15 results on '"Verena Haug"'
Search Results
2. Translating genomic tools to Raman spectroscopy analysis enables high-dimensional tissue characterization on molecular resolution
- Author
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Manuel Sigle, Anne-Katrin Rohlfing, Martin Kenny, Sophia Scheuermann, Na Sun, Ulla Graeßner, Verena Haug, Jessica Sudmann, Christian M. Seitz, David Heinzmann, Katja Schenke-Layland, Patricia B. Maguire, Axel Walch, Julia Marzi, and Meinrad Paul Gawaz
- Subjects
Science - Abstract
Abstract Spatial transcriptomics of histological sections have revolutionized research in life sciences and enabled unprecedented insights into genetic processes involved in tissue reorganization. However, in contrast to genomic analysis, the actual biomolecular composition of the sample has fallen behind, leaving a gap of potentially highly valuable information. Raman microspectroscopy provides untargeted spatiomolecular information at high resolution, capable of filling this gap. In this study we demonstrate spatially resolved Raman “spectromics” to reveal homogeneity, heterogeneity and dynamics of cell matrix on molecular levels by repurposing state-of-the-art bioinformatic analysis tools commonly used for transcriptomic analyses. By exploring sections of murine myocardial infarction and cardiac hypertrophy, we identify myocardial subclusters when spatially approaching the pathology, and define the surrounding metabolic and cellular (immune-) landscape. Our innovative, label-free, non-invasive “spectromics” approach could therefore open perspectives for a profound characterization of histological samples, while additionally allowing the combination with consecutive downstream analyses of the very same specimen.
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- 2023
- Full Text
- View/download PDF
3. Pharmacokinetic and Environmental Risk Assessment of Prime-2-CoV, a Non-Replicating Orf Virus-Based Vaccine against SARS-CoV-2
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Carina Metz, Verena Haug, Melanie Müller, and Ralf Amann
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Orf virus ,poxvirus ,viral vector ,vaccine ,environmental risk assessment ,biodistribution ,Medicine - Abstract
Viral vector vaccines represent a substantial advancement in immunization technology, offering numerous benefits over traditional vaccine modalities. The Orf virus (ORFV) strain D1701-VrV is a particularly promising candidate for vaccine development due to its distinctive attributes, such as a good safety profile, the ability to elicit both humoral and cellular immunity, and its favorable genetic and thermal stability. Despite ORFV’s theoretical safety advantages, such as its narrow host range and limited systemic spread post-inoculation, a critical gap persists between these theoretical benefits and the empirical evidence regarding its in vivo safety profile. This discrepancy underscores the need for comprehensive preclinical validations to bridge this knowledge gap, especially considering ORFV’s use in humans. Our research introduces Prime-2-CoV, an innovative ORFV-based vaccine candidate against COVID-19, designed to elicit a robust immune response by expressing SARS-CoV-2 Nucleocapsid and Spike proteins. Currently under clinical trials, Prime-2-CoV marks the inaugural application of ORFV in human subjects. Addressing the aforementioned safety concerns, our extensive preclinical evaluation, including an environmental risk assessment (ERA) and detailed pharmacokinetic studies in rats and immunocompromised NOG mice, demonstrates Prime-2-CoV’s favorable pharmacokinetic profile, negligible environmental impact, and minimal ERA risks. These findings not only affirm the vaccine’s safety and efficacy but also pioneer the use of ORFV-based therapeutics, highlighting its potential for wider therapeutic applications.
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- 2024
- Full Text
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4. Intermittent Dyspnea and Cyanosis in a Newborn Caused by a Hairy Polyp
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Sarah Lignitz, Verena Haug, Britta Siegmund, Wolf Jürgen Mann, Wiltrud Coerdt, and Eva Mildenberger
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Pediatrics ,RJ1-570 - Published
- 2014
- Full Text
- View/download PDF
5. Datasets used for the publication 'XXX'
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Manuel Sigle, Anne-Katrin Rohlfing, Sophia Scheuermann, Na Sun, Ulla Graeßner, Verena Haug, Jessica Sudmann, Christian Seitz, David Heinzmann, Katja Schenke-Layland, Axel Walch, Julia Marzi, and Meinrad Paul Gawaz
- Abstract
Raman spectroscopy data from a section of subendocardial fibrosis, which was mainly used to establish the "Spectromics" approach with established bioinformatic tools. Samples: S01 (subpapillary fibrosis): Raman data, Raman Intensity image at 2940cm-1, H&E staining  
- Published
- 2023
- Full Text
- View/download PDF
6. Geschichte und Moral. Paradoxien pädagogischer Geschichtsvermittlung
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Verena Haug
- Published
- 2021
7. Verunsichernde Orte: Selbstverständnis und Weiterbildung in der Gedenkstättenpädagogik
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Monique Eckmann, Christian Geißler, Uta George, Verena Haug, Wolf Kaiser, Imke Scheurich, Helmut Wretzel, Oliver von Wrochem and Monique Eckmann, Christian Geißler, Uta George, Verena Haug, Wolf Kaiser, Imke Scheurich, Helmut Wretzel, Oliver von Wrochem
- Published
- 2013
8. Literaturbesprechung: Johanna Gehmacher, Klara Löffler (Hg.): Storylines and Blackboxes. Autobiographie und Zeugenschaft in der Nachgeschichte von Nationalsozialismus und Zweitem Weltkrieg, Wien: new academic press 2017 (Beiträge des VWI zur Holocaustforschung, Band 4, hg. vom Wiener Wiesenthal Institut für Holocaust- Studien), 260 Seiten, 22,00 €
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Verena Haug
- Subjects
General Engineering - Abstract
Bibliographie: Haug, Verena: Johanna Gehmacher, Klara Loffler (Hg.): Storylines and Blackboxes. Autobiographie und Zeugenschaft in der Nachgeschichte von Nationalsozialismus und Zweitem Weltkrieg, Wien: new academic press 2017 (Beitrage des VWI zur Holocaustforschung, Band 4, hg. vom Wiener Wiesenthal Institut fur Holocaust-Studien), 260 Seiten, 22,00 €., BIOS – Zeitschrift fur Biographieforschung, Oral History und Lebensverlaufsanalysen, 1-2/2017, S. 304-307. https://doi.org/10.3224/bios.v30i1-2.19
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- 2019
9. Antidepressants Rescue Stress-Induced Disruption of Synaptic Plasticity via Serotonin Transporter–Independent Inhibition of L-Type Calcium Channels
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Alexandra Dorner, Max Horn, Kristin Clark, Sibylle Frase, Norbert Klugbauer, Tanja Vo Van, Claus Normann, Josef Bischofberger, Knut Biber, Jonas Scholliers, Patrick Münzer, Christoph Nissen, Verena Haug, Gabriel Seifert, Tsvetan Serchov, and Gregor von Wolff
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Male ,0301 basic medicine ,Patch-Clamp Techniques ,Pyridines ,Nonsynaptic plasticity ,Hippocampus ,Synaptic Transmission ,Piperazines ,Membrane Potentials ,0302 clinical medicine ,Cadmium Chloride ,Homeostatic plasticity ,Serotonin transporter ,Synaptic scaling ,biology ,Age Factors ,RNA-Binding Proteins ,Calcium Channel Blockers ,Antidepressive Agents ,Paroxetine ,Hindlimb Suspension ,Female ,Serotonin Antagonists ,Rats, Transgenic ,Psychology ,Selective Serotonin Reuptake Inhibitors ,Serotonin ,Calcium Channels, L-Type ,Nifedipine ,CHO Cells ,In Vitro Techniques ,Transfection ,03 medical and health sciences ,Cricetulus ,Animal models of depression ,Metaplasticity ,Animals ,Humans ,Rats, Wistar ,Swimming ,Biological Psychiatry ,Electric Stimulation ,Rats ,Disease Models, Animal ,HEK293 Cells ,030104 developmental biology ,Synaptic fatigue ,Fluvoxamine ,Synaptic plasticity ,biology.protein ,Neuroscience ,Stress, Psychological ,030217 neurology & neurosurgery - Abstract
Background Long-term synaptic plasticity is a basic ability of the brain to dynamically adapt to external stimuli and regulate synaptic strength and ultimately network function. It is dysregulated by behavioral stress in animal models of depression and in humans with major depressive disorder. Antidepressants have been shown to restore disrupted synaptic plasticity in both animal models and humans; however, the underlying mechanism is unclear. Methods We examined modulation of synaptic plasticity by selective serotonin reuptake inhibitors (SSRIs) in hippocampal brain slices from wild-type rats and serotonin transporter (SERT) knockout mice. Recombinant voltage-gated calcium (Ca2+) channels in heterologous expression systems were used to determine the modulation of Ca2+ channels by SSRIs. We tested the behavioral effects of SSRIs in the chronic behavioral despair model of depression both in the presence and in the absence of SERT. Results SSRIs selectively inhibited hippocampal long-term depression. The inhibition of long-term depression by SSRIs was mediated by a direct block of voltage-activated L-type Ca2+ channels and was independent of SERT. Furthermore, SSRIs protected both wild-type and SERT knockout mice from behavioral despair induced by chronic stress. Finally, long-term depression was facilitated in animals subjected to the behavioral despair model, which was prevented by SSRI treatment. Conclusions These results showed that antidepressants protected synaptic plasticity and neuronal circuitry from the effects of stress via a modulation of Ca2+ channels and synaptic plasticity independent of SERT. Thus, L-type Ca2+ channels might constitute an important signaling hub for stress response and for pathophysiology and treatment of depression.
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- 2018
10. Comparison of three clinical rating scales in Friedreich ataxia (FRDA)
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Suzette Heck, Stefanie Wolf, Ludger Schöls, Jan-Markus Dörr, Jörg B. Schulz, Verena Haug, Katrin Bürk, Anja Ivo, Sascha Hering, Thomas Klockgether, Konstantinos Dimitriadis, Dagmar Timmann, Susanne Ratzka, Tobias Lindig, T. Schmitz-Hübsch, Ingrid Degen, Ulrike Mälzig, Bernd Kruse, S Boesch, and Thomas Klopstock
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0303 health sciences ,medicine.medical_specialty ,Ataxia ,Psychometrics ,Validity ,3. Good health ,Developmental psychology ,03 medical and health sciences ,Inter-rater reliability ,0302 clinical medicine ,Physical medicine and rehabilitation ,Neurology ,Rating scale ,Severity of illness ,medicine ,Scale size ,International Cooperative Ataxia Rating Scale ,Neurology (clinical) ,medicine.symptom ,Psychology ,030217 neurology & neurosurgery ,030304 developmental biology - Abstract
To test the validity and reliability of the scale for the assessment and rating of ataxia (SARA) in Friedreich ataxia (FRDA). SARA is limited to eight items and can be performed rapidly. Ninety-six patients with a molecular genetic diagnosis of FRDA were rated using three different clinical scales, the FRDA Rating Scale (FARS), the International Cooperative Ataxia Rating Scale (ICARS), and SARA. Despite considerable discrepancies in scale size and subscale structure, SARA total scores were significantly correlated with ICARS (r = 0.953, P < 0.0001) and FARS (r = 0.938, P < 0.0001) total scores. SARA total scores also correlated with the activities of daily living (ADL, r = 0.929, P < 0.0001). Although originally developed for the use in dominantly inherited ataxias, which are primarily ataxias of the cerebellar type, SARA can also be used successfully to assess afferent ataxia, which is the predominant form in FRDA. Because SARA is characterized by high interrater reliability and practicability, SARA is applicable and well suited forclinical trials of FRDA.
- Published
- 2009
11. Unilateral Venous Thalamic Infarction in a Child Mimicking a Thalamic Tumor
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Barbara Zieger, Volker Mall, Rudolf Korinthenberg, Verena Haug, M. Linder-Lucht, and Irina Mader
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Brain Infarction ,medicine.medical_specialty ,Functional Laterality ,Magnetic resonance angiography ,Thalamus ,Heparin-induced thrombocytopenia ,medicine ,Humans ,Vein ,Venous Thrombosis ,medicine.diagnostic_test ,Brain Neoplasms ,business.industry ,Infant ,Magnetic resonance imaging ,medicine.disease ,Magnetic Resonance Imaging ,Thrombosis ,Venous thrombosis ,medicine.anatomical_structure ,Hemiparesis ,Pediatrics, Perinatology and Child Health ,Female ,Neurology (clinical) ,Radiology ,Intracranial Thrombosis ,medicine.symptom ,Tomography, X-Ray Computed ,business ,Magnetic Resonance Angiography ,Straight sinus - Abstract
Unilateral thalamic infarction is a rare condition in adults. This is a case report of a young child presenting with left-sided hemiparesis of sudden onset due to an unilateral venous thalamic infarction. This was attributed to an asymmetric thrombosis of the cerebral internal veins, a partial thrombosis of the vein of Galen and straight sinus. Magnetic resonance imaging resulted primarily in the differential diagnosis of a cerebral tumor or an intracerebral abscess, leading to stereotactic puncture. Subsequent magnetic resonance venography facilitated the correct diagnosis. Heparin-induced thrombocytopenia necessitated anticoagulation treatment with hirudin and later, warfarin. The patient made a complete recovery. We conclude that unclear unilateral thalamic lesions might be symptomatic of a cerebral deep venous thrombosis and might mimic a thalamic tumor. In uncertain cases, we suggest rapid performance of magnetic resonance angiography.
- Published
- 2009
12. High creatine kinase levels and white matter changes: clinical and genetic spectrum of congenital muscular dystrophies with laminin alpha-2 deficiency
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Maria de los Angeles Beytía, Constantin Anastasopoulos, Verena Haug, Gabriele Dekomien, Sabine Hoffjan, and Janbernd Kirschner
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,medicine.disease_cause ,Polymorphism, Single Nucleotide ,Muscular Dystrophies ,Young Adult ,Laminin ,medicine ,Humans ,High creatine kinase ,Child ,Muscle, Skeletal ,Molecular Biology ,Gene ,Creatine Kinase ,Genetic Association Studies ,Mutation ,Muscle biopsy ,medicine.diagnostic_test ,biology ,Muscle weakness ,Genetic Variation ,Infant ,Cell Biology ,medicine.disease ,Laminin, alpha 2 ,White Matter ,biology.protein ,Congenital muscular dystrophy ,Female ,medicine.symptom - Abstract
Primary deficiency of laminin alpha-2 due to mutations in the LAMA2 gene accounts for 30% of all patients with congenital muscular dystrophy. Here, we present seven patients with partial or total laminin alpha-2 deficiency (MDC1A) with a wide clinical spectrum, ranging from ambulant patients to patients who were never able to stand or sit. We identified two pathogenic mutations in the LAMA2 gene in all patients except for one patient in whom only one mutation was found. Six of the mutations were previously undescribed. In some of the milder cases, laminin alpha-2 expression in the muscle biopsy was only slightly reduced. These findings emphasize that analysis of the LAMA2 gene might be necessary in patients with muscle weakness, cerebral white matter changes and high creatine kinase levels, even in the presence of laminin alpha-2 in the muscle biopsy.
- Published
- 2013
13. Whole-body vibration training in children with Duchenne muscular dystrophy and spinal muscular atrophy
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Verena Haug, Janbernd Kirschner, Eckhard Schönau, Isabel J. Schubert, J. Vry, and Oliver Semler
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Male ,medicine.medical_specialty ,Adolescent ,Duchenne muscular dystrophy ,Pilot Projects ,Vibration ,Muscular Atrophy, Spinal ,medicine ,Whole body vibration ,Humans ,Muscle Strength ,Range of Motion, Articular ,Child ,Muscle contracture ,biology ,business.industry ,General Medicine ,Spinal muscular atrophy ,Recovery of Function ,medicine.disease ,SMA ,Exercise Therapy ,Muscular Dystrophy, Duchenne ,Supportive psychotherapy ,Pediatrics, Perinatology and Child Health ,Ambulatory ,biology.protein ,Physical therapy ,Creatine kinase ,Female ,Neurology (clinical) ,business - Abstract
Introduction Whole-body-vibration training is used to improve muscle strength and function and might therefore constitute a potential supportive therapy for neuromuscular diseases. Objective To evaluate safety of whole-body vibration training in ambulatory children with Duchenne muscular dystrophy (DMD) and spinal muscular atrophy (SMA). Methods 14 children with DMD and 8 with SMA underwent an 8-week vibration training programme on a Galileo MedM ® at home (3 × 3 min twice a day, 5 days a week). Primary outcome was safety of the training, assessed clinically and by measuring serum creatine kinase levels. Secondary outcome was efficacy as measured by changes in time function tests, muscle strength and angular degree of dorsiflexion of the ankles. Results All children showed good clinical tolerance. In boys with DMD, creatine kinase increased by 56% after the first day of training and returned to baseline after 8 weeks of continuous whole-body vibration training. No changes in laboratory parameters were observed in children with SMA. Secondary outcomes showed mild, but not significant, improvements with the exception of the distance walked in the 6-min walking test in children with SMA, which rose from 371.3 m to 402.8 m ( p Interpretation Whole-body vibration training is clinically well tolerated in children with DMD and SMA. The relevance of the temporary increase in creatine kinase in DMD during the first days of training is unclear, but it is not related to clinical symptoms or deterioration.
- Published
- 2013
14. Late-onset autosomal dominant limb girdle muscular dystrophy and Paget's disease of bone unlinked to the VCP gene locus
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Michael Kottlors, Markus Heitzer, Janbernd Kirschner, Antje Bornemann, Ralf Schwarzwald, Verena Haug, Klaus Mueller, Angela Huebner, Olaf Moske-Eick, Wolfram Kress, and Sabine Krause
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Adult ,Male ,medicine.medical_specialty ,Valosin-containing protein ,Cell Cycle Proteins ,Myotonic dystrophy ,Valosin Containing Protein ,Internal medicine ,Germany ,medicine ,Facioscapulohumeral muscular dystrophy ,Humans ,Family ,Muscular dystrophy ,Age of Onset ,Myopathy ,Muscle, Skeletal ,Adenosine Triphosphatases ,Leg ,Muscle biopsy ,biology ,medicine.diagnostic_test ,business.industry ,Middle Aged ,medicine.disease ,Osteitis Deformans ,Pedigree ,Paget's disease of bone ,Endocrinology ,Phenotype ,Neurology ,Muscular Dystrophies, Limb-Girdle ,Mutation ,biology.protein ,Female ,Neurology (clinical) ,medicine.symptom ,business ,Limb-girdle muscular dystrophy - Abstract
The broadwide spectrum of differential diagnoses of autosomal dominant muscular dystrophies in adults can be specified by additional features. The combination of late-onset muscular dystrophy, rimmed vacuoles and inclusion bodies in the muscle biopsy, and Paget's disease of bone suggests a mutation in the Valosin-containing protein gene (VCP, p97 or CDC48) even without dementia. We report on a German family with late-onset autosomal dominant muscular dystrophy starting in the pelvic girdle about age 40years, a subsequent rapidly-progressing course, high alkaline phosphatase and Paget's disease of bone. Clinical examination revealed no cognitive impairment. Histology showed myopathic changes with rimmed vacuoles and inclusion bodies on muscle biopsy. Mutations in VCP, filamin C, desmin, alphaB-crystallin, ZASP and myosin heavy chains 2 and 7 as well as the genes for facioscapulohumeral muscular dystrophy, Myotonic Dystrophy I and II, and LGMD1A-G were excluded by a combination of linkage analysis and direct sequencing. The family presented here suggests that a yet-unknown genetic defect can give rise to an autosomal dominant myopathy with Paget's disease but without dementia.
- Published
- 2009
15. Comparison of three clinical rating scales in Friedreich ataxia (FRDA)
- Author
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Katrin, Bürk, Ulrike, Mälzig, Stefanie, Wolf, Suzette, Heck, Konstantinos, Dimitriadis, Tanja, Schmitz-Hübsch, Sascha, Hering, Tobias M, Lindig, Verena, Haug, Dagmar, Timmann, Ingrid, Degen, Bernd, Kruse, Jan-Markus, Dörr, Susanne, Ratzka, Anja, Ivo, Ludger, Schöls, Sylvia, Boesch, Thomas, Klockgether, Thomas, Klopstock, and Jörg B, Schulz
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Adult ,Male ,Principal Component Analysis ,Adolescent ,Psychometrics ,Statistics as Topic ,Reproducibility of Results ,Middle Aged ,Severity of Illness Index ,Disability Evaluation ,Young Adult ,Friedreich Ataxia ,Outcome Assessment, Health Care ,Humans ,Female ,Child ,Aged - Abstract
To test the validity and reliability of the scale for the assessment and rating of ataxia (SARA) in Friedreich ataxia (FRDA). SARA is limited to eight items and can be performed rapidly. Ninety-six patients with a molecular genetic diagnosis of FRDA were rated using three different clinical scales, the FRDA Rating Scale (FARS), the International Cooperative Ataxia Rating Scale (ICARS), and SARA. Despite considerable discrepancies in scale size and subscale structure, SARA total scores were significantly correlated with ICARS (r = 0.953, P0.0001) and FARS (r = 0.938, P0.0001) total scores. SARA total scores also correlated with the activities of daily living (ADL, r = 0.929, P0.0001). Although originally developed for the use in dominantly inherited ataxias, which are primarily ataxias of the cerebellar type, SARA can also be used successfully to assess afferent ataxia, which is the predominant form in FRDA. Because SARA is characterized by high interrater reliability and practicability, SARA is applicable and well suited forclinical trials of FRDA.
- Published
- 2009
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