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1. Inflammation of the nasal mucosa is associated with susceptibility to experimental pneumococcal challenge in older adults

Author

Urban, Britta C., Gonçalves, André N.A., Loukov, Dessi, Passos, Fernando M., Reiné, Jesús, Gonzalez-Dias, Patrícia, Solórzano, Carla, Mitsi, Elena, Nikolaou, Elissavet, O’Connor, Daniel, Collins, Andrea M., Adler, Hugh, Pollard, Andrew, Rylance, Jamie, Gordon, Stephen B., Jochems, Simon P., Nakaya, Helder I., and Ferreira, Daniela M.

Published
2024
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2. Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity

Author

Hoving, Dennis, Marques, Alexandre H. C., Huisman, Wesley, Nosoh, Beckley A., de Kroon, Alicia C., van Hengel, Oscar R. J., Wu, Bing-Ru, Steenbergen, Rosanne A. M., van Helden, Pauline M., Urban, Britta C., Dhar, Nisha, Ferreira, Daniela M., Kwatra, Gaurav, Hokke, Cornelis H., and Jochems, Simon P.

Published
2023
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3. Respiratory mucosal immune memory to SARS-CoV-2 after infection and vaccination

Author

Mitsi, Elena, Diniz, Mariana O., Reiné, Jesús, Collins, Andrea M., Robinson, Ryan E., Hyder-Wright, Angela, Farrar, Madlen, Liatsikos, Konstantinos, Hamilton, Josh, Onyema, Onyia, Urban, Britta C., Solórzano, Carla, Belij-Rammerstorfer, Sandra, Sheehan, Emma, Lambe, Teresa, Draper, Simon J., Weiskopf, Daniela, Sette, Alessandro, Maini, Mala K., and Ferreira, Daniela M.

Published
2023
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4. Whole blood versus red cell concentrates for children with severe anaemia: a secondary analysis of the Transfusion and Treatment of African Children (TRACT) trial

Author

Maitland, Kathryn, Walker, Sarah A, George, Elizabeth C, Williams, Thomas N, Gibb, Diana M, Mpoya, Ayub, Frost, Gary, Walsh, Kevin, Olupot-Olupot, Peter, Nteziyaremye, Julius, Namayanja, Cate, Ssenyondo, Tonny, Passi, George, Muhindo, Rita, Masifa, George, Adong, Ruth, Okalebo, Charles B, Atimango, Emmanuela, Thembo, Nobert, Odong, George, Kiluli, Godfrey, Kapsindet, Job, Kiguli, Sarah, Opoka, Robert O, Kayaga, Julianne, Nabawanuka, Eva, Kadama, Eva, Mukisa, Cynthia Williams, Engoru, Charles, Alaroker, Florence, Nakuya, Margaret, Amorut, Denis, Olupot, Moses, Onyas, Pius, Ariimi, Margaret, Itipe, Melda, Atim, Mary G, Abeno, Mary, Okwi, Steven, Kulume, Mary G, Among, Grace, Achipa, Dorreen E, Uyoga, Sophie, Macharia, Alex, Mellewa, Machpherson, Chagaluka, George, Kennedy, Neil, Kumwenda, Felistas, Sochera, Tiferanji Fatch, Malenga, Albert, Chimalizeni, Yamikani FG, Gushu, Benard, Phiri, Tusekile, Chesale, Amisa, Mitole, Ndaona, Chokani, Ellida, Munthali, Annie, Hensbroek, Michael Boele von, South, Annabelle, Thomason, Margaret J, Baptiste, David, Connon, Roisin, MacCabe, Leanne, Ali, Abdul, Khamis, Kibibi, Madoola, Macreen, Abongo, Grace, Bates, Imelda, Urban, Britta, Heydermann, Robert, Kyomuhendo, Flavia, Nakalanzi, Sarah, Chabuka, John, Mkandawire, Nkhafwire, Fitzgerald, Felicity, Evans, Jennifer A, Molyneux, Elizabeth, Lubega, Irene, Crawley, Jane, Kazembe, Peter, Murphy, Mike, Peto, Tim, Todd, Jim, Mirembe, Grace, Musoka, Philipa, Tenu, Filemoni, M'baya, Bridon, Kyeyune Byabazair, Dorothy, Mbanya, Dora, and Walker, A Sarah

Published
2022
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5. Effect of co-infection with intestinal parasites on COVID-19 severity: A prospective observational cohort study

Author

Wolday, Dawit, Gebrecherkos, Teklay, Arefaine, Zekarias Gessesse, Kiros, Yazezew Kebede, Gebreegzabher, Atsbeha, Tasew, Geremew, Abdulkader, Mahmud, Abraha, Hiluf Ebuy, Desta, Abraham Aregay, Hailu, Ataklti, Tollera, Getachew, Abdella, Saro, Tesema, Masresha, Abate, Ebba, Endarge, Kidist Lakew, Hundie, Tsegaye Gebreyes, Miteku, Frehiwot Kassahun, Urban, Britta C., Schallig, Henk H.D.F., Harris, Vanessa C., and de Wit, Tobias F. Rinke

Published
2021
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6. Serotype 3 Experimental Human Pneumococcal Challenge (EHPC) study protocol: dose ranging and reproducibility in a healthy volunteer population (challenge 3)

Author

Hazenberg, Phoebe, primary, Robinson, Ryan E, additional, Farrar, Madlen, additional, Solorzano, Carla, additional, Hyder-Wright, Angela, additional, Liatsikos, Konstantinos, additional, Brunning, Jaye, additional, Fleet, Hannah, additional, Bettam, Amy, additional, Howard, Ashleigh, additional, Kenny-Nyazika, Tinashe, additional, Urban, Britta, additional, Mitsi, Elena, additional, El Safadi, Dima, additional, Davies, Kelly, additional, Lesosky, Maia, additional, Gordon, Stephen B, additional, Ferreira, Daniela M, additional, and Collins, Andrea M, additional

Published
2024
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7. Cellular and Transcriptional Signature of the Nasal Mucosa is Associated with Susceptibility to Pneumococcal Carriage in Older Adults

Author

Urban, Britta C, primary, Goncalves, Andre NA, additional, Loukov, Dessi, additional, Passos, Fernando M, additional, Reine, Jesus, additional, Gonzalez-Dias, Patricia, additional, Solorzano-Gonzalez, Carla, additional, Mitsi, Elena, additional, Nikolaou, Elissavet, additional, O'Connor, Daniel, additional, Collins, Andrea M, additional, Adler, Hugh, additional, Rylance, Jamie, additional, Gordon, Stephen B, additional, Jochems, Simon P, additional, Nakaya, Helder I, additional, and Ferreira, Daniela M, additional

Published
2023
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8. Participant perceptions and experiences of a novel community-based respiratory longitudinal sampling method in Liverpool, UK: A mixed methods feasibility study

Author

German, Esther L., primary, Nabwera, Helen M., additional, Robinson, Ryan, additional, Shiham, Farah, additional, Liatsikos, Kostas, additional, Parry, Christopher M., additional, McNamara, Claire, additional, Kattera, Sanjana, additional, Carter, Katie, additional, Howard, Ashleigh, additional, Pojar, Sherin, additional, Hamilton, Joshua, additional, Matope, Agnes, additional, Read, Jonathan M., additional, Allen, Stephen J., additional, Hill, Helen, additional, Hawcutt, Daniel B., additional, Urban, Britta C., additional, Collins, Andrea M., additional, Ferreira, Daniela M., additional, and Nikolaou, Elissavet, additional

Published
2023
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9. Erratum: Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Author

Jochems, Simon P., de Ruiter, Karin, Solorzano, Carla, Voskamp, Astrid, Mitsi, Elena, Nikolaou, Elissavet, Carniel, Beatriz F., Pojar, Sherin, German, Esther L., Reine, Jesus, Soares-Schanoski, Alessandra, Hill, Helen, Robinson, Rachel, Hyder-Wright, Angela D., Weight, Caroline M., Durrenberger, Pascal F., Heyderman, Robert S., Gordon, Stephen B., Smits, Hermelijn H., Urban, Britta C., Rylance, Jamie, Collins, Andrea M., Wilkie, Mark D., Lazarova, Lepa, Leong, Samuel C., Yazdanbakhsh, Maria, and Ferreira, Daniela M.

Subjects
Health care industry
Abstract

Original citation: J Clin Invest. 2019;129(10):4523-4538. https://doi.org/10.1172/JCI128865. Citation for this erratum: J Clin Invest. 2022;132(11):e161565. https://doi.org/10.1172/JCI161565. During the preparation of this manuscript, the labels for Figure 5, A and D, [...]

Published
2022
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10. The global transcriptome of Plasmodium falciparum mid-stage gametocytes (stages II–IV) appears largely conserved and gametocyte-specific gene expression patterns vary in clinical isolates

Author

Kengne-Ouafo, Jonas A., primary, Bah, Saikou Y., additional, Kemp, Alison, additional, Stewart, Lindsay, additional, Amenga-Etego, Lucas, additional, Deitsch, Kirk W., additional, Rayner, Julian C., additional, Billker, Oliver, additional, Binka, Fred N., additional, Sutherland, Colin J., additional, Awandare, Gordon A., additional, Urban, Britta C., additional, and Dinko, Bismarck, additional

Published
2023
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11. SARS-CoV-2 antibody response among COVID-19 patients is not affected by parasite co-infection

Author

Gebrecherkos, Teklay, primary, Kebede, Yazezew, additional, Challa, Feyissa, additional, Gebreegzabher, Atsbeha, additional, Abdella, Saro, additional, Leta, Dereje, additional, Desta, Abraham, additional, Hailu, Ataklti, additional, Tasew, Geremew, additional, Abdulkadir, Mahmud, additional, Tessema, Masresha, additional, Tollera, Getachew, additional, Gessesse, Zekarias, additional, DF Schallig, Henk H, additional, Urban, Britta C., additional, Rinke de Wit, Tobias, additional, and Welday, Dawit, additional

Published
2023
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12. Streptococcus pneumoniae colonization associates with impaired adaptive immune responses against SARS-CoV-2

Author

Mitsi, Elena, Reine, Jesus, Urban, Britta C., Solorzano, Carla, Nikolaou, Elissavet, Hyder-Wright, Angela D., Pojar, Sherin, Howard, Ashleigh, Hitchins, Lisa, Glynn, Sharon, Farrar, Madlen C., Liatsikos, Konstantinos, Collins, Andrea M., Walker, Naomi F., Hill, Helen C., German, Esther L., Cheliotis, Katerina S., Byrne, Rachel L., Williams, Christopher T., Cubas-Atienzar, Ana I., Fletcher, Tom E., Adams, Emily R., Draper, Simon J., Pulido, David, Beavon, Rohini, Theilacker, Christian, Begier, Elizabeth, Jodar, Luis, Gessner, Bradford D., and Ferreira, Daniela M.

Subjects
Streptococcus pneumoniae -- Physiological aspects, Immunological research, Immune response -- Health aspects, Host-bacteria relationships -- Research, Streptococcal infections -- Complications and side effects, Health care industry
Abstract

BACKGROUND. Although recent epidemiological data suggest that pneumococci may contribute to the risk of SARS-CoV-2 disease, cases of coinfection with Streptococcus pneumoniae in patients with coronavirus disease 2019 (COVID-19) during hospitalization have been reported infrequently. This apparent contradiction may be explained by interactions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and pneumococci in the upper airway, resulting in the escape of SARS-CoV-2 from protective host immune responses. METHODS. Here, we investigated the relationship of these 2 respiratory pathogens in 2 distinct cohorts of health care workers with asymptomatic or mildly symptomatic SARS-CoV-2 infection identified by systematic screening and patients with moderate to severe disease who presented to the hospital. We assessed the effect of coinfection on host antibody, cellular, and inflammatory responses to the virus. RESULTS. In both cohorts, pneumococcal colonization was associated with diminished antiviral immune responses, which primarily affected mucosal IgA levels among individuals with mild or asymptomatic infection and cellular memory responses in infected patients. CONCLUSION. Our findings suggest that S. pneumoniae impair host immunity to SARS-CoV-2 and raise the question of whether pneumococcal carriage also enables immune escape of other respiratory viruses and facilitates reinfection. TRIAL REGISTRATION. ISRCTN89159899 (FASTER study) and ClinicalTrials.gov NCT03502291 (LAIV study)., Introduction Despite the widespread global effects of the coronavirus disease 2019 (COVID-19) pandemic, few reports have assessed potential interactions between upper airway bacterial colonization and severe acute respiratory syndrome coronavirus [...]

Published
2022
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13. Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Author

Jochems, Simon P., de Ruiter, Karin, Solorzano, Carla, Voskamp, Astrid, Mitsi, Elena, Nikolaou, Elissavet, Carniel, Beatriz F., Pojar, Sherin, German, Esther L., Reine, Jesus, Soares-Schanoski, Alessandra, Hill, Helen, Robinson, Rachel, Hyder-Wright, Angela D., Weight, Caroline M., Durrenberger, Pascal F., Heyderman, Robert S., Gordon, Stephen B., Smits, Hermelijn H., Urban, Britta C., Rylance, Jamie, Collins, Andrea M., Wilkie, Mark D., Lazarova, Lepa, Leong, Samuel C., Yazdanbakhsh, Maria, and Ferreira, Daniela M.

Subjects
Thermo Fisher Scientific Inc., Polysaccharides, B cells, Pneumonia, Immune response, Scientific equipment industry, T cells, Lung diseases, Infection, Streptococcus pneumoniae, Respiratory tract diseases, Health care industry
Abstract

Streptococcus pneumoniae (Spn) is a common cause of respiratory infection, but also frequently colonizes the nasopharynx in the absence of disease. We used mass cytometry to study immune cells from nasal biopsy samples collected following experimental human pneumococcal challenge in order to identify immunological mechanisms of control of Spn colonization. Using 37 markers, we characterized 293 nasal immune cell clusters, of which 7 were associated with Spn colonization. B cell and [CD161.sup.+][CD8.sup.+] T cell clusters were significantly lower in colonized than in noncolonized subjects. By following a second cohort before and after pneumococcal challenge we observed that B cells were depleted from the nasal mucosa upon Spn colonization. This associated with an expansion of Spn polysaccharide- specific and total plasmablasts in blood. Moreover, increased responses of blood mucosa-associated invariant T (MAIT) cells against in vitro stimulation with pneumococcus prior to challenge associated with protection against establishment of Spn colonization and with increased mucosal MAIT cell populations. These results implicate MAIT cells in the protection against pneumococcal colonization and demonstrate that colonization affects mucosal and circulating B cell populations., Introduction Streptococcus pneumoniae (Spn) is a major cause of morbidity and mortality worldwide (1, 2). It is the most common bacterial cause of otitis media, pneumonia, and meningitis in children [...]

Published
2019
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14. Participant perceptions and experiences of a novel community-based pilot respiratory longitudinal sampling method in Liverpool, UK

Author

German, Esther Lauryn, primary, Nabwera, Helen M, additional, Robinson, Ryan, additional, Shiham, Farah, additional, Liatsikos, Kostas, additional, Parry, Christopher M, additional, McNamara, Claire, additional, Kattera, Sanjana, additional, Carter, Katie, additional, Howard, Ashleigh, additional, Pojar, Sherin, additional, Hamilton, Josh, additional, Matope, Agnes, additional, Read, Jonathan M, additional, Allen, Stephen J, additional, Hill, Helen, additional, Hawcutt, Daniel B, additional, Urban, Britta C, additional, Collins, Andrea M, additional, Ferreira, Daniela M, additional, and Nikolaou, Elissavet, additional

Published
2023
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15. Prognostic Value of C-Reactive Protein in SARS-CoV-2 Infection: A Simplified Biomarker of COVID-19 Severity in Northern Ethiopia

Author

Gebrecherkos, Teklay, primary, Challa, Feyissa, additional, Tasew, Geremew, additional, Gessesse, Zekarias, additional, Kiros, Yazezew, additional, Gebreegziabxier, Atsbeha, additional, Abdulkader, Mahmud, additional, Desta, Abraham Aregay, additional, Atsbaha, Ataklti Hailu, additional, Tollera, Getachew, additional, Abrahim, Saro, additional, Urban, Britta C, additional, Schallig, Henk, additional, Rinke de Wit, Tobias, additional, and Wolday, Dawit, additional

Published
2023
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17. Family Research of Microbes Linked to Respiratory Infections (FAMILY Micro) observational study: Assessing the use of minimally invasive self-sampling methodologies at home for long-term monitoring of the oral, nasal and hand microbiota of adults and children within UK families

Author

Nikolaou, Elissavet, primary, German, Esther Lauryn, additional, Howard, Ashleigh, additional, Nabwera, Helen M, additional, Matope, Agnes, additional, Robinson, Ryan, additional, Shiham, Farah, additional, Liatsikos, Kostas, additional, McNamara, Claire, additional, Kattera, Sanjana, additional, Carter, Katie, additional, Parry, Christopher M, additional, Read, Jonathan M, additional, Allen, Stephen J, additional, Urban, Britta C, additional, Hawcutt, Dan B, additional, Hill, Helen, additional, Collins, Andrea M, additional, and Ferreira, Daniela M, additional

Published
2023
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18. Prognostic Value of C-Reactive Protein in SARS-CoV-2 Infection: A Simplified Biomarker of COVID-19 Severity in Northern Ethiopia

Author

Gebrecherkos,Teklay, Challa,Feyissa, Tasew,Geremew, Gessesse,Zekarias, Kiros,Yazezew, Gebreegziabxier,Atsbeha, Abdulkader,Mahmud, Desta,Abraham Aregay, Atsbaha,Ataklti Hailu, Tollera,Getachew, Abrahim,Saro, Urban,Britta C, Schallig,Henk, Rinke de Wit,Tobias, Wolday,Dawit, Gebrecherkos,Teklay, Challa,Feyissa, Tasew,Geremew, Gessesse,Zekarias, Kiros,Yazezew, Gebreegziabxier,Atsbeha, Abdulkader,Mahmud, Desta,Abraham Aregay, Atsbaha,Ataklti Hailu, Tollera,Getachew, Abrahim,Saro, Urban,Britta C, Schallig,Henk, Rinke de Wit,Tobias, and Wolday,Dawit

Abstract

Teklay Gebrecherkos,1 Feyissa Challa,2 Geremew Tasew,3 Zekarias Gessesse,4 Yazezew Kiros,4 Atsbeha Gebreegziabxier,5 Mahmud Abdulkader,1 Abraham Aregay Desta,6 Ataklti Hailu Atsbaha,7 Getachew Tollera,8 Saro Abrahim,5 Britta C Urban,9 Henk Schallig,10 Tobias Rinke de Wit,11,12 Dawit Wolday1,5 1Department of Medical Microbiology and Immunology, College of Health Sciences (CHS), Mekelle University (MU), Mekelle, Tigray, Ethiopia; 2National Reference Laboratory for Clinical Chemistry, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 3Department of Bacteriology, Parasitology and Zoonosis, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 4Department of Internal Medicine, College of Health Sciences, Mekelle University, Mekelle, Tigray, Ethiopia; 5HIV/TB Research Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 6Public Health Research and Emergency Management, Tigray Health Research Institute, Mekelle, Tigray, Ethiopia; 7Department of Microbiology, Tigray Health Research Institute, Mekelle, Tigray, Ethiopia; 8Research and Technology Transfer Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 9Department of Clinical Sciences, Respiratory Clinical Research Group, Liverpool School of Tropical Medicine, Liverpool, UK; 10Department of Medical Microbiology and Infection Prevention, Experimental Parasitology Unit, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; 11Amsterdam Institute of Global Health and Development, Department of Global Health, Amsterdam University Medical Center, Amsterdam, the Netherlands; 12Joep-Lange Institute, Amsterdam, the NetherlandsCorrespondence: Teklay Gebrecherkos, Department of Medical Microbiology and Immunology, Mekelle University, P.O. Box 1871, Mekelle, Tigray, Ethiopia, Email estiftg17@gmail.comPurpose: To evaluate the role of C-reactive protein (CRP) in predicting severe COVID-19 patients.Methods: A prospective observational cohort study was conducted from Jul

Published
2023

19. Participant perceptions and experiences of a novel community-based respiratory longitudinal sampling method in Liverpool, UK : A mixed methods feasibility study

Author

Umer, Muhammad Farooq, German, Esther L., Nabwera, Helen M., Robinson, Ryan, Shiham, Farah, Liatsikos, Kostas, Parry, Christopher M., McNamara, Claire, Kattera, Sanjana, Carter, Katie, Howard, Ashleigh, Pojar, Sherin, Hamilton, Joshua, Matope, Agnes, Read, Jonathan M., Allen, Stephen J., Hill, Helen, Hawcutt, Daniel B., Urban, Britta C., Collins, Andrea M., Ferreira, Daniela M., Nikolaou, Elissavet, Umer, Muhammad Farooq, German, Esther L., Nabwera, Helen M., Robinson, Ryan, Shiham, Farah, Liatsikos, Kostas, Parry, Christopher M., McNamara, Claire, Kattera, Sanjana, Carter, Katie, Howard, Ashleigh, Pojar, Sherin, Hamilton, Joshua, Matope, Agnes, Read, Jonathan M., Allen, Stephen J., Hill, Helen, Hawcutt, Daniel B., Urban, Britta C., Collins, Andrea M., Ferreira, Daniela M., and Nikolaou, Elissavet

Abstract

Longitudinal, community-based sampling is important for understanding prevalence and transmission of respiratory pathogens. Using a minimally invasive sampling method, the FAMILY Micro study monitored the oral, nasal and hand microbiota of families for 6 months. Here, we explore participant experiences and opinions. A mixed methods approach was utilised. A quantitative questionnaire was completed after every sampling timepoint to report levels of discomfort and pain, as well as time taken to collect samples. Participants were also invited to discuss their experiences in a qualitative structured exit interview. We received questionnaires from 36 families. Most adults and children >5y experienced no pain (94% and 70%) and little discomfort (73% and 47% no discomfort) regardless of sample type, whereas children ≤5y experienced variable levels of pain and discomfort (48% no pain but 14% hurts even more, whole lot or worst; 38% no discomfort but 33% moderate, severe, or extreme discomfort). The time taken for saliva and hand sampling decreased over the study. We conducted interviews with 24 families. Families found the sampling method straightforward, and adults and children >5y preferred nasal sampling using a synthetic absorptive matrix over nasopharyngeal swabs. It remained challenging for families to fit sampling into their busy schedules. Adequate fridge/freezer space and regular sample pick-ups were found to be important factors for feasibility. Messaging apps proved extremely effective for engaging with participants. Our findings provide key information to inform the design of future studies, specifically that self-sampling at home using minimally invasive procedures is feasible in a family context.

Published
2023

20. The global transcriptome of Plasmodium falciparum midstage gametocytes (stages II–IV) appears largely conserved and gametocyte-specific gene expression patterns vary in clinical isolates

Author

Kengne-Ouafo, Jonas A., Bah, Saikou Y., Kemp, Alison, Stewart, Lindsay, Amenga-Etego, Lucas, Deitsch, Kirk W., Rayner, Julian C., Billker, Oliver, Binka, Fred N., Sutherland, Colin J., Awandare, Gordon A., Urban, Britta C., Dinko, Bismarck, Kengne-Ouafo, Jonas A., Bah, Saikou Y., Kemp, Alison, Stewart, Lindsay, Amenga-Etego, Lucas, Deitsch, Kirk W., Rayner, Julian C., Billker, Oliver, Binka, Fred N., Sutherland, Colin J., Awandare, Gordon A., Urban, Britta C., and Dinko, Bismarck

Abstract

Our overall understanding of the developmental biology of malaria parasites has been greatly enhanced by recent advances in transcriptomic analysis. However, most of these investigations rely on laboratory strains (LS) that were adapted into in vitro culture many years ago, and the transcriptomes of clinical isolates (CI) circulating in human populations have not been assessed. In this study, RNA-seq was used to compare the global transcriptome of mid-stage gametocytes derived from three short-term cultured CI, with gametocytes derived from the NF54 reference laboratory strain. The core transcriptome appeared to be consistent between CI- and LS-derived gametocyte preparations, but some important differences were also observed. A majority of gametocyte-specific genes (43/53) appear to have relatively higher expression in CI-derived gametocytes than in LS-derived gametocytes, but a K-means clustering analysis showed that genes involved in flagellum- and microtubule-based processes (movement/motility) were more abundant in both groups, albeit with some differences between them. In addition, gametocytes from one CI described as CI group II gametocytes (CI:GGII) showed gene expression variation in the form of reduced gametocyte-specific gene expression compared to the other two CI-derived gametocytes (CI gametocyte group I, CI:GGI), although the mixed developmental stages used in our study is a potential confounder, only partially mitigated by the inclusion of multiple replicates for each CI. Overall, our study suggests that there may be subtle differences in the gene expression profiles of mid-stage gametocytes from CI relative to the NF54 reference strain of Plasmodium falciparum. Thus, it is necessary to deploy gametocyte-producing clinical parasite isolates to fully understand the diversity of gene expression strategies that may occur during the sequestered development of parasite sexual stages. IMPORTANCE Maturing gametocytes of Plasmodium falciparum are known to se

Published
2023
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22. Long-term respiratory mucosal immune memory to SARS-CoV-2 after infection and vaccination

Author

Mitsi, Elena, primary, Diniz, Mariana, additional, Reine, Jesus, additional, Collins, Andrea, additional, Robinson, Ryan, additional, Hyder-Wright, Angela, additional, Farrar, Madlen, additional, Liatsikos, Konstantinos, additional, Hamilton, Josh, additional, Onyema, Onyia, additional, Urban, Britta, additional, Solorzano, Carla, additional, Lambe, Teresa, additional, Draper, Simon, additional, Weiskopf, Daniela, additional, Sette, Alessandro, additional, Maini, Mala, additional, and Ferreira, Daniela, additional

Published
2023
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23. Pharmacokinetics and pharmacodynamics of azithromycin in severe malaria bacterial co-infection in African children (TABS-PKPD): a protocol for a Phase II randomised controlled trial

Author

Olupot-Olupot, Peter, primary, Okiror, William, additional, Mnjalla, Hellen, additional, Muhindo, Rita, additional, Uyoga, Sophie, additional, Mpoya, Ayub, additional, Williams, Thomas N, additional, terHeine, Rob, additional, Burger, David M, additional, Urban, Britta, additional, Connon, Roisin, additional, George, Elizabeth C, additional, Gibb, Diana M, additional, Walker, A Sarah, additional, and Maitland, Kathryn, additional

Published
2023
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28. Protocol for a phase IV double-blind randomised controlled trial to investigate the effect of the 13-valent pneumococcal conjugate vaccine and the 23-valent pneumococcal polysaccharide vaccine on pneumococcal colonisation using the experimental human pneumococcal challenge model in healthy adults (PREVENTING PNEUMO 2)

Author

Liatsikos, Konstantinos, primary, Hyder-Wright, Angela, additional, Pojar, Sherin, additional, Chen, Tao, additional, Wang, Duolao, additional, Davies, Kelly, additional, Myerscough, Christopher, additional, Reine, Jesus, additional, Robinson, Ryan E, additional, Urban, Britta, additional, Mitsi, Elena, additional, Solorzano, Carla, additional, Gordon, Stephen B, additional, Quinn, Angela, additional, Pan, Kaijie, additional, Anderson, Annaliesa S, additional, Theilacker, Christian, additional, Begier, Elizabeth, additional, Gessner, Bradford D, additional, Collins, Andrea, additional, and Ferreira, Daniela M, additional

Published
2022
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29. Innate and adaptive nasal mucosal immune responses following experimental human pneumococcal colonization

Author

Jochems, Simon P., primary, de Ruiter, Karin, additional, Solórzano, Carla, additional, Voskamp, Astrid, additional, Mitsi, Elena, additional, Nikolaou, Elissavet, additional, Carniel, Beatriz F., additional, Pojar, Sherin, additional, German, Esther L., additional, Reiné, Jesús, additional, Soares-Schanoski, Alessandra, additional, Hill, Helen, additional, Robinson, Rachel, additional, Hyder-Wright, Angela D., additional, Weight, Caroline M., additional, Durrenberger, Pascal F., additional, Heyderman, Robert S., additional, Gordon, Stephen B., additional, Smits, Hermelijn H., additional, Urban, Britta C., additional, Rylance, Jamie, additional, Collins, Andrea M., additional, Wilkie, Mark D., additional, Lazarova, Lepa, additional, Leong, Samuel C., additional, Yazdanbakhsh, Maria, additional, and Ferreira, Daniela M., additional

Published
2022
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30. Influence of sex, season and environmental air quality on experimental human pneumococcal carriage acquisition:a retrospective cohort analysis

Author

Cheliotis, Katerina S, Jewell, Christopher P, Solórzano, Carla, Urban, Britta, Collins, Andrea M, Mitsi, Elena, Pojar, Sherin, Nikolaou, Elissavet, German, Esther L, Reiné, Jesús, Gordon, Stephen B, Jochems, Simon P, Rylance, Jamie, Ferreira, Daniela M, Cheliotis, Katerina S, Jewell, Christopher P, Solórzano, Carla, Urban, Britta, Collins, Andrea M, Mitsi, Elena, Pojar, Sherin, Nikolaou, Elissavet, German, Esther L, Reiné, Jesús, Gordon, Stephen B, Jochems, Simon P, Rylance, Jamie, and Ferreira, Daniela M

Abstract

(pneumococcus) is the most commonly identified bacterial cause of pneumonia and the leading infectious cause of death in children under 5 years of age worldwide. Pneumococcal disease follows a seasonal pattern with increased incidence during winter. Pneumonia burden is also associated with poor air quality. Nasopharyngeal carriage of the bacterium is a pre-requisite of invasive disease. We aimed to determine if susceptibility to nasopharyngeal pneumococcal carriage varied by season and which environmental factors might explain such variation. We also evaluated the influence of sex on susceptibility of carriage. We collated data from five studies in which human volunteers underwent intranasal pneumococcal challenge. Generalised linear mixed-effects models were used to identify factors associated with altered risk of carriage acquisition, specifically climate and air-quality data. During 2011-2017, 374 healthy adults were challenged with type 6B pneumococcus. Odds of carriage were significantly lower in males (OR, 0.61; 95% CI, 0.40-0.92; p=0.02), and higher with cooler temperatures (OR, 0.79; 95% CI, 0.63-0.99; p=0.04). Likelihood of carriage was also associated with lower concentrations of local fine particulate matter concentrations (PM ) and increased local rainfall. In contrast to epidemiological series, experimental challenge allowed us to test propensity to acquisition during controlled exposures; immunological explanations for sex and climatic differences should be sought.

Published
2022

32. Influence of sex, season and environmental air quality on experimental human pneumococcal carriage acquisition: a retrospective cohort analysis

Author

Cheliotis, Katerina S., primary, Jewell, Christopher P., additional, Solórzano, Carla, additional, Urban, Britta, additional, Collins, Andrea M., additional, Mitsi, Elena, additional, Pojar, Sherin, additional, Nikolaou, Elissavet, additional, German, Esther L., additional, Reiné, Jesús, additional, Gordon, Stephen B., additional, Jochems, Simon P., additional, Rylance, Jamie, additional, and Ferreira, Daniela M., additional

Published
2022
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33. Longitudinal profile of antibody response to SARS-CoV-2 in patients with COVID-19 in a setting from Sub–Saharan Africa: A prospective longitudinal study

Author

Gebrecherkos, Teklay, primary, Kiros, Yazezew Kebede, additional, Challa, Feyissa, additional, Abdella, Saro, additional, Gebreegzabher, Atsbeha, additional, Leta, Dereje, additional, Desta, Abraham, additional, Hailu, Ataklti, additional, Tasew, Geremew, additional, Abdulkader, Mahmud, additional, Tessema, Masresha, additional, Tollera, Getachew, additional, Kifle, Tsigereda, additional, Arefaine, Zekarias Gessesse, additional, Schallig, Henk HDF, additional, Adams, Emily R., additional, Urban, Britta C., additional, de Wit, Tobias F. Rinke, additional, and Wolday, Dawit, additional

Published
2022
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34. Whole blood versus red cell concentrates for children with severe anaemia: a secondary analysis of the Transfusion and Treatment of African Children (TRACT) trial

Author

George, Elizabeth C, primary, Uyoga, Sophie, additional, M'baya, Bridon, additional, Kyeyune Byabazair, Dorothy, additional, Kiguli, Sarah, additional, Olupot-Olupot, Peter, additional, Opoka, Robert O, additional, Chagaluka, George, additional, Alaroker, Florence, additional, Williams, Thomas N, additional, Bates, Imelda, additional, Mbanya, Dora, additional, Gibb, Diana M, additional, Walker, A Sarah, additional, Maitland, Kathryn, additional, Walker, Sarah A, additional, George, Elizabeth C, additional, Mpoya, Ayub, additional, Frost, Gary, additional, Walsh, Kevin, additional, Nteziyaremye, Julius, additional, Namayanja, Cate, additional, Ssenyondo, Tonny, additional, Passi, George, additional, Muhindo, Rita, additional, Masifa, George, additional, Adong, Ruth, additional, Okalebo, Charles B, additional, Atimango, Emmanuela, additional, Thembo, Nobert, additional, Odong, George, additional, Kiluli, Godfrey, additional, Kapsindet, Job, additional, Kayaga, Julianne, additional, Nabawanuka, Eva, additional, Kadama, Eva, additional, Mukisa, Cynthia Williams, additional, Engoru, Charles, additional, Nakuya, Margaret, additional, Amorut, Denis, additional, Olupot, Moses, additional, Onyas, Pius, additional, Ariimi, Margaret, additional, Itipe, Melda, additional, Atim, Mary G, additional, Abeno, Mary, additional, Okwi, Steven, additional, Kulume, Mary G, additional, Among, Grace, additional, Achipa, Dorreen E, additional, Macharia, Alex, additional, Mellewa, Machpherson, additional, Kennedy, Neil, additional, Kumwenda, Felistas, additional, Sochera, Tiferanji Fatch, additional, Malenga, Albert, additional, Chimalizeni, Yamikani FG, additional, Gushu, Benard, additional, Phiri, Tusekile, additional, Chesale, Amisa, additional, Mitole, Ndaona, additional, Chokani, Ellida, additional, Munthali, Annie, additional, Hensbroek, Michael Boele von, additional, South, Annabelle, additional, Thomason, Margaret J, additional, Baptiste, David, additional, Connon, Roisin, additional, MacCabe, Leanne, additional, Ali, Abdul, additional, Khamis, Kibibi, additional, Madoola, Macreen, additional, Abongo, Grace, additional, Urban, Britta, additional, Heydermann, Robert, additional, Kyomuhendo, Flavia, additional, Nakalanzi, Sarah, additional, Chabuka, John, additional, Mkandawire, Nkhafwire, additional, Fitzgerald, Felicity, additional, Evans, Jennifer A, additional, Molyneux, Elizabeth, additional, Lubega, Irene, additional, Crawley, Jane, additional, Kazembe, Peter, additional, Murphy, Mike, additional, Peto, Tim, additional, Todd, Jim, additional, Mirembe, Grace, additional, Musoka, Philipa, additional, and Tenu, Filemoni, additional

Published
2022
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39. Influence of sex, season and environmental air quality on experimental human pneumococcal carriage acquisition

Author

Cheliotis, Katerina S., primary, Jewell, Christopher P., additional, Solórzano, Carla, additional, Urban, Britta, additional, Collins, Andrea M., additional, Mitsi, Elena, additional, Pojar, Sherin, additional, Nikolaou, Elissavet, additional, German, Esther L., additional, Reiné, Jesús, additional, Gordon, Stephen B., additional, Jochems, Simon P., additional, Rylance, Jamie, additional, and Ferreira, Daniela M., additional

Published
2021
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40. Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection

Author

Guermonprez, Pierre, Helft, Julie, Claser, Carla, Deroubaix, Stephanie, Karanje, Henry, Gazumyan, Anna, Darasse-Jeze, Guillaume, Telerman, Stephanie B., Breton, Gaelle, Schreiber, Heidi A., Frias-Staheli, Natalia, Billerbeck, Eva, Dorner, Marcus, Rice, Charles M., Ploss, Alexander, Klein, Florian, Swiecki, Melissa, Colonna, Marco, Kamphorst, Alice O., Meredith, Matthew, Niec, Rachel, Takacs, Constantin, Mikhail, Fadi, Hari, Aswin, Bosque, David, Eisenreich, Tom, Merad, Miriam, Shi, Yan, Ginhoux, Florent, Renia, Laurent, Urban, Britta C., and Nussenzweig, Michel C.

Subjects
T cells -- Health aspects -- Physiological aspects -- Genetic aspects -- Research, Biological response modifiers -- Health aspects -- Physiological aspects -- Genetic aspects -- Research, Dendritic cells -- Health aspects -- Physiological aspects -- Genetic aspects -- Research, Malaria -- Development and progression -- Genetic aspects -- Research, Biological sciences, Health
Abstract

Innate sensing mechanisms trigger a variety of humoral and cellular events that are essential to adaptive immune responses. Here we describe an innate sensing pathway triggered by Plasmodium infection that [...]

Published
2013
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41. Streptococcus pneumoniae colonisation associates with impaired adaptive immune responses against SARS-CoV-2

Author

Mitsi, Elena, primary, Reiné, Jesús, additional, Urban, Britta C, additional, Solórzano, Carla, additional, Nikolaou, Elissavet, additional, Hyder-Wright, Angela D., additional, Pojar, Sherin, additional, Howard, Ashleigh, additional, Hitchins, Lisa, additional, Glynn, Sharon, additional, Farrar, Madlen, additional, Liatsikos, Konstantinos, additional, Collins, Andrea M, additional, Walker, Naomi F, additional, Hill, Helen, additional, German, Esther L, additional, Cheliotis, Katerina S, additional, Byrne, Rachel L, additional, Williams, Christopher T., additional, Cubas-Atienzar, Ana I, additional, Flecher, Tom, additional, Adams, Emily R, additional, Draper, Simon J, additional, Pulido, David, additional, Beavon, Rohini, additional, Theilacker, Christian, additional, Begier, Elizabeth, additional, Jodar, Luis, additional, Gessner, Bradford D, additional, and Ferreira, Daniela M, additional

Published
2021
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42. Pharmacokinetics and pharmacodynamics of azithromycin in severe malaria bacterial co-infection in African children (TABS-PKPD): a protocol for a Phase II randomised controlled trial

Author

Olupot-Olupot, Peter, primary, Okiror, William, additional, Mnjalla, Hellen, additional, Muhindo, Rita, additional, Uyoga, Sophie, additional, Mpoya, Ayub, additional, Williams, Thomas N, additional, terHeine, Rob, additional, Burger, David M, additional, Urban, Britta, additional, Connon, Roisin, additional, George, Elizabeth C, additional, Gibb, Diana M, additional, Walker, A Sarah, additional, and Maitland, Kathryn, additional

Published
2021
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44. Systemic lupus erythematosus-associated defects in the inhibitory receptor Fc[gamma]RIIb reduce susceptibility to malaria

Author

Clatworthy, Menna R., Willcocks, Lisa, Urban, Britta, Langhorne, Jean, Williams, Tom N., Peshu, Norbert, Watkins, Nicholas A., Floto, R. Andres, and Smith, Kenneth G.C.

Subjects
Systemic lupus erythematosus -- Research, Autoimmunity -- Research, Morbidity -- Causes of, Science and technology
Abstract

Polygenic autoimmune diseases, such as systemic lupus erythematosus (SLE), are a significant cause of morbidity and mortality worldwide. In recent years, functionally important genetic polymorphisms conferring susceptibility to SLE have been identified, but the evolutionary pressures driving their retention in the gene pool remain elusive. A defunctioning, SLE-associated polymorphism of the inhibitory receptor Fc[gamma]RIIb is found at an increased frequency in African and Asian populations, broadly corresponding to areas where malaria is endemic. Here, we show that Fc[gamma]RIIb-deficient mice have increased clearance of malarial parasites (Plasmodium chabaudi chabaudi) and develop less severe disease. In vitro, the human lupus associated Fc[gamma]RIIb polymorphism enhances phagocytosis of Plasmodium falciparum-infected erythrocytes. These results demonstrate that FcyRIIb is important in controlling the immune response to malarial parasites and suggests that the higher frequency of human Fc[gamma]RIIb polymorphisms predisposing to SLE in Asians and Africans may be maintained because these variants reduce susceptibility to malaria. autoimmunity | evolution | Fc[gamma] receptors

Published
2007

45. Longitudinal Profile of Antibody Response to SARS-CoV-2 in Patients with COVID-19 in a Setting from Sub–Saharan Africa: A Prospective Longitudinal Study

Author

Gebrecherkos, Teklay, primary, Kebede, Yazezew, additional, Challa, Feyissa, additional, Abdella, Saro, additional, Gebreegzabher, Atsbeha, additional, Desta, Abraham, additional, Atsbaha, Ataklti Hailu, additional, Tasew, Geremew, additional, Abdulkader, Mahmud, additional, Tesema, Masresha, additional, Tollera, Getachew, additional, Arefaine, Zekarias Gessesse, additional, Schallig, Henk H.D.F., additional, Adams, Emily R., additional, Urban, Britta C., additional, Rinke De Wit, Tobias F., additional, and Wolday, Dawit, additional

Published
2021
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46. Effect of Co-Infection with Intestinal Parasites on COVID-19 Severity: A Prospective Observational Cohort Study

Author

Wolday, Dawit, primary, Gebrecherkos, Teklay, additional, Arefaine, Zekarias Gessesse, additional, Kebede, Yazezew, additional, Gebreegzabher, Atsbeha, additional, Tasew, Geremew, additional, Abdulkader, Mahmud, additional, Abraha, Hiluf Ebuy, additional, Desta, Abraham Aregay, additional, Atsbaha, Ataklti Hailu, additional, Tollera, Getachew, additional, Abdella, Saro, additional, Tesema, Masresha, additional, Abate, Ebba, additional, Endarge, Kidist Lakew, additional, Hundie, Tsegaye Gebreyes, additional, Miteku, Frehiwot Kassahun, additional, Urban, Britta C., additional, Schallig, Henk H.D.F., additional, Harris, Vanessa C., additional, and Rinke De Wit, Tobias F., additional

Published
2021
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49. Copy number, linkage disequilibrium and disease association in the FCGR locus

Author

Niederer, Heather A., Willcocks, Lisa C., Rayner, Tim F., Yang, Wanling, Lau, Yu Lung, Williams, Thomas N., Scott, J. Anthony, Urban, Britta C., Peshu, Norbert, Dunstan, Sarah J., Hien, Tran Tinh, Phu, Nguyen Hoan, Padyukov, Leonid, Gunnarsson, Iva, Svenungsson, Elisabet, Savage, Caroline O., Watts, Richard A., Lyons, Paul A., Clayton, David G., and Smith, Kenneth G.C.

Published
2010
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50. Plasmodium falciparum-infected erythrocytes modulate the maturation of dendritic cells

Author

Urban, Britta C., Ferguson, David J.P., Pain, Arnab, Wllcox, Nick, Plebanski, Magdalena, Austyn, Jonathan M., and Roberts, Dvid J.

Subjects
Malaria -- Research, Antigen presenting cells -- Research, Plasmodium falciparum -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Plasmodium falciparum, the malaria parasite, is a successful human pathogen, and specific immunity to malaria is attributed to the occurrence of cytotoxic lymphocytes or antibodies. It is shown that intact malaria-infected erythrocytes bind to dendritic cells and inhibit their maturation, reducing their ability to stimulate T cells.

Published
1999

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