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Mortality and morbidity from tuberculous meningitis (TBM) are common, primarily due to inflammatory response to Mycobacterium tuberculosis infection, yet the underlying mechanisms remain poorly understood. We aimed to uncover genes and pathways associated with TBM pathogenesis and mortality, and determine the best predictors of death, utilizing whole-blood RNA sequencing from 281 Vietnamese adults with TBM, 295 pulmonary tuberculosis (PTB), and 30 healthy controls. Through weighted gene co-expression network analysis, we identified hub genes and pathways linked to TBM severity and mortality, with a consensus analysis revealing distinct patterns between HIV-positive and HIV-negative individuals. We employed multivariate elastic-net Cox regression to select candidate predictors of death, then logistic regression and internal bootstrap validation to choose best predictors. Increased neutrophil activation and decreased T and B cell activation pathways were associated with TBM mortality. Among HIV-positive individuals, mortality associated with increased angiogenesis, while HIV-negative individuals exhibited elevated TNF signaling and impaired extracellular matrix organization. Four hub genes- MCEMP1, NELL2, ZNF354C , and CD4 -were strong TBM mortality predictors. These findings indicate that TBM induces a systemic inflammatory response similar to PTB, highlighting critical genes and pathways related to death, offering insights for potential therapeutic targets alongside a novel four-gene biomarker for predicting outcomes., Competing Interests: HH, LT, TT, DV, AN, JD, NT, DV, NB, DH, NP, HN, LV, MI, GT, NT No competing interests declared, (© 2024, Hai, Thanh Hoang Nhat et al.)

Background: Tuberculous meningitis (TBM) is difficult to diagnose. We investigated whether a 3-gene host response signature in blood can distinguish TBM from other brain infections., Methods: The expression of 3 genes (dual specificity phosphatase 3 [DUSP3], guanylate-binding protein [GBP5], krupple-like factor 2 [KLF2]) was analyzed by RNA sequencing of archived whole blood from 4 cohorts of Vietnamese adults: 281 with TBM, 279 with pulmonary tuberculosis, 50 with other brain infections, and 30 healthy controls. Tuberculosis scores (combined 3-gene expression) were calculated following published methodology and discriminatory performance compared using area under a receiver operator characteristic curve (AUC)., Results: GBP5 was upregulated in TBM compared to other brain infections (P < .001), with no difference in DUSP3 and KLF2 expression. The diagnostic performance of GBP5 alone (AUC, 0.74; 95% confidence interval [CI], .67-.81) was slightly better than the 3-gene tuberculosis score (AUC, 0.66; 95% CI, .58-.73) in TBM. Both GBP5 expression and tuberculosis score were higher in participants with human immunodeficiency virus (HIV; P < .001), with good diagnostic performance of GBP5 alone (AUC, 0.86; 95% CI, .80-.93)., Conclusions: The 3-gene host signature in whole blood has the ability to discriminate TBM from other brain infections, including in individuals with HIV. Validation in large prospective diagnostic study is now required., Competing Interests: Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed., (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)

A novel, rapid, and facile method for one-step sonoelectrochemical synthesis of zinc oxide nanoparticles (UEZ) was introduced in this study. The optimum operating parameters have been selected at a voltage of 7.5 V, KCl concentration of 0.5 M, and the reaction time of 60 min. The as-prepared UEZ were characterized by XRD, SEM, and HRTEM. It was found that the UEZ has a hexagonal wurtzite structure with high crystalline quality, good purity, a size range of 30-100 nm, and good photocatalytic degradation of methylene blue. This work provides a facile route for large-scale synthesizing ZnO nanoparticles via anodization., (© 2024 IOP Publishing Ltd.)

Background: The increasing incidence of multidrug-resistant Gram-negative bacteria causing ventilator-associated pneumonia (VAP) is a global concern. A better understanding of the epidemiology of VAP in Southeast Asia is essential to optimise treatments and patient outcomes., Methods: VAP epidemiology in an intensive care unit in Vietnam was investigated. A prospective cohort study was conducted. Patients who were ventilated for >48 hours, diagnosed with VAP, and had a positive respiratory culture between October 2015 and March 2017 were included. Whole-genome sequencing (WGS) was performed on Acinetobacter baumannii isolates., Results: We identified 125 patients (137 episodes) with VAP from 1,699 admissions. Twelve patients had 2 VAP episodes. The median age was 60 years (interquartile range: 48-70), and 68.8% of patients were male. Diabetes mellitus was the most frequent comorbidity ( N =35, 28%). Acinetobacter baumannii was most frequently isolated in the first VAP episode ( N =84, 67.2%) and was multiply resistant to meropenem, levofloxacin, and amikacin. The 30-day mortality rate was 55.2% ( N =69) and higher in patients infected with A. baumannii ( N =52, 65%). WGS results suggested a complex spread of multiple clones., Conclusions: In an intensive care unit in Vietnam, VAP due to A. baumannii had a high mortality rate, and A. baumannii and K. pneumoniae were multidrug resistant, with carbapenem resistance of 97% and 70%, respectively., (© 2023 The Authors.)

In this work, a new facile one-spot method has been designed to fabricate a magnetic recyclable Fe 3 O 4 /rice husk biochar photocatalyst (FBP) for the removal of Ciprofloxacin (CIP) in aqueous solution. This method combines ultrasonic-assisted impregnation and precipitation, which can overcome the difficulties of long-time reactions, complex procedures, and extreme condition requirements. The successful fabrication of the Fe 3 O 4 /biochar material has been proven by a series of material characterization techniques, including X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Raman, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Photoelectron Spectroscopy (XPS), and vibrating sample magnetometer (VSM). Moreover, the as-product FBP exhibited the excellent ability of photodegrading CIP and the possibility of magnetic recovery from the aqueous solution, suggesting a potential solution for removing antibiotic pollutants in environmental remediation., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

A novel facile combination of precipitation and plasma discharge reaction is successfully employed for one-step synthesis of an α-Fe 2 O 3 -Fe 3 O 4 graphene nanocomposite (GFs). The co-existence and anchoring of hematite (α-Fe 2 O 3 ) and magnetite (Fe 3 O 4 ) nanoparticles onto a graphene sheet in the as synthesized GFs were verified by results of XRD, Raman, SEM, TEM, and XPS. HRTEM characterization was used for confirming the bonding between α-Fe 2 O 3 /Fe 3 O 4 nanoparticles and the graphene sheet. Consequently, GFs shows superior photodegrading performance towards methylene blue (MB), compared to individual α-Fe 2 O 3 /Fe 3 O 4 nanoparticles, as a result of band gap narrowing and the electron-hole pair recombination rate reducing. Moreover, GFs allows a good possibility of separating and recycling under an external-magnetic field, suggesting potential in visible-light-promoted photocatalytic applications., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

A green synthetic approach to synthesize silver nanoparticles (AgNPs) using the stem extract of Piper chaudocanum for highly sensitive colorimetric detection of Hg 2+ with a low limit of detection of 23 nM and easy colorimetric read-out has been reported. In addition, the biosynthesized AgNPs demonstrated efficient antibacterial activity against Escherichia coli , Pseudomonas aeruginosa and Staphylococcus aureus . The morphology and structure of the as-synthesized AgNPs were examined using SEM, TEM, EDX, XRD and FT-IR analyses. The XRD and TEM results confirm the formation of AgNPs with an average particle size of 8-12 nm. The TLC, CC and HPLC revealed that four main compounds, pentacosanoic acid ( 1 ), piperine ( 2 ), β -sitosterol ( 3 ), and campesterol glucoside ( 4 ), isolated from P. chaudocanum extract act as reducing and stabilizing agents for AgNP formation, and piperine plays a vital role in green synthesis. The chemical structures of these compounds were determined by ESI MS, FTIR, and one- and two-dimensional NMR spectroscopic data analysis. This approach is an efficient, green, cost-effective, eco-friendly and promising technique for synthesizing AgNPs with applications in the colorimetric detection of Hg 2+ and antibacterial activity., (© 2023 The Authors.)

Background Healthcare-associated infections (HAIs), including bloodstream infections (BSIs) in the intensive care unit (ICU), are growing global public health problems. While high-income countries have reported the burden of HAIs precisely, low- and middle-income countries (LMICs), including Vietnam, often lack surveillance systems for HAIs. In Vietnam, few reports described HAI-associated BSIs. Therefore, in this study, we aim to clarify the characteristics of HAI-associated BSI in an adult ICU. Materials and methods We conducted a retrospective cohort study of HAI-associated BSI in the adult ICU of Bach Mai Hospital (BMH), Vietnam, between December 2013 and August 2015. For every case identified with bacteremia, we collected characteristics and laboratory findings of the case and followed the length of hospital stay and seven-day and 30-day survival. Predictors of 30-day mortality were analyzed using univariate and multivariate analyses. Results Among the 90 cases identified, the median age of the study cohort was 57 (range: 18-90) years, and 59 (65.6%) were male. Chronic heart disease was the most frequent comorbidity (n = 26, 28.9%). The pathogens isolated were mostly Candida spp. (n = 26, 26.3%) and Enterococcus spp. (n = 19, 19.2%). Among the 90 patients with confirmed HAI-associated BSI, 34 (37.8%) patients survived, while 31 (34.4%) patients died in 30 days. In multivariate analysis, chronic heart disease tended to increase with 30-day all-cause mortality (odds ratio (OR) = 3.5, 95% confidence interval (CI) = 1.0-11.9, p = 0.051). Conclusions Our retrospective cohort study is the largest investigation to describe HAI-associated BSI in an adult ICU in a tertiary care hospital in Vietnam. Improved laboratory detection and infection surveillance systems are needed to reduce HAI-associated BSI., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Inada et al.)

In this study, we provide a simple and green approach to recycle waste zinc carbon batteries for making carbon dots and porous carbon material. The carbon dots are easily synthesized by one green step, the hydrothermal treatment of a carbon rod in a mixture of DI water and pure ethanol to obtain a blue fluorescence under UV light, which can be used directly as a fluorescence ink. The as-prepared carbon dot process give typical dots with a uniform diameter from 3 to 8 nm with a strong slight blue fluorescent. The porous carbon material is also recycled from carbon powder in a waste battery via one green step annealing process without any chemical activation and with a hierarchically porous structure. This porous carbon material is demonstrated as an electrode for symmetrical solid state supercapacitors (SSCs) in a sandwich structure: porous carbon/PVA-KOH/porous carbon. The SSCs using recycled porous carbon electrodes exhibit a good energy density of 4.58 W h kg -1 at a power density of 375 W kg -1 and 97.6% retention after 2000 cycles. The facile one green step of hydrothermal and also that of calcination provide a promising strategy to recycle waste zinc carbon batteries, which transfers the excellent applications., Competing Interests: The authors declare no competing financial interest., (This journal is © The Royal Society of Chemistry.)

Chitinases are enzymes that catalyze the degradation of chitin, a major component of the cell walls of pathogenic fungi and cuticles of insects, gaining increasing attention for the control of fungal pathogens and insect pests. Production of recombinant chitinase in a suitable host can result in a more pure product with less processing time and a significantly larger yield than that produced by native microorganisms. The present study aimed to express the synthetic chi42 gene (syncodChi42), which was optimized from the chi42 gene of Trichoderma asperellum SH16, in Escherichia coli to produce 42 kDa chitinase (Ta-CHI42); then determined the activity of this enzyme, characterizations and in vitro antifungal activity as well as its immunogenicity in mice. The results showed that Ta-CHI42 was overexpressed in E. coli. Analysis of the colloidal chitin hydrolytic activity of purified Ta-CHI42 on an agar plate revealed that this enzyme was in a highly active form. This is a neutral chitinase with pH stability in a range of 6-8 and has an optimum temperature of 45°C with thermal stability in a range of 25-35°C. The chitinolytic activity of Ta-CHI42 was almost completely abolished by 5 mM Zn2+ or 1% SDS, whereas it remained about haft under the effect of 1 M urea, 1% Triton X-100 or 5 mM Cu2+. Except for ions such as Mn2+ and Ca2+ at 5 mM that have enhanced chitinolytic activity; 5 mM of Na+, Fe2+ or Mg2+ ions or 1 mM EDTA negatively impacted the enzyme. Ta-CHI42 at 60 U/mL concentration strongly inhibited the growth of the pathogenic fungus Aspergillus niger. Analysis of western blot indicated that the polyclonal antibody against Ta-CHI42 was greatly produced in mice. It can be used to analyze the expression of the syncodChi42 gene in transgenic plants, through immunoblotting assays, for resistance to pathogenic fungi., (© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

In this study, we report a facile and effective approach for large-scale production of nitrogen-doped TiO 2 nanocrystals (UNTs) by a combination of ultrasonic irradiation and electrochemistry at room temperature using NH 4 NO 3 electrolyte as the nitrogen source. The as-prepared UNTs were then characterized by x-ray diffraction, Raman spectroscopy, x-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, and UV-visible diffuse reflectance spectroscopy. The results indicated that the nitrogen content of UNTs reached 9.3% and bandgap energy of 2.62 eV, thus gave the high photocatalytic degradation of methylene blue under visible light irradiation. The mechanism for the formation of UNTs by ultrasonic-assisted electrochemical approach was also proposed., (© 2021 IOP Publishing Ltd.)

In this study, oxygenated graphene nanosheets (OGNs) were successfully synthesized using a simple electrochemical exfoliation approach and applied to remove methylene blue (MB) in an aqueous solution. The surface morphology and structure of the OGNs were characterized by scanning electron microscopy, transmission electron microscopy, Raman, and x-ray photoelectron spectroscopy. The adsorption performance of OGNs towards aqueous MB was tested by batch experiments. Results showed that a large number of functional groups in OGNs enhanced the removal of MB from the aqueous solution due to the electrostatic interactions between the electrochemically oxygenated groups (e.g. C-OH, C-O, and C=O) and dye molecules. Using Langmuir adsorption isotherm, the maximum MB adsorption capacity (q max ) was determined as high as 476.19 mg g -1 . These results suggested that the as-prepared OGNs is an effective and promising adsorbent for removing MB, which could be studied extensively for color removal in wastewater treatment.