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Your search keyword '"Ushkarev IuA"' showing total 8 results
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8 results on '"Ushkarev IuA"'

1. [Human Na+,K+-ATPase genes. Gene family and pseudogene for beta-subunit]

Author

Ushkarev IuA, Gs, Monastyrskaia, Ne, Broude, Nn, Nikiforova, Da, Bessarab, Orlova MIu, Konstantin Petrukhin, Nn, Modianov, and Ed, Sverdlov

Subjects
Macromolecular Substances, Swine, Multigene Family, Sequence Homology, Nucleic Acid, Molecular Sequence Data, Restriction Mapping, Animals, Humans, Sodium-Potassium-Exchanging ATPase, Kidney, Pseudogenes, HeLa Cells
Published
1990

2. [Study of the receptor for black widow spider neurotoxin. II. Isolation and characteristics of the receptor from bovine brain membranes]

Author

Alexander Petrenko, In, Surkova, Og, Shamotienko, Va, Kovalenko, Vn, Krasnoperov, La, Tret Iakov, Ushkarev IuA, and Ev, Grishin

Subjects
Brain Chemistry, Molecular Weight, Kinetics, Membranes, Receptors, Peptide, Neurotoxins, Animals, Spider Venoms, Cattle, Electrophoresis, Polyacrylamide Gel, Receptors, Cholinergic, Arthropod Venoms, Chromatography, Affinity
Abstract

Biospecific sorbents for isolation of the latrotoxin receptor have been obtained and studied. Binding of the receptor components solubilized from the bovine cerebral cortex membrane to the immobilized toxin critically depends on the presence of calcium ions and the solution ionic strength. The procedure for semipreparative isolation of the highly active receptor preparation with Kd = 9 x 10(-10) M and Bmax = 0.9 nmol/mg has been developed. Binding activity of the isolated receptor is inhibited by heating as well as by proteases and denaturing agents. According to electrophoretic analysis in the presence of SDS the receptor complex contains protein components of molecular mass 200, 160, 79, 43 kDa, the former two being glycoproteins.

3. [Study of the receptor for black widow spider neurotoxin. II. Isolation and characteristics of the receptor from bovine brain membranes].

Author

Petrenko AG, Surkova IN, Shamotienko OG, Kovalenko VA, Krasnoperov VN, Tret'iakov LA, Ushkarev IuA, and Grishin EV

Subjects
Animals, Cattle, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Kinetics, Membranes metabolism, Molecular Weight, Receptors, Cholinergic metabolism, Arthropod Venoms metabolism, Brain Chemistry, Neurotoxins metabolism, Receptors, Cholinergic isolation & purification, Receptors, Peptide, Spider Venoms metabolism
Abstract

Biospecific sorbents for isolation of the latrotoxin receptor have been obtained and studied. Binding of the receptor components solubilized from the bovine cerebral cortex membrane to the immobilized toxin critically depends on the presence of calcium ions and the solution ionic strength. The procedure for semipreparative isolation of the highly active receptor preparation with Kd = 9 x 10(-10) M and Bmax = 0.9 nmol/mg has been developed. Binding activity of the isolated receptor is inhibited by heating as well as by proteases and denaturing agents. According to electrophoretic analysis in the presence of SDS the receptor complex contains protein components of molecular mass 200, 160, 79, 43 kDa, the former two being glycoproteins.

Published
1990

4. [Neurotoxin of the black widow spider and its interaction with receptors from the rat brain].

Author

Ushkarev IuA and Grishin EV

Subjects
Animals, Black Widow Spider, Calcium Chloride pharmacology, Chromatography, Gel, Concanavalin A pharmacology, Electrophoresis, Disc, In Vitro Techniques, Kinetics, Lectins pharmacology, Models, Biological, Neurotoxins isolation & purification, Rats, Receptors, Neurotransmitter drug effects, Spider Venoms isolation & purification, Wheat Germ Agglutinins, Arthropod Venoms metabolism, Brain metabolism, Neurotoxins metabolism, Receptors, Neurotransmitter metabolism, Spider Venoms metabolism
Abstract

A presynaptic neurotoxin isolated from the venom of the Central Asia spider karakurt (Black Widow Spider, Latrodectus mactans tredecimguttatus) is shown to consist of two identical subunits of mol. weight about 118 kDa. The iodinated neurotoxin binds to the rat brain synaptosomal plasma membranes with Kd 0.1 nM (Bmax 0.1 pmol/mg of protein) at 37 degrees C, and with Kd 0.35 nM (Bmax 0.2 pmol/mg of protein) at 5 degrees C. At intermediate temperatures both types of receptors are detectable. It is supposed that the dimeric form of the toxin interacts with a single class of receptors possessing lateral mobility in the membrane. By the use of different bifunctional reagents it is revealed that the neurotoxin interacts with a presynaptic membrane protein of mol. weight 95 kDa. A protein of the same size accompanied by a 71 kDa protein was isolated by the affinity chromatography of solubilized synaptosomal membranes on the absorbent, containing immobilized neurotoxin.

Published
1986

8. [Interaction of black widow spider toxin with bilayer phospholipid membranes].

Author

Sokolov IuV, Ushkarev IuA, Grasso A, Grishin EV, and Lishko VK

Subjects
Animals, Cadmium pharmacology, Dose-Response Relationship, Drug, In Vitro Techniques, Membrane Potentials drug effects, Membranes, Artificial, Permeability, Arthropod Venoms pharmacology, Black Widow Spider, Ion Channels drug effects, Lipid Bilayers metabolism, Phospholipids metabolism, Spider Venoms pharmacology, Spiders
Abstract

Black widow spider toxin (latrotoxin) was studied for its effect on bilayer phospholipid membrane conductance. Under the toxin action membranes became selective with respect to divalent cations. The selectivity decreased in the following order: Ba2+, Sr2+, Ca2+, Mg2+. The ion channels formed by toxin in a planar membrane were effectively blocked by Cd2+. Current-voltage characteristics of the membranes permit concluding that toxin molecules are incorporated into a plane bilayer in the oriented way and the formed channels are potential-dependent.

Published
1983

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