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Your search keyword '"Veloso, Tomás G. R."' showing total 6 results
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6 results on '"Veloso, Tomás G. R."'

3. The plant organs and rhizosphere determine the common bean mycobiome

Author

da Silva, Leandro L., Veloso, Tomás G. R., Manhães, Jonathan H. C., da Silva, Cynthia C., and de Queiroz, Marisa V.

Abstract

The plant microbiota diversity is often underestimated when approaches developed mainly for the identification of cultivable microorganisms are used. High-throughput sequencing allows a deeper understanding of the microbial diversity associated with plants. The amplification of ITS1 was used to analyze fungal diversity in several plant organs and rhizosphere of three common bean (Phaseolus vulgaris) varieties grown in a greenhouse. The fungal diversity diverged between those plant organs and the rhizosphere, with the highest found in the rhizosphere and the lowest in the stem. In each organ different numbers of genus, OTUs were identified, in a total of 283 OTUs evenly distributed among the varieties. In the co-occurrence network, a larger number of positive interactions were found in the organs of the aerial part in all varieties. We observed that the diversity of the endophytic microbiota differed more between plant organs than between common bean varieties. Our results show that the diversity of endophytic fungi can be efficiently accessed with the sequencing of ITS amplicons and that this diversity may vary among distinct plant organs and the rhizosphere of a single plant variety.

Published
2024
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4. Molecular evidence for stimulation of methane oxidation in Amazonian floodplains by ammonia-oxidizing communities

Author

Monteiro, Gabriel G. T. N., primary, Barros, Dayane J., additional, Gabriel, Gabriele V. M., additional, Venturini, Andressa M., additional, Veloso, Tomás G. R., additional, Vazquez, Gisele H., additional, Oliveira, Luciana C., additional, Neu, Vania, additional, Bodelier, Paul L. E., additional, Mansano, Cleber Fernando M., additional, Tsai, Siu M., additional, and Navarrete, Acacio A., additional

Published
2022
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6. Effective microorganisms inoculant: Diversity and effect on the germination of palisade grass seeds.

Author

Santos LFD, Lana RP, Silva MCSD, Veloso TGR, Kasuya MCM, and Ribeiro KG

Subjects
Biodiversity, Crops, Agricultural genetics, Germination drug effects, High-Throughput Nucleotide Sequencing methods, Seeds drug effects, Sequence Analysis, DNA, Sequence Analysis, RNA, Sulfuric Acids pharmacology, Agricultural Inoculants genetics, DNA, Bacterial isolation & purification, DNA, Fungal isolation & purification, Germination physiology, Poaceae growth & development, Seeds growth & development
Abstract

Effective microorganisms (EM) are inoculants formed by fungi and bacteria isolated from soil. EM are commonly used by farmers on agronomic crops to stimulate plant growth, but their composition and their benefits has been controverted. This study aimed to analyze the diversity of microorganisms growing in three EM inoculants, as well as to evaluate their efficiency in the germination of palisade grass seeds. The total DNA of the three EM inoculants was extracted, the 16S rRNA and ITS genes were amplified by PCR and sequenced on the Illumina MiSeq platform. Germination tests were conducted with three type of the EM, in three concentration and two times of the immersion. The bacterial group was the most abundant in EM, followed by fungi. Bacterial operational taxonomic units OTUs were shared by all EMs. Pre-treatments of palisade grass seeds with EMs resulted in a higher germination percentage (% G) and germination speed index (IVG) when EM was used at concentration of 1 or 2% in water. Seed immersion for 5 min was more efficient than immersion for 24 h. We can conclude that EM of different origin can share microbial groups and diversity of microorganisms, besides being an alternative to increase palisade grass seeds germination.

Published
2020
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