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Background: Locomotor disorders and infections by Escherichia coli represent major concerns to the poultry industry worldwide. Avian pathogenic E. coli (APEC) is associated with extraintestinal infections leading to respiratory or systemic disease known as colibacillosis. The most common lesions seen in cases of colibacillosis are perihepatitis, airsacculitis, pericarditis, peritonitis/salpingitis and arthritis. These diseases are responsible for significant economic losses in the poultry industry worldwide. E. coli has been recently isolated from vertebral osteomyelitis cases in Brazil and there are no data on molecular and phenotypic characteristics of E. coli strains isolated from lesions in the locomotor system of broilers. This raised the question whether specific E. coli strains could be responsible for bone lesions in broilers. The aim of this study was to assess these characteristics of E. coli strains isolated from broilers presenting vertebral osteomyelitis and arthritis in Brazil. Results: Fifteen E. coli strains from bone lesions were submitted to APEC diagnosis and setting of ECOR phylogenic group, O serogroup, flagella type, virulence genes content, genetic patterns by Pulsed Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). In addition, bacterial isolates were further characterized through a lethality test, serum resistance test and antibiotic resistance profile. E. coli strains harbored different genetic pattern as assessed by PFGE, regardless of flock origin and lesion site. The strains belonged to seven sequence types (STs) previously described (ST117, ST101, ST131, ST 371 and ST3107) or newly described in this study (ST5766 and ST5856). ECOR group D (66.7 %) was the most frequently detected. The strains belonged to diverse serogroups (O88, O25, O12, and O45), some of worldwide importance. The antibiotic resistance profile confirmed strains' diversity and revealed a high proportion of multidrug-resistant strains (73 %), mainly to quinolones and beta-lactams, including third generation cephalosporin. The percentage of resistance to tetracycline was moderate (33 %) but always associated with multidrug resistance. Conclusions: Our results demonstrated that vertebral osteomyelitis and arthritis in broilers can be associated with highly diverse E. coli based on molecular and phenotypic characteristics. There was no specific virulence patterns of the E. coli strains associated with vertebral osteomyelitis or arthritis. Also, E. coli strains were frequently multidrug resistant and belonged to STs commonly shared by APEC and human ExPEC strains. [ABSTRACT FROM AUTHOR]

The atrophic rhinitis (AR) and pneumonia are among the most frequently diagnosed respiratory diseases in swine production systems and can lead to significant economic losses. The aim of this study was to determine the occurrence and degree of AR and pneumonia in hybrids and undefined breed swine herds submitted to intensive farming system and check if there is an association between the occurrence of these diseases in the herds. For this, we used 30 swines hybrids (Group I) and 30 undefined breed swines (Group II) from intensive farming system. The index of atrophic rhinitis (IAR) of herds for Groups I and II were, respectively, 1.00 and 0.57. In Group I, 20.8% (5/24) of the pigs presented pneumonia, while none of the animals in Group II exhibited this pathology. There was no correlation between the presence of atrophic rhinitis and pneumonia for the studied animals. These are the first data on the occurrence of these diseases in swines of Piauí State, Brazil. We demonstrated that these pathologies are present in animals from intensive farming systems and that occurrence varied according to racial type, being the hybrid swines the most affected animals by AR and pneumonia. [ABSTRACT FROM AUTHOR]

Amiloidosis is reported in a common shelduck (Tadorna tadorna) and in a white swan (Cygnus olor). Histologically, eosinophilic material suggestive of amyloid was predominantly observed in the basal membrane of the renal glomeruli, tubules and in the liver. The Congo red special stain with and without treatment by potassium permanganate confirmed the positivity for amyloid AA. [ABSTRACT FROM AUTHOR]

Endoparasitosis by spirurides in the digestive system of backyard chicken is reported. The proventricle wall was thickened with dark red circular areas in the submucosa glands. The gizzard showed firm and yellowish-white nodules under koilin membrane containing intralesional cylindrical nematodes. Histopathology of the proventriculus showed glandular dilatation and intraluminal female spirurides consistent with Tetrameres spp. beneath lymphocytes and eosinophils infiltrate. The gizzard presented areas of necrosis and inflammatory cells surrounding spirurides transversal sections, compatible with C. hamulosa. The proventriculitis and ventriculitis caused by spirurides can lead to significant pathological changes and gastrointestinal disorders in chickens. [ABSTRACT FROM AUTHOR]

Background: Histomoniasis is a disease caused by Histomonas meleagridis, a flagellated protozoan that can cause severe necrotizing hepatitis and typhlitis in several bird species. The disease has a cosmopolitan distribution. In experimental infection, peacocks (Pavo spp.) showed susceptibility to histomoniasis, however there are few reports on natural histomoniasis in this species. In northeastern Brazil, reports about its occurrence in avian species are scarce and nonexistent in peacocks. Therefore, this report aims to describe the epidemiological and clinicopathological aspects of a histomoniasis case in a peacock (Pavo cristatus) in the Brazilian semiarid region. Case: A 3-month-old male peacock with a history of apathy and anorexia was attended in the Veterinary Hospital of the Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil. The animal was raised extensively in a farm without basic sanitary measures, also with a history of living with animals of different species. After clinical examination, in which intense apathy and weight loss were confirmed, the bird was submitted to emergency therapeutic measures, however there was no clinical improvement and the bird died. At necropsy, intense diffuse bilateral necrotizing typhlitis and multifocal to coalescent necrotizing hepatitis were observed. Fragments of the organs were collected in 10% neutral buffered formalin for histopathological analysis and cecal content were collected for microbiological analysis. Histopathology of the cecum revealed transmural necrotizing typhlitis associated with myriads of trophozoites morphologically compatible with Histomonas meleagridis. The same microorganisms were observed in association with necrotizing hepatitis lesions, which allowed the diagnosis of histomoniasis. Also, the bacterium Pantoea agglomerans was isolated from the cecal content. Discussion: The macroscopic and microscopic findings allowed the diagnosis and the first recording of histomoniasis in peacock in the semi-arid region of Rio Grande do Norte. The bird was raised in an extensive breeding without sanitary management, such as the use of anthelmintics, which may favor infection by the nematode Heterakis gallinarum, which transmits the protozoan Histomonas meleagridis. The contamination, which begins by the orofecal route, happens through the ingestion of eggs of the nematode contaminated with H. meleagridis that pass through the gastrointestinal tract, reaching the ceca and causing intense lesions in the organ, such as the bilateral transmural typhlitis that we observed in this case. From ceca, the protozoan has access to the bloodstream and reaches the liver, where it causes necrotic hepatitis, also present in the peacock. Both cecal and hepatic lesions were associated with myriads of microorganisms morphologically compatible with H. meleagridis, which allowed the diagnosis of the disease. The challenge in diagnosing this disease occurs mainly due to nonspecific clinical signs, such as apathy and weight loss, the only signs reported by the breeder and observed in this peacock. Confirmation of the occurrence of histomoniasis in any region is important to establish the disease among the differential diagnoses for the species, as in this case. Since this is the first report of peacock histomoniasis in the semi-arid region of Rio Grande do Norte, it is evident the need to consider the disease among possible diagnoses in cases of nonspecific symptoms and it also demonstrates the need to implement control and prophylaxis measures in peacock breeding aiming to avoid losses of birds and economic losses to the breeders and to promote animal welfare. [ABSTRACT FROM AUTHOR]

The aim was to study the occurrence of canine hepatozoonose in rural area municipality of Teresina, Piauí. Were used 384 dogs without distinction of breed, sex and age, from the rural area of Teresina-PI, of which samples were collected for parasitological examination. Of blood smears examined Hepatozoon canis was detected in 0.26% (1/384) of animals. The present study reports the first occurrence of H. canis in dogs from rural areas of Teresina, Piauí. [ABSTRACT FROM AUTHOR]