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Terahertz Technology is a promising newer technology for various applications in wireless and radar communication namely tracking and detecting radar targets. The challenging aspect of radar transmitters in the target detection process is spurious harmonic signals that affect the communication path between radar transceivers. The spurious signal can be neglected by a strong filtering method. Filtering is vital in radar transmission to avoid high spurious emission level signals. Low pass filtering at terahertz frequency range (LPFT) in microstrip structure defined in the chapter analysis to avoid the harmonics above the cut-off frequency. In this chapter, the analysis part of microstrip structured LPFT is implemented under finite difference time domain analysis at (0.3 THz to 0.5 THz) cut-off frequency. Finite difference time domain (FDTD) is the three-dimensional approach commonly used for the analysis in higher frequency applications. In this FDTD method, Maxwell equation's partial derivatives are centred to finite frequency by discretization. LPFT 3D-plot is characterized by the signal factors of the input signal, reflected signal, and passed signal concerning time. Scattering parameters|s11| and|s21| are characterized by frequency and magnitude plots with an insertion loss of 0.3 dB. Full-wave analysis of LPFT is compared with Chebyshev and Butterworth filter at terahertz cut-off range is implemented. The comparison plot of attenuation versus relative frequency and characteristic impedance versus dielectric constant is shown with FDTD results with good agreement. [ABSTRACT FROM AUTHOR]

Nowadays, according to the Food and Agriculture Organization (FAO), agricultural pests cause severe loss of global crop production every year. In order to eliminate these harmful insect pests, smart agriculture is the best way for farmers to use artificial intelligence techniques integrated with modern information and communication technology. Thus, they can increase the productivity of their crops. The main limiting factor for cultivation, however, is pest and disease infections. It is not possible to eradicate these diseases, but they can be controlled and monitored to minimize their impact. Crop diseases can be rapidly diagnosed by using automated image identification systems. An accurate identification system requires the extraction of features from images. To identify the samples, we used three different classes of machine learning: a support vector machine (SVM), a random forest (RF), and an artificial neural network (ANN). In addition, our deep learning method outperforms other previous recognition methods, namely Single Shot Multi-Box Detector (SSD), which increases the accuracy and multi-directional way of pest detection. Our results contribute to the use of machine learning in agriculture literature and have implications for research in deep learning and practical implications. [ABSTRACT FROM AUTHOR]

The age of technology has introduced integrated wearables into the area of driving to prevent accidents. A smart device is fitted easily into any moving vehicle, which renders a convenient option regarding the activities of the drivers such as abnormality, health condition, and sleep information. This research study discusses two types of outcomes that are enabled according to the situations of the input provided to the BPNN (Back Propagation Neural Network). At first, when an anomaly is predicted within the vehicle, such as the unusual behaviour of the driver, then it is reported through the help of IoT sensors installed inside the vehicle. The BPNN communicates the relevant signal to the driver assistance system to gain control over the vehicle and safely stop it at an appropriate place on the road. The second type of outcome discussed is when an anomaly is explored on the road when a vehicle moves in a heedless way. In such cases, the nearby vehicles are alerted and in the case of an accident are reported, the signals are sent to the local police officials. These suggested instructions perhaps minimize the damage and decrease the rate of mortality every year because of road accidents. Appropriate actions are taken by the model in averting the occurrence of accidents using cloud AI. The performance of the proposed system is assessed based on its accuracy rate. [ABSTRACT FROM AUTHOR]

Abnormal accumulation of acyl-CoA cholesterol acyltransferase-1 (ACAT-1) mediated cholesterol ester has been shown to contribute to cancer progression in various cancers including leukemia, glioma, breast, pancreatic and prostate cancers. However, the significance of ACAT-1 and cholesterol esters (CE) is relatively understudied in ovarian cancer. In this in vitro study, we assessed the expression and contribution of ACAT-1 in ovarian cancer progression. We observed a significant increase in the expression of ACAT-1 and CE levels in a panel of ovarian cancer cell lines (OC-314, SKOV-3 and IGROV-1) compared to primary ovarian epithelial cells (normal controls). To confirm the tumor promoting capacity of ACAT-1, we inhibited ACAT-1 expression and activity by treating our cell lines with an ACAT inhibitor, avasimibe, or by stable transfection with ACAT-1 specific short hairpin RNA (shRNA). We observed significant suppression of cell proliferation, migration and invasion in ACAT-1 knockdown ovarian cancer cell lines compared to their respective controls (cell lines transfected with scrambled shRNA). ACAT-1 inhibition enhanced apoptosis with a concurrent increase in caspases 3/7 activity and decreased mitochondrial membrane potential. Increased generation of reactive oxygen species (ROS) coupled with increased expression of p53 may be the mechanism(s) underlying pro-apoptotic action of ACAT-1 inhibition. Additionally, ACAT-1 inhibited ovarian cancer cell lines displayed enhanced chemosensitivity to cisplatin treatment. These results suggest ACAT-1 may be a potential new target for the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]

Objective: There is an urgent need to substantiate scientifically the roles of contemporary and native medicinal practices. This exercise will open up a new class of drugs which could be cheap, easily affordable, and with less or no side effects. Moringa oleifera or drumstick is known to a wonder herbal plant and most of its parts are used to cure various ailments. The present study deals with the gas chromatography-mass spectrometry (GC-MS) analysis of M. oleifera seeds and to understand the molecules present therein. Methods: GC-MS was performed on the water extract of the dried seeds of the plant by standard procedures. Results: Some important biomolecules such as 2,5-dimethyl-4-hydroxy-3(2H)-furanone, p-cresol, l-Norvaline, N-ethoxycarbonyl-, nonyl ester, Pentanoic acid, 2-hydroxy-, ethyl ester, 1H-1,2,4-Triazole-1-carboxamide, 5-acetylamino- 3-amino-, Ethyl 2-hydroxybenzyl sulfone, beta-l-Arabinopyranoside, methyl, tetradecanoic acid, 10,13-dimethyl-, methyl ester, cyclohexane, 1-isopropyl-1-methyl-, decanoic acid, 2-methyl-, 2-Methylthiolane, S,S-dioxide, 4-Methyl-2,4- bis(4'-trimethylsilyloxyphenyl)pentene-, 2,4(1H, 3H)-Pyrimidinedione, 6-iodo-5-methyl-, 2-Fluoro-6-trifluoromethylbenzoic acid, and 4-nitrophenyl ester have medicinal roles comparable with the various medicinal activities of Moringa seed water extract. Conclusion: It is concluded that the Moringa seed is a potential candidate for drug designing for many diseases. [ABSTRACT FROM AUTHOR]

The design of controllers for solar energy harvesting systems plays the key role in deciding the efficiency of the energy utilized. In applications of energy scavenging, the harvested power is consumed for about 70% in the operation of the converter and controller blocks before being supplied to the load. The proposed optimal Field Programmable Gate Array based Load Predictive Maximum Power Point Tracking (LP_MPPT) Digital Controller is used for low power, speedy, and decisive energy scavenging systems for a long run in applications of wireless or remote sensing or portable. LP_MPPT helps to predict the need of the load and generate the converter output as buck/boost/buck_boost mode power in comparison to the input from solar panels for every clock cycle. The power consumption of the controller section is comparatively reduced as the buck_boost mode of operation utilizes the no operation state. The proposed methodology is simulated and implemented using Xilinx ISE Design Suite 12.1 which is supported by the family SPARTAN 3E. The simulation results thus obtained show an increase in efficiency (94.3%) with trade off factors, namely, speed and area. [ABSTRACT FROM AUTHOR]

Endometrial cancer (EC) is the most prevalent gynecological malignancy. Abnormal accumulation of sterol-O-acyl transferase 1 (SOAT1) and SOAT1-mediated cholesterol ester (CE) contributes to cancer progression in various malignancies, including ovarian cancer. Therefore, it was hypothesized that similar molecular changes may occur in EC. The present study aimed to evaluate the diagnostic and/or prognostic potential of SOAT1 and CE in EC by: i) Determining SOAT1 and CE levels in plasma, peritoneal fluid and endometrial tissue from patients with EC and control subjects; ii) performing receiver operating characteristic curve analysis to determine diagnostic performance; iii) comparing SOAT1 and CE expression to that of the tumor proliferation marker Ki67; and iv) assessing the association between SOAT1 expression and survival. Enzyme-linked immunosorbent assay was used to determine the levels of SOAT1 protein in tissue, plasma and peritoneal fluid. The mRNA and protein expression levels of SOAT1 and Ki67 in tissues were detected by reverse transcription-quantitative polymerase chain reaction and immunohistochemistry, respectively. CE levels were determined colorimetrically in plasma and peritoneal fluid. SOAT1-associated survival data from the cBioPortal cancer genomics database were used to assess prognostic relevance. The results revealed that SOAT1 and CE levels were significantly elevated in tumor tissue and peritoneal fluid samples collected from the EC group. By contrast, the plasma levels of SOAT1 and CE in the EC and control groups were similar. Significant positive associations between CE and SOAT1, SOAT1/CE and Ki67, and SOAT1/CE and poor overall survival in patients with EC suggested that SOAT1/CE may be associated with malignancy, aggressiveness and poor prognosis. In conclusion, SOAT1 and CE may serve as potential biomarkers for prognosis and target-specific treatment of EC. [ABSTRACT FROM AUTHOR]

Previously, Bithionol (BT) was shown to enhance the chemosensitivity of ovarian cancer cell lines to cisplatin treatment. In the present study, we focused on the anti-tumor potential of the BT-paclitaxel combination when added to a panel of ovarian cancer cell lines. This in vitro study aimed to 1) determine the optimum schedule for combination of BT and paclitaxel and 2) assess the nature and mechanism(s) underlying BT-paclitaxel interactions. The cytotoxic effects of both drugs either alone or in combination were assessed by presto-blue cell viability assay using six human ovarian cancer cell lines. Inhibitory concentrations to achieve 50% cell death (IC50) were determined for BT and paclitaxel in each cell line. Changes in levels of cleaved PARP, XIAP, bcl-2, bcl-xL, p21 and p27 were determined via immunoblot. Luminescent and colorimetric assays were used to determine caspases 3/7 and autotaxin (ATX) activity. Cellular reactive oxygen species (ROS) were measured by flow cytometry. Our results show that the efficacy of the BT-paclitaxel combination depends upon the concentrations and sequence of addition of paclitaxel and BT. Pretreatment with BT followed by paclitaxel resulted in antagonistic interactions whereas synergistic interactions were observed when both drugs were added simultaneously or when cells were pretreated with paclitaxel followed by BT. Synergistic interactions between BT and paclitaxel were attributed to increased ROS generation and enhanced apoptosis. Decreased expression of pro-survival factors (XIAP, bcl-2, bcl-xL) and increased expression of pro-apoptotic factors (caspases 3/7, PARP cleavage) was observed. Additionally, increased expression of key cell cycle regulators p21 and p27 was observed. These results show that BT and paclitaxel interacted synergistically at most drug ratios which, however, was highly dependent on the sequence of the addition of drugs. Our results suggest that BT-paclitaxel combination therapy may be effective in sensitizing ovarian cancer cells to paclitaxel treatment, thus mitigating some of the toxic effects associated with high doses of paclitaxel. [ABSTRACT FROM AUTHOR]

Background: Combination drug therapy appears a promising approach to overcome drug resistance and reduce drug-related toxicities in ovarian cancer treatments. In this in vitro study, we evaluated the antitumor efficacy of cisplatin in combination with Bithionol (BT) against a panel of ovarian cancer cell lines with special focus on cisplatin-sensitive and cisplatin-resistant cell lines. The primary objectives of this study are to determine the nature of the interactions between BT and cisplatin and to understand the mechanism(s) of action of BT-cisplatin combination.Methods: The cytotoxic effects of drugs either alone or in combination were evaluated using presto-blue assay. Cellular reactive oxygen species were measured by flow cytometry. Immunoblot analysis was carried out to investigate changes in levels of cleaved PARP, XIAP, bcl-2, bcl-xL, p21 and p27. Luminescent and colorimetric assays were used to test caspases 3/7 and ATX activity.Results: The efficacy of the BT-cisplatin combination depends upon the cell type and concentrations of cisplatin and BT. In cisplatin-sensitive cell lines, BT and cisplatin were mostly antagonistic except when used at low concentrations, where synergy was observed. In contrast, in cisplatin-resistant cells, BT-cisplatin combination treatment displayed synergistic effects at most of the drug ratios/concentrations. Our results further revealed that the synergistic interaction was linked to increased reactive oxygen species generation and apoptosis. Enhanced apoptosis was correlated with loss of pro-survival factors (XIAP, bcl-2, bcl-xL), expression of pro-apoptotic markers (caspases 3/7, PARP cleavage) and enhanced cell cycle regulators p21 and p27.Conclusion: In cisplatin-resistant cell lines, BT potentiated cisplatin-induced cytotoxicity at most drug ratios via enhanced ROS generation and modulation of key regulators of apoptosis. Low doses of BT and cisplatin enhanced efficiency of cisplatin treatment in all the ovarian cancer cell lines tested. Our results suggest that novel combinations such as BT and cisplatin might be an attractive therapeutic approach to enhance ovarian cancer chemosensitivity. Combining low doses of cisplatin with subtherapeutic doses of BT can ultimately lead to the development of an innovative combination therapy to reduce/prevent the side effects normally occurring when high doses of cisplatin are administered. [ABSTRACT FROM AUTHOR]

6-Phenylazo-3-( p -tolyl)-2 H -chromen-2-one 4 was prepared and characterized by IR, 1 H, and 13 C NMR spectral studies. The optimized structure of the chromen-2-one 4 was investigated by the Gaussian 03 B3LYP density functional method calculations at 6-31G(d,p) basis set. The gauge-independent atomic orbital (GIAO) 13 C and 1 H chemical shift calculations for the synthesized chromen-2-one in CDCl 3 were also made by the same method. The computed IR frequencies of the chromen-2-one and the corresponding vibrational assignments were analyzed by means of potential energy distribution (PED%) calculation using vibrational energy distribution analysis (VEDA) program. The first order hyperpolarizability ( β tot ), polarizability ( α ) and dipole moment ( μ ) were calculated using 6-311G(d,p) basis set and the nonlinear optical (NLO) properties are also addressed theoretically. Stability of the chromen-2-one 4 molecule has been analyzed by calculating the intramolecular charge transfer using natural bond order (NBO) analysis. The molecular electrostatic potentials, HOMO–LUMO energy gap and geometrical parameters were also computed. Topological properties of the electronic charge density in chromen-2-one 4 were analyzed employing the Bader’s Atoms in Molecule (AIM) theory which indicated the presence of intramolecular hydrogen bond in the molecule. [ABSTRACT FROM AUTHOR]

Background Drug resistance is a cause of ovarian cancer recurrence and low overall survival rates. There is a need for more effective treatment approaches because the development of new drug is expensive and time consuming. Alternatively, the concept of 'drug repurposing' is promising. We focused on Bithionol (BT), a clinically approved anti-parasitic drug as an anti-ovarian cancer drug. BT has previously been shown to inhibit solid tumor growth in several preclinical cancer models. A better understanding of the anti-tumor effects and mechanism(s) of action of BT in ovarian cancer cells is essential for further exploring its therapeutic potential against ovarian cancer. Methods The cytotoxic effects of BT against a panel of ovarian cancer cell lines were determined by Presto Blue cell viability assay. Markers of apoptosis such as caspases 3/7, cPARP induction, nuclear condensation and mitochondrial transmembrane depolarization were assessed using microscopic, FACS and immunoblotting methods. Mechanism(s) of action of BT such as cell cycle arrest, reactive oxygen species (ROS) generation, autotaxin (ATX) inhibition and effects on MAPK and NF-κB signalling were determined by FACS analysis, immunoblotting and colorimetric methods. Results BT caused dose dependent cytotoxicity against all ovarian cancer cell lines tested with IC 50 values ranging from 19 µM - 60 µM. Cisplatin-resistant variants of A2780 and IGROV-1 have shown almost similar IC50 values compared to their sensitive counterparts. Apoptotic cell death was shown by expression of caspases 3/7, cPARP, loss of mitochondrial potential, nuclear condensation, and up-regulation of p38 and reduced expression of pAkt, pNF-κB, pIκBα, XIAP, bcl-2 and bcl-xl. BT treatment resulted in cell cycle arrest at G1/M phase and increased ROS generation. Treatment with ascorbic acid resulted in partial restoration of cell viability. In addition, dose and time dependent inhibition of ATX was observed. Conclusions BT exhibits cytotoxic effects on various ovarian cancer cell lines regardless of their sensitivities to cisplatin. Cell death appears to be via caspases mediated apoptosis. The mechanisms of action appear to be partly via cell cycle arrest, ROS generation and inhibition of ATX. The present study provides preclinical data suggesting a potential therapeutic role for BT against recurrent ovarian cancer. [ABSTRACT FROM AUTHOR]

Introduction: In the present study, hexane, chloroform, ethyl acetate, butanol and methanol extracts of fruits of Muntingia calabura L (Elaeocarpaceae) were examined total phenolics (TP) and in vitro antioxidative capacity. Methods: For the determination of total phenolics (TP) and in vitro antioxidative capacity, established assay methods such as 1, 1 - diphenyl - 2- picryl hydroxyl (DPPH) radical assay, reducing power, ferric ion chelating assay, superoxide anion, and nitric oxide scavenging activity assays were used with reference to synthetic antioxidant butyl hydroxyl toluene (BHT). One way analysis of variance (ANOVA) and Duncan's Multiple Range Test (DMRT) were carried out. Results: The TP ranged from 1486 ± 0.028 mg GAE /100 of fresh mass (FW) to 358 ± 0.020 mg GAE/100 of fresh mass (FW). The data obtained from the study showed high levels of antioxidant activity of the fruit extracts. Conclusion: From the findings, it was observed that there was a well correlation between antioxidant activity and total phenolic/flavonoid contents. These results may be useful to further analyze wild edible fruits that contain most antioxidant activity in order to identify the active constituents. [ABSTRACT FROM AUTHOR]

Abstract: Effects of UV-A, UV-B and their combination on spore viability and larvicidal activity of an indigenous isolate of Bacillus sphaericus Neide, ISPC-8 were studied under laboratory conditions. The UV sensitivity of ISPC-8 was compared with standard strain 1593 and larvicidal activity was tested against third instar larvae of mosquito, Culex quinquefasciatus Say. No significant adverse effects on viability as well as larvicidal activity of both strains were observed when spores were exposed to UV-A for 6h. However, exposure to UV-B for a few minutes adversely affected the spore viability as well as larvicidal activity and this adverse effect was more pronounced on spore viability. In both strains about 50% larvicidal activity was retained after exposure of the spores to UV-B for 8h. However, spore viability at this exposure of time was drastically reduced to 2.5% in ISPC-8 and 0.3% in 1593. The spore viability and larvicidal activity patterns were found to be similar to UV-B treatment when spores were exposed to a combination of UV-A and UV-B. Our study hence, shows the adverse effect of UV radiation on ISPC-8 and 1593 indicating the need to incorporate eco-friendly UV protectants in formulations so that the efficacy of biopesticides based on these entomopathogens can be prolonged under field conditions, especially in tropical countries. [Copyright &y& Elsevier]

Polymer blends of silicone polymer with polymethylmethacrylate (PMMA) have been prepared by solution blending, and their miscibility has been investigated by using physical techniques like viscosity, refractive index, density and ultrasonic velocity for blend solutions at different percentages of the blend composition. All the measurements are made at 30°C. The results are discussed. [ABSTRACT FROM AUTHOR]

Nitrogen Dioxide (NO2) is a product of high-temperature combustion and an environmental oxidant of concern. We have recently reported that early changes in NO2-exposed human bronchial epithelial cells are causally linked to increased generation of proinflammatory mediators, such as nitric oxide/nitrite and cytokines like interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-8. The objective of the present in vitro study was to further delineate the cellular mechanisms of NO2-mediated toxicity, and to define the nature of cell death that ensues upon exposure of normal human bronchial epithelial (NHBE) cells to a brief high dose of NO2. Our results demonstrate that the NHBE cells undergo apoptotic cell death during the early post-NO2 period, but this is independent of any significant increase in caspase-3 activity. However, necrotic cell death was more prevalent at later time intervals. Interestingly, an increased expression of HO-1, a redox-sensitive stress protein, was observed in NO2-exposed NHBE cells at 24 h. Since neutrophils (PMNs) play an active role in acute lung inflammation and resultant oxidative injury, we also investigated changes in human PMN–NHBE cell interactions. As compared to normal cells, increased adhesion of PMNs to NO2-exposed cells was observed, which resulted in an increased NHBE cell death. The latter was also increased in the presence of IL-8 and TNF-α + interferon (IFN)-γ, which correlated with upregulation of intercellular adhesion molecule-1 (ICAM-1). Our results confirmed an involvement of nitric oxide (NO) in NO2-induced cytotoxicity. By using NO synthase inhibitors such as L-NAME and 3-aminoguanidine (AG), a significant decrease in cell death, PMN adhesion, and ICAM-1 expression was observed. These findings indicate a role for the L-arginine/NO synthase pathway in the observed NO2-mediated toxicity in NHBE cells. Therapeutic strategies aimed at controlling excess generation of NO and/or inflammatory cytokines may be useful in alleviating NO2-mediated adverse effects on the bronchial epithelium. [ABSTRACT FROM AUTHOR]

The high reactivity of the chloroacetyl group has been exploited for the synthesis of bile acid based first and second generation dendrons with multiple hydroxyl groups. The synthesis involves only a few steps and avoids the use of protecting groups for the terminal hydroxyl groups. These dendritic structures with facially amphiphilic bile acid backbones on the periphery were able to solubilize cresol red, a hydrophilic dye, in a nonpolar solvent. HPLC analysis of the dendrons suggests that hydrophobicity increases with increase in oligomer size, but in each generation, the dendrons with a higher degree of branching are less hydrophobic. [ABSTRACT FROM AUTHOR]

Nitrogen dioxide (NO2) is an environmental oxidant, known to be associated with lung epithelial injury. In the present study, cellular pro-inflammatory responses following exposure to a brief high concentration of NO2 (45 ppm) were assessed, using normal human bronchial epithelial (NHBE) cells as an in vitro model of inhalation injury. Generation and release of pro-inflammatory mediators such as nitric oxide (NO), IL-8, TNF-α, IFN-γ and IL-1β were assessed at different time intervals following NO2 exposure. Effects of a pre-existing inflammatory condition was tested by treating the NHBE cells with different inflammatory cytokines such as IFN-γ, IL-8, TNF-α, IL-1β, either alone or in combination, before exposing them to NO2. Immunofluorescence studies confirmed oxidant-induced formation of 3-nitrotyrosine in the NO2-exposed cells. A marked increase in the levels of nitrite (as an index of NO) and IL-8 were observed in the NO2-exposed cells, which were further enhanced in the presence of the cytokines. Effects of various NO inhibitors combined, with immunofluorescence and Western blotting data, indicated partial contribution of the nitric oxide synthases (NOSs) toward the observed increase in nitrite levels. Furthermore, a significant increase in IL-1β and TNF-α generation was observed in the NO2-exposed cells. Although NO2 exposure alone did induce slight cytotoxicity (<12%), but presence of inflammatory cytokines such as TNF-α and IFN-γ resulted in an increased cell death (28–36%). These results suggest a synergistic role of inflammatory mediators, particularly of NO and IL-8, in NO2-mediated early cellular changes. Our results also demonstrate an increased sensitivity of the cytokine-treated NHBE cells toward NO2, which may have significant functional implications in vivo. [Copyright &y& Elsevier]

Nanomaterials are the key to the success of cutting-edge monitoring and diagnostics systems enabled by flexible electronics that are revolutionizing healthcare systems. Among them, layered 2D-based materials with exceptional qualities including high electrical conductivity, mechanical flexibility, thermal stability, and optical adaptability stand out. These include graphene and transition metal dichalcogenides. This comprehensive examination covers the fabrication and characterisation of these nanomaterials, as well as their immense potential for usage in flexible electronics for biomedical applications. The layered 2D-based nanomaterials have changed the face of healthcare technology by providing examples ranging from wearable health monitoring devices to implantable sensors. This has improved both device performance and user experience. There are many difficulties including biocompatibility and long-term stability while simultaneously addressing moral and legal issues, highlighting the significance of responsible technology development in this game-changing area. The extraordinary characteristics of layered 2D-based nanomaterials are revealed in this study, presenting them as key features in developing flexible electronics with the potential to take patient care and diagnostics to whole new levels. [ABSTRACT FROM AUTHOR]

The article presents a case study of a 56-year-old man who was a former smoker and developed a giant cell carcinoma of the lung. He was presented with a cough and hemoptysis for two months. His chest skiagram and computerized tomography (CT) chest examination showed opacity in his right upper lobe, decreased air entry in his right lung apex, and mixed density mass lesion in the right upper lobe. His condition deteriorated and he succumbed due to the disease nine months after surgery.

Deals with a study which explored the inheritance of flower color in chickpea. Materials and methods; Results and discussion.

Costus igneus, common name Fiery Costus or Spiral Flag, is a species of herbaceous plant in the Costaceae family. It is cultivated in India for its use in traditional medicine especially for diabetes. The present study was carried out to determine the mechanism of anti-inflammatory action of β-amyrin isolated from the leaves of Costus igneus ( C. igneus) using carrageenan-induced rat model and LPS-induced human peripheral blood mononuclear cells (hPBMCs) in vitro model. The differential fractionation of leaves of Costus igneus showed maximum percentage inhibition of paw edema at a dose of 100 mg/kg body weight in methanolic extract (MEC). MEC elicited significant anti-inflammatory effect by inhibiting cyclooxygenase (COX), lipoxygenase (LOX), myeloperoxidase (MPO) and nitric oxide synthase (NOS) activities in monocytes when compared to carrageenan control. The effect of MEC was more pronounced than standard drug Diclofenac (20 mg/kg body weight). After fractionation of MEC using various solvents such as chloroform, hexane, ethyl acetate and butanol, the mechanism of anti-inflammatory effect of chloroform extract (CEC) of MEC was evaluated since it showed maximum beneficial effect at a dose of 50 mg/kg BW Treatment of carrageenan-induced rats with CEC exerted significantly decreased COX-2, MPO, and NOS activities when compared to carrageenan-induced rats. By the partial purification of CEC by liquid-liquid partition chromatography, TLC, mass, IR and NMR spectroscopy, the active component β-amyrin was isolated. Significant decrease in edema was observed by the administration of β-amyrin in a dose-dependent manner and 100 µg of β-amyrin showed 97 % in carrageenan-induced paw edema in rats. Treatment with β-amyrin significantly inhibited PGE2, IL-6 secretion, and NF-κB activation in a concentration-dependent manner on LPS-induced hPBMCs. Thus, β-amyrin, an active component isolated from C. igneus, serves as a promising and expanding platform for treatment of various inflammatory disorders. [ABSTRACT FROM AUTHOR]

Sustained-release biopolymer based formulations for an indigenous Bacillus sphaericus Neide ISPC-8 strain were developed and evaluated under laboratory conditions. These formulations were assessed for residual activity, response to UV-B radiation and shelf-life. The mosquitocidal activity of tested formulations showed a varied residual activity pattern against Culex quinquefasciatus Say larvae. The residual activity of unformulated spore-crystal suspension drastically decreased after 4 days whereas the formulated preparations persisted until 28 days. All the formulations except sodium alginate-based formulation showed more than 95% mortality within 48 h. Although the sodium alginate-based formulation showed delayed initial mortality, it persisted for a longer period of time (42 days). Based on the persistence studies, promising formulations based on sodium alginate, gelatin and acacia gum were selected for further evaluation. The respective LC values of these three formulations were 0.085, 0.117, and 0.196 mg/l against C. quinquefasciatus after 48 h. The biopolymers combined with Congo red protected the spore-crystal moiety from UV-B radiation. These formulations were found to be stable up to 18 months when stored at room temperature. Thus, these biopolymers could serve as suitable ingredients for development of biopesticide based on B. sphaericus ISPC-8. [ABSTRACT FROM AUTHOR]

Oral administration of flavonoids extracted from unripe fruits of Musa paradisiaca showed significant hypolipidemic activities in male rats (Sprague Dawley strain) at a dose of 1 mg/100 g body weight (BW)/day. Concentrations of cholesterol, phospholipids, free fatty acids, and triglycerides showed significant decrease in the serum, liver, kidney, and brain of experimental animals. HMG CoA reductase activity was found to be enhanced, while activities of glucose-6-phosphate dehydrogenase and malate dehydrogenase were significantly reduced. Activities of lipoprotein lipase and plasma LCAT showed significant enhancement. A significant increase in the concentrations of hepatic and fecal bile acids and fecal neutral sterols was also observed indicating a higher rate of degradation of cholesterol. The present study indicates that although there is an increase in the rate of synthesis of cholesterol in the liver, the process of degradation exceeds the rate of synthesis. [ABSTRACT FROM AUTHOR]

Abstract: The UV protectant properties of 26 natural and synthetic compounds were investigated for a biopesticide based on an indigenously isolated strain (ISPC-8) of Bacillus sphaericus Neide. In initial screening, spores of ISPC-8 with 0.1% (w/w for solid and v/w for liquid materials) concentration of different compounds were exposed to UV-B radiation (4.9×105 J/m2) for 6h and their spore viability and larvicidal activity were studied. The larvicidal activity was evaluated against third-instar larvae of Culex quinquefasciatus Say. There was a complete loss of spore viability (1.4% viable spores) and partial reduction in larvicidal activity (57.7% of original activity) after exposure of spores to UV-B for 6h. However, spore viability as well as larvicidal activity protected significantly when spores were mixed with different compounds before exposing them to UV-B. Among the different compounds tested benzaldehyde, congo red, para-aminobenzoic acid (PABA) and cinnamaldehyde were found to be promising in protecting the spores from UV-B radiation. The presence of binary toxins (41.9kDa and 51.4kDa) in protected and unprotected samples were examined by SDS–PAGE. The binary toxin bands disappeared in unprotected spores after 24h of exposure to UV-B, whereas toxin bands were distinctly visible when spores with benzaldehyde and cinnamaldehyde were exposed to UV-B for 96h and 120h, respectively. Congo red and PABA were found to be most effective in protecting binary toxins even after 168h of exposure to UV-B. Incorporation of these promising UV protectant compounds in biopesticides would help in protecting the spores from the adverse effects of UV radiation and prolong the persistence of biopesticides under field conditions. [Copyright &y& Elsevier]

Objective: There is an urgent need to prove the authenticity of Ayurvedic medicines in the light of modern scientific knowledge. Methods: The present study deals with the gas chromatography-mass spectrometry (GC-MS) analysis of one herbal oil, Kunthalakanti Thailam, to understand the molecules present there in. Kunthalakanti Thailam was bought from a standard Ayurvedic vendor from Chennai. It was processed and GC-MS analysis was done by standard procedures. Results: The GC-MS report indicated the presence of mostly fatty acids and their esters which have potent antioxidant and antiallerg ic properties such as N-Hexadecanoic acid, 2-Methyl-3-93-methyl-but-2-enyl)-enyl)-oxetane, 3, 7-decadien-2-one, and 10-(3,3-dimethoxyloxiran dimethyl-, (E, E)-, +/- which have medicinal activities which help in keeping the homeostasis of the scalp and maintain good health to hair follicles and hair. Some important biomolecules have medicinal properties supporting the validity of skin treatment with this oil. Conclusions: The presence of these biomolecules having medicinal properties supports the validity of Kunthalakanti Thailam as skin and hair health treatment. [ABSTRACT FROM AUTHOR]

Introduction: The available modern molecular drugs for the therapy of Rheumatoid arthritis are beset with several side effects and alternative drugs are urgently needed. The present investigation was performed to evaluate the anti-arthritic activity of Sahacharadi Kashayam (SK), an Ayurvedic formulation, against Freund's complete adjuvant (CFA) - induced arthritis in rats. Methods: In this experimental trial, SK was administered at doses of 0.5, 1.0 and 1.5 ml/kg body weight orally to adjuvant (CFA) induced arthritic rats. The anti-arthritic activity was evaluated by using paw volume, haematological parameters and arthritic biomarkers. The efficacy of the Kashayam was compared with the standard Leflunomide (10 mg/kg) drug. Results: Significant reduction in paw volume and thickness by SK (0.5 ml dose) has been found and there was considerably improvement in haematological parameters and arthritic markers in CFA rats till 14 days. After 14th day SK treatment with doses (1.0 and 1.5ml), however, reoccurrence of inflammation and pathological changes were observed in rats. Conclusion: The study clearly indicated the anti-arthritic role of SK. Future studies, however, are warranted to provide a new approach in relation to the therapeutic dose and treatment period of SK which may eventually lead to the development of a new category of the anti-arthritic agent. [ABSTRACT FROM AUTHOR]

Objective: The present study deals with three antioxidant assays for one Ayurvedic medicine, Mahanarayana Thailam which is used to treat diseases such as arthritis, paralysis, facial palsy, hearing loss, tremors, wasting of muscles, insanity, oligospermia, female infertility, headache, psychosis, humpback, and eye diseases. Materials and Methods: 2,2’-azino-bis-3- ethylbenzthiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant potential (FRAP), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays were conducted following standard protocols. Results: Mahanarayana Thailam showed very good antioxidant potential at very low concentrations in FRAP assay as compared to ABTS and DPPH assays. Conclusion: These results clearly indicate the medicinal efficacy of Mahanarayana Thailam as a very good antioxidant formula. [ABSTRACT FROM AUTHOR]

Objective: The present study deals with the three antioxidant assays, namely, 2, 2’-azino-bis (3-ethylbenzothiazoline6-sulfonic acid) (ABTS), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) by standard protocols for one Ayurvedic medicine, Drakshadi Kashayam which is used to cure a number of ailments. Materials and Methods: Drakshadi Kashayam was procured from standard Ayurvedic vendor at Chennai, India, and was subjected to GC-MS analysis following standard protocol. Results: It was observed that among the three assays, Drakshadi Kashayam indicated excellent results at very low concentrations in FRAP assay as compared to ABTS and DPPH assays which indicated moderate antioxidant activities of the medicine. Conclusion: These results clearly indicate the antioxidant potentials of Drakshadi Kashayam proving its medicinal efficacy. [ABSTRACT FROM AUTHOR]

Objective: Verification of the herbal plants by latest scientific tools toward understanding the molecular mechanism underlying their medicinal role is the need of the hour. Methods: Gas chromatography-mass spectrometry study of water extracts of the leaves of one herbal plant, Aristolochia indica was undertaken by standard procedures and its medicinal activity was verified with the biomolecules present in it. Results: The presence of some important biomolecules such as 2-propenoic acid, ethenyl ester, oxalic acid, cyclobutyl ethyl ester, phosphoric acid, 2-chloroethenyl dimethyl ester, pentanoic acid, 2-methylcyclohexyl ester, cis-, butylphosphonic acid, butyl cyclohexylmethyl ester, octadecanoic acid, 2,3-dihydroxypropyl ester, acetic acid, cesium salt and 2-Fluoro-6-trifluoromethylbenzoic acid, 4-nitrophenyl ester, and some others indicate represents the medicinal roles of this plant as is reported ethnopharmacologically. Conclusions: The results and discussion above clearly indicate that the various molecules present in A. indica leaf extracts do have medicinal roles ascribed to it and further work in this regard is on. [ABSTRACT FROM AUTHOR]

Introduction: The present work deals with the gas chromatography–mass spectrometry (GC–MS) analysis of Ksheerabala Thailam which is an Ayurvedic oil used for the treatment of Vata disorders such as neuromuscular pains, sciatica, spondylitis, paralysis, myalgia, for nerve regeneration, cataract, earache, and headache. Materials and Methods: Ksheerabala Thailam was bought from a standard Ayurvedic vendor at Chennai and subjected to GC–MS analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC–MS profile were screened for their various medicinal roles using Dr. Duke’s phytochemical and ethnobotanical data and other data. Results: GC–MS profile of the Ksheerabala Thailam indicated the presence of important biomolecules such as n-Hexadecanoic acid, 15-Hydroxypentadecanoic acid, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, (3-Fluorophenyl)carbamic acid, 2-isopropyl-5-methylphenyl ester, Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester, Hexadecanoic acid, 2-(octadecyloxy)ethyl ester,. gamma.-Tocopherol, and gamma.-Sitosterol; these molecules do have medicinal roles which relate to that of Ksheerabala Thailam. Conclusions: It is concluded that Ksheerabala Thailam, which is an important Ayurvedic medicine, does contain some very important molecules showing its efficacy. Further research is required for a better understanding of the medicinal roles of Ksheerabala Thailam. [ABSTRACT FROM AUTHOR]

Introduction: This study aims to know the types of biomolecules present in Eladi Kera Thailam and to correlate their possible medicinal roles supporting Eladi Kera Thailam’s role as medicine. Methods: Eladi Kera Thailam was bought from a standard Ayurvedic vendor at Chennai and subjected to gas chromatography/mass spectrometry (GC/MS) analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC/MS profile were screened for their various medicinal roles using Dr. Duke’s phytochemical and ethnobotanical data and other data. Results: The presence of some important biomolecules such as dodecanoic acid, 2,3-dihydroxypropyl ester, dodecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, octadecanoic acid, 2,3-dihydroxypropyl ester, and dodecanoic acid, 1,2,3-propanetriyl ester does have medicinal roles which contribute to Eladi Kera Thailam as a medicine for skin ailments. Conclusions: The GC/MS profile indicated the presence of some important biomolecules which have medicinal properties contributing to the role of Eladi Kera Thailam as a skin cure medicine. The study is a step in the direction of understanding the molecular mechanism of contemporary and alternative medicines. [ABSTRACT FROM AUTHOR]

Introduction: The present study deals with the gas chromatography–mass spectrometry (GC–MS) analysis of an Ayurvedic oil Triphaladi Thailam, which is used to treat spilt hair, headache, sinusitis, and rhinitis and also for diseases related to neck, eyes, ear, and throat and to understand the molecular roles of this medicine. Methods: Triphaladi Thailam was bought from a standard Ayurvedic vendor at Chennai and subjected to GC–MS analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC–MS profile were screened for their various medicinal roles using Dr. Duke’s phytochemical and ethnobotanical data and other data. Results: GC–MS profile of the Triphala Rasayana indicated the presence of important biomolecules such as 2-[4-methyl-6-(2,6,6-trimethylcyclohex-1-enyl)hexa-1,3,5-, á-Sitosterol, 1-Monolinoleoylglycerol trimethylsilyl ether, 1H-2,8a-Methanocyclopenta[a]cyclopropa[e]cyclodecen-11-one, and oleic acid. Conclusions: It is concluded that Triphala, which an age-old multipurpose folklore medicine does have a scientific efficacy. Further research is required for a better understanding of the medicinal roles of Triphaladi Thailam. [ABSTRACT FROM AUTHOR]

Objective: The present study deals with the gas chromatography–mass spectrometry (GC–MS) analysis of an anti-obesity Ayurvedic medicine, Lohasava. Methods: Lohasava was bought from a standard Ayurvedic vendor at Chennai and subjected to GC–MS analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC–MS profile were screened for their various medicinal roles using Dr. Duke’s phytochemical and ethno-botanical data and other data. Results: The GC–MS profile of Lohasava indicated the presence of important biomolecules such as butanoic acid, 3-hydroxy-, ethyl ester, phenylethyl alcohol, ethyl hydrogen succinate, thymol, 4-hydroxy-2-methylacetophenone, hydrocinnamic acid, 2,6-dimethoxyphenyl-beta-phenylpropionate, bisphenol C, benzeneethanamine, 2,5-dimethoxy-alpha.,4-dimethyl-and piperine. Conclusions: It is concluded that Lohasava does have a scientific efficacy toward its claimed role as a medicine in Ayurveda. Further research is required for a better understanding of the medicinal roles of Lohasava. [ABSTRACT FROM AUTHOR]

Introduction: The present work deals with the gas chromatography (GC)–mass spectrometry (MS) analysis of Drakshadi Kashayam, which is used in the treatment of Panduroga (anemia), jaundice, and diseases caused due to imbalance in Pitta (bile) consistency. Methods: Drakshadi Kashayam was bought from a standard Ayurvedic vendor at Chennai and subjected to GC–MS analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC–MS profile were screened for their various medicinal roles using Dr. Duke’s phytochemical and ethnobotanical data and other data. Results: GC–MS profile of Drakshadi Kashayam indicated the presence of important biomolecules such as carbonic acid, pentyl phenyl ester, bisphenol C, histamine, N-benzoyl-2-cyano-, o-Methoxy-.alpha.-phenethylamine, benzenethanamine, 3,4-dimethoxy-.alpha.-methyl-, cis-Z-.alpha.-Bisabolene epoxide, and 1,3-Dimethoxy-5-(1-methyl-heptyl)-benzene which have medicinal roles supporting the efficacy of Drakshadi Kashayam as a liver tonic. Conclusions: It is concluded that Drakshadi Kashayam, which is an important Ayurvedic medicine, does contain some very important molecules showing its efficacy. Further research is required for a better understanding of the medicinal roles of this medicine. [ABSTRACT FROM AUTHOR]

Introduction: The present work deals with the gas chromatography–mass spectrometry (GC-MS) analysis of Kutajarishtam which is used in the treatment of diarrhea, dysentery, irritable bowel syndrome, Crohn’s disease, hemorrhoids, etc. Materials and Methods: Kutajarishtam was bought from a standard Ayurvedic vendor at Chennai and subjected to GC-MS analysis by standard procedures. The medicinal roles of the biomolecules indicated in the GC-MS profile were screened for their various medicinal roles using Dr. Duke’s Phytochemical and ethnobotanical data and other data. Results: GC-MS profile of Kutajarishtam indicated the presence of important biomolecules such aso-Acetyl-L-serine, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, 2-Furanethanol., beta.-ethoxy-, Acetic acid, 1-(2-hydroxy-1-methyl-ethyl)-3-methoxymethoxy-2-methyl-propyl ester and Cyclohexan-1,4,5-triol-3-one-1-carboxylic acid which have medicinal roles which correlated well with that of Kutajarishtam. Conclusions: It is concluded that Kutajarishtam, which is an important Ayurvedic medicine, contains some very important molecules showing its efficacy. Further research is required for a better understanding of the medicinal roles of this medicine. [ABSTRACT FROM AUTHOR]

Objective: Anuthailam, an Ayurvedic oil used for the treatment of diseases related to the brain, nose, sinus, eyes, head, neck, shoulders, and arms. Anuthailam is subjected to Gas chromatography–mass spectrometry (GC-MS) analysis to understand the molecules present therein. Materials and Methods: Anuthailam was bought from a standard Ayurvedic vendor at Chennai and subjected to GC-MS analysis by the standard procedures. The medicinal roles of the biomolecules indicated in the GC-MS profile were screened for their various medicinal roles using Dr. Duke’s Phytochemical and ethno-botanical data and other data. Results: GC-MS profile of the Anuthailam indicated the presence of important biomolecules such as 15-Hydroxypentadecanoic acid, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, trans-13-Octadecenoic acid, 9-Octadecenoic acid (Z)-, 2-hydroxy-1-(hydroxymethyl)ethyl ester, 1-Heptatriacotanol., gamma.-Tocopherol, (3-Fluorophenyl)carbamic acid, 2-isopropyl-5-methylphenyl ester, Stigmasterol, Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester, etc. Conclusions: It is concluded that Anuthailam, does have a scientific efficacy toward its claimed role as a medicine in Ayurveda. Further research is required for a better understanding of the medicinal roles of Anuthailam. [ABSTRACT FROM AUTHOR]

Objective: The scientific validation of ethnopharmacologically important herbal plants by latest techniques is necessary to understand their medicinal efficacy. Methods: Gas chromatography-mass spectrometry study of one herbal plant, Dodonaea viscosa var. angustifolia was undertaken by standard procedures and its medicinal activity was verified with the biomolecules present in it. Results: The presence of some important biomolecules such as catechol, N-benzyl-2-phenethylamine, acetic acid, 4-oxocyclohexyl ester, 4-amino-3-mercapto-1,2,4-triazole, 1,1'-methylene-bis(di-2-propenylamine), inositol, alanine, N-methyl-N-methoxycarbonyl-, hexyl ester, 1,3-dioxolane, 2-methyl-2-pentyl-, cyclohexane, 1-methyl-3-(1-methylethenyl)-, cis-, phthalic acid, di(2,3-dimethylphenyl) ester, heptafluorobutyric acid, 2-naphthyl ester, and 2-fluoro-6- trifluoromethylbenzoic acid, 4-nitrophenyl ester has biochemical properties which represent the ethnomedicinal roles of this plant. Conclusions: The presence of these biomolecules having medicinal properties supports the validity of the medicinal roles of D. viscosa var. angustifolia. [ABSTRACT FROM AUTHOR]

Objective: Scientific efficacy evaluation of herbal plants for their medicinal roles is needed to establish their importance in medical science. The present study deals with the gas chromatography-mass spectrometry (GC-MS) analysis of one medicinal plant, Stachytarpheta indica. Methods: The GC-MS study of water extracts of the leaves of one herbal plant, S. indica was undertaken by standard procedures and its medicinal activity was verified with the biomolecules present in it. Results: The presence of some important biomolecules such as sulfurous acid, 2-ethylhexyl hexyl ester, and pentanoic acid has medicinal roles such as acidifier, arachidonic acid inhibitor, and increase aromatic amino acid decarboxylase activity. N-t-Butylpyrrole, 1-hexanol, 4-methyl-, 3-methyl-2-(2-oxopropyl)furan, butanoic acid, 3-methyl-, 3,7-dimethyl-6-octenyl ester, hexadecanoic acid, methyl ester, 11,14,17-eicosatrienoic acid, methyl ester, 2-methylheptanoic acid and 2-propenoic acid, 3-(dimethylamino)-, methyl ester, etc., have very important medicinal roles which correlate well with the medicinal role of the plant. Conclusions: The results and discussion above clearly indicate that the various molecules present in S. indica leaf extracts do have medicinal roles ascribed to it and further work in this regard is on. [ABSTRACT FROM AUTHOR]

Objective: The present study deals with the preliminary phytochemical and gas chromatography-mass spectrometry (GC-MS) analysis of different leaf extracts of one medicinal Carissa carandas. Materials and Methods: The phytochemical and GC- MS study of one medicinal plant, C. carandas was done as per standard protocols to correlate its medicinal activity with the biomolecules present in it. Results: The methanol extract of C. carandas indicated the presence of flavonoids, tannins, saponins, and steroids, whereas flavonoids, alkaloids, saponins, and steroids were present in hexane fraction. Alkaloids, saponins, proteins, and triterpenoids were present in aqueous extract. The GC-MS analysis of hexane and aqueous leaf extracts indicated the presence of some important biomolecules such as pentadecanoic acid, 14-methyl-, methyl ester, 9,12,15-octadecatrienoic acid, (Z, Z, Z)-, Phytol, Bis(2-ethylhexyl) phthalate, Squalene, Octasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15- hexadecamethyl, beta.-Sitosterol, 9,12,15-Octadecatrienoic acid, 2-[(trimethylsilyl)oxy]-1-[[(trimethylsilyl)oxy]methyl] ethyl ester, (Z, Z, Z)-Acetamide, N-methyl-N-[4-(3-hydroxypyrrolidinyl)-2-butynyl]-, 5,8,11,14-Eicosatetraenoic acid, methyl ester, (all-Z)-, and 6-Octadecenoic acid, methyl ester, (Z). Conclusions: The presence of these molecules augurs well with the medicinal properties attributed to this plant ethnobotanical. Further work is in progress toward understanding this plant's role as a medicine. [ABSTRACT FROM AUTHOR]

Aims Non-Tuberculous Mycobacteria ( NTM) are ubiquitous in nature. The data on prevalence of NTM under the RNTCP is scarce. Many NTM species have clinical significance, and hence their identification and speciation are important. Methods and Results It is a cross-sectional study conducted at the five RNTCP accredited culture and drug susceptibility testing ( CDST) laboratory. The culture isolates from AFB positive but Immunochromatographic test negative samples were taken for identification and speciation using HPLC. Of the total 266 isolates only 164 isolates had a second sample received at the laboratory. The speciation was done using HPLC for those isolates. The type of species identified are: 26·8% (44) were Mycobacterium chelonae, 12·8% (21) were Mycobacterium fortuitum, 9% (15) were Mycobacterium gordonae, 9% (15) were Mycobacterium tuberculosis complex, 6·1% (10) were Mycobacterium kansasii, 4·9% (8) were Mycobacterium simiae, 2·4% (4) were Mycobacterium thermophile, 1·2% (2) were Mycobacterium gastri, 0·6% (1) were Mycobacterium scrofulaceum, 0·6% (1) were Mycobacterium avium and 4·9% (8) isolates had chromatogram which was un-interpretable. Conclusion Identification and its speciation of NTM are not routinely done under TB control programme. Since HPLC could identify 95% of isolates belonging to 10 species, the speciation of NTM using HPLC should gain importance in the diagnosis of disease caused by NTM. Significance and Impact of Study NTM are emerging as important causative agents of pulmonary and extra pulmonary disease, the ability to recognize disease caused by NTM and subsequently treat such disease has become increasingly important. The identification of NTM up to its species level should gain importance in all TB reference Laboratories. [ABSTRACT FROM AUTHOR]

Nutrition support has become an important therapeutic intervention for improving outcomes in hospitalized patients. The present study was carried out with the objective of studying appropriate packaging and storage studies of enteral formulation from natural sources comprising finger millet (Eleusine coracana), soyprotein isolate, tomato powder, carrot powder, anne greens (Celosia argentea), groundnut oil and fish oil. Two type of packages--namely, polypropylene pouches and metallized polyester/polyethylene pouches--were used. The storage conditions were 27°C and 42°C at 65% relative humidity. The chemical, microbiological and sensory qualities of the formulation were assessed at regular intervals. The moisture sorption studies revealed that the formulation had an initial moisture content of 3.59%, which equilibrated to 13%. The product was acceptable at 64% relative humidity, after which it tend to become soggy. Analysis of peroxide value and free fatty acid content of the equilibrated samples revealed that moisture content of 4-5% was ideal for the storage of the formulation. An increase in the peroxide value and free fatty acid content was observed during 90-day storage period but the formulation was organoleptically acceptable. The microbial analysis of the formulation revealed an initial total bacterial count of 1.5×10² colony-forming units and increased to 7.9×10² colony-forming units (polypropylene) and 5.0×10² colony-forming units (metallized polyester/polyethylene). No fungal and coliform was detected at 90 days of storage. Thus it was concluded that the formulation can be stored for a period of 3 months at 27°C and 65% relative humidity. Such formulations can be primarily a supportive strategy to an active therapeutic intervention. [ABSTRACT FROM AUTHOR]

Nutrition support has become an important therapeutic intervention for improving outcomes in hospitalized patients. The present study was carried out with the objective of studying appropriate packaging and storage studies of enteral formulation from natural sources comprising finger millet (Eleusine coracana), soyprotein isolate, tomato powder, carrot powder, anne greens (Celosia argentea), groundnut oil and fish oil. Two type of packages--namely, polypropylene pouches and metallized polyester/polyethylene pouches--were used. The storage conditions were 27°C and 42°C at 65% relative humidity. The chemical, microbiological and sensory qualities of the formulation were assessed at regular intervals. The moisture sorption studies revealed that the formulation had an initial moisture content of 3.59%, which equilibrated to 13%. The product was acceptable at 64% relative humidity, after which it tend to become soggy. Analysis of peroxide value and free fatty acid content of the equilibrated samples revealed that moisture content of 4-5% was ideal for the storage of the formulation. An increase in the peroxide value and free fatty acid content was observed during 90-day storage period but the formulation was organoleptically acceptable. The microbial analysis of the formulation revealed an initial total bacterial count of 1.5 × 10² colony-forming units and increased to 7.9 × 10² colony-forming units (polypropylene) and 5.0 × 10² colony-forming units (metallized polyester/polyethylene). No fungal and coliform was detected at 90 days of storage. Thus it was concluded that the formulation can be stored for a period of 3 months at 27°C and 65% relative humidity. Such formulations can be primarily a supportive strategy to an active therapeutic intervention. [ABSTRACT FROM AUTHOR]

Abstract: The pancreatic stones (Pancreatic calculi) collected from patients suffering from chronic calcific pancreatitis were studied in a view to explore the constituents involved in the calcification. The calcified stones were characterized by XRD, EPR and IR spectroscopic techniques. The detailed studies indicate that these stones consist of calcium carbonate as a major component, besides phosphates and other protein content. The presence of aragonite phases in the biomineralized stones is also discussed. The EPR spectra gave an evidence of the presence of traces of manganese in different oxidation states, which is used as one of the EPR probes in the present work. The samples were sintered at different temperatures to remove all the organic matter, and their EPR spectra have been studied to obtain detailed information regarding the changes in the symmetry of these stone samples. The X-irradiated sample was also characterized by EPR and the resonance signals are attributed to freely rotating CO2 − radicals. The infrared spectrum reveals the presence of many organic bands corresponding to the protein amides. [Copyright &y& Elsevier]