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13. Phosphoinositide-specific phospholipase C signalling mediates expression of two phenylalanine ammonia lyase genes induced by salicylic acid in Capsicum chinensecells

Author

Rodas-Junco, Beatriz A., González-Mendoza, Víctor, Muñoz-Sánchez, Armando, Poot-Poot, Wilbert, Vázquez-Flota, Felipe, and Hernández-Sotomayor, S. M. Teresa

Abstract

The role of salicylic acid (SA) in the plant stress response is mediated by phenylalanine ammonia lyase (PAL), a key enzyme in the phenylpropanoid pathway in Capsicum chinensecells. However, the relationship between PALexpression in response to SA and the precise involvement of the phosphoinositide-phospholipase C (PI-PLC) transduction pathway linking these effects remains unclear. A pharmacological approach was used to investigate the role of the PI-PLC pathway in the transcriptional regulation of two different PALgene members in C. chinensesuspension cells exposed to SA. Two putative C. chinense PALgenes (CchPAL1and CchPAL5) were identified after clustering as an independent group in a phylogenetic tree built with 67 Solanaceaegenes, corresponding to six species. The expression of CchPAL1and CchPAL5showed differential patterns in C. chinensecells exposed to salicylic acid. Moreover, PLC inhibitors, such as neomycin and U73122, differentially modified the expression profiles. These findings suggest that the PI-PLC pathway participates in the activation of preferred PAL genes in response to SA in Capsicumcells.

Published
2024
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40. Isolation and functional characterization of two dioxygenasese putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)

Author

Carballo-Uicab, Victor Manuel, primary, Cárdenas-Conejo, Yair, additional, Vallejo-Cardona, Alba Adriana, additional, Aguilar-Espinosa, Margarita, additional, Rodríguez-Campos, Jacobo, additional, Serrano-Posada, Hugo, additional, Narváez-Zapata, José Alberto, additional, Vázquez-Flota, Felipe, additional, and Rivera-Madrid, Renata, additional

Published
2019
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48. Salicylic acid induces vanillin synthesis through the phospholipid signaling pathway in Capsicum chinense cell cultures

Author

Rodas-Junco, Beatriz A, Cab-Guillen, Yahaira, Muñoz-Sanchez, J Armando, Vázquez-Flota, Felipe, Monforte-Gonzalez, Miriam, and Hérnandez-Sotomayor, S M Teresa

Subjects
Phospholipase C, Benzaldehydes, Vanillin, Phospholipase D, lipids (amino acids, peptides, and proteins), Capsicum, Salicylic Acid, Phospholipids, Research Paper, Signal Transduction
Abstract

Signal transduction via phospholipids is mediated by phospholipases such as phospholipase C (PLC) and D (PLD), which catalyze hydrolysis of plasma membrane structural phospholipids. Phospholipid signaling is also involved in plant responses to phytohormones such as salicylic acid (SA). The relationships between phospholipid signaling, SA, and secondary metabolism are not fully understood. Using a Capsicum chinense cell suspension as a model, we evaluated whether phospholipid signaling modulates SA-induced vanillin production through the activation of phenylalanine ammonia lyase (PAL), a key enzyme in the biosynthetic pathway. Salicylic acid was found to elicit PAL activity and consequently vanillin production, which was diminished or reversed upon exposure to the phosphoinositide-phospholipase C (PI-PLC) signaling inhibitors neomycin and U73122. Exposure to the phosphatidic acid inhibitor 1-butanol altered PLD activity and prevented SA-induced vanillin production. Our results suggest that PLC and PLD-generated secondary messengers may be modulating SA-induced vanillin production through the activation of key biosynthetic pathway enzymes.

Published
2014

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