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6. Diagnostic issues faced by a rare disease healthcare network during Covid-19 outbreak: data from the Campania Rare Disease Registry

Author

Limongelli G, Iucolano S, Monda E, Elefante P, De Stasio C, Lubrano I, Caiazza M, Mazzella M, Fimiani F, Galdo M, De Marchi G, Esposito M, Rubino M, Cirillo A, Fusco A, Esposito A, Trama U, Esposito S, Scarano G, Sepe J, Andria G, Orlando V, Menditto E, Chiodini P, Campania Rare Disease, Iolascon A, Franzese A, Sanduzzi Zamparelli A, Tessitore A, Romano A, Venosa A, Nunzia Olivieri A, Bianco A, La Manna A, Cerbone AM, Spasiano A, Agnese Stanziola A, Colao A, De Bellis A, Gambale A, Toriello A, Tufano A, Ciampa A, Maria Risitano A, Pisani A, Russo A, Volpe A, De Martino B, Amato B, De Fusco C, Piscopo C, Selleri C, Tucci C, Pignata C, Cioffi D, Melis D, Pasquali D, De Brasi D, Spitaleri D, Russo D, Martellotta D, De Michele E, Varricchio E, Miraglia Del Giudice E, Coscioni E, Cimino E, Pane F, Tranfa F, Pollio F, Lonardo F, Nuzzi F, Simonelli F, Trojsi F, Habetswallner F, Valentini G, Cerbone G, Parenti G, Tedeschi G, Capasso G, Battista Rossi G, Gaglione G, Sarnelli G, Argenziano G, Bellastella G, De Michele G, Fiorentino G, Spadaro G, Scala I, Santoro L, Zeppa L, Auricchio L, Elio Adinolfi L, Alessio M, Amitrano M, Savanelli MC, Russo MG, Ferrucci MG, Carbone MT, Pellecchia MT, Salerno M, Melone M, Del Donno M, Vitale M, Triggiani M, Della Monica M, Lo Presti M, Tenuta M, Mignogna MD, Schiavulli M, Zacchia M, Brunetti-Pierri N, Iovino P, Moscato P, Iandoli R, Scarpa R, Russo R, Troisi S, Sbordone S, Perrotta S, Fecarotta S, Sampaolo S, Cicalese V., Limongelli, Giuseppe, Iucolano, Stefano, Monda, Emanuele, Elefante, Pasquale, De Stasio, Chiara, Lubrano, Imma, Caiazza, Martina, Mazzella, Marialuisa, Fimiani, Fabio, Galdo, Maria, De Marchi, Giulia, Esposito, Martina, Rubino, Marta, Cirillo, Annapaola, Fusco, Adelaide, Esposito, Augusto, Trama, Ugo, Esposito, Salvatore, Scarano, Gioacchino, Sepe, Joseph, Andria, Generoso, Orlando, Valentina, Menditto, Enrica, Chiodini, Paolo, Iolascon, Achille, Franzese, Adriana, Sanduzzi Zamparelli, Alessandro, Tessitore, Alessandro, Romano, Alfonso, Venosa, Alfredo, Nunzia Olivieri, Alma, Bianco, Andrea, La Manna, Angela, Cerbone, Anna Maria, Spasiano, Anna, Agnese Stanziola, Anna, Colao, Annamaria, De Bellis, Annamaria, Gambale, Antonella, Toriello, Antonella, Tufano, Antonella, Ciampa, Antonio, Maria Risitano, Antonio, Pisani, Antonio, Russo, Antonio, Volpe, Antonio, De Martino, Bernardo, Amato, Bruno, De Fusco, Carmela, Piscopo, Carmelo, Selleri, Carmine, Tucci, Celeste, Pignata, Claudio, Cioffi, Daniela, Melis, Daniela, Pasquali, Daniela, De Brasi, Daniele, Spitaleri, Daniele, De Brasi, Davide, Russo, Domenico, Martellotta, Donata, De Michele, Elisa, Varricchio, Elziario, Miraglia Del Giudice, Emanuele, Coscioni, Enrico, Cimino, Ernesto, Pane, Fabrizio, Tranfa, Fausto, Pollio, Filiberto, Lonardo, Fortunato, Nuzzi, Francesca, Simonelli, Francesca, Trojsi, Francesca, Habetswallner, Francesco, Valentini, Gabriele, Cerbone, Gaetana, Parenti, Giancarlo, Tedeschi, Gioacchino, Capasso, Giovambattista, Battista Rossi, Giovanni, Gaglione, Giovanni, Sarnelli, Giovanni, Argenziano, Giuseppe, Bellastella, Giuseppe, De Michele, Giuseppe, Fiorentino, Giuseppe, Spadaro, Giuseppe, Scala, Iri, Santoro, Lucio, Zeppa, Lucio, Auricchio, Luigi, Elio Adinolfi, Luigi, Alessio, Maria, Amitrano, Maria, Savanelli, Maria Cristina, Russo, Maria Giovanna, Ferrucci, Maria Grazia, Carbone, Maria Teresa, Pellecchia, Maria Teresa, Salerno, Mariacarolina, Melone, Marina, Del Donno, Mario, Vitale, Mario, Triggiani, Massimo, Della Monica, Matteo, Lo Presti, Maurizio, Tenuta, Maurizio, Mignogna, Michele Davide, Schiavulli, Michele, Zacchia, Miriam, Brunetti-Pierri, Nicola, Iovino, Paola, Moscato, Paolo, Iandoli, Raffaele, Scarpa, Raffaele, Russo, Romualdo, Troisi, Salvatore, Sbordone, Sandro, Perrotta, Silverio, Fecarotta, Simona, Sampaolo, Simone, Cicalese, Virgilio, Limongelli, G, Iucolano, S, Monda, E, Elefante, P, De Stasio, C, Lubrano, I, Caiazza, M, Mazzella, M, Fimiani, F, Galdo, M, De Marchi, G, Esposito, M, Rubino, M, Cirillo, A, Fusco, A, Esposito, A, Trama, U, Esposito, S, Scarano, G, Sepe, J, Andria, G, Orlando, V, Menditto, E, Chiodini, P, Campania Rare, Disease, Iolascon, A, Franzese, A, Sanduzzi Zamparelli, A, Tessitore, A, Romano, A, Venosa, A, Nunzia Olivieri, A, Bianco, A, La Manna, A, Cerbone, Am, Spasiano, A, Agnese Stanziola, A, Colao, A, De Bellis, A, Gambale, A, Toriello, A, Tufano, A, Ciampa, A, Maria Risitano, A, Pisani, A, Russo, A, Volpe, A, De Martino, B, Amato, B, De Fusco, C, Piscopo, C, Selleri, C, Tucci, C, Pignata, C, Cioffi, D, Melis, D, Pasquali, D, De Brasi, D, Spitaleri, D, Russo, D, Martellotta, D, De Michele, E, Varricchio, E, Miraglia Del Giudice, E, Coscioni, E, Cimino, E, Pane, F, Tranfa, F, Pollio, F, Lonardo, F, Nuzzi, F, Simonelli, F, Trojsi, F, Habetswallner, F, Valentini, G, Cerbone, G, Parenti, G, Tedeschi, G, Capasso, G, Battista Rossi, G, Gaglione, G, Sarnelli, G, Argenziano, G, Bellastella, G, De Michele, G, Fiorentino, G, Spadaro, G, Scala, I, Santoro, L, Zeppa, L, Auricchio, L, Elio Adinolfi, L, Alessio, M, Amitrano, M, Savanelli, Mc, Russo, Mg, Ferrucci, Mg, Carbone, Mt, Pellecchia, Mt, Salerno, M, Melone, M, Del Donno, M, Vitale, M, Triggiani, M, Della Monica, M, Lo Presti, M, Tenuta, M, Mignogna, Md, Schiavulli, M, Zacchia, M, Brunetti-Pierri, N, Iovino, P, Moscato, P, Iandoli, R, Scarpa, R, Russo, R, Troisi, S, Sbordone, S, Perrotta, S, Fecarotta, S, Sampaolo, S, and Cicalese, V.

Subjects
Pediatrics, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), Home therapy, rare diseases, 030204 cardiovascular system & hematology, Campania region, Covid-19, Italy, patient registry, Disease Outbreaks, 03 medical and health sciences, COVID-19 Testing, 0302 clinical medicine, Pandemic, Health care, medicine, Retrospective analysis, Humans, AcademicSubjects/MED00860, Registries, 030212 general & internal medicine, Medical diagnosis, Pandemics, Retrospective Studies, business.industry, Public Health, Environmental and Occupational Health, Outbreak, General Medicine, Original Article, business, Delivery of Health Care, Rare disease
Abstract

Background The aims of this study were: to investigate the capacity of the rare disease healthcare network in Campania to diagnose patients with rare diseases during the outbreak of Covid-19; and to shed light on problematic diagnoses during this period. Methods To describe the impact of the Covid-19 pandemic on the diagnosis of patients with rare diseases, a retrospective analysis of the Campania Region Rare Disease Registry was performed. A tailored questionnaire was sent to rare disease experts to investigate major issues during the emergency period. Results Prevalence of new diagnoses of rare disease in March and April 2020 was significantly lower than in 2019 (117 versus 317, P Conclusions This study describes the effects of the Covid-19 outbreak on the diagnosis of rare disease in a single Italian region and investigates potential issues of diagnosis and management during this period.

Published
2021
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8. May a comprehensive mineralogical study of a jackstone calculus and some other human bladder stones unveil health and environmental implications?

Author

Mercurio, M., primary, Izzo, F., additional, Gatta, Giacomo Diego, additional, Salzano, L., additional, Lotrecchiano, G., additional, Saldutto, P., additional, Germinario, C., additional, Grifa, C., additional, Varricchio, E., additional, Carafa, A., additional, Di Meo, Maria Chiara, additional, and Langella, A., additional

Published
2021
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11. Different methods to manage dry weight in hemodialysis patients

Author

Puntoni, A., Domenico Giannese, Varricchio, E., D Alessandro, C., Egidi, M. F., and Cupisti, A.

Subjects
lung ultra-sound, hemodialysis, Body Weight, overhydration, Blood Pressure, Organism Hydration Status, Peptide Fragments, Body Water, Renal Dialysis, hemodialysis, lung ultra-sound, bioelectrical impedance analysis, overhydration, dry weight, Natriuretic Peptide, Brain, Body Composition, Electric Impedance, Humans, bioelectrical impedance analysis, dry weight, Lung, Ultrasonography
Abstract

Estimating the euvolemia and dry weight of hemodialysis patients still represents a challenge for the nephrologist, since both dehydration and hyperhydration are associated with intradialytic events and cardiovascular complications in the short and long term. Despite the need for a precise and objective definition of the dry weight for the individual patient on dialysis, this is usually determined on a clinical basis. To obtain greater sensitivity the dosage of natriuretic peptides, Bioelectrical Impedance Analysis (BIA) and, more recently, Lung Ultra-Sound (LUS) can all be used. The BIA allows to estimate the subject's body composition and, in particular, the distribution of body fluids. The presence of hyperhydration, as determined through the BIA, is predictive of an increased mortality in numerous observational studies. In recent years, pulmonary ultrasound has taken on an increasingly important role not only within the cardiology and intensive care units, but also in a nephrology setting, especially in dialysis. The purpose of this article is to analyze the advantages and limitations of the methods that can be used to assess the dry weight of patients undergoing hemodialysis treatment.

Published
2020

14. Histological and immunochemical features of gastrointestinal tract,alveolar macrophages and blood leukocytes of pigs fed with polyphenols.in Annals of Anatomy-Anatomischer Anzeiger10.1016/j.aanat.2016.04.028, special issue Animal Anatomy II, vol 207:126 Varricchio E, Lombardi V, Paolucci M, Viola C, Coccia E, Romania S, Pasquale V, Maruccio L, Arcamone N, Avallone L, Russo F

Author

Varricchio E, Lombardi V, Paolucci M, Viola C, Coccia E, Romania S, Pasquale V, Maruccio L, Arcamone N, Avallone L, Russo F, Varricchio, E, Lombardi, V, Paolucci, M, Viola, C, Coccia, E, Romania, S, Pasquale, V, Maruccio, L, Arcamone, N, Avallone, L, and Russo, F

Subjects
gastrointestinal tract,leukocytes,macrophages,polyphenols in pigs
Abstract

In the last years, great importance has been given to the anti-oxidant and anti-pathogens effects of polyphe- nols. In this report, we describe the effect of polyphenols derived from wastewater olive-oil production on blood leukocytes and alveolar macrophages primary cell cultures and on gastrointestinal tract of pigs. Pigs were fed with the addition of polyphenols to standard diet for three months until their regular slaughter. Superoxide anion assay was performed on leukocytes, extracted and cultured from peripheral blood, and alveolar macrophages, isolated and cultured following Brockmeier method. In addition, histological samples of gastrointestinal tract (from stomach to rectum) were analyzed by ematoxylin-eosin staining (to evaluate the height and length of mu- cosal epithelium and the wideness of leukocytes infiltrate) and immunohistochemical method (using antibody against cyclooxygenase-2/COX-2). The results obtained show low levels of superoxide anion in the samples col- lected from the experimental group with respect to the control, while no significant differences were observed in the gastrointestinal tract, with the exception of leukocytes infiltrate in caecum-colon samples of treated animals. Finally, COX-2 immunopositive cells were found exclusively in the samples collected from control group. We sug- gest that the addition of polyphenols to standard diet reduces the presence of superoxide anion in pig peripheral leukocytes and alveolar macrophages and improves the immune response in the adult pig gut. In conclusion, we propose a possible re-use of this agri-food industry waste, otherwise highly polluting, as feed additives for farm animals

Published
2016

18. Leptin in lizards: a new metabolic factor?

Author

COCCIA E, VARRICCHIO E, PAOLUCCI M., PUTTI, ROSALBA, DONIZZETTI, IMMACOLATA, Leon V. Berhardt, Coccia, E, Varricchio, E, Putti, Rosalba, Donizzetti, Immacolata, and Paolucci, M.

Abstract

Leptin is a recently discovered protein implicated in the regulation of food intake, energy expenditure, and whole-body energy balance in rodents and humans. Leptin is indeed characterized by a vast array of functions. Recently, the increasing amount of global research on leptin has shown an unexpected and copious variety of leptin functions, extending from metabolism to reproduction, hematopoiesis, inflammation, and immunological response. The numerous effects of leptin on tissues and endocrine systems have ultimately led to the coordination of whole-body energy metabolism and promoted the expansion of studies on leptin presence and effects in different animal groups, other than humans and rodents. Although studies carried out so far have almost exclusively utilized lizards as animal models, our knowledge in reptiles is still very limited. Such studies provide good evidence for the presence of leptin in target organs such as fat bodies, liver, pancreas, thyroid, stomach, brain, blood, contributing to the definition of a general picture which recalls that of birds and mammals but is also peculiar of these vertebrates. In this review, we summarized the current literature on leptin in lizards and compared it with the recent findings for birds and mammals

Published
2011

20. Preliminary data on the presence and distribution of immunoreactivity to peptides implicated in the control of food intake in the stomach and small intestine of marine mammals

Author

RUSSO, FINIZIA, ARCAMONE, NADIA, ESPOSITO, VINCENZO, GATTA, CLAUDIA, GARGIULO, GIULIANA, DE GIROLAMO, PAOLO, Giurisato M., Facello B, Varricchio E., Russo, Finizia, Arcamone, Nadia, Esposito, Vincenzo, Giurisato, M., Gatta, Claudia, Facello, B, Gargiulo, Giuliana, DE GIROLAMO, Paolo, and Varricchio, E.

Subjects
food intake, small intestine, peptide, stomach
Published
2009

23. Intermuscular variation post-mortem: association with calpain/calpastatin system

Author

VARRICCHIO E, VILLACCI A, VELOTTO S, CIMINO F, NICASTRO S, DI PRISCO M. R, SCALA F, CRASTO, ANTONIO, Varricchio, E, Villacci, A, Velotto, S, Cimino, F, Nicastro, S, DI PRISCO M., R, Scala, F, and Crasto, Antonio

Subjects
mu calpain expression, calpain and calpastatin system, Western Blot method
Published
2006

29. Innervation of the vas deferens and accessory male genital glands in the water buffalo (Bubalus bubalis). Neurochemical characteristics and relationships to the reproductive activity

Author

MIRABELLA, NICOLA, SQUILLACIOTI, CATERINA, VARRICCHIO E., GENOVESE, ANGELO, PAINO, GIUSEPPE, Mirabella, Nicola, Squillacioti, Caterina, Varricchio, E., Genovese, Angelo, and Paino, Giuseppe

Abstract

Autonomic nerves supplying mammalian male internal genital organs have an important role in the regulation of reproductive function. To ®nd out the relationships between the neurochemical content of these nerves and the reproductive activity, we performed a histochemical and immunohistochem- ical study in a species, the water buffalo, exhibiting a seasonal sexual behaviour. The distribution of noradrenergic and nitric oxide synthase (NOS)- and peptide-containing nerves was evaluated during the mating and non-mating periods. Fresh segments of vas deferens and accessory genital glands were collected immediately after slaughter and immersed in 4% paraformaldehyde. Frozen sections were obtained and processed according to single and double labelling immuno ̄uorescent procedures or NADPH-diaphorase histochemistry. During the mating period, a dense noradrenergic innervation was observed to supply the vas deferens as well as the accessory genital glands. NOS- and peptide- containing nerves were also observed but with a lower density. During the non-mating period noradrenergic nerves dramatically reduced. In addition, neuropeptide Y (NPY)- and vasoactive intestinal peptide (VIP)-containing nerves were also reduced. These ®ndings suggest the presence of complex interactions between androgen hormones and the autonomic nerve supply in the regulation of male water buffalo reproductive functions.

Published
2003

30. Italian acacia honey exhibits lytic effects against the crayfish pathogens Aphanomyces astaci and Fusarium avenaceum.

Author

Sateriale, D., Scioscia, E., Colicchio, R., Pagliuca, C., Salvatore, P., Varricchio, E., Grazia Volpe, M., Paolucci, M., and Pagliarulo, C.

Subjects
ACACIA, APHANOMYCES astaci, ANTIFUNGAL agents, MYCOSES, FUSARIUM, OOMYCETES
Abstract

This study purpose was to evaluate the in vitro inhibitory properties of Italian acacia honey extracts against pathogenic aquatic oomycete/fungal isolates that cause different diseases in crayfish, resulting in an elevated mortality rate. The antimycotic activity of acacia honey aqueous extracts was evaluated against the strain UEF88662 of Aphanomyces astaci (oomycete) and the strain SMM2 of Fusarium avenaceum (fungus). The extracts preparation was carried out with water by a cheap, not complex and organic solvent‐free procedure, with low environmental impact and the higher possibility of large‐scale reproducibility. The anti‐oomycete and antifungal activities were quantitatively evaluated by growth, survival and sporulation microbiological assays. The extracts displayed a dose‐dependent inhibitory efficacy on oomycete and fungal growth and survival, as well as on the production of oomycete and fungal spores. Supported by future in vivo studies, our results encourage the use of natural extracts like honey as innovative tools to counteract mycotic infections. Significance and Impact of the Study: The continuous spread of aquatic fungal disease as the 'crayfish plague' and the 'burn spot disease' has severe ecological and commercial repercussions. Critical factor to prevent further spread is the availability of effective antifungals possibility derived from local natural resources to use in innovative strategies of control and eradication of these diseases. This study provides relevant information about the in vitro anti‐oomycete and antifungal activity of Italian acacia honey aqueous extracts against two highly infectious and dangerous pathogenic species, Aphanomyces astaci and Fusarium avenaceum, that are responsible for important crayfish diseases. Significance and Impact of the Study: The continuous spread of aquatic fungal disease as the 'crayfish plague' and the 'burn spot disease' has severe ecological and commercial repercussions. Critical factor to prevent further spread is the availability of effective antifungals possibility derived from local natural resources to use in innovative strategies of control and eradication of these diseases. This study provides relevant information about the in vitro anti‐oomycete and antifungal activity of Italian acacia honey aqueous extracts against two highly infectious and dangerous pathogenic species, Aphanomyces astaci and Fusarium avenaceum, that are responsible for important crayfish diseases. [ABSTRACT FROM AUTHOR]

Published
2019
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36. Superovulation, Ovum Pick-Up and in vitro embryo production in mediterranean buffaloes bred in Italy

Author

ZICARELLI, LUIGI, CAMPANILE, GIUSEPPE, DI PALO, ROSSELLA, ESPOSITO, LUIGI, BONI R., ROVIELLO S., VARRICCHIO E., LANGELLA L., Zicarelli, Luigi, Boni, R., Campanile, Giuseppe, DI PALO, Rossella, Esposito, Luigi, Roviello, S., Varricchio, E., and Langella, L.

Subjects
Reproduction, follicular development, hormonal treatment
Abstract

The aim of this paper is to review the current situation on superovulation and ovum pick-up in buffalo cows.

Published
1996

47. POST-MORTEM ANALYSIS IN THREE MUSCLES OF RED NECK OSTRICH (STRUTHIO CAMELUS MASSAICUS).

Author

Velotto, S., Varricchio, E., Vitale, C., and Crasto, A.

Subjects
*OSTRICHES, *AUTOPSY, *FAST-twitch muscle fibers, *CALPASTATIN, *CALPAIN, *DISEASES
Abstract

A description of the pelvic limb musculature of ostrich (Red Neck) is given. Muscle tissues were obtained from muscle iliotibialis lateralis pars preacetabularis, muscle femorotibialis medius and muscle fibularis longus. The histochemical results revealed the presence of two types of fibres: slow-twitch fibres (type I) and fast-twitch intermediate fibres (type IIA). A higher incidence of type I compared to type IIA fibres in muscle iliotibialis lateralis pars preacetabularis and muscle femorotibialis medius was noticed. The immunohistochemical results revealed that in this species calpain/calpastatin system was localized in various parts of the muscles, although μ-calpain and m-calpain totally disappeared at 4 days post-mortem. Calpastatin and p-94 were present during all the period considered (0, 2 days, 4 days and 6 days post-mortem). In this study changes in enzymatic activities of μ and m-calpain were observed, particularly the activity of μ and m-calpain decrease rapidly during post mortem storage. [ABSTRACT FROM AUTHOR]

Published
2010

48. Microbiological profile and shelf life of fresh pasta subjected to several cycles of pasteurization

Author

Scioscia, E., Viola, C., Colicchio, R., Pagliuca, C., Volpe, M. G., Michele Di Stasio, Varricchio, E., Salvatore, P., Pagliarulo, C., Scioscia, E., Viola, C., Colicchio, R., Pagliuca, C., Volpe, M. G., Di Stasio, M., Varricchio, E., Salvatore, P., and Pagliarulo, C.

Subjects
Fresh pasta, Shelf-life, Packaging, Pasteurization, Microbiological quality
Abstract

Campania, and mostly Avellino and Benevento, is the main producer of durum wheat and pasta. The fresh pasta has a high rate of humidity, which makes it an appropriate substrate for the growth of microorganisms and it requires protection from microbial contamination and thorough microbiological controls for its commercialization. The shelf-life of a food is the time period in which a product, in certain storage conditions, maintains a level of quality and safety "acceptable". The acceptability of the product is defined by the presence or absence of a certain number of microrganisms identified by microbiological analysis aimed basically to ensure that food meets specific microbiological criteria, established by a rigorous national and community legislation (Reg. EC 852/2004, Reg. EC 2073/2005, Reg. EC 1441/2007). Particularly, it is important to define suitable conditions of pasteurization and packaging in order to preserve the fresh pasta for a long period of commercialization and ensuring the safety of consumers. In this study we evaluated the effects of different cycles of pasteurization on the microbiological quality and safety of the fresh pasta produced by a company of Benevento (Italy) and the effects on shelf-life of the product.Grant: We would like to thank "GAL Alto Tammaro-Terre dei Tratturi" - PSR-2007-2013 - Regione Campania for their financing.

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