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7. ANTIFUNGAL ACTIVITY AND PROTOPLAST FORMATION BY CHITINASE PRODUCED FROM Bacillus licheniformis NK-7.

Author

VERMA, Kumkum and GARG, Neelam

Subjects
*BACILLUS licheniformis, *CHITINASE, *YEAST extract, *ION sources, *BACTERIAL cultures, *ASPERGILLUS niger
Abstract

Extracellular chitinase was produced by a chitinolytic bacterium, Bacillus licheniformis NK-7, under submerged fermentation. Maximum chitinase production (398.36 U/mL) was obtained when fermentation was carried out at 30°C for 72 h using 1500 µL of 24 h old bacterial culture in a 50 mL minimal salt medium (pH-7) amended with chitin (0.5% w/v; pH-6) under 150 rpm agitation rate. Supplementation of additional carbon, nitrogen and metal ion sources such as lactose, yeast extract and manganese (Mn2+) respectively increased the chitinase production. Further, B. licheniformis NK-7 chitinase demonstrated antifungal activity against Fusarium sp., Curvularia sp. and Aspergillus niger. Protoplasts were also isolated from Aspergillus niger by the action of chitinase produced from B. licheniformis NK-7. [ABSTRACT FROM AUTHOR]

Published
2022

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