Search

Your search keyword '"Usuelli, Vera"' showing total 172 results
Start Over Author "Usuelli, Vera"
172 results on '"Usuelli, Vera"'

1. Glucagon-like peptide 1 receptor is a T cell-negative costimulatory molecule

Author

Ben Nasr, Moufida, Usuelli, Vera, Dellepiane, Sergio, Seelam, Andy Joe, Fiorentino, Teresa Vanessa, D’Addio, Francesca, Fiorina, Emma, Xu, Cong, Xie, Yanan, Balasubramanian, Hari Baskar, Castillo-Leon, Eduardo, Loreggian, Lara, Maestroni, Anna, Assi, Emma, Loretelli, Cristian, Abdelsalam, Ahmed, El Essawy, Basset, Uccella, Silvia, Pastore, Ida, Lunati, Maria Elena, Sabiu, Gianmarco, Petrazzuolo, Adriana, Ducci, Giacomo, Sacco, Elena, Centofanti, Lucia, Venturini, Massimo, Mazzucchelli, Serena, Mattinzoli, Deborah, Ikehata, Masami, Castellano, Giuseppe, Visner, Gary, Kaifeng, Liu, Lee, Kang Mi, Wang, Zhimin, Corradi, Domenico, La Rosa, Stefano, Danese, Silvio, Yang, Jun, Markmann, James F., Zuccotti, Gian Vincenzo, Abdi, Reza, Folli, Franco, and Fiorina, Paolo

Published
2024
Full Text
View/download PDF

4. eATP and autoimmune diabetes

Author

Loretelli, Cristian, Pastore, Ida, Lunati, Maria Elena, Abdelsalam, Ahmed, Usuelli, Vera, Assi, Emma, Fiorina, Emma, Loreggian, Lara, Balasubramanian, Hari Baskar, Xie, Yanan, Yang, Jun, El Essawy, Basset, Montefusco, Laura, D’Addio, Francesca, Ben Nasr, Moufida, and Fiorina, Paolo

Published
2023
Full Text
View/download PDF

5. Abnormalities of the oculomotor function in type 1 diabetes and diabetic neuropathy

Author

D’Addio, Francesca, Pastore, Ida, Loretelli, Cristian, Valderrama-Vasquez, Alessandro, Usuelli, Vera, Assi, Emma, Mameli, Chiara, Macedoni, Maddalena, Maestroni, Anna, Rossi, Antonio, Lunati, Maria Elena, Morpurgo, Paola Silvia, Gandolfi, Alessandra, Montefusco, Laura, Bolla, Andrea Mario, Ben Nasr, Moufida, Di Maggio, Stefania, Melzi, Lisa, Staurenghi, Giovanni, Secchi, Antonio, Bianchi Marzoli, Stefania, Zuccotti, Gianvincenzo, and Fiorina, Paolo

Published
2022
Full Text
View/download PDF

6. The IGFBP3/TMEM219 pathway regulates beta cell homeostasis

Author

D’Addio, Francesca, Maestroni, Anna, Assi, Emma, Ben Nasr, Moufida, Amabile, Giovanni, Usuelli, Vera, Loretelli, Cristian, Bertuzzi, Federico, Antonioli, Barbara, Cardarelli, Francesco, El Essawy, Basset, Solini, Anna, Gerling, Ivan C., Bianchi, Cristina, Becchi, Gabriella, Mazzucchelli, Serena, Corradi, Domenico, Fadini, Gian Paolo, Foschi, Diego, Markmann, James F., Orsi, Emanuela, Škrha, Jr, Jan, Camboni, Maria Gabriella, Abdi, Reza, James Shapiro, A. M., Folli, Franco, Ludvigsson, Johnny, Del Prato, Stefano, Zuccotti, Gianvincenzo, and Fiorina, Paolo

Published
2022
Full Text
View/download PDF

8. miR-21 antagonism reprograms macrophage metabolism and abrogates chronic allograft vasculopathy

Author

Usuelli, Vera, Ben Nasr, Moufida, D’Addio, Francesca, Liu, Kaifeng, Vergani, Andrea, El Essawy, Basset, Yang, Jun, Assi, Emma, Uehara, Mayuko, Rossi, Chiara, Solini, Anna, Capobianco, Annalisa, Rigamonti, Elena, Potena, Luciano, Venturini, Massimo, Sabatino, Mario, Bottarelli, Lorena, Ammirati, Enrico, Frigerio, Maria, Castillo-Leon, Eduardo, Maestroni, Anna, Azzoni, Cinzia, Loretelli, Cristian, Joe Seelam, Andy, Tai, Albert K., Pastore, Ida, Becchi, Gabriella, Corradi, Domenico, Visner, Gary A., Zuccotti, Gian V., Chau, Nelson B., Abdi, Reza, Pezzolesi, Marcus G., and Fiorina, Paolo

Published
2021
Full Text
View/download PDF

9. The IL-8-CXCR1/2 axis contributes to diabetic kidney disease

Author

Loretelli, Cristian, Rocchio, Francesca, D'Addio, Francesca, Ben Nasr, Moufida, Castillo-Leon, Eduardo, Dellepiane, Sergio, Vergani, Andrea, Abdelsalam, Ahmed, Assi, Emma, Maestroni, Anna, Usuelli, Vera, Bassi, Roberto, Pastore, Ida, Yang, Jun, El Essawy, Basset, Elased, Khalid M., Fadini, Gian Paolo, Ippolito, Elio, Seelam, Andy Joe, Pezzolesi, Marcus, Corradi, Domenico, Zuccotti, Gian Vincenzo, Gallieni, Maurizio, Allegretti, Marcello, Niewczas, Monika Anna, and Fiorina, Paolo

Published
2021
Full Text
View/download PDF

10. Acute and long-term disruption of glycometabolic control after SARS-CoV-2 infection

Author

Montefusco, Laura, Ben Nasr, Moufida, D’Addio, Francesca, Loretelli, Cristian, Rossi, Antonio, Pastore, Ida, Daniele, Giuseppe, Abdelsalam, Ahmed, Maestroni, Anna, Dell’Acqua, Marco, Ippolito, Elio, Assi, Emma, Usuelli, Vera, Seelam, Andy Joe, Fiorina, Roberta Maria, Chebat, Enrica, Morpurgo, Paola, Lunati, Maria Elena, Bolla, Andrea Mario, Finzi, Giovanna, Abdi, Reza, Bonventre, Joseph V., Rusconi, Stefano, Riva, Agostino, Corradi, Domenico, Santus, Pierachille, Nebuloni, Manuela, Folli, Franco, Zuccotti, Gian Vincenzo, Galli, Massimo, and Fiorina, Paolo

Published
2021
Full Text
View/download PDF

13. Embryonic stem cell extracts improve wound healing in diabetic mice

Author

Loretelli, Cristian, Ben Nasr, Moufida, Giatsidis, Giorgio, Bassi, Roberto, Lancerotto, Luca, D’Addio, Francesca, Valderrama-Vasquez, Alessandro, Scherer, Saja Sandra, Salvatore, Luca, Madaghiele, Marta, Abdelsalam, Ahmed, Ippolito, Elio, Assi, Emma, Usuelli, Vera, El Essawy, Basset, Sannino, Alessandro, Pietramaggiori, Giorgio, Zuccotti, Gian Vincenzo, Orgill, Dennis Paul, and Fiorina, Paolo

Published
2020
Full Text
View/download PDF

14. Reversal of experimental autoimmune diabetes with a sCD39/anti-CD3 treatment

Author

Fotino, Carmen, primary, Molano, R. Damaris, additional, Nasr, Moufida Ben, additional, Umland, Oliver, additional, Fraker, Christopher A., additional, Ulissi, Ulisse, additional, Balasubramanian, Hari Baskar, additional, Lunati, Maria Elena, additional, Usuelli, Vera, additional, Seelam, Andy Joe, additional, Khalefa, Salma Ayman, additional, Sala, Christian La, additional, Gimeno, Jennifer, additional, Mendez, Armando J., additional, Ricordi, Camillo, additional, Bayer, Allison L., additional, Fiorina, Paolo, additional, and Pileggi, Antonello, additional

Published
2023
Full Text
View/download PDF

16. TMEM219 regulates the transcription factor expression and proliferation of beta cells.

Author

D'Addio, Francesca, Assi, Emma, Maestroni, Anna, Rossi, Giada, Usuelli, Vera, Petrazzuolo, Adriana, Nardini, Marta, Loretelli, Cristian, Ben Nasr, Moufida, and Fiorina, Paolo

Subjects
PANCREATIC beta cells, ISLANDS of Langerhans, GENE expression, CELL proliferation, TRANSCRIPTION factors, TYPE 1 diabetes, CELL preservation
Abstract

Pancreatic beta cells replenishment is considered the next therapeutic option for type 1 diabetes; while stimulating endogenous beta cells proliferation is the "holy grail" for those patients with exhausted beta cell mass. Here we are demonstrating that the pro-apoptotic receptor TMEM219 is expressed in fetal pancreas, in beta cell precursors and in in vitro embryonic-derived endocrine progenitors. TMEM219 signaling negatively regulates beta cells at early stages and induces Caspase 8-mediated cell death. Pharmacological blockade of TMEM219 further rescued beta cell precursor and proliferation markers, and decreased cell death, both in islets and in in vitro-derived endocrine progenitors, allowing for beta cell preservation. While addressing the upstream controlling TMEM219 expression, we determined the TMEM219 miRNet; indeed, one of those miRNAs, miR-129-2, is highly expressed in human islets, particularly in patients at risk or with established type 1 diabetes. miR-129-2 mimic downregulated TMEM219 expression in islets, in in vitro embryonic-derived endocrine progenitors and in highly proliferating insulinoma-derived cells. Moreover, miR-129-2 inhibitor induced a TMEM219 overexpression in insulinoma-derived cells, which restored cell proliferation and functional markers, thus acting as endogenous regulator of TMEM219 expression. The TMEM219 upstream regulator miR129-2 controls the fate of beta cell precursors and may unleash their regenerative potentials to replenish beta cells in type 1 diabetes. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

17. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes

Author

D'Addio, Francesca, Vergani, Andrea, Potena, Luciano, Maestroni, Anna, Usuelli, Vera, Nasr, Moufida Ben, Bassi, Roberto, Tezza, Sara, Dellepiane, Sergio, Essawy, Basset El, Iascone, Maria, Iacovoni, Attilio, Borgese, Laura, Liu, Kaifeng, Visner, Gary, Dhe-Paganon, Sirano, Corradi, Domenico, Abdi, Reza, Starling, Randall C., Folli, Franco, Zuccotti, Gian Vincenzo, Sayegh, Mohamed H., Heeger, Peter S., Chandraker, Anil, Grigioni, Francesco, and Fiorina, Paolo

Subjects
Heart transplantation -- Usage -- Complications and side effects, Gene mutation -- Research, Cardiac patients -- Health aspects, Transcription factors -- Analysis, T cells -- Research, Health care industry
Abstract

Purinergic receptor-7 (P2X7R) signaling controls Th17 and Th1 generation/differentiation, while NOD-like receptor P3 (NLRP3) acts as a Th2 transcriptional factor. Here, we demonstrated the existence of a P2X7R/NLRP3 pathway in T cells that is dysregulated by a P2X7R intracellular region loss-of-function mutation, leading to NLRP3 displacement and to excessive Th17 generation due to abrogation of the NLRP3-mediated Th2 program. This ultimately resulted in poor outcomes in cardiac-transplanted patients carrying the mutant allele, who showed abnormal Th17 generation. Transient NLRP3 silencing in nonmutant T cells or overexpression in mutant T cells normalized the Th profile. Interestingly, IL-17 blockade reduced Th17 skewing of human T cells in vitro and abrogated the severe allograft vasculopathy and abnormal Th17 generation observed in preclinical models in which P2X7R was genetically deleted. This P2X7R intracellular region mutation thus impaired the modulatory effects of P2X7R on NLRP3 expression and function in T cells and led to NLRP3 dysregulation and Th17 skewing, delineating a high-risk group of cardiac-transplanted patients who may benefit from personalized therapy., Introduction Purinergic receptor-7 (P2X7R), primarily expressed on lymphocytes, senses adenosine 5'-triphosphate (ATP) (1) released extracellularly during cell damage (2, 3) and has been shown to regulate T cell activation (4-6). [...]

Published
2018
Full Text
View/download PDF

18. Nanotargeted Delivery of Immune Therapeutics in Type 1 Diabetes.

Author

Jung, Sungwook, Ben Nasr, Moufida, Bahmani, Baharak, Usuelli, Vera, Zhao, Jing, Sabiu, Gianmarco, Seelam, Andy Joe, Naini, Said Movahedi, Balasubramanian, Hari Baskar, Park, Youngrong, Li, Xiaofei, Khalefa, Salma Ayman, Kasinath, Vivek, Williams, MacKenzie D., Rachid, Ousama, Haik, Yousef, Tsokos, George C., Wasserfall, Clive H., Atkinson, Mark A., and Bromberg, Jonathan S.

Published
2023
Full Text
View/download PDF

19. GLP1R Strives T Cell Negative Costimulation Through a rRNA Gene Repressor Interactome

Author

Ben Nasr, Moufida, primary, Usuelli, Vera, additional, Dellepiane, Sergio, additional, Seelam, Andy Joe, additional, Fiorentino, Teresa Vanessa, additional, D’Addio, Francesca, additional, Fiorina, Emma, additional, Xu, Cong, additional, Xie, Yanan, additional, Balasubramanian, Hari Baskar, additional, Castillo-Leon, Eduardo, additional, Loreggian, Lara, additional, Maestroni, Anna, additional, Assi, Emma, additional, Loretelli, Cristian, additional, Abdelsalem, Ahmed, additional, El Essawy, Basset, additional, Uccella, Silvia, additional, Pastore, Ida, additional, Lunati, Maria Elena, additional, Sabiu, Gianmarco, additional, Petrazzuolo, Adriana, additional, Ducci, Giacomo, additional, Sacco, Elena, additional, Centofanti, Lucia, additional, Venturini, Massimo, additional, Mazzucchelli, Serena, additional, Mattinzoli, Deborah, additional, Ikehata, Masami, additional, Castellano, Giuseppe, additional, Visner, Gary, additional, Kaifeng, Liu, additional, Lee, Kang Mi, additional, Wang, Zhimin, additional, Corradi, Domenico, additional, La Rosa, Stefano, additional, Yang, Jun, additional, Markmann, James F., additional, Zuccotti, Gian Vincenzo, additional, Abdi, Reza, additional, Folli, Franco, additional, and Fiorina, Paolo, additional

Published
2023
Full Text
View/download PDF

20. The IGFBP3/TMEM219 pathway regulates beta cell homeostasis

Author

DAddio, Francesca, Maestroni, Anna, Assi, Emma, Ben Nasr, Moufida, Amabile, Giovanni, Usuelli, Vera, Loretelli, Cristian, Bertuzzi, Federico, Antonioli, Barbara, Cardarelli, Francesco, El Essawy, Basset, Solini, Anna, Gerling, Ivan C., Bianchi, Cristina, Becchi, Gabriella, Mazzucchelli, Serena, Corradi, Domenico, Fadini, Gian Paolo, Foschi, Diego, Markmann, James F., Orsi, Emanuela, Skrha, Jan, Camboni, Maria Gabriella, Abdi, Reza, Shapiro, A. M. James, Folli, Franco, Ludvigsson, Johnny, Del Prato, Stefano, Zuccotti, Gianvincenzo, Fiorina, Paolo, D'Addio, F., Maestroni, A., Assi, E., Ben Nasr, M., Amabile, G., Usuelli, V., Loretelli, C., Bertuzzi, F., Antonioli, B., Cardarelli, F., El Essawy, B., Solini, A., Gerling, I. C., Bianchi, C., Becchi, G., Mazzucchelli, S., Corradi, D., Fadini, G. P., Foschi, D., Markmann, J. F., Orsi, E., Skrha, J., Camboni, M. G., Abdi, R., James Shapiro, A. M., Folli, F., Ludvigsson, J., Del Prato, S., Zuccotti, G., and Fiorina, P.

Subjects
Male, Cell- och molekylärbiologi, Inbred C57BL, cells, cultured, Transgenic, Mice, Mice, Inbred NOD, Insulin-Secreting Cells, middle aged, Homeostasis, animal, membrane protein, Cells, Cultured, Mice, Knockout, Cultured, Reverse Transcriptase Polymerase Chain Reaction, adult, gene expression regulation, Middle Aged, reverse transcriptase polymerase chain reaction, diabetes mellitus, type 1, female, diabetes mellitus, type 2, Female, immunoblotting, signal transduction, Type 2, Type 1, Signal Transduction, Adult, mice, inbred C57BL, Cells, Knockout, Science, mice, knockout, Immunoblotting, Mice, Transgenic, Animals, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Humans, Insulin-Like Growth Factor Binding Protein 3, Membrane Proteins, Mice, Inbred C57BL, Gene Expression Regulation, male, insulin-secreting cell, Diabetes Mellitus, human, mice, inbred NOD, mice, transgenic, homeostasi, Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin), Inbred NOD, insulin-like growth factor binding protein 3, Cell and Molecular Biology
Abstract

In this new study the Authors demonstrated that the IGFBP3/TMEM219 pathway is a physiological regulator of pancreatic beta cell homeostasis and it is dysregulated in diabetes. IGFBP3/TMEM219 targeting may therefore serve as a therapeutic option in diabetes. Loss of pancreatic beta cells is a central feature of type 1 (T1D) and type 2 (T2D) diabetes, but a therapeutic strategy to preserve beta cell mass remains to be established. Here we show that the death receptor TMEM219 is expressed on pancreatic beta cells and that signaling through its ligand insulin-like growth factor binding protein 3 (IGFBP3) leads to beta cell loss and dysfunction. Increased peripheral IGFBP3 was observed in established and at-risk T1D/T2D patients and was confirmed in T1D/T2D preclinical models, suggesting that dysfunctional IGFBP3/TMEM219 signaling is associated with abnormalities in beta cells homeostasis. In vitro and in vivo short-term IGFBP3/TMEM219 inhibition and TMEM219 genetic ablation preserved beta cells and prevented/delayed diabetes onset, while long-term IGFBP3/TMEM219 blockade allowed for beta cell expansion. Interestingly, in several patients cohorts restoration of appropriate IGFBP3 levels was associated with improved beta cell function. The IGFBP3/TMEM219 pathway is thus shown to be a physiological regulator of beta cell homeostasis and is also demonstrated to be disrupted in T1D/T2D. IGFBP3/TMEM219 targeting may therefore serve as a therapeutic option in diabetes. Funding Agencies|SID Lombardia Grant; EFSD/JDRF/Lilly Programme on Type 1 Diabetes Research; Italian Ministry of HealthMinistry of Health, Italy [RF-2016-02362512]; Universita di Milano; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [DK104155]; Juvenile Diabetes Research FoundationJuvenile Diabetes Research Foundation; Enthera S.r.l.

Published
2022

23. The anti-inflammatory and immunological properties of GLP-1 Receptor Agonists

Author

Bendotti, Giulia, primary, Montefusco, Laura, additional, Lunati, Maria Elena, additional, Usuelli, Vera, additional, Pastore, Ida, additional, Lazzaroni, Elisa, additional, Assi, Emma, additional, Seelam, Andy Joe, additional, El Essawy, Basset, additional, Jang, Jun, additional, Loretelli, Cristian, additional, D’Addio, Francesca, additional, Berra, Cesare, additional, Ben Nasr, Moufida, additional, Zuccotti, GianVincenzo, additional, and Fiorina, Paolo, additional

Published
2022
Full Text
View/download PDF

24. Immunogenicity and Safety of SARS-CoV-2 mRNA Vaccines in a Cohort of Patients With Type 1 Diabetes

Author

D’Addio, Francesca, primary, Sabiu, Gianmarco, additional, Usuelli, Vera, additional, Assi, Emma, additional, Abdelsalam, Ahmed, additional, Maestroni, Anna, additional, Seelam, Andy Joe, additional, Ben Nasr, Moufida, additional, Loretelli, Cristian, additional, Mileto, Davide, additional, Rossi, Giada, additional, Pastore, Ida, additional, Montefusco, Laura, additional, Morpurgo, Paola S., additional, Plebani, Laura, additional, Rossi, Antonio, additional, Chebat, Enrica, additional, Bolla, Andrea M., additional, Lunati, Maria Elena, additional, Mameli, Chiara, additional, Macedoni, Maddalena, additional, Antinori, Spinello, additional, Rusconi, Stefano, additional, Gallieni, Maurizio, additional, Berra, Cesare, additional, Folli, Franco, additional, Galli, Massimo, additional, Gismondo, Maria Rita, additional, Zuccotti, Gianvincenzo, additional, and Fiorina, Paolo, additional

Published
2022
Full Text
View/download PDF

26. Indirect and Direct Effects of SARS-CoV-2 on Human Pancreatic Islets

Author

Nasr, Moufida Ben, primary, D’Addio, Francesca, primary, Montefusco, Laura, primary, Usuelli, Vera, primary, Loretelli, Cristian, primary, Rossi, Antonio, primary, Pastore, Ida, primary, Abdelsalam, Ahmed, primary, Maestroni, Anna, primary, Dell’Acqua, Marco, primary, Ippolito, Elio, primary, Assi, Emma, primary, Seelam, Andy Joe, primary, Fiorina, Roberta Maria, primary, Chebat, Enrica, primary, Morpurgo, Paola, primary, Lunati, Maria Elena, primary, Bolla, Andrea Mario, primary, Abdi, Reza, primary, Bonventre, Joseph V., primary, Rusconi, Stefano, primary, Riva, Agostino, primary, Corradi, Domenico, primary, Santus, Pierachille, primary, Clark, Pamela, primary, Nebuloni, Manuela, primary, Baldi, Gabriella, primary, Finzi, Giovanna, primary, Folli, Franco, primary, Zuccotti, Gian Vincenzo, primary, Galli, Massimo, primary, Herold, Kevan C., primary, and Fiorina, Paolo, primary

Published
2022
Full Text
View/download PDF

27. Indirect and Direct Effects of SARS-CoV-2 on Human Pancreatic Islets

Author

Ben Nasr, Moufida, primary, D’Addio, Francesca, additional, Montefusco, Laura, additional, Usuelli, Vera, additional, Loretelli, Cristian, additional, Rossi, Antonio, additional, Pastore, Ida, additional, Abdelsalam, Ahmed, additional, Maestroni, Anna, additional, Dell’Acqua, Marco, additional, Ippolito, Elio, additional, Assi, Emma, additional, Seelam, Andy Joe, additional, Fiorina, Roberta Maria, additional, Chebat, Enrica, additional, Morpurgo, Paola, additional, Lunati, Maria Elena, additional, Bolla, Andrea Mario, additional, Abdi, Reza, additional, Bonventre, Joseph V., additional, Rusconi, Stefano, additional, Riva, Agostino, additional, Corradi, Domenico, additional, Santus, Pierachille, additional, Clark, Pamela, additional, Nebuloni, Manuela, additional, Baldi, Gabriella, additional, Finzi, Giovanna, additional, Folli, Franco, additional, Zuccotti, Gian Vincenzo, additional, Galli, Massimo, additional, Herold, Kevan C., additional, and Fiorina, Paolo, additional

Published
2022
Full Text
View/download PDF

28. Pharmacologically Enhanced Regulatory Hematopoietic Stem Cells Revert Experimental Autoimmune Diabetes and Mitigate Other Autoimmune Disorders

Author

Ben Nasr, Moufida, primary, Robbins, David, additional, Parone, Philippe, additional, Usuelli, Vera, additional, Tacke, Robert, additional, Seelam, Andy-Joe, additional, Driver, Emily, additional, Le, Thuy, additional, Sabouri-Ghomi, Mohsen, additional, Guerrettaz, Lisa, additional, Shoemaker, Daniel, additional, and Fiorina, Paolo, additional

Published
2022
Full Text
View/download PDF

29. PD-1 blockade counteracts post–COVID-19 immune abnormalities and stimulates the anti–SARS-CoV-2 immune response

Author

Loretelli, Cristian, primary, Abdelsalam, Ahmed, additional, D’Addio, Francesca, additional, Ben Nasr, Moufida, additional, Assi, Emma, additional, Usuelli, Vera, additional, Maestroni, Anna, additional, Seelam, Andy Joe, additional, Ippolito, Elio, additional, Di Maggio, Stefania, additional, Loreggian, Lara, additional, Radovanovic, Dejan, additional, Vanetti, Claudia, additional, Yang, Jun, additional, El Essawy, Basset, additional, Rossi, Antonio, additional, Pastore, Ida, additional, Montefusco, Laura, additional, Lunati, Maria Elena, additional, Bolla, Andrea M., additional, Biasin, Mara, additional, Antinori, Spinello, additional, Santus, Pierachille, additional, Riva, Agostino, additional, Zuccotti, Gian Vincenzo, additional, Galli, Massimo, additional, Rusconi, Stefano, additional, and Fiorina, Paolo, additional

Published
2021
Full Text
View/download PDF

32. Abnormalities of the oculomotor function in type 1 diabetes and diabetic neuropathy.

Author

D'Addio, Francesca, Pastore, Ida, Loretelli, Cristian, Valderrama-Vasquez, Alessandro, Usuelli, Vera, Assi, Emma, Mameli, Chiara, Macedoni, Maddalena, Maestroni, Anna, Rossi, Antonio, Lunati, Maria Elena, Morpurgo, Paola Silvia, Gandolfi, Alessandra, Montefusco, Laura, Bolla, Andrea Mario, Ben Nasr, Moufida, Di Maggio, Stefania, Melzi, Lisa, Staurenghi, Giovanni, and Secchi, Antonio

Subjects
TYPE 1 diabetes, DIABETIC neuropathies, ARTIFICIAL pancreases, EYE tracking, EYE movements, PEOPLE with diabetes, HUMAN abnormalities
Abstract

Aims: Abnormalities in the oculomotor system may represent an early sign of diabetic neuropathy and are currently poorly studied. We designed an eye-tracking-based test to evaluate oculomotor function in patients with type 1 diabetes. Methods: We used the SRLab—Tobii TX300 Eye tracker®, an eye-tracking device, coupled with software that we developed to test abnormalities in the oculomotor system. The software consists of a series of eye-tracking tasks divided into 4 classes of parameters (Resistance, Wideness, Pursuit and Velocity) to evaluate both smooth and saccadic movement in different directions. We analyzed the oculomotor system in 34 healthy volunteers and in 34 patients with long-standing type 1 diabetes. Results: Among the 474 parameters analyzed with the eye-tracking-based system, 11% were significantly altered in patients with type 1 diabetes (p < 0.05), with a higher proportion of abnormalities observed in the Wideness (24%) and Resistance (10%) parameters. Patients with type 1 diabetes without diabetic neuropathy showed more frequently anomalous measurements in the Resistance class (p = 0.02). The classes of Velocity and Pursuit were less frequently altered in patients with type 1 diabetes as compared to healthy subjects, with anomalous measurements mainly observed in patients with diabetic neuropathy. Conclusions: Abnormalities in oculomotor system function can be detected in patients with type 1 diabetes using a novel eye-tracking-based test. A larger cohort study may further determine thresholds of normality and validate whether eye-tracking can be used to non-invasively characterize early signs of diabetic neuropathy. Trial: NCT04608890. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

33. Hematopoietic Stem Cells in Type 1 Diabetes

Author

Pastore, Ida, primary, Assi, Emma, additional, Ben Nasr, Moufida, additional, Bolla, Andrea Mario, additional, Maestroni, Anna, additional, Usuelli, Vera, additional, Loretelli, Cristian, additional, Seelam, Andy Joe, additional, Abdelsalam, Ahmed, additional, Zuccotti, Gian Vincenzo, additional, D’Addio, Francesca, additional, and Fiorina, Paolo, additional

Published
2021
Full Text
View/download PDF

35. Immunogenicity and safety of SARS-CoV-2 mRNA vaccines in a cohort of patients with type 1 diabetes.

Author

D'Addio, Francesca, Sabiu, Gianmarco, Usuelli, Vera, Assi, Emma, Abdelsalam, Ahmed, Maestroni, Anna, Seelam, Andy Joe, Ben Nasr, Moufida, Loretelli, Cristian, Mileto, Davide, Rossi, Giada, Pastore, Ida, Montefusco, Laura, Morpurgo, Paola S., Plebani, Laura, Rossi, Antonio, Chebat, Enrica, Bolla, Andrea M., Lunati, Maria Elena, and Mameli, Chiara

Abstract

Patients with type 1 diabetes (T1D) may develop severe outcomes during COVID-19 disease, but their ability to generate an immune response against the SARS-CoV-2 messenger RNA (mRNA) vaccines remains to be established. Here we evaluated the safety, immunogenicity and glycometabolic effects of the SARS-CoV-2 mRNA vaccines in patients with T1D. A total of 375 patients, 326 with T1D and 49 non-diabetics, who received two doses of the SARS-CoV-2 mRNA vaccines (mRNA-1273, BNT162b2) between March and April 2021 at the ASST FBF-Sacco Milan, Italy, were included in this monocentric observational study (NCT04905823). Local and systemic adverse events were reported in both groups after SARS-CoV-2 mRNA vaccination without statistical differences between them. While both T1D patients and non-diabetic subjects exhibited a parallel increase in anti-SARS-CoV-2S titers after vaccination, the vast majority of T1D patients (70% and 78% respectively) did not show any increase in the SARS-CoV-2-specific cytotoxic response as compared to the robust increased observed in all non-diabetic subjects. A reduced secretion of the T cell-related cytokines IL-2 and TNF-alpha in vaccinated patients with T1D was also observed. No glycometabolic alterations were evident in patients with T1D using continuous glucose monitoring during follow-up. Administration of the SARS-CoV-2 mRNA vaccine is associated with an impaired cellular SARS-CoV-2-specific cytotoxic immune response in T1D patients. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

36. Regulatory B Cells in Autoimmune Diabetes

Author

Ben Nasr, Moufida, primary, Usuelli, Vera, additional, Seelam, Andy Joe, additional, D’Addio, Francesca, additional, Abdi, Reza, additional, Markmann, James F., additional, and Fiorina, Paolo, additional

Published
2021
Full Text
View/download PDF

37. Placental proteome abnormalities in women with gestational diabetes and large-for-gestational-age newborns

Author

Assi, Emma, D'Addio, Francesca, Mandò, Chiara, Maestroni, Anna, Loretelli, Cristian, Ben Nasr, Moufida, Usuelli, Vera, Abdelsalam, Ahmed, Seelam, Andy Joe, Pastore, Ida, Magagnotti, Cinzia, Abdi, Reza, El Essawy, Basset, Folli, Franco, Corradi, Domenico, Zuccotti, Gianvincenzo, Cetin, Irene, and Fiorina, Paolo

Subjects
Proteomics, lcsh:RC648-665, endocrine system diseases, diabetes, placenta, Proteome, Cesarean Section, Infant, Newborn, nutritional and metabolic diseases, Genetics/Genomes/Proteomics/Metabolomics, lcsh:Diseases of the endocrine glands. Clinical endocrinology, Fetal Macrosomia, Diabetes, Gestational, Pregnancy, Humans, Female, gestational
Abstract

Introduction Gestational diabetes mellitus (GDM) is the most frequent metabolic complication during pregnancy and is associated with development of short-term and long-term complications for newborns, with large-for-gestational-age (LGA) being particularly common. Interestingly, the mechanism behind altered fetal growth in GDM is only partially understood.Research design and methods A proteomic approach was used to analyze placental samples obtained from healthy pregnant women (n=5), patients with GDM (n=12) and with GDM and LGA (n=5). Effects of altered proteins on fetal development were tested in vitro in human embryonic stem cells (hESCs).Results Here, we demonstrate that the placental proteome is altered in pregnant women affected by GDM with LGA, with at least 37 proteins differentially expressed to a higher degree (p

Published
2020

39. The IGFBP3/TMEM219 pathway regulates beta cell homeostasis.

Author

D'Addio, Francesca, Maestroni, Anna, Assi, Emma, Ben Nasr, Moufida, Amabile, Giovanni, Usuelli, Vera, Loretelli, Cristian, Bertuzzi, Federico, Antonioli, Barbara, Cardarelli, Francesco, El Essawy, Basset, Solini, Anna, Gerling, Ivan C., Bianchi, Cristina, Becchi, Gabriella, Mazzucchelli, Serena, Corradi, Domenico, Fadini, Gian Paolo, Foschi, Diego, and Markmann, James F.

Subjects
PANCREATIC beta cells, INSULIN-like growth factor-binding proteins, HOMEOSTASIS, DEATH receptors
Abstract

Loss of pancreatic beta cells is a central feature of type 1 (T1D) and type 2 (T2D) diabetes, but a therapeutic strategy to preserve beta cell mass remains to be established. Here we show that the death receptor TMEM219 is expressed on pancreatic beta cells and that signaling through its ligand insulin-like growth factor binding protein 3 (IGFBP3) leads to beta cell loss and dysfunction. Increased peripheral IGFBP3 was observed in established and at-risk T1D/T2D patients and was confirmed in T1D/T2D preclinical models, suggesting that dysfunctional IGFBP3/TMEM219 signaling is associated with abnormalities in beta cells homeostasis. In vitro and in vivo short-term IGFBP3/TMEM219 inhibition and TMEM219 genetic ablation preserved beta cells and prevented/delayed diabetes onset, while long-term IGFBP3/TMEM219 blockade allowed for beta cell expansion. Interestingly, in several patients' cohorts restoration of appropriate IGFBP3 levels was associated with improved beta cell function. The IGFBP3/TMEM219 pathway is thus shown to be a physiological regulator of beta cell homeostasis and is also demonstrated to be disrupted in T1D/T2D. IGFBP3/TMEM219 targeting may therefore serve as a therapeutic option in diabetes. In this new study the Authors demonstrated that the IGFBP3/TMEM219 pathway is a physiological regulator of pancreatic beta cell homeostasis and it is dysregulated in diabetes. IGFBP3/TMEM219 targeting may therefore serve as a therapeutic option in diabetes. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

40. Prostaglandin E2 Stimulates the Expansion of Regulatory Hematopoietic Stem and Progenitor Cells in Type 1 Diabetes

Author

Ben Nasr, Moufida, D'Addio, Francesca, Malvandi, Amir Mohammad, Faravelli, Silvia, Castillo-Leon, Eduardo, Usuelli, Vera, Rocchio, Francesca, Letizia, Teresa, El Essawy, Abdel Basset, Assi, Emma, Mameli, Chiara, Giani, Elisa, Macedoni, Maddalena, Maestroni, Anna, Dassano, Alice, Loretelli, Cristian, Paroni, Moira, Cannalire, Giuseppe, Biasucci, Giacomo, Sala, Marco, Biffi, Alessandra, Zuccotti, Gian Vincenzo, and Fiorina, Paolo

Subjects
prostaglandins, PD-L1, CXCR4, Immunology, hematopoietic stem and progenitor cells, Immunology and Allergy, autoimmune diseases, Autoimmune diseases, Hematopoietic stem and progenitor cells, Prostaglandins, Original Research
Abstract

Hematopoietic stem and progenitor cells (HSPCs) are multipotent stem cells that have been harnessed as a curative therapy for patients with hematological malignancies. Notably, the discovery that HSPCs are endowed with immunoregulatory properties suggests that HSPC-based therapeutic approaches may be used to treat autoimmune diseases. Indeed, infusion with HSPCs has shown promising results in the treatment of type 1 diabetes (T1D) and remains the only “experimental therapy” that has achieved a satisfactory rate of remission (nearly 60%) in T1D. Patients with newly diagnosed T1D have been successfully reverted to normoglycemia by administration of autologous HSPCs in association with a non-myeloablative immunosuppressive regimen. However, this approach is hampered by a high incidence of adverse effects linked to immunosuppression. Herein, we report that while the use of autologous HSPCs is capable of improving C-peptide production in patients with T1D, ex vivo modulation of HSPCs with prostaglandins (PGs) increases their immunoregulatory properties by upregulating expression of the immune checkpoint-signaling molecule PD-L1. Surprisingly, CXCR4 was upregulated as well, which could enhance HSPC trafficking toward the inflamed pancreatic zone. When tested in murine and human in vitro autoimmune assays, PG-modulated HSPCs were shown to abrogate the autoreactive T cell response. The use of PG-modulated HSPCs may thus provide an attractive and novel treatment of autoimmune diabetes.

Published
2018

41. 1393-P: Alterations in the Placental Proteome in Gestational Diabetes

Author

ASSI, EMMA, primary, D'ADDIO, FRANCESCA, additional, NASR, MOUFIDA BEN, additional, LORETELLI, CRISTIAN, additional, MAESTRONI, ANNA, additional, USUELLI, VERA, additional, MANDÒ, CHIARA, additional, ROCCHIO, FRANCESCA, additional, CORRADI, DOMENICO, additional, FOLLI, FRANCO, additional, ZUCCOTTI, GIANVINCENZO, additional, CETIN, IRENE, additional, and FIORINA, PAOLO, additional

Published
2019
Full Text
View/download PDF

42. 541-P: The Il-8-CXCR1/2 Axis and Its Targeting in Diabetic Kidney Disease

Author

ROCCHIO, FRANCESCA, primary, NASR, MOUFIDA BEN, additional, D'ADDIO, FRANCESCA, additional, LORETELLI, CRISTIAN, additional, USUELLI, VERA, additional, ASSI, EMMA, additional, NIEWCZAS, MONIKA, additional, ESSAWY, BASSET EL, additional, PEZZOLESI, MARCUS G., additional, CORRADI, DOMENICO, additional, ZUCCOTTI, GIANVINCENZO, additional, and FIORINA, PAOLO, additional

Published
2019
Full Text
View/download PDF

43. Regulatory B Cells in Autoimmune Diabetes.

Author

Nasr, Moufida Ben, Usuelli, Vera, Seelam, Andy Joe, D'Addio, Francesca, Abdi, Reza, Markmann, James F., and Fiorina, Paolo

Subjects
*REGULATORY B cells, *DIABETES, *PHENOTYPES
Abstract

Since they were discovered almost three decades ago, a subset of B cells denoted as regulatory B cells (Bregs) have elicited interest throughout the immunology community. Many investigators have sought to characterize their phenotype and to understand their function and immunosuppressive mechanisms. Indeed, studies in murine models have demonstrated that Bregs possess varied phenotypic markers and could be classified into different subsets whose action and pivotal role depend on the pathological condition or stimuli. Similar conclusions were drawn in clinical settings delineating an analogous Breg population phenotypically resembling the murine Bregs that ultimately may be associated with a state of tolerance. Recent studies suggested that Bregs may play a role in the onset of autoimmune diabetes. This review will focus on deciphering the different subclasses of Bregs, their emerging role in autoimmune diabetes, and their potential use as a cell-based therapeutic. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

44. Islet-Derived eATP Fuels Autoreactive CD8+ T Cells and Facilitates the Onset of Type 1 Diabetes

Author

Tezza, Sara, primary, Ben Nasr, Moufida, additional, D’Addio, Francesca, additional, Vergani, Andrea, additional, Usuelli, Vera, additional, Falzoni, Simonetta, additional, Bassi, Roberto, additional, Dellepiane, Sergio, additional, Fotino, Carmen, additional, Rossi, Chiara, additional, Maestroni, Anna, additional, Solini, Anna, additional, Corradi, Domenico, additional, Giani, Elisa, additional, Mameli, Chiara, additional, Bertuzzi, Federico, additional, Pezzolesi, Marcus G., additional, Wasserfall, Clive H., additional, Atkinson, Mark A., additional, Füchtbauer, Ernst-Martin, additional, Ricordi, Camillo, additional, Folli, Franco, additional, Di Virgilio, Francesco, additional, Pileggi, Antonello, additional, Dhe-Paganon, Sirano, additional, Zuccotti, Gian Vincenzo, additional, and Fiorina, Paolo, additional

Published
2018
Full Text
View/download PDF

45. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes

Author

D’Addio, Francesca, primary, Vergani, Andrea, additional, Potena, Luciano, additional, Maestroni, Anna, additional, Usuelli, Vera, additional, Ben Nasr, Moufida, additional, Bassi, Roberto, additional, Tezza, Sara, additional, Dellepiane, Sergio, additional, El Essawy, Basset, additional, Iascone, Maria, additional, Iacovoni, Attilio, additional, Borgese, Laura, additional, Liu, Kaifeng, additional, Visner, Gary, additional, Dhe-Paganon, Sirano, additional, Corradi, Domenico, additional, Abdi, Reza, additional, Starling, Randall C., additional, Folli, Franco, additional, Zuccotti, Gian Vincenzo, additional, Sayegh, Mohamed H., additional, Heeger, Peter S., additional, Chandraker, Anil, additional, Grigioni, Francesco, additional, and Fiorina, Paolo, additional

Published
2018
Full Text
View/download PDF

47. Embryonic Cell Extracts Ameliorate Wound Healing in Diabetic Mice

Author

LORETELLI, CRISTIAN, primary, NASR, MOUFIDA BEN, additional, HAJ, GHINA AL, additional, ROCCHIO, FRANCESCA, additional, FARAVELLI, SILVIA, additional, MALVANDI, AMIR MOHAMMAD, additional, D'ADDIO, FRANCESCA, additional, MAESTRONI, ANNA, additional, LETIZIA, TERESA, additional, ASSI, EMMA, additional, USUELLI, VERA, additional, LEON, EDUARDO CASTILLO, additional, MERONI, ROBERTA, additional, ZUCCOTTI, GIANVINCENZO, additional, and FIORINA, PAOLO, additional

Published
2018
Full Text
View/download PDF

49. Islet-Derived eATP Fuels Autoreactive CD8Cells and Facilitates the Onset of Type 1 Diabetes

Author

Tezza, Sara, primary, Nasr, Moufida Ben, additional, Vergani, Andrea, additional, DDAddio, Francesca, additional, Usuelli, Vera, additional, Falzoni, Simonetta, additional, Bassi, Roberto, additional, Dellepiane, Sergio, additional, Fotino, Carmen, additional, Rossi, Chiara, additional, Maestroni, Anna, additional, Solini, Anna, additional, Corradi, Domenico, additional, Giani, Elisa, additional, Mameli, Chiara, additional, Bertuzzi, Federico, additional, Pezzolesi, Marcus Guy, additional, Wasserfall, Clive H., additional, Atkinson, Mark A., additional, FFchtbauer, Ernst-Martin, additional, Ricordi, Camillo, additional, Folli, Franco, additional, Di Virgilio, Francesco, additional, Pileggi, Antonello, additional, Dhe-Paganon, Sirano, additional, Zuccotti, Gian Vincenzo, additional, and Fiorina, Paolo, additional

Published
2018
Full Text
View/download PDF

50. Metabolomic Profiling in Individuals with a Failing Kidney Allograft

Author

Bassi, Roberto, primary, Niewczas, Monika A., additional, Biancone, Luigi, additional, Bussolino, Stefania, additional, Merugumala, Sai, additional, Tezza, Sara, additional, D’Addio, Francesca, additional, Ben Nasr, Moufida, additional, Valderrama-Vasquez, Alessandro, additional, Usuelli, Vera, additional, De Zan, Valentina, additional, El Essawy, Basset, additional, Venturini, Massimo, additional, Secchi, Antonio, additional, De Cobelli, Francesco, additional, Lin, Alexander, additional, Chandraker, Anil, additional, and Fiorina, Paolo, additional

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results