated with early growth vigor, and breeders have been working to improve this trait. Finding stable QTLs or functional genes for SDW is crucial for improving the early growth vigor by implementing molecular breeding in rice. Here, a genome-wide association analysis revealed that the QTL for SDW, qSDW-5, was stably detected in the three cultivation methods commonly used in production practice. Through gene-based haplotype analysis of the annotated genes within the putative region of qSDW-5, and validated by gene expression and knockout transgenic experiments, LOC_Os05g09520, which is identical to the reported GW5/GSE5 controlling grain width (GW) and thousand grain weight (TGW) was identified as the causal gene for qSDW-5. Five main haplotypes of LOC_Os05g09520 were identified in the diverse international rice collection used in this study and their effects on SDW, GW and TGW were analyzed. Phenotypic comparisons of the major haplotypes of LOC_Os05g09520 in the three subpopulations (indica, japonica and aus) revealed the same patterns of wider GW and higher TGW along with higher SDW. Furtherly, the haplotype analysis of 138 rice varieties/lines widely used in southern China showed that 97.8% of the cultivars/lines carry Hap2LOC_Os05g09520. These results not only provide a promising gene source for the molecular breeding of rice varieties with strong early growth vigor, but also elucidate the effect of the LOC_Os05g09520 haplotypes on SDW, GW, and TGW in rice. Importantly, this study provides direct genetic evidence that these three traits are significantly correlated, and suggests a breeding strategy for developing high-yielding and slender grain-shaped indica cultivars with strong early growth vigor. [ABSTRACT FROM AUTHOR]

Background: Improving the efficiency of nitrogen utilization in rice is vital for achieving significant crop yields with minimum harm to the environment. To tackle this challenge, various portable optical sensors and decision-support tools have been emerging in recent times. However, there is currently a lack of comprehensive assessment and comparison of these tools with different levels of fertilizer recommendation with and without additional supplementation of nano urea. Methods: The current study was carried out during the dry season of 2021 and 2022 at the P.G. Experimental Farm of Centurion University of Technology and Management. The investigated treatments encompassed the following: T1: Absolute control, T2: 90-45-45 (N-P2O5-K2O kg/ha), T3: 120-60-60 (RDF), T4: 150-75-75, T5: T2+NU @ 2 ml/L at panicle initiation, T6: T3+NU @ 2 ml/L at panicle initiation, T7: N application at LCC ≤ 3, T8: N application at LCC ≤ 4, T9: N application at SI < 90%, T10: Nutrient Expert (TY: 5.5 t/ha), T11: Rice crop manager (TY: 5.5t/ha) which were allocated in randomized block design with three replications. Result: The findings showed that implementing nitrogen (N) application when the sufficiency index (SI) is below 90% led to a significant 16.85% boost to grain yield of rice compared to the application of 120-60-60 at fixed intervals as per the standard recommendation. In the former approach, 150 kg N/ha was applied in four splits. However, N application at a leaf color chart (LCC) value of 4 or less was considered efficient, with maximum agronomic efficiency (18.22 and 19.22 kg grain/kg N applied) and recovery efficiency (43.27% and 50.25%) observed in 2021 and 2022, respectively since, this approach resulted in a comparable yield increase and nitrogen absorption with a reduced nitrogen input. [ABSTRACT FROM AUTHOR]

Background: Bacterial leaf blight can damage upland rice and reduce yield by 30-40%. Chemical pesticides harm ecosystems and can increase pathogen resistance. The objective of this research was to explore the potential of AMF, a biological agent, to enhance the resistance of local Acehnese-radiated mutant rice against bacterial leaf blight. Methods: Two-factor factorial design with three levels of AMF dosage (0 g, 10 g and 20 g per plant) and three upland rice lines (UA-01, UA-09 and UA-10). There were 9 treatment combinations replicated 3 times, resulting in 81 experimental units. Result: The intensity of the disease decreased significantly as the dosage of AMF increased and the maximum number of tillers increased substantially up to 20 g plant-1 AMF application. UA-10 strains exhibited the highest number of tillers per clump, while UA-09 strains had the lowest. The interaction between local Aceh rice varieties and AMF did not impact bacterial leaf blight intensity, maximum number of tillers and percentage of filled grain, except for AMF colonization on roots. The significance of AMF colonization varied among lines, with the highest AMF colonization observed in the UA-10 strain (20.64%) at an AMF dose of 20 g plant-1. AMF inoculation appeared to affect the percentage of filled grain positively. [ABSTRACT FROM AUTHOR]

Background: Silicon (Si) has been considered a beneficial element for many plants. Si-rich biochar (Sichars) from rice husk or peanut shell have been suggested to be a potential source of bioavailable Si for Si-accumulator plants. Our study aimed to assess the Si-fertilization efficiency from chemical fertilizer and rice husk and peanut shell biochars on rice dry matter and shoot Si uptake. Methods: Pot trials were conducted in two crop seasons in 2023 (from February to April - spring season and from May to Julysummer season) in the close net house. Treatments included 1 treatment with pure soil (control), 4 treatments with Si and N-P-K fertilizers and 6 treatments with biochar amendments and N-P-K fertilizers. These pot experiments were designed in the randomized complete block (RCBD) with 4 replications. Result: Shoot dry matter increased from 35.6 to 66.7% compared with control in different rates of Si fertilizer application and was also found the highest values in RHB and PSB at 15 g/kg soil application. Different Si and biochar application rates had a significant effect (P<0.05) on Si uptake by rice shoot. Therefore, Si-rich biochar considers as potential material for providing available Si to maintain the required level of available Si in soil and plant for healthy and high crop production. [ABSTRACT FROM AUTHOR]

Rice (Oryza sativa L.) plant architecture is crucial in rice productivity enhancement, particularly in forecasting agronomic-related traits. The presented research characterized the shoot architecture, photosynthesis, and yield-related traits in the F9 generation of rice recombinant inbred lines (RILs) derived from a cross between cultivar IR64 and local cultivar Hawara Bunar through correlation and path analyses among the various features. Rice RILs and parental cultivars’ sowing transpired in the greenhouse and the field. The greenhouse experiment commenced growing the rice seeds of 90 RILs and their parental lines in PVC tubes (20 cm in diameter and 50 cm in height) with sand soil as a medium. The study employed the upland rice cultivation system for the field experiment to cultivate the rice genotypes. The study used a randomized complete block design (RCBD) with three replications. Shoot plant architecture, physiological, and yield traits observation continued at various stages of plant growth. A significant positive correlation to the seed yield per plant appeared in the maturative stage by the total seed weight, transpiration efficiency, intercellular CO2 concentration, and intercellular CO2 pressure. A substantial positive indirect effect on grain yield also surfaced from the net transpiration rate and its efficiency, intercellular CO2 concentration, and CO2 pressure. Therefore, the shoot architecture significantly affects the photosynthetic rate and grain yield. [ABSTRACT FROM AUTHOR]

Drying involves the evaporation of moisture, accompanied by simultaneous heat transfer, mass transfer, and momentum transfer. While the diffusion law is considered an applicable model for explaining the drying phenomenon, the actual drying process cannot be accurately predicted using an analytical solution with a constant diffusion coefficient. Energy efficiency in the drying process is low due to an insufficient understanding of the mechanisms governing moisture migration from solids to air. The development of drying theory has stalled due to an unsolvable discrepancy between experimental results and analytical results. This study analyzes the effect of the binding energy of moisture in paddy rice on the diffusion coefficient. The theoretical relationship between water activity and drying rate in paddy rice was investigated, and the drying process was successfully explained by analyzing free energy transfer and transition theory. The mechanism of rice grain drying was described using a new theoretical solution for the drying process. These results provide new insights into the development of a scientific evaluation standard for assessing the efficiency of actual drying processes. [ABSTRACT FROM AUTHOR]

Simple Summary: Global livestock production systems face feed availability and cost challenges, particularly for high-quality fattening beef cattle. Thus, we require research on grain-rich energy alternatives in their diets. Our study investigated the impact of substituting cassava pulp with broken rice and cassava chips in crossbred Holstein steer diets and the practical implications on the ruminant feeding system. The findings indicate that while broken rice is a superior nutrient compared to cassava pulp and chips, it significantly increased the feed costs. However, substituting cassava pulp and chips positively influenced the ensilage quality and microbial populations without adverse effects on feed intake, enteric methane emissions, and energy utilization. These results suggest that broken rice, an agro-industrial by-product, could be an efficient alternative grain-rich feed resource for ruminants. This study highlights the potential benefits of strategic feeding strategies using broken rice, particularly in tropical cattle production systems. This study evaluates the effects of substituting cassava pulp with broken rice and cassava chips in the total mixed ration silage diets of beef cattle on feed composition, ensiling quality, digestibility, and energy utilization. Fifteen Holstein Thai native crossbred (89% Bos taurus × 11% Bos indicus) steers in the fattening phase, with an average age of 2.5 ± 0.1 years and an initial body weight of 603.7 ± 14.3 kg, were used in the energy balance trial. Using a randomized complete block design with five replications, the steers received one of three treatments. The three dietary treatments included substituting cassava pulp with cassava chips and broken rice on a dry matter basis with ratios of 50:0:0, 30:20:0, or 10:20:20. The results show that broken rice is a superior nutrient source and provides greater energy balance (p < 0.01). Despite the cost implications, substituting cassava pulp and chips positively impacts the ensilage pH and reduces the acetic acid concentration (p < 0.01). There was an increase in the lactic acid bacteria count (p < 0.05) and a reduction in the rumen ammonia, propionate, and butyrate concentrations (p < 0.05) without adverse effects (p > 0.05) on digestibility, blood metabolites, or enteric methane emissions. These findings suggest that broken rice is a promising alternative grain-rich ruminant feed. Future research should explore on-farm long-term feeding and economic evaluations to provide a more comprehensive understanding of the practical implications. [ABSTRACT FROM AUTHOR]

The Experiment was conducted at the Livestock Research Station, Garividi, Vizianagaram District, Andhra Pradesh for 90 days i.e. from July to October, 2023 to assess the impact of incorporating Rice Distillers Dried Grains with Soluble (RDDGS) by substituting soybean meal in the concentrate mixture on the growth, digestibility, and cost economics of Nagavali ram lambs. Twenty-four lambs were randomly assigned into four treatment groups. In the treatment groups, soybean meal was replaced with RDDGS at 0% (T1 ), 50% (T2 ), 75% (T3 ), and 100% (T4 ) levels. Our results inferred that replacing of soyabean meal with different concentrations of RDDGS did not significantly (p>0.05) affect dry matter intake (DMI and feed conversion ratio. Further, gross digestibility of different nutrients and cell wall constituents were also unaffected. The cost of feed kg-1 of weight gain was significantly (p<0.05) reduced. The cost of feed kg-1 gain was significant (p<0.05) because of the replacement of soybean meal with RDDGS. T2, T3 and T4, showing decreases of ₹11.23, ₹17.59, and, respectively, in comparison to the control (T1 ). The study concludes that RDDGS can completely replace soybean meal in sheep diets without affecting nutrient efficiency. This replacement maintains the same growth and digestibility while significantly reducing the cost of production per kilogram of body weight by 12.7%. Consequently, RDDGS emerges as a cost-effective alternative to soybean meal, offering economic benefits while sustaining the nutritional quality of the feed. [ABSTRACT FROM AUTHOR]

Dry direct-seeding can get rid of the constraints of high-water demand, and is the simplest and most laborsaving mode in rice (Oryza sativa L.) cultivation. However, weeds are more prone to occur in dry direct-seeding rice fields, coupled with increased herbicide resistance, leading to confusion among growers regarding herbicide selection. In this study, our investigation results revealed total grass quantity reached 458.82 plants m-2. Two round of field experiments were conducted to determine suitable post-emergence herbicides for dry directseeding indica rice. The results showed that among the herbicides safe to indica rice, the efficacy of the combination of pyraquinate (a new 4-hydroxyphenylpyruvate dioxygenase inhibitor, 150.0 g ai ha-1) + florpyrauxifen-benzyl (13.5 g ai ha-1) on Leptochloa chinensis (L.) Nees, Eclipta prostrata (L.) L., Cyperus iria L., Fimbristylis miliacea auct., and total grasses were 89.1%, 97.8%, 97.2%, 90.1%, and 92.8%, respectively; the efficacy of metamifop (120.0 g ai ha-1) + florpyrauxifen-benzyl (18.0 g ai ha-1) and 22.0% cyhalofop-butyl + pyrazosulfuron-ethyl + bispyribac-sodium OD (148.5 g ai ha-1) on main weeds also exceeds 85.0%, except for cyhalofop-butyl-resistant L. chinensis. The combination of metamifop + florpyrauxifen-benzyl and 22.0% cyhalofop-butyl + pyrazosulfuron-ethyl + bispyribac-sodium OD can be selected for fields where L. chinensis is not resistant to acetyl-CoA carboxylase (ACCase) inhibitors. The combination of pyraquinate + florpyrauxifenbenzyl is the most promising combination to control multiple weeds, even ACCase inhibitors-resistant weeds. This study could also provide references for herbicides selection and management of ACCase inhibitors-resistant L. chinensis in dry direct-seeding indica rice field worldwide. [ABSTRACT FROM AUTHOR]

Background: As a key agricultural region in China, Heilongjiang Province has experienced significant carbon emissions over the past few decades. To understand the underlying factors and future trends in these emissions, a comprehensive analysis was conducted from 1993 to 2030. Methods: The agricultural carbon emissions from 1993 to 2020 were estimated using the emission factor method. To analyze the influencing factors and future trends of these emissions, the study employed the Logarithmic Mean Divisia Index (LMDI) and integrated it with the Stochastic Impacts by Regression on Population, Affluence, and Technology (STIRPAT) model. Results: Results showed that (1) the agricultural carbon emissions in Heilongjiang were primarily driven by rice cultivation, followed by fertilizer production and irrigation electricity. (2) The economic and labor structure effects were the main driving factors of agricultural carbon emissions, while the population, demographic, and intensity effects were the main inhibitors. (3) Agricultural carbon emissions in Heilongjiang Province peaked in 2016 with 69.6 Mt CO2-eq and could subsequently decline by −3.92% to −4.52% between 2020 and 2030 in different scenario simulations. In the future, Heilongjiang Province should prioritize the reduction of agricultural carbon emissions from rice production. Adjusting the planting structure, managing the layout of rice paddies, and promoting the cultivation of dry rice varieties would significantly contribute to mitigating agricultural carbon emissions. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The effects of localized fertilizer (LF) application on changes in root system architecture (RSA) of upland rice seedlings under upland condition were evaluated, and root traits important for deep root development were identified using three root box experiments (Exps). Fertilizer was homogeneously mixed and broadcasted on the soil surface in two control treatments, while in three LF treatments, it was point-placed at 2.5, 7.5 and 12.5 cm below the soil surface. Nitrogen (N) fertilizer in Exp. 1, phosphorus (P) fertilizer in Exp. 2 and compound fertilizer with nitrogen, phosphorus and potassium (NPK) in Exp. 3 were used to compare the effects of LF. Localized N and NPK application altered the RSA through root proliferation and increasing root length percentage around the fertilizer-applied area, whereas localized P application had no effect on both root and shoot development. Deep root and shoot development were decreased with localized NPK application compared to homogeneously mixed control treatment but were maintained with localized N application, when applied at deeper soil depths. Furthermore, the increase in deep root length (DRL) was closely related to the increase in both total root length (TRL) and deep root length ratio (DRR). DRR increased with TRL in Exps. 2 and 3, while it was independent from TRL in Exp. 1, suggesting that DRL can be promoted by increasing DRR regardless of TRL. These results showed that localized N application at deeper soil depths could modify RSA of upland rice seedlings grown under upland condition without affecting shoot growth. [ABSTRACT FROM AUTHOR]

BACKGROUND: Barnyardgrass (Weed Science Society of America recommended) or Barnyard grass (Britannica recommended) (Echinochloa crus‐galli (L.) P. Beauv.) is one of the most problematic and dominant weeds in world agricultural systems, especially in paddy fields, where tillering and grain yield can be reduced by 50–70% because of its competitive pressure. The frequent use of chemical herbicides to control E. crus‐galli has led to the evolution of herbicide resistance. Developing bioherbicides using pathogenic fungi to control E. crus‐galli could be an alternative option. RESULTS: In a previous study we showed that a strain of Bipolaris yamadae (HXDC‐1‐2) was promising in controlling gramineous weeds. Here we present a study that evaluated this fungus as a mycoherbicide against E. crus‐galli in greenhouse and paddy fields, characterized mycelium growth and conidial production, and examined the infection development. The median effective dose (ED50) and 90% effective dose (ED90) values of microcapsulated B. yamadae strain HXDC‐1‐2 on E. crus‐galli in the greenhouse were 7.17 × 102 and 9.35 × 103 conidia mL−1, respectively. Conidial germination, mycelial growth, and attachment formation occurred on E. crus‐galli leaves within 1 to 6 h. The hyphae directly invaded cells and stomata, primarily from the appressorium on the epidermis, and necrotic lesions were observed on the leaf surface within 20 to 24 h. Applied to E. crus‐galli plants at 1 × 105 conidia mL−1, the fungus reduced the weed's fresh weight of 75%. CONCLUSION: B. yamadae strain HXDC‐1‐2 has the potential to be developed as a bioherbicide against E. crus‐galli plants, especially in rice fields. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Background: Production of entomopathogenic fungi on a solid substrate, mostly on rice, is preferred by many producers due to its low technological requirements. Drying may be needed after fermentation to preserve the viability of the conidia until use. This study aimed to introduce some uncomplicated drying techniques and study their effectiveness in terms of drying time and extent, as well as their impact on the quantity of conidia produced and their biological activity. Four techniques were studied, i.e., condensing water vapor (by cooling), desiccant (using NaOH), air drying on a net, and air drying on a solid (impermeable) surface. Results: Condensing water vapor and desiccant techniques were the fastest and most efficient, resulting in the lowest moisture content, ranging between 12 and 13%. While drying on a solid surface was the slowest and resulted in the highest moisture content of 32.98%. All of the dried samples yielded a lower quantity of conidia compared to the fresh samples. Samples of condensing water vapor, desiccant, and net techniques yielded approximately a similar quantity of conidia, ranging between 74 and 71% of the yield of the fresh sample. The solid surface sample yielded the lowest quantity of conidia, representing 63.78% of the fresh sample yield. A bioassay test on the mite, Tetranychus urticae Koch (Acari: Tetranychidae), showed that conidia dried via condensing water vapor and desiccant techniques kept their pathogenicity similar to the fresh sample, while samples dried on a net and solid surface significantly lost part of their pathogenicity. Conclusion: The condensing water vapor and desiccant techniques were the most efficient regarding the time needed, conidia yield, and pathogenicity. Drying on a solid surface was the least effective regarding the same factors. [ABSTRACT FROM AUTHOR]

The rice waterlogging stress test was conducted at the experimental base of the College of Agriculture, Yangtze University, using Yangxian You 418 as the test subject, in order to investigate the impact of waterlogging on rice growth during the period from July to August each year. Six waterlogging stress tests with different waterlogging depth (1/4 plant height (1/4PH), 2/4 PH, and 3/4 PH) and duration (5 d and 7 d) were set up at the jointing–booting stage of rice (T1: 1/4 PH, 7 d; T2: 2/4 PH, 7 d; T3: 3/4 PH, 7 d; T4: 1/4 PH, 5 d; T5: 2/4 PH, 5 d; T6: 3/4 PH, 5 d;) with shallow water irrigation (CK) as control. The plant height, population leaf area, above-ground dry matter, and the yield of rice were measured. The correlation between the waterlogging depth and rice yield reduction was analyzed, and the flood disaster threshold index of rice was established. The results showed that at the end of stress, the plant height of all waterlogged treatments exceeded CK, and the plant height of T3 and T6 treatments significantly increased by 31.90% and 15.93%, respectively. The leaf area of rice treated with T1, T3, T4, and T5 was higher than CK (p < 0.05), and the above-ground dry matter of rice treated with T2, T3, T4, T5, and T6 was higher than CK (p < 0.05). When normal irrigation was restored to the maturity stage, the plant height of all rice treated with waterlogging was still higher than CK (p < 0.05). However, as the degree of waterlogging increased, rice yield decreased significantly, with a notable reduction of 31.68% observed in the T3 treatment compared to CK. Assuming a drainage index based on a 20% decrease in rice yield, it is imperative that the ratio of flooded depth to plant height remains below 37% when waterlogging persists for 7 days in rice cultivation. These research findings offer crucial scientific insights for implementing effective drainage management measures during flood disasters in rice paddies. [ABSTRACT FROM AUTHOR]

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