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The amount of apically extruded debris following glide-path preparation of mesial root of 120 freshly extracted human mandibular molar teeth using Senseus ProFinder files, PathFile, G-Files, Scout-RaCe files, HyFlex glidepath files and V glide-path two file system is of interest. The Eppendorf tubes were used as test equipment for collecting debris and the average weight of the debris was measured using an electronic micro-balancing system. It was observed that regardless of the file system utilized, debris was expelled from the apex. The G files resulted in a lower quantity of debris being extruded (0.070 ± 0.002 mg). In contrast, the V glide-path two file system exhibited the highest amount of debris extrusion (0.110 ± 0.004 mg) compared to all other file systems., (© 2024 Biomedical Informatics.)