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1. PdPANA: phagemid display as peptide array for neutralizing antibodies, an engineered in silico vaccine candidate against COVID-19.

Author

Uzcátegui, Javier, Mullah, Khaleel, de Virgini, Daniel Buvat, Mendoza, Andrés, Urdaneta, Rafael, and Naranjo, Alejandra

Subjects
PEPTIDES, COVID-19, RECOMBINANT proteins, PROTEIN engineering, DISPLAY systems, IMMUNOGLOBULINS
Abstract

The COVID-19 pandemic has tested the technical, scientific, and industrial resources of all countries worldwide. Faced with the absence of pharmacological strategies against the disease, an effective plan for vaccinating against SARS-CoV-2 has been essential. Due to the lack of production means and necessary infrastructure, only a few nations could adequately confront this pathogen with a production, storage, and distribution scheme in place. This disease has become endemic in many countries, especially in those that are developing, thus necessitating solutions tailored to their reality. In this paper, we propose an in silico method to guide the design towards a thermally stable, universal, efficient, and safe COVID-19 vaccine candidate against SARS-CoV-2 using bioinformatics, immunoinformatics, and molecular modeling approaches for the selection of antigens with higher immunogenic potential, incorporating them into the surface of the M13 phage. Our work focused on using phagemid display as peptide array for neutralizing antibodies (PdPANA). This alternative approach might be useful during the vaccine development process, since it could bring improvements in terms of costeffectiveness in production, durability, and ease of distribution of the vaccine under less stringent thermal conditions compared to existing methods. Our results suggest that in the heavily glycosylated region of SARS-CoV-2 Spike protein (aa 344–583), from its inter-glycosylated regions, useful antigenic peptides can be obtained to be used in M13 phagemid display system. PdPANA, our proposed method might be useful to overcome the classic shortcoming posed by the phage-display technique (i.e., the time-consuming task of in vitro screening through great sized libraries with non-useful recombinant proteins) and obtain the most ideal recombinant proteins for vaccine design purposes. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Classification by production: an alternative criterion to categorization.

Author

Yáñez, Luis, Rojas, Nidia, Aparicio, Ricardo, Mazzei, Manuel, Maldonado, Eugenio, Fernández, Jairo, Fabbozzo, Nicola, Rincón, Rafael, and Urdaneta, Rafael

Subjects
WATER buffalo milk yield, CROSSBREEDING
Abstract

Copyright of Revista Cientifica de la Facultade de Veterinaria is the property of Universidad del Zulia, Facultad de Ciencias Veterinarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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8. Phlebotomine Sand Flies in Two Endemic Foci of American Visceral Leishmaniasis at Torres Municipality, Lara State, Venezuela

Author

Bonfante-Garrido, Rafael, Urdaneta, Rafael, Urdaneta, Iván, Rodríguez, Ricardo, and Perdomo, Rosa

Subjects
Lara state, Lulzomyia pseudolongipalpis, Revistas, Leishmaniasis visceral americana, Estado Lara, American visceral leishmaniasis, Venezuela, Lutzomyia pseudolongipalpis, Universidad del Zulia (LUZ), Universidad de Los Andes (ULA), Revista Científica
Abstract

Se realizó un estudio entomológico con el fin de conocer las especies flebotominas presentes en dos focos endémicos de leishmaniasis visceral americana (LVA) del estado Lara, Venezuela. Las capturas de flebotominos se realizaron, de enero 1998 a marzo 2000, en dos áreas endémicas localizadas en el municipio Torres: La Rinconada (10° 15 N-69' 57' W) y San Esteban (10° 54' N-69` 55' W). Para ello se colocó la trampa de luz Shannon, entre las 19: 00 y 22: 00 h, y 4 trampas de luz CDC durante toda la noche en el peridomicilio y. además, se realizó la búsqueda activa manual en sus refugios naturales. En La Rinconada se capturaron 7.488 ejemplares, pertenecientes a ocho especies flebotominas, de los cuales 6.782 fueron hembras y 706, machos. Lulzomyia pseudolongipalpis fue la especie predominante (94,59%). En San Esteban fueron capturados 1.032 ejemplares, de los cuales 842 fueron hembras y 190 machos. El 97.96% de los ejemplares fueron identificados como L. pseudolongipalpis. Ninguna de las hembras capturadas en las localidades endémicas estudiadas mostró la presencia de promastigotes de Leishmania spp. en su tracto digestivo. Debido al marcado predominio de L. pseudolongipalpis y la escasa presencia de L. evansi en estas áreas endémicas, L. pseudolongipalpis pudiera tener un rol importante en la transmisión de la leishmaniasis visceral en las localidades estudiadas. An entomological study was undertaken to know the presence of phlebotomine species in two endemic foci of Americam viscera) Leishmaniasis (AVL) at Lara State, Venezuela. The captures of phlebotomine sand flies were carried out, from January 1998 to March 2000, in two endemic areas localized at the Torres municipality: La Rinconada (10° 15 N-69° 57' VV) and San Esteban (10` 54' N-69° 55' VV). For this purpose the Shannon light trap was used, between 19: 00 and 22: 00 h, and 4 CDC light traps left overnight at the peridomicily and, besides, active manual searches in its natural refuges was carried out. At La Rinconada. 7,488 specimens of eight phlebotomine species were caught out of which 6,782 were females and 706, males. Lutzomyia pseudolongipalpis was the predominant species (94.59%). At San Esteban 1,032 specimens were captured of which 842 were females and 190 males. The 97.96% of the specimens were identified as L, pseudolongipalpis. None of the females captured at the endemic localities that were studied showed the presente of Leishmania spp. promastigotes in their digestive tract. Due to the predominance of L. pseudolongipalpis and the low presente of L. evansi at these endemic areas, L, pseudolongipalpis should be regarded as having an important role in the transmission of American visceral leishmaniasis in the localities that were studied. 173 - 177 rbonfant@hotmail.com Bimestral

Published
2009

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