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Your search keyword '"Ustinova, Maija"' showing total 10 results
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10 results on '"Ustinova, Maija"'

7. Development and use of an ESBL coding gene panel in patients undergoing first-line eradication therapy for Helicobacter pylori

Author

Gudra, Dita, primary, Silamikelis, Ivars, additional, Pjalkovskis, Janis, additional, Danenberga, Ilva, additional, Pupola, Darta, additional, Skenders, Girts, additional, Ustinova, Maija, additional, Megnis, Kaspars, additional, Leja, Marcis, additional, Vangravs, Reinis, additional, and Fridmanis, Davids, additional

Published
2022
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8. Oral Microbiome Traits of Type 1 Diabetes and Phenylketonuria Patients in Latvia.

Author

Abola, Iveta, Gudra, Dita, Ustinova, Maija, Fridmanis, Davids, Emulina, Darta Elizabete, Skadins, Ingus, Brinkmane, Anda, Lauga-Tunina, Una, Gailite, Linda, and Auzenbaha, Madara

Subjects
GUT microbiome, TYPE 1 diabetes, AMINO acid metabolism disorders, PEOPLE with diabetes
Abstract

Some metabolic disorder treatments require patients to follow a specific diet or to consume supplements that, over time, can lead to oral microbiome alterations. Well-known disorders requiring such treatment are phenylketonuria (PKU), an inborn error of amino acid metabolism, and type 1 diabetes (T1D), a metabolic disorder that requires a specific diet regimen. Therefore, the aim of this study was to investigate the oral health and microbiome characteristics that might contribute to caries activity and periodontal disease risk in PKU and T1D patients. In this cross-sectional study, 45 PKU patients, 24 T1D patients, and 61 healthy individuals between the ages of 12 and 53 years were examined. Their anamnestic data and dental status were assessed by one dentist. Microbial communities were detected from saliva-isolated DNA using 16S rRNA gene V3–V4 sequencing on Illumina MiSeq sequencing platform. Results revealed that the PKU patient group displayed the highest number of extracted teeth (on average 1.34), carious teeth (on average 4.95), and carious activity (44.44% of individuals) compared to the T1D and CTRL groups. The lowest numbers of filled teeth (on average 5.33) and extracted teeth (on average 0.63) per individual were observed in T1D patients. Gingivitis appeared more often in the T1D group; however, possible risk of periodontal disease was seen in both the T1D and PKU patient groups. The highest number of differentially abundant genera was detected in the PKU group (n = 20), with enrichment of Actinomyces (padj = 4.17 × 10−22), Capnocytophaga (padj = 8.53 × 10−8), and Porphyromonas (padj = 1.18 × 10−5) compared to the CTRL group. In conclusion, the dental and periodontal health of PKU patients was found to be significantly inferior compared to T1D patients and healthy controls. T1D patients showed early signs of periodontal disease. Several genera that correlate with periodontal disease development were found in both groups, thus suggesting that T1D and PKU patients should seek early and regular dental advice and be educated about proper oral hygiene practices. [ABSTRACT FROM AUTHOR]

Published
2023
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9. '15 Latvijas pilsētu notekūdeņu metagenoma taksonomiskais un funkcionālais raksturojums'

Author

Ustinova, Maija, Gudrā, Dita, and Latvijas Universitāte. Bioloģijas fakultāte

Subjects
lielapjoma sekvencēšana, metagenoms, Bioloģija, notekūdeņi, taksonomiskā analīze, funkcionālā analīze
Abstract

Neattīrīti notekūdeņi ir ķīmiski un bioloģiski kompleksa vide, taču tā mikrobioloģiskais sastāvs līdz šim ir maz pētīts. Izmantojot šobrīd pieejamās sekvencēšanas un bioinformātikas tehnoloģijas, ir iespējams raksturot notekūdeņu mikroorganismu kopienas sastāvu dažādos līmeņos. Bakalaura darba mērķis bija taksonomiski un funkcionāli raksturot 15 Latvijas pilsētu notekūdeņu metagenomus. DNS tika izdalīta no neattīrītu, 24 h periodā ievāktu koncentrētu notekūdeņu paraugiem, un tai, izmantojot lielapjoma paralēlo sekvencēšanu, tika veikta visa metagenoma analīze. Datu analīzes rezultātā tika noskaidrots, ka pilsētu notekūdeņu metagenomos ir saskatāmas līdzības sugu, ģints, tipa un gēnu līmeņos, taču būtiskas taksonomiskas un funkcionālas metagenoma kompozīcijas atšķirības tika konstatētas starp pilsētām un atšķirīgiem ražošanas notekūdeņu pieplūdumiem., Untreated wastewater is a chemically and biologically complex environment, but thus far its microbiological composition has been little studied. Currently available sequencing and bioinformatics technologies have enabled characterization of the wastewater microorganism population at different levels. The aim of the study was to taxonomically and functionally characterize the wastewater metagenome of 15 Latvian cities. Untreated 24 hour composite wastewater samples were collected at the wastewater treatment plant. Subsequently, DNA was extracted and whole metagenome sequencing analysis was carried employing massive parallel sequencing technologies. Acquired data showed that there are significant taxonomic and functional differences in the composition of the metagenome between cities and different inflows of industrial water, but some similarities at species, genus, phylum and gene levels were also observed.

Published
2021

10. Dominating taxonomic composition of the early life gut microbiota and influencing factors in infants up to seven months of age in Latvia

Author

Zelča, Egija, Gudrā, Dita, Lūse, Ērika, Peterleviča, Jana, Ustinova, Maija, Fridmanis, Dāvids, Rumba-Rozenfelde, Ingrīda, and Daugule, Ilva

Abstract

It has been hypothesised that the establishment of stable adult microbiota is programmed in infancy, and therefore early life gut colonisation may lead to a lifelong microbiota pattern with significant effects on health. The aim of the study was to analyse the composition of gut microbiota and influencing factors in infants up to seven months of age in Latvia. A cross-sectional study was performed at primary healthcare centres. The parents of healthy infants filled out a questionnaire and brought the child’s faecal sample. 16 rRNS gene sequencing was performed to identify the bacterial taxonomic units. The composition of gut microbiota was compared between children with different risk factors. The final participant sample group included 55 infants with median age 4.0 months. The infant gut microbiota of the sample group had typical and rather healthy microbiota — the main phyla detected were Firmicutesand Actinobacteria, the main family was Bifidobacteriaceaand genus — Bifidobacterium. A significant effect of the type of delivery and feeding type was identified, as well as negative correlation between Lactobacilliand gestational age. Further, it would be important to analyse the changes of microbiota prospectively to identify the association with environmental factors and health status in dynamics.

Published
2022
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